CN105647882A - Landscaping garbage decomposing agent and preparation method thereof - Google Patents

Landscaping garbage decomposing agent and preparation method thereof Download PDF

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Publication number
CN105647882A
CN105647882A CN201610129575.2A CN201610129575A CN105647882A CN 105647882 A CN105647882 A CN 105647882A CN 201610129575 A CN201610129575 A CN 201610129575A CN 105647882 A CN105647882 A CN 105647882A
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decomposition agent
garbage decomposition
afforestation
lignin
afforestation garbage
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田辉
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01014—Lignin peroxidase (1.11.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01074—Glucan 1,4-beta-glucosidase (3.2.1.74)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115—Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Processing Of Solid Wastes (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a landscaping garbage decomposing agent and a preparation method thereof. The landscaping garbage decomposing agent comprises, by weight percentage, 10-30% of lignin degrading enzyme, 10-30% of cellulose degrading enzyme, 20-40% of slurry and the balance of water. The preparation method includes: using a culture medium to culture microorganism strains, placing various strains into a constant-temperature shaker for oscillation culture for 24-48 hours, and adding sterile water for diluting after the culture. The landscaping garbage decomposing agent is low in cost, fast in degrading and capable of efficiently degrading landscaping garbage to allow the same to be decomposed into organic fertilizer and has profound significance and actual value.

Description

A kind of afforestation garbage decomposition agent and preparation method thereof
Technical field
The present invention relates to a kind of garbage decomposition agent, be specially a kind of afforestation garbage decomposition agent and preparation method thereof.
Background technology
Afforestation kind grass seeds flower in city, certain covered ground or decorate, here it is urban afforestation, urban afforestation is that serike is to improve the activity of urban environment, urban afforestation is as the also basic stitch in urban ecological system, urban ecological system has and is subject to external interference and destruction and restorable ability, it is simply that the restoring function of usually said urban ecological system. Urban ecological system has the effect being primarily due in city to afforest ecological environment of restoring function, the research of urban afforestation ecological environment is sought to make full use of urban afforestation ecological environment makes urban ecological system have restoring function, this critical nature of life of urban resident environmental quality can be improved, also affect the fame in a city.
For making greenbelt more aesthetically pleasing, it is often necessary to forest flowers and plants are pruned, thus produce substantial amounts of afforestation rubbish. Afforestation rubbish mainly includes deadwood, falls leaves and cut the grass on a lawn, the garbage of shrub or branch, leaves etc. This rubbish is different from domestic waste, and it is made up of cellulose, hemicellulose and lignin, directly burns, not only pollutant atmosphere, and calorific value is low, is not easy to utilize; If carried out landfill disposal, although the ability of soil self can be relied on to be degraded, but degradation time is long, consuming the nutrient in soil, utilization rate is low; If it is banked up for a long time, not only land occupation, and also as time goes by, heap glove self fermentation, produce stench and percolate, heavy damage surrounding enviroment, people are produced extreme influence orthobiosis.
Summary of the invention
In order to overcome the problems referred to above, it is an object of the invention to provide a kind of afforestation garbage decomposition agent and preparation method thereof, the afforestation distintegrant microbial inoculum cost prepared by preparation method of the present invention is low, degradation speed is fast, can efficient degradation afforestation rubbish so that it is fast rotting is degraded into fertilizer.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of afforestation garbage decomposition agent, it includes following raw material composition by weight percentage:
Lignin-degrading enzymes 10-30%, cellulose degrading enzyme 10-30%, serosity 20-40%, surplus is water.
As the preferred forms of the present invention, it includes following raw material composition by weight percentage:
Lignin-degrading enzymes 20%, cellulose degrading enzyme 20%, serosity 20%, water 40%.
Further, described lignin-degrading enzymes is produce one or more any mixture in lignoenzyme, laccase, lignin peroxidase, cellulase.
Further, described cellulose degrading enzyme is the one in trichoderma (Trichoderma), aspergillus (Aspergillus), Penicillium (Penicillium).
Further, described serosity is the mixing of yeast and water.
Further, described saccharomycetic concentration is 40-60%.
As the most preferred embodiment of present embodiment, described saccharomycetic concentration is 50%.
A kind of preparation method of afforestation garbage decomposition agent, including:
Use culture medium to cultivate mentioned microorganism strain, various strains are put into vibration in constant-temperature table and cultivates 24-48 hour, add sterilized water after cultivation and be diluted to bacterium solution, bacterium solution is adsorbed onto on carrier, makes afforestation garbage decomposition agent.
The invention has the beneficial effects as follows: the afforestation garbage decomposition agent of the present invention is a kind of microbial inoculum being made up of multi-cultur es, utilize normal fermentation equipment, itself and afforestation rubbish is made to be sufficiently mixed, under aerobic condition, by different strain synergism, Direct Resolution constitutes the fallen leaves of afforestation rubbish, hay etc., and rot these afforestation rubbish to decompose after ripening, produce a large amount of humus, organic matter and trace element etc., constitute the fertilizer of useful plants growth, achieve the transformation of changing waste into resources, be truly realized no pollution, zero-emission; Afforestation garbage decomposition agent prepared by the present invention, microbial inoculum cost is low, and degradation speed is fast, it is possible to efficient degradation afforestation rubbish so that it is fast rotting is degraded into fertilizer, has profound significance and realistic price.
Detailed description of the invention
In order to make those skilled in the art be better understood from the technical scheme in the application; below in conjunction with embodiment, technical scheme is clearly and completely described; obviously; described embodiment is only a part of embodiment of the application; based on the embodiment in the application; the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, all should belong to the scope of the application protection.
Embodiment one
A kind of afforestation garbage decomposition agent, it includes following raw material composition by weight percentage: lignin-degrading enzymes 30%, cellulose degrading enzyme 30%, serosity 40%, lignin-degrading enzymes is lignin peroxidase, cellulose degrading enzyme is trichoderma, and serosity is the mixing of yeast and water, and saccharomycetic concentration is 60%.
The preparation method of afforestation garbage decomposition agent, including: use culture medium to cultivate mentioned microorganism strain, various strains are put into vibration in constant-temperature table cultivate 24 hours, add appropriate amounts of sterilized water after cultivation and be diluted to bacterium solution, bacterium solution is adsorbed onto on carrier, makes afforestation garbage decomposition agent.
Embodiment two
A kind of afforestation garbage decomposition agent, it includes following raw material composition by weight percentage: lignin-degrading enzymes 10%, cellulose degrading enzyme 10%, serosity 20%, water 60%, lignin-degrading enzymes is any mixing producing lignoenzyme and laccase, and cellulose degrading enzyme is aspergillus, serosity is the mixing of yeast and water, and described saccharomycetic concentration is 40%.
The preparation method of afforestation garbage decomposition agent, including: use culture medium to cultivate mentioned microorganism strain, various strains are put into vibration in constant-temperature table cultivate 48 hours, add appropriate amounts of sterilized water after cultivation and be diluted to bacterium solution, bacterium solution is adsorbed onto on carrier, makes afforestation garbage decomposition agent.
Embodiment three
A kind of afforestation garbage decomposition agent, it includes following raw material composition by weight percentage: lignin-degrading enzymes 20%, cellulose degrading enzyme 20%, serosity 20%, water 40%, lignin-degrading enzymes is any mixing producing lignoenzyme and laccase, and cellulose degrading enzyme is aspergillus, serosity is the mixing of yeast and water, and described saccharomycetic concentration is 40%.
The preparation method of afforestation garbage decomposition agent, including: use culture medium to cultivate mentioned microorganism strain, various strains are put into vibration in constant-temperature table cultivate 48 hours, add appropriate amounts of sterilized water after cultivation and be diluted to bacterium solution, bacterium solution is adsorbed onto on carrier, makes afforestation garbage decomposition agent.
Afforestation garbage decomposition agent provided by the invention and preparation method thereof, the afforestation distintegrant microbial inoculum cost prepared by preparation method of the present invention is low, and degradation speed is fast, it is possible to efficient degradation afforestation rubbish so that it is fast rotting is degraded into fertilizer.
The afforestation rubbish of 400kg is pulverized and separating twice for the first time, it is divided into five groups, often organize 100kg, first group of natural decomposition, decompose after second group of addition chicken manure, the 3rd group of distintegrant added in embodiment one, the 4th group of distintegrant added in embodiment two, the 5th group of distintegrant added in embodiment three, decomposes in fermentation vat, and contrast and experiment is: each group of 45 days small part is decomposed, second group of 45 this decomposition of space-based, 3rd group is all decomposed for 32 days, and the 4th group is all decomposed for 30 days, and the 5th group is all decomposed for 27 days.
The invention is not restricted to the details of above-mentioned one exemplary embodiment, and when without departing substantially from the spirit of the present invention or basic feature, it is possible to realize the present invention in other specific forms. Therefore, no matter from which point, embodiment all should be regarded as exemplary, and be nonrestrictive, description should be made as a whole by those skilled in the art, technical scheme in each embodiment can also through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that, the scope of the invention rather than described above limits, it is intended that all changes in the implication of the equivalency dropping on claim and scope included in the present invention.

Claims (8)

1. an afforestation garbage decomposition agent, it is characterised in that it includes following raw material composition by weight percentage: lignin-degrading enzymes 10-30%, cellulose degrading enzyme 10-30%, serosity 20-40%, surplus is water.
2. a kind of afforestation garbage decomposition agent according to claim 1, it is characterised in that it includes following raw material composition by weight percentage: lignin-degrading enzymes 20%, cellulose degrading enzyme 20%, serosity 20%, water 40%.
3. a kind of afforestation garbage decomposition agent according to claim 1 and 2, it is characterised in that described lignin-degrading enzymes is produce one or more mixture in lignoenzyme, laccase, lignin peroxidase, cellulase.
4. a kind of afforestation garbage decomposition agent according to claim 1 and 2, it is characterised in that described cellulose degrading enzyme is the one in trichoderma, aspergillus, Penicillium.
5. a kind of afforestation garbage decomposition agent according to claim 1 and 2, it is characterised in that described serosity is the mixture of yeast and water.
6. a kind of afforestation garbage decomposition agent according to claim 5, it is characterised in that described saccharomycetic concentration is 40-60%.
7. a kind of afforestation garbage decomposition agent according to claim 6, it is characterised in that described saccharomycetic concentration is 50%.
8. various strains are put into vibration in constant-temperature table and are cultivated 24-48 hour, add sterilized water dilution after cultivation by the preparation method of an afforestation garbage decomposition agent, it is characterised in that make through method below: use culture medium microorganisms strain.
CN201610129575.2A 2016-03-08 2016-03-08 Landscaping garbage decomposing agent and preparation method thereof Pending CN105647882A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109626555A (en) * 2019-01-21 2019-04-16 四川清和科技有限公司 A kind of method of fast degradation water body withered fallen leaf

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1509592A (en) * 2002-12-22 2004-07-07 浙江大学 Method for degrading lawn withered grass layer using microbe
CN1810735A (en) * 2006-02-23 2006-08-02 湖南大学 Decay promoting ferment and its application in composting agricultural waste
CN101987794A (en) * 2009-08-06 2011-03-23 南开大学 Method for degrading landscaping garbage to produce organic fertilizer by utilizing high-efficiency mixed bacteria
CN102517217A (en) * 2011-12-27 2012-06-27 何颖霞 Environment-friendly straw fermenting agent
CN102559508A (en) * 2011-08-17 2012-07-11 大连理工大学 Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1509592A (en) * 2002-12-22 2004-07-07 浙江大学 Method for degrading lawn withered grass layer using microbe
CN1810735A (en) * 2006-02-23 2006-08-02 湖南大学 Decay promoting ferment and its application in composting agricultural waste
CN101987794A (en) * 2009-08-06 2011-03-23 南开大学 Method for degrading landscaping garbage to produce organic fertilizer by utilizing high-efficiency mixed bacteria
CN102559508A (en) * 2011-08-17 2012-07-11 大连理工大学 Trichodermaviride used for producing cellulose degrading enzyme and its application in urban landscaping waste degradation
CN102517217A (en) * 2011-12-27 2012-06-27 何颖霞 Environment-friendly straw fermenting agent

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109626555A (en) * 2019-01-21 2019-04-16 四川清和科技有限公司 A kind of method of fast degradation water body withered fallen leaf

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