CN1509592A - Method for degrading lawn withered grass layer using microbe - Google Patents
Method for degrading lawn withered grass layer using microbe Download PDFInfo
- Publication number
- CN1509592A CN1509592A CNA021588465A CN02158846A CN1509592A CN 1509592 A CN1509592 A CN 1509592A CN A021588465 A CNA021588465 A CN A021588465A CN 02158846 A CN02158846 A CN 02158846A CN 1509592 A CN1509592 A CN 1509592A
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- China
- Prior art keywords
- litter layer
- spore
- lignin
- lawn
- rich
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 9
- 244000025254 Cannabis sativa Species 0.000 title claims abstract description 7
- 230000000593 degrading effect Effects 0.000 title claims 2
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 229940088598 enzyme Drugs 0.000 claims abstract description 11
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 8
- 241000233866 Fungi Species 0.000 claims abstract description 7
- 239000000725 suspension Substances 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- 229920002678 cellulose Polymers 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 238000000354 decomposition reaction Methods 0.000 claims description 5
- 229920005610 lignin Polymers 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 229920001503 Glucan Polymers 0.000 claims description 3
- 108010029541 Laccase Proteins 0.000 claims description 3
- 108010054320 Lignin peroxidase Proteins 0.000 claims description 3
- 108010059896 Manganese peroxidase Proteins 0.000 claims description 3
- 108010047754 beta-Glucosidase Proteins 0.000 claims description 3
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 3
- 230000001717 pathogenic effect Effects 0.000 claims description 3
- 231100000255 pathogenic effect Toxicity 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 230000001568 sexual effect Effects 0.000 claims description 3
- 230000004763 spore germination Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 230000006378 damage Effects 0.000 abstract description 5
- 238000005507 spraying Methods 0.000 abstract description 3
- 230000035558 fertility Effects 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 26
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 208000014674 injury Diseases 0.000 description 4
- 239000007921 spray Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
Abstract
A method for using microbes to degradate the withered grass layer on lawn includes such steps as choosing the fungus strain which has high activity of extracellular lignin-degradating enzyme and cellulase and high growth speed, solid or liquid fermenting to obtain a great deal of spores or mycelia, and spraying them on said withered grass layer directly or after they are used to prepare the suspension. Its advantages are high effect, no damage to lawn and improving fertility.
Description
Technical field the present invention relates to a kind of technical method that utilizes microbial degradation lawn litter layer, drafts a document level ground maintenance and management domain.
The background technology litter layer is withered old or fall down also root, stem, the leaf of undecomposed turfgrass and be accumulated in and form on the topsoil by some, it is one deck organic layer between turfgrass and soil, its Main Ingredients and Appearance is lignin and cellulose, and is difficult by natural decomposition.The too much accumulation of litter layer can hinder the infiltration and the exchange gas of moisture in the lawn, and isolated new root stretches into soil, causes root water uptake, inhales fertile and expiratory dyspnea, encourage the breeding of pathogenic bacteria, cause lawn be easy to get disease, insect pest, the lawn apparent mass is descended, finally cause the lawn to degenerate.Therefore must control the thickness of litter layer in the lawn maintenance management.The way of removing at present litter layer has ways such as apparatus punching, perpendicular cuts, top layer earthing (sand), winter baked wheaten cakes, but all take a lot of work effort, inefficiency, and to the injury in various degree of original lawn formation.
Summary of the invention is being removed take a lot of work on the litter layer effort, inefficiency in order to overcome the prior art means, and the deficiency that the lawn is had injury, the invention provides a kind of degrade method of litter layer of microorganism of utilizing, this method can not only degrade effectively litter layer, save labor, original lawn do not constituted injury, and litter layer can be converted into the fertilizer that can supply turfgrass to absorb, making it turns waste into wealth.
The principles of science of foundation of the present invention is: nature microorganism is of a great variety, the population decomposition of cellulose that has, the ability extra-heavy of lignin, and can both to be degraded be exactly an illustration to thick litter horizon in the remote, thickly forested mountains.Turfgrass belongs to herbaceous plant, and withered grass should be decomposed than woody plant is easier.In the zone that litter layer contacts with soil, humidity is higher, temperature changing amplitude is less relatively, be subjected to the intensity of UV sunlight width of cloth photograph faint, for conk provides comparatively adapt circumstance condition, litter layer and soil provide basic nutriment for fungi growth again simultaneously, the fungal spore of artificial infection, mycelia physical efficiency are sprouted on litter layer, are grown, utilize the outer lignin-degrading enzymes of born of the same parents of its mycelia secretion and lignin and the cellulose in the cellulase decomposition withered grass, thus degraded lawn litter layer.
The technical solution adopted for the present invention to solve the technical problems is: select the active high of the outer lignin-degrading enzymes (manganese-dependent peroxidase MnP, lignin peroxidase LiP and laccase Laccase) of born of the same parents, cellulase (endoglucanase EC3.2.1.4, glucan excision enzyme EC3.2.1.91 and beta-glucosidase EC3.2.1.21), fast growth, can produce a large amount of asexual spores or sexual spore, turfgrass not had the fungi strain of pathogenic effects; Obtain a large amount of spores or mycelium by solid fermentation or liquid fermentation mode; Conidial powder or the solid fermentation culture pulvis that is rich in spore directly are sprayed onto litter layer, or earlier spore or the pulvis that is rich in spore are configured to be sprayed onto litter layer behind the suspension, or directly will be rich in mycelial liquid fermentation liquid and be sprayed onto litter layer, can in suspension or liquid fermentation liquid, add the nutriment that can promote spore germination and mycelial growth; Fungi is grown on litter layer, utilize the outer lignin-degrading enzymes of born of the same parents of its mycelia secretion and lignin and the cellulose in the cellulase decomposition withered grass, reach the purpose of degraded lawn litter layer.
The invention has the beneficial effects as follows: but the microorganism formulation batch production production of degraded litter layer, use (spraying) convenient, do not need operations such as earthing (sand), baked wheaten cake, punching or perpendicular cuts, original lawn is not constituted injury, litter layer is converted into organic matter after decomposing on the spot, can increase soil fertility.
Embodiment
1. seed selection fungi strain.Adopt one of methods such as directed screening, physical mutagenesis, mutagenesis, genetic recombination, gene engineering or several method combination seed selection to meet the fungi strain of following condition simultaneously:
1) the outer lignin-degrading enzymes (manganese-dependent peroxidase MnP, lignin peroxidase LiP and laccase Laccase) of born of the same parents, cellulase (endoglucanase EC3.2.1.4, glucan excision enzyme EC3.2.1.91 and beta-glucosidase EC3.2.1.21) is active high;
2) fast growth can produce a large amount of asexual spores or sexual spore;
3) turfgrass there is not pathogenic effects.
2. fermentation.
1) obtains a large amount of spore or mycelium by solid fermentation; Or
2) obtain a large amount of mycelium by liquid fermentation.
3. preparation processing.
1) with the spore on the spore gatherer collection solid fermentation culture; Or
2), directly obtain to be rich in the pulvis of spore with solid fermentation culture low temperature drying, pulverizing; Or
3) directly obtain to be rich in mycelial liquid fermentation liquid.This zymotic fluid should use as early as possible, should not hide for a long time.
4. spray.
1) directly spore or the pulvis that is rich in spore are sprayed onto litter layer; Or
2) earlier spore or the pulvis that is rich in spore are configured to suspension, can add the nutriment that can promote spore germination and mycelial growth, be sprayed onto litter layer; Or
3) directly will be rich in mycelial liquid fermentation liquid and be sprayed onto litter layer, can add the nutriment that can promote mycelial growth.
5. technical essential and points for attention.
1) to spray season be the 4-10 month to the best of microorganism formulation, because of temperature is higher, helps conk therebetween;
2) time of spraying should not be chosen in sunlight strong noon, in order to avoid ultraviolet ray kills and wounds fungal spore or mycelium;
3) spray the relief litter layer and keep certain humidity, in order to conk.
Claims (3)
1. method of removing the lawn litter layer, utilize the microorganism litter layer of degrading, it is characterized in that: select outer lignin-degrading enzymes of born of the same parents and cellulase active height, fast growth, can produce a large amount of asexual spores or sexual spore, turfgrass do not had the fungi strain of pathogenic effects; Obtain a large amount of spores or mycelium by solid fermentation or liquid fermentation mode; Conidial powder or the solid fermentation culture pulvis that is rich in spore directly are sprayed onto litter layer, or earlier spore or the pulvis that is rich in spore are configured to be sprayed onto litter layer behind the suspension, or directly will be rich in mycelial liquid fermentation liquid and be sprayed onto litter layer, can in suspension or liquid fermentation liquid, add the nutriment that can promote spore germination and mycelial growth; Utilize the outer lignin-degrading enzymes of born of the same parents of hypha,hyphae secretion and lignin and the cellulose in the cellulase decomposition withered grass, degraded lawn litter layer.
2. the outer lignin-degrading enzymes of the born of the same parents described in claims 1 comprises manganese-dependent peroxidase MnP, lignin peroxidase LiP and laccase Laccase.
3. the cellulase described in claims 1 comprises endoglucanase EC3.2.1.4, glucan excision enzyme EC3.2.1.91 and beta-glucosidase EC3.2.1.21.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021588465A CN1509592A (en) | 2002-12-22 | 2002-12-22 | Method for degrading lawn withered grass layer using microbe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021588465A CN1509592A (en) | 2002-12-22 | 2002-12-22 | Method for degrading lawn withered grass layer using microbe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1509592A true CN1509592A (en) | 2004-07-07 |
Family
ID=34237193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021588465A Pending CN1509592A (en) | 2002-12-22 | 2002-12-22 | Method for degrading lawn withered grass layer using microbe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1509592A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329512A (en) * | 2010-06-09 | 2012-01-25 | 福特全球技术公司 | Cut sheet stock mycelium and method |
| CN102719483A (en) * | 2012-05-02 | 2012-10-10 | 中国热带农业科学院热带生物技术研究所 | Method for improving biogas yield through plant fermentation |
| CN105647882A (en) * | 2016-03-08 | 2016-06-08 | 田辉 | Landscaping garbage decomposing agent and preparation method thereof |
| CN113832040A (en) * | 2021-09-28 | 2021-12-24 | 北京林业大学 | Bacterium agent and application thereof in degrading lawn cumquat layer |
-
2002
- 2002-12-22 CN CNA021588465A patent/CN1509592A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329512A (en) * | 2010-06-09 | 2012-01-25 | 福特全球技术公司 | Cut sheet stock mycelium and method |
| CN102329512B (en) * | 2010-06-09 | 2016-06-01 | 福特全球技术公司 | The sheet stock mycelium of cutting and method |
| CN102719483A (en) * | 2012-05-02 | 2012-10-10 | 中国热带农业科学院热带生物技术研究所 | Method for improving biogas yield through plant fermentation |
| CN102719483B (en) * | 2012-05-02 | 2016-02-03 | 中国热带农业科学院热带生物技术研究所 | A kind of method improving plant fermentation biogas output |
| CN105647882A (en) * | 2016-03-08 | 2016-06-08 | 田辉 | Landscaping garbage decomposing agent and preparation method thereof |
| CN113832040A (en) * | 2021-09-28 | 2021-12-24 | 北京林业大学 | Bacterium agent and application thereof in degrading lawn cumquat layer |
| CN113832040B (en) * | 2021-09-28 | 2023-10-20 | 北京林业大学 | Microbial inoculum and application thereof in degradation of lawn hay layer |
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