CN105641054A - Propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, and preparation method of propolis honey paste - Google Patents
Propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, and preparation method of propolis honey paste Download PDFInfo
- Publication number
- CN105641054A CN105641054A CN201511035300.4A CN201511035300A CN105641054A CN 105641054 A CN105641054 A CN 105641054A CN 201511035300 A CN201511035300 A CN 201511035300A CN 105641054 A CN105641054 A CN 105641054A
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- CN
- China
- Prior art keywords
- subcritical
- folium mori
- extraction
- mori extract
- propolis
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention provides propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, belonging to the technical field of bee products. The propolis honey paste is prepared from the following components in parts by weight: 15-35 parts of mulberry leaf extract obtained by subcritical CO2 extraction, 50-80 parts of honey and 5-25 parts of propolis. The invention also provides a preparation method and an edible method of the propolis honey paste containing the mulberry leaf extract obtained by subcritical CO2 extraction. After the honey which has the functions of soothing the five internal organs, resolving the hundred toxins and complementing the hundred medicines is blended with the mulberry leaf extract obtained by subcritical CO2 extraction, the product edibility is improved, and the effective active ingredients in the honey, the propolis and mulberry leaves can be maintained; the propolis honey paste has the functions of dispelling wind and heat as well as improving the immunity of the organism, and also has a certain effect of relieving a sore throat, nasal obstruction and the like caused by a cold; the mulberry leaf extract is extracted by a subcritical CO2 extraction method, so that the effective active ingredients in fresh ginger can be maintained, the operation is simple, and the extraction rate is high.
Description
Technical field
The present invention relates to bee product technical field, specifically a kind of containing subcritical CO2Bee glue honeyed paste of Folium Mori extract extracted and preparation method thereof.
Background technology
Rich in carbohydrate, protein, aminoacid, vitamins and other nutritious components in Mel in existing technology, Mel is also rich in immunoglobulin, amylase, chromocor compound, the active polysaccharide isoreactivity factor. Containing several physiological active substances such as flavones in mulberry leaves, mulberry leaf polyphenol, polysaccharides of Folium Mori, DNJ, GABA in Folium Mori extract, it is used for preventing and treating cardiovascular and cerebrovascular disease, hyperlipemia, diabetes and defying age. China's traditional medicine thinks that Folium Mori extract is a kind of integration of edible and medicinal herbs beverage simultaneously, regulates blood glucose, dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, liver heat removing and eyesight improving.
Summary of the invention
In view of this, it is desirable to provide a kind of subcritical CO2The Folium Mori extract extracted.
For reaching above-mentioned purpose, the technical scheme is that and be achieved in that: a kind of subcritical CO2The Folium Mori extract extracted, subcritical CO2Extraction comprises the steps:
A Folium Mori are cleaned by () after, vacuum drying, adopt pulverizer that Folium Mori are carried out pulverization process and obtain Folium Mori powder;
B the screen cloth of the Folium Mori powder 30-50 order after pulverizing is sieved by ();
(c) subcritical CO2Extract: extracting pressure 5-15MPa, extraction temperature 15-30 DEG C, CO2Flow 160-190L/h, extraction time 1-4h, obtain Folium Mori extract primary product;
D () carries out secondary separation then through rectifying column, separating pressure is 10-30MPa, separation temperature 40-60 DEG C, obtains Folium Mori extract product through processed.
Further, described step (b) is sieved with the screen cloth of 40 orders.
Further, described step (c) extracting pressure 9.5MPa, extraction temperature 26 DEG C, CO2Flow 185L/h, extraction time 2h.
Further, described step (d) separating pressure is 19MPa, separation temperature 50.5 DEG C.
Another that the invention solves the problems that technical problem is that: provides above-mentioned containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted.
Above-mentioned containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, including each component of following parts by weight: subcritical CO2Folium Mori extract 15-35 part of being obtained by extraction, Mel 50-80 part, propolis 5-25 part.
Further, including each component of following parts by weight: subcritical CO2Folium Mori extract 20-30 part of being obtained by extraction, Mel 50-70 part, propolis 10-20 part.
Further, including each component of following parts by weight: subcritical CO2The Folium Mori extract being obtained by extraction 25 parts, Mel 60 parts, propolis 15 parts.
Another that the invention solves the problems that technical problem is that: provides above-mentioned containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted.
Above-mentioned containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted, comprises the steps:
(1) subcritical CO2Folium Mori extract is obtained by extraction;
(2) subcritical CO is weighed by above-mentioned parts by weight2Folium Mori extract, Mel and the propolis being obtained by extraction, carries out homogenizing after mixing;
(3) fill, pasteurize are carried out after homogenizing.
Further, the condition of pasteurize is 57 DEG C of sterilization 30min.
Another that the invention solves the problems that technical problem is that: provides above-mentioned containing subcritical CO2The eating method of the bee glue honeyed paste of the Folium Mori extract extracted.
Above-mentioned containing subcritical CO2The eating method of the bee glue honeyed paste of the Folium Mori extract extracted: once a day, each 10-20g, to take after dilute with water, the mass ratio of bee glue honeyed paste and water is 1:5-10, continuous 7 days.
Relative to prior art, present invention have the advantage that
(1) present invention has the advantage that and has the benefit effect that Mel settling five organs, solves hundred poison, and hundred medicines, and propolis and subcritical CO2The Folium Mori extract extracted is formulated together, improves product edibility, and the effective active composition in Mel, propolis, Folium Mori is retained simultaneously; Owing to adopting technique scheme, product of the present invention has dispelling wind and heat pathogens, improves the effect of immunity of organisms, swells and ache to alleviating the throat caused of catching a cold, and nasal obstruction etc. has certain effect.
(2) present invention adopts subcritical CO2The method extracted extracts Folium Mori extract, not only makes the effective active composition in Folium Mori be protected, and simple to operate, and extraction ratio is high.
Detailed description of the invention
It should be noted that when not conflicting, the embodiment in the present invention and the feature in embodiment can be mutually combined.
The present invention is described in detail below in conjunction with embodiment.
Embodiment one
A kind of containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, including each component of following weight: subcritical CO2The Folium Mori extract 25g that is obtained by extraction, Mel 60g, propolis 15g.
Above-mentioned containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted, comprises the steps:
(1) subcritical CO2Folium Mori extract, subcritical CO are obtained by extraction2Extraction step is as follows:
A 300g Folium Mori are cleaned by () after, vacuum drying, adopt pulverizer that Folium Mori are carried out pulverization process and obtain Folium Mori powder;
B Folium Mori powder after pulverizing is sieved by () with the screen cloth of 40 orders;
(c) subcritical CO2Extract: extracting pressure 9.5MPa, extraction temperature 26 DEG C, CO2Flow 185L/h, extraction time 2h, obtain Folium Mori extract primary product, and extraction ratio is 30.14%;
D () carries out secondary separation then through rectifying column, separating pressure is 19MPa, separation temperature 50.5 DEG C, obtains Folium Mori extract product through processed, and extraction ratio is 8.4%;
(2) subcritical CO is weighed by above-mentioned weight2Folium Mori extract, Mel and the propolis being obtained by extraction, carries out homogenizing after mixing;
(3) fill, pasteurize 57 DEG C sterilization 30min are carried out after homogenizing, after sterilization, warehouse-in of labelling.
Every part of product Folium Mori extract containing 25%, the propolis containing 15%.
Embodiment two
A kind of containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, including each component of following weight: subcritical CO2The Folium Mori extract 15g that is obtained by extraction, Mel 70g, propolis 15g.
Above-mentioned containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted, comprises the steps:
(1) subcritical CO2Folium Mori extract, subcritical CO are obtained by extraction2Extraction step is as follows:
A 300g Folium Mori are cleaned by () after, vacuum drying, adopt pulverizer that Folium Mori are carried out pulverization process and obtain Folium Mori powder;
B Folium Mori powder after pulverizing is sieved by () with the screen cloth of 30 orders;
(c) subcritical CO2Extract: extracting pressure 15MPa, extraction temperature 20 DEG C, CO2Flow 170L/h, extraction time 3h, obtain Folium Mori extract primary product, and extraction ratio is 28.8%;
D () carries out secondary separation then through rectifying column, separating pressure is 15MPa, separation temperature 45 DEG C, obtains Folium Mori extract product through processed, and extraction ratio is 7.9%;
(2) subcritical CO is weighed by above-mentioned weight2Folium Mori extract, Mel and the propolis being obtained by extraction, carries out homogenizing after mixing;
(3) fill, pasteurize 57 DEG C sterilization 30min are carried out after homogenizing, after sterilization, warehouse-in of labelling.
Every part of product Folium Mori extract containing 15%, the propolis containing 15%.
Embodiment three
A kind of containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, including each component of following weight: subcritical CO2The Folium Mori extract 30g that is obtained by extraction, Mel 50g, propolis 20g.
Above-mentioned containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted, comprises the steps:
(1) subcritical CO2Folium Mori extract, subcritical CO are obtained by extraction2Extraction step is as follows:
A 300g Folium Mori are cleaned by () after, vacuum drying, adopt pulverizer that Folium Mori are carried out pulverization process and obtain Folium Mori powder;
B Folium Mori powder after pulverizing is sieved by () with the screen cloth of 50 orders;
(c) subcritical CO2Extract: extracting pressure 8MPa, extraction temperature 30 DEG C, CO2Flow 190L/h, extraction time 1h, obtain Folium Mori extract primary product, and extraction ratio is 29.1%;
D () carries out secondary separation then through rectifying column, separating pressure is 18MPa, separation temperature 40 DEG C, obtains Folium Mori extract product through processed, and extraction ratio is 8.2%;
(2) subcritical CO is weighed by above-mentioned weight2Folium Mori extract, Mel and the propolis being obtained by extraction, carries out homogenizing after mixing;
(3) fill, pasteurize 57 DEG C sterilization 30min are carried out after homogenizing, after sterilization, warehouse-in of labelling.
Every part of product Folium Mori extract containing 30%, the propolis containing 20%.
Clinical trial
In order to confirm that this product swells and ache for the throat caused of catching a cold, nasal obstruction etc. has certain effect, has carried out clinical trial, and result of the test is in Table 1.
Now selecting the throat that 20 flu cause to swell and ache, have a stuffy nose patient, by embodiment 1-3 containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted gives each edible for patients respectively, and eating method is: once a day, and each 15g takes after dilute with water, and the mass ratio of bee glue honeyed paste and water is 1:6, continuous 7 days.Its therapeutic effect such as following table:
Table 1 clinical test results
| Contrast test | Rehabilitation | Alleviate | Invalid |
| Embodiment one | 19 | 1 | 0 |
| Embodiment two | 18 | 1 | 1 |
| Embodiment three | 18 | 2 | 0 |
| Bee glue honeyed paste without Folium Mori extract | 6 | 3 | 12 |
According to clinical trial prove the present invention with commercially available without compared with the bee glue honeyed paste of Folium Mori extract, there is dispelling wind and heat pathogens, improving the effect of immunity of organisms, swell and ache to alleviating the throat that flu causes, nasal obstruction etc. has certain effect.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (10)
1. a subcritical CO2The Folium Mori extract extracted, it is characterised in that: subcritical CO2Extraction comprises the steps:
A Folium Mori are cleaned by () after, vacuum drying, adopt pulverizer that Folium Mori are carried out pulverization process and obtain Folium Mori powder;
B the screen cloth of the Folium Mori powder 30-50 order after pulverizing is sieved by ();
(c) subcritical CO2Extract: extracting pressure 5-15MPa, extraction temperature 15-30 DEG C, CO2Flow 160-190L/h, extraction time 1-4h, obtain Folium Mori extract primary product;
D () carries out secondary separation then through rectifying column, separating pressure is 10-30MPa, separation temperature 40-60 DEG C, obtains Folium Mori extract product through processed.
2. subcritical CO according to claim 12The Folium Mori extract extracted, it is characterised in that: described step (b) is sieved with the screen cloth of 40 orders.
3. subcritical CO according to claim 12The Folium Mori extract extracted, it is characterised in that: described step (c) extracting pressure 9.5MPa, extraction temperature 26 DEG C, CO2Flow 185L/h, extraction time 2h.
4. subcritical CO according to claim 12The Folium Mori extract extracted, it is characterised in that: described step (d) separating pressure is 19MPa, separation temperature 50.5 DEG C.
5. one kind containing the subcritical CO described in any one of claim 1-42The bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: include each component of following parts by weight: subcritical CO2Folium Mori extract 15-35 part of being obtained by extraction, Mel 50-80 part, propolis 5-25 part.
6. one according to claim 5 is containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: include each component of following parts by weight: subcritical CO2Folium Mori extract 20-30 part of being obtained by extraction, Mel 50-70 part, propolis 10-20 part.
7. one according to claim 5 is containing subcritical CO2The bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: include each component of following parts by weight: subcritical CO2The Folium Mori extract being obtained by extraction 25 parts, Mel 60 parts, propolis 15 parts.
8. prepare as claimed in claim 5 containing subcritical CO for one kind2The method of the bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: comprise the steps:
(1) subcritical CO2Folium Mori extract is obtained by extraction;
(2) subcritical CO is weighed by above-mentioned parts by weight2Folium Mori extract, Mel and the propolis being obtained by extraction, carries out homogenizing after mixing;
(3) fill, pasteurize are carried out after homogenizing.
9. according to claim 8 containing subcritical CO2The preparation method of the bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: the condition of pasteurize is 57 DEG C of sterilization 30min.
10. one kind as claimed in claim 5 containing subcritical CO2The eating method of the bee glue honeyed paste of the Folium Mori extract extracted, it is characterised in that: once a day, each 10-20g, to take after dilute with water, the mass ratio of bee glue honeyed paste and water is 1:5-10, continuous 7 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511035300.4A CN105641054A (en) | 2015-12-31 | 2015-12-31 | Propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, and preparation method of propolis honey paste |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511035300.4A CN105641054A (en) | 2015-12-31 | 2015-12-31 | Propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, and preparation method of propolis honey paste |
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| Publication Number | Publication Date |
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| CN105641054A true CN105641054A (en) | 2016-06-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201511035300.4A Pending CN105641054A (en) | 2015-12-31 | 2015-12-31 | Propolis honey paste containing mulberry leaf extract obtained by subcritical CO2 extraction, and preparation method of propolis honey paste |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617049A (en) * | 2016-09-29 | 2017-05-10 | 广州市汇吉科技企业孵化器有限公司 | Propolis healthcare product with moringa oleifera leaf extract and method for preparing propolis healthcare product |
| CN107637817A (en) * | 2017-10-30 | 2018-01-30 | 雅安蒋氏蜜蜂园有限公司 | A kind of health-care honey powder and preparation method thereof |
| CN113115885A (en) * | 2021-04-27 | 2021-07-16 | 郑州市金色农业科技发展有限公司 | Mulberry leaf extract beverage |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1762428A (en) * | 2005-10-09 | 2006-04-26 | 上海现代药物制剂工程研究中心有限公司 | Method for extracting efficient component from mulberry leaf |
-
2015
- 2015-12-31 CN CN201511035300.4A patent/CN105641054A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762428A (en) * | 2005-10-09 | 2006-04-26 | 上海现代药物制剂工程研究中心有限公司 | Method for extracting efficient component from mulberry leaf |
Non-Patent Citations (2)
| Title |
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| 李雅晶等: "蜂胶桑叶复合片对2型糖尿病病人糖脂代谢的调节作用", 《安徽农业科学》 * |
| 陈意柯: "蜂蜜桑叶茶", 《蜜蜂杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617049A (en) * | 2016-09-29 | 2017-05-10 | 广州市汇吉科技企业孵化器有限公司 | Propolis healthcare product with moringa oleifera leaf extract and method for preparing propolis healthcare product |
| CN107637817A (en) * | 2017-10-30 | 2018-01-30 | 雅安蒋氏蜜蜂园有限公司 | A kind of health-care honey powder and preparation method thereof |
| CN113115885A (en) * | 2021-04-27 | 2021-07-16 | 郑州市金色农业科技发展有限公司 | Mulberry leaf extract beverage |
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