CN105638451A - Breeding method of low-temperature-resisting and weak-light-resisting female lines of Haiyang white cucumbers and application of breeding method - Google Patents
Breeding method of low-temperature-resisting and weak-light-resisting female lines of Haiyang white cucumbers and application of breeding method Download PDFInfo
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- CN105638451A CN105638451A CN201610015741.6A CN201610015741A CN105638451A CN 105638451 A CN105638451 A CN 105638451A CN 201610015741 A CN201610015741 A CN 201610015741A CN 105638451 A CN105638451 A CN 105638451A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention relates to a crop breeding technology and in particular relates to a breeding method of low-temperature-resisting and weak-light-resisting female lines of Haiyang white cucumbers, and belongs to the field of hybridization breeding. The breeding method comprises the following steps: firstly, selecting white cucumber varieties of Haiyang, and carrying out population improvement to separate selfing lines as female parents; then taking Northeast dry cucumber female line materials as male materials; after hybridization, carrying out a plurality of times of back-crossing; and selecting full-female germplasm of the low-temperature-resisting and weak-light-resisting Haiyang white cucumbers. Along the success of the breeding, the breeding method can be directly used for production, and a breeding progress of the Haiyang white cucumbers is accelerated, so that the breeding method has important practical significance on increasing additional values of the Haiyang white cucumbers and related products, accelerating industrialized development paces of the Haiyang white cucumbers, and further improving the brand awareness of the Haiyang white cucumbers.
Description
Technical field
The present invention relates to a kind of crop breeding technology, particularly relate to the selection of a kind of greenhouse tailored version " Haiyang White cucumber " female series, belong to cross-breeding field.
Background technology
" Haiyang White cucumber " is the fruit deeply liked by people, because the restriction of the factor such as its plant setting percentage is low, not low temperature resistant, hinders implant mass and the periodically supply of White cucumber. Cross-breeding was also once attempted in locality, Haiyang, but by the restriction of breeding material and breeding method, all unsuccessful.
Increase along with White cucumber demand, the research of Haiyang White cucumber cenospecies is in the urgent need to address, especially the new varieties of tolerance to low temperature and weak light are selected, it is suitable for chamber planting of surviving the winter, thus meeting the cycle supply of Haiyang White cucumber, this just requires to be innovated in original germ plasm resource, select the female series of a kind of tolerance to low temperature and weak light, the breeding process of Haiyang White cucumber cenospecies of chamber planting thus accelerating to be suitable for surviving the winter, increase the added value of Haiyang White cucumber and correlated product, promote the industrialized development paces of Haiyang White cucumber, and then improve Haiyang White cucumber brand recognition, there is higher realistic meaning.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is provided that the selection of tolerance to low temperature and weak light " Haiyang White cucumber " female series of a kind of suitable chamber planting. Locality, Haiyang White cucumber kind is isolated selfing line through population improvement and is made female parent, and male parent made by northeast dry land cucumber female series material, through repeatedly backcrossing after hybridizing, selects " Haiyang White cucumber " that meet target call and plants matter.
The technical scheme is that the selection of a kind of low temperature resistant low light tolerance " Haiyang White cucumber " female series, comprise the following steps:
1) Haiyang White cucumber population improvement:
1. basic population is collected: selects very heavy Huang, Sun Ge village White cucumber (White cucumber eight), stay lattice Shao Jia great Ye White cucumber (White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (White cucumber six), and send out city east Fang Wu White cucumber (White cucumber two), after selfing purifies respectively, colony based on reserving seed for planting;
2. miscegenation: by basic population miscegenation, select the fine individual plant possessing Haiyang White cucumber commodity from the next generation;
3. individual plant systematic breeding: the fine individual plant that previous step is selected adopts individual plant systematic breeding method, and each individual plant is each sowed 1 furrow becomes a family, the internal selfing of family is reserved seed for planting, and chooses defect individual;
4. choose described defect individual repeat step 2.-3. four time after, the final defect individual obtained obtains Haiyang White cucumber selfing line through selfing purification;
2) cross-breeding:
A. using aforementioned Haiyang White cucumber selfing line as female parent, hybridize using northeast dry land cucumber as male parent, it is thus achieved that F1 generation seed;
B. selecting both to possess Haiyang White cucumber biological character from F1 generation plant, setting percentage is high again simultaneously, cold-resistant individual plant 1-2;
C. using aforementioned White cucumber selfing line as recurrent parent, F1 generation individual plant selected in step B and aforementioned White cucumber selfing line, as nonrecurrent parent, are backcrossed by northeast dry land cucumber, select the low temperature resistant and low light level, individual plant 2-4 that setting percentage is high in backcross progeny;
D. repeat the accumulative 4-5 generation that backcrosses of step C, select the low temperature resistant and low light level, fine individual plant that setting percentage is high some, selfing purification can obtain Haiyang White cucumber female series of low temperature resistant low light tolerance.
On the basis of technique scheme, the present invention can also do following improvement.
Further, step 1) in Haiyang White cucumber population improvement 2. miscegenation, described in possess the fine individual plant of Haiyang White cucumber commodity, refer to sugar-preserved gourd length 16��21cm, reveal the green during melon color is white, melon is short, and melon skin is thin, the sweet crisp White cucumber of mouthfeel.
Further, step 2) also include step 3 after cross-breeding) contrast test, to step 2) selected kind matter plants experimentally, and to the yield of fine individual plant, adaptability, disease resistance, and/or quality and flavor is tested.
Described yield trials is the new low temperature resistant low light tolerance Haiyang White cucumber female series being bred as is carried out community under suitable cryogenic conditions repeat, and early yield is measured and adds up total output; The condition of described suitable low temperature is: Seedling Stage night 12-17 DEG C, daytime 17-22 DEG C; Yield positive results night 15-20 DEG C phase, daytime 20-25 DEG C.
Described quality and flavor is tested, for the quality of White cucumber is described analysis; Described disease resistance is tested, for judging its resistance to downy mildew, powdery mildew by the disease index of blade.
Described early yield, refers within a cucumber growth cycle, surveys the summation producing yield and the yield of the gathered in the crops Fructus Cucumidis sativi of bulk registration before gathering in the crops Fructus Cucumidis sativi the same day.
Further, described contrast test needs to set matched group, described matched group is selected from very heavy Huang, Sun Ge village White cucumber (White cucumber eight), stays lattice Shao Jia great Ye White cucumber (White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (White cucumber six), and/or sends out city east Fang Wu White cucumber (White cucumber two).
Carry out multispots trial demonstration, thus selecting to meet the kind matter of breeding objective requirement.
Further, step 2) the northeast dry land cucumber that adopts is the female series that lower temperature resistance is strong, growing way is strong; Step 2) hybridization before, it is necessary to described northeast dry land cucumber is purificated and rejuvenated.
Described purification and rejuvenation, refer to and select typical individual plant or single fringe from the breeding of Hybridization and degeneration, recover to improve its purity and kind property, make up to the measure of original seed standard.
In the breeding method of the present invention, northeast dry land cucumber is first as improveing object (donor) to Haiyang White cucumber (modified object, receptor) improve, the improvement for White cucumber provides new thinking, the great practical significance of the method and market prospect.
Second aspect present invention discloses the application in the White cucumber breeding of Haiyang of the aforementioned selection.
Further, described application is the method that the proceeds to application at Haiyang White cucumber cross-breeding that backcrosses.
The present invention quotes the method for backcross breeding first in the White cucumber germplasm innovation of Haiyang, is improve rapid and effective method not low temperature resistant, that setting percentage is low. Backcross after Haiyang White cucumber and the hybridization of northeast dry land cucumber 4-5 generation repeatedly, and wherein Haiyang White cucumber is as recurrent parent (receptor parent), and northeast dry land cucumber is nonrecurrent parent, the supplier of objective trait (low temperature resistant, gynoecy). Haiyang White cucumber is the object accepting improvement, its Comprehensive Traits is good, best in quality, long at local planting time, there are the potentiality of high yield, but deposit critical defect both ways, namely lower temperature resistance difference and setting percentage are low, but low temperature resistant and deep color of the leather also exists gene linkage, and backcross and there is the probability of gene recombinaton, therefore, when selecting, backcross and can reach to break the chain of objective trait gene and bad character gene. The end product backcrossed is the offspring of the Comprehensive Traits of the objective trait+recurrent parent of nonrecurrent parent.
The invention has the beneficial effects as follows: the selection-breeding success of tolerance to low temperature and weak light " Haiyang White cucumber " female series, not only it is used directly for producing, and accelerate the breeding process of " Haiyang White cucumber " cenospecies, this added value to adding Haiyang White cucumber and correlated product, promote the industrialized development paces of Haiyang White cucumber, and then improve Haiyang White cucumber brand recognition, there is higher realistic meaning.
Detailed description of the invention
Hereinafter principles of the invention and feature being described, example is served only for explaining the present invention, is not intended to limit the scope of the present invention.
The selection-breeding of example tolerance to low temperature and weak light " Haiyang White cucumber " female series
1, germ plasm resource collection, arrange and carry out the genetic improvement of colony
The White cucumber in 1.1 locality, Haiyang is collected, arranges, is classified
According to zones of different, peasant's planting habit for many years, conventional seed selection, has gradually formed respective different characteristic characteristic in certain circumstances. Collected Haiyang White cucumber local varieties of various trait: very heavy Huang, Sun Ge village White cucumber (White cucumber eight), stay lattice Shao Jia great Ye White cucumber (White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (White cucumber six), send out city east Fang Wu White cucumber (White cucumber two), carry out selfing purification.
The population improvement of 1.2 Haiyang White cucumber systematic genetic
By the native breed after purification, miscegenation, select the low temperature resistant and low light level from the next generation, fine individual plant that setting percentage is high, adopt individual plant systematic breeding method, each individual plant oneself sows 1 furrow becomes a family, and the internal selfing of family is reserved seed for planting menu strain, selected outstanding individual plant miscegenation again, 4 times successively, have chosen excellent, energetic Haiyang White cucumber selfing line.
2, backcross and proceed to
2.1 introduce northeast dry land cucumber female series (northeast dry land cucumber 8) low temperature resistant, allfemale type
From the dry land cucumber strain of northeast, screen lower temperature resistance is strong, growing way is strong female series (northeast dry land cucumber 8) and plant its feature of observation, purification and rejuvenation.
2.2 heterosis, hybrid vigors utilize
Female parent is done with selected Haiyang White cucumber selfing line, selected northeast dry land cucumber 8 is male parent, hybridization, selects the individual plant possessing target call (both to possess Haiyang White cucumber biological character from F1, setting percentage is high again simultaneously, cultivates cold-resistant individual plant under specified conditions) 1-2.
2.3 backcross breedings
Using selected White cucumber selfing line as recurrent parent, northeast dry land cucumber 8 as nonrecurrent parent, the selected individual plant of F1 respectively with Haiyang White cucumber hybridization between selfed lines, of future generation more respectively choosing meet individual plant 2-4 of target call, backcross 4-5 generation successively, and the fine individual plant that choosing meets target call is some; Haiyang White cucumber female series of low temperature resistant low light tolerance is obtained, emerald green white No. two of called after distinctness after selfing purification.
3, carry out contrast test, further determine that into seed selection matter
Plant experimentally observation distinct emerald green white No. two yield, adaptability, disease resistance and quality and flavor are judged. The selfing line being bred as being carried out community, suitable low temperature environment greenhouse repeat, early yield is measured and adds up total output; Adopt hedonic scoring system, its quality is done preliminary check analysis; Its resistance to downy mildew, powdery mildew is judged by the disease index of blade.
Carry out multispots trial demonstration, select to meet the kind matter of breeding objective requirement.
4, result of the test
4.1 yielding abilities
Measure the strain of cultivation, line-spacing in comparative test booth, obtain every mu of strain number; Diagonally take 3 points, every some successive survey 10 strain, statistics knot melon number, obtain average every strain knot melon number; Every kind is won 30 commodity fruits and is weighed, and calculates average single melon weight, and Production rate is calculated as follows: yield (kilogram/667m2)=mono-melon weight �� individual plant knot melon �� mu strain number. Compare with Haiyang White cucumber two of purified rejuvenation. Relatively comparison volume increase 33.6%, difference all reaches pole significant level.
Regional testing plot area 15-30m2, 667m2Yield=(cell production/plot area) �� 667. Relatively comparison volume increase more than 32.7%, total producing relatively compares volume increase more than 43.5%, and difference all reaches pole significant level.
Pilot production adopts multiple spot pilot production. Early yield is relatively comparison volume increase more than 29.7% respectively; Total output relatively compares volume increase more than 34.2%.
4.2 ratio of female
While carrying out variety test, regional testing and pilot production, full female plant and strong female plant are investigated. Distinct emerald green white No. two full female plant rates 95.2%, strong female plant rate 3.6%, hence it is evident that be better than comparison Haiyang White cucumber three (0 and 0.3%).
4.3 qualities
Adopting hedonic scoring system, 5 people participate in. Flavor quality by peel thickness, quality degree tender and crisp, sour-sweet, have the four indices such as free from extraneous odour, each score 10 points, full marks 40 points; Exterior quality investigation 5 melons, by color of the leather, melon length, 4 marking such as long, seeds chamber footpath melon transverse diameters, each count 10 points, full marks 40 points. Distinct emerald green white No. two respectively 36.8 and 39.3, hence it is evident that be better than comparison (Haiyang White cucumber No. two 29.5 and 30.3).
4.4 low temperature resistant and plant growing ways
It is better than comparison, suitable Protectorate cultivation. Its cold-resistant low light tolerance index is judged by aggregative indicatores such as 12 degrees Celsius of germination percentages, plant height, total blade area, stem is thick and dry matter weights. Observe to measure and find that distinct emerald green white No. two tolerance to low temperature and weak light relatively compare (Haiyang White cucumber two) and are obviously enhanced.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (4)
1. a selection for tolerance to low temperature and weak light Haiyang White cucumber female series, comprises the following steps:
1) Haiyang White cucumber population improvement:
1. basic population is collected: selects very heavy Huang, Sun Ge village White cucumber, stay lattice Shao Jia great Ye White cucumber, mountain strip White cucumber, Chu Ge village White cucumber, and sends out city east Fang Wu White cucumber, after selfing purifies respectively, and colony based on reserving seed for planting;
2. miscegenation: by basic population miscegenation, select the fine individual plant possessing Haiyang White cucumber commodity from the next generation;
3. individual plant systematic breeding: the fine individual plant that previous step is selected adopts individual plant systematic breeding method, and each individual plant is each sowed 1 furrow becomes a family, the internal selfing of family is reserved seed for planting, and chooses defect individual;
4. choose described defect individual repeat step 2.-3. four time after, the final defect individual obtained obtains Haiyang White cucumber selfing line through selfing purification;
2) cross-breeding:
A. using aforementioned Haiyang White cucumber selfing line as female parent, hybridize using northeast dry land cucumber as male parent, it is thus achieved that F1 generation seed;
B. selecting both to possess Haiyang White cucumber biological character from F1 generation plant, setting percentage is high again simultaneously, cold-resistant individual plant 1-2;
C. using aforementioned White cucumber selfing line as recurrent parent, F1 generation individual plant selected in step B and aforementioned White cucumber selfing line, as nonrecurrent parent, are backcrossed by northeast dry land cucumber, select the low temperature resistant and low light level, individual plant 2-4 that setting percentage is high in backcross progeny;
D. repeat the accumulative 4-5 generation that backcrosses of step C, select the low temperature resistant and low light level, fine individual plant that setting percentage is high some, selfing purification can obtain Haiyang White cucumber female series of tolerance to low temperature and weak light.
2. selection as claimed in claim 1, it is characterised in that step 1) in Haiyang White cucumber population improvement 2. miscegenation, the described fine individual plant possessing Haiyang White cucumber commodity, refers to sugar-preserved gourd length 16��21cm, reveals the green during melon color is white, melon is short, and melon skin is thin, the sweet crisp White cucumber of mouthfeel.
3. the application in the White cucumber cross-breeding of Haiyang of the selection described in claim 1-2 any claim.
4. apply as claimed in claim 3, it is characterised in that described application is the method that the proceeds to application at Haiyang White cucumber cross-breeding that backcrosses.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050693A (en) * | 2019-06-12 | 2019-07-26 | 武汉庆发禾盛农业发展有限公司 | A kind of selection of high-yield disease resisting watermelon seed |
| CN111149691A (en) * | 2020-03-20 | 2020-05-15 | 山东永盛农业发展有限公司 | Method for breeding weak light-resistant inbred line by utilizing tomato hybrid |
-
2016
- 2016-01-11 CN CN201610015741.6A patent/CN105638451B/en active Active
Non-Patent Citations (3)
| Title |
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| 周俊国等: "《园艺植物育种技术》", 31 August 2006 * |
| 山东海阳鲜明蔬菜研究所: ""山东海阳白黄瓜"", 《山东蔬菜》 * |
| 张天真: "《作物育种学总论 第3版》", 31 March 2014 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050693A (en) * | 2019-06-12 | 2019-07-26 | 武汉庆发禾盛农业发展有限公司 | A kind of selection of high-yield disease resisting watermelon seed |
| CN111149691A (en) * | 2020-03-20 | 2020-05-15 | 山东永盛农业发展有限公司 | Method for breeding weak light-resistant inbred line by utilizing tomato hybrid |
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