CN105638451B - A kind of selection of tolerance to low temperature and weak light Haiyang White cucumber female series and its application - Google Patents
A kind of selection of tolerance to low temperature and weak light Haiyang White cucumber female series and its application Download PDFInfo
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- CN105638451B CN105638451B CN201610015741.6A CN201610015741A CN105638451B CN 105638451 B CN105638451 B CN 105638451B CN 201610015741 A CN201610015741 A CN 201610015741A CN 105638451 B CN105638451 B CN 105638451B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The present invention relates to a kind of crop breeding technology, more particularly to a kind of selection of tolerance to low temperature and weak light type " Haiyang White cucumber " female series, belong to crossbreeding field.The present invention selects Haiyang locality White cucumber kind to isolate self-mating system work female parent through population improvement first, then makees male parent with northeast dry land cucumber female based material, and repeatedly backcrossing is passed through after being hybridized, and selects " Haiyang White cucumber " gynoecy germplasm of tolerance to low temperature and weak light.The success of seed selection of the present invention, not only it is used directly for producing, and accelerate " Haiyang White cucumber " breeding process, this to increase Haiyang White cucumber and correlated product added value, promote the industrialized development paces of Haiyang White cucumber, and then Haiyang White cucumber brand recognition is improved, have important practical significance.
Description
Technical field
The present invention relates to a kind of crop breeding technology, more particularly to a kind of greenhouse tailored version " Haiyang White cucumber " female series
Selection, belong to crossbreeding field.
Background technology
" Haiyang White cucumber " is the deep fruit liked by people, because low, not low temperature resistant etc. the factor of its plant setting percentage
Limitation, hinder the large-scale plantation of White cucumber and periodically supply.Crossbreeding is also once attempted in Haiyang locality, but by breeding material
The limitation of material and breeding method, not successfully.
With the increase of White cucumber demand, the research of Haiyang White cucumber cenospecies is in the urgent need to address, especially to select
The new varieties of tolerance to low temperature and weak light are brought out, chamber planting of suitably surviving the winter, so as to meet that the cycle of Haiyang White cucumber supplies, this is required
Innovated in original germ plasm resource, select a kind of female series of tolerance to low temperature and weak light, so as to accelerate greenhouse kind of suitably surviving the winter
The breeding process of Haiyang White cucumber cenospecies of plant, increase the added value of Haiyang White cucumber and correlated product, promote Haiyang yellow in vain
The industrialized development paces of melon, and then Haiyang White cucumber brand recognition is improved, there is higher realistic meaning.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of tolerance to low temperature and weak light " Haiyang White cucumber " of suitable chamber planting
The selection of female series.Haiyang locality White cucumber kind isolates self-mating system through population improvement and makees female parent, and northeast dry land cucumber is female
Property based material make male parent, after being hybridized through repeatedly backcrossing, select " Haiyang White cucumber " germplasm for meeting target call.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：A kind of low temperature resistant low light tolerance " Haiyang White cucumber " female
The selection of system, comprises the following steps：
1) Haiyang White cucumber population improvement：
1. basic population is collected：Select very heavy Huang, Sun Ge village White cucumber (White cucumber eight), stay lattice Shao Jia great Ye White cucumbers
(White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (White cucumber six), and hair city east Fang Wu White cucumbers are (white
Cucumber No. two), respectively after selfing purification, colony based on reserving seed for planting；
2. miscegenation：By basic population miscegenation, the fine individual plant for possessing Haiyang White cucumber commodity is selected from the next generation；
3. individual plant systematic breeding：The fine individual plant selected to previous step uses individual plant systematic breeding method, by each individual plant
Each 1 furrow of sowing turn into a family, are selfed and reserve seed for planting inside family, choose defect individual；
2. -3. four time 4. after choosing the defect individual repeat step, the defect individual finally obtained obtains through being selfed purifying
Obtain Haiyang White cucumber self-mating system；
2) crossbreeding：
A. using foregoing Haiyang White cucumber self-mating system as female parent, hybridized using northeast dry land cucumber as male parent, obtain F1
For seed；
B. select both possessed Haiyang White cucumber biological character from F1 generation plant, while setting percentage is high again, cold-resistant list
Strain 1-2；
C. using foregoing White cucumber self-mating system as recurrent parent, northeast dry land cucumber will enter as nonrecurrent parent in step B
The F1 generation individual plant of choosing is returned with foregoing White cucumber self-mating system, and the high list of low temperature resistant and dim light, setting percentage is selected in backcross progeny
Strain 2-4；
D. repeat step C backcrossing adds up 4-5 generations, selects the high fine individual plant of low temperature resistant and dim light, setting percentage some, from
Purifying is handed over to obtain Haiyang White cucumber female series of low temperature resistant low light tolerance.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, White cucumber population improvement in step 1) Haiyang is described to possess the excellent of Haiyang White cucumber commodity 2. in miscegenation
Good individual plant, refer to that sugar-preserved gourd grows 16~21cm, revealed the green during melon color is white, melon is short, and melon skin is thin, the crisp White cucumber of mouthfeel sweet tea.
Further, step 3) contrast test is also included after step 2) crossbreeding, the germplasm being selected in step 2) tries
Kind, the yield, adaptability, disease resistance of fine individual plant, and/or quality and flavor are tested.
The yield trials is to carry out appropriate cryogenic conditions to the low temperature resistant low light tolerance Haiyang White cucumber female series being newly bred as
Lower cell repeats, and early yield measures and added up total output；The condition of the appropriate low temperature is：12-17 DEG C of Seedling Stage night,
17-22 DEG C of daytime；Yield positive results 15-20 DEG C of night phase, 20-25 DEG C of daytime.
The quality and flavor experiment, for analysis is described to the quality of White cucumber；The disease resistance experiment, to pass through leaf
The disease index of piece judges it to downy mildew, the resistance of powdery mildew.
The early yield, refer within a cucumber growth cycle, survey the yield and before of production same day harvest cucumber
Bulk registration harvests the summation of the yield of cucumber.
Further, the contrast test needs to set control group, and the control group is selected from very heavy yellow, Sun Ge village White cucumber
(White cucumber eight), stay lattice Shao Jia great Ye White cucumbers (White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (white yellow
Melon six), and/or hair city east Fang Wu White cucumbers (White cucumber two).
Multispots trial demonstration is carried out, so as to select to meet the germplasm of breeding objective requirement.
Further, northeast dry land cucumber is the female series that lower temperature resistance is strong, growing way is strong used by step 2)；Step 2) hybridizes
Before, it is necessary to be purificated and rejuvenated to the northeast dry land cucumber.
The purification and rejuvenation, refer to selecting typical individual plant or single fringe from the breeding of Hybridization and degeneration, recover to improve it
Purity and kind property, make up to the measure of original seed standard.
In the breeding method of the present invention, northeast dry land cucumber is first as improvement object (donor) to Haiyang White cucumber (quilt
Improve object, acceptor) improved, provide new thinking, the great practical significance of this method and market for the improvement of White cucumber
Prospect.
Second aspect of the present invention discloses application of the foregoing selection in the White cucumber breeding of Haiyang.
Further, the application is transferred to application of the method in Haiyang White cucumber crossbreeding for backcrossing.
The present invention quote backcross breeding first in terms of the White cucumber germplasm innovation of Haiyang method, be improve it is not low temperature resistant,
The low rapid and effective method of setting percentage.For Haiyang White cucumber with being returned 4-5 generations repeatedly after the hybridization of northeast dry land cucumber, wherein Haiyang is yellow in vain
Melon is as recurrent parent (receptor parent), and northeast dry land cucumber is nonrecurrent parent, the offer of objective trait (low temperature resistant, gynoecy)
Person.Haiyang White cucumber is the object for receiving improvement, and its Comprehensive Traits is good, best in quality, is grown in local planting time, there is high yield
Potentiality, but deposit critical defect both ways, i.e. lower temperature resistance difference and setting percentage are low, but low temperature resistant there is gene company with deep color of the leather
Lock, and being returned there is the probability of genetic recombination, therefore in the case of selection, backcrossing can reach break objective trait gene with
Bad character gene it is chain.The end product of backcrossing is the Comprehensive Traits of objective trait+recurrent parent of nonrecurrent parent
Offspring.
The beneficial effects of the invention are as follows：The seed selection success of tolerance to low temperature and weak light " Haiyang White cucumber " female series, not only can be straight
Connect for producing, and accelerate the breeding process of " Haiyang White cucumber " cenospecies, this is to adding Haiyang White cucumber and related system
The added value of product, promote Haiyang White cucumber industrialized development paces, and then improve Haiyang White cucumber brand recognition, have compared with
High realistic meaning.
Embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
The seed selection of example tolerance to low temperature and weak light " Haiyang White cucumber " female series
1st, collection, arrangement and the genetic improvement for carrying out colony of germ plasm resource
The local White cucumber in 1.1 Haiyang is collected, arranged, classification
According to different zones, the planting habit of peasant for many years, conventional seed selection, has been gradually formed each not in certain circumstances
Same feature.Collected Haiyang White cucumber local varieties of various trait：Very heavy yellow, Sun Ge village White cucumber (White cucumber eight
Number), stay lattice Shao Jia great Ye White cucumbers (White cucumber three), mountain strip White cucumber, Chu Ge village White cucumber (White cucumber six), hair
City east Fang Wu White cucumbers (White cucumber two), carry out selfing purification.
The population improvement of 1.2 Haiyang White cucumber systematic genetics
By the native breed after purification, miscegenation, the high fine individual plant of low temperature resistant and dim light, setting percentage is selected from the next generation,
Using individual plant systematic breeding method, each individual plant oneself, which sows 1 furrow, turns into a family, is selfed menu strain of reserving seed for planting inside family, enters
Outstanding individual plant miscegenation again is selected, 4 times successively, have chosen excellent, energetic Haiyang White cucumber self-mating system.
2nd, backcrossing is transferred to
2.1 introduce low temperature resistant, allfemale type northeast dry land cucumber female series (northeast dry land cucumber 8)
The female series (northeast dry land cucumber 8) that lower temperature resistance is strong, growing way is strong are screened from the dry land cucumber strain of northeast and are planted
Its feature is observed, is purificated and rejuvenated.
2.2 heterosis, hybrid vigors utilize
Female parent is done with selected Haiyang White cucumber self-mating system, it is male parent to be selected in northeast dry land cucumber 8, hybridization, is selected from F1
The individual plant for possessing target call (had both possessed Haiyang White cucumber biological character, while setting percentage is high again, is cultivated under specified conditions resistance to
Cold individual plant) 1-2.
2.3 backcross breeding
Nonrecurrent parent is used as recurrent parent, northeast dry land cucumber 8 using selected White cucumber self-mating system, F1 is selected in individual plant
Respectively with Haiyang White cucumber hybridization between selfed lines, of future generation choosing meets individual plant 2-4 of target call respectively again, is returned 4-5 successively
Generation, the fine individual plant that choosing meets target call are some；Selfing obtains Haiyang White cucumber female series of low temperature resistant low light tolerance after purification,
It is named as distinct emerald green white No. two.
3rd, contrast test is carried out, further determines that selected germplasm
Observation is planted experimentally to judge distinct emerald green white No. two yield, adaptability, disease resistance and quality and flavor.To the self-mating system of incubation
Carry out appropriate low temperature environment greenhouse cell to repeat, early yield measures and added up total output；Using hedonic scoring system, to its quality
Do preliminary check analysis；Judge it to downy mildew, the resistance of powdery mildew by the disease index of blade.
Multispots trial demonstration is carried out, selection meets the germplasm of breeding objective requirement.
4th, result of the test
4.1 yielding ability
The strain of measurement cultivation, line-spacing, obtain every mu of strain number in comparative test greenhouse；Diagonally take at 3 points, every continuous tune
10 plants are looked into, statistics knot melon number, obtains averagely every plant knot melon number；30 commodity fruits are won per kind to weigh, and calculate average single melon
Weight, Production rate are calculated as follows：Yield (kilogram/667m2)=mono- melon weight × individual plant knot melon × mu strain number.With purified rejuvenation
Haiyang White cucumber two compare.Compared with control volume increase 33.6%, difference reaches the pole level of signifiance.
Regional testing plot area 15-30m2, 667m2Yield=(cell production/plot area) × 667.Increase production compared with control
More than 32.7%, total yield relatively compares volume increase more than 43.5%, and difference reaches the pole level of signifiance.
Pilot production uses multiple spot pilot production.Early yield relatively control volume increase more than 29.7% respectively；Total output is more right
According to volume increase more than 34.2%.
4.2 ratio of female
While variety test, regional testing and pilot production is carried out, full female plant and strong female plant are investigated.It is distinct
Emerald green white No. two complete female strain rates 95.2%, strong female strain rate 3.6%, hence it is evident that better than control Haiyang White cucumber three (0 and 0.3%).
4.3 quality
Using hedonic scoring system, 5 people participate in.Flavor quality is by pericarp thickness, quality is tender and crisp, sour-sweet degree, has free from extraneous odour etc.
Four indices, each 10 points of score, full marks 40 divide；Exterior quality investigates 5 melons, by color of the leather, melon length, long, seeds chamber footpath melon
4 marking such as transverse diameter, each 10 points of meter, full marks 40 divide.Distinct emerald green white No. two are respectively 36.8 and 39.3, hence it is evident that better than control
(Haiyang White cucumber No. two 29.5 and 30.3).
4.4 low temperature resistant and plant growing ways
Better than control, suitable Protectorate cultivation.By thick and dry to 12 degrees Celsius of germination percentages, plant height, total blade area, stem
The overall targets such as substance weight judge its cold-resistant low light tolerance index.Observation measurement finds that distinct emerald green white No. two tolerance to low temperature and weak light are more right
Significantly increased according to (Haiyang White cucumber two).
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (4)
1. a kind of selection of tolerance to low temperature and weak light Haiyang White cucumber female series, comprises the following steps：
1) Haiyang White cucumber population improvement：
1. basic population is collected：Sun Ge village White cucumber, Chu Ge village White cucumber, hair city east Fang Wu White cucumbers are selected, selfing respectively carries
After pure, colony based on reserving seed for planting；
2. miscegenation：By basic population miscegenation, the fine individual plant for possessing Haiyang White cucumber commodity is selected from the next generation；
3. individual plant systematic breeding：The fine individual plant selected to previous step uses individual plant systematic breeding method, by each individual plant each
Sowing 1 furrow turns into a family, is selfed and reserves seed for planting inside family, chooses defect individual；
2. -3. four time 4. after choosing the defect individual repeat step, the defect individual that finally obtains purifies through selfing obtains sea
Yang white cucumber self-mating system；
2) crossbreeding：
A. using foregoing Haiyang White cucumber self-mating system as female parent, hybridized using northeast dry land cucumber as male parent, obtain F1 generation kind
Son；
B. select both possessed Haiyang White cucumber biological character from F1 generation plant, while setting percentage is high again, cold-resistant individual plant 1-2
；
C. using foregoing White cucumber self-mating system as recurrent parent, northeast dry land cucumber is as nonrecurrent parent, by what is be selected in step B
F1 generation individual plant is returned with foregoing White cucumber self-mating system, and the individual plant 2-4 that low temperature resistant and dim light, setting percentage are high is selected in backcross progeny
；
D. repeat step C backcrossing adds up 4-5 generations, selects the high fine individual plant of low temperature resistant and dim light, setting percentage some, is selfed pure
Haiyang White cucumber female series of tolerance to low temperature and weak light can be obtained by changing.
2. selection as claimed in claim 1, it is characterised in that the White cucumber population improvement of step 1) Haiyang is 2. in miscegenation,
The fine individual plant for possessing Haiyang White cucumber commodity, refer to that sugar-preserved gourd grows 16~21cm, revealed the green during melon color is white, melon is short, melon
Thin skin, the crisp White cucumber of mouthfeel sweet tea.
3. application of the selection in the White cucumber crossbreeding of Haiyang described in claim 1-2 any claims.
4. application as claimed in claim 3, it is characterised in that the application is transferred to method for backcrossing and educated in the hybridization of Haiyang White cucumber
The application of kind.
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| CN111149691A (en) * | 2020-03-20 | 2020-05-15 | 山东永盛农业发展有限公司 | Method for breeding weak light-resistant inbred line by utilizing tomato hybrid |
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