CN105596705A - Application of traditional Chinese medicine composition in preparation of medicine for treating cerebral thrombosis - Google Patents

Abstract

The invention discloses an application of a traditional Chinese medicine composition in preparation of a medicine for treating cerebral thrombosis, belonging to the field of traditional Chinese medicine. The traditional Chinese medicine composition disclosed by the invention is prepared from the following raw materials: peach seeds, rhizoma seu radix notopterygii, flos sophorae, paniculate swallowwort root, radix rehmanniae preparata, lily, honeysuckle, radix salviae miltiorrhizae, fructus lycii, poria cocos, rhizoma chuanxiong, radix angelicae, berberine, radix paeoniae alba, rhodiola rosea, liquorice, fructus citri and polygala tenuifolia. The traditional Chinese medicine composition disclosed by the invention has a perfect treatment effect in treating or preventing cerebral thrombosis while few side effects are caused and the clinical popularization value is remarkable.

Description

The purposes of a kind of Chinese medicine composition in the medicine of preparation treatment cerebral thrombus

The application is the divisional application of the application for a patent for invention that proposes of applicant Cui Xinming (denomination of invention is: a kind of Chinese medicine composition for the treatment of cerebral thrombus and preparation method thereof, application number is: 2014101131861, the applying date is: on March 25th, 2014).

Technical field

The invention belongs to medical technical field, be specifically related to the purposes of a kind of Chinese medicine composition in the medicine of preparation treatment cerebral thrombus.

Background technology

Cerebrovascular disease claims again cerebral apoplexy, is to make to supply a kind of the nervous system disease due to the blood vessel generation pathology of brain blood by the various causes of disease. Its Etiological be the reasons such as cerebral artery system disease damage (as cerebral arteriovenous malformation) the cerebral artery luminal stenosis, vasopasm, the obturation that cause or break, Oligemia or block completely, brain blood circulation and dysfunction, the impaired and series of symptoms that occurs of brain tissue. Mainly comprise ischemic and hemorrhagic cerebrovascular disease. Wherein (ICVD claims again Ischemic Stroke) account for 80% left and right.

Ischemic cerebrovascular disease refers to that local brain tissue comprises that nerve cell, spongiocyte and contact fiber are due to the sex change of blood supply disorder generation, the afunction that property is crossed in necrosis or. The cerebral arteries emphraxis that Intravascular Thrombus formation, embolism, hemadostewnosis cause is the main cause of cerebral arterial thrombosis. Ischemic cause cranial nerve cell damage and dead mechanism of action various, complicated, after cerebral arterial thrombosis (being cerebral ischemia), due to the supply of brain shortage blood and oxygen, cause large brain energy metabolism unbalance, produce a series of pathologic damage, as oxidative stress, toxicity of excitatory amino acid, calcium overload, inflammatory reaction etc., thereby cause neuronic mortality. It is common disease, frequently-occurring disease clinically, and the death rate and disability rate are very high, existing one of the three universally acknowledged large lethal diseases that become. Treatment is mainly the recovery of nervous function after thrombolysis, the neuron of being at death's door of saving ischemic area (half blanking bar) and promotion damage clinically. Control ICVD is current mankind difficult medical problem in the urgent need to address. At present U.S. FDA has only been ratified tissue plasmin activation factor (tPA) for the thromboembolism treatment after apoplexy, but its therapeutic time window is very narrow, only uses just effectively in 4.5 hours in apoplexy; But also exist hemorrhage and Ischemia Reperfusion to increase the weight of the danger of brain damage. And comprise that for the neuroprotective drug of ischemic stroke treatment calcium channel blocker is if Nimodipine, glutamate receptor antagonists are if Dizocilpine (dizocilPine), antioxidant or free radical scavenger are as Edaravone, NO signal transduction pathway conditioning agent lu pei Shandong mile (Iubeluzole) and inflammation inhibitor Enlimomab (enlimomab) etc. at present. But the therapeutic action having in them is imprecise or specificity is not strong, some toxic and side effects compared with large, tolerance is little, have also in clinical before or the clinical research stage, being difficult to actively affects in the performance of control cerebral arterial thrombosis. Thereby, develop quick control brain ischemia medicament effective, safety and stability extremely urgent. Bring into play very important positive role at this field traditional Chinese medicine.

Make a general survey of the research direction of world's new drug, be mainly single chemicals and preparations thereof of research and development the eighties in the past, starts to develop biotechnology and natural medicinal plants after the eighties, and drug research direction is tending towards diversity. In conjunction with modern traditional Chinese medicine theory of development, from improving the angle of curative effect of medication, reduction side effect, through traditional Chinese medicine ingredients being screened to the preparation that extraction and/or compatibility make, its side effect is little, safe, has the advantage that chemical synthetic drug can not be compared.

Summary of the invention

In prior art, chemicals is cured the symptoms, not the disease, and easily damages gastrointestinal tract mucously, and poisonous side effect of medicine is large, and Chinese medicine exists uncertain therapeutic efficacy fixed, lacks relative medicine preparation. large in order to overcome existing cerebral thrombus chemotherapeutic agent toxic and side effect, cerebral thrombosis treatment effect is difficult to lasting prior art deficiency, the invention provides a kind of Chinese medicine composition for the treatment of cerebral thrombus, this Chinese medicine composition good effect, side effect is little, it is mainly made by following raw material: peach kernel 10-50 part, notopterygium root 10-20 part, sophora flower 5-20 part, paniculate swallowwort 5-15 part, cultivated land 5-10 part, lily 5-15 part, honeysuckle 5-15 part, red sage root 1-10 part, matrimony vine 5-15 part, Poria cocos 9-15 part, Ligusticum wallichii 9-15 part, root of Dahurain angelica 5-15 part, berberine 0.2-9 part, root of herbaceous peony 5.5-15 part, rhodiola root 6-9 part, Radix Glycyrrhizae 10-15 part.

Peach kernel has promoting blood circulation, relaxes bowel, relieving cough and asthma effect. Clinical in through closing, dysmenorrhoea ， wei lump in the abdomen lump in the abdomen, injury from falling down, the diseases such as the dry constipation of intestines. In peach kernel, be rich in number of chemical composition, be rich in amarogentin, emulsin, volatile oil, fat oil, in oil, mainly contain olein and a small amount of glyceryl linoleate. Modern pharmacology studies show that, peach kernel extract can obviously increase cerebral blood flow (CBF), increases the CBF of dog femoral artery, reduces vascular resistence, improves hemodynamic situation. Extract can improve the liver surface microcirculation of animal, and promotes choleresis. Peach kernel can make the hemorrhage and clotting time of mouse obviously extend, and decoction has inhibitory action to external thrombus, and decocting liquid has fiber facilitation. In peach kernel, contain 45% fat oil Castor Oil enteron aisle, be beneficial to defecation. Peach kernel can promote primipara's uterine contractile and hemorrhage. Water decoction and extract have analgesia, anti-inflammatory, antibacterial, anti-allergic effects. Amarogentin in peach kernel has the effect of antitussive and antiasthmatic and anti-hepatic fibrosis.

Notopterygium root is conventional Chinese medicine. Medicinal history is long, first appeared in Shennong's Herbal, lists under levisticum item. Notopterygium root bitter, pungent, warm in nature. There is loose exterior cold curing rheumatism, the effect in sharp joint. Control that lossless, the wind-cold-dampness arthralgia of catching cold, have a headache, a strong muscular contracture, joint are ached, geomantic omen edema, the swollen sore of pain etc. Modern analysis shows, notopterygium root mainly contains bornene, twists rubber alkene, the compositions such as words product enol and acetic acid rough gentian brain vinegar. Clinical in impotence and seminal emission, enuresis frequent micturition, waist knee crymodynia, suffers from a deficiency of the kidney and breathes heavily, Diarrhoea; External application curing vitiligo, alopecia areata. Induce sweat loose cold, expelling wind and eliminating dampness, the diseases such as pain relieving. Modern pharmacology research notopterygium root has significant antipyretic and anti-inflammatory and throe effect.

Sophora flower is again Chinese scholartree stamen, effect of sophora flower: cooling blood and hemostasis, clear liver and improve vision, sophora flower effect: control that blood under hematochezia, hemorrhoid, hematuria blood drench, the headache of liver heat, red eye, swell pain, sophora flower medication taboo: the careful sophora flower that takes of deficiency-cold in spleen and stomach person, sophora flower can (drugs) to be decocted and taken as a drink, sophora flower also can be equal to use with selfheal, chrysanthemum, paniculate swallowwort, to increase the effect of clearing liver-fire improving eyesight. Clinical curing mainly: hematochezia; Blood under hemorrhoid; Bloody flux; Hematuria; Blood drenches; Uterine bleeding; Spit blood; Bleeding from five sense organs or subcutaneous tissue; The headache of liver heat; Red eye, swell pain; The carbuncle sore that swells.

Paniculate swallowwort, cures mainly: analgesia, cough-relieving, inducing diuresis for removing edema, promoting blood circulation and detoxication. Treat for stomachache, toothache, rheumatalgia pain, bellyache in menstrual period, chronic bronchitis, ascites, oedema, dysentery, enteritis, traumatic injury, eczema, nettle rash, venomous snake bite. 1, for various pain diseases such as arthralgia due to wind-dampness, pain in the back, traumatic injury pain, abdominal pain, toothache. Paniculate swallowwort has good wind-expelling pain-stopping effect, is widely used for the various pain diseases due to rheumatism, cold solidifying, the stagnation of the circulation of vital energy, blood stasis. Also for postoperative pain and cancerous swelling pain, there is certain analgesic effect in recent years. Can apply by single, or with the relevant medicine of card compatibility. 2, by skin diseases such as eczema, wheal, stubborn dermatitis. This product has the effect of dispellingging wind and arresting itching. Can be alone for oral administration or decoct soup washout, also can compatibility kuh-seng, the medicine of the eliminating dampness and heat such as the fruit of summer cypress, the root bark of shaggy-fruited dittany. In addition, this product can also antidote against snake bite, and controls venomous snake bite. Can be with Chinese lobelia with using for oral administration or external application.

Cultivated land is the piece root of scrophulariaceae rehmannia glutinosa plant, has another name called prepared rhizome of rehmannia or throws oneself on the ground, and concocts and forms through processing. Cultivated land is a kind of first-class Chinese medicine, has the enriching yin of enriching blood effect, can be used for the deficiency of blood sallow, dizzy, palpitation and insomnia, irregular menstruation, the diseases such as uterine bleeding, also can be used for hectic fever hectic fever due to yin, the night sweat of the deficiency of the kidney yin, the disease such as pass out semen, quench one's thirst, and are one of asthenic symptoms class non-prescribed medicine medicine Liuwei Dihuang Wan main components. Prepared rhizome of rehmannia is first appeared in Shennong's Herbal, and clinical use has fresh, raw, ripe three kinds. All there is the merit of nourishing Yin and promoting production of body fluid, and control all cards of Yin and fluid deficiency. Fresh rehmannia root sweetness and bitterness Great Cold, though a little less than the power of enriching yin, be longer than clearing heat and cooling blood, purging intense heat relieving restlessness, is used for that blood-head is evil contains, syndrome of yin deficiency and depletion of fluid; It is slightly inferior but be longer than the moon of the kidney that nourishes heart that the sweet cold matter of raw (do) glutinous rehmannia is moistened the power of cool blood, Gu Xuereyin injure fever due to yin deficiency person suitable it; Prepared rhizome of rehmannia is sweet in flavor and warm in property, enters liver kidney and the special nourishing the blood and yin of merit, life essence-filling, marrow-benefitting, and all kidney-KIN being deficients, marrow virtual loss person, all can use it. Cultivated land is for the insufficiency of the kidney yang, the Yang-function insufficiency of kidneyzang, and aversion to cold and cold limbs, impotence and seminal emission, urine is lost drop, or the anorexia and loose stool of failure of fire to generate earth, the product adapted of many and monkshood, Chinese cassia tree isothermal sun, this i.e. the meaning of so-called " be apt to yang-tonifying person, must in treating YANG within YIN ". With controlling, cloudy subcutaneous ulcer is cold solidifying, this product normal with the adapted such as deer horn glue, Chinese cassia tree, Chinese ephedra with channel warming and blood nourishing, loose trembling with fear promoted blood circulation.

The traditional Chinese medical science think lily have moisten the lung and relieve the cough, effect, the especially fresh lily bulb of clearing away the heart fire and tranquillizing be more sweet delicious. The people that lily is particularly suitable for foster lung, nourishing the stomach eats, such as the patient of chronic cough, pulmonary tuberculosis, aphthae, dry, halitosis. Lily not only has good nutritious tonifying effect, and for the patient such as the chronic diseases marked by deficiency of vital energy, tuberculosis, neurosis is of great advantage after being ill, various cancers is had to good curative effect. Aspect the prevention and treatment of tumour, lily is used for treating lung cancer, nasopharyngeal carcinoma, cutaneum carcinoma etc. When the symptoms such as, dry weak in the body void that above-mentioned cancer is carried out occurring after radiotherapy, vexed, dry cough phlegm, cook congee together with polished rice with fresh lily bulb, after calling in again appropriate rock sugar or honey, eat, to building up health, inhibition cancer cell growth, alleviate the reaction of radiotherapy, there is good effect. For another example, fresh lily bulb and white sugar are smash to deposited affected part in right amount altogether,, infiltration person hemorrhage to cutaneum carcinoma ulceration also has certain therapeutic action.

Honeysuckle is cold in nature, and taste is sweet, enters lung, the heart, stomach warp, has clearing heat and detoxicating, anti-inflammatory, qi-restoratives and treat effect of wind, cures mainly disease under turgor, warm disease heating, the diseases such as hot malicious large carbuncle and tumour. The illnesss such as it is dizzy for giddy, dry is done yearningly, hidrosis is unhappy, enterogastritis, bacillary dysentery, measles, pneumonia, encephalitis, epidemic meningitis, acute mastitis, septicemia, appendicitis, skin infection, ulcer furunculosis, erysipelas, mumps, suppurative tonsillitis all have certain curative effect. Lonicera flower tea has unique weight losing function simultaneously, can also suppress and the pathogen of killing bottleneck throat, and old man and children are had to anti-infective effect. So, often take honeysuckle immersion or decoction and be conducive to wind fire hot eyes, abscess of throat, obesity, liver heat symptom-complex and the hypertensive treatment of liver pattern of fever and rehabilitation.

The red sage root has another name called red ginseng, radix salviae miltiorrhizae, red etc. For dicotyledon Labiatae, dry root and rhizome. There is promoting blood circulation for regulating menstruation, stasis-dispelling and pain-killing, the cool blood carbuncle that disappears, the relieving restlessness that clears away heart-fire, effect of nourishing blood and tranquilization. In modern pharmacology research, the red sage root is more for Strategies of Anti-fibrosis Therapy research. Its mechanism may be: 1. improve microcirculation in liver, protect liver cell, alleviate degeneration of liver cells necrosis; 2. strengthen the phagocytosis of reticuloendothelial cell; 3. strengthen OA, improve fibrin and connect level, alleviate hepatocellular injury; 4. suppress MDA, reduce oxygen radical to hepatocellular damage. Above mechanism can be protected liver cell, reduces the stimulation to sternzellen such as hepatocellular injury, necrosis, suppresses startup and the development of liver fibrosis; In liver, collagen content obviously reduces, and urine HPP discharge rate raises, and points out this medicine can promote established collagen degradation, and the collagen of hyperplasia is hydrolyzed absorption again.

Matrimony vine (formal name used at school: Lyciumchinense) is the multi-branched shrub plant of Solanaceae Lycium, and high 0.5-1 rice, can reach more than 2 meters when cultivation. All there is distribution both at home and abroad. Matrimony vine whole body is precious, and bright Li Shizhen (1518-1593 A.D.) Compendium of Material Medica is recorded: " spring is adopted folium lycii, name day essence grass; Summer picks flowers, name houseleek; Qiu Caizi, the name fruit of Chinese wolfberry; Dong Caigen, the name root bark of Chinese wolf-berry ". Matrimony vine tender leaf also claims matrimony vine head, edible or make Chinese wolfberry tea. Modern study, the fruit of Chinese wolfberry has the blood sugar of reduction, lipotropy, and can antiatherogenic effect.

Poria cocos, is commonly called as Yun Ling, Song Ling, Fu spirit, and for colonizing in the fungi plant on pine roots, shape is as sweet potato, crust pitchy, the inside white or pink. Ancients claim that Poria cocos is " god medicine " at 4 o'clock, because its effect is very extensive, regardless of the four seasons, by it and various compatibility of drugs, no matter cold, temperature, wind, all diseases that wets can be brought into play its unique effects. Poria cocos taste is sweet, light, property is flat, be used as medicine there is clearing damp and promoting diuresis, the function of beneficial spleen and stomach, antitoxic heart-soothing and sedative. Modern medicine study: Poria cocos energy enhanced machine body immunity function, pachymaran has significantly antitumor and protect the liver dirty effect.

The pungent temperature of Ligusticum wallichii is fragrant dry, walks and does not keep, can go loose, the up top, mountain peak that reaches; Enter again blood system, the descending sea of blood that reaches. Promoting blood circulation effect is extensive, the various illnesss of suitable stagnation of blood stasis; Wind-expelling pain-stopping, effectiveness is excellent, can control the diseases such as a wind headache, arthralgia due to wind-dampness. It is the gas medicine in blood that former times people calls Ligusticum wallichii, nearly says the functions such as pungent loose, the Xie Yu of its residence, sensible, pain relieving. The pungent temperature rise of this product is loose, and all hyperactivities of yang due to yin deficiency and liver-yang hyperactivity person should not apply; Menorrhalgia, pregnant woman also avoid use.

The root of Dahurain angelica, warm in nature, taste is pungent micro-sweet, has and dispels rheumatism; Logical surreptitiously pain relieving; Effect of detumescence and apocenosis. Main cold headache; Pain in the supra-orbital bone; Toothache; Nasal obstruction; Nasosinusitis; Wet victory is rushed down for a long time; Women leukorrhea; Ulcer sores; Venomous snake bite. Clinical in cold headache, pain in the supra-orbital bone, nasal obstruction, nasosinusitis, toothache, leukorrhea, sore swells and ache. Also can be used for inducing sweat loose cold, wind-expelling pain-stopping, clearing the nasal passage, eliminating dampness is band only, detumescence and apocenosis. Control headache, pain in the supra-orbital bone, dentalgia, nasosinusitis, cold-dampness stomachache, intestines wind anal fistula, leukorrhea with reddish discharge, ulcer sores, skin is dry itches, mange. Rhizoma atractylodis have drying damp and strengthening spleen, expelling wind and clearing away cold, and effect of improving eyesight, for dampness taste, abdominal fullness and distention, cold-dampness leukorrhea, damp-warm diseases and damp invasion of lower energizer, pin swelling and pain in knee, impotence are soft unable. Control dampness taste, and see the thick disease such as turbid of abdominal fullness and distention, poor appetite, lassitude hypodynamia, white and greasy fur, the compatibilities such as normal and the bark of official magnolia, dried orange peel are applied.

Berberine is used for the treatment of diarrhoea as non-prescribed medicine in clinical always, modern pharmacology there is significant heart failure resistance, anti-arrhythmia, reduction cholesterol, anti-vascular smooth muscle cell proliferation processed, improve the effects such as insulin resistance, antiplatelet, anti-inflammatory but studies confirm that berberine, thereby unify aspect the nervous system disease and may have extensive, important application prospect at cardiovascular system, day by day come into one's own. Berberine is a kind of important alkaloid, is that China applies Chinese medicine for a long time. Can from the plants such as the coptis, golden cypress, barberry, extract. It has significant bacteriostasis. Conventional Halomine is again Berberine hydrochloride. Berberine energy enantiopathy pathogenic microorganism, various bacteria is had to inhibitory action as shigella dysenteriae, tubercle bacillus, pneumococcus, typhoid bacillus and corynebacterium diphtheriae etc., wherein the strongest to shigella dysenteriae effect, be commonly used to treat the disease of digestive tracts such as bacillary gastroenteritis, dysentery. Clinically be mainly used in treating bacillary dysentery and enterogastritis, it is without the resistance to the action of a drug and side effect.

The root of herbaceous peony is the famous traditional parts of generic medicinal plants of China, and applicating history is long, and Chinese herbaceous peony begins to be loaded in Eastern Han Dynasty's Shennong's Herbal, classifies middle product as, and note has " main perverse trend, root of herbaceous peony stomachache, removes blood-arthralgia, broken hard long-pending, fever and chills hernia lump in the abdomen, pain relieving, diuresis, beneficial gas ". Nourishing blood and liver, slow middle pain relieving, astringe yin is received sweat. Control chest abdomen pain over the hypochondriac region, rush down dysentery stomachache, spontaneous sweating, fever due to yin deficiency, irregular menstruation, uterine bleeding, is with inferior disease. Modern pharmacology studies show that, the intestine in vitro of Paeoniflorin to cavy, rat and in stomach motion, and rat uterus smooth muscle all shows inhibition, and can the caused contraction of antagonism oxytocins; With acetic acid injection in mouse peritoneal, the index using writhing response as pain, Paeoniflorin has significant analgesic effect; The caused convulsions of medicinal extract energy antagonism strychnine of Chinese herbaceous peony; Root of herbaceous peony decoction has bacteriostasis to Shigella shigae in vitro, and preserved material also has inhibitory action to some pathogenic fungus. Paeoniflorin has cooling effect to small white mouse normal body temperature, and the mouse of artificial fever is also had to refrigeration function. Rat experiment metapedes sole of the foot edema is had to antiinflammatory action; Paeoniflorin has inhibitory action to the rat platelet aggregation of adenosine diphosphate (ADP) induction in vitro or when intravenous injection; Paeoniflorin has obvious inhibitory action to irritate the digestive tract in rats ulcer of bringing out due to anxiety.

Rhodiola root is dry root and the rhizome of crassulaceae plants rhodiola, and effect of rhodiola root and effect are energy kidney tonifying, regulate the flow of vital energy and nourish blood, and have promoting blood circulation and hemostasis, removing heat from the lung to relieve cough, antipyretic, and stop the effect under band, and the side effect of rhodiola root is not temporarily found. Its nature and flavor are sweet, and hardship is flat, returns lung, the heart channel of Hang-Shaoyin, has qi and activate blood circulation, and the effect of relievining asthma of promoting blood circulation, cures mainly for qi deficiency to blood stasis, chest impediment and cardialgia, hemiplegia, burnout asthma. " modern practical book on Chinese herbal medicine " says that its effect has seven, " one, central inhibitory action. Two, antifatigue effect. Three, cardiotonic. Four, antiinflammatory action. Five, suppress blood sugar rising effect. Six, anti-peroxidation effect. Seven, resisting microwave radiation effect ".

Chinese medicine composition of the present invention taking except long-pending numbness, dispersing wind and cold, wines used as antirheumatic, strengthening the bones and muscles are as main standard, has significant anti-inflammatory after Chinese medicine composition combination of the present invention, eases pain and activate blood circulation and disperse blood clots effect on traditional Chinese medicinal components is selected. The present invention has carried out preferably the parts by weight of above-mentioned all taste Chinese medicine, and preferred condition is the enhancing of medicine to cerebral thrombosis treatment effect after Chinese and Western medicine compatibility uses. As a preferred embodiment of the present invention, Chinese medicine composition of the present invention is mainly made by the raw material of following weight portion: 30 parts, peach kernel, 15 parts of notopterygium roots, 15 parts, sophora flower, 10 parts of paniculate swallowworts, 8 parts, cultivated land, 10 parts of lilies, 10 parts of honeysuckles, 6 parts of the reds sage root, 10 parts of matrimony vines, 12 parts, Poria cocos, 12 parts of Ligusticum wallichiis, 10 parts of the roots of Dahurain angelica, 5.5 parts of berberines, 10 parts of the root of herbaceous peonys, 8 parts of rhodiola roots, 12 parts, Radix Glycyrrhizae.

Preferably, Chinese medicine composition of the present invention also further contains 5 parts of citrons, 9 parts of polygala roots. Citron has another name called citron, is Rutaceae citrus plant, and pungent, the micro-hardship of citron taste, acid are warm in nature; Return liver, lung, the spleen channel; The fragrant row of gas is loose, can rise and can fall; Have dispersing stagnated hepatoqi, wide chest reduces phlegm, dehumidifying and in effect, cure mainly distending pain in the chest and hypochondrium, coughing with a lot of sputum, gastral cavity abdomen ruffian pain, eat stagnant vomit contrary, oedema beriberi. Polygala root is conventional Chinese medicine, be recorded in the earliest Shennong's Herbal, classify as top grade, and be regarded as support life key medicine, warm in nature, bitter, pungent, the function of there is the intelligence development of calming the nerves, eliminate the phlegm, subsiding a swelling, the insomnia and dreamful sleep causing for breakdown of the normal physiological coordination between the heart and the kidney, forgetful palpitation with fear, wandering, expectoration is not well, sore swollen toxin, mastosis.

In order to express better Chinese medicine composition of the present invention, Chinese medicine composition of the present invention can be prepared into conventional clinically formulation. Such as, the preparations such as powderous preparations, powder, pill, sublimed preparation, paste, granule, oral liquid, syrup, tablet, capsule. Preferably, Chinese medicine composition of the present invention is prepared into powder, aqua, tablet or capsule according to conventional preparation technology.

The invention provides a kind of preparation method of Chinese medicine composition described above, it mainly comprises following step: get the above-mentioned Chinese medicine of recipe quantity and be broken into meal, doubly adding volumetric concentration according to the 4-9 of meal gross weight is the ethanolic solution of 40%-95%, refluxing extraction three times, return time is 2-5h, filters, filtrate recycling ethanol, cold filtration, washes with water, after being dried, obtains Chinese medical concrete; After being made to dried cream powder, its pulverizing add the berberine of recipe quantity and the red sage root can obtain Chinese medicine composition of the present invention. Those skilled in the art can be at the technical conventional Chinese medicine pharmaceutical dosage form clinically for preparing of this preparation method, as tablet, capsule etc.

The present invention also asks to protect the purposes of above-mentioned Chinese medicine composition in preparation treatment cerebral thrombus medicine. Pharmaceutical composition of the present invention when cerebral thrombus, demonstrates significant anti-inflammatory and antalgic in treatment, activity activates blood circulation and disperses blood clots. Drug effect embodiment 19 of the present invention shows, with model group comparison, the low middle Senior Three of a Chinese medicine composition of the present invention dosage group all can significantly reduce dimethylbenzene induced mice ear swelling value (P< 0.01), show that Chinese medicine composition of the present invention has the effect of the mice ear of remarkable inhibition dimethylbenzene induction, and there is significant dose-effect relationship in its reduction effect to ear swelling. Under same experiment condition, positive control drug XUESHUANTONG JIAONANG group also significantly reduce ear swelling value (P< 0.05), but its antiphlogistic effects is obviously not as good as Chinese medicine composition of the present invention. Drug effect embodiment 20 of the present invention shows, model group and normal control comparison, the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity all have significantly and increase (P< 0.05). With model group comparison, basic, normal, high each dosage group of the Chinese medicine composition of the present invention all remarkable or utmost point has reduced the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity significantly, reduction amplitude amount effect relationship, wherein high dose there were significant differences (P< 0.05). Under same experiment condition, the glad safe sumalin sheet group in positive control drug sky has also reduced the high cut index of whole blood, the low cut index of whole blood, erythrocyte aggregation index, but it improves effect all not as good as Chinese medicine composition of the present invention to Hemorheology index.

In a word, pharmaceutical composition of the present invention, aspect treatment cerebral thrombus, compared with prior art has following advantage:

1) compared with the glad safe sumalin sheet in positive control drug sky or XUESHUANTONG JIAONANG, pharmaceutical composition Chinese traditional medicine composition of the present invention is not only more remarkable at anti-inflammatory and ease pain, and aspect activating blood circulation and dispersing blood clots, be also significantly better than positive drug, this shows that the present composition has significant synergy in cerebral thrombosis treatment, not only can significantly improve the symptom of cerebral thrombus, can also delay the development of cerebral thrombus, contribute to cerebral thrombus patient's recovery from illness process;

2) compared with the chemotherapeutic agent of current treatment cerebral thrombus, Chinese medicine composition of the present invention is natural pure Chinese medicinal preparation, bad reaction and side effect significantly reduce, particularly it does not produce any destruction for intestinal mucosa, and Chinese medicine composition effect of the present invention is comprehensive, medication effect is better, has significantly improved cerebral thrombus patient's compliance, and has improved patient's quality of life;

3) existing antibiotic medicine cerebral thrombus initial stage result for the treatment of still can, but along with obvious drug resistance problem all appears in the prolongation for the treatment of time, its result for the treatment of for cerebral thrombus declines; In Chinese medicine composition of the present invention, contain multi-medicament component, action target spot is numerous, has effectively solved the Commpensation And Adaptation of cerebral thrombosis treatment medicine, its to the result for the treatment of of cerebral thrombus not because for the treatment of time extend decline.

Detailed description of the invention

Further describe the present invention by specific embodiment below, the present invention is not limited only to following examples. Within the scope of the invention or not departing from content of the present invention, spirit and scope, change, combination or replacement that the present invention is carried out, be apparent for a person skilled in the art, and be included within the scope of the present invention.

Part I Chinese medicinal composition preparation of the present invention, preparation and using method thereof

Embodiment 1-6 Chinese traditional medicine composition composition powders

Table 1 Chinese traditional medicine composition composition powders prescription of the present invention

Embodiment 1 preparation method: get each medicinal material in composition, grind to form powdery according to common process, cross 100 mesh sieves, little fire drying is for subsequent use, adds the berberine of recipe quantity and the red sage root to obtain powder. When use, water is directly taken after mixing it with water after dissolving. Embodiment 2-6 preparation method is with embodiment 1.

Embodiment 7-12 Chinese medicine composition aqua

Table 2 Chinese medicine composition aqua prescription of the present invention

Embodiment 7 preparation methods: get each medicinal material in prescription, according to conventional decocting for Chinese herbal medicine technological operation, add berberine and the red sage root of recipe quantity after the filtering dregs of a decoction and get final product. This aqua (or claim decoction) is during for cerebral thrombosis treatment, and take 2-3 every day. Embodiment 8-12 preparation method and using method are with embodiment 7.

Embodiment 13-18 Chinese medicine composition tablet/capsule of the present invention

Table 3 Chinese medicine composition tablet of the present invention/capsule prescription

Embodiment 13 preparation technologies: each traditional Chinese medicine powder of getting recipe quantity is broken into meal, adding volumetric concentration according to 5 times of meal gross weight is 80% ethanolic solution, refluxing extraction three times, the each 3h of return time, filters filtrate recycling ethanol, cold filtration, washes with water, after being dried, obtains Chinese medical concrete; Chinese medical concrete drying, pulverizing are made to dried cream powder, in dried cream powder, add berberine and the red sage root of recipe quantity, then add conventional adjunct ingredient to utilize prior art to be prepared into tablet or capsule.

Embodiment 14-18 preparation technology is with embodiment 13.

Part II Chinese medicine composition pharmacodynamic study of the present invention

Embodiment 19 Chinese medicine composition paraxylene of the present invention causes the impact of mice ear

Get 72 of healthy ICR mouse, ♀ ♂ half and half, divide 6 groups, 12/group, be model group, day glad safe sumalin sheet (Hainan Co., Ltd of San Ye pharmaceutical factory, lower same), XUESHUANTONG JIAONANG group (Haikou Kangliyuan Pharmaceutical Co., Ltd), Chinese medicine composition of the present invention (making by Chinese medicine composition prescription and preparation technology described in embodiment 1) low dose group, middle dosage group and high dose group. 1 time/d of every ig administration; Administration 14d, 60min after last medicine, mouse right ear is evenly smeared dimethylbenzene 0.03mL/ and is only caused swollenly, cuts ears and beat auricle and weigh after 2h, calculates ear swelling value (mg). Swelling value=auris dextra weight-left ear weight.

Result show, with model group comparison, the low middle Senior Three of a Chinese medicine composition of the present invention dosage group all can significantly reduce dimethylbenzene induced mice ear swelling value (P< 0.01), show that Chinese medicine composition of the present invention has the effect of the mice ear of remarkable inhibition dimethylbenzene induction, and there is significant dose-effect relationship in its reduction effect to ear swelling. Under same experiment condition, positive control drug XUESHUANTONG JIAONANG group also significantly reduce ear swelling value (P< 0.05), but its antiphlogistic effects is obviously not as good as Chinese medicine composition of the present invention.

The impact of table 4 Chinese medicine composition paraxylene of the present invention induced mice ear swelling ( ）

Note: with model group comparison, *P＜0.05，**P＜0.01。

The improvement effect of embodiment 20 Chinese medicine composition of the present invention to rat blood stasis models

Get healthy SD rat, body weight 180-200g, 70, male and female half and half, be divided at random 7 groups by body weight: basic, normal, high group of Normal group, model group, day glad safe sumalin sheet group, XUESHUANTONG JIAONANG group and Chinese medicine composition of the present invention (making by Chinese medicine composition prescription and preparation technology described in embodiment 1), 10 every group. Gastric infusion, successive administration 14 days, Normal group and model group are to 0.5%CMC, and all the other give corresponding medicine, and dosage is as shown in table 4. Administration the 13rd day, 14 days, except Normal group, all the other treated animals are pressed 1mg/Kg hypodermic injection adrenalin hydrochloride, and 2 times/day, interval 4h, after injection, 2h puts animal in 4 DEG C of frozen water 15min for the first time. Administration is got 4-5ml blood anticoagulant heparin on the 15th day and is measured blood rheology parameter, gets the liver conversion organ coefficient of weighing.

Result shows, model group and normal control comparison, the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity all have significantly and increase (P< 0.05). With model group comparison, basic, normal, high each dosage group of the Chinese medicine composition of the present invention all remarkable or utmost point has reduced the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity significantly, reduction amplitude amount effect relationship, wherein high dose there were significant differences (P< 0.05). Under same experiment condition, the glad safe sumalin sheet group in positive control drug sky and XUESHUANTONG JIAONANG group have also reduced the high cut index of whole blood, the low cut index of whole blood, erythrocyte aggregation index, but it improves effect all not as good as Chinese medicine composition of the present invention to Hemorheology index. The result statistics of liver index shows, model group and normal control comparison, and liver index significantly increases. And with model group comparison, basic, normal, high each dosage group of Chinese medicine composition of the present invention significantly reduced liver index (P< 0.01), the glad safe sumalin sheet in positive control drug sky and Chinese medicine composition of the present invention Chinese medicine composition high dose group of the present invention can reduce significantly liver index (P< 0.05), but effect is not as good as Chinese medicine composition of the present invention.

Table 5 Chinese medicine composition of the present invention on rat blood stasis models blood flow become impact ( ）

Note: with normal control comparison, P＜0.05，##P< 0.01; With model group comparison, *P＜0.05，**P＜0.01。

Experimental example 21: Chinese medicine composition of the present invention on RHRSP cerebral thrombus after the impact of blood-brain barrier (BBB) permeability

1 test method

2.1.1 the preparation of animal model:

Get the two narrow arteria renalis of kidney double fastener method of male SD rat of 2-3 monthly age, body weight (100 ± 20) g and set up RHRSP model: rat abdominal cavity anesthesia, through the long otch of the about 5cm of xiphoid-process Ventral Midline stringer, cut skin, abdominal muscle, blunt separation bilateral renal arteries, the annular silver brain clip that is 0.3mm with internal diameter is clamped respectively bilateral renal arteries initial part. Before operation, measuring blood pressure once, is surveyed once weekly continuous 12 weeks after operation. Separately get 10 SD rats that body weight is suitable, survey its blood pressure as normal control. Reject: in operation and postoperative death, 12 weeks after operation blood pressure is lower than 160mmHg rat, have softening kitchen range that palsy symptom and sign, dissection be shown in that left basal ganglia region is little and the rat of subarachnoid hemorrhage. Blood pressure >=160mmHg and be successful model without the RHRSP of cerebral apoplexy symptom after 16 weeks. Cause middle cerebral artery occlusion (MCAO) model to cause arteria cerebri media (MCA) thrombosis with photochemical method the RHRSP being successfully prepared: 2% yellow Jackets by 40mg/kg body weight intraperitoneal injection of anesthesia after, through left temporo side approach, at zygomatic arch and 1mm place, front lower place, aquamous part of temporal bone junction, bore by dental burr the bone window that a diameter is 5mm, can know and see left MCA trunk and branch thereof through endocranium. Dosage injection 2.5% tiger of pressing 40mg/kg body weight through femoral vein is red, green laser beam (the spot diameter 2mm of the 532nm occurring by YAG laser after 5min, intensity of illumination 0.37W) prolonged exposure MCA trunk 15min, visible this MCA far-end and each branch blood flow interrupt, and under surgical operation microscope, see that local white thrombus points out this MCA thrombosis, the success of MCAO model copy. In art, rat anus temperature remains on (37 ± 0.3) DEG C. Local wound uses penicillin dilution to rinse before sewing up, and postoperative conventional intramuscular injection penicillin 80,000 U/ only. Reject in art and postoperative 1d death and hemorrhage many or surgical operation microscope under the not clear and definite blood supply rat of whether interrupting.

Divide 6 groups at random by animal: sham-operation group, model control group, the high, medium and low dosage group of Chinese medicine composition of the present invention, Nimodipine group. The sham-operation group window that only opens seam, does not make Ear Mucosa Treated by He Ne Laser Irradiation; 4.5h after model surgery success, each group tail vein injection Chinese medicine composition 40mg/kg of the present invention, 20mg/kg, 10mg/kg respectively, Nimodipine 20mg/kg; The physiological saline of sham-operation group and model group tail vein injection same volume. Each group rat is put to death and gets brain tissue sample in MCAO postoperative (after being cerebral thrombus ischemic) 6h, 12h, 24h in batches respectively, and in put to death before 1h tail vein injection 2% Evans blue (EvansBlue, EB) normal saline solution 2ml/kg body weight, after fully circulating, dark numb animal, cut open chest through heart perfusion normal saline flushing blood vessel inner dye, broken end is got brain.

Randomly draw the brain tissue of 3 rats for each group, frozen section immediately, the crown section of the thick about 30um of row continuously, with 70% glycerine PBS buffer solution (pH8.5-9.0) mounting, under Bio-RadRadiance2100 laser scanning co-focusing microscope, observe the situation of oozing out of EB in brain tissue, observe BBB open area and degree. While observation, section is put 4 DEG C of refrigerators and is kept in Dark Place. Each group is separately got the brain tissue of 5 rats at random, weighs brain weight in wet base after blotting surface moisture; Brain tissue is placed in to homogenizer, adds 50% trichloroacetic acid to make tissue homogenate, more than moving into test tube sealing and standing 60min; The centrifugal 10min of 3000rpm/min, gets supernatant, and sepectrophotofluorometer is surveyed fluorescent value: excitation wavelength 620nm, emission wavelength 680nm; Calculate EB content in supernatant by calibration curve, then calculate every Borneo camphor and organize EB content, react each rat BBB permeability with this.

2 experimental results

2.1 laser scanning co-focusing microscope observed results

EB is bright-coloured bright red fluorescence under exciting light state. Under laser microscope, observe each treated animal brain tissue WuBBBNao district (as pineal body, area postrema and pituitary) and present homogeneous, diffusivity EB dip-dye, sharpness of border, the line sample profile that meninx red color visible fluorescence is sketched the contours of. And BBBNao district, the equal visible light spot of other each group brain tissues except sham-operation group, and be mainly distributed in thalamus, hypothalamus, cerebellum, hippocampus etc. and locate, spot size differs, and fluorescence intensity weakens to periphery gradually from the center of hot spot, obscurity boundary. The hot spot number of each group and distribution are obviously different: model group hot spot is maximum, approximately has the hot spot of 120 left and right diameter 100-200um, and intensive place in the form of sheets. Each Chinese medicine composition medicine group number of spots of the present invention significantly reduces compared with model group, and the comparison of each dosage group, presents the remarkable dose-difference opposite sex; Wherein more with low dose group hot spot, on same level, hot spot approximately has 50, but is fused into the less of sheet; Middle dosage group hot spot is less, is dispersed in distribution; High dose group hot spot is seldom dispersed in, and fluorescence is very weak.

The permeability result of 2.2 EB of rat cerebral tissue content detection blood-brain barriers

Ge Zu rat cerebral tissue sample calculates EB content by the fluorescent value recording, and data statistic analysis is in table 6.

Table 6 Chinese medicine composition of the present invention on RHRSP cerebral thrombus after the impact of blood-brain barrier permeability

Note: with the comparison of sham-operation group, ##P < 0.01; With model group comparison, * P < 0.01, * * P < 0.01.

2.3 conclusion

EB is water-soluble dye, enters after blood and can absolutely be combined with albumin rapidly, can not see through under normal circumstances blood-brain barrier. From 2.1 and 2.2 results, sham-operation ZuBBBNao district, has no EB under brain tissue microscope and oozes out hot spot; And under model group rat cerebral tissue microscope, have a large amount of EB to ooze out hot spot, and brain tissue sepectrophotofluorometer detects the EB of high-load, compared with sham-operation group, there were significant differences (P < 0.01), show cerebral thrombus tissues following MCAO in rats blood-brain barrier disruption, permeability increases, and EB albumin-binding can enter brain tissue by open BBB. Experimental result shows under Chinese medicine composition of the present invention each dosage group rat cerebral tissue microscope that EB hot spot number and brain tissue EB detect content and obviously reduce; more all there were significant differences (P < 0.01) with model group; point out Chinese medicine composition of the present invention to increase inhibited to blood-brain barrier permeability following brain injury; can protect blood-brain barrier, and the further damaged of protection brain tissue.

Claims (2)

1. the purposes of Chinese medicine composition in preparation treatment cerebral thrombus medicine, is characterized in that described Chinese medicine composition is made by the raw material of following weight portion: 30 parts, peach kernel, 15 parts of notopterygium roots, 15 parts, sophora flower, 10 parts of paniculate swallowworts, 8 parts, cultivated land, 10 parts of lilies, 10 parts of honeysuckles, 6 parts of the reds sage root, 10 parts of matrimony vines, 12 parts, Poria cocos, 12 parts of Ligusticum wallichiis, 10 parts of the roots of Dahurain angelica, 5.5 parts of berberines, 10 parts of the root of herbaceous peonys, 8 parts of rhodiola roots, 12 parts, Radix Glycyrrhizae, 9 parts of 5 parts of citrons and polygala roots.

2. the purposes of Chinese medicine composition as claimed in claim 1 in preparation treatment cerebral thrombus medicine, is characterized in that: described Chinese medicine composition is powder, aqua, tablet or capsule.