CN105586033A - 一种含有谷氨酸结构的罗丹明pH荧光探针及其应用 - Google Patents
一种含有谷氨酸结构的罗丹明pH荧光探针及其应用 Download PDFInfo
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- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 239000007850 fluorescent dye Substances 0.000 title claims abstract description 34
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 title claims abstract description 17
- 239000003269 fluorescent indicator Substances 0.000 claims abstract description 17
- 238000012544 monitoring process Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 48
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 27
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 21
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- 238000001816 cooling Methods 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- 239000012044 organic layer Substances 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 claims description 12
- -1 amino acid compounds Chemical class 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 9
- YEJSPQZHMWGIGP-YFKPBYRVSA-N L-glutamic acid, dimethyl ester Chemical compound COC(=O)CC[C@H](N)C(=O)OC YEJSPQZHMWGIGP-YFKPBYRVSA-N 0.000 claims description 7
- YEJSPQZHMWGIGP-UHFFFAOYSA-N dl-glutamic acid dimethyl ester Natural products COC(=O)CCC(N)C(=O)OC YEJSPQZHMWGIGP-UHFFFAOYSA-N 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 235000001014 amino acid Nutrition 0.000 claims description 6
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 4
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 claims description 3
- MUSLHCJRTRQOSP-UHFFFAOYSA-N rhodamine 101 Chemical compound [O-]C(=O)C1=CC=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MUSLHCJRTRQOSP-UHFFFAOYSA-N 0.000 claims description 3
- MYIOYATURDILJN-UHFFFAOYSA-N rhodamine 110 Chemical compound [Cl-].C=12C=CC(N)=CC2=[O+]C2=CC(N)=CC=C2C=1C1=CC=CC=C1C(O)=O MYIOYATURDILJN-UHFFFAOYSA-N 0.000 claims description 3
- 229940043267 rhodamine b Drugs 0.000 claims description 3
- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 claims description 3
- 239000012295 chemical reaction liquid Substances 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-L glutamate group Chemical group N[C@@H](CCC(=O)[O-])C(=O)[O-] WHUUTDBJXJRKMK-VKHMYHEASA-L 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 3
- 239000008280 blood Substances 0.000 abstract description 3
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- 239000010839 body fluid Substances 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 239000007793 ph indicator Substances 0.000 abstract 1
- 230000001850 reproductive effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 28
- 238000002189 fluorescence spectrum Methods 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 10
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 10
- 239000002994 raw material Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 230000003595 spectral effect Effects 0.000 description 6
- NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical compound CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 description 5
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 5
- 235000013922 glutamic acid Nutrition 0.000 description 5
- 239000004220 glutamic acid Substances 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 229910019213 POCl3 Inorganic materials 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- RLOWWWKZYUNIDI-UHFFFAOYSA-N phosphinic chloride Chemical compound ClP=O RLOWWWKZYUNIDI-UHFFFAOYSA-N 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
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Abstract
本发明提供了一种含有谷氨酸结构的罗丹明<b>pH</b>荧光探针,即<b>Rh-pH</b>荧光探针,所述的<b>Rh-pH</b>荧光探针的结构通式如<b>(</b><b>I)</b>所示。本发明的含有氨基酸结构的罗丹明pH荧光指示剂,该pH指示剂因具有快速响应、高灵敏度。在pH?3.2-pH8.5范围内呈现出较宽的pH响应。用于在一个密封容器中进行血液、体液等细菌培养的过程实时监测,从而及时反映细菌的繁殖参数变化,为临床提供医疗数据支撑。
Description
技术领域
本发明涉及一种含谷氨酸结构的罗丹明pH荧光探针及其应用。
背景技术
在微生物细胞生长领域,pH值是一个重要的指示参数。细胞在生长的过程中会排出大量的二氧化碳,例如:在一个密封的容器里对带有细菌的血液、痰液、体液培养过程中,其pH也会出现波动。借助于荧光指示剂高度敏的特性测量生长过程中pH的变化值,可以实时掌握细菌生长的整个过程。
理想的pH荧光指示剂应该对pH值的变化范围具有较宽的pH响应范围,即具有较宽的响应窗口。目前国内pH荧光指示剂,pH值范围大概都在(6.8-7.4)和pH值(4.5-6.0)之间。而具有较大范围的pH(3-8)荧光指示剂却很少,因此需要一种具有高灵敏度、高选择性及具有较宽的pH响应窗口的荧光指示剂。
目前国内和国际上有关罗丹明类的PH荧光探针和荧光指示剂种类繁多。而用于监测微生物培养过程的却比较少。
发明内容
本发明的目的是克服现有技术的不足之处,提供一种含有谷氨酸结构的罗丹明pH荧光探针及其应用。
本发明的含有谷氨酸结构的罗丹明pH荧光探针,即Rh-pH荧光探针,其特征在于,所述的Rh-pH荧光探针的结构通式如(I)所示:
其中,
R1=R2=R3=R4=H;
或R1=R4=H,R2=—CH2CH3,R3=—CH3;
或R1=R2=—CH3,R3=R4=H;
或R1=R2=—CH2CH3,R3=R4=H;
或R1=R2=R3=R4=—(CH2)3—。
所述的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
1)将罗丹明类化合物与三氯氧磷加入到乙腈中,保温反应3-5小时,冷却至室温后,加入氨基酸类化合物的乙腈溶液和三乙胺的乙腈溶液,室温下反应3-5小时,反应液用乙酸乙酯和水萃取,取有机层,干燥,浓缩,降温,析晶,得到白色固体;
2)将该白色固体溶于水中,加入氢氧化钠溶液,加热至50-60℃,反应过夜,降至室温,加入稀盐酸溶液,搅拌,再加入二氯甲烷溶液萃取,取有机层,干燥,浓缩,得到目标产物Rh-pH荧光探针。
所述罗丹明类化合物为罗丹明110、罗丹明6G、四甲基罗丹明TMR、罗丹明B或罗丹明101,所述氨基酸类化合物为谷氨酸二甲酯。
所述罗丹明类化合物、三氯氧磷、氨基酸类化合物与三乙胺的摩尔比为1:(3-6):1:(3-6)。
本发明的含有谷氨酸结构的罗丹明pH荧光探针作为荧光指示剂在监测微生物培养过程中pH值变化的应用。
本发明的含有氨基酸结构的罗丹明pH荧光指示剂,该pH指示剂因具有快速响应、高灵敏度。在pH3.2-pH8.5范围内呈现出较宽的pH响应。用于在一个密封容器中进行血液、体液等细菌培养的过程实时监测,从而及时反映细菌的繁殖参数变化,为临床提供医疗数据支撑。
附图说明
图1是实施例1制备的罗丹明pH荧光探针对pH变化的荧光光谱扫描图。
图2是实施例2制备的罗丹明pH荧光探针对pH变化的荧光光谱扫描图。
图3是实施例3制备的罗丹明pH荧光探针对pH变化的荧光光谱扫描图。
图4是实施例4制备的罗丹明pH荧光探针对pH变化的荧光光谱扫描图。
图5是实施例5制备的罗丹明pH荧光探针对pH变化的荧光光谱扫描图。
图1-图5是在日本岛津荧光光谱仪上得到的扫描图。
具体实施方式
实施例1制备含有谷氨酸结构的罗丹明pH荧光探针
本实施例的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
第一步:将0.002摩尔的罗丹明110与0.012摩尔的POCl3加入到20ml乙腈中,氮气保护下,加热到60度,保温反应3-5个小时,TLC监控至反应完全,原料消失。降至室温,缓慢加入0.002摩尔的谷氨酸二甲酯和0.006摩尔的三乙胺的乙腈(10ml)溶液,室温反应三个小时,TLC监控至酰氯完全消失。把反应液浓缩至干,加入30ml乙酸乙酯和20ml水,萃取分层,水层用20ml水萃取两次,合并有机层,无水硫酸钠干燥,浓缩至液体体积剩余一半,停止浓缩,降温,析晶,得白色固体。LCMS结果:489.2.4(M+2),488.2(M+1)。
第二步:将上一步所得白色固体溶于20ml水中,加入10ml30%氢氧化钠溶液,加热至50-60度,反应过夜,TLC监控至原料完全消失。降至室温,加入25ml10%稀HCl溶液,搅拌0.5h.加入80ml二氯甲烷溶液萃取,有机层用无水硫酸钠干燥,浓缩至干,得本实施例的目标产物。LCMS结果:460.9(M+2),459.9(M+1)。
实施例2制备含有谷氨酸结构的罗丹明pH荧光探针
本实施例的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
第一步:将0.002摩尔的罗丹明6G与0.01摩尔的POCl3加入到20ml乙腈中,氮气保护下,加热到60度,保温反应3-5个小时,TLC监控至反应完全,原料消失。降至室温,缓慢加入0.002摩尔的谷氨酸二甲酯和0.008摩尔的三乙胺的乙腈(10ml)溶液,室温反应三个小时,TLC监控至酰氯完全消失。把反应液浓缩至干,加入30ml乙酸乙酯和20ml水,萃取分层,水层用20ml水萃取两次,合并有机层,无水硫酸钠干燥,浓缩至液体体积剩余一半,停止浓缩,降温,析晶,得白色固体。LCMS结果:601.4(M+2),600.4(M+1)。
第二步:将上一步所得白色固体溶于20ml水中,加入10ml30%氢氧化钠溶液,加热至50-60度,反应过夜,TLC监控至原料完全消失。降至室温,加入25ml10%稀HCl溶液,搅拌0.5h.加入80ml二氯甲烷溶液萃取,有机层用无水硫酸钠干燥,浓缩至干,得本实施例的目标产物。LCMS结果:573.15(M+2),572.1(M+1)。
实施例3制备含有谷氨酸结构的罗丹明pH荧光探针
本实施例的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
第一步:将0.002摩尔的四甲基罗丹明TMR与0.008摩尔的POCl3加入到20ml乙腈中,氮气保护下,加热到60度,保温反应3-5个小时,TLC监控至反应完全,原料消失。降至室温,缓慢加入0.002摩尔的谷氨酸二甲酯和0.01摩尔的三乙胺的乙腈(10ml)溶液,室温反应三个小时,TLC监控至酰氯完全消失。把反应液浓缩至干,加入30ml乙酸乙酯和20ml水,萃取分层,水层用20ml水萃取两次,合并有机层,无水硫酸钠干燥,浓缩至液体体积剩余一半,停止浓缩,降温,析晶,得白色固体。LCMS结果:552.8(M+2),551.8(M+1)。
第二步:将上一步所得白色固体溶于20ml水中,加入10ml30%氢氧化钠溶液,加热至50-60度,反应过夜,TLC监控至原料完全消失。降至室温,加入25ml10%稀HCl溶液,搅拌0.5h.加入80ml二氯甲烷溶液萃取,有机层用无水硫酸钠干燥,浓缩至干,得本实施例的目标产物。LCMS结果:524.6(M+2),523.6(M+1)。
实施例4制备含有谷氨酸结构的罗丹明pH荧光探针
本实施例的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
第一步:将0.002摩尔的罗丹明B与0.006摩尔的POCl3加入到20ml乙腈中,氮气保护下,加热到60度,保温反应3-5个小时,TLC监控至反应完全,原料消失。降至室温,缓慢加入0.002摩尔的谷氨酸二甲酯和0.012摩尔的三乙胺的乙腈(10ml)溶液,室温反应三个小时,TLC监控至酰氯完全消失。把反应液浓缩至干,加入30ml乙酸乙酯和20ml水,萃取分层,水层用20ml水萃取两次,合并有机层,无水硫酸钠干燥,浓缩至液体体积剩余一半,停止浓缩,降温,析晶,得白色固体。LCMS结果:601.4(M+2),600.4(M+1)。
第二步:将上一步所得白色固体溶于20ml水中,加入10ml30%氢氧化钠溶液,加热至50-60度,反应过夜,TLC监控至原料完全消失。降至室温,加入25ml10%稀HCl溶液,搅拌0.5h.加入80ml二氯甲烷溶液萃取,有机层用无水硫酸钠干燥,浓缩至干,得本实施例的目标产物。LCMS结果:573.15(M+2),572.1(M+1)。
实施例5制备含有谷氨酸结构的罗丹明pH荧光探针
本实施例的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,包括如下步骤:
第一步:将0.002摩尔的罗丹明101与0.009摩尔的POCl3加入到20ml乙腈中,氮气保护下,加热到60度,保温反应3-5个小时,TLC监控至反应完全,原料消失。降至室温,缓慢加入0.002摩尔的谷氨酸二甲酯和0.009摩尔的三乙胺的乙腈(10ml)溶液,室温反应三个小时,TLC监控至酰氯完全消失。把反应液浓缩至干,加入30ml乙酸乙酯和20ml水,萃取分层,水层用20ml水萃取两次,合并有机层,无水硫酸钠干燥,浓缩至液体体积剩余一半,停止浓缩,降温,析晶,得白色固体。LCMS结果:649.4(M+2),648.4(M+1)。
第二步:将上一步所得白色固体溶于20ml水中,加入10ml30%氢氧化钠溶液,加热至50-60度,反应过夜,TLC监控至原料完全消失。降至室温,加入25ml10%稀HCl溶液,搅拌0.5h.加入80ml二氯甲烷溶液萃取,有机层用无水硫酸钠干燥,浓缩至干,得本实施例的目标产物。LCMS结果:621.2.2(M+2),620.2(M+1)。
实施例6测定罗丹明pH荧光探针对pH变化的荧光的光谱响应
本实施例的试验采用实施例1-5制备的pH荧光指示剂进行。
荧光光谱的测定方法:配制浓度为5X10-5mol/L的含有30%乙醇的PH荧光指示剂水溶液,该指示剂溶液分别用氢氧化钠和盐酸调节pH,分别制备pH范围在3.2-8.5不同pH值的溶液,测试荧光光谱。
实施例1的罗丹明pH荧光探针对pH变化的荧光的光谱响应结果如图1所示:图1中每条线分别代表不同pH值的测试结果,最下端的线代表pH为8.5时的荧光光谱曲线,往上pH值依次降低,依次为pH为8.0、7.0、6.2、5.5、5.0、4.5、4.0、3.2时的荧光光谱曲线,最上端的曲线对应的pH值为3.2,结果表明,随着pH值的降低,荧光强度不断增强。荧光指示剂在pH处于3.2—8.5范围内较为敏感,其荧光强度增强约60倍。由图1可以看出,其最强发射波长在640-650um。
实施例2的罗丹明pH荧光探针对pH变化的荧光的光谱响应结果如图2所示:图2中每条线分别代表不同pH值的测试结果,最下端的线代表pH为8.5时的荧光光谱曲线,往上pH值依次降低,依次为pH为8.0、7.0、6.2、5.5、5.0、4.5、4.0、3.2时的荧光光谱曲线,最上端的曲线对应的pH值为3.2,结果表明,随着pH值的降低,荧光强度不断增强。荧光指示剂在pH处于3.2—8.5范围内较为敏感,其荧光强度增强约60倍。由图2可以看出,其最强发射波长在560um左右。
实施例3的罗丹明pH荧光探针对pH变化的荧光的光谱响应结果如图3所示:图3中每条线分别代表不同pH值的测试结果,最下端的线代表pH为8.5时的荧光光谱曲线,往上pH值依次降低,依次为pH为8.0、7.0、6.2、5.5、5.0、4.5、4.0、3.2时的荧光光谱曲线,最上端的曲线对应的pH值为3.2,结果表明,随着pH值的降低,荧光强度不断增强。荧光指示剂在pH处于3.2—8.5范围内较为敏感,其荧光强度增强约60倍。由图3可以看出,其最强发射波长在540-550um左右。
实施例4的罗丹明pH荧光探针对pH变化的荧光的光谱响应结果如图4所示:图4中每条线分别代表不同pH值的测试结果,最下端的线代表pH为8.5时的荧光光谱曲线,往上pH值依次降低,依次为pH为8.0、7.0、6.2、5.5、5.0、4.5、4.0、3.2时的荧光光谱曲线,最上端的曲线对应的pH值为3.2,结果表明,随着pH值的降低,荧光强度不断增强。荧光指示剂在pH处于3.2—8.5范围内较为敏感,其荧光强度增强约60倍。由图4可以看出,其最强发射波长在560-570um左右。
实施例5的罗丹明pH荧光探针对pH变化的荧光的光谱响应结果如图5所示:图5中每条线分别代表不同pH值的测试结果,最下端的线代表pH为8.5时的荧光光谱曲线,往上pH值依次降低,依次为pH为8.0、7.0、6.2、5.5、5.0、4.5、4.0、3.2时的荧光光谱曲线,最上端的曲线对应的pH值为3.2,结果表明,随着pH值的降低,荧光强度不断增强。荧光指示剂在pH处于3.2—8.5范围内较为敏感,其荧光强度增强约60倍。由图5可以看出,其最强发射波长在560-570um左右。
综上所述,上述实施例1-5的罗丹明PH荧光探针根据在不同的PH值下荧光强度都能够增强到60-70倍,不同发射波长在生物培养领域中将非常广泛。其中图1所在荧光发射范围和荧光强度更佳。
Claims (4)
1.一种含有谷氨酸结构的罗丹明pH荧光探针,即Rh-pH荧光探针,其特征在于,所述的Rh-pH荧光探针的结构通式如(I)所示:
其中,
R1=R2=R3=R4=H;
或R1=R4=H,R2=—CH2CH3,R3=—CH3;
或R1=R2=—CH3,R3=R4=H;
或R1=R2=—CH2CH3,R3=R4=H;
或R1=R2=R3=R4=—(CH2)3—。
2.如权利要求1所述的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,其特征在于,包括如下步骤:
1)将罗丹明类化合物与三氯氧磷加入到乙腈中,保温反应3-5小时,冷却至室温后,加入氨基酸类化合物的乙腈溶液和三乙胺的乙腈溶液,室温下反应3-5小时,反应液用乙酸乙酯和水萃取,取有机层,干燥,浓缩,降温,析晶,得到白色固体;
2)将该白色固体溶于水中,加入氢氧化钠溶液,加热至50-60℃,反应过夜,降至室温,加入稀盐酸溶液,搅拌,再加入二氯甲烷溶液萃取,取有机层,干燥,浓缩,得到目标产物Rh-pH荧光探针,
所述罗丹明类化合物为罗丹明110、罗丹明6G、四甲基罗丹明TMR、罗丹明B或罗丹明101,所述氨基酸类化合物为谷氨酸二甲酯。
3.根据权利要求2所述的含有谷氨酸结构的罗丹明pH荧光探针的制备方法,其特征在于,所述的罗丹明类化合物、三氯氧磷、氨基酸类化合物与三乙胺的摩尔比为1:(3-6):1:(3-6)。
4.如权利要求1所述的含有谷氨酸结构的罗丹明pH荧光探针作为荧光指示剂在监测微生物培养过程中pH值变化的应用。
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