CN105567204B - A method of dolostone reservoirs Central Plains oil recovery is improved using microbial flora - Google Patents
A method of dolostone reservoirs Central Plains oil recovery is improved using microbial flora Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
Abstract
The present invention relates to a kind of methods for improving dolostone reservoirs Central Plains oil recovery using microbial flora, and wherein microbial flora includes pseudomonas aeruginosa, Bacillus cercus, Bacillus subtillis, microbacterium, Escherichia coli, pseudomonas putida and bacillus licheniformis；After the microbial flora compounds in proportion, by expanding culture and fermented and cultured, the fermentation liquid of microbial flora is made；It mix with injection water, and after nutrient solution is added, injects dolostone reservoirs rock core, microbial flora to be implanted and dolostone reservoirs rock core carry out oil recovering after acting on.Microbial flora of the invention is capable of increasing pore diameter, length and the porosity of rock core, reduces viscosity of crude and oil water interfacial tension, and then improve the oil recovery factor of dolostone reservoirs；And has the advantages that zymotechnique is simple, low in cost, easy to operate, input-output ratio is high, is suitable for scale industrial production, have a good application prospect.
Description
Technical field
The invention belongs to the interleaving techniques fields of geological prospecting and microbe oil production, and in particular to a kind of to utilize microbial bacteria
The method that group improves dolostone reservoirs Central Plains oil recovery.
Background technique
Petroleum is a kind of important energy minerals, is played an important role in the development of the national economy, and industry is known as
Blood.Petroleum is defined as in petroleum industry a kind of being mixed based on hydrocarbon, with special odor, coloured
Flammable oily liquid.Petroleum primarily forms and is stored in underground, wherein the rock stratum that can generate petroleum is referred to as source bed,
Mainly it is made of hydrocarbon source rock；Reservoir is just named in the rock stratum that petroleum can be stored, and abbreviation reservoir, it must have reservoir space (hole
Gap) and reservoir space have certain connectivity (permeability).In the world it has been found that most of oil and gas reserves carry out auto-deposition
Rock stratum, wherein mostly important with sandstone and carbonate reservoir.According to statistics, in the world the oil-gas accumulation of more than half in carbonic acid
In rock salt stratum, wherein being again most, to account for about the 80% of carbonate formation oil and gas production with dolomite subterranean formation production.Therefore, needle
The weight that geological work has become world's oil-gas exploration is carried out to the stratum based on carbonate formation, especially dolomite
Point.China's dolomite reservoir series of strata distribution is extensive, accounts for nearly the 40% of the continental deposit rock gross area.There are more than 20 basins in the whole nation
Ground distribution dolomite stratum, dolomite reservoir petroleum resources amount are more than 30,000,000,000 tons of oil equivalents.Dolomite exploration of oil and gas field
Exploitation has become China instantly and an emphasis of the following petroleum industry.
Oil recovery is the process for coming out petroleum from underground mining.Oil recovery step is divided into primary, secondary and tertiary oil recovery at present.
Primary oil recovery is using subsurface rock pressure itself is fuel-displaced, it is fuel-displaced that secondary oil recovery is that water filling generates pressure, but worldwide,
The 30-40% that original oil in place can only be produced by both traditional handicrafts, the Residual oil being retained in bottom still account for 60-
70%, remain in the petroleum in oil reservoir and be difficult to exploit due to being adsorbed between blowhole, how to improve oil recovery factor, become for
The most important thing of oil field development.All there is an urgent need to a kind of tertiary oil recovery technologies that can improve oil yield for each state, and conventional adopts three times
Oily method has: heat is driven, and steam flooding, the chemical displacement of reservoir oil (including surfactant flooding and polymer displacement of reservoir oil) and microorganism are adopted
Oil.
Microbial Enhanced Oil Recovery (Microbial Enhanced Oil Recovery, MEOR) is to utilize
Breeding of the microorganism in oil reservoir and metabolite improve a kind of biotechnology of crude output, which has the scope of application
Extensively, simple process, flexible operation mode, when not injuring stratum, free from environmental pollution, ecological safety, quick, effect of increasing production persistently
Between it is long the features such as, therefore have received universal attention.
Patent 201110096394 is related to a kind of oil extraction microbial using compounding and improves viscous crude and special thick oil recovery ratio
New method.The patent is mainly for ground viscosity >=5000mPas viscous crude and ground viscosity >=50000mPas or more
Special thick oil.The compounding functional microorganism is in laboratory screening to the special function that can significantly reduce viscous crude and special thick oil viscosity
It can hybrid bacterial strain.Nutrient solution by microorganism is compounded and containing abundant carbon source is injected in oil reservoir by special process, passes through micro- life
The active material generated in object breeding and metabolic process, can make viscosity of crude reduce 50-95%, improve the mobility of crude oil to reach
To the purpose for improving viscous crude and special thick oil block recovery ratio.
Patent 201210459111 is related to a kind of microbe oil production method for being continuously injected into low concentration nutrient solution.The patent is first
First pass through makes the micro organism quantity in stratum reach 10 to target reservoir injection high concentration bacterium solution and/or nutrient solution5~107A/
Then mL maintains microorganism in stratum by being continuously injected into 0.2% low concentration nutrient solution to set up oil pool microorganisms field
Growth and breeding, to achieve the purpose that microorganism and its metabolite to crude oil continuous action, extend microorganism in oil reservoir
It recovers the oil validity period, improves input-output ratio.Field application shows can have using the low concentration nutrient solution for being continuously injected into 0.2%
Effect promotes the growth and breeding of microorganism, avoids the micro organism quantity due to caused by the injection of slug formula and fluctuates larger, this method
Recovery ratio amplitude is improved in the case where not increasing cost reaches 7.0%.
Patent 201210040107 is related to a kind of acted synergistically using the composite bacterial solution containing phosphorus decomposing and nitrogen-fixing bacteria to improve original
The method of oil recovery compounds with petroleum hydrocarbon degradation bacterium pseudomonad, nitrogen-fixing bacteria with phosphate solubilization, after compounding
Complex micro organism fungicide is injected inside oil reservoir by water injection well or producing well through injection water dilution, improves single well productivity；It is above-mentioned multiple
Close microbial bacterial agent are as follows: compound micro- made of the ratio of pseudomonad, nitrogen-fixing bacteria and petroleum hydrocarbon degradation bacterium with 1: 1.2: 1.6~8
Bacteria agent；Above-mentioned petroleum hydrocarbon degradation bacterium includes bacillus, acinetobacter calcoaceticus and Xanthomonas campestris, and above-mentioned 3 kinds of bacterium are individually cultivated
At 108-1010The single bacterium liquid of cfu/ml, is then mixed to get petroleum hydrocarbon degradation bacterium in equal volume.The method of the present invention construction is simple, when
Between it is short, at low cost, amount of increase in production is big, tar productivity is high.
Patent 201310096006 is related to a kind of method for improving oil recovery factor, before oil extraction, for oil field 0
The shallow-layer rock core of~800m depth injects the aerobic microbiological with silicate mineral capacity of decomposition；For oil field 800m or more
The deep layer rock core of depth injects the anaerobe with iron reducing power；Microorganism and Clay Mineral to be implanted makees
Oil extraction is carried out again later with a period of time.The present invention is based on microorganisms to promote oil reservoir mineral montmorillonite object inversion of phases
Research, by improve reservoir water-sensitive expansion characteristics, for Oil in Super-low Permeability Oil Layer Reservoir contract it is swollen with improve oil recovery factor open it is new
Approach.
Patent 201410584084 is related to a kind of method that microbial oil displacement improves oil recovery factor, belongs to microbe oil production
Technical field, method includes the following steps: test block field sampling and endogenous microbes detection, the endogenous microbes of detection
Including aerobic, amphimicrobian and anaerobe；The aerobic microbiological displacement of reservoir oil stage in oil reservoir, to oil reservoir inject aerobic microbiological or
Its activator and air detect the aerobic microbiological in oil reservoir production fluid；The amphimicrobe displacement of reservoir oil stage in oil reservoir, to oil
Hiding injection aerobic-anaerobic microbe or its activator, detect aerobic-anaerobic microbe in oil reservoir production fluid；Anaerobe displacement of reservoir oil rank in oil reservoir
Section injects anaerobe or its activator to oil reservoir.The activator source that the present invention uses is wide, cheap and do not injure ground
Layer, the present invention have simple process, specific aim and strong operability, and field test results are good, and it is big that field test improves recovery ratio
In 10.0%, therefore, can be widely used in microbial carbonates field test.
Although more existing patented technologies report Microbial Enhanced Oil Recovery, specifically for low hole
The dolostone reservoirs of the features such as hyposmosis, cementing densification, but rarely have document report.
Summary of the invention
The present inventor combines modern biotechnology to provide using microbial flora and dolostone reservoirs reciprocation
A kind of method improving dolostone reservoirs Central Plains oil recovery using microbial flora, to be applied to domestic and international geological condition phase
The exploitation in all kinds of oil fields of symbol.
The object of the present invention is to provide it is a kind of using microbial flora improve dolostone reservoirs Central Plains oil recovery method,
Being related to microorganism includes pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacillus cercus (Bacillus
Cereus), Bacillus subtillis (Bacillus subtilis), microbacterium (Microbacterium sp.), Escherichia coli
(Escherichia coli), pseudomonas putida (Pseudomonas putida) and bacillus licheniformis (Bacillus
licheniformis)。
Further, pseudomonas aeruginosa in the microbial flora: Bacillus cercus: Bacillus subtillis: microbot
Bacterium: Escherichia coli: pseudomonas putida: the weight proportion of bacillus licheniformis is (5~15): (2~10): (1~10): (0.5
~8): (0.5~5): (0.3~1): (0.3~1)；Further, pseudomonas aeruginosa in the microbial flora: wax-like
Bacillus: Bacillus subtillis: microbacterium: Escherichia coli: pseudomonas putida: the weight proportion of bacillus licheniformis is (8
~12): (4~7): (3~8): (2~5): (1~3): (0.5~0.8): (0.5~0.7)；Still further, the microorganism
Pseudomonas aeruginosa in flora: Bacillus cercus: Bacillus subtillis: microbacterium: Escherichia coli: pseudomonas putida: ground
The weight proportion of clothing bacillus is 10: 6: 5: 3: 2: 0.7: 0.6.
A kind of method improving dolostone reservoirs Central Plains oil recovery using microbial flora provided by the present invention, it is described
After microbial flora compounds by weight ratio, first it is inoculated in culture tank with 2~4% w/v, expansion culture 12~
For 24 hours, 150~200rpm of culture tank revolving speed, controls 0.1~0.5mmol/ of oxygen content by 30~35 DEG C of cultivation temperature in incubation
L；It is inoculated in fermentor, 24~48h of fermented and cultured, fermentor 30~80rpm of revolving speed, is sent out with 5~8% w/v afterwards
30~35 DEG C of ferment temperature, 0.1~0.5mmol/L of oxygen content is controlled in fermentation process, and the fermentation liquid of microbial flora is made.Into one
Step ground after the microbial flora compounds by weight ratio, is first inoculated in culture tank with 3% w/v, expands training
16h, culture tank revolving speed 180rpm are supported, 32 DEG C of cultivation temperature, oxygen content 0.2mmol/L is controlled in incubation；Afterwards with 6% weight
Amount volume ratio is inoculated in fermentor, and fermented and cultured 36h, fermentor revolving speed 50rpm, are controlled in fermentation process by 32 DEG C of fermentation temperature
The fermentation liquid of microbial flora is made in oxygen content 0.2mmol/L processed.
Further, it is described expand culture culture medium be 5~10g/L of sucrose, 1~3g/L of beef extract, peptone 1~
3g/L、(NH4)2SO40.5~0.8g/L, MgSO40.3~0.5g/L, K2HPO40.2~0.4g/L, KH2PO40.1~0.3g/L,
CaCl20.1~0.2g/L, surplus are water；Further, the expansion culture medium of the microbial flora is sucrose 8g/L, ox
Meat extract 2g/L, peptone 2g/L, (NH4)2SO40.6g/L、MgSO40.4g/L、K2HPO40.3g/L、KH2PO40.2g/L、
CaCl20.1g/L, surplus are water.
Further, the fermentation medium of the microbial flora is 10~20g/L of sucrose, 5~10g/L of glucose, ox
3~5g/L of meat extract, 3~5g/L of peptone, 6~8g/L of corn pulp, MgSO40.5~0.8g/L, K2HPO40.2~0.5g/L,
KH2PO40.2~0.5g/L, NH40.1~0.3g/L of Cl, CaCl20.1~0.2g/L, surplus are water；Further, described
The fermentation medium of microbial flora is sucrose 15g/L, glucose 8g/L, beef extract 4g/L, peptone 4g/L, corn pulp 7g/
L、MgSO40.7g/L、K2HPO40.4g/L、KH2PO40.3g/L、NH4Cl0.2g/L、CaCl20.2g/L, surplus are water.
A kind of method improving dolostone reservoirs Central Plains oil recovery using microbial flora provided by the present invention, it is special
Sign is that the fermentation liquid of microbial flora obtained is pressed 1: the volume ratio of (800~1200) is mixed with injection water, and is added 1
After the nutrient solution of~5% volume ratio, dolostone reservoirs rock core is injected, microbial flora to be implanted and dolostone reservoirs rock core are made
Oil recovering is carried out with after 10~30 days.Further, the fermentation liquid of microbial flora obtained is pressed to 1: 1000 volume ratio
It is mixed with injection water, and after the nutrient solution of 2% volume ratio of addition, injects dolostone reservoirs rock core, microbial flora to be implanted
Oil recovering is carried out after acting on 15 days with dolostone reservoirs rock core.
Further, the composition of the nutrient solution includes: 15~20g/L of glucose, 15~20g/L of corn pulp, NH4Cl
0.5~0.8g/L, KH2PO40.5~0.8g/L, surplus are water.Further, the composition of the nutrient solution includes: glucose
18g/L, corn pulp 18g/L, NH4Cl 0.6g/L、KH2PO40.6g/L, surplus are water.
The present inventor learnt by property experiment to each microbial bacteria and analysis, pseudomonas aeruginosa in the present invention
(Pseudomonas aeruginosa) and Bacillus cercus (Bacillus cereus) have point of carbonate, silicate
Solution ability can react and increase the permeability and porosity of matrix with the mineral in dolomite；Bacillus subtillis
(Bacillus subtilis) and microbacterium (Microbacterium sp.) have the ability of degradation long chain hydrocarbons, can reduce
Viscosity of crude in dolostone reservoirs；Escherichia coli (Escherichia coli) have the ability of production long chain fatty acids, energy
Enough reduce crude oil and dolomite, the interface of crude oil and injection water engaging surface and surface tension；Pseudomonas putida
(Pseudomonas putida) and bacillus licheniformis (Bacillus licheniformis) are equivalent to a kind of " auxiliary agent ", energy
Enough mentioned microorganism bacterium is assisted to realize its purpose and effect.Above-mentioned seven kinds of microbial bacterias complement each other, collective effect.
Acquired having the technical effect that of the invention uses microbial flora fermentation liquor treatment dolostone reservoirs of the invention
Rock core is capable of increasing pore diameter, length and the porosity size of rock core；Viscosity of crude and oil water interfacial tension can also be reduced；
And then improve the oil recovery factor of dolostone reservoirs.And the present invention have zymotechnique it is simple, it is low in cost, easy to operate,
The advantages that input-output ratio is high is suitable for scale industrial production, has a good application prospect.
Specific embodiment
Technology contents of the invention are described further below with reference to embodiment；Following embodiments be it is illustrative, no
It is restrictive, cannot be limited the scope of protection of the present invention with following embodiments.
Embodiment 1
By weight ratio 5: 2: 1: 0.5: 0.5: 0.3: 0.3 weighs pseudomonas aeruginosa, Bacillus cercus, Ko subtilis
Bacillus, microbacterium, Escherichia coli, pseudomonas putida, bacillus licheniformis mix, and are first inoculated in 2% w/v
In culture tank, expands culture 12h, culture tank revolving speed 150rpm and 32 DEG C of cultivation temperature, oxygen content is controlled in incubation
0.1mmol/L；It is inoculated in fermentor with 5% w/v afterwards, for 24 hours, fermentor revolving speed 30rpm's fermented and cultured ferments
32 DEG C of temperature, oxygen content 0.1mmol/L is controlled in fermentation process, and the fermentation liquid of microbial flora is made.Expand the culture of culture
Base is sucrose 5g/L, beef extract 1g/L, peptone 1g/L, (NH4)2SO40.5g/L、MgSO40.3g/L、K2HPO40.2g/L、
KH2PO40.1g/L、CaCl20.1g/L, surplus are water；Fermentation medium be sucrose 10g/L, glucose 5g/L, beef extract 3g/L,
Peptone 3g/L, corn pulp 6g/L, MgSO40.5g/L、K2HPO40.2g/L、KH2PO40.2g/L、NH4Cl 0.1g/L、
CaCl20.1g/L, surplus are water.
Embodiment 2
By weight ratio 15: 10: 10: 8: 5: 1: 1 weigh pseudomonas aeruginosa, Bacillus cercus, Bacillus subtillis,
Microbacterium, Escherichia coli, pseudomonas putida, bacillus licheniformis mix, and are first inoculated in culture with 4% w/v
In tank, expand culture for 24 hours, culture tank revolving speed 200rpm, controls oxygen content 0.5mmol/L by 32 DEG C of cultivation temperature in incubation；
It is inoculated in fermentor, fermented and cultured 48h, fermentor revolving speed 80rpm, 32 DEG C of fermentation temperature, is sent out with 8% w/v afterwards
Oxygen content 0.5mmol/L is controlled during ferment, and the fermentation liquid of microbial flora is made.The culture medium for expanding culture is sucrose 10g/
L, beef extract 3g/L, peptone 3g/L, (NH4)2SO40.8g/L、MgSO40.5g/L、K2HPO40.4g/L、KH2PO40.3g/L、
CaCl20.2g/L, surplus are water；Fermentation medium be sucrose 20g/L, glucose 10g/L, beef extract 5g/L, peptone 5g/L,
Corn pulp 8g/L, MgSO40.8g/L、K2HPO40.5g/L、KH2PO40.5g/L、NH4Cl 0.3g/L、CaCl20.2g/L, surplus
For water.
Embodiment 3
By weight ratio 8: 4: 3: 2: 1: 0.5: 0.5 weighs pseudomonas aeruginosa, Bacillus cercus, Ko subtilis bar
Bacterium, microbacterium, Escherichia coli, pseudomonas putida, bacillus licheniformis mix, and are first inoculated in training with 3% w/v
It supports in tank, expands culture 16h, culture tank revolving speed 180rpm, 32 DEG C of cultivation temperature, oxygen content 0.2mmol/ is controlled in incubation
It is inoculated in fermentor after L with 6% w/v, fermented and cultured 36h, fermentor revolving speed 50rpm, 32 DEG C of fermentation temperature,
Oxygen content 0.2mmol/L is controlled in fermentation process, and the fermentation liquid of microbial flora is made.Expanding culture medium is sucrose 8g/L, ox
Meat extract 2g/L, peptone 2g/L, (NH4)2SO40.6g/L、MgSO40.4g/L、K2HPO40.3g/L、KH2PO40.2g/L、
CaCl20.1g/L, surplus are water；Fermentation medium be sucrose 15g/L, glucose 8g/L, beef extract 4g/L, peptone 4g/L,
Corn pulp 7g/L, MgSO40.7g/L、K2HPO40.4g/L、KH2PO40.3g/L、NH4Cl 0.2g/L、CaCl20.2g/L, surplus
For water.
Embodiment 4
By weight ratio 12: 7: 8: 5: 3: 0.8: 0.7 weighs pseudomonas aeruginosa, Bacillus cercus, Ko subtilis bar
Bacterium, microbacterium, Escherichia coli, pseudomonas putida, bacillus licheniformis mix, and are first inoculated in training with 3% w/v
It supports in tank, expands culture 16h, culture tank revolving speed 180rpm, 32 DEG C of cultivation temperature, oxygen content 0.2mmol/ is controlled in incubation
L；It is inoculated in fermentor with 6% w/v afterwards, fermented and cultured 36h, fermentor revolving speed 50rpm, 32 DEG C of fermentation temperature,
Oxygen content 0.2mmol/L is controlled in fermentation process, and the fermentation liquid of microbial flora is made.Expanding culture medium is sucrose 8g/L, ox
Meat extract 2g/L, peptone 2g/L, (NH4)2SO40.6g/L、MgSO40.4g/L、K2HPO40.3g/L、KH2PO40.2g/L、
CaCl20.1g/L, surplus are water；Fermentation medium be sucrose 15g/L, glucose 8g/L, beef extract 4g/L, peptone 4g/L,
Corn pulp 7g/L, MgSO40.7g/L、K2HPO40.4g/L、KH2PO40.3g/L、NH4Cl 0.2g/L、CaCl20.2g/L, surplus
For water.
Embodiment 5
By weight ratio 10: 6: 5: 3: 2: 0.7: 0.6 weighs pseudomonas aeruginosa, Bacillus cercus, Ko subtilis bar
Bacterium, microbacterium, Escherichia coli, pseudomonas putida, bacillus licheniformis mix, and are first inoculated in training with 3% w/v
It supports in tank, expands culture 16h, culture tank revolving speed 180rpm, 32 DEG C of cultivation temperature, oxygen content 0.2mmol/ is controlled in incubation
L；It is inoculated in fermentor with 6% w/v afterwards, fermented and cultured 36h, fermentor revolving speed 50rpm, 32 DEG C of fermentation temperature,
Oxygen content 0.2mmol/L is controlled in fermentation process, and the fermentation liquid of microbial flora is made.Expanding culture medium is sucrose 8g/L, ox
Meat extract 2g/L, peptone 2g/L, (NH4)2SO40.6g/L、MgSO40.4g/L、K2HPO40.3g/L、KH2PO40.2g/L、
CaCl20.1g/L, surplus are water；Fermentation medium be sucrose 15g/L, glucose 8g/L, beef extract 4g/L, peptone 4g/L,
Corn pulp 7g/L, MgSO40.7g/L、K2HPO40.4g/L、KH2PO40.3g/L、NH4Cl 0.2g/L、CaCl20.2g/L, surplus
For water.
Embodiment 6
The fermentation liquid of microbial flora made from embodiment 3 is mixed by 1: 800 volume ratio with injection water, and is added
After the nutrient solution of 1% volume ratio, dolostone reservoirs rock core is injected, microbial flora to be implanted and dolostone reservoirs rock core act on
Oil recovering is carried out after 10 days；The composition of the nutrient solution includes: glucose 15g/L, corn pulp 15g/L, NH4Cl 0.5g/L、
KH2PO40.5g/L, surplus are water.
Embodiment 7
The fermentation liquid of microbial flora made from embodiment 4 is mixed by 1: 1200 volume ratio with injection water, and is added
After the nutrient solution of 5% volume ratio, dolostone reservoirs rock core is injected, microbial flora to be implanted and dolostone reservoirs rock core act on
Oil recovering is carried out after 30 days；The composition of the nutrient solution includes: glucose 20g/L, corn pulp 20g/L, NH4Cl 0.8g/L、
KH2PO40.8g/L, surplus are water.
Embodiment 8
The fermentation liquid of microbial flora made from embodiment 5 is mixed by 1: 1000 volume ratio with injection water, and is added
After the nutrient solution of 2% volume ratio, injection is acted on from cloud rock reservoir core, microbial flora to be implanted and dolostone reservoirs rock core
Oil recovering is carried out after 15 days；The composition of the nutrient solution includes: glucose 18g/L, corn pulp 18g/L, NH4Cl 0.6g/、
KH2PO40.6g/L, surplus are water.
Test example 1
By the fermentation liquid of the embodiment 3-5 microbial flora prepared add water by 1: 1000 dilution proportion after, add 2%
Nutrient solution (the glucose 18g/L, corn pulp 18g/L, NH of volume ratio4Cl 0.6g/L、KH2PO40.6g/L, surplus are water) after,
Respectively be made soak 1. -3..
Take dolostone reservoirs rock core be soaked in the soak 1. -3. in, it is closed save 15 days after, measure dolostone reservoirs rock
The situation of change of the pore diameter of the heart, length and porosity, the results are shown in Table 1 (n=5).
Table 1
It can be obtained according to 1 result of table, using microbial flora fermentation liquor treatment dolostone reservoirs rock core of the invention, Neng Gouzeng
Pore diameter, length and the porosity size of big rock core.
Test example 2
By soak prepared by test example 1 1. -3. mixed by 1% volume ratio and the crude oil containing dolostone reservoirs rock core
It closes, measures the situation of change of viscosity of crude and oil water interfacial tension at room temperature, the results are shown in Table 2 (n=5).
Table 2
The crude oil for containing dolostone reservoirs rock core using microbial flora fermentation liquor treatment of the invention, can reduce crude oil
Viscosity and oil water interfacial tension.
Claims (10)
1. a kind of method for improving dolostone reservoirs Central Plains oil recovery using microbial flora, which is characterized in that micro- life
The composition of object flora are as follows: pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacillus cercus (Bacillus
Cereus), Bacillus subtillis (Bacillus subtilis), microbacterium (Microbacterium sp.), Escherichia coli
(Escherichia coli), pseudomonas putida (Pseudomonas putida) and bacillus licheniformis (Bacillus
licheniformis)。
2. the method according to claim 1 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is, pseudomonas aeruginosa in the microbial flora: Bacillus cercus: Bacillus subtillis: microbacterium: Escherichia coli
: pseudomonas putida: the weight proportion of bacillus licheniformis is (5~15): (2~10): (1~10): (0.5~8): (0.5~
5): (0.3~1): (0.3~1).
3. the method according to claim 1 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is, pseudomonas aeruginosa in the microbial flora: Bacillus cercus: Bacillus subtillis: microbacterium: Escherichia coli
: pseudomonas putida: the weight proportion of bacillus licheniformis is (8~12): (4~7): (3~8): (2~5): (1~3):
(0.5~0.8): (0.5~0.7).
4. the method according to claim 1 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is, pseudomonas aeruginosa in the microbial flora: Bacillus cercus: Bacillus subtillis: microbacterium: Escherichia coli
: pseudomonas putida: the weight proportion of bacillus licheniformis is 10: 6: 5: 3: 2: 0.7: 0.6.
5. improving dolostone reservoirs Central Plains oil recovery using microbial flora according to claim 2-4 any one
Method, which is characterized in that after the microbial flora compounds by weight ratio, first with 2~4% w/v inoculation son training
It supports in tank, expansion culture 12~for 24 hours, culture tank 150~200rpm of revolving speed, is controlled in incubation by 30~35 DEG C of cultivation temperature
0.1~0.5mmol/L of oxygen content；It is inoculated in fermentor, fermented and cultured 2448h, is fermented with 5~8% w/v afterwards
Tank 30~80rpm of revolving speed, controls 0.1~0.5mmol/L of oxygen content, micro- life is made by 30~35 DEG C of fermentation temperature in fermentation process
The fermentation liquid of object flora.
6. the method according to claim 5 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is that the culture medium for expanding culture is 5~10g/L of sucrose, 1~3g/L of beef extract, 1~3g/L of peptone, (NH4)2SO40.5~0.8g/L, MgSO40.3~0.5g/L, K2HPO40.2~0.4g/L, KH2PO40.1~0.3g/L, CaCl20.1~
0.2g/L, surplus are water.
7. the method according to claim 5 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is that the fermentation medium of the microbial flora is 10~20g/L of sucrose, 5~10g/L of glucose, 3~5g/ of beef extract
L, 3~5g/L of peptone, 6~8g/L of corn pulp, MgSO40.5~0.8g/L, K2HPO40.2~0.5g/L, KH2PO40.2~
0.5g/L、NH40.1~0.3g/L of Cl, CaCl20.1~0.2g/L, surplus are water.
8. improving dolostone reservoirs Central Plains oil recovery using microbial flora according to claim 6-7 any one
Method, the fermentation liquid of microbial flora obtained is pressed 1: the volume ratio of (800~1200) is mixed with injection water, and addition 1~
After the nutrient solution of 5% volume ratio, dolostone reservoirs rock core is injected, microbial flora to be implanted and dolostone reservoirs rock core act on
Oil recovering is carried out after 10~30 days.
9. the method according to claim 8 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is that the composition of the nutrient solution includes: 15~20g/L of glucose, 15~20g/L of corn pulp, NH40.5~0.8g/ of Cl
L、KH2PO40.5~0.8g/L, surplus are water.
10. the method according to claim 8 for improving dolostone reservoirs Central Plains oil recovery using microbial flora, special
Sign is that the composition of the nutrient solution includes: glucose 18g/L, corn pulp 18g/L, NH4Cl 0.6g/L、KH2PO40.6g/L,
Surplus is water.
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