CN105567204A - Method for improving crude oil recovery ratio in dolomite reservoir by using microbial flora - Google Patents
Method for improving crude oil recovery ratio in dolomite reservoir by using microbial flora Download PDFInfo
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Abstract
The invention relates to a method for improving a crude oil recovery ratio in a dolomite reservoir by using microbial flora, wherein the microbial flora include pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, microbacterium sp., escherichia coli, pseudomonas putida and bacillus licheniformis; the microbial flora are compounded according to the ratio, and through expansion culture and fermentation culture, a fermentation liquid of the microbial flora is prepared; the fermentation liquid is mixed with injected water, a nutrient solution is added into the mixed solution, the mixed solution is injected into a dolomite reservoir core, and after the injected microbial flora act on the dolomite reservoir core, crude oil recovery is performed. The microbial flora can increase the core pore diameter, length and porosity, and lowers the crude oil viscosity and oil-water interfacial tension, so as to improve the crude oil recovery ratio of the dolomite reservoir; moreover, the method has the advantages of simple fermentation process, low cost, simple operation, and high input/output ratio, is suitable for large-scale industrial production, and has good application prospects.
Description
Technical field
The invention belongs to the interleaving techniques field of geological prospecting and microbe oil production, be specifically related to a kind of method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio.
Background technology
Oil is a kind of important energy minerals, plays an important role in the development of the national economy, is described as the blood of industry.Oil being defined as by petroleum industry a kind of take hydrocarbon polymer as main that mix, that have special odor, coloured flammable oleaginous fluid.Oil is mainly formed and is stored in underground, and the rock stratum that wherein can produce oil is referred to as petroleum source bed, forms primarily of hydrocarbon source rock; Reservoir is just named in the rock stratum that can store oil, and be called for short reservoir, it must have reservoir space (porosity) and reservoir space has certain connectedness (perviousness).Found in the world oil and gas reserves great majority from sedimentary type formations, wherein with sandstone and carbonate reservoir the most important.According to statistics, oil-gas accumulation over half in the world, in carbonate formation, is wherein with dolomite subterranean formation production again, accounts for 80% of carbonate formation oil and gas production.Therefore, for carbonate formation, especially dolomite is that the emphasis that geological work has become world's oil-gas exploration is carried out on main stratum.China's dolomite reservoir series of strata distribution range is extensive, accounts for nearly 40% of the continental deposits rock total area.There are more than 20 Basin Developing distribution dolomite stratum in the whole nation, and dolomite reservoir hydrocarbon resources amount is more than 30,000,000,000 tons of oil equivalents.Dolomite oilfield prospecting developing has become China instantly and following petroleum industry emphasis.
Oil recovery is from underground mining process out by oil.Current oil recovery step is divided into once, secondary and tertiary oil recovery.Utilize that subsurface rock pressure itself is fuel-displaced is primary oil recovery, secondary oil recovery is that water filling generation pressure is fuel-displaced, but worldwide, the 30-40% of original oil in place can only be exploited out through these two kinds of traditional technologys, the Residual oil be retained in bottom still accounts for 60-70%, the oil remained in oil reservoir is difficult to exploitation owing to being adsorbed between blowhole, how to improve oil recovery factor, just becomes the most important thing of oil-field development.Each state is all in the urgent need to a kind of tertiary oil recovery technology that can improve oil yield, and conventional tertiary oil recovery method has: heat is driven, steam flooding, the chemical displacement of reservoir oil (comprising surfactant flooding and polymer flooding) and microbe oil production.
Microbial Enhanced Oil Recovery (MicrobialEnhancedOilRecovery, MEOR) be that the breeding utilizing microorganism in oil reservoir and meta-bolites are to improve a kind of biotechnology of crude production rate, this technology has that applied widely, technique is simple, flexible operation mode, do not injure the features such as stratum, free from environmental pollution, ecological safety, instant effect, effect of increasing production time length be long, be therefore subject to the most attention of people.
Patent 201110096394 relates to and a kind ofly utilizes composite oil extraction microbial to improve the novel method of viscous crude and special thick oil recovery ratio.The viscous crude of this patent mainly for ground viscosity >=5000mPas and the special thick oil of ground viscosity >=more than 50000mPas.This composite functional microorganism is to the specific function hybrid bacterial strain that significantly can reduce viscous crude and special thick oil viscosity in laboratory screening.Oil reservoir is injected by special process by composite microorganism and containing the nutritive medium enriching carbon source, by the active substance produced in microbial reproduction and metabolic process, viscosity of crude can be made to reduce 50-95%, improve the mobility of crude oil to reach the object improving viscous crude and special thick oil block recovery ratio.
Patent 201210459111 relates to a kind of microbe oil production method of continuous injection lower concentration nutritive medium.This patent is first by making the microbe population on stratum reach 10 to target reservoir injection high-concentration bacterial liquid and/or nutritive medium
5~ 10
7individual/mL, to set up oil pool microorganisms field, then the lower concentration nutritive medium by injecting 0.2% continuously maintains stratum microbial growth breeding, thus reach microorganism and meta-bolites thereof in oil reservoir to the object of crude oil continuous action, extend microbe oil production validity period, improve input-output ratio.Rig-site utilization shows, the lower concentration nutritive medium injecting 0.2% is continuously adopted can effectively to promote that microbial growth is bred, avoid the microbe population fluctuation caused due to the injection of slug formula comparatively large, the method improves recovery ratio amplitude and reaches 7.0% when not increasing cost.
Patent 201210040107 relates to a kind of method utilizing the composite bacterial solution synergy containing phosphorus decomposing and vinelandii to improve oil recovery factor, undertaken composite by having the pseudomonas of phosphate solubilization, vinelandii and petroleum hydrocarbon degradation bacterium, complex micro organism fungicide after composite is through injecting water dilution, inject oil reservoir by water injection well or recovery well inner, improve single well productivity; Above-mentioned complex micro organism fungicide is: pseudomonas, vinelandii and petroleum hydrocarbon degradation bacterium with 1: 1.2: 1.6 ~ 8 the complex micro organism fungicide made of ratio; Above-mentioned petroleum hydrocarbon degradation bacterium comprises genus bacillus, acinetobacter calcoaceticus and Xanthomonas campestris, and above-mentioned 3 kinds of bacterium single culture are become 10
8-10
10single bacterium liquid of cfu/ml, then equal-volume is mixed to get petroleum hydrocarbon degradation bacterium.The inventive method construction is simple, the time is short, cost is low, amount of increase in production is large, tar productivity is high.
Patent 201310096006 relates to a kind of method improving oil recovery factor, before Oil extraction, for the shallow-layer rock core of oil field 0 ~ 800m degree of depth, injects the aerobic microbiological with silicate minerals capacity of decomposition; For the deep layer rock core of the above degree of depth of oil field 800m, inject the anaerobion with Fe3+ reduction ability; Microorganism to be implanted and carry out Oil extraction again after Clay Mineral effect for some time.The present invention is based on the research that microorganism promotes oil reservoir mineral montmorillonite thing inversion of phases, by improving reservoir water-sensitive expansion characteristics, opening new approach for the contracting of Oil in Super-low Permeability Oil Layer Reservoir is swollen with raising oil recovery factor.
Patent 201410584084 relates to a kind of method that microbial oil displacement improves oil recovery factor, belong to Microbial Enhanced Oil Recovery field, the method comprises the following steps: test block on-site sampling and endogenous microbes detect, and the endogenous microbes of detection comprises aerobic, amphimicrobian and anaerobion; In the aerobic microbiological displacement of reservoir oil stage in oil reservoir, inject aerobic microbiological or its activator and air to oil reservoir, detect the aerobic microbiological in oil reservoir production fluid; In the amphimicrobe displacement of reservoir oil stage in oil reservoir, inject aerobic-anaerobic microbe or its activator to oil reservoir, detect aerobic-anaerobic microbe in oil reservoir production fluid; In the anaerobion displacement of reservoir oil stage in oil reservoir, inject anaerobion or its activator to oil reservoir.The activator source that the present invention adopts is wide, cheap and do not injure stratum, the present invention has that technique is simple, specific aim and workable, and field test results is good, and test in place improves recovery ratio and is greater than 10.0%, therefore, can be widely used in microbial carbonates test in place.
Although many sections of existing patented technologies report Microbial Enhanced Oil Recovery, special in the dolostone reservoirs with features such as low porosity and low permeability is saturating, cementing densifications, but rarely have bibliographical information.
Summary of the invention
The present inventor is in conjunction with modern biotechnology, utilize microorganism species and dolostone reservoirs interaction, provide a kind of method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio, to be applied to the exploitation in all kinds of oil fields that domestic and international geological condition conforms to.
The object of this invention is to provide a kind of method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio, relate to microorganism and comprise Pseudomonas aeruginosa (Pseudomonasaeruginosa), bacillus cereus (Bacilluscereus), Bacillus subtillis (Bacillussubtilis), microbacterium (Microbacteriumsp.), intestinal bacteria (Escherichiacoli), pseudomonas putida (Pseudomonasputida) and Bacillus licheniformis (Bacilluslicheniformis).
Further, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is (5 ~ 15): (2 ~ 10): (1 ~ 10): (0.5 ~ 8): (0.5 ~ 5): (0.3 ~ 1): (0.3 ~ 1); Further, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is (8 ~ 12): (4 ~ 7): (3 ~ 8): (2 ~ 5): (1 ~ 3): (0.5 ~ 0.8): (0.5 ~ 0.7); Again further, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is 10: 6: 5: 3: 2: 0.7: 0.6.
A kind of method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio provided by the present invention, after described microorganism species is composite by weight ratio, first be inoculated in culture tank with the weightmeasurement ratio of 2 ~ 4%, enlarged culturing 12 ~ 24h, culture tank rotating speed 150 ~ 200rpm, culture temperature 30 ~ 35 DEG C, controls oxygen level 0.1 ~ 0.5mmol/L in culturing process; After with 5 ~ 8% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 24 ~ 48h, fermentor tank rotating speed 30 ~ 80rpm, leavening temperature 30 ~ 35 DEG C, controls oxygen level 0.1 ~ 0.5mmol/L in fermenting process, the fermented liquid of obtained microorganism species.Further, after described microorganism species is composite by weight ratio, be first inoculated in culture tank with the weightmeasurement ratio of 3%, enlarged culturing 16h, culture tank rotating speed 180rpm, culture temperature 32 DEG C, controls oxygen level 0.2mmol/L in culturing process; After with 6% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 36h, fermentor tank rotating speed 50rpm, leavening temperature 32 DEG C, controls oxygen level 0.2mmol/L in fermenting process, the fermented liquid of obtained microorganism species.
Further, the substratum of described enlarged culturing is sucrose 5 ~ 10g/L, extractum carnis 1 ~ 3g/L, peptone 1 ~ 3g/L, (NH
4)
2sO
40.5 ~ 0.8g/L, MgSO
40.3 ~ 0.5g/L, K
2hPO
40.2 ~ 0.4g/L, KH
2pO
40.1 ~ 0.3g/L, CaCl
20.1 ~ 0.2g/L, surplus is water; Further, the enlarged culturing base of described microorganism species is sucrose 8g/L, extractum carnis 2g/L, peptone 2g/L, (NH
4)
2sO
40.6g/L, MgSO
40.4g/L, K
2hPO
40.3g/L, KH
2pO
40.2g/L, CaCl
20.1g/L, surplus is water.
Further, the fermention medium of described microorganism species is sucrose 10 ~ 20g/L, glucose 5 ~ 10g/L, extractum carnis 3 ~ 5g/L, peptone 3 ~ 5g/L, corn steep liquor 6 ~ 8g/L, MgSO
40.5 ~ 0.8g/L, K
2hPO
40.2 ~ 0.5g/L, KH
2pO
40.2 ~ 0.5g/L, NH
4cl0.1 ~ 0.3g/L, CaCl
20.1 ~ 0.2g/L, surplus is water; Further, the fermention medium of described microorganism species is sucrose 15g/L, glucose 8g/L, extractum carnis 4g/L, peptone 4g/L, corn steep liquor 7g/L, MgSO
40.7g/L, K
2hPO
40.4g/L, KH
2pO
40.3g/L, NH
4cl0.2g/L, CaCl
20.2g/L, surplus is water.
A kind of method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio provided by the present invention, it is characterized in that, by the fermented liquid of obtained microorganism species by 1: the volume ratio of (800 ~ 1200) mixes with injection water, and after adding the nutritive medium of 1 ~ 5% volume ratio, inject dolostone reservoirs rock core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 10 ~ 30 days.Further, the volume ratio of the fermented liquid of obtained microorganism species being pressed 1: 1000 mixes with injection water, and after adding the nutritive medium of 2% volume ratio, inject dolostone reservoirs rock core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 15 days.
Further, the composition of described nutritive medium comprises: glucose 15 ~ 20g/L, corn steep liquor 15 ~ 20g/L, NH
4cl0.5 ~ 0.8g/L, KH
2pO
40.5 ~ 0.8g/L, surplus is water.Further, the composition of described nutritive medium comprises: glucose 18g/L, corn steep liquor 18g/L, NH
4cl0.6g/L, KH
2pO
40.6g/L, surplus is water.
The present inventor is by learning the property experiment of each microbial bacteria and analysis, in the present invention, Pseudomonas aeruginosa (Pseudomonasaeruginosa) and bacillus cereus (Bacilluscereus) have the capacity of decomposition of carbonate, silicate, can react with the mineral in dolomite and increase rate of permeation and the porosity of matrix; Bacillus subtillis (Bacillussubtilis) and microbacterium (Microbacteriumsp.) have the ability of degraded long chain hydrocarbon, can reduce the viscosity of crude in dolostone reservoirs; Intestinal bacteria (Escherichiacoli) have the ability of producing longer chain fatty acid, can reduce the surface and interface tension force of crude oil and dolomite, crude oil and injection water engaging surface; Pseudomonas putida (Pseudomonasputida) and Bacillus licheniformis (Bacilluslicheniformis) are equivalent to one " auxiliary agent ", and mentioned microorganism bacterium can be assisted to realize its object and effect.Above-mentioned seven kinds of microbial bacterias complement each other, acting in conjunction.
Acquired technique effect of the present invention is: adopt microorganism species fermentation liquor treatment dolostone reservoirs rock core of the present invention, can increase the pore diameter of rock core, length and porosity size; Viscosity of crude and oil water interfacial tension can also be reduced; And then improve the oil recovery factor of dolostone reservoirs.And the present invention has, and zymotechnique is simple, with low cost, simple to operate, input-output ratio advantages of higher, is suitable for large-scale industrial and produces, have a good application prospect.
Embodiment
Below in conjunction with embodiment, technology contents of the present invention is described further; Following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Embodiment 1
Take Pseudomonas aeruginosa, bacillus cereus, Bacillus subtillis, microbacterium, intestinal bacteria, pseudomonas putida, Bacillus licheniformis at 5: 2: 1: 0.5: 0.5: 0.3: 0.3 by weight ratio, mixing, first be inoculated in culture tank with the weightmeasurement ratio of 2%, enlarged culturing 12h, culture tank rotating speed 150rpm, culture temperature 32 DEG C, controls oxygen level 0.1mmol/L in culturing process; After with 5% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 24h, fermentor tank rotating speed 30rpm, leavening temperature 32 DEG C, controls oxygen level 0.1mmol/L in fermenting process, the fermented liquid of obtained microorganism species.The substratum of enlarged culturing is sucrose 5g/L, extractum carnis 1g/L, peptone 1g/L, (NH
4)
2sO
40.5g/L, MgSO
40.3g/L, K
2hPO
40.2g/L, KH
2pO
40.1g/L, CaCl
20.1g/L, surplus is water; Fermention medium is sucrose 10g/L, glucose 5g/L, extractum carnis 3g/L, peptone 3g/L, corn steep liquor 6g/L, MgSO
40.5g/L, K
2hPO
40.2g/L, KH
2pO
40.2g/L, NH
4cl0.1g/L, CaCl
20.1g/L, surplus is water.
Embodiment 2
Take Pseudomonas aeruginosa, bacillus cereus, Bacillus subtillis, microbacterium, intestinal bacteria, pseudomonas putida, Bacillus licheniformis at 15: 10: 10: 8: 5: 1: 1 by weight ratio, mixing, first be inoculated in culture tank with the weightmeasurement ratio of 4%, enlarged culturing 24h, culture tank rotating speed 200rpm, culture temperature 32 DEG C, controls oxygen level 0.5mmol/L in culturing process; After with 8% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 48h, fermentor tank rotating speed 80rpm, leavening temperature 32 DEG C, controls oxygen level 0.5mmol/L in fermenting process, the fermented liquid of obtained microorganism species.The substratum of enlarged culturing is sucrose 10g/L, extractum carnis 3g/L, peptone 3g/L, (NH
4)
2sO
40.8g/L, MgSO
40.5g/L, K
2hPO
40.4g/L, KH
2pO
40.3g/L, CaCl
20.2g/L, surplus is water; Fermention medium is sucrose 20g/L, glucose 10g/L, extractum carnis 5g/L, peptone 5g/L, corn steep liquor 8g/L, MgSO
40.8g/L, K
2hPO
40.5g/L, KH
2pO
40.5g/L, NH
4cl0.3g/L, CaCl
20.2g/L, surplus is water.
Embodiment 3
Take Pseudomonas aeruginosa at 8: 4: 3: 2: 1: 0.5: 0.5 by weight ratio, bacillus cereus, Bacillus subtillis, microbacterium, intestinal bacteria, pseudomonas putida, Bacillus licheniformis, mixing, first be inoculated in culture tank with the weightmeasurement ratio of 3%, enlarged culturing 16h, culture tank rotating speed 180rpm, culture temperature 32 DEG C, be inoculated in fermentor tank with the weightmeasurement ratio of 6% after controlling oxygen level 0.2mmol/L in culturing process, fermentation culture 36h, fermentor tank rotating speed 50rpm, leavening temperature 32 DEG C, oxygen level 0.2mmol/L is controlled in fermenting process, the fermented liquid of obtained microorganism species.Enlarged culturing base is sucrose 8g/L, extractum carnis 2g/L, peptone 2g/L, (NH
4)
2sO
40.6g/L, MgSO
40.4g/L, K
2hPO
40.3g/L, KH
2pO
40.2g/L, CaCl
20.1g/L, surplus is water; Fermention medium is sucrose 15g/L, glucose 8g/L, extractum carnis 4g/L, peptone 4g/L, corn steep liquor 7g/L, MgSO
40.7g/L, K
2hPO
40.4g/L, KH
2pO
40.3g/L, NH
4cl0.2g/L, CaCl
20.2g/L, surplus is water.
Embodiment 4
Take Pseudomonas aeruginosa, bacillus cereus, Bacillus subtillis, microbacterium, intestinal bacteria, pseudomonas putida, Bacillus licheniformis at 12: 7: 8: 5: 3: 0.8: 0.7 by weight ratio, mixing, first be inoculated in culture tank with the weightmeasurement ratio of 3%, enlarged culturing 16h, culture tank rotating speed 180rpm, culture temperature 32 DEG C, controls oxygen level 0.2mmol/L in culturing process; After with 6% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 36h, fermentor tank rotating speed 50rpm, leavening temperature 32 DEG C, controls oxygen level 0.2mmol/L in fermenting process, the fermented liquid of obtained microorganism species.Enlarged culturing base is sucrose 8g/L, extractum carnis 2g/L, peptone 2g/L, (NH
4)
2sO
40.6g/L, MgSO
40.4g/L, K
2hPO
40.3g/L, KH
2pO
40.2g/L, CaCl
20.1g/L, surplus is water; Fermention medium is sucrose 15g/L, glucose 8g/L, extractum carnis 4g/L, peptone 4g/L, corn steep liquor 7g/L, MgSO
40.7g/L, K
2hPO
40.4g/L, KH
2pO
40.3g/L, NH
4cl0.2g/L, CaCl
20.2g/L, surplus is water.
Embodiment 5
Take Pseudomonas aeruginosa, bacillus cereus, Bacillus subtillis, microbacterium, intestinal bacteria, pseudomonas putida, Bacillus licheniformis at 10: 6: 5: 3: 2: 0.7: 0.6 by weight ratio, mixing, first be inoculated in culture tank with the weightmeasurement ratio of 3%, enlarged culturing 16h, culture tank rotating speed 180rpm, culture temperature 32 DEG C, controls oxygen level 0.2mmol/L in culturing process; After with 6% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 36h, fermentor tank rotating speed 50rpm, leavening temperature 32 DEG C, controls oxygen level 0.2mmol/L in fermenting process, the fermented liquid of obtained microorganism species.Enlarged culturing base is sucrose 8g/L, extractum carnis 2g/L, peptone 2g/L, (NH
4)
2sO
40.6g/L, MgSO
40.4g/L, K
2hPO
40.3g/L, KH
2pO
40.2g/L, CaCl
20.1g/L, surplus is water; Fermention medium is sucrose 15g/L, glucose 8g/L, extractum carnis 4g/L, peptone 4g/L, corn steep liquor 7g/L, MgSO
40.7g/L, K
2hPO
40.4g/L, KH
2pO
40.3g/L, NH
4cl0.2g/L, CaCl
20.2g/L, surplus is water.
Embodiment 6
The volume ratio of the fermented liquid of microorganism species obtained for embodiment 3 being pressed 1: 800 mixes with injection water, and after adding the nutritive medium of 1% volume ratio, inject dolostone reservoirs rock core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 10 days; The composition of described nutritive medium comprises: glucose 15g/L, corn steep liquor 15g/L, NH
4cl0.5g/L, KH
2pO
40.5g/L, surplus is water.
Embodiment 7
The volume ratio of the fermented liquid of microorganism species obtained for embodiment 4 being pressed 1: 1200 mixes with injection water, and after adding the nutritive medium of 5% volume ratio, inject dolostone reservoirs rock core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 30 days; The composition of described nutritive medium comprises: glucose 20g/L, corn steep liquor 20g/L, NH
4cl0.8g/L, KH
2pO
40.8g/L, surplus is water.
Embodiment 8
The volume ratio of the fermented liquid of microorganism species obtained for embodiment 5 being pressed 1: 1000 mixes with injection water, and after adding the nutritive medium of 2% volume ratio, inject from cloud rock reservoir core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 15 days; The composition of described nutritive medium comprises: glucose 18g/L, corn steep liquor 18g/L, NH
4cl0.6g/, KH
2pO
40.6g/L, surplus is water.
Test example 1
The fermented liquid of the microorganism species prepared by embodiment 3-5 adds water by after the dilution proportion of 1: 1000, then adds nutritive medium (glucose 18g/L, corn steep liquor 18g/L, NH of 2% volume ratio
4cl0.6g/L, KH
2pO
40.6g/L, surplus is water) after, respectively obtained soak solution 1.-3..
Get dolostone reservoirs rock core be soaked in this soak solution 1.-3. in, airtight preservation is after 15 days, measures the changing conditions of the pore diameter of dolostone reservoirs rock core, length and porosity, the results are shown in Table 1 (n=5).
Table 1
Can obtain according to table 1 result, adopt microorganism species fermentation liquor treatment dolostone reservoirs rock core of the present invention, the pore diameter of rock core, length and porosity size can be increased.
Test example 2
Soak solution prepared by test example 1 1.-3. mix with the crude oil containing dolostone reservoirs rock core by the volume ratio of 1%, measure the changing conditions of viscosity of crude and oil water interfacial tension under room temperature, the results are shown in Table 2 (n=5).
Table 2
Adopt microorganism species fermentation liquor treatment of the present invention to contain the crude oil of dolostone reservoirs rock core, viscosity of crude and oil water interfacial tension can be reduced.
Claims (10)
1. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio, it is characterized in that, described microorganism species comprises Pseudomonas aeruginosa (Pseudomonasaeruginosa), bacillus cereus (Bacilluscereus), Bacillus subtillis (Bacillussubtilis), microbacterium (Microbacteriumsp.), intestinal bacteria (Escherichiacoli), pseudomonas putida (Pseudomonasputida) and Bacillus licheniformis (Bacilluslicheniformis).
2. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 1, it is characterized in that, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is (5 ~ 15): (2 ~ 10): (1 ~ 10): (0.5 ~ 8): (0.5 ~ 5): (0.3 ~ 1): (0.3 ~ 1).
3. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 1, it is characterized in that, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is (8 ~ 12): (4 ~ 7): (3 ~ 8): (2 ~ 5): (1 ~ 3): (0.5 ~ 0.8): (0.5 ~ 0.7).
4. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 1, it is characterized in that, Pseudomonas aeruginosa in described microorganism species: bacillus cereus: Bacillus subtillis: microbacterium: intestinal bacteria: pseudomonas putida: the weight proportion of Bacillus licheniformis is 10: 6: 5: 3: 2: 0.7: 0.6.
5. the microorganism species that utilizes according to claim 2-4 any one improves the method for dolostone reservoirs Crude Oil recovery ratio, it is characterized in that, after described microorganism species is composite by weight ratio, first connect in seed culture tank with the weightmeasurement ratio of 2 ~ 4%, enlarged culturing 12 ~ 24h, culture tank rotating speed 150 ~ 200rpm, culture temperature 30 ~ 35 DEG C, controls oxygen level 0.1 ~ 0.5mmol/L in culturing process; After with 5 ~ 8% weightmeasurement ratio be inoculated in fermentor tank, fermentation culture 2448h, fermentor tank rotating speed 30 ~ 80rpm, leavening temperature 30 ~ 35 DEG C, controls oxygen level 0.1 ~ 0.5mmol/L in fermenting process, the fermented liquid of obtained microorganism species.
6. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 5, it is characterized in that, the substratum of described enlarged culturing is sucrose 5 ~ 10g/L, extractum carnis 1 ~ 3g/L, peptone 1 ~ 3g/L, (NH
4)
2sO
40.5 ~ 0.8g/L, MgSO
40.3 ~ 0.5g/L, K
2hPO
40.2 ~ 0.4g/L, KH
2pO
40.1 ~ 0.3g/L, CaCl
20.1 ~ 0.2g/L, surplus is water.
7. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 5, it is characterized in that, the fermention medium of described microorganism species is sucrose 10 ~ 20g/L, glucose 5 ~ 10g/L, extractum carnis 3 ~ 5g/L, peptone 3 ~ 5g/L, corn steep liquor 6 ~ 8g/L, MgSO
40.5 ~ 0.8g/L, K
2hPO
40.2 ~ 0.5g/L, KH
2pO
40.2 ~ 0.5g/L, NH
4cl0.1 ~ 0.3g/L, CaCl
20.1 ~ 0.2g/L, surplus is water.
8. the microorganism species that utilizes according to claim 5-7 any one improves the method for dolostone reservoirs Crude Oil recovery ratio, by the fermented liquid of obtained microorganism species by 1: the volume ratio of (800 ~ 1200) mixes with injection water, and after adding the nutritive medium of 1 ~ 5% volume ratio, inject dolostone reservoirs rock core, microorganism species to be implanted and the effect of dolostone reservoirs rock core carried out oil recovering after 10 ~ 30 days.
9. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 8, it is characterized in that, the composition of described nutritive medium comprises: glucose 15 ~ 20g/L, corn steep liquor 15 ~ 20g/L, NH
4cl0.5 ~ 0.8g/L, KH
2pO
40.5 ~ 0.8g/L, surplus is water.
10. the method utilizing microorganism species to improve dolostone reservoirs Crude Oil recovery ratio according to claim 8, it is characterized in that, the composition of described nutritive medium comprises: glucose 18g/L, corn steep liquor 18g/L, NH
4cl0.6g/L, KH
2pO
40.6g/L, surplus is water.
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| CN110761758A (en) * | 2018-07-26 | 2020-02-07 | 中国石油大学(北京) | Method for modifying oil and gas reservoir by using silicate bacteria |
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