CN102926728A - Indigenous microorganism activation and exogenous microorganism intensified oil production method in offshore oilfield - Google Patents
Indigenous microorganism activation and exogenous microorganism intensified oil production method in offshore oilfield Download PDFInfo
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Abstract
The invention relates to an indigenous microorganism activation and exogenous microorganism intensified oil production method in an offshore oilfield, which belongs to the technical field of producible oil index improvement, and includes the following steps: firstly, preparation of exogenous microorganism bacterial liquor: beneficial microorganisms separated from an oil deposit stratum are subjected to enrichment culture; secondly, preparation of indigenous microorganism activators: the mass percent of components are: 0.25%-0.5% byproduct molasses in sugar industry, 0.1%-0.2% byproduct corn steep liquor of corn starch, 0.2%-0.4% sodium nitrate and 0.1%-0.3% phosphate; and thirdly, underground activation of an indigenous microflora: the following stages are included: the first stage, exogenous microorganism bacterial solution is injected to improve amount of functional microorganisms and provide nutrition substrates for growth of anaerobic bacteria, and the second stage, indigenous microorganism activators are injected in the anaerobic part of a stratum to improve the oil recovery efficiency. The method has the advantages of simplicity in operation, strong suitability, no harm to an oil-bearing sand, zero pollution, improved recovery efficiency and the like.
Description
Technical field
The invention belongs to and improve the oil recovery factor technical field, particularly relate to a kind of for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method.
Background technology
At present, microorganism is improved recovery efficiency technique and is divided into two kinds of inoculating microbe oil recovery technique and endogenous microbes oil recovery techniques.The endogenous microbes oil recovery technique is directly to utilize the microbiologic population that has existed in the oil reservoir, by adding the nutrition activator, activate the useful endogenous microbes group in the oil reservoir, the oil field, land is generally by injecting a certain amount of air in oil reservoir, improve the activation amount of functional microbial, but at sea in this particular surroundings in oil field, there is very large security hidden trouble in the injection of air.Links all exists the danger of flammable mixtures blast in the air injection process, this mainly is to contain oxygen because inject air, oxygen and crude oil are in oil reservoir generation oxidation reaction, consume partial oxidation, but in the incomplete situation of oxidation reaction, light hydrocarbon component in the stratum will form the Combination explosion gas with oxygen, when the concentration of gaseous mixture reaches explosive range, and the accident of will blasting under certain condition; Simultaneously, air macropore in oil reservoir is advanced by leaps and bounds, and is also very easily explosion caused.In case the accident of blasting, comprehensive destruction of the discarded and gas injection pipeline of producing well and Injection Well will directly be caused, What is more will cause that blowout causes larger personnel's property loss, so carrying out microbe oil production, offshore oilfield can not inject air, exist the endogenous microbes oil displacement efficiency to reduce, the technical problem such as oil displacement efficiency is poor.
Summary of the invention
The present invention provides a kind of for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method for solving the technical problem that exists in the known technology.
The object of the invention provides a kind ofly to be had simple to operately, and strong adaptability can remedy the deficiency that offshore oilfield can not air injection, and do not damage oil reservoir, pollution-free, the endogenous microbes of the characteristics such as it is remarkable to improve recovery ratio, applied widely activates and inoculating microbe intensified oil reduction method.
The present invention is used for the offshore oilfield endogenous microbes and activates and inoculating microbe intensified oil reduction method the process that comprises:
1) preparation of external source bacterium liquid
The beneficial microbe that will be separated to from reservoir formation is bred cultivation on the ground, and thalline is reached greater than 10
8Individual/ml; Surface tension is lower than 23mN/m; Interfacial tension is lower than 3mN/m; Volatile fatty acid (VFA) is higher than 33mmol/L.
2) preparation of interior activator
Add the necessary nutrition that the activator purpose provides growth of microorganism, just can be in oil reservoir the in-place activation endogenous microbes, owing to be in anaerobic environment in the oil reservoir, the design of activator mainly is for facultative anaerobe in the oil reservoir and anaerobic bacteria, by this two quasi-microorganism of activator, improve their population advantages, realize that oil pool microorganisms improves the effect of waterflood efficiency.This technology is directly utilized the useful endogenous microbes group in the oil reservoir, has stronger reservoir media compliance; Because having avoided bacterial screening and bacterium liquid fermentation flow process, cost is cheaper.
Activator is comprised of following composition and amount:
Activator is by the sugared industry byproduct molasses of 0.25%-0.5% weight and the accessory substance corn steep liquor of 0.1%-0.2% weight cornstarch, and the sodium nitrate and the 0.1-0.3% phosphate that add 0.2-0.4% form.
The selection of oil reservoir answers satisfied temperature less than 80 ℃, and formation water salinity is less than 100g/L, and oil pool microorganisms quantity is greater than 10
2Individual/mL, the condition of waterflooding extraction more than 2 years.
Endogenous microbes group can be divided into two stages in underground activation:
First stage is to contain water injection well and the near wellbore zone thereof of oxygen than horn of plenty, and is general by replenishing a certain amount of air, to activate aerobic flora, make its produce a large amount of metabolites for the next stage anaerobic bacteria growth the nutrition substrate is provided.But because the limitation of offshore oilfield can not be replenished the injection air, so adopt external source to inject the deficiency that bacterium liquid remedies this stage.The injection of external source bacterium liquid has not only increased the quantity of a large amount of functional microbials, and carries a large amount of useful metabolites in the stratum, as the activation of oil reservoir deep anaerobic bacteria, the nutrition substrate that activates or the synthetic product that is conducive to the displacement of reservoir oil.
Second stage is the anaerobism area on the stratum, and facultative anaerobe and anaerobic bacteria in the orientable activation oil reservoir of activator such as methanogen, nitrate reduction bacterium and sulfate reducing bacteria, and then are improved recovery ratio.
The present invention is a kind of for the technical scheme that the offshore oilfield endogenous microbes activates and inoculating microbe intensified oil reduction method is taked to be:
A kind of for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, be characterized in: oil production method may further comprise the steps:
(1) preparation of inoculating microbe bacterium liquid
The beneficial microbe that is separated to from reservoir formation is bred cultivation on the ground, and microbial cells is reached greater than 10
8Individual/ml; Surface tension is lower than 23mN/m; Interfacial tension is lower than 3mN/m; Volatile fatty acid is higher than 33mmol/l;
(2) preparation of endogenous microbes activator
The endogenous microbes activator is that the aqueous solution contains following component, and the each component mass percent is 0.25%-0.5% sugar industry byproduct molasses, the accessory substance corn steep liquor of 0.1%-0.2% cornstarch, the sodium nitrate of 0.2-0.4%, the phosphate of 0.1-0.3%;
(3) activate under the endogenous microbes locality
Endogenous microbes group can be divided into two stages in underground activation:
Phase I, inoculating microbe bacterium liquid injects, and increases the quantity of a large amount of functional microbials, and carries a large amount of useful metabolites in the stratum, as the activation of oil reservoir deep anaerobic bacteria, the nutrition substrate that activates or the synthetic product that is conducive to the displacement of reservoir oil;
Second stage, the endogenous microbes activator is injected in the anaerobism area on the stratum, and facultative anaerobe and anaerobic bacteria in the orientable activation oil reservoir of endogenous microbes activator utilize the useful metabolite of microorganism to improve oil recovery factor.
The present invention is used for the activation of offshore oilfield endogenous microbes can also adopt following technical scheme with inoculating microbe intensified oil reduction method:
Described for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, be characterized in: during the preparation of inoculating microbe bacterium liquid, the beneficial microorganism kind is one or more in zymophyte, alkane degradation bacterium, acetogen, the methanogen.
Described for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, be characterized in: endogenous microbes group when underground activation, methanogen, nitrate reduction bacterium and sulfate reducing bacteria in the orientable activation oil reservoir of second stage endogenous microbes activator.
Described for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, be characterized in: the selection of oil reservoir answers satisfied temperature less than 80 ℃, formation water salinity is less than 100g/L, and oil pool microorganisms quantity is greater than 102/mL, the condition of waterflooding extraction more than 2 years.
Advantage and good effect that the present invention has are:
Be used for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method owing to adopted the brand-new technical scheme of the present invention, compared with prior art, it is clear and definite to have the mechanism of action, and design science is reasonable, without advantages such as nutritional agents wastes.Evident characteristic of the present invention is:
1, the external source bacterium liquid that injects, fully remedy the deficiency that offshore oilfield can not inject air, not only improved the quantity of functional microbial, and carry a large amount of useful metabolites, as, biosurfactant, organic acid etc., the growth and breeding that activates the stage Anaerobic bacterium for anaerobism provides nutrition substrate and the product that the displacement of reservoir oil of utilizing is arranged.
2, the activator that injects mainly is to provide nutrition for microorganism in the oil reservoir, make the growth of microorganism breeding and produce a large amount of metabolites, improve on the one hand the flowability of blowhole medium Crude Oil, strengthen washing oil and oil displacement efficiency, one side shutoff high permeability zone, enlarge the microorganism swept volume, increase its in oil reservoir with the interaction at rock/crude oil/water interface.
3, equipment needed thereby is simple, adopts traditional water filling ground installation can reach construction requirement
Description of drawings
Fig. 1 is thing mould experiment structural representation of the present invention.
Among the figure, 1-liquid header, 2-model, 3-pressure meter, 4-displacement pump.。
The specific embodiment
For further understanding summary of the invention of the present invention, Characteristic, hereby exemplify following examples, and cooperate accompanying drawing to be described in detail as follows:
Consult accompanying drawing 1.A kind of for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, its tar productivity of field use raising at sea process: in inoculating microbe bacterium liquid and activator injection oil reservoir, carry out the displacement of reservoir oil.
1. the selection of oil well:
Select oil field one block as the block that is fit to microbial oil displacement, its basic condition is as follows:
| Parameter | Certain oil field | Parameter | Certain oil field |
| Temperature | 56℃ | Density (20 ℃) | 951.0kg/m3 |
| Permeability | 1400md | Sulfur-bearing | 0.2772m% |
| Salinity | 5000mg/l | The content of wax | 7.06% |
| Moisture content | 80% | Colloid | 19.58% |
| Underground viscosity of crude | 50mPa·S | Asphalitine | 0.65% |
| Core intersection | 4-11m | Kinematic viscosity (20 ℃) | 6101.14mm 2/s |
| pH | 8 | Kinematic viscosity (50 ℃) | 454.49mm 2/s |
| Notes are adopted situation | One notes five are adopted | ? | ? |
Test block well group analysis of water
2. the preparation of external source bacterium liquid: the beneficial microbe that adopts the oil field to screen, in the fermentation tank of surface facility, for microorganism provides necessary nutrient source, make microbial growth, cell concentration reaches greater than 10
8Individual/ml; And surface tension is lower than 23mN/m; Interfacial tension is lower than 3mN/m; Volatile fatty acid (VFA) is higher than 33mmol/L.Beneficial microbe is for can produce the thalline that surfactant, emulsifier, sticky polymers, aerogenesis etc. are conducive to improve oil property.The beneficial microorganism kind mainly comprises: one or more in zymophyte, alkane degradation bacterium, acetogen, the methanogen.
3. the preparation of Inner nutrition liquid: comprise the sugared industry byproduct molasses of 0.25%-0.5% weight and the accessory substance corn steep liquor of 0.1%-0.2% weight cornstarch, the sodium nitrate and the 0.1-0.3% sodium phosphate that add 0.2-0.4% form.
4. thing mould experiment: the effect that improves oil recovery factor for measuring this research, simulated test block (reservoir temperature, degree of porosity, permeability, remaining oil saturation etc.) formation condition, selecting volume is that 300 * 45 * 25mm manually suppresses the vertical three layers of heterogeneous core of cementing rectangle and carries out physical model experiment.This experiment has been carried out three groups under 56 ℃, namely carry out respectively the oilfield injection water displacement of reservoir oil, the activator soln displacement of reservoir oil, external source bacterium liquid and add the activator soln oil displacement experiment under the analog block reservoir condition.In the rock core test, when water drive is above to moisture 95%, inject the 0.5PV injected water, the amplitude that improves recovery ratio is about 8 percentage points; Inject the 0.5PV activator soln, the amplitude that improves recovery ratio is about 19 percentage points; Inject first 0.1PV external source bacterium liquid, then inject the 0.4PV activator soln, in the mode of slug, the amplitude that improves recovery ratio is about 27 percentage points; As seen, external source bacterium liquid is combined with interior activator and is had better oil displacement efficiency.
Bacteria Detection result: detecting bacterial number in external source bacterium liquid and the interior activator rock core displacement test production fluid is 3.8*10
9Individual/ml, concentration has improved 4 orders of magnitude than the injected water rock core displacement test, more only adds the activator rock core displacement test and has improved 2 orders of magnitude, illustrates that this combination can better activate function bacterium quantity in the oil reservoir.
Experimental anaerobic result shows that anaerobic bacteria is also well activated, and concentration reaches 7.5*10 in the 15d left and right sides
8Individual/ml, compare with injected water rock core displacement test and activator rock core displacement test amount detection, improved respectively 5-6 and the 3-4 order of magnitude.The adding of external source bacterium is described, has produced in a large number the nutrition substrate that can be utilized by anaerobic bacteria, can better grow.
Above-mentioned experiment shows that microorganism can both effectively be activated under aerobic and anaerobic conditions, and activator is combined with external source bacterium liquid phase and is had good effect in illustrating.
In sum, the present embodiment endogenous microbes activates and the application in oil field at sea of inoculating microbe intensified oil reduction method, can the Effective Raise tar productivity.
Claims (4)
1. one kind is used for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, and it is characterized in that: oil production method may further comprise the steps:
(1) preparation of inoculating microbe bacterium liquid
The beneficial microbe that is separated to from reservoir formation is bred cultivation on the ground, and microbial cells is reached greater than 10
8Individual/ml; Surface tension is lower than 23mN/m; Interfacial tension is lower than 3mN/m; Volatile fatty acid is higher than 33mmol/L;
(2) preparation of endogenous microbes activator
The endogenous microbes activator is that the aqueous solution contains following component, and the each component mass percent is 0.25%-0.5% sugar industry byproduct molasses, the accessory substance corn steep liquor of 0.1%-0.2% cornstarch, the sodium nitrate of 0.2-0.4%, the phosphate of 0.1-0.3%;
(3) activate under the endogenous microbes locality
Endogenous microbes group can be divided into two stages in underground activation:
Phase I, inoculating microbe bacterium liquid injects, and increases the quantity of a large amount of functional microbials, and carries a large amount of useful metabolites in the stratum, as the activation of oil reservoir deep anaerobic bacteria, the nutrition substrate that activates or the synthetic product that is conducive to the displacement of reservoir oil;
Second stage, the endogenous microbes activator is injected in the anaerobism area on the stratum, and facultative anaerobe and anaerobic bacteria in the orientable activation oil reservoir of endogenous microbes activator utilize the useful metabolite of microorganism to improve oil recovery factor.
2. according to claim 1 for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, it is characterized in that: during the preparation of inoculating microbe bacterium liquid, the beneficial microorganism kind is one or more in zymophyte, alkane degradation bacterium, acetogen, the methanogen.
3. according to claim 1 and 2 for the activation of offshore oilfield endogenous microbes and inoculating microbe intensified oil reduction method, it is characterized in that: endogenous microbes group when underground activation, methanogen, nitrate reduction bacterium and sulfate reducing bacteria in the orientable activation oil reservoir of second stage endogenous microbes activator.
4. activate and inoculating microbe intensified oil reduction method for the offshore oilfield endogenous microbes according to claimed in claim 1, it is characterized in that: the selection of oil reservoir answers satisfied temperature less than 80 ℃, formation water salinity is less than 100g/L, oil pool microorganisms quantity is greater than 102/mL, the condition of waterflooding extraction more than 2 years.
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Cited By (13)
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|---|---|---|---|---|
| CN103527160A (en) * | 2013-09-25 | 2014-01-22 | 中国石油化工股份有限公司 | Method for activating oil pool indigenous microorganisms to generate bio-emulsifier |
| CN104152394A (en) * | 2014-06-29 | 2014-11-19 | 北京大学工学院包头研究院 | Method for directionally activating microorganisms with oil recovery functions in crude oil |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN104695919A (en) * | 2015-02-15 | 2015-06-10 | 南开大学 | Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration |
| CN105221126A (en) * | 2015-10-23 | 2016-01-06 | 中国石油化工股份有限公司 | A kind of regulate and control method of emulsification displacement of reservoir oil function bacterium oil reservoir origin |
| CN106499373A (en) * | 2016-11-15 | 2017-03-15 | 西安石油大学 | A kind of method that utilization organic solid castoff improves oil recovery factor |
| CN106834166A (en) * | 2016-12-29 | 2017-06-13 | 中国石油天然气股份有限公司 | Sulfate reducing bacteria activator and application thereof in microbial oil recovery |
| CN107558969A (en) * | 2016-07-01 | 2018-01-09 | 中国石油化工股份有限公司 | A kind of method that endogenous microbes are recovered the oil |
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| CN103527160A (en) * | 2013-09-25 | 2014-01-22 | 中国石油化工股份有限公司 | Method for activating oil pool indigenous microorganisms to generate bio-emulsifier |
| CN103527160B (en) * | 2013-09-25 | 2016-02-10 | 中国石油化工股份有限公司 | A kind of method activating reservoir endogenous micro-organisms product biological emulsifier |
| CN104152394A (en) * | 2014-06-29 | 2014-11-19 | 北京大学工学院包头研究院 | Method for directionally activating microorganisms with oil recovery functions in crude oil |
| CN104152394B (en) * | 2014-06-29 | 2016-10-19 | 北京大学工学院包头研究院 | A kind of method orienting the functional microorganism that recovers the oil in activation crude oil |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN104695919B (en) * | 2015-02-15 | 2018-02-16 | 南开大学 | A kind of method for selectively activating the main beneficial bacterium of oil reservoir and keeping high concentration |
| CN104695919A (en) * | 2015-02-15 | 2015-06-10 | 南开大学 | Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration |
| CN105221126B (en) * | 2015-10-23 | 2018-04-13 | 中国石油化工股份有限公司 | A kind of regulation and control method for emulsifying displacement of reservoir oil function bacterium oil reservoir origin |
| CN105221126A (en) * | 2015-10-23 | 2016-01-06 | 中国石油化工股份有限公司 | A kind of regulate and control method of emulsification displacement of reservoir oil function bacterium oil reservoir origin |
| CN107558969A (en) * | 2016-07-01 | 2018-01-09 | 中国石油化工股份有限公司 | A kind of method that endogenous microbes are recovered the oil |
| CN107795308A (en) * | 2016-08-30 | 2018-03-13 | 中国石油化工股份有限公司 | A kind of method for improving external source functional microorganism oil reservoir conformability |
| CN107795308B (en) * | 2016-08-30 | 2019-08-09 | 中国石油化工股份有限公司 | A method of improving external source functional microorganism oil reservoir conformability |
| CN106499373A (en) * | 2016-11-15 | 2017-03-15 | 西安石油大学 | A kind of method that utilization organic solid castoff improves oil recovery factor |
| CN106834166A (en) * | 2016-12-29 | 2017-06-13 | 中国石油天然气股份有限公司 | Sulfate reducing bacteria activator and application thereof in microbial oil recovery |
| CN106834166B (en) * | 2016-12-29 | 2019-12-10 | 中国石油天然气股份有限公司 | Sulfate reducing bacteria activator and application thereof in microbial oil recovery |
| CN109236253A (en) * | 2018-09-07 | 2019-01-18 | 中国石油大学(北京) | A kind of method of activation of microorganism oil reservoir and cross-linked polymer displacement remaining oil |
| CN110499269A (en) * | 2019-08-26 | 2019-11-26 | 北京大学 | A kind of " similar compatibility " principle instructing microorganism compatibility and its improve the medicine compatibility method that external source adds microbial function and efficiency |
| CN110593834A (en) * | 2019-10-28 | 2019-12-20 | 中国石油化工股份有限公司 | Dominant regulation and control oil displacement method for internal and external source functional bacteria |
| CN116218500A (en) * | 2021-12-06 | 2023-06-06 | 中国石油天然气股份有限公司 | Oil displacement system for high-freezing-point oil reservoir and preparation method thereof |
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Application publication date: 20130213 |