CN110593834A - Dominant regulation and control oil displacement method for internal and external source functional bacteria - Google Patents
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- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
Abstract
The invention belongs to the technical field of tertiary oil recovery, and particularly relates to an internal and external source functional bacteria domination regulation and control oil displacement method. The method specifically comprises the following steps: screening a target oil reservoir; analyzing endogenous functional microorganisms; screening exogenous functional microorganisms; regulating and controlling an oil displacement scheme by functional bacteria; and (4) carrying out field implementation. The microbial oil displacement method has the characteristics of wide oil reservoir application range, applicability to all oil reservoirs of microbial oil displacement, widening of application of microbial oil displacement, definition of important contribution of oil displacement functional bacteria in microbial oil displacement, clear regulation and control thought and good operability; meanwhile, the method has good field implementation effect, can improve the recovery ratio by more than 15 percent, has the input-output ratio of more than 1: 12 and has the validity period of more than 5 years.
Description
Technical Field
The invention belongs to the technical field of tertiary oil recovery, relates to a tertiary oil recovery method, and particularly relates to an internal and external source functional bacteria domination regulation and control oil displacement method.
Background
The microbial oil displacement technology is an environment-friendly and sustainable oil extraction technology, provides a new technical choice for further improving the recovery ratio of the oil field, and has wide application prospect. According to different strain sources, the microbial oil displacement technology is divided into an endogenous microbial oil displacement technology and an exogenous microbial oil displacement technology. The endogenous microbial oil displacement technology is to inject a nutrient system into an oil layer to activate oil reservoir microbes so as to enable the microbes to quickly grow, reproduce and metabolize to achieve the purpose of improving the crude oil recovery rate. The technology has the advantages of low cost, simple and convenient operation and strong adaptability, and is the main research direction of the existing microbial oil displacement technology.
Early researches find that abundant endogenous microbial populations exist in an oil reservoir and the oil reservoir has a material basis for implementing endogenous microbial oil displacement. The composition and the growth metabolic pathway of natural microbial communities are extremely complex, and the functional microorganisms of oil reservoirs mainly comprise hydrocarbon-decomposing bacteria, emulsifying bacteria, methane bacteria and the like. The hydrocarbon degrading bacteria can grow and metabolize by taking petroleum hydrocarbon as a carbon source and have the capacity of degrading crude oil; the emulsified bacteria are bacteria which can generate biological surface active substances and have the function of emulsifying crude oil, and the emulsified crude oil is more favorable for utilizing hydrocarbon decomposing bacteria; the methanogen is a kind of bacteria which can produce methane gas and carbon dioxide and has the function of swelling crude oil.
Indoor and field research results show that an energy transfer chain exists in an oil reservoir ecosystem, and multiple species are required to jointly execute multiple functions to complete complex biochemical processes. The hydrocarbon decomposing bacteria, the emulsifying bacteria and the methanogen have synergistic effect, can effectively degrade heavy components of crude oil, generate biosurfactant to emulsify and strip the crude oil, generate methane gas to swell and reduce the viscosity of the crude oil, and the microorganisms act on the crude oil to improve the fluidity of the crude oil and enhance the oil washing efficiency, so that the recovery ratio of the crude oil is improved.
Disclosure of Invention
The invention aims to provide an internal and external source functional bacteria dominance regulation oil displacement method aiming at the defects and shortcomings in the prior art, which is characterized by comprising the following steps:
1. screening of target reservoirs
The criteria for target reservoir screening were as follows: the oil deposit temperature is less than 100 ℃, the crude oil viscosity is less than 20000mPa.s, the formation water mineralization degree is less than 50000mg/L, and the permeability is more than 100 multiplied by 10-3μm2。
2. Endogenous functional microbial analysis
Discharging 5-10L of formation water before collecting a target oil reservoir sample on site, fully replacing air in the sterile sampler with sterile nitrogen, obtaining 2-5L of formation water sample, centrifugally collecting thalli, extracting bacterial genome, and performing gene quantitative test on hydrocarbon decomposing bacteria, emulsifying bacteria and methanogen.
3. Exogenous functional microorganism screening
If the target oil reservoir lacks the functional microorganisms, exogenous functional microorganism screening is required, and the specific steps are as follows: placing 100mL of target oil reservoir formation water into a culture bottle, respectively adding 2-8% of exogenous functional microorganism strains, adding corresponding activating agents, performing static culture at the oil reservoir temperature for 10-20d, and screening exogenous functional microorganism strains with microorganism activation and crude oil characteristics.
4. Oil displacement scheme by regulating and controlling functional bacteria
Filling a target oil reservoir core, vacuumizing, saturating formation water of the target oil reservoir, and calculating the Pore Volume (PV); dehydrating and degassing crude oil in a saturated target oil reservoir, and calculating the original oil content of a rock core; injecting formation water of a target oil reservoir from a rock core inlet end to a rock core outlet to produce a liquid with more than 90% of water, and calculating a primary water drive recovery ratio; supplementing screened microbes without exogenous functions into the rock core, injecting a nutrition system, sequentially activating and displacing oil of functional bacteria according to the sequence of energy metabolism, and determining an optimal injection scheme according to the displacement efficiency of crude oil.
5. Implementation in situ
Carrying out microbial oil displacement field implementation and tracking monitoring according to the optimized result of the exogenous bacteria enhanced microbial oil displacement regulation and control scheme; and analyzing and tracking detection results after the field implementation for 6-12 months, entering an evaluation and regulation stage after the field implementation, and judging whether the scheme needs to be adjusted according to the results.
The quantitative gene test of the hydrocarbon degrading bacteria, the emulsifying bacteria and the methanogen in the step 1 is to quantitatively analyze the number of corresponding functional genes in total DNA of oil reservoir samples by a real-time PCR instrument by respectively adopting degenerate primers of alkane monooxygenase genes related to hydrocarbon degradation, degenerate primers of protein tyrosine phosphatase genes closely related to metabolic synthesis of biological emulsifiers and degenerate primers of hydrogenase genes closely related to metabolic synthesis of methane, and if the detection result in the samples is negative, the corresponding exogenous functional bacteria are supplemented, and vice versa.
Wherein, the hydrocarbon decomposing bacteria in the step 2 refers to one of pseudomonas, serratia, bacillus, sphingomonas and arthrobacter; the emulsifying bacteria is one of Geobacillus, Rhodococcus, Achromobacter and Acinetobacter; the methanogen is one of Methanococcus, Methanothermus methanolica, and Methanosarcina methanolica.
Wherein the formula of the hydrocarbon degrading bacteria activator in the step 3 comprises 0.2-0.5 wt% of urea, 0.05-0.2 wt% of diammonium hydrogen phosphate and 0.04-0.1 wt% of yeast powder; the formula of the activating agent of the emulsifying bacteria is 0.5-0.8 wt% of cane sugar, 0.2-0.5 wt% of corn steep liquor, 0.05-0.2 wt% of dipotassium hydrogen phosphate and 0.04-0.1 wt% of magnesium sulfate; the activating agent formula of the methanogen is 0.05-0.2 wt% of sodium acetate, 0.05-0.2 wt% of peptone, 0.05-0.2 wt% of ammonium sulfate and 0.05-0.1 wt% of vitamin.
Wherein the microorganism activation and crude oil property in the step 3 areThe specific indexes are as follows: the number of the hydrocarbon degrading bacteria genes is more than 5 multiplied by 109The crude oil degradation rate of the copies/mL and the degrading bacteria is more than 80 percent; the number of emulsifying bacteria gene is more than 6 multiplied by 108copies/mL, hydrocarbon emulsification index of emulsification bacteria is more than 90%; the number of methanogen genes is more than 3 multiplied by 108The gas pressure of the copes/mL and the methanogen is more than 0.1 MPa.
Wherein, the sequential activation and oil displacement of the functional bacteria according to the sequence of energy metabolism in the step 4 means that a hydrocarbon degrading bacteria nutrition system is injected into 0.1 PV-0.5 PV at first, and the displacement is carried out until the activation ratio of the hydrocarbon degrading bacteria in the produced liquid is less than 20%; then injecting an emulsifying bacterium nutrient system of 0.02 PV-0.2 PV, and displacing until the activation ratio of the emulsifying bacterium in the produced liquid is less than 15%; then the methanogen nutrition system 0.01 PV-0.3 PV is injected for culture displacement, and the oil displacement effect of the exogenous functional bacteria for reinforcing the oil reservoir microorganisms is fully exerted.
According to the deletion conditions of hydrocarbon decomposing bacteria, emulsifying bacteria and methanogen in an oil reservoir, corresponding functional microorganisms are added, the concentration and activity of the whole functional microorganisms are improved, the microbial ecosystem is improved, the completeness of a conversion metabolic pathway from crude oil hydrocarbon to methane of the microorganisms is guaranteed, the hydrocarbon decomposing bacteria degrade heavy components of the crude oil, the emulsifying bacteria are facilitated to utilize a light hydrocarbon generation substance surfactant, and the ingestion of the hydrocarbon decomposing bacteria to the crude oil is enhanced after the crude oil is emulsified; the biosurfactant generated by the emulsifying bacteria is hydrolyzed into micromolecular acid and carbon dioxide by microorganisms, which provides a substrate for methane gas generated by methanogens, and the swelling viscosity-reducing crude oil of the methane gas is beneficial to the degradation of hydrocarbon-decomposing bacteria and the utilization of the crude oil. The synergistic effect exists among the hydrocarbon decomposing bacteria, the emulsifying bacteria and the methanogen, so that the physical property of the crude oil can be obviously improved, the fluidity of the crude oil is improved, and the displacement efficiency is improved.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the microbial oil displacement method is wide in application range, suitable for all oil reservoirs of microbial oil displacement, capable of widening application of microbial oil displacement, clear in regulation and control thought and good in operability, and important contribution of oil displacement functional bacteria in microbial oil displacement is determined.
(2) The control scheme provided by the invention closely grasps the growth and metabolism characteristics and the dynamic evolution rule of the oil displacement functional bacteria lacking the functional bacteria in the target oil reservoir, so that the internal and external microbial communities develop towards the target beneficial to microbial oil recovery, and the pertinence of field implementation is improved.
(3) The method has the advantages that the controllability is strong, compared with a complex microbial community existing in the natural environment of an oil reservoir, the exogenous functional bacteria with clear physiological and biochemical properties and a nutrition system thereof are provided, and the operability and the stability of activation culture of each exogenous functional bacteria are good.
(4) The field implementation effect is good, the enhanced recovery ratio is more than 15%, the input-output ratio is more than 1: 12, and the validity period is more than 5 years.
Detailed Description
The technical solution of the present invention will be further described with reference to the following specific examples.
Example 1
Victory oil field certain block A1The buried depth is 1032 m-1150 m, the oil reservoir temperature is 65 ℃, and the oil reservoir permeability is 790 multiplied by 10-3μm2The viscosity of crude oil is 220mPa.s, the mineralization degree of formation water is 15800mg/L, and the oil-bearing area of a test area is 0.82km2Effective thickness of 10.2m and geological reserve of 1.35 multiplied by 106t. The method for implementing microbial oil displacement in the block comprises the following specific steps:
1. screening of target reservoirs
The criteria for target reservoir screening were as follows: the oil deposit temperature is less than 100 ℃, the crude oil viscosity is less than 20000mPa.s, the formation water mineralization degree is less than 50000mg/L, and the permeability is more than 100 multiplied by 10-3μm2。
Test Block A1The oil reservoir temperature is 65 ℃, and the oil reservoir permeability is 790 multiplied by 10-3μm2The crude oil viscosity is 220mPa.s, and the formation water mineralization degree is 15800mg/L, which meets the oil reservoir screening standard of the invention.
2. Endogenous functional microbial analysis
On-site collection test block A1Discharging 5L of formation water before sampling, fully displacing air in the aseptic sampler with sterile nitrogen gas to obtain 2L of formation water sample, centrifuging to collect thallus, extracting bacterial genome, and decomposing hydrocarbonAnd (4) quantitatively testing genes of bacteria, emulsifying bacteria and methanogens. The test results are shown in Table 1, and it can be seen from Table 1 that test Block A1In the absence of hydrocarbon-decomposing bacteria, emulsifying bacteria and methanogenic bacteria are present.
Table 1 test block a1Analysis results of concentrations of different functional bacteria in sample
3. Exogenous functional microorganism screening
Test Block A1The method is characterized in that exogenous hydrocarbon-degrading bacteria are deleted, and the screening of the exogenous hydrocarbon-degrading bacteria is required, and the method comprises the following specific steps: taking 5 culture bottles, adding test blocks A respectively1The formation water of (1) is 100mL, then 2% of pseudomonas, serratia, bacillus, sphingomonas and arthrobacter are respectively inoculated, 0.2 wt% of urea, 0.05 wt% of diamine hydrogen phosphate and 0.04 wt% of yeast powder are added, and the mixture is kept still and cultured for 10d at 65 ℃, and the test results are shown in Table 2.
TABLE 2 Hydrocarbon-degrading bacteria gene concentration and degradation rate test results
As can be seen from table 2: pseudomonas hydrocarbon degrading bacteria gene concentration 6X 109The copies/mL and the crude oil degradation rate are 85 percent, and the requirements of microorganism activation and crude oil characteristic indexes are met, so the screened exogenous hydrocarbon degrading bacteria are pseudomonas strains.
4. Oil displacement scheme by regulating and controlling functional bacteria
Test Block A1Vacuumizing the core, saturating formation water of a target oil reservoir, dehydrating and degassing crude oil in the saturated target oil reservoir, and injecting the formation water of the target oil reservoir from the inlet end of the core to the outlet end of the core to obtain a produced fluid with water content of more than 90%; supplementing 0.15 PV-screened pseudomonas and nutrient system lacking hydrocarbon-decomposing bacteria in the rock core, displacing for 15d, and decomposing hydrocarbon in the produced liquidThe activation proportion of the bacterial population is 15%, and the displacement efficiency is improved by up to 5.2%; then injecting a 0.1PV emulsifying bacterium nutrition system, and after displacing for 10 days, the activating ratio of the emulsifying bacterium in the produced liquid is 12%, and the highest displacement efficiency can be improved by 6.5%; and then injecting a 0.1PV methanogen nutrition system, so that the displacement efficiency is improved by 4.5 percent at most, and the total displacement efficiency is improved by 16.2 percent. The details are shown in Table 3
TABLE 3 Hydrocarbon bacteria and endogenous microorganism control protocol optimization results
4. Implementation in situ
Utilizing pseudomonas to test the block A according to the optimized result of the scheme for strengthening the regulation and control of the endogenous microorganism by the exogenous functional microorganism1Strengthening the site implementation of oil displacement, and injecting 1600m in total3Bacteria liquid with nutrient system of 5000m3。
And (3) analyzing the effect of the field test: by 10 months end in 2019 years, the oil is increased by 0.23 multiplied by 106t, the recovery ratio is improved by 17.2 percent, the input-output ratio is 1: 13.8, the validity period is 5.6 years, and the field test effect is good.
Example 2
Victory oil field certain block G2The buried depth is 1830-1918 m, the stratum temperature is 90 ℃, and the oil reservoir permeability is 920 multiplied by 10-3μm2The salinity of formation water is 42535mg/L, the viscosity of crude oil is 290mPa.s, and the oil-bearing area of a test area is 0.53km2Effective thickness of 12.2m and geological reserve of 54.3 multiplied by 104t. The method for implementing microbial oil displacement in the block comprises the following specific steps:
1. screening of target reservoirs
The criteria for target reservoir screening were as follows: the oil deposit temperature is less than 100 ℃, the crude oil viscosity is less than 20000mPa.s, the formation water mineralization degree is less than 50000mg/L, and the permeability is more than 100 multiplied by 10-3μm2。
Test Block G2The oil reservoir temperature is 90 ℃, and the oil reservoir permeability is 920 multiplied by 10-3μm2The salinity of formation water is 42535mg/L, the viscosity of crude oil is 290mPa.s, and the invention is satisfiedAnd (5) reservoir screening standards.
2. Endogenous functional microbial analysis
On-site collection test block G2Discharging 10L of formation water before an oil reservoir sample, fully replacing air in a sterile sampler with sterile nitrogen to obtain 5L of formation water sample, centrifugally collecting thalli, extracting bacterial genome, and quantitatively testing genes of hydrocarbon-decomposing bacteria, emulsifying bacteria and methanogen, wherein the test results are shown in table 4, and a test block G can be seen from table 42Deletion of emulsifying bacteria and methanogens.
TABLE 4 test Block G2Analysis of concentration of different functional bacteria in sample
3. Exogenous functional microorganism screening
Test Block G2The method is characterized in that exogenous emulsification bacteria and methanogenic bacteria are deleted, and the exogenous emulsification bacteria and the methanogenic bacteria need to be screened, and the method comprises the following specific steps: taking 7 culture bottles, respectively adding 100mL of stratum water, respectively inoculating 5% of exogenous emulsification bacteria, namely Geobacillus, Rhodococcus, achromobacter and Acinetobacter, and adding 0.5 wt% of sucrose, 0.2 wt% of corn steep liquor, 0.05 wt% of dipotassium hydrogen phosphate and 0.04 wt% of magnesium sulfate; respectively inoculating 8% methanococcus, methanothermus and methanosarcina, adding 2 wt% of sodium acetate, 0.2 wt% of peptone, 0.1 wt% of ammonium sulfate and 0.1 wt% of vitamin, and standing and culturing at 90 ℃ for 10 days. The gene concentration of the emulsification bacterium-Geobacillus is 3 multiplied by 1010copies/mL, hydrocarbon emulsification index of 95%, methanogen-methanococcus gene number 7X 108copies/mL, M.jannaschii gas pressure 0.25 MPa. Specific results are shown in tables 5 and 6.
TABLE 5 screening of emulsion bacteria
TABLE 6 Methanobacterium screening Condition
4. Oil displacement scheme by regulating and controlling functional bacteria
Filling test block G2Vacuumizing the core, saturating formation water of a target oil reservoir, dehydrating and degassing crude oil in the saturated target oil reservoir, and injecting the formation water of the target oil reservoir from the inlet end of the core to the outlet end of the core to obtain a produced fluid with water content of more than 90%; supplementing a 0.3PV screened hydrocarbon-degrading bacterium nutrition system into the rock core, and after displacing for 16d, activating the hydrocarbon-degrading bacterium colony in the produced liquid to 18 percent, so that the displacement efficiency is improved to be as high as 5.8 percent; then 0.05PV Geobacillus and a nutrition system are injected, after the displacement is carried out for 10 days, the activation ratio of the emulsifying bacteria in the produced liquid is 13 percent, and the displacement efficiency is improved by up to 6.5 percent; and 0.15PV Methanococcus and a nutrient system are injected again, so that the displacement efficiency is improved by 4.5 percent at most, and the total displacement efficiency is improved by 17.1 percent. The details are shown in Table 7.
TABLE 7 optimization results of the regulation and control protocol for emulsifying and methanogenic bacteria and endogenous microorganisms
5. Implementation in situ
Test block G using Geobacillus and Methanococcus to enhance endogenous microorganism regulation and control scheme optimization results according to exogenous functional microorganisms2The oil reservoir is enhanced to drive oil on site, and the total injection is 5800m3Bacterial liquid, nutrient system 10500m3。
And (3) analyzing the effect of the field test: the oil increase is 1.0 multiplied by 10 according to the 10 month end in 20195t, the recovery ratio is improved by 18.5 percent, the input-output ratio is 1: 15.2, the validity period is 6.3 years, and the field test effect is good.
Claims (15)
1. The method for dominantly regulating and controlling oil displacement by internal and external functional bacteria is characterized by comprising the following steps:
(1) screening a target oil reservoir;
(2) analyzing endogenous functional microorganisms;
(3) screening exogenous functional microorganisms;
(4) regulating and controlling an oil displacement scheme by functional bacteria;
(5) and (4) carrying out field implementation.
2. The method for dominantly regulating and controlling oil displacement by internal and external functional bacteria according to claim 1, wherein the target oil deposit is screened according to the following specific screening standards: the oil deposit temperature is less than 100 ℃, the crude oil viscosity is less than 20000mPa.s, the formation water mineralization degree is less than 50000mg/L, and the permeability is more than 100 multiplied by 10-3μm2。
3. The method for dominantly regulating and controlling oil displacement by using internal and external functional bacteria according to claim 1, wherein the analysis of the internal functional microorganisms comprises the following specific steps: discharging 5-10L of formation water before collecting a target oil reservoir sample on site, fully replacing air in the sterile sampler with sterile nitrogen, obtaining 2-5L of formation water sample, centrifugally collecting thalli, extracting bacterial genome, and performing gene quantitative test on hydrocarbon decomposing bacteria, emulsifying bacteria and methanogen.
4. The method for controlling oil displacement by dominance of endogenous and exogenous functional bacteria according to claim 3, wherein the quantitative gene test of hydrocarbon-degrading bacteria, emulsifying bacteria and methanogenic bacteria comprises the steps of respectively adopting degenerate primers of alkane monooxygenase genes related to hydrocarbon degradation, degenerate primers of protein tyrosine phosphatase genes closely related to the metabolic synthesis of biological emulsifiers and degenerate primers of hydrogenase genes closely related to the metabolic synthesis of methane, and quantitatively analyzing the number of corresponding functional genes in the total DNA of microorganisms in an oil reservoir sample by a real-time PCR (polymerase chain reaction) instrument, wherein if the detection result in the sample is negative, the supplementation of corresponding exogenous functional bacteria is required, and vice versa.
5. The method for controlling oil displacement by dominance of internal and external source functional bacteria according to claim 3 or 4, wherein the hydrocarbon-decomposing bacteria is one of pseudomonas, serratia, bacillus, sphingomonas and arthrobacter.
6. The method for controlling oil displacement by dominance of internal and external source functional bacteria according to claim 3 or 4, wherein the emulsifying bacteria is one of Geobacillus, Rhodococcus, Achromobacter and Acinetobacter.
7. The method for controlling oil displacement by dominance of internal and external source functional bacteria according to claim 3 or 4, wherein the methanogen is one of methanococcus, methanothermus and methanosarcina.
8. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 5, wherein the formula of the activating agent for hydrocarbon degrading bacteria comprises 0.2-0.5 wt% of urea, 0.05-0.2 wt% of diammonium hydrogen phosphate and 0.04-0.1 wt% of yeast powder.
9. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 6, wherein the formula of the activating agent of the emulsifying bacteria is 0.5-0.8 wt% of sucrose, 0.2-0.5 wt% of corn steep liquor, 0.05-0.2 wt% of dipotassium hydrogen phosphate and 0.04-0.1 wt% of magnesium sulfate.
10. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 7, wherein the formulation of the activating agent for methanogens is 0.05-0.2 wt% of sodium acetate, 0.05-0.2 wt% of peptone, 0.05-0.2 wt% of ammonium sulfate and 0.05-0.1 wt% of vitamins.
11. The method for dominantly regulating and controlling oil displacement by using internal and external source functional bacteria according to claim 1, wherein the screening of the external source functional microorganisms comprises the following specific steps: if the target oil reservoir lacks the functional microorganisms, exogenous functional microorganism screening is required, and the specific steps are as follows: placing 100mL of target oil reservoir formation water into a culture bottle, respectively adding 2-8% of exogenous functional microorganism strains, adding corresponding activating agents, performing static culture at the oil reservoir temperature for 10-20d, and screening exogenous functional microorganism strains with microorganism activation and crude oil characteristics.
12. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 11, wherein the specific indexes of the microorganism activation and crude oil characteristics are as follows: the number of the hydrocarbon degrading bacteria genes is more than 5 multiplied by 109The crude oil degradation rate of the copies/mL and the degrading bacteria is more than 80 percent; the number of emulsifying bacteria gene is more than 6 multiplied by 108copies/mL, hydrocarbon emulsification index of emulsification bacteria is more than 90%; the number of methanogen genes is more than 3 multiplied by 108The gas pressure of the copes/mL and the methanogen is more than 0.1 MPa.
13. The method for dominantly regulating and controlling oil displacement by using internal and external functional bacteria according to claim 1, wherein the oil displacement scheme by using functional bacteria comprises the following specific steps: filling a target oil reservoir core, vacuumizing, saturating formation water of the target oil reservoir, and calculating the Pore Volume (PV); dehydrating and degassing crude oil in a saturated target oil reservoir, and calculating the original oil content of a rock core; injecting formation water of a target oil reservoir from a rock core inlet end to a rock core outlet to produce a liquid with more than 90% of water, and calculating a primary water drive recovery ratio; supplementing screened microbes without exogenous functions into the rock core, injecting a nutrition system, sequentially activating and displacing oil of functional bacteria according to the sequence of energy metabolism, and determining an optimal injection scheme according to the displacement efficiency of crude oil.
14. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 1, wherein the sequential activation and oil displacement of the functional bacteria according to the sequence of energy metabolism means that a hydrocarbon degrading bacteria nutrient system is injected at first from 0.1PV to 0.5PV and the displacement is carried out until the activation ratio of the hydrocarbon degrading bacteria in a produced liquid is less than 20%; then injecting an emulsifying bacterium nutrient system of 0.02 PV-0.2 PV, and displacing until the activation ratio of the emulsifying bacterium in the produced liquid is less than 15%; then the methanogen nutrition system 0.01 PV-0.3 PV is injected for culture displacement, and the oil displacement effect of the exogenous functional bacteria for reinforcing the oil reservoir microorganisms is fully exerted.
15. The method for controlling oil displacement by dominance of internal and external functional bacteria according to claim 1, which is implemented on site and comprises the following specific steps: carrying out microbial oil displacement field implementation and tracking monitoring according to the optimized result of the exogenous bacteria enhanced microbial oil displacement regulation and control scheme; and analyzing and tracking detection results after the field implementation for 6-12 months, entering an evaluation and regulation stage after the field implementation, and judging whether the scheme needs to be adjusted according to the results.
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| CN114427405A (en) * | 2020-09-23 | 2022-05-03 | 中国石油化工股份有限公司 | Regulation and control method for endogenous microbial oil displacement |
| CN114427405B (en) * | 2020-09-23 | 2024-04-16 | 中国石油化工股份有限公司 | Regulation and control method for endogenous microorganism oil displacement |
| CN114058352A (en) * | 2021-10-14 | 2022-02-18 | 陕西瑞捷生物科技有限公司 | Changqing oil field dwarfism microorganism profile control and flooding technology system and special equipment thereof |
| CN114058352B (en) * | 2021-10-14 | 2023-01-31 | 陕西瑞捷生物科技有限公司 | Changqing oil field dwarfism microorganism profile control and flooding technology system and special equipment thereof |
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