CN114058352B - Changqing oil field dwarfism microorganism profile control and flooding technology system and special equipment thereof - Google Patents
Changqing oil field dwarfism microorganism profile control and flooding technology system and special equipment thereof Download PDFInfo
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Abstract
The invention discloses a Jurasia microbial profile control and flooding technical system in a Changqing oil field, wherein the microbial profile control and flooding system is formed by injecting microbial profile control and flooding liquid according to a certain procedure, and the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent; the injection steps of the microbial profile control and flooding liquid are as follows: oil displacement microbial agent front slug 0.01PV + front nutrient solution 0.01PV + degradable profile control agent 0.008PV + oil displacement microbial agent rear slug 0.006PV + rear nutrient solution 0.006PV; a special device for a Jurassic system microorganism profile control and flooding technology system in a Changqing oil field comprises a constant temperature incubator, wherein one side of the constant temperature incubator is provided with a gas cylinder, the other side of the constant temperature incubator is provided with a feeding box, and 3 groups of culture reaction kettles are arranged in the constant temperature incubator. The invention preferably selects the most suitable strain combination and injection system, reduces the damage to the oil reservoir and promotes the development step of the Jurassic stratum microbial oil displacement technology in the Changqing oil field.
Description
Technical Field
The invention relates to the technical field of microbial oil displacement systems and equipment, in particular to a Jurassic microbial oil displacement technical system in a Changqing oil field and special equipment thereof.
Background
The Changqing oil field is in the Oridos basin, wherein the Jurassic formation is a typical low-permeability formation, the geological condition is complex, and the exploitation difficulty is relatively large. The microbial oil displacement is a means for improving the recovery efficiency in tertiary oil recovery, and the principle is that a biological surfactant is added by using microbes to reduce the interfacial tension, change the wettability, degrade crude oil and the like, in the oil degradation process, the microbes are firstly metabolized to generate catabolic enzymes to decompose long carbon chains in oil, and in the metabolism process, the microbes can generate organic acids and gases (such as methane and CO) 2 Etc.), surfactants, etc. to promote the flow of crude oil and then oxidize the crude oil into small molecules for degradation purposes so that crude oil which is difficult to be exploited in the oil reservoir can be displaced.
However, the application scale of the microbial flooding in oil field development is not large, and the microbial flooding is still in the theoretical research and development process. The mechanism of action of microbial oil recovery involves many complex physiological, biochemical and physical processes and is influenced by many complex factors. The good oil displacement strain is the primary condition that the microorganism can increase the extraction rate of crude oil, so the screening method is of great importance for research. At the same time, the combination of microbial flooding with other tertiary oil recovery techniques is also a means widely used at the present stage, such as microbial flooding with CO 2 The combination of flooding, air flooding and the like achieves the purpose of further increasing the yield.
The patent CN104312561A discloses an application of a composite microorganism strain in tertiary oil recovery, a mixed fermentation broth of halobacter salina (Halobacterium salinum), brevibacillus brevis (Brevibacillus breviensis) and bacillus cereus (Bacillus cereus) is applied to tertiary oil recovery according to the weight ratio of 1. However, the microorganism species and the oil displacement mechanism are single, and the method is not suitable for the development and use of low permeability oil reservoirs, so that a compound oil displacement system mainly based on microorganisms is needed to meet the exploitation requirements of the Jurassic formation in the Changqing oil field.
Disclosure of Invention
Aiming at the problems, the invention provides a Changqing oilfield dwarfism microbial profile control and flooding technical system and special equipment thereof.
The technical scheme of the invention is as follows:
a microorganism profile control and flooding technology system of Jurasia in Changqing oil field is formed by injecting microorganism profile control and flooding liquid according to a certain procedure,
the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent;
the oil displacement microbial agent comprises a product surface activator strain, a gas production strain, an acid production strain and a degradation strain, wherein the degradable profile control agent is prepared by dissolving degradable profile control particles in a suspending agent at a mass concentration of 15%, and the degradable profile control particles are prepared by mixing grain powder acid-breaking particles and gel particles in a mass ratio of 2;
the injection steps of the microorganism profile control and flooding liquid are as follows: oil displacement microbial agent front slug 0.01PV + front nutrient solution 0.01PV + degradable profile control agent 0.008PV + oil displacement microbial agent rear slug 0.006PV + rear nutrient solution 0.006PV;
the oil displacement microbial agent pre-slug comprises the following strains in percentage by weight: 36-38% of a product surface activator strain, 32-34% of an acid-producing strain and 28-32% of a degrading strain, wherein the oil displacement microbial agent postposition slug comprises the following strains in percentage by weight: 26-28% of a product surfactant strain, 48-50% of a gas-producing strain and 22-26% of an acid-producing strain.
Furthermore, the product surfactant strain is pseudomonas with a metabolite of glycolipid biosurfactant, the gas-producing strain is methane thermophilic micrococcus which produces methane gas, the acid-producing strain is one or two of agrobacterium and bacillus, and the degrading strain is prepared from citrobacter freundii and pseudomonas aeruginosa according to a strain concentration ratio of 1. Different strains are mixed to realize different action effects, so that the oil displacement effect is better.
Furthermore, the grain size of the grain powder acid-crushing particles is 0.5-1.5mm, which is more beneficial to the mixing of the degradable profile control agent.
Furthermore, the gel particles are composed of an external coating and an inner core, the external coating is thermal swelling crosslinked resin, the inner core is silica sol, and the gel particles have the characteristics of low density, high strength and good permeability and cannot block formation pores.
Further, the suspending agent comprises, by mass, 2% of guar gum, 1% of modified starch, 0.4% of polymer with the molecular weight of 1500mg/L, and the balance of water, and can be fully mixed with the degradable profile control particles.
Further, the preposed nutrient solution comprises the following components in percentage by mass: glucose 0.8%, peptone 0.1%, yeast extract 0.08%, mgSO 4 ·7H 2 O 0.02％、K 2 HPO 4 0.5％、KH 2 PO 4 0.15％、Na 2 HPO 4 0.1 percent, the balance being water, and the pH value being 7 +/-0.5; the postposition nutrient solution comprises the following components in percentage by weight: 2% of sucrose, 0.05% of peptone, 0.05% of yeast powder, 0.05% of urea, 0.5% of ammonium sulfate, 0.5% of potassium chloride, 0.3% of sodium chloride, 0.01% of ferrous sulfate and the balance of water, wherein the pH value is 7 +/-0.5, and the proper nutrient solution components are adjusted according to different contents of the components of the pre-positioned and post-positioned strains.
A special device for a Changqing oilfield Jurasia microorganism profile control and flooding technology system comprises a constant temperature incubator, wherein one side of the constant temperature incubator is provided with a gas cylinder, the other side of the constant temperature incubator is provided with a feeding box, a plurality of culture reaction kettles are arranged in the constant temperature incubator, the gas cylinder is communicated with the top of each culture reaction kettle through a gas pipe, the bottom of each culture reaction kettle is provided with a discharge hole,
a plurality of mutually meshed crushing rollers are arranged in the feeding box, a grinding disc is arranged below the crushing rollers, a discharge chute is arranged below the grinding disc, one end of the discharge chute is communicated with one side of the upper part of one group of culture reaction kettles, an air blowing fan is arranged at the other end of the discharge chute, the grinding disc is divided into an upper disc and a lower disc, filtering holes are formed in the surfaces of the upper disc and the lower disc, a servo lifting motor is arranged at the center of the bottom of the lower disc, the output end of the servo lifting motor penetrates through the lower disc and then is connected with the center of the bottom of the upper disc, a rotating motor for controlling the rotation of the upper disc is arranged at the center of the upper part of the upper disc, a plurality of groups of arc-shaped grooves are formed in the surface of the upper disc, grinding columns which correspond to the arc-shaped grooves one by one are arranged at the upper part of the lower disc,
cultivate the reation kettle top and be equipped with the fixed plate, be equipped with the air vent on the fixed plate, fixed plate bottom center department is equipped with electronic pivot, electronic pivot lower extreme and rotation valve are connected, rotate the valve and train reation kettle and rotate and sealing connection, rotate the valve lower part and be equipped with a set of air duct of pressing close to and train the reation kettle inner wall, train the reation kettle inner wall and be equipped with a plurality of ring channels, the ring channel internal rotation inlays and is equipped with hollow air guide ring, the inside a plurality of air jets that is equipped with of air guide ring, air duct and every group air guide ring intercommunication and fixed connection, the gas vent has been seted up to cultivation reation kettle one side of rotation valve below.
Further, the constant temperature incubator outer wall is equipped with the pressure display who is used for showing the interior pressure of cultivation reation kettle, and the constant temperature incubator outer wall is equipped with temperature regulator, the gas-supply pipe top is equipped with the air-vent valve, conveniently observes and adjusts the pressure in the cultivation reation kettle.
Further, hanging wall one side and being equipped with the connecting rod, the connecting rod with the inside pressure sensor who is equipped with of blowing fan is connected, pressure sensor with be used for controlling the controller electric connection that blowing fan opened and stops, the filtration pore internal diameter of crossing of hanging wall is 3mm, and the filtration pore internal diameter of crossing of lower wall is 1.5mm, grinds when the upper disc descends, and pressure sensor receives the signal simultaneously, and the blowing fan is opened through controller control and is blown the powder after grinding to the cultivation reation kettle in by the lower silo.
Further, the crushing roller is 3 groups, the arc-shaped groove and the grinding column are 3 groups, the annular groove is 3 groups, and the air jet is 3 groups, so that the arrangement is reasonable and convenient to adjust.
The beneficial effects of the invention are:
(1) According to the microbial profile control and flooding technology system, on one hand, biological strains and nutrient solution thereof are gathered and bonded in a large pore passage of an oil reservoir stratum, and metabolic products play a biochemical role to peel off a rock oil film, so that the wettability is changed; on the other hand, the growth metabolites expanded by the microorganisms have the effects of degrading and displacing oil, and can effectively reduce the viscosity of crude oil and improve the recovery ratio of crude oil by being mixed with profile control particles, and stratum pores cannot be blocked.
(2) The microbial profile control and flooding technology system is suitable for the geological environment with high water content, low permeability and compact Jurassic system in the Changqing oil field, the most suitable strain combination and injection system are preferably selected, the damage to an oil reservoir is reduced, and the development pace of the microbial oil flooding technology of the Jurassic system stratum in the Changqing oil field is promoted.
(3) The special equipment for the microbial profile control and flooding technology system can well culture microbial flora through the culture reaction kettle, can uniformly and finely grind grain powder through the grinding disc, replaces the traditional stirring of a stirring paddle by ventilation stirring, prevents the microbial flora from being damaged, has better stirring effect, is more favorable for the operation of the whole microbial profile control and flooding technology system, has obvious effect, and is favorable for further popularization and use.
Drawings
FIG. 1 is a schematic view of the overall structure of the apparatus of the present invention;
FIG. 2 is a schematic diagram of the back side of the apparatus of the present invention;
FIG. 3 is a schematic view of the internal structure of the batch tank of the apparatus of the present invention;
FIG. 4 is a schematic view of the structure of the upper plate of the apparatus of the present invention;
FIG. 5 is a schematic view of the bottom wall of the apparatus of the present invention;
FIG. 6 is a schematic diagram of the internal structure of a culture reactor of the apparatus of the present invention;
FIG. 7 is a schematic diagram of the structure of an annular groove inside a culture reaction kettle of the device of the invention;
FIG. 8 is a schematic view of the structure of the air guide ring of the apparatus of the present invention.
The device comprises a constant-temperature incubator 1, a pressure display 11, a temperature regulator 12, a gas conveying pipe 13, a gas cylinder 2, a feeding box 3, a crushing roller 31, a discharge chute 32, an air blowing fan 33, a culture reaction kettle 4, a fixing plate 41, an electric rotating shaft 42, a rotating valve 43, an air guide pipe 44, an annular groove 45, an air guide ring 46, an air jet 47, an air outlet 48, an air outlet 49, a discharge outlet 5, a grinding disc 51, an upper disc 52, a lower disc 53, a filter hole 53, a servo lifting motor 54, a rotating motor 55, an arc-shaped groove 56, a grinding column 57 and a connecting rod 58.
Detailed Description
Example 1
A microbial profile control and flooding technology system of Jurassic system in Changqing oilfield is formed by injecting a microbial profile control and flooding liquid according to a certain procedure, wherein the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent;
the oil displacement microbial agent is prepared by compounding a product surface activator strain, a gas production strain, an acid production strain and a degradation strain; the product surfactant strain is pseudomonas of which the metabolite is a glycolipid biosurfactant, the gas-producing strain is methane thermophilic micrococcus which produces methane gas, the acid-producing strain is one or two of agrobacterium tumefaciens and bacillus, and the degrading strain is citrobacter freundii and pseudomonas aeruginosa which are prepared according to the strain concentration ratio of 1;
the degradable profile control agent is prepared by dissolving degradable profile control particles in a suspending agent at a mass concentration of 15%, wherein the degradable profile control particles are prepared by mixing grain milled particles and gel particles according to a mass ratio of 2; the gel particles consist of an external coating and an inner core, the external coating is thermal swelling cross-linked resin, and the inner core is silica sol; the suspending agent comprises 2 percent of guanidine gum, 1 percent of modified starch, 0.4 percent of polymer with the molecular weight of 1500mg/L and the balance of water according to the mass percentage;
the injection steps of the microbial profile control and flooding liquid are as follows: oil displacement microbial agent front slug 0.01PV + front nutrient solution 0.01PV + degradable profile control agent 0.008PV + oil displacement microbial agent rear slug 0.006PV + rear nutrient solution 0.006PV; the PV represents the pore volume multiple, namely the value obtained by dividing the injection amount or the extraction amount by the pore volume, and represents the injection amount or the extraction amount, and the injection amount or the extraction amount can also be directly represented by the volume, but cannot be compared among different oil reservoirs, so that the PV is used as a unit in the invention, and the PV can be more than 1 and can also be less than 1.
The oil displacement microbial agent preposed slug comprises the following strains in percentage by weight: 36% of product surface activator strain, 32% of acid-producing strain, 32% of degrading strain, and the strain composition and the ratio of the strain in the oil displacement microbial agent post-positioned slug are as follows: 26% of product surfactant strain, 48% of gas production strain and 26% of acid production strain;
the preposed nutrient solution comprises the following components in percentage by mass: glucose 0.8%, peptone 0.1%, yeast extract 0.08%, mgSO 4 ·7H 2 O 0.02％、K 2 HPO 4 0.5％、KH 2 PO 4 0.15％、Na 2 HPO 4 0.1 percent of water and the balance of pH 7; the postposition nutrient solution comprises the following components in percentage by weight: 2% of sucrose, 0.05% of peptone, 0.05% of yeast powder, 0.05% of urea, 0.5% of ammonium sulfate, 0.5% of potassium chloride, 0.3% of sodium chloride, 0.01% of ferrous sulfate and the balance of water, wherein the pH value is 7.
Example 2
This embodiment is substantially the same as embodiment 1, except that: the microbial agent pre-slug has different strain compositions and proportions:
a microbial profile control and flooding technology system of Jurasia in Changqing oil field is formed by injecting a microbial profile control and flooding liquid according to a certain procedure, wherein the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, a nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent;
the oil displacement microbial agent is prepared by compounding a product surface activator strain, a gas production strain, an acid production strain and a degradation strain; the product surfactant strain is pseudomonas with a metabolite of glycolipid biosurfactant, the gas-producing strain is methane thermophilic micrococcus generating methane gas, the acid-producing strain is bacillus, and the degrading strain is citrobacter freundii and pseudomonas aeruginosa which are prepared according to the strain concentration ratio of 1;
the degradable profile control agent is prepared by dissolving degradable profile control particles in a suspending agent at a mass concentration of 15%, wherein the degradable profile control particles are prepared by mixing grain milled particles and gel particles according to a mass ratio of 2; the gel particles consist of an external coating and an inner core, the external coating is thermal swelling cross-linked resin, and the inner core is silica sol; the suspending agent comprises 2 percent of guar gum, 1 percent of modified starch, 0.4 percent of polymer with the molecular weight of 1500mg/L and the balance of water by mass percentage;
the injection steps of the microbial profile control and flooding liquid are as follows: 0.01PV + 0.008PV + 0.006PV of postnutrient solution of microbial inoculum pre-slug;
the oil displacement microbial agent pre-slug comprises the following strains in percentage by weight: 37% of product surface activator strain, 33% of acid-producing strain, 30% of degrading strain, and the strain composition and proportion in the oil displacement microbial agent postpositional slug are as follows: 26% of product surfactant strain, 48% of gas production strain and 26% of acid production strain;
the preposed nutrient solution comprises the following components in percentage by mass: glucose 0.8%, peptone 0.1%, yeast extract 0.08%, mgSO 4 ·7H 2 O 0.02％、K 2 HPO 4 0.5％、KH 2 PO 4 0.15％、Na 2 HPO 4 0.1 percent of water and the balance of pH 7; the postposition nutrient solution comprises the following components in percentage by weight: 2% of sucrose, 0.05% of peptone, 0.05% of yeast powder, 0.05% of urea, 0.5% of ammonium sulfate, 0.5% of potassium chloride, 0.3% of sodium chloride, 0.01% of ferrous sulfate and the balance of water, wherein the pH value is 7.
Example 3
This embodiment is substantially the same as embodiment 1, except that: the compositions and the proportions of strains in the microbial agent pre-slug are different:
a microbial profile control and flooding technology system of Jurasia in Changqing oil field is formed by injecting a microbial profile control and flooding liquid according to a certain procedure, wherein the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, a nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent;
the oil displacement microbial agent is prepared by compounding a product surface activator strain, a gas production strain, an acid production strain and a degradation strain; the product surfactant strain is pseudomonas of which the metabolite is a glycolipid biosurfactant, the gas-producing strain is methane thermophilic micrococcus which produces methane gas, the acid-producing strain is prepared from agrobacterium tumefaciens and bacillus according to the strain concentration ratio of 1;
the degradable profile control agent is prepared by dissolving degradable profile control particles in a suspending agent at a mass concentration of 15%, wherein the degradable profile control particles are prepared by mixing grain flour acid-milled particles and gel particles at a mass ratio of 2; the gel particles consist of an external coating and an inner core, the external coating is thermal swelling cross-linked resin, and the inner core is silica sol; the suspending agent comprises 2 percent of guar gum, 1 percent of modified starch, 0.4 percent of polymer with the molecular weight of 1500mg/L and the balance of water by mass percentage;
the microorganism profile control injection system is as follows: the microbial inoculum front slug is 0.01PV + front nutrient solution is 0.01PV + degradable profile control agent is 0.008PV + microbial inoculum rear slug is 0.006PV + rear nutrient solution is 0.006PV;
the oil displacement microbial agent preposed slug comprises the following strains in percentage by weight: 38% of product surface activator strain, 34% of acid-producing strain, 28% of degrading strain, and the strain composition and proportion in the oil displacement microbial agent postpositional slug are as follows: 26% of product surfactant strain, 48% of gas production strain and 26% of acid production strain;
the preposed nutrient solution comprises the following components in percentage by mass: glucose 0.8%, peptone 0.1%, yeast extract 0.08%, mgSO 4 ·7H 2 O 0.02％、K 2 HPO 4 0.5％、KH 2 PO 4 0.15％、Na 2 HPO 4 0.1 percent, the balance being water, and the pH value being 7; the postposition nutrient solution comprises the following components in percentage by weight: 2% of sucrose, 0.05% of peptone, 0.05% of yeast powder, 0.05% of urea, 0.5% of ammonium sulfate, 0.5% of potassium chloride, 0.3% of sodium chloride, 0.01% of ferrous sulfate and the balance of water, wherein the pH value is 7.
Example 4
This embodiment is substantially the same as embodiment 1, except that: the post-positioned slugs of the microbial inoculum have different compositions and proportions of strains.
The oil displacement microbial agent pre-slug comprises the following strains in percentage by weight: 36% of product surface activator strain, 32% of acid-producing strain, 32% of degrading strain, and the strain composition and proportion in the oil displacement microbial agent postposition slug are as follows: 27% of product surfactant strain, 49% of gas-producing strain and 24% of acid-producing strain;
example 5
This embodiment is substantially the same as embodiment 1, except that: the compositions and the proportions of strains in the post-positioned slug of the microbial inoculum are different.
The oil displacement microbial agent pre-slug comprises the following strains in percentage by weight: 36% of product surface activator strain, 32% of acid-producing strain, 32% of degrading strain, and the strain composition and proportion in the oil displacement microbial agent postposition slug are as follows: 28% of product surfactant strain, 50% of gas production strain and 22% of acid production strain;
example 6
The embodiment provides a special device for a dwarfism microorganism profile control and flooding technology system in a Changqing oil field on the basis of embodiment 1, and as shown in figures 1 and 2, the special device comprises a constant temperature incubator 1, wherein one side of the constant temperature incubator 1 is provided with a gas cylinder 2, gas in the gas cylinder 2 is nitrogen, the other side of the constant temperature incubator 1 is provided with a feeding box 3, 3 groups of culture reaction kettles 4 are arranged in the constant temperature incubator 1, the gas cylinder 2 is communicated with the tops of the culture reaction kettles 4 through a gas pipe 13, the bottoms of the culture reaction kettles 4 are provided with a discharge hole 49, the outer wall of the constant temperature incubator 1 is provided with a pressure display 11 for displaying the pressure in the culture reaction kettles 4, the outer wall of the constant temperature incubator 1 is provided with a temperature regulator 12, and a pressure regulating valve is arranged above the gas pipe 13;
as shown in fig. 3-5, 3 sets of mutually meshed pulverizing rollers 31 are arranged in the feeding box 3, a grinding disc 5 is arranged below the pulverizing rollers 31, a discharge chute 32 is arranged below the grinding disc 5, one end of the discharge chute 32 is communicated with one side of the upper part of one set of the culture reaction kettle 4, the other end of the discharge chute 32 is provided with an air blowing fan 33, the grinding disc 5 is divided into an upper disc 51 and a lower disc 52, the surfaces of the upper disc 51 and the lower disc 52 are both provided with a filtering hole 53, the center of the bottom of the lower disc 52 is provided with a servo lifting motor 54, the servo lifting motor 54 is a commercially available ASSUN servo motor, the output end of the servo lifting motor 54 penetrates through the lower disc 52 and then is connected with the center of the bottom of the upper disc 51, a rotating motor 55 used for controlling the upper disc 51 to rotate is arranged at the center of the upper part of the upper disc 51, the rotating motor 55 is a commercially available brushless direct current motor, 3 groups of arc-shaped grooves 56 are formed in the surface of the upper disc 51, grinding columns 57 which are in one-to-one correspondence with the arc-shaped grooves 56 are arranged at the upper part of the lower disc 52, a connecting rod 58 is arranged on one side of the upper disc 51, the connecting rod 58 is connected with a pressure sensor arranged inside the blowing fan 33, the pressure sensor is electrically connected with a controller used for controlling the blowing fan 33 to start and stop, the pressure sensor and the controller are of commercially available models and are adjusted to be matched with the equipment through shape structure, the inner diameter of a filtering hole 53 of the upper disc 51 is 3mm, and the inner diameter of a filtering hole 53 of the lower disc 52 is 1.5mm;
as shown in fig. 6-8, a fixing plate 41 is disposed on the top of the culture reaction vessel 4, an air vent is disposed on the fixing plate 41, an electric rotating shaft 42 is disposed at the center of the bottom of the fixing plate 41, a driving portion of the electric rotating shaft 42 is a commercially available horizontal dc speed-reducing motor, the lower end of the electric rotating shaft 42 is connected to a rotating valve 43, the rotating valve 43 is connected to the culture reaction vessel 4 in a rotating and sealing manner, a set of air ducts 44 is disposed at the lower portion of the rotating valve 43 and close to the inner wall of the culture reaction vessel 4, 3 sets of annular grooves 45 are disposed on the inner wall of the culture reaction vessel 4, a hollow air guide ring 46 is embedded in the annular groove 45 in a rotating manner, 3 sets of air nozzles 47 are disposed inside the air guide ring 46, the air ducts 44 are communicated and fixedly connected to each set of air guide ring 46, and an air outlet 48 is disposed at one side of the culture reaction vessel 4 below the rotating valve 43.
The working principle of the preparation by applying the special equipment of the microbial profile control and flooding technology system is as follows:
firstly, grain particles are put into the feeding box 3, are primarily crushed by the crushing roller 31 and then fall onto the grinding disc 5 for continuous grinding, the servo lifting motor 54 controls the upper disc 51 to do up-and-down reciprocating motion, the rotating motor 55 controls the upper disc 51 to do continuous rotation, when the upper disc 51 descends, the grinding column 57 is matched and connected with the arc-shaped groove 56, meanwhile, the grinding is carried out under the rotation of the upper disc 51, the upper disc 51 is contacted with the lower disc 52 for continuous rotary grinding, when the grain diameter of the grinding powder is less than 3mm, the grinding powder falls into the discharging groove 32 through the filtering hole 53 until the grinding column 57 moves to the tail end of the arc-shaped groove 56, at the moment, the servo lifting motor 54 controls the upper disc 51 to ascend, leave the surface of the lower disc 52, and simultaneously, the grinding column 57 is separated from the arc-shaped groove 56; meanwhile, when the upper plate 51 descends to grind, the connecting rod 58 presses the pressure sensor, the pressure sensor transmits a signal to the controller, and the controller controls the blowing fan 33 to be opened to blow the ground powder from the blanking groove 32 to the culture reaction kettle 4.
If large materials cannot fall into the lower disc 52 through the filtering holes 53 of the upper disc 51 in the crushing and grinding process, the large materials fall into the arc-shaped groove 56 in the rotation process of the upper disc 51, fall into the lower disc 52 after being ground by the grinding column 57, and then fall into the discharge chute 32 after being ground into fine particles by the upper disc 51 and the lower disc 52.
Then, the flora is cultured, the culture process needs heating and stirring, the gas cylinder 2 is opened to enable gas flow to enter a space between the fixed plate 41 and the rotary valve 43 through the gas transmission pipe 13 via the vent holes on the fixed plate 41, then enter each gas guide ring 46 via the gas guide pipe 44 and be sprayed out from the gas spraying port 47, meanwhile, the electric rotating shaft 42 drives the rotary valve 43 to rotate, and simultaneously drives the gas guide pipe 44 and the gas guide rings 46 to rotate, so that the gas flow stirring of bacterial liquid in the culture reaction kettle 4 is completed, and the gas is discharged from the gas discharging port 48.
Examples of the experiments
The reservoir recovery after the reactions in examples 1-5 was calculated and compared to the reservoir without microbial profile control, and the statistical results were as follows:
example 1: the recovery ratio is 45 percent, and the daily output of a single well is 137t/d;
example 2: the recovery rate is 48 percent, and the daily output of a single well is 150t/d;
example 3: the recovery rate is 44%, and the daily yield of a single well is 131t/d;
example 4: the recovery rate is 46 percent, and the daily output of a single well is 142t/d;
example 5: the recovery rate is 43 percent, and the daily yield of a single well is 128t/d;
comparative example: the recovery ratio is 31 percent, and the daily yield of a single well is 105t/d.
The data show that the oil reservoir recovery rate and the daily single-well yield are improved after the microorganism profile control is carried out on the microorganism profile control technical system, and the improvement effect is obvious compared with a comparative example, which shows that the microorganism profile control technical system has a good effect on the exploitation of the Jurassic formation in the Changqing oil field; the control effect obtained by the composition and the proportion of the strains in the front slug in the embodiment 2 is optimal, and the control effect obtained by the composition and the proportion of the strains in the rear slug in the embodiment 4 is optimal.
Claims (4)
1. A microorganism profile control and flooding method of Jurassic series in Changqing oil field is characterized in that the microorganism profile control and flooding method is formed by injecting microorganism profile control and flooding liquid according to a certain procedure,
the microbial profile control and flooding liquid comprises a plurality of oil displacement microbial agents with strain concentration of 5%, nutrient solution for carrying the oil displacement microbial agents and a degradable profile control agent;
the oil displacement microbial agent comprises a product surface activator strain, a gas production strain, an acid production strain and a degradation strain, the degradable profile control agent is prepared by dissolving degradable profile control particles in a suspending agent at a mass concentration of 15%, and the degradable profile control particles are prepared by mixing crushed grains and gel particles in a mass ratio of 2;
the grain crushed particles have the granularity of 0.5-1.5mm;
the gel particles consist of an external coating and an inner core, the external coating is thermal swelling cross-linked resin, and the inner core is silica sol;
the injection steps of the microorganism profile control and flooding liquid are as follows: oil displacement microbial agent front slug 0.01PV + front nutrient solution 0.01PV + degradable profile control agent 0.008PV + oil displacement microbial agent rear slug 0.006PV + rear nutrient solution 0.006PV;
the preposed nutrient solution comprises the following components in percentage by mass: glucose 0.8%, peptone 0.1%, yeast extract 0.08%, mgSO 4 ·7H 2 O 0.02%、K 2 HPO 4 0.5%、KH 2 PO 4 0.15%、Na 2 HPO 4 0.1 percent and the balance of water, and the pH value is 7 +/-0.5; the postposition nutrient solution comprises the following components in percentage by weight: 2% of sucrose, 0.05% of peptone, 0.05% of yeast powder, 0.05% of urea, 0.5% of ammonium sulfate, 0.5% of potassium chloride, 0.3% of sodium chloride, 0.01% of ferrous sulfate and the balance of water, wherein the pH value is 7 +/-0.5;
the oil displacement microbial agent pre-slug comprises the following strains in percentage by weight: 36-38% of a product surface activator strain, 32-34% of an acid-producing strain and 28-32% of a degrading strain, wherein the oil displacement microbial agent post-positioned slug comprises the following strains in percentage by weight: 26-28% of a product surfactant strain, 48-50% of a gas-producing strain and 22-26% of an acid-producing strain;
the product surfactant strain is pseudomonas with a metabolite of glycolipid biosurfactant, the gas-producing strain is methane thermophilic micrococcus which produces methane gas, the acid-producing strain is one or two of agrobacterium and bacillus, and the degrading strain is prepared from citrobacter freundii and pseudomonas aeruginosa according to the strain concentration ratio of 1;
the special equipment for the method for the dwarfism microorganism profile control and flooding in the Changqing oil field comprises a constant temperature incubator (1), wherein one side of the constant temperature incubator (1) is provided with a gas cylinder (2), the other side of the constant temperature incubator (1) is provided with a feeding box (3), a plurality of culture reaction kettles (4) are arranged in the constant temperature incubator (1), the gas cylinder (2) is communicated with the tops of the culture reaction kettles (4) through a gas conveying pipe (13), the bottoms of the culture reaction kettles (4) are provided with discharge holes (49),
a plurality of mutually meshed crushing rollers (31) are arranged in the feeding box (3), a grinding disc (5) is arranged below the crushing rollers (31), a discharge chute (32) is arranged below the grinding disc (5), one end of the discharge chute (32) is communicated with one side of the upper part of one group of culture reaction kettles (4) in the discharge chute, an air blowing fan (33) is arranged at the other end of the discharge chute (32), the grinding disc (5) is divided into an upper disc (51) and a lower disc (52), filter holes (53) are formed in the surfaces of the upper disc (51) and the lower disc (52), a servo lifting motor (54) is arranged at the center of the bottom of the lower disc (52), the output end of the servo lifting motor (54) penetrates through the lower disc (52) and then is connected with the center of the bottom of the upper disc (51), a rotating motor (55) used for controlling the rotation of the upper disc (51) is arranged at the center of the upper part of the upper disc (51), a plurality of groups of arc grooves (56) are formed in the surface of the upper disc (51), grinding columns (57) which correspond to the arc grooves (56) one by one,
cultivate reation kettle (4) top and be equipped with fixed plate (41), be equipped with the air vent on fixed plate (41), fixed plate (41) bottom center department is equipped with electronic pivot (42), electronic pivot (42) lower extreme is connected with rotation valve (43), rotation valve (43) rotate and sealing connection with cultivation reation kettle (4), and rotation valve (43) lower part is equipped with a set of air duct (44) of pressing close to cultivation reation kettle (4) inner wall, and cultivation reation kettle (4) inner wall is equipped with a plurality of ring channels (45), ring channel (45) internal rotation inlays and is equipped with hollow air guide ring (46), air guide ring (46) inside is equipped with a plurality of air jets (47), air duct (44) and every group air guide ring (46) intercommunication and fixed connection rotate cultivation reation kettle (4) one side of valve (43) below and seted up gas vent (48).
2. The method for regulating and driving the Jurassic system microorganisms in the Changqing oil field according to claim 1, wherein a pressure display (11) for displaying the pressure in the culture reaction kettle (4) is arranged on the outer wall of the constant temperature incubator (1), a temperature regulator (12) is arranged on the outer wall of the constant temperature incubator (1), and a pressure regulating valve is arranged above the gas pipe (13).
3. The method for modifying and driving the Jurassic microorganisms in the Changqing oilfield according to claim 1, wherein a connecting rod (58) is arranged on one side of the upper plate (51), the connecting rod (58) is connected with a pressure sensor arranged inside the blowing fan (33), the pressure sensor is electrically connected with a controller for controlling the starting and stopping of the blowing fan (33), the inner diameter of a filtering hole (53) of the upper plate (51) is 3mm, and the inner diameter of a filtering hole (53) of the lower plate (52) is 1.5mm.
4. The method for modifying and flooding microorganisms of the Jurassic system in the Changqing oil field is characterized in that the number of the crushing rollers (31) is 3, the number of the arc-shaped grooves (56) and the number of the grinding columns (57) are 3, the number of the annular grooves (45) is 3, and the number of the air nozzles (47) is 3.
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