CN102213088A - Microbial oil recovery method - Google Patents

Microbial oil recovery method Download PDF

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CN102213088A
CN102213088A CN201010146472XA CN201010146472A CN102213088A CN 102213088 A CN102213088 A CN 102213088A CN 201010146472X A CN201010146472X A CN 201010146472XA CN 201010146472 A CN201010146472 A CN 201010146472A CN 102213088 A CN102213088 A CN 102213088A
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degrading bacteria
petroleum hydrocarbon
aeration
bacteria
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CN201010146472XA
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吴晓磊
池昌桥
汤岳琴
郭鹏
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北京大学
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Abstract

The invention discloses a microbial oil recovery method. By the method, the crude oil production rate of a field test is improved by 12.5 percent. The microbial oil recovery method provided by the invention comprises the following steps of: 1) carrying out pure oxygen aeration on a culture solution of petroleum hydrocarbon degrading bacteria to obtain an injection solution which is subjected to the pure oxygen aeration; 2) mixing the injection solution which is subjected to the pure oxygen aeration in the step 1) and a bacteria solution which contains the petroleum hydrocarbon degrading bacteria to obtain a mixed solution; and 3) injecting the mixed solution which is obtained in the step 2) into an oil reservoir by a production well for oil recovery to obtain crude oil. The content of dissolved oxygen in the injection solution is increased in a mode of carrying out pure oxygen aeration on the injection solution in a storage tank, so the activity of aerobic and/or facultative microbes can be effectively improved, life activity such as growth metabolism of the aerobic and/or facultative microbes is quickened, the degree of hydrocarbon degradation is improved, and the crude oil production can be improved.

Description

一种微生物采油方法 A microorganism oil recovery method

技术领域 FIELD

[0001] 本发明涉及微生物采油技术领域。 [0001] Technical Field The present invention relates to microbial enhanced oil recovery. 背景技术 Background technique

[0002] 微生物采油最先由前苏联和美国有关学者提出并进行研究。 [0002] Microbial Enhanced Oil Recovery was first proposed by former Soviet and American scholars and research. 是指将地面分离培养的微生物菌液和营养液注入油层,或单纯注入营养液剂或油层内的微生物,使其在油层内生长繁殖,产生有利于提高采收率的代谢产物,以提高油田采收率的采油方法,也称微生物强化采油(Microbial Enhanced Oil Recovery)。 Refers to the isolated ground cultured microorganism and nutrient broth mixture was poured into the reservoir, or microorganisms in the nutrient solutions or pure oil injection, the oil layer grown in reproduction, generating metabolites help to improve recovery, to enhance oil oil recovery method, also known as microbial enhanced oil recovery (microbial enhanced Oil Recovery). 由于其成本低、效果好、无污染,愈来愈受到人们的重视。 Because of its low cost, effective, non-polluting, more and more people's attention.

[0003] 目前国内外公认的微生物采油生化机理有两方面,一方面是微生物本身的作用: 细菌能够利用原油生长,直接降解原油组分,改变原油的物性,改善原油的流动性;通过细菌在地层中生长繁殖形成生物量,进行地层深部堵调。 [0003] It is widely recognized as the biochemical mechanism of microbial oil production there are two, one is the role of the microorganism itself: crude bacterial growth can be utilized, directly degrade components of crude oil, the crude oil composition change, improving the fluidity of crude oil; by bacteria in the the formation of biomass growth and reproduction are formed, for deep formations - plugging. 另一方面是微生物代谢产物的作用: 微生物可以代谢产生有机酸、醇、生物表面活性剂、生物聚合物、气体(队、!12、014、0)2等)等物质,能够溶解、乳化原油,降低原油的粘度,增加地层压力和原油的流动性。 On the other hand the role of microbial metabolites: microorganism metabolism can be organic acids, alcohols, biosurfactants, biopolymers, gas (team, 12,014,0!) 2, etc.) and other substances can be dissolved, emulsified oil reducing the viscosity of crude oil, increasing mobility of the formation pressure and the crude oil. 应用微生物提高石油采收率主要有以下三种方法:(1)生物工艺法采油。 Application of microbial enhanced oil recovery are the following three methods: (1) oil biotechnology method. 以地面上发酵好的培养液中提取出胞外的代谢产物,再将这些物质的溶液注入油藏促进石油的采出。 In the ground fermented broth is extracted extracellular metabolites, then a solution of these substances is injected into the reservoir to promote oil recovery. (2)异源微生物采油。 (2) a heterologous microbial enhanced oil recovery. 细菌的孢子与所需的营养物一道注进油藏,在油藏条件下它们生长并生产出在某些方面有利于石油采出的生物产物。 Bacterial spores and nutrients required for injection into a reservoir, the reservoir under conditions in which they grow and produce beneficial in certain aspects of the biological product oil extraction. (3)本源微生物采油。 (3) indigenous microbial oil. 根据油藏微生物种类、数量和生长特性,研制相应的激活剂激活那些对石油采收率有利的微生物,从而产生大量代谢产物,增加出油量。 The microbial species reservoir, number and growth characteristics, by activating the corresponding development of those microorganisms advantageous for oil recovery, to produce a large number of metabolites, increasing the amount of oil.

[0004] 而微生物采油的关键是在油藏内营造一个适合注入的菌种或油藏内有益的本源细菌大量繁殖的特定环境,使其产生有利于增油的代谢产物。 [0004] The microbial enhanced oil recovery key is to create a beneficial environment for the origin of a particular bacterial species or injected within the reservoir blooms in the reservoir, so that metabolites in favor of increasing oil. 这就涉及到细菌的生长代谢和油藏的环境条件。 This involves the growth and metabolism of bacteria and reservoir environmental conditions. 其中注入液是决定微生物在此类生态系统中分布状况的主要因子。 Wherein the injection fluid is to determine the distribution of microorganisms in the ecosystem such main factors. 在未注水的含油层或水交替贫乏的油田中通常未发现微生物的存在,这也是油藏在各地质时期未受到影响并得以保存下来的原因。 No oily layer in water or water in oil alternating poor not normally found the presence of microorganisms, which is not affected in the reservoir geologic time and the reason is preserved. 随注入水带到油层中的氧为微生物分解石油层中的烃类物质创造了有利条件。 Injection water to the oil reservoir with oxygen to create favorable conditions for microbial decomposition of hydrocarbon oil layer. 在含氧地带氧化产生的代谢产物不但是驱油剂,随着注入水推进到油层中的无氧地带,又成为各种厌氧菌发酵性细菌、硫酸盐还原菌和甲烷产生菌等利用的基质。 Metabolites is not only an oxygen-containing oxidation zone displacing agent, with the injection of water into the reservoir in advance of the anaerobic zone, and anaerobic fermentation for a variety of bacteria, sulfate-reducing bacteria and methane bacteria use to produce matrix. 而且在自然环境中,大多数的石油烃类是在好氧条件下被降解的,这是因为许多烃类的降解需要加氧酶和分子氧,所以目前筛选出来的以石油烃为碳源的菌种大部分为好氧和/或兼氧微生物。 And in the natural environment, most of the petroleum hydrocarbons are degraded under aerobic conditions, this is because many hydrocarbon degrading oxygenase and requires molecular oxygen, so as to filter out the current petroleum hydrocarbons as carbon source most strains of aerobic and / or facultative microorganisms. 虽然也筛选出一些厌氧烃降解菌,不过普遍存在代谢速度缓慢的问题。 Although screening some anaerobic hydrocarbon degrading bacteria, but the problem is widespread metabolic rate slow. 因此,利用注入外源微生物的方法来采油基本上是利用好氧和/或兼氧微生物,其前提是要有充足的氧来保证微生物的高代谢活性。 Thus, using the method of injecting oil exogenous microbes to use substantially aerobic and / or facultative anaerobic microorganisms which have sufficient oxygen is provided to ensure the high metabolic activity of the microorganisms. 而油藏地层水中的溶解氧是非常少的,所以溶解氧的含量成为它们发酵生长的限制性因子。 Dissolved oxygen reservoir formation water is very small, so that the content of dissolved oxygen becomes the growth limiting factor for their fermentation. 然而,目前提高油藏中溶解氧含量的方法又都存在各自的问题。 However, the increase in dissolved oxygen content of the reservoir there are methods and their problems. USP5163510提出注入H202、NaC103、KClO4, NaNO3来增加油藏中溶解氧浓度的方法,但存在着药剂投加成本高、改变注入液化学性质、影响微生物生长繁殖等不利因素。 USP5163510 proposed injection H202, NaC103, KClO4, NaNO3 reservoir to increase the dissolved oxygen concentration in the method, but there are high costs pharmaceutical dosing, changing the chemical nature of the infusate, Effects of microbial growth and reproduction and other unfavorable factors. 申请号为02151230. 2题为“一种微生物采油方法”的中国专利,提出了在注入微生物溶液的同时充加空气的补氧方法。 Application No. 02151230.2 Chinese Patent entitled "A microorganism recovery methods", the proposed method of oxygenating microorganism solution while injecting air charge added. 而直接注入空气会造成气相和液相的直接分离,使得系统控制运行难度加大。 Injecting air directly cause direct separation of vapor and liquid phases, such that the system controlling the operation more difficult. USP6546962提出的采用射流的方式将空气溶解到水中来提高溶解氧浓度的方法,这种方法克服了直接注入空气带来的问题,不过也存在添加注入设备,调控压力等问题。 USP6546962 manner using jets of air made dissolved into water to increase the concentration of dissolved oxygen, which overcomes the problems caused by the direct injection of air, but there is also added the injection device, the pressure regulation problems.

发明内容 SUMMARY

[0005] 本发明的目的在于提供一种微生物采油方法,利用该方法后现场试验原油产量提高了12. 5%。 [0005] The object of the present invention is to provide a method of microbial enhanced oil recovery, the oil field test using this method increases the yield of 12.5%.

[0006] 本发明提供的微生物采油方法,包括以下步骤: [0006] The present invention provides method of microbial enhanced oil recovery, comprising the steps of:

[0007] 1)对石油烃降解菌的培养液进行纯氧曝气,得到经纯氧曝气过的注入液; [0007] 1) culture liquid petroleum hydrocarbon degrading bacteria were pure oxygen aeration, aeration obtained by pure oxygen is injected through the liquid;

[0008] 2)将步骤1)中的经纯氧曝气过的注入液与含石油烃降解菌的菌液混合,得到混合液; [0008] 2) The bacterial solution was mixed pure oxygen aeration step 1) is treated with infusate containing petroleum hydrocarbon degrading bacteria to obtain a mixture;

[0009] 3)将步骤2)得到的混合液通过生产井往油藏中注入,进行采油,得到原油。 [0009] 3) in step 2) the resulting mixture is injected into the reservoir through the production well, for oil to obtain oil.

[0010] 上述油藏的参数是油藏埋深为500-3000m、温度< 90°C、矿化度< 150000mg/L和渗透率> 0. ΙμΐΉ2。 [0010] parameter of the depth of the reservoir is a reservoir 500-3000m, temperature <90 ° C, salinity <150000mg / L and permeability> 0. ΙμΐΉ2.

[0011] 上述石油烃降解菌的培养液是能够培养所述石油烃降解菌的营养液;优选是溶剂为水、溶质为以下物质组成的溶液:糖蜜10-50g/L,尿素5-10g/L,磷酸氢二铵5-10g/L,酵母膏0. 1-0. 5g/L。 [0011] The culture liquid of the petroleum hydrocarbon degrading bacteria to be able to culture the nutrient liquid petroleum hydrocarbon degrading bacteria; preferably the solvent is water, the solute of a solution consisting of the following: molasses 10-50g / L, urea 5-10g / L, diammonium phosphate 5-10g / L, yeast extract 0. 1-0. 5g / L.

[0012] 上述经纯氧曝气过的注入液的溶解氧为近乎饱和浓度,具体可以是10_20mg/L,优选是16mg/L。 [0012] The aeration by oxygen injection through the near-saturated solution of dissolved oxygen concentration, can be particularly 10_20mg / L, preferably from 16mg / L.

[0013] 上述石油烃降解菌是好氧菌和/或兼性厌氧菌;优选是如下7种菌中的至少一禾中:IiM 单胞菌(Pseudomonas aeruginos)、芽ί包杆菌(Bacillus cereus)、不动杆菌(Acinetobacterjunii)、节杆菌(Arthrobacter picolinophilus)、黄单胞菌(Xanthomonas campestris)、明串珠菌(Leuconostoc mesenteroides)禾口棒杆菌(Corynebacterium alcanolyticum)。 [0013] The petroleum hydrocarbon degrading bacteria are aerobic and / or facultative anaerobes; 7 kinds of bacteria as follows is preferably at least one of the Wo: IiM Zymomonas mobilis (Pseudomonas aeruginos), Bud ί package coli (Bacillus cereus ), Acinetobacter (Acinetobacterjunii), Arthrobacter (Arthrobacter picolinophilus), Xanthomonas (Xanthomonas campestris), Leuconostoc (Leuconostoc mesenteroides) Wo port Corynebacterium (Corynebacterium alcanolyticum).

[0014] 上述7 种菌可以是假单胞菌(Pseudomonas aeruginosa, DSM No. : 50071)、 ^ M ^f M (Bacillus cereus, DSM No. :31)、 f 云力木干胃(Acinetobacter junii, DSM No. :6964) > 木干胃(Arthrobacter picolinophilus, DSM No. :43066) > H Ifi Ir (Xanthomonascampestris, DSM No. :3586)、明串珠菌(Leuconostoc mesenteroides, DSM No. :20484)禾口棒杆菌(Corynebacterium alcanolyticum, DSM No. :20606)。 [0014] The inoculum may be the seven Pseudomonas (Pseudomonas aeruginosa, DSM No.: 50071), ^ M ^ f M (Bacillus cereus, DSM No.: 31), f cloud force dry wood stomach (Acinetobacter junii, DSM No.: 6964)> dry wood stomach (Arthrobacter picolinophilus, DSM No.: 43066)> H Ifi Ir (Xanthomonascampestris, DSM No.: 3586), Leuconostoc (Leuconostoc mesenteroides, DSM No.: 20484) Wo opening rod bacillus (Corynebacterium alcanolyticum, DSM No.: 20606).

[0015] 上述含石油烃降解菌的菌液是将所述7种菌单独培养成IO8-IOiciCFUAiI的单菌液,然后等体积混合得到的。 [0015] The bacteria-containing petroleum hydrocarbon degrading bacteria were cultured alone is 7 IO8-IOiciCFUAiI inoculum into a single cell suspension, and then mixed with an equal volume obtained.

[0016] 上述经纯氧曝气过的注入液与所述含石油烃降解菌的菌液的体积比是1 : (0.002-0.01)。 [0016] The aeration was pure oxygen is injected through the liquid-containing broth volume ratio of petroleum hydrocarbon degrading bacteria is 1: (0.002-0.01).

[0017] 进一步,上述经纯氧曝气过的注入液与所述含石油烃降解菌的菌液的体积比是 [0017] Further, the above-described aeration by oxygen injection through the liquid-containing broth volume ratio of the petroleum hydrocarbon degrading bacteria is

1 : 0. Olo 1: 0. Olo

[0018] 上述步骤3)中的混合液的注入量具体可以是0. 05PV(PV是注入体积倍数即为所注入的体积是地层孔隙体积的倍数)。 Injection amount of the mixed solution in [0018] Step 3 above) may be particularly 0. 05PV (PV is the multiple injection volume is injected volume is a multiple of the pore volume of the formation).

[0019] 利用该方法后现场试验原油产量提高了12.5%。 After using this method [0019] Field Test crude oil production increased by 12.5%. [0020] 本发明对原有的微生物采油技术的改进涉及:通过对储存罐中注入液进行纯氧曝气的方式来增加注入液中的溶解氧的含量,可有效的提高好氧和/或兼氧微生物的活性, 加快其生长代谢等生命活动,使得烃类降解程度提高等,为后面的厌氧菌提供更多可利用的基质,以此增加原油的流动性,而微生物产生的生物聚合物,可增加水相粘度,从而提高波及系数和降低水油比。 [0020] The present invention relates to an improvement of the original MEOR: a storage tank by way of the injection liquid is pure oxygen aeration to increase the dissolved oxygen content of the injected liquid, can effectively improve the aerobic and / or Also the activity of aerobic microorganisms, metabolic accelerate the growth of living organisms, such hydrocarbons to improve the degree of degradation, to provide more substrate available for the subsequent anaerobic, thereby increasing the flow of crude oil, polymerized and biological microorganisms composition, increase the viscosity of the aqueous phase, thus improving sweep efficiency and reduce the water to oil ratio. 生长繁殖的菌体和代谢物与重金属形成的沉淀物也形成物理堵塞,起调剖作用,可进行选择性封堵以改变水的流向。 The precipitate growth and reproduction of bacteria and heavy metals metabolites formed is also formed with a physical blockage, play the role of profile, can selectively block the flow of water to change. 细菌进入水驱油层并在油层孔隙的孔喉处产生聚合物,对含水层的孔隙进行有效的封堵。 Water flooding into the reservoir and bacteria produce a polymer in the pores of the pore throat reservoir, aquifer pore plugging effectively. 同时由于所注入的混合液中的石油烃降解混合菌可以在有氧的条件下直接利用石油烃类物质,因此不需要补充有机营养物。 And because the injected mixture of petroleum degradation by mixed bacteria can directly use petroleum hydrocarbons under aerobic conditions, it is not necessary supplements organic nutrients. 这样可以最大限度地发挥微生物的生长代谢功能,提高原油产量。 This can maximize microbial growth and metabolic function, enhanced oil production. 从下面的实施例中可以发现,本发明施工简单、成本低、采油率高。 Can be found from the following embodiment, the present invention has simple construction, low cost, production rate.

具体实施方式 Detailed ways

[0021] 下面结合具体实施例对本发明作进一步说明,但本发明并不限于以下实施例。 [0021] The following embodiments in conjunction with specific embodiments of the present invention is further illustrated, but the present invention is not limited to the following embodiments.

[0022] 下述实施例中,如无特殊说明,均为常规方法。 [0022] In the following examples, Unless otherwise specified, all conventional methods.

[0023] 实施例1、本发明的微生物采油及其效果监测 [0023] Example 1, microbial enhanced oil recovery and monitoring the effect of the present invention

[0024] 一、微生物采油 [0024] First, microbial enhanced oil recovery

[0025] 在人造或天然岩心模拟的油藏条件下进行模拟驱油实验,来确定最佳的注入液溶解氧含量和石油烃降解混合菌的用量,为现场应用提供参考。 [0025] In the flooding experiment simulated the conditions of an artificial or natural core reservoir simulation, to determine the best content of dissolved oxygen infusate and the amount of mixing petroleum degradation bacteria, provide reference for field application. 现场应用的步骤如下: Field application of the following steps:

[0026] 1、选择一个适合微生物驱油的注水采油区块试验。 [0026] 1, choose a suitable test organism block oil injection flooding. 该区块的油藏条件如下所述, 油藏埋深为500-3000m ;温度< 90°C ;矿化度< 150000mg/L ;渗透率> 0. 1 μ m2的油藏。 The block reservoir conditions described below, the reservoir depth is 500-3000m; temperature <90 ° C; salinity <150000mg / L; permeability> 0. 1 μ m2 reservoir.

[0027] 2、对储存罐(压力可达2MPa)中的石油烃降解菌的培养液(溶剂是水,溶质是30g/L的糖蜜,10g/L的尿素,8g/L的磷酸氢二铵,0. 2g/L的酵母膏)进行纯氧曝气,使石油烃降解菌的培养液成为溶解氧含量是16mg/L的注入液。 [0027] 2, the culture solution storage tank (pressure up to 2MPa) of petroleum hydrocarbon degrading bacteria (the solvent is water, the solute is 30g / L of molasses, 10g / L urea, 8g / L diammonium phosphate , 0. 2g / L yeast extract) pure oxygen aeration, the culture liquid petroleum hydrocarbon degrading bacteria become dissolved oxygen content is 16mg / L of infusate.

[0028] 3、经过纯氧曝气的注入液经管道运输至泵前,与含石油烃降解菌的菌液,按体积比1 : 0.01的比例进行混合,混合液(投入量是0.05PV)再经泵注入生产井中。 [0028] 3, through the infusion fluid via the pipeline before the pump to the pure oxygen aeration, the broth containing petroleum hydrocarbon degrading bacteria, volume ratio 1: 0.01 and mixed, the mixed solution (inputs is 0.05PV) and then by pump injection production wells.

[0029] 其中,含石油烃降解菌的菌液是将假单胞菌G^seudomonas aeruginosa, DSM No.: 50071)、芽孢杆菌(Bacillus cereus, DSM No. :31)、不动杆菌(Acinetobacter junii, DSMNo. :6964)、节杆菌(Arthrobacter picolinophilus, DSM No. :43066)、黄单胞菌(Xanthomonas campestris, DSM No. :3586)、明串珠菌(Leuconostoc mesenteroides, DSM No. :20484)和棒杆菌(Corynebacterium alcanolyticum, DSM No. :20606)分别培养成菌浓为108-109CFU/ml的单菌液,然后按等体积混合制得。 [0029] wherein the bacteria-containing petroleum hydrocarbon degrading bacteria is Pseudomonas G ^ seudomonas aeruginosa, DSM No .: 50071), Bacillus (Bacillus cereus, DSM No.: 31), Acinetobacter (Acinetobacter junii , DSMNo:. 6964), Arthrobacter (Arthrobacter picolinophilus, DSM No.: 43066), Xanthomonas (Xanthomonas campestris, DSM No.: 3586), Leuconostoc (Leuconostoc mesenteroides, DSM No.: 20484) and the rod bacillus (Corynebacterium alcanolyticum, DSM No.: 20606) were cultured to a concentration of bacteria 108-109CFU / ml single cell suspension, and then prepared by mixing equal volumes.

[0030] 4、关井5-10天后重复步骤2和3,每15天检测一次受益油井的产液量、产油量、产出液中的菌浓、溶氧、PH值,与施工前进行对比,直至采油量无明显增产趋势为止。 [0030] 4. Repeat steps 5-10 shut-2 and 3 days, every 15 days benefit detecting liquid oil production, oil production, the produced fluids in the bacteria concentrated, dissolved oxygen, PH value, and before construction comparison, the amount of oil until no significant increase trend up.

[0031] 二、方法的效果 Effect [0031] Second, the method of

[0032] 通过本实施例的油井增产可以持续一年以上,与施工前相比采出液中微生物含量由103-104CFU/ml增加至105_106CFU/ml,平均日产油量由施工前的2. 4t增加到2. 7t,原油产量提高了O. 7-2.4)/2.4 = 12. 5%。 [0032] Well stimulation may be continued by the present embodiment more than one year, with the production fluid before construction microbial content increased from 103-104CFU / ml to 105_106CFU / ml compared to the average daily oil production from the pre-construction 2. 4t increased to 2. 7t, oil production increased O. 7-2.4) /2.4 = 12. 5%. 施工6个月后累计增油45t。 45t Construction cumulative increase of oil after six months.

Claims (10)

1. 一种微生物采油方法,包括以下步骤:1)对石油烃降解菌的培养液进行纯氧曝气,得到经纯氧曝气过的注入液;2)将步骤1)中的经纯氧曝气过的注入液与含石油烃降解菌的菌液混合,得到混合液;3)将步骤2)得到的混合液通过生产井往油藏中注入,进行采油,得到原油。 CLAIMS 1. A method of microbial enhanced oil recovery, comprising the steps of: 1) culture liquid petroleum hydrocarbon degrading bacteria were pure oxygen aeration, aeration obtained by pure oxygen is injected through the liquid; 2) step 1) is pure by mixed bacterial suspension was injected through the aeration and the hydrocarbon-containing petroleum degrading bacteria to obtain a mixture; 3) in step 2) the resulting mixture is injected through the production well to the reservoir, for oil to obtain oil.
2.根据权利要求1所述的方法,其特征在于:所述油藏的参数是油藏埋深为500-3000m、温度< 90"C、矿化度< 150000mg/L 和渗透率> 0. Iym2。 2. The method according to claim 1, wherein: said reservoir is a reservoir parameter depth of 500-3000m, temperature <90 "C, salinity <150000mg / L and permeability> 0. Iym2.
3.根据权利要求1或2所述的方法,其特征在于:所述石油烃降解菌的培养液是能够培养所述石油烃降解菌的营养液;优选是溶剂为水、溶质为以下物质组成的溶液:糖蜜10-50g/L,尿素5-10g/L,磷酸氢二铵5-10g/L,酵母膏0. 1-0. 5g/L。 3. The method of claim 1 or claim 2, wherein: said medium is a petroleum hydrocarbon degrading bacteria capable of culturing the petroleum hydrocarbon degrading bacteria nutrient solution; preferably the solvent is water, the solute is composed of the following substances solution: molasses 10-50g / L, urea 5-10g / L, diammonium hydrogen phosphate 5-10g / L, yeast extract 0. 1-0 5g / L.
4.根据权利要求1-3中任一所述的方法,其特征在于:所述经纯氧曝气过的注入液的溶解氧为10-20mg/L,优选是16mg/L。 4. The method according to any of claims 1-3, characterized in that: said through aeration by oxygen injection liquid dissolved oxygen 10-20mg / L, preferably from 16mg / L.
5.根据权利要求1-4中任一所述的方法,其特征在于:所述石油烃降解菌是好氧菌和/或兼性厌氧菌;优选是如下7种菌中的至少一种:假单胞菌(Pseudomonasaeruginos)、 芽抱杆菌(Bacillus cereus)、不动杆菌(Acinetobacter junii)、节杆菌(Arthrobacter picolinophilus)、黄单胞菌(Xanthomonas campestris)、明串珠菌(Leuconostoc mesenteroides)禾口棒杆菌(Corynebacterium alcanolyticum)。 5. The method according to any of claims 1-4, characterized in that: said petroleum hydrocarbon degrading bacteria are aerobic and / or facultative anaerobes; is preferably at least one of the following seven kinds of bacteria : Pseudomonas (Pseudomonasaeruginos), Bacillus spores (Bacillus cereus), Acinetobacter (Acinetobacter junii), Arthrobacter (Arthrobacter picolinophilus), Xanthomonas (Xanthomonas campestris), Leuconostoc (Leuconostoc mesenteroides) Hekou Corynebacterium (Corynebacterium alcanolyticum).
6.根据权利要求5所述的方法,其特征在于:所述7种菌是假单胞菌(Pseudomonasaeruginosa, DSM No. :50071)、芽抱杆菌(Bacillus cereus, DSM No.: 31)、 f 云力ff ® (Acinetobacter junii, DSM No. :6964) > 木干胃(Arthrobacter picolinophilus, DSM No. :43066)、HHfi胃(Xanthomonas campestris, DSM No. :3586) > 明串珠菌(Leuconostocmesenteroides, DSM No. :20484)禾口棒杆菌(Corynebacterium alcanolyticum, DSM No. :20606)。 6. The method as claimed in claim 5, wherein: the bacterium is Pseudomonas seven kinds (Pseudomonasaeruginosa, DSM No.: 50071), Bacillus spores (Bacillus cereus, DSM No .: 31), f clouds force ff ® (Acinetobacter junii, DSM No.: 6964)> dry wood stomach (Arthrobacter picolinophilus, DSM No.: 43066), HHfi stomach (Xanthomonas campestris, DSM No.: 3586)> Leuconostoc (Leuconostocmesenteroides, DSM No .: 20484) Wo port Corynebacterium (Corynebacterium alcanolyticum, DSM No.: 20606).
7.根据权利要求6所述的方法,其特征在于:所述含石油烃降解菌的菌液是将所述7 种菌单独培养成108-101(lCFU/ml的单菌液,然后等体积混合得到。 7. The method according to claim 6, wherein: said bacteria-containing petroleum hydrocarbon degrading bacteria were cultured alone is 7 inoculum into 108-101 (lCFU / ml single cell suspension, and then an equal volume of get mixed.
8.根据权利要求1-7中任一所述的方法,其特征在于:所述经纯氧曝气过的注入液与所述含石油烃降解菌的菌液的体积比是1 : (0.002-0.01)。 The method according to any one of claims 1-7, characterized in that: said through aeration by oxygen injection ratio of the liquid volume containing petroleum hydrocarbon degrading bacteria cell suspension is 1: (0.002 -0.01).
9.根据权利要求8所述的方法,其特征在于:所述经纯氧曝气过的注入液与所述含石油烃降解菌的菌液的体积比是1 : 0.01。 9. The method according to claim 8, characterized in that: said through aeration by oxygen injection volume ratio of liquid broth containing petroleum hydrocarbon degrading bacteria is 1: 0.01.
10.根据权利要求1-9中任一所述的方法,其特征在于:所述步骤3)中混合液的注入量是0. 05PV。 10. The method according to any of claims 1-9, characterized in that: said step 3) in the injection amount of the mixed solution is 0. 05PV.
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102409018A (en) * 2011-12-15 2012-04-11 西北大学 Arthrobacter simpler strain, and culture method and application thereof
CN102587875A (en) * 2012-02-22 2012-07-18 李军 Method for improving output of crude oil by utilizing synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria
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CN106121606B (en) * 2016-08-18 2018-04-10 克拉玛依新科澳石油天然气技术股份有限公司 Keep the method for implanting of microbial activity
CN107312515A (en) * 2017-06-01 2017-11-03 大庆华理生物技术有限公司 A kind of multi-element biologic compound oil displacement agent system and its injection technology
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