CN102213088A - Microbial oil recovery method - Google Patents
Microbial oil recovery method Download PDFInfo
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- CN102213088A CN102213088A CN201010146472XA CN201010146472A CN102213088A CN 102213088 A CN102213088 A CN 102213088A CN 201010146472X A CN201010146472X A CN 201010146472XA CN 201010146472 A CN201010146472 A CN 201010146472A CN 102213088 A CN102213088 A CN 102213088A
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Abstract
The invention discloses a microbial oil recovery method. By the method, the crude oil production rate of a field test is improved by 12.5 percent. The microbial oil recovery method provided by the invention comprises the following steps of: 1) carrying out pure oxygen aeration on a culture solution of petroleum hydrocarbon degrading bacteria to obtain an injection solution which is subjected to the pure oxygen aeration; 2) mixing the injection solution which is subjected to the pure oxygen aeration in the step 1) and a bacteria solution which contains the petroleum hydrocarbon degrading bacteria to obtain a mixed solution; and 3) injecting the mixed solution which is obtained in the step 2) into an oil reservoir by a production well for oil recovery to obtain crude oil. The content of dissolved oxygen in the injection solution is increased in a mode of carrying out pure oxygen aeration on the injection solution in a storage tank, so the activity of aerobic and/or facultative microbes can be effectively improved, life activity such as growth metabolism of the aerobic and/or facultative microbes is quickened, the degree of hydrocarbon degradation is improved, and the crude oil production can be improved.
Description
Technical field
The present invention relates to the microbe oil production technical field.
Background technology
Microbe oil production is proposed by the relevant scholar with the U.S. of the former Soviet Union at first and studies.Be meant surface separation cultured microorganism bacterium liquid and nutrient solution are injected oil reservoir, or inject microorganism in nutrition liquor or the oil reservoir merely, make its growth and breeding in oil reservoir, generation helps improving the metabolite of recovery ratio, to improve the oil production method of oil recovery rate, also claim microbial enhanced oil recovery (Microbial Enhanced Oil Recovery).Because its cost is low, effective, pollution-free, more and more is subject to people's attention.
At present both at home and abroad the microbe oil production Biochemical Mechanism of generally acknowledging has two aspects, is the effect of microorganism itself on the one hand: bacterium can utilize the crude oil growth, direct degrading crude oil component, and the rerum natura of change crude oil is improved the flowability of crude oil; Form biomass by bacterium growth and breeding in the stratum, carry out the stifled accent of earth formation deep.Be the effect of microbial metabolic products on the other hand: microorganism can metabolism produce organic acid, alcohol, biosurfactant, biopolymer, gas (N
2, H
2, CH
4, CO
2Deng) etc. material, can dissolve, emulsified crude oil, reduce the viscosity of crude oil, increase the flowability of strata pressure and crude oil.Using microbe improves oil recovery and mainly contains following three kinds of methods: (1) bioprocess technology method is recovered the oil.To extract the outer metabolite of born of the same parents in the good nutrient solution of ground top fermentation, the solution with these materials injects the extraction that oil reservoir promotes oil again.(2) allos microbe oil production.The spore of bacterium is annotated oil-feed with required nutrients and is hidden, and their are grown and produce the biologic that helps the oil extraction in some aspects under reservoir condition.(3) indigenous microorganism recovers the oil.According to oil pool microorganisms kind, quantity and growth characteristics, develop corresponding activator and activate those microorganisms favourable, thereby produce a large amount of metabolites oil recovery, increase oil pump capacity.
And the key of microbe oil production is to build the useful prolific specific environment of this derived bacterium in a suitable bacterial classification that injects or the oil reservoir in oil reservoir, makes its generation help increasing the metabolite of oil.This just relates to the ambient conditions of growth metabolism and the oil reservoir of bacterium.Wherein injecting liquid is the main factor of decision microorganism in this type of ecosystem distribution situation.Oil-bearing layer or water in not water filling replace the existence of not finding microorganism in the poor oil field usually, and this also is the reason of oil reservoir under each geologic(al) period is not affected and is preserved.With injecting oxygen that water takes oil reservoir to is that the hydrocarbons that microorganism is decomposed pool has been created advantage.Containing not oil displacement agent still of metabolite that oxygen area oxidation produces,, become various anaerobe fermentation bacteriums, sulfate reducing bacteria and methane again and produce the matrix that bacterium etc. utilizes along with injecting the anaerobic area that water is advanced to oil reservoir.And in natural environment, most petroleum hydrocarbon is degraded under aerobic condition, this is because the degraded of many hydro carbons needs oxygenase and molecular oxygen, thus screen at present to be that the bacterial classification of carbon source is most of with the petroleum hydrocarbon be aerobic and/or double oxygen animalcule.Though also filter out some anaerobism alkane degradation bacterium, but ubiquity accretion rate problem slowly.Therefore, utilizing the method for injecting inoculating microbe to recover the oil is to utilize aerobic and/or double oxygen animalcule basically, and its prerequisite is to have sufficient oxygen to guarantee the hypermetabolism activity of microorganism.And the dissolved oxygen in the reservoir formation water is considerably less, so the content of dissolved oxygen becomes the restricted factor of their fermentation growths.Yet all there is problem separately again in the method that improves dissolved oxygen content in the oil reservoir at present.USP5163510 proposes to inject H
2O
2, NaClO
3, KClO
4, NaNO
3Increase the method for dissolved oxygen concentration in the oil reservoir, add the cost height, change to inject liquefaction and learn character, influence unfavorable factor such as growth of microorganism breeding but exist medicament.Application number is 02151230.2 to be entitled as the Chinese patent of " a kind of microbe oil production method ", has proposed to fill the oxygen replenishing method that adds air when injecting microbial solution.Can cause gas phase and the direct of liquid phase to separate and directly inject air, make system's control operation difficulty strengthen.The mode of the employing jet that USP6546962 proposes improves the method for dissolved oxygen concentration in the water with air dissolves, and this method has overcome the problem that direct injection air brings, and adds injection device but also exist, problems such as regulation and control pressure.
Summary of the invention
The object of the present invention is to provide a kind of microbe oil production method, utilize this method after the field trial crude output improved 12.5%.
Microbe oil production method provided by the invention may further comprise the steps:
1) nutrient solution to petroleum hydrocarbon degradation bacterium carries out pure oxygen aeration, obtains the injection liquid of crossing through pure oxygen aeration;
2) the injection liquid of crossing through pure oxygen aeration in the step 1) is mixed with the bacterium liquid that contains petroleum hydrocarbon degradation bacterium, obtain mixed liquor;
3) with step 2) mixed liquor that obtains injects in oil reservoir by producing well, recovers the oil, and obtains crude oil.
The parameter of above-mentioned oil reservoir is that reservoir buried depth is 500-3000m, temperature＜90 ℃, salinity＜150000mg/L and permeability＞0.1 μ m
2
The nutrient solution of above-mentioned petroleum hydrocarbon degradation bacterium is the nutrient solution that can cultivate described petroleum hydrocarbon degradation bacterium; Preferably solvent is that water, solute are the solution that following material is formed: molasses 10-50g/L, urea 5-10g/L, diammonium hydrogen phosphate 5-10g/L, yeast extract 0.1-0.5g/L.
The dissolved oxygen of the above-mentioned injection liquid of crossing through pure oxygen aeration specifically can be 10-20mg/L for being close to saturated concentration, preferably 16mg/L.
Above-mentioned petroleum hydrocarbon degradation bacterium is aerobic bacteria and/or facultative anaerobe; At least a in preferably following 7 kinds of bacterium: pseudomonad (Pseudomonas aeruginos), bacillus (Bacillus cereus), acinetobacter calcoaceticus (Acinetobacterjunii), arthrobacterium (Arthrobacter picolinophilus), Xanthomonas campestris (Xanthomonas campestris), leukonid (Leuconostoc mesenteroides) and excellent bacillus (Corynebacterium alcanolyticum).
Above-mentioned 7 kinds of bacterium can be pseudomonad (Pseudomonas aeruginosa, DSM No.:50071), bacillus (Bacillus cereus, DSM No.:31), acinetobacter calcoaceticus (Acinetobacter junii, DSM No.:6964), arthrobacterium (Arthrobacter picolinophilus, DSM No.:43066), Xanthomonas campestris (Xanthomonascampestris, DSM No.:3586), leukonid (Leuconostoc mesenteroides, DSM No.:20484) and excellent bacillus (Corynebacterium alcanolyticum, DSM No.:20606).
The above-mentioned bacterium liquid that contains petroleum hydrocarbon degradation bacterium is that described 7 kinds of bacterium are cultivated into 10 separately
8-10
10Single bacterium liquid of CFU/ml, the equal-volume mixing obtains then.
Above-mentioned injection liquid and the described volume ratio of crossing through pure oxygen aeration that contains the bacterium liquid of petroleum hydrocarbon degradation bacterium is 1: (0.002-0.01).
Further, above-mentioned injection liquid and the described volume ratio of crossing through pure oxygen aeration that contains the bacterium liquid of petroleum hydrocarbon degradation bacterium is 1: 0.01.
The injection rate of the mixed liquor above-mentioned steps 3) specifically can be 0.05PV (PV injects the volume multiple to be the multiple that the volume that is injected is the formation pore volume).
The field trial crude output has improved 12.5% after utilizing this method.
The present invention relates to the improvement of original microbe oil production technology: the content that increases the dissolved oxygen that injects liquid by the mode of injection liquid in the holding vessel being carried out pure oxygen aeration, can effectively improve the activity of aerobic and/or double oxygen animalcule, accelerate vital movements such as its growth metabolism, make hydrocarbon degradation degree raising etc., for the anaerobic bacteria of back provides how available matrix, increase the flowability of crude oil with this, and the biopolymer of microorganisms, can increase aqueous viscosity, thereby improve sweep efficiency and reduce water-oil factor.The sediment that the thalline of growth and breeding and metabolin and heavy metal form also forms physics and stops up, and plays the profile control effect, can carry out selective shut-off to change the flow direction of water.Bacterium enters the water drive oil reservoir and produces polymer at the pore throat place of oil reservoir hole, and the hole in aquifer is carried out effective shutoff.Because the petroleum hydrocarbon degradation Mixed Microbes of the mixed liquor that is injected can directly be utilized the petroleum hydrocarbon material under the condition of aerobic, therefore do not need to replenish organic nutrient substance simultaneously.Can bring into play the microbial growth metabolic function to greatest extent like this, improve crude output.Can find that from the following examples the present invention constructs simply, cost is low, tar productivity is high.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment, but the present invention is not limited to following examples.
Among the following embodiment, if no special instructions, be conventional method.
Embodiment 1, microbe oil production of the present invention and effect monitoring thereof
One, microbe oil production
Under the reservoir condition of artificial or natural core simulation, carry out the imitation oil displacement experiment experiment, determine the best injection liquid dissolved oxygen content and the consumption of petroleum hydrocarbon degradation Mixed Microbes, for The field provides reference.The step of The field is as follows:
1, selects the water flood recovery block test of a suitable microbial oil displacement.The reservoir condition of this block is as described below, and reservoir buried depth is 500-3000m; Temperature＜90 ℃; Salinity＜150000mg/L; Permeability＞0.1 μ m
2Oil reservoir.
2, (solvent is a water to the nutrient solution of the petroleum hydrocarbon degradation bacterium in the holding vessel (pressure can reach 2MPa), solute is the molasses of 30g/L, the urea of 10g/L, the diammonium hydrogen phosphate of 8g/L, 0.2g/L yeast extract) carry out pure oxygen aeration, make the nutrient solution of petroleum hydrocarbon degradation bacterium become the injection liquid that dissolved oxygen content is 16mg/L.
3, to pump, with the bacterium liquid that contains petroleum hydrocarbon degradation bacterium, mix by 1: 0.01 by volume ratio through pipeline transport for the injection liquid of process pure oxygen aeration, and mixed liquor (input amount is 0.05PV) is gone in the producing well through infusion again.
Wherein, the bacterium liquid that contains petroleum hydrocarbon degradation bacterium is with pseudomonad (Pseudomonas aeruginosa, DSM No.:50071), bacillus (Bacillus cereus, DSM No.:31), acinetobacter calcoaceticus (Acinetobacter junii, DSMNo.:6964), arthrobacterium (Arthrobacter picolinophilus, DSM No.:43066), Xanthomonas campestris (Xanthomonas campestris, DSM No.:3586), leukonid (Leuconostoc mesenteroides, DSM No.:20484) and excellent bacillus (Corynebacterium alcanolyticum, DSM No.:20606) to cultivate into bacterium respectively dense be 10
8-10
9Single bacterium liquid of CFU/ml, it is mixed to press equal-volume then.
4, repeating step 2 and 3 after closing well 5-10 days detected that bacterium in the production fluid amount, oil production, output liquid of the oil well of once being benefited is dense in per 15 days, dissolved oxygen, pH value, with construct before compare, till oil production does not have obvious volume increase trend.
Two, the effect of method
Oil well production increasing by present embodiment can continue more than 1 year, and compared in the extraction liquid content of microorganisms by 10 before the construction
3-10
4CFU/ml increases to 10
5-10
6CFU/ml, daily average oil production is increased to 2.7t by the preceding 2.4t of construction, and crude output has improved (2.7-2.4)/2.4=12.5%.Constructing, accumulative total increases oily 45t after 6 months.
Claims (10)
1. microbe oil production method may further comprise the steps:
1) nutrient solution to petroleum hydrocarbon degradation bacterium carries out pure oxygen aeration, obtains the injection liquid of crossing through pure oxygen aeration;
2) the injection liquid of crossing through pure oxygen aeration in the step 1) is mixed with the bacterium liquid that contains petroleum hydrocarbon degradation bacterium, obtain mixed liquor;
3) with step 2) mixed liquor that obtains injects in oil reservoir by producing well, recovers the oil, and obtains crude oil.
2. method according to claim 1 is characterized in that: the parameter of described oil reservoir is that reservoir buried depth is 500-3000m, temperature＜90 ℃, salinity＜150000mg/L and permeability＞0.1 μ m
2
3. method according to claim 1 and 2 is characterized in that: the nutrient solution of described petroleum hydrocarbon degradation bacterium is the nutrient solution that can cultivate described petroleum hydrocarbon degradation bacterium; Preferably solvent is that water, solute are the solution that following material is formed: molasses 10-50g/L, urea 5-10g/L, diammonium hydrogen phosphate 5-10g/L, yeast extract 0.1-0.5g/L.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: the dissolved oxygen of the described injection liquid of crossing through pure oxygen aeration is 10-20mg/L, preferably 16mg/L.
5. according to arbitrary described method among the claim 1-4, it is characterized in that: described petroleum hydrocarbon degradation bacterium is aerobic bacteria and/or facultative anaerobe; At least a in preferably following 7 kinds of bacterium: pseudomonad (Pseudomonasaeruginos), bacillus (Bacillus cereus), acinetobacter calcoaceticus (Acinetobacter junii), arthrobacterium (Arthrobacter picolinophilus), Xanthomonas campestris (Xanthomonas campestris), leukonid (Leuconostoc mesenteroides) and excellent bacillus (Corynebacterium alcanolyticum).
6. method according to claim 5, it is characterized in that: described 7 kinds of bacterium are pseudomonad (Pseudomonasaeruginosa, DSM No.:50071), bacillus (Bacillus cereus, DSM No.:31), acinetobacter calcoaceticus (Acinetobacter junii, DSM No.:6964), arthrobacterium (Arthrobacter picolinophilus, DSM No.:43066), Xanthomonas campestris (Xanthomonas campestris, DSM No.:3586), leukonid (Leuconostocmesenteroides, DSM No.:20484) and excellent bacillus (Corynebacterium alcanolyticum, DSM No.:20606).
7. method according to claim 6 is characterized in that: the described bacterium liquid that contains petroleum hydrocarbon degradation bacterium is that described 7 kinds of bacterium are cultivated into 10 separately
8-10
10Single bacterium liquid of CFU/ml, the equal-volume mixing obtains then.
8. according to arbitrary described method among the claim 1-7, it is characterized in that: described injection liquid and the described volume ratio of crossing through pure oxygen aeration that contains the bacterium liquid of petroleum hydrocarbon degradation bacterium is 1: (0.002-0.01).
9. method according to claim 8 is characterized in that: described injection liquid and the described volume ratio of crossing through pure oxygen aeration that contains the bacterium liquid of petroleum hydrocarbon degradation bacterium is 1: 0.01.
10. according to arbitrary described method among the claim 1-9, it is characterized in that: the injection rate of mixed liquor is 0.05PV in the described step 3).
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| CN201010146472XA CN102213088A (en) | 2010-04-12 | 2010-04-12 | Microbial oil recovery method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409018A (en) * | 2011-12-15 | 2012-04-11 | 西安瑞捷生物科技有限公司 | Arthrobacter simpler strain, and culture method and application thereof |
| CN102587875A (en) * | 2012-02-22 | 2012-07-18 | 邓振山 | Method for improving output of crude oil by utilizing synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria |
| CN102851235A (en) * | 2012-06-28 | 2013-01-02 | 北京世纪金道石油技术开发有限公司 | Ureibacillus sp. and bacterial agents and application thereof |
| CN107312515A (en) * | 2017-06-01 | 2017-11-03 | 大庆华理生物技术有限公司 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
| CN106121606B (en) * | 2016-08-18 | 2018-04-10 | 克拉玛依新科澳石油天然气技术股份有限公司 | Keep the method for implanting of microbial activity |
| CN110043234A (en) * | 2019-04-04 | 2019-07-23 | 农业部沼气科学研究所 | A kind of oil sediment processing method and activation oil sediment microorganism are come the method that improves oil recovery factor |
| CN111205842A (en) * | 2020-02-13 | 2020-05-29 | 陕西文岭微生物科技有限公司 | Microbial oil production process technology for improving oil recovery ratio |
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| US5163510A (en) * | 1991-01-29 | 1992-11-17 | Den Norske Stats Oljeselskap A.S. | Method of microbial enhanced oil recovery |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409018A (en) * | 2011-12-15 | 2012-04-11 | 西安瑞捷生物科技有限公司 | Arthrobacter simpler strain, and culture method and application thereof |
| CN102587875A (en) * | 2012-02-22 | 2012-07-18 | 邓振山 | Method for improving output of crude oil by utilizing synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria |
| CN102587875B (en) * | 2012-02-22 | 2015-12-09 | 邓振山 | A kind of use improves the method for crude output containing the composite bacterial solution synergy of phosphorus decomposing and nitrogen-fixing bacteria |
| CN102851235A (en) * | 2012-06-28 | 2013-01-02 | 北京世纪金道石油技术开发有限公司 | Ureibacillus sp. and bacterial agents and application thereof |
| CN106121606B (en) * | 2016-08-18 | 2018-04-10 | 克拉玛依新科澳石油天然气技术股份有限公司 | Keep the method for implanting of microbial activity |
| CN107312515A (en) * | 2017-06-01 | 2017-11-03 | 大庆华理生物技术有限公司 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
| CN107312515B (en) * | 2017-06-01 | 2019-09-27 | 大庆华理生物技术有限公司 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
| CN110043234A (en) * | 2019-04-04 | 2019-07-23 | 农业部沼气科学研究所 | A kind of oil sediment processing method and activation oil sediment microorganism are come the method that improves oil recovery factor |
| CN111205842A (en) * | 2020-02-13 | 2020-05-29 | 陕西文岭微生物科技有限公司 | Microbial oil production process technology for improving oil recovery ratio |
| CN111205842B (en) * | 2020-02-13 | 2022-11-15 | 陕西文岭微生物科技有限公司 | Microbial oil production process technology for improving oil recovery ratio |
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Application publication date: 20111012 |