CN106121606B - Keep the method for implanting of microbial activity - Google Patents
Keep the method for implanting of microbial activity Download PDFInfo
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- CN106121606B CN106121606B CN201610684264.2A CN201610684264A CN106121606B CN 106121606 B CN106121606 B CN 106121606B CN 201610684264 A CN201610684264 A CN 201610684264A CN 106121606 B CN106121606 B CN 106121606B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 52
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 106
- 244000005700 microbiome Species 0.000 claims abstract description 75
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 68
- 235000015097 nutrients Nutrition 0.000 claims abstract description 41
- 238000002347 injection Methods 0.000 claims description 55
- 239000007924 injection Substances 0.000 claims description 55
- 238000009413 insulation Methods 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 14
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 235000013379 molasses Nutrition 0.000 claims description 6
- 235000010344 sodium nitrate Nutrition 0.000 claims description 6
- 239000004317 sodium nitrate Substances 0.000 claims description 6
- 235000019832 sodium triphosphate Nutrition 0.000 claims description 4
- 238000005829 trimerization reaction Methods 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 19
- 230000008859 change Effects 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 7
- 238000010008 shearing Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000003921 oil Substances 0.000 description 12
- 239000010779 crude oil Substances 0.000 description 9
- 238000005457 optimization Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 231100000517 death Toxicity 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000002906 microbiologic effect Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000003462 zymogenic effect Effects 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mining & Mineral Resources (AREA)
- Geology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Fluid Mechanics (AREA)
- Physics & Mathematics (AREA)
- Geochemistry & Mineralogy (AREA)
- Environmental & Geological Engineering (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to microbe oil production method technical field, is a kind of method for implanting for keeping microbial activity, it is carried out as follows：The first step, microbial inoculum mixed liquor is obtained after indigenous microorganism agent is mixed with microbial nutrient solution.The method for implanting of the present invention for keeping microbial activity is in application process, when indigenous microorganism agent crosses pump, the shearing force that indigenous microorganism agent is subject to substantially reduces, and it can adapt to the change of pump pressure, so as to reduce the casualty rate of indigenous microorganism agent；Simultaneously, before microbial inoculum mixed liquor injects into well, because the holding temperature of indigenous microorganism agent is suitable with bottom hole temperature (BHT), and indigenous microorganism agent has been activated, therefore, when the environment that shaft bottom can be rapidly adapted to according to the indigenous microorganism agent that injects into well of method for implanting of holding microbial activity of the present invention, the activity of the indigenous microorganism agent can be kept.
Description
Technical field
The present invention relates to microbe oil production method technical field, is a kind of method for implanting for keeping microbial activity.
Background technology
Microbial Enhanced Oil Recovery research indoors obtains very big success, and each elephant is also carrying out guiding scene examination
Test, do not promote the use of on a large scale all the time, main cause is to be difficult to keep the survival and growth of strain in the earth formation, and microorganism is adopted
The strain that oil tech uses typically is cultivated in strain factory and completed, canned to make-up station or job site and microbial nutrient solution
Mix according to a certain percentage, inject pit shaft using pump truck or pump skid.In with liquid and injection process, due to the temperature difference, pressure difference, stream
The factors such as speed, shearing cause the large quantities of killed or woundeds of strain, the quantity of function strain, quality is had a greatly reduced quality.So how to protect
Hold the key that oil extraction microbial activity is live injection technology.Because microbial bacterial agent has life, it can independently carry out itself
Metabolism, energy conversion, and then complete a series of activity of special vital signs such as propagation, development, heredity and variation.It is given birth to
The life cycle is divided into growth period and rest period.In growth period, microbe oil production be by using the growth of strain, metabolism, breeding,
Death process product directly, indirectly-acting in oil reservoir, play a part of improving crude oil fluidity etc..Microorganism adaptability pole
By force, Life Base is intricate in various metabolic responses in metabolism, microbial cell, is mutually restricted each other between each reaction interval
Coordinate.Change metabolic response speed rapidly with changes in environmental conditions, and athermobiosis is its self-protection, one kind of development procreation
Effective means.At present, the growth period of oil recovery strain and rest period are controlled often through control external condition.Microbial inoculum factory generally adopts
It is set to keep preferable vital sign under relatively low energy consumption with the method for low temperature process dormancy, in most cases, microbial inoculum is from going out
Factory all takes the preserving type of athermobiosis to entering before stratum.Monitoring is injected by scene to find, microbial inoculum（Rest period microbial inoculum）
Environment can not be rapidly adapted to after into stratum, it is impossible to it is quick to play a part of improving crude oil fluidity etc., so as to reduce tar productivity,
It is especially larger in the temperature difference of microbial inoculum temperature and bottom hole temperature (BHT)（More than 10 DEG C）When, bacterium colony concentration increases slower.
In addition, the injection of microbial inoculum, using pump truck or pump skid, after opening pump, pump pressure moment reaches pressure specifications value.It is many
Well known, the pressure-bearing of life entity is that have a range of, and life entity is the process progressively adapted to pressure, and many biologies are all
The moment pressure change more than 3MPa can not be born.Meanwhile the change of pressure can also cause the change of gas content in liquid, bacterium
From aerobic environment moment to oxygen-free environment, the death rate also greatly improves for agent.Furthermore the raising of pressure causes flow velocity fast, microorganism
, it is necessary to which the shearing force born is very big when crossing pump, this is one of principal element of strain injures and deaths, and the injures and deaths of strain can reduce the bacterium
The activity of kind, so as to reduce tar productivity.
The content of the invention
The invention provides a kind of method for implanting for keeping microbial activity, the deficiency of above-mentioned prior art is overcome, its
Can effectively solve the problems, such as that the activity of the microbial bacterial agent at injection well bottom existing for existing microbial bacterial agent method for implanting is not high.
The technical scheme is that realized by following measures：A kind of method for implanting for keeping microbial activity,
Carry out as follows：The first step, microbial inoculum mixed liquor is obtained after indigenous microorganism agent is mixed with microbial nutrient solution,
In microbial inoculum mixed liquor, the weight/mass percentage composition of indigenous microorganism agent is 1.5% to 2%；Second step, microbial inoculum mixed liquor is carried out
Insulation, makes the indigenous microorganism agent in microbial inoculum mixed liquor be activated, and holding temperature is less than 3 DEG C of bottom hole temperature (BHT) to shaft bottom temperature
Degree, soaking time are 1 day to 3 days；3rd step, water is pumped using pump, when the pressure of pump rises to well head pressure, by the import of pump
End is switched to from water in microbial inoculum mixed liquor, and then, pump injects into well the microbial inoculum mixed liquor after insulation, starts to be incubated in pump
During microbial inoculum mixed liquor afterwards injects into well, the rate of rise of pump pressure is controlled, uniformly boosting, the rate of rise of pump pressure are
0.8MPa/5 minutes to 1MPa/5 minutes, until pump pressure is risen to more than well head pressure 0.1MPa to 1MPa, the note of microbial inoculum mixed liquor
Inbound traffics are 0.1 cubic meters per minute to 0.5 cubic meters per minute, when pump pressure is more than well head pressure 0.1MPa to 1MPa and note
After inbound traffics are constant, continue the microbial inoculum mixed liquor into well after injection insulation until the microbial inoculum mixed liquor injection after insulation finishes.
Here is the further optimization and/or improvements to foregoing invention technical scheme：
Mentioned microorganism nutrient solution includes molasses, ammonium chloride, sodium nitrate, sodium tripolyphosphate and water, in microbial nutrient solution
In, the weight/mass percentage composition of molasses is 1.4% to 1.8%, and the weight/mass percentage composition of ammonium chloride is 0.3% to 0.5%, the matter of sodium nitrate
It is 0.7% to 0.8% to measure percentage composition, and the weight/mass percentage composition of sodium tripolyphosphate is 0.12% to 0.16%, and surplus is water.
Before the above-mentioned microbial inoculum mixed liquor into well after injection insulation, pressure testing is carried out to injection pipeline, the pressure testing time is many
In 10 minutes, pressure drop was 0MPa to 0.7MPa.
Above-mentioned make-up station obtains microbial inoculum mixed liquor after indigenous microorganism agent is mixed with microbial nutrient solution, when with
Liquid station and well site be not in Same Site, by microbial inoculum mixed liquor roping to well site by way of heat insulation transportation.
It is above-mentioned when low more than 5 DEG C than bottom hole temperature (BHT) of environment temperature, by the mistake of microbial inoculum mixed liquor heat insulation transportation to well site
Cheng Zhong, heat insulation transportation is carried out to microbial inoculum mixed liquor using boiler loaded on truck.
The method for implanting of the present invention for keeping microbial activity crosses pump in application process in indigenous microorganism agent
When, the shearing force that indigenous microorganism agent is subject to substantially reduces, and it can adapt to the change of pump pressure, so as to reduce origin
The casualty rate of microbial bacterial agent；Meanwhile before microbial inoculum mixed liquor injects into well, due to the holding temperature of indigenous microorganism agent
It is suitable with bottom hole temperature (BHT), and indigenous microorganism agent has been activated, therefore, according to holding microbial activity of the present invention
The indigenous microorganism agent that injects into well of method for implanting can rapidly adapt to the environment in shaft bottom, the indigenous microorganism can be kept
The activity of microbial inoculum, play a part of quickly improving crude oil fluidity, so as to improve the tar productivity of crude oil.
Embodiment
The present invention is not limited by following embodiments, can technique according to the invention scheme and actual conditions it is specific to determine
Embodiment.
With reference to embodiment, the invention will be further described：
Embodiment 1：The method for implanting of the holding microbial activity, carry out as follows：The first step, by indigenous microorganism
Microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, in microbial inoculum mixed liquor, the matter of indigenous microorganism agent
It is 1.5% to 2% to measure percentage composition；Second step, microbial inoculum mixed liquor is incubated, makes the indigenous microorganism bacterium in microbial inoculum mixed liquor
Agent is activated, and holding temperature is less than 3 DEG C of bottom hole temperature (BHT) to bottom hole temperature (BHT), and soaking time is 1 day to 3 days；3rd step, using pump
Water is pumped, when the pressure of pump rises to well head pressure, the entrance point of pump is switched to from water in microbial inoculum mixed liquor, then, pump
Microbial inoculum mixed liquor after insulation is injected into well, during pump starts to inject into well the microbial inoculum mixed liquor after insulation, control
The rate of rise of pump pressure is made, uniformly boosting, the rate of rise of pump pressure is 0.8MPa/5 minutes to 1MPa/5 minutes, until pump pressure rises
To being more than well head pressure 0.1MPa to 1MPa, the injection flow of microbial inoculum mixed liquor is 0.1 cubic meters per minute to 0.5 cube m/min
Clock, after pump pressure is more than well head pressure 0.1MPa to 1MPa and constant injection flow, continue into well after injection insulation
Microbial inoculum mixed liquor is until the microbial inoculum mixed liquor injection after insulation finishes.Microbial nutrient solution is common commercially available microbial nutrition
Liquid.In the method for implanting of the holding microbial activity described in the present embodiment, the microbial inoculum mixed liquor after insulation is injected into well
During, when opening pump, the pump first pumps water, the pressure of pump is risen to well head pressure, and well head pressure is risen in the pressure of pump
During, pump pressure moment increase, shear action also accordingly increases, due to pumping water rather than microbial inoculum mixed liquor in this stage, pump,
Therefore, the indigenous microorganism agent in microbial inoculum mixed liquor is not by the shear action in this stage.When the pressure of pump rises to well head pressure
Afterwards, by controlling the rate of rise and injection flow of pump pressure, when the indigenous microorganism agent is crossed pump, indigenous microorganism agent by
Shearing force substantially reduce, and it can adapt to the change of the pump pressure, so as to reduce the casualty rate of indigenous microorganism agent,
Meanwhile before microbial inoculum mixed liquor injects into well, because the holding temperature of indigenous microorganism agent is suitable with bottom hole temperature (BHT), and
Indigenous microorganism agent has been activated, therefore, the method for implanting injection well of the holding microbial activity according to the present embodiment
Interior indigenous microorganism agent can rapidly adapt to the environment in shaft bottom, can keep the activity of the indigenous microorganism agent, play
The quick effect for improving crude oil fluidity, so as to improve the tar productivity of crude oil.Microbial nutrient solution is existing known technology.I.e.
Microbial nutrient solution be those skilled in the art according to the characteristics of indigenous microorganism agent according to conventional microbiological compounding
The microbial nutrient solution that method is prepared, or, microbial nutrient solution is the public known microbial nutrient solution in this area.
Embodiment 2：The method for implanting of the holding microbial activity, carry out as follows：The first step, by indigenous microorganism
Microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, in microbial inoculum mixed liquor, the matter of indigenous microorganism agent
It is 1.5% or 2% to measure percentage composition；Second step, microbial inoculum mixed liquor is incubated, makes the indigenous microorganism bacterium in microbial inoculum mixed liquor
Agent is activated, and holding temperature is less than 3 DEG C of bottom hole temperature (BHT) or bottom hole temperature (BHT), and soaking time is 1 day or 3 days；3rd step, using pump
Water is pumped, when the pressure of pump rises to well head pressure, the entrance point of pump is switched to from water in microbial inoculum mixed liquor, then, pump
Microbial inoculum mixed liquor after insulation is injected into well, during pump starts to inject into well the microbial inoculum mixed liquor after insulation, control
The rate of rise of pump pressure is made, uniformly boosting, the rate of rise of pump pressure is 0.8MPa/5 minutes or 1MPa/5 minutes, until pump pressure rises
To well head pressure 0.1MPa or 1MPa is more than, the injection flow of microbial inoculum mixed liquor is 0.1 cubic meters per minute or 0.5 cube m/min
Clock, after pump pressure is more than well head pressure 0.1MPa or 1MPa and constant injection flow, continue into well after injection insulation
Microbial inoculum mixed liquor is until the microbial inoculum mixed liquor injection after insulation finishes.Microbial nutrient solution is existing known technology.That is microorganism
Nutrient solution is that those skilled in the art match somebody with somebody according to the characteristics of indigenous microorganism agent according to conventional microbiological compounding method
The microbial nutrient solution of system, or, microbial nutrient solution is the public known microbial nutrient solution in this area.
Embodiment 3：The method for implanting of the holding microbial activity, carry out as follows：The first step, by indigenous microorganism
Microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, in microbial inoculum mixed liquor, the matter of indigenous microorganism agent
It is 1.5% to measure percentage composition；Second step, microbial inoculum mixed liquor is incubated, makes the indigenous microorganism agent quilt in microbial inoculum mixed liquor
Activation, holding temperature are less than 3 DEG C of bottom hole temperature (BHT), and soaking time is 1 day；3rd step, water is pumped using pump, when the pressure liter of pump
During to well head pressure, the entrance point of pump is switched to from water in microbial inoculum mixed liquor, then, pump is by the microbial inoculum mixed liquor after insulation
Inject into well, during pump starts to inject into well the microbial inoculum mixed liquor after insulation, control the rate of rise of pump pressure, uniformly
Boosting, the rate of rise of pump pressure is 1MPa/5 minutes, until pump pressure is risen to more than well head pressure 1MPa, the injection of microbial inoculum mixed liquor
Flow is 0.5 cubic meters per minute, after pump pressure is more than well head pressure 1MPa and constant injection flow, continues to note into well
Enter the microbial inoculum mixed liquor after insulation until the microbial inoculum mixed liquor injection after insulation finishes.Microbial nutrient solution is existing known skill
Art.I.e. microbial nutrient solution be those skilled in the art according to the characteristics of indigenous microorganism agent according to conventional microbiological nutrient solution
The microbial nutrient solution that compound method is prepared, or, microbial nutrient solution is the public known microbial nutrient solution in this area.
Embodiment 4：The method for implanting of the holding microbial activity, carry out as follows：The first step, by indigenous microorganism
Microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, in microbial inoculum mixed liquor, the matter of indigenous microorganism agent
It is 2% to measure percentage composition；Second step, microbial inoculum mixed liquor is incubated, is swashed the indigenous microorganism agent in microbial inoculum mixed liquor
Living, holding temperature is bottom hole temperature (BHT), and soaking time is 3 days；3rd step, water is pumped using pump, when the pressure of pump rises to well head pressure
During power, the entrance point of pump is switched to from water in microbial inoculum mixed liquor, then, pump is by the microbial inoculum mixed liquor injection well after insulation
It is interior, during pump starts to inject into well the microbial inoculum mixed liquor after insulation, the rate of rise of pump pressure is controlled, uniformly boosting,
The rate of rise of pump pressure is 0.8MPa/5 minutes, until pump pressure is risen to more than well head pressure 0.1MPa, the injection of microbial inoculum mixed liquor
Flow is 0.1 cubic meters per minute, after pump pressure is more than well head pressure 0.1MPa and constant injection flow, is continued into well
Microbial inoculum mixed liquor after injection insulation is until the microbial inoculum mixed liquor injection after insulation finishes.Microbial nutrient solution is existing known skill
Art.I.e. microbial nutrient solution be those skilled in the art according to the characteristics of indigenous microorganism agent according to conventional microbiological nutrient solution
The microbial nutrient solution that compound method is prepared, or, microbial nutrient solution is the public known microbial nutrient solution in this area.
Embodiment 5：As the optimization of above-described embodiment, microbial nutrient solution includes molasses, ammonium chloride, sodium nitrate, trimerization
Sodium phosphate and water, in microbial nutrient solution, the weight/mass percentage composition of molasses is 1.4% to 1.8%, and the quality percentage of ammonium chloride contains
Measure as 0.3% to 0.5%, the weight/mass percentage composition of sodium nitrate is 0.7% to 0.8%, and the weight/mass percentage composition of sodium tripolyphosphate is
0.12% to 0.16%, surplus is water.
Embodiment 6：As the optimization of above-described embodiment, before the microbial inoculum mixed liquor into well after injection insulation, to injection
Pipeline carries out pressure testing, and the pressure testing time is no less than 10 minutes, and pressure drop is 0MPa to 0.7MPa.Pressure testing operation is able to ensure that injection operation
Safe and smooth progress.
Embodiment 7：As the optimization of above-described embodiment, indigenous microorganism agent and microbial nutrient solution are entered in make-up station
Microbial inoculum mixed liquor is obtained after row mixing, when make-up station and well site be not in Same Site, by microbial inoculum by way of heat insulation transportation
Mixed liquor roping is to well site.Because indigenous microorganism agent is the local strain in area that recovers the oil, therefore apart from close, therefore when transporting
Between within one day, the time of heat insulation transportation is transporting indigenous microorganism agent in the soaking time described in above-described embodiment
It can also be activated during defeated, be easy to microbial inoculum mixed liquor quickly to carry out injection operation after transporting to well site.
Embodiment 8：As the optimization of above-described embodiment, when low more than 5 DEG C than bottom hole temperature (BHT) of environment temperature, by microbial inoculum
During mixed liquor heat insulation transportation to well site, heat insulation transportation is carried out to microbial inoculum mixed liquor using boiler loaded on truck.When environment temperature
When spending more than 5 DEG C lower than bottom hole temperature (BHT), when carrying out heat insulation transportation to microbial inoculum mixed liquor using boiler loaded on truck, the micro- life of origin can be made
Thing microbial inoculum can also be activated in transportation, be easy to microbial inoculum mixed liquor quickly to carry out injection work after transporting to well site
Industry.
The method for implanting of holding microbial activity described in embodiment 1, embodiment 3, embodiment 4 and embodiment 5 is carried out
Using the reservoir condition of application：（1）Temperature：Reservoir temperature is with 30 DEG C to 50 DEG C；（2）Pressure：10.5MPa to 21.0MPa；（3）
Salinity：Total salinity is less than 10000 mg/L；（4）Oil density:The density of crude oil is less than 0.9659 g/cm3；（5）PH value:
It is neutral；（6）Permeability and porosity:Permeability is not less than 0.05 μm2, porosity is more than 15%；（7）Saturation degree:Stratum remaining oil
Saturation degree is more than 25%；（8）Content of beary metal:Contents of heavy metal elements in the water of stratum is less than 10mg/L.Measure is applied in the oil
It is dense with the bacterium of indigenous microorganism agent of the injection well bottom after 1 day before the indigenous microorganism agent injection well bottom of layer condition（Quality hundred
Divide ratio）, the bacterium of indigenous microorganism agent behind injection well bottom 1 day is dense to be not less than the indigenous microorganism agent before injection well bottom
Bacterium it is dense 0.7（Level of activity）When, you can illustrate that the activity of the indigenous microorganism agent in shaft bottom is effectively kept.Using reality
Apply the indigenous microorganism agent of the method for implanting of the holding microbial activity described in example 1, embodiment 3, embodiment 4 and embodiment 5
Before injection well bottom（Before injection）With behind injection well bottom 1 day（After injection）Bacterium it is dense（%）As shown in table 1.Using existing injection side
Method is when the oil reservoir is applied, using existing method for implanting（Existing method）Indigenous microorganism agent before injection well bottom（Injection
Before）With behind injection well bottom 1 day（After injection）Bacterium it is dense（%）As shown in table 1.Using embodiment 1, embodiment 3, embodiment 4 and reality
Apply the level of activity of the method for implanting of the holding microbial activity described in example 5 and the indigenous microorganism agent of existing method for implanting
As shown in table 1, dense/preflood bacterium of bacterium after level of activity=injection is dense.
It can be seen from Table 1 that the holding microorganism according to embodiment 1, embodiment 3, embodiment 4 and embodiment 5
The level of activity of the indigenous microorganism agent of the method for implanting injection of activity is all remarkably higher than using the injection of existing method for implanting
The level of activity of indigenous microorganism agent, illustrate that the holding according to embodiment 1, embodiment 3, embodiment 4 and embodiment 5 is micro-
The method for implanting of bioactivity can improve the survival rate of indigenous microorganism agent, so as to keep indigenous microorganism agent to exist
The activity in shaft bottom.
In addition, the injection side of the holding microbial activity according to embodiment 1, embodiment 3, embodiment 4 and embodiment 5
Method injected indigenous microorganism agent after 24 days, and hydrocarbon oxidizing bacteria, the zymogenic order of magnitude in the indigenous microorganism agent of shaft bottom carry
High 1 to 5 order of magnitude, the holding microorganism according to embodiment 1, embodiment 3, embodiment 4 and embodiment 5 is absolutely proved
The method for implanting of activity can keep indigenous microorganism agent in the activity in shaft bottom, so as to be advantageous to the raising of tar productivity.
In summary, the method for implanting of the present invention for keeping microbial activity is in application process, in the micro- life of origin
When thing microbial inoculum crosses pump, the shearing force that indigenous microorganism agent is subject to substantially reduces, and it can adapt to the change of pump pressure, so as to
Reduce the casualty rate of indigenous microorganism agent；Meanwhile before microbial inoculum mixed liquor injects into well, due to indigenous microorganism agent
Holding temperature it is suitable with bottom hole temperature (BHT), and indigenous microorganism agent has been activated, therefore, according to holding of the present invention
The indigenous microorganism agent that the method for implanting of microbial activity injects into well can rapidly adapt to the environment in shaft bottom, can keep this
The activity of indigenous microorganism agent, play a part of quickly improving crude oil fluidity, so as to improve the tar productivity of crude oil.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can basis
The non-essential technical characteristic of increase and decrease is actually needed, to meet the needs of different situations.
Table 1
| Before injection（%） | After injection（%） | Level of activity | |
| Embodiment 1 | 1.5 to 2 | 1.215 to 1.84 | 0.81 to 0.92 |
| Embodiment 3 | 1.5 | 1.215 | 0.81 |
| Embodiment 4 | 2 | 1.84 | 0.92 |
| Embodiment 5 | 1.7 | 1.428 | 0.84 |
| Existing method | 2 | 0.86 | 0.43 |
Claims (7)
1. a kind of method for implanting for keeping microbial activity, it is characterised in that carry out as follows：The first step, by the micro- life of origin
Thing microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, in microbial inoculum mixed liquor, indigenous microorganism agent
Weight/mass percentage composition is 1.5% to 2%；Second step, microbial inoculum mixed liquor is incubated, makes the indigenous microorganism in microbial inoculum mixed liquor
Microbial inoculum is activated, and holding temperature is less than 3 DEG C of bottom hole temperature (BHT) to bottom hole temperature (BHT), and soaking time is 1 day to 3 days；3rd step, use
Pump pumps water, and when the pressure of pump rises to well head pressure, the entrance point of pump is switched to from water in microbial inoculum mixed liquor, then,
Pump injects into well the microbial inoculum mixed liquor after insulation, during pump starts to inject into well the microbial inoculum mixed liquor after insulation,
The rate of rise of pump pressure is controlled, uniformly boosting, the rate of rise of pump pressure is 0.8MPa/5 minutes to 1MPa/5 minutes, until pump pressure
Rise to and be more than well head pressure 0.1MPa to 1MPa, the injection flow of microbial inoculum mixed liquor for 0.1 cubic meters per minute to 0.5 cubic metre/
Minute, after pump pressure is more than well head pressure 0.1MPa to 1MPa and constant injection flow, continue into well after injection insulation
Microbial inoculum mixed liquor until insulation after microbial inoculum mixed liquor injection finish.
2. the method for implanting according to claim 1 for keeping microbial activity, it is characterised in that microbial nutrient solution includes
Molasses, ammonium chloride, sodium nitrate, sodium tripolyphosphate and water, in microbial nutrient solution, the weight/mass percentage compositions of molasses for 1.4% to
1.8%, the weight/mass percentage composition of ammonium chloride is 0.3% to 0.5%, and the weight/mass percentage composition of sodium nitrate is 0.7% to 0.8%, trimerization phosphorus
The weight/mass percentage composition of sour sodium is 0.12% to 0.16%, and surplus is water.
3. the method for implanting according to claim 1 or 2 for keeping microbial activity, it is characterised in that insulation is injected into well
Before microbial inoculum mixed liquor afterwards, to injection pipeline carry out pressure testing, the pressure testing time be no less than 10 minutes, pressure drop be 0MPa extremely
0.7MPa。
4. the method for implanting according to claim 1 or 2 for keeping microbial activity, it is characterised in that make-up station is micro- by origin
Bacteria agent obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, when make-up station and well site be not in Same Site,
By microbial inoculum mixed liquor roping to well site by way of heat insulation transportation.
5. the method for implanting according to claim 3 for keeping microbial activity, it is characterised in that make-up station is by the micro- life of origin
Thing microbial inoculum obtains microbial inoculum mixed liquor after being mixed with microbial nutrient solution, when make-up station and well site be not in Same Site, lead to
The mode of heat insulation transportation is crossed by microbial inoculum mixed liquor roping to well site.
6. the method for implanting according to claim 4 for keeping microbial activity, it is characterised in that when environment temperature compares shaft bottom
When temperature is low more than 5 DEG C, during by microbial inoculum mixed liquor heat insulation transportation to well site, using boiler loaded on truck to microbial inoculum mixed liquor
Carry out heat insulation transportation.
7. the method for implanting according to claim 5 for keeping microbial activity, it is characterised in that when environment temperature compares shaft bottom
When temperature is low more than 5 DEG C, during by microbial inoculum mixed liquor heat insulation transportation to well site, using boiler loaded on truck to microbial inoculum mixed liquor
Carry out heat insulation transportation.
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