CN103912254B - A kind of method utilizing compounding activation agent to improve fracturing well capacity - Google Patents
A kind of method utilizing compounding activation agent to improve fracturing well capacity Download PDFInfo
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Abstract
The present invention is a kind of method utilizing compounding activation agent to improve fracturing well capacity.It is will to include the compounding activation agent A of electron acceptor and nutrient or also include that the compounding activation agent B of microbial bacterial agent introduces formation at target locations, controlled propagation and the metabolic activity of microorganism by selectivity in formation at target locations, remove stratum pollutant and generate beneficial metabolic product, the method improving fractured well production capacity.The present invention produces microbiological field in the reservoir space of formation at target locations, remove the guanidine glue filter cake remained because of pressure break or the heavy hydrocarbon contaminants caused because of production of hydrocarbons, improve the flow conductivity of hydraulic fracture body, generate useful microbial metabolic products simultaneously, reduce profit boundary tension force, emulsified crude oil also improves its mobility, thus improves pressing crack construction effect of increasing production, improves fractured well production capacity.
Description
Technical field
The present invention relates to a kind of biological fracturing fluid in oil drilling fracturing fluid field, say further, relate to
A kind of method utilizing compounding activation agent to improve fracturing well capacity.
Background technology
Pressure break as the Main Yield-increasing means of the important means of oil-gas field development, particularly Low permeable oil and gas reservoirs,
While construction, also it is formed about injury band at crack wall, causes the filtrational resistance of substrate to increase, impact
The raising of production capacity.In practical operation, more breaking glue solution after fracturing liquid rubber-breaking, is had to be trapped in stratum, no
Can completely return and be discharged to ground.Owing to breaking glue solution containing various chemical compositions, itself and formation rock mineral effect
Producing multiple effect, the vector superposed of several effects can affect Reservoir Seepage ability, is usually expressed as negative shadow
Ring.It is mainly manifested in following aspect: 1. pressure break residue and filter cake, a large amount of water-insolubles in aqueous fracturing fluid
Stratum particularly leak-off layer can be caused serious injury by existence；2. the change of capillary force.Fracturing liquid rubber-breaking liquid
Invaded formation must change wetting contact angle and interfacial tension, thus causes the change of capillary force；3. clay is swollen
Swollen and particle migration.Fracturing fluid mostly is aqueous fracturing fluid at present, after invading stratum, anti-with clay mineral matter
Should, there is aquation, expansion, make pore throat radius reduce, or particle migration occurs, blocking pore throat forms bridge blinding,
Directly affect fluid flowing；4. water lock and gas lock effect.When pressure break breaking glue solution enters stratum, easily mix with oil
Produce water-blocking effect or mix generation gas lock effect blocking capillary channel with gas, having a strong impact on the seepage flow energy of reservoir
Power.
On the other hand, fracturing yield increasing prolongation in time, effect of increasing production is gradually lowered.Different oil wells due to
Specific construction and the difference of formation condition, effect duration is the most otherwise varied.The linear stream of post-fracturing production capacity, plan
Radial flow and radial flow three phases.At the end of pseudoradial flow, should be regarded as pressure break invalid, the most often adopt
With refracturing, though oil well output can be improved again, but energy consumption and input are huge.Find through analyzing,
Fracturing effect is often relevant to the flow conductivity of crack body, and in oil well production process, in crude oil, heavy component is past
Toward depositing, shaking out and formation water of stratum due near wellbore zone pressure and the change of flow velocity, chromatograph effect etc.
Fouling can cause the injury of crack body permeability equally, ultimately causes the disappearance of fracturing effect.Visible, for
Property the formation damage released in crack body be to ensure pressing crack construction effect, reduce operating cost, improve economy
The important means of benefit.
Microbial Enhanced Oil Recovery has small investment, and economic and environment-friendly, the feature of effect duration length, its mechanism is general
Think from two broad aspect: i.e. the effect of microbial cells itself and the effect of microbial metabolic products, the former
Mainly include the physical action of microbial cells and the microorganism Degradation for crude oil He other pollutant,
The latter mainly comprises the various metabolites of microorganism, as Bio-surface active material, small molecular organic acid,
Gas, organic solvent, biopolymer etc..The effective object of microbial metabolism and metabolite thereof can be
Formation fluid (formation water, crude oil or gas), it is also possible to be pore media (rock).Therefore, by micro-
In biological introducing Fracture System, by the pollutant in its propagation and metabolic activity degraded crack body, give birth to simultaneously
Become beneficial metabolic product, it is possible to continuous action also alleviates crack body permeability injury, and can be by reducing oil
Aqueous phase interface tension force, the reduction viscosity of crude such as emulsifying and Degradation, raising oil recovery factor, thus significantly
Improve pressing crack construction effect of increasing production.
Application publication number is that the Chinese patent (Application No. 201010215947.6) of CN101880523A carries
Gone out a kind of to utilize enzyme-microbe coupled technology realizing fracturing liquid rubber-breaking, fracturing process by microorganism and
The broken glue effect of enzyme synergy strengthening.By efficient gel breaking enzyme and microbe coupled process fracturing fluid, it is achieved more
High broken glue efficiency, reduces pressure break residue.Before the prior art mainly acts on fracturing fluid recovery (backflow), in construction
The broken glue time relatively short, microorganism is had an effect limited, although can to a certain degree alleviate fracturing stratum
Injury, but cannot realize improving for a long time the purpose of crack body flow conductivity, with less improving oil recovery factor
Function.
Application publication number is that the Chinese patent (Application No. 200910197996.9) of CN101699026A carries
Go out a kind of method carrying out Microbial Enhanced Oil Recovery in LOW PERMEABILITY RESERVOIR.This technology mainly utilizes the oil-field flooding displacement of reservoir oil
Technique, adds microorganism fungus kind in injecting water, utilizes its characteristic metabolic products such as acid, gas, biological table
Face activating agent, solvent etc. improve the development effectiveness of low-permeability oil deposit.Owing to LOW PERMEABILITY RESERVOIR permeability is relatively low, often
Difficulty is injected in rule microorganism, it is impossible to realize effective migration, is more easy to be formed thalline filter cake near wellbore zone, should
Although proposing in technology can be at fractured well microbe oil recovery technique, but and unresolved above-mentioned technical barrier.
Summary of the invention
For solving the problem that prior art exists, the one that the present invention provides utilizes compounding activation agent to improve waterpower
The method of fractured well production capacity is to control the propagation of microorganism and metabolic activity by selectivity and realize, specifically
It is to realize by the way of changing and introducing microbial bacterial agent, nutrient and electron acceptor.The present invention utilizes
Compounding activation agent activating microorganisms in formation at target locations of the present invention, degrades ground by the metabolism of microorganism
Layer pollutant, generate beneficial metabolic product, it is achieved solve plugging function, improve the flow conductivity of crack body, improve
Oil recovery factor, improves fractured well production capacity.
A kind of method utilizing compounding activation agent to improve fracturing well capacity of the present invention is achieved in that
Described method is that compounding activation agent A is introduced the fracturing fracture of formation at target locations and neighbouring subterranean formation zone,
By propagation and the metabolic activity of activating microorganisms in formation at target locations, remove stratum pollutant and generate useful generation
Thank to product, the method improving fractured well production capacity；Wherein,
Described compounding activation agent A includes the nutrient solution that weight concentration is 1.0～10wt% of nutrient;
Described nutrient is made up of carbon source, nitrogen source, inorganic salt, liquid microelement, somatomedin；Wherein, weight
Amount proportion of composing is carbon source 50～75wt%, nitrogen source 10～20wt%, inorganic salt 10～20wt%, trace unit
Element liquid 2.5～5.0wt%, growth factor-2 .5～5.0wt%.
Described carbon source is selected from least one in saccharide, alcohols, acids, alkanes；Described nitrogen source can
At least one in peptone, Carnis Bovis seu Bubali cream, ammonium salt, nitrate, nitrite, amine；Described
Inorganic salt, is alternatively used for the phosphorus needed for supplementary growth of microorganism, sulfur, potassium, sodium, calcium, magnesium, ferrum element；
Described liquid microelement, comprise for can supplement cobalt needed for growth of microorganism, zinc, molybdenum, nickel, tungsten,
The elements such as copper；Described somatomedin be selected from yeast powder, vitamin B1, vitamin B2, vitamin B6,
Vitamin B12, nicotiamide, pantothenic acid, folic acid, biotin, thioctic acid, inositol, choline, haemachrome.Institute
The carbon source stated, nitrogen source, inorganic salt, liquid microelement, the concrete component of somatomedin all select this area normal
Component.
When carrying out anaerobic fermentation, the compounding activation agent A only comprising nutrient can be injected formation at target locations.
Described compounding activation agent A may also include electron acceptor.
Described electron acceptor one in aerobic electron acceptor, denitrification electron acceptor；
At least one in air, oxygen of described aerobic electron acceptor；Described denitrification electronics is subject to
At least one in nitrate, nitrite of body；
Wherein,
Preparation compounding activation agent A concretely comprises the following steps:
When compounding activation agent A only comprises nutrient solution, described compounding activation agent A for water by institute
State nutrient and be diluted to the aqueous solution of 1.0～10wt% concentration；
When in compounding activation agent A in addition to nutrient solution possibly together with electron acceptor time, be divided into two kinds of situations:
1) when electron acceptor positro receptor preferably, in concrete enforcement, described nutrient solution is become reconciled
Positro receptor volume ratio diversity is very big, can be the mixture of 1:3～1:40, preferably 1:3～1:20,
More preferably 1:3～1:10, more more preferably 1:3～1:6；Wherein nutrient solution is by nutrient with water
It is diluted to the solution of 1.0～10wt% concentration.In being embodied as, can be mixed under formation condition high pressure, can adopt
The mode taking ground air compressor pump air interval mixed water injection injects stratum；
2) when electron acceptor is denitrification electron acceptor, described compounding activation agent A be containing 1.0～
10wt% nutrient and the aqueous solution of 1.0～5.0wt% electron acceptors；Electron acceptor is in compounding activation agent A
Weight concentration be preferably 1.5～3wt%, more preferably 2wt%.
Described compounding activation agent A also can add microbial bacterial agent, with the addition of the compounding activation agent of microbial bacterial agent
A constitutes compounding activation agent B；
In described compounding activation agent B containing with volume basis be the compounding activation total liquid phase volume of agent B 0.1～
The microbial bacterial agent of 5.0%, preferably 1.0～the microbial bacterial agent of 5.0%；
Wherein, when electron acceptor positro receptor preferably, the described compounding activation total liquid phase volume of agent B is
Nutrient solution and the cumulative volume of microbial bacterial agent；
When electron acceptor is denitrification electron acceptor, the described compounding activation total liquid phase volume of agent B is compound
Activator A and the cumulative volume of microbial bacterial agent；
Described microbial bacterial agent is containing 1 × 107～1 × 1010The culture fluid of CFU/ml strain concentration, is preferably
1×107～1 × 109The culture fluid of CFU/ml strain concentration.
The source of described microorganism fungus kind can be isolated original inhabitants bacterium from construction well mud, it is also possible to be
Introduce the external source strain in construction well；
Described microbial bacterial agent is containing at least one microorganism in aerobic, amphimicrobian or anaerobe
The microbial culture medium of strain, described microorganism fungus kind can be guanidine glue degrading microorganism, heavy hydrocarbon degraded micro-
Biological or metabolite is conducive at least one in the microorganism of scale removal matter；Specifically, described micro-life
Microorganism fungus kind in thing microbial inoculum is selected from Rhodopseudomonas (Pseudomonas) microorganism, bacillus
(Bacillus) at least one in microorganism, Clostridium (Clsotridium) microorganism；
Preferably, the described microorganism fungus kind in microbial bacterial agent is selected from Pseudomonas aeruginosa
(Pseudomonas aeruginosa), bacillus subtilis (Bacillus subtilis), Clostridium butyricum
(Clsotridium butyricum) and clostridium acetobutylicum (Clsotridium acetobutyricum) are at least
A kind of；
The concrete steps preparing microbial bacterial agent can be: microorganism fungus kind is placed in Zymolysis Equipment cultivation,
At microorganism optimum growth temperature bottom fermentation 12～120h, it is thus achieved that 1 × 107～1 × 1010Dense the sending out of CFU/ml bacterium
Ferment liquid is as microbial bacterial agent；
This method includes two kinds of embodiments:
A, directly activates original indigenous microorganism in oil reservoir, can be by whether having foot in early stage detection stratum
Reach concentration and possess the microorganism of correlation function, in this way, putting into only nutrient or nutritious thing and electronics and be subject to
The compounding activation agent A of body composition；
B, if lacking enough concentration in stratum and possessing the microorganism of correlation function, the most preferably uses by nutrient
Compounding activation that is that form with microbial bacterial agent or that collectively constituted by nutrient, electron acceptor and microbial bacterial agent
Agent B.
The present invention be through regulating the above-mentioned kind of electron acceptor, nutrient and microbial bacterial agent, quantity,
Concentration, injection mode, injection cycle realize.
As, in concrete enforcement, it is less than 2 × 10 when oil well produced liquid bacterium is dense5During CFU/ml, need to be by compound
Activator B carries out external source and supplements strain；After supplementary strain, if the strain supplemented is survived well in the earth formation,
The strain concentration that Produced Liquid detects is higher than 2 × 105During CFU/ml, then in the process of subsequent cycle, can be only
Inject compounding activation agent A, without being supplemented with external source strain.
Described method comprises the steps:
After pressing crack construction, it was a cycle to inject once-combined activator A or compounding activation with every 5～10 days
Agent B, 1～2 times that compounding activation agent volume is artificial crevice volume every time injected, carry out 3～5 altogether
The individual cycle.
And,
In normal productive process, when oil well liquid-producing index is less than normal production value more than 30%, can be according to
Within every 3～7 days, being a cycle to inject once-combined activator A or compounding activation agent B, that injects is compound every time
Volume is artificial crevice volume 1～2 times of activator A or compounding activation agent B, carries out 1～3 altogether
Cycle.
The volume value of described man-made fracture is calculating estimation value general in this area, and described is artificial
Crevice volume computational methods are: V=2*L*a*h, and wherein L is that dummy joint is long, and a is seam width, and h is seam height；After
Continuous bolus injection calculation method of physical volume is ibid.
Described formation contaminant thing, including fracturing fluid residue, the waxiness of crude oil heavy component, colloid or drip
At least one in blue or green matter and formation water fouling；
Described beneficial metabolic product includes that gas, biosurfactant, small molecule solvent, little molecule have
At least one in machine acid；
In described beneficial metabolic product,
Described gas, including CO2、H2、CH4、N2In at least one；
Described biosurfactant, including glycolipid class, lipopeptid class, lipoprotein, sugar-protein-lipid complex
At least one of apoplexy due to endogenous wind；
Described small molecule solvent, including ethanol, propanol, isopropanol, butanol, butanediol, isoamyl alcohol,
At least one in acetone；
Described small molecular organic acid, including in formic acid, acetic acid, propanoic acid, butanoic acid, lactic acid, oxalic acid extremely
Few one.
In sum, the present invention controls propagation and the metabolic activity of microorganism by selectivity, it is possible to make micro-life
Thing can remove stratum pollutant in formation at target locations effectively, thus improves fisstured flow system flow conductivity.
Described formation at target locations has following condition: have suitable physics, chemistry and biological factor, including temperature
Degree, pressure, osmotic pressure, porosity, permeability, formation water salinity, pH value, heavy metal, Tu Zhuwei
Biome, nutritional substrate and electron acceptor etc..
Specifically, when implementing the method for the present invention, should select to meet the oil reservoir conduct of growth of microorganism demand
Target reservoir.From angle biology, stratum can be regarded as a huge fermentable container, ground
The environmental condition of layer plays the strictest effect to the activity of microorganism, and extreme influence the growth of microorganism
And metabolic activity, in stratum, the principal element of restriction micro-organisms growth and metabolism can be summarized as following side
Face:
(1) physical factor: temperature, pressure, osmotic pressure, porosity and permeability etc.；
(2) chemical factor: formation water salinity, pH value, heavy metal etc.；
(3) biological factor: oil reservoir indigenous microorganism group, nutritional substrate and electron acceptor etc..
The all respective oil reservoir to Microbial Enhanced Oil Recovery of Russia, the U.S. and China selects to have formulated some standards
(table 1).All in all, the foundation that in these standards, parameter is arranged is mainly for growth and the metabolism of microorganism
One relatively mild reservoir media is provided, higher active and relatively active to ensure that oil extraction microbial has
Metabolism behavior, can formation at target locations described in assisting sifting this method by these standards.
Table 1 reservoir condition screening criteria
The present invention fully combines the feature of Microbial Enhanced Oil Recovery, it is combined with hydraulic fracturing technology, energy
Enough it is effectively improved the effect of increasing production of fracturing.By the selective indigenous microorganism or excellent activated in stratum
The inoculating microbe of choosing, it is achieved continue the purpose of de-plugging: after pressure break terminates, main formation contaminant thing is
Water-based fracturing residue, guanidine glue degrading microorganism of stress living is main；Along with production of hydrocarbons, crude oil heavy component is hindered
Evil increases the weight of, should be to activate heavy hydrocarbon degrading microorganism.Regulated and controled by manual selectivity, real in the body of crack
The microbiological field of existing certain rule, persistence improves the flow conductivity of crack body, meanwhile, micro-life of activation
The multiple beneficial metabolite that thing generates can improve oil recovery factor further, thus ensures that fracturing is executed
The action effect of work.
The invention have the characteristics that
1) present invention can selectively add electron acceptor in compounding activation agent；
2) in application, method is more flexible, and the present invention can optionally be only injected into electron acceptor and nutrient
And do not introduce microbial bacterial agent；
3) present invention is according to the difference of practical situation, can regulate the component of compounding activation agent neatly.
Present invention solves the technical problem that and be: conventional microbiological injects difficulty, it is impossible to realize effective migration,
It is more easy to be formed near wellbore zone thalline filter cake, plays raising production capacity effect the biggest；The present invention can be preferably
Activate indigenous microorganism or activate inoculating microbe, it is achieved solving plugging function, improve the flow conductivity of crack body,
Improve oil recovery factor.
The advantage that the present invention highlights is, is controlled propagation and the metabolism of microorganism in stratum by selectivity, removes
Pollutant in the body of crack, improve the flow conductivity of hydraulic fracture osmotic system, extend pressing crack construction
Effect duration, and by the useful metabolite that microorganism generates, improve oil recovery factor, it is achieved that change
The purpose of kind water-based fracturing well capacity.
Detailed description of the invention
Below in conjunction with embodiment, further illustrate the present invention.
Embodiment 1
Fracturing fluid residue character to be measured is tested:
For disclosing the source of fracturing fluid residue, contrived experiment is as follows:
1. prepare the aqueous solution of each hydraulic fracturing additive with on-the-spot water sample, observe after standing a couple of days.
2. take single solution to mix mutually, observe compatibility each other.
The most such as separate out precipitation, the precipitation and centrifugal separation that will separate out, and measure the quality after 105 DEG C of drying constant weights.
The water-insoluble amount of separate sources in table 2HPG fracturing fluid (1L)
Note: 1.3000r/min is centrifuged 20 minutes volumes；2. Puyang is with boat ceramsite sand factory 0.45～0.90mm, 69MPa haydite.
Test result: containing a small amount of black water-insoluble in potassium chloride solution, add strong acid after leaching and be dissolved as
Pale brown liquid, adding ammonium thiocyanate is peony, is accredited as rust.Sodium hydroxide produces white casse after adding water,
Theobromine is molten, it may be possible to containing a small amount of calcium ions and magnesium ions in water.Other additive has no adverse reaction.Thus speculate,
Owing to formation water also often containing substantial amounts of high volence metal ion, may also have with alkaline fracturing fluid filtrate
Precipitation.
During solution combination, only potassium chloride and sodium hydroxide solution (clear liquid after precipitation), borate crosslinker and mistake
Ammonium sulfate gel breakers etc., produce more white precipitate after mixing, other combination has no adverse reaction.Scene is taken
The potassium chloride analysis returned shows, wherein contains the calcium of 0.35% and the magnesium of 0.032%.Mixing produces hydroxide respectively
Magnesium, Calcium pyroborate or persulfuric acid calcium etc. precipitate.
Table 2 illustrates, in fracturing fluid, water-insoluble is mainly derived from the dust that proppant carries in terms of quality, from body
Long-pending seeing is mainly derived from HPG rubber powder.Owing to HPG is the modified product of guar gum, a kind of pulse family taken from by guar gum
The endosperm of plant, common processing separates and can not be kept completely separate by other composition of seed.Water in guar gum
The dyed Qualitative Identification of insoluble matter is mainly cellulose family and protein-based.
Embodiment 2
Guanidine glue anaerobic degradation is tested:By Clostridium butyricum (Clsotridium butyricum CGMCC1.336), and third
Ketone Clostridium acetobutylicum (Clsotridium acetobutyricum CGMCC1.70) is tested for guanidine glue anaerobic degradation,
Wherein, in Clostridium butyricum microbial inoculum, Clostridium butyricum concentration is 2 ~ 5 × 107CFU/ml, this microbial bacterial agent is filtrate
In consumption with volume basis as filtrate volume 1%；In clostridium acetobutylicum agent, microorganism concn is
2~5×107CFU/ml, this microbial bacterial agent consumption in filtrate with volume basis as filtrate volume 1%；
Its broken glue in the earth formation is detected by the method for " SY/T5107-2005 water based pressure liquid method of evaluating performance "
Ability.Test is being filled with N2Lactic acid pipe in carry out, using Ammonium persulfate. (APS) guanidine glue after breaking gel as battalion
Support substrate, lactic acid pipe adds Du Shi pipe and observes gas generation situation, utilize the gas chromatogram generation to three strain bacterium
Thanking to product to detect, result is as follows:
The typical clostridial metabolite of table 3
* in culture medium, guanidine gum concentration is 18g/L.
By testing discovery above, Clostridium butyricum, acetone Clostridium butyricum can under anaerobic be degraded brokenly glue
After guanidine glue component, generate based on gas, the metabolite of solvent.
Embodiment 3
Microorganism is for guanidine glue injury removal of bridge formation henchnmrk test:Use pine south igneous rock hyposmosis natural core,
Natural core is answered reservoir fluid parallel direction of flowing drill through cylinder, grinding two end surfaces, and with smooth
The face of cylinder is perpendicular.Core diameter is 25mm～25.4mm or 37mm～38mm, and rock core is a length of directly
1～1.5 times of footpath.Prepare the rock core of three same sizes by above-mentioned standard, be respectively designated as rock core A, rock
Heart B, rock core C, remain to test standby；Rock core cleans and dries presses SY/T5336-1996 execution, cleans and dries
The gas permeability of the rock core after Gan and pore volume measure presses SY/T5336-1996 execution.Rock after mensuration
The heart is set up irreducible water saturation by SY/T5336-1996 and measures permeability K before guanidine glue injures1, make simulation
Formation water is clamp-oned rock core from core holding unit backward end and is carried out displacement, and the flow velocity of saline is less than critical flow velocity.Directly
Stable to flow and pressure reduction, stabilization time is no less than 60min.
Core permeability is calculated by following equation:
Wherein, K core permeability, μm2；The volume flow of Q flow media, cm3/s；
The viscosity of μ flow media, mPa s；L rock core axial length, cm；
The pressure reduction that Δ p rock core is imported and exported, MPa；The cross-sectional area of A rock core, cm2。
Rock core damage process (uses rock core A): the fracturing fluid filtrate prepared by SY/T5107-2005 loaded
In high-pressure bottle, pressurize with pressure source, make filtrate inject rock core A from core holding unit forward end.When filtrate is opened
When beginning to flow, record time, the accumulation filter loss of filtrate, be accurate to 0.1mL.During mensuration, during mensuration
Between be 36min.After having squeezed, close clamper two ends valve, make filtrate stop 2h in rock core.Test
Temperature is fracturing fluid Applicable temperature (≤80 DEG C), and measures the core permeability K after infringement2。
Microorganism de-plugging process (uses rock core B and rock core C) respectively: carry out rock core according to above-mentioned experimentation
Displacement experiment, except for the difference that all accesses the compounding activation agent accounting for filtrate volume 5.0% in filtrate；Described compound
Activator contains with microbial bacterial agent that volume basis is 1.0% with containing the concentration nutrient as 5.0wt%, its
In, the nutrient amounts of components ratio of compounding activation agent is shown in Table 4, and this compounding activation agent does not contains electron acceptor, to enter
Row anaerobic fermentation.
The nutrient inventory table of table 4 compounding activation agent
Wherein, the component formula of liquid microelement (A) and somatomedin liquid be shown in Table 5, table 6.
Table 5 trace element formula of liquid (A)
Table 6 somatomedin formula of liquid
Using the microbial bacterial agent used in the experiment of rock core B to contain concentration is 2 ~ 5 × 107The butanoic acid of CFU/ml
Clostridium (Clsotridium butyricum CGMCC1.336)；Use the microorganism used in the experiment of rock core C
It is 2 ~ 5 × 10 that microbial inoculum contains concentration7Clostridium acetobutylicum (the Clsotridium acetobutyricum of CFU/ml
CGMCC1.70), close clamper two ends valve after inoculation, make filtrate cultivate 72h in rock core, again survey
Determine core permeability K3, experimental result is shown in Table 7.
The core permeability of test determination is as follows:
Permeability loss rate computing formula is:
Resume permeability rate computing formula is:
Wherein, K1Rock core original permeability, μm2；
K2Permeability after rock core damage, μm2；
K3Permeability after rock core microorganism de-plugging, μm2。
Table 7 core permeability
Provable by upper table data: fracturing fluid filter cake, filtrate are for stratum substrate, particularly low permeability formation
Injury rate especially serious, local can reach 70～80%, by the Degradation of microorganism, it is possible to effectively go
Except the guanidine glue stain thing in stratum, it is achieved in-place permeability have efficient recovery.
Embodiment 4
On-the-spot test:Northeast hyposmosis viscous crude field, this block viscosity of crude is higher, and gas-oil ratio is relatively low, with opening
The prolongation of the time of sending out, the pollution of crack body all various degrees near well wellbore, raw to oil extraction in oil field
Produce and cause the biggest difficulty, for improving oil well productivity, improve block whole development effect, to wherein 16 mouthfuls of pressures
Split brought in well and carry out microorganism de-plugging operation.
The strain of the microbial bacterial agent used in on-the-spot test is isolated amphimicrobian from produced liquid in oil well
Microorganism, is Rhodopseudomonas (Pseudomonas sp.) for 16S rDNA sequence analysis, its main metabolic
Product is carboxylate and the biosurfactant of glycolipid class, and microbial bacterial agent is brownish red dense fluids, bacterium
Concentration 2.5 × 108CFU/ml。
The compounding activation agent used is compounding activation agent B, and it comprises the aqueous nutrient solution and 5 of 1 times of volume ratio
The air (aerobic electron acceptor) of times volume ratio, contains the microbial bacterial agent as inoculum simultaneously, described
Microbial bacterial agent accounts for the 2.0% of the compounding activation total liquid phase volume of agent B with volume basis.Wherein, nutrient is water-soluble
Liquid component is shown in Table 8.
Table 8 aqueous nutrient solution
Wherein, the formula of liquid microelement (B) component is shown in Table 9.
The formula of table 9 liquid microelement (B)
In the first run time construction, it is prepared for compounding activation agent B according to aforementioned proportion, wherein comprises with volume basis
Account for the pseudomonas fermentation liquid of the compounding activation total liquid phase volume of agent B 2.0%, by this compounding activation agent B according to construction
Well man-made fracture volume 2 times injection, injects into well from oil sets annular space, and use clear water as displacement fluid,
Replace clear water 15-20m3, wherein, clear water volume is wellbore volume, and Main Function is for by complete for compounding activation agent
Entirely head into stratum and man-made fracture region.Closing well normally produced after 4-5 days.In the construction of follow-up 3 rounds,
The injection cycle of every round is 1 month, and i.e. 30 days, the compounding activation agent used was that above-mentioned nutrient is water-soluble
Liquid and the mixture of 5 times of volumes of air, be not added with microbial bacterial agent.The viscosity of crude of this well, before measure
42.5mPa.s drop to 32.5mPa.s, viscosity break ratio is 23.5%.Visible, stimulate by injecting compounding activation agent
Microorganism is bred and metabolism in the body of crack, generates glycolipid class metabolite, can reduce the physical property of crude oil,
Realize solving plugging function, improve the flow conductivity of crack body.Meanwhile, after site operation often taken turns by this well, the 7th day all
Detecting, in production fluid, the concentration of injected strain is all 1 × 106More than CFU/ml, illustrates that microbial bacteria exists
Oil reservoir is survived and becomes indigenous flora.This experiment carries out microorganism de-plugging at this block, the effective well of de-plugging
16 mouthfuls, 16 mouthfuls of wells add up to increase oil 6303.1 tons, 162 days the longest effect duration.
Embodiment 5
On-the-spot test:The middle temperature experiment well of Jiangsu oilfield five mouthfuls, the master data of these five mouthfuls of wells is shown in Table 10.This five cause for gossip
Testing well and be distributed in same block, this block is the hypotonic common heavy oil reservoir of middle temperature, by fracturing production, goes into operation
After, increase with the development time, man-made fracture body pollution is serious, affects oil well and normally produces, therefore to this five
Microorganism de-plugging operation carried out by mouth well.
Table 10 experiment well master data
Use compounding activation agent B, wherein select bacillus subtilis (Bacillus subtilis ATCC21332)
As microorganism, this bacterium has good temperature tolerance through document report, and it is alive to produce ester peptides biological surface
Property agent surfactin, this bacterial strain can also utilize nitrate to carry out amphimicrobian breathing.This bacterial strain is prepared as bacterium
Bulk concentration is 1.0 × 108The fermentation liquid of CFU/ml is as microbial bacterial agent.
Described compounding activation agent B contains the micro-life accounting for the compounding activation total liquid phase volume of agent B 5.0% with volume basis
Thing microbial inoculum, the nutrient that compounding activation agent B contains is shown in Table 11 with denitrification electron acceptor aqueous solution composition.
Table 11 nutrient and denitrification electron acceptor aqueous solution constituent
Prepare compounding activation agent B according to aforementioned proportion, inject with 1.5 times of volumes of man-made fracture body, close after construction
Well drives a well after 3 days and normally produces, and after experiment, body flow conductivity part in crack is recovered, and oil well production increasing effect is notable
(being shown in Table 12).
12 5 mouthfuls of well effect of increasing production of table
Claims (7)
1. one kind utilizes the method that compounding activation agent improves fracturing well capacity, it is characterised in that:
Described method is that compounding activation agent A or compounding activation agent B introduce the fracturing fracture of formation at target locations and attached
Surface layer region, by propagation and the metabolic activity of activating microorganisms in formation at target locations, removes formation contaminant
Thing also generates beneficial metabolic product, the method improving fractured well production capacity；
Described method comprise the following steps at least one:
(1), after pressing crack construction, within every 5～10 days, it is to inject once-combined activator A or compound a cycle to swash
Live agent B, and the compounding activation agent A every time injected or the volume of compounding activation agent B are artificial crevice volume
1～2 times, carry out 3～5 cycles altogether；
(2) in normal productive process, when oil well liquid-producing index is less than normal production value more than 30%, press
Being a cycle to inject once-combined activator A or compounding activation agent B according to every 3～7 days, that injects answers every time
Close activator A or compounding activation agent B volume is artificial crevice volume 1～2 times, carries out 1～3 altogether
The individual cycle；
Wherein,
Described compounding activation agent A includes the nutrient solution that nutrient concentrations is 1.0～10wt%；
Described nutrient by weight, by 50～nitrogen source, 10～20% of the carbon source of 75%, 10～20%
The liquid microelement of inorganic salt, 2.5～5.0%, 2.5～5.0% somatomedin composition；
Described compounding activation agent A adds microbial bacterial agent, with the addition of the compounding activation agent A of microbial bacterial agent
Constitute compounding activation agent B；
In described compounding activation agent B containing with volume basis be the compounding activation total liquid phase volume of agent B 0.1%～
The microbial bacterial agent of 5.0%；
Described microbial bacterial agent is containing 1 × 107～1 × 1010The culture fluid of CFU/ml strain concentration.
Utilizing the method that compounding activation agent improves fracturing well capacity the most as claimed in claim 1, it is special
Levy and be:
Described compounding activation agent A is made up of electron acceptor and described nutrient solution；
Described electron acceptor one in aerobic electron acceptor, denitrification electron acceptor；
At least one in air, oxygen of described aerobic electron acceptor；
At least one in nitrate, nitrite of described denitrification electron acceptor.
Utilize the method that compounding activation agent improves fracturing well capacity, its feature the most as claimed in claim 2
It is:
When the electron acceptor positro receptor preferably contained in described compounding activation agent A, described nutrient
Solution positro receptor volume ratio of becoming reconciled is 1:(3～40)；
When the electron acceptor contained in described compounding activation agent A is denitrification electron acceptor, described anti-nitre
Changing electron acceptor weight concentration in compounding activation agent A is 1.0～5.0wt%.
Utilize the method that compounding activation agent improves fracturing well capacity, its feature the most as claimed in claim 3
It is:
When the electron acceptor positro receptor preferably contained in described compounding activation agent A, described nutrient
Solution positro receptor volume ratio of becoming reconciled is 1:(3～20)；
When the electron acceptor contained in described compounding activation agent A is denitrification electron acceptor, electron acceptor exists
Weight concentration in compounding activation agent A is 1.5～3.0wt%.
Utilize the method that compounding activation agent improves fracturing well capacity, its feature the most as claimed in claim 1
It is:
The described microorganism in microbial bacterial agent is selected from pseudomonas (Pseudomonas), bacillus cereus
(Bacillus), at least one in clostridium (Clsotridium)；
In described compounding activation agent B containing with volume basis be the compounding activation total liquid phase volume of agent B 1～
The microbial bacterial agent of 5.0%；
Described microbial bacterial agent is containing 1 × 107～1 × 109The culture fluid of CFU/ml strain concentration.
Utilize the method that compounding activation agent improves fracturing well capacity, its feature the most as claimed in claim 5
It is:
The described microorganism in microbial bacterial agent is selected from Pseudomonas aeruginosa (Pseudomonas
Aeruginosa), bacillus subtilis (Bacillus subtilis), Clostridium butyricum (Clsotridium butyricum)
With at least one in clostridium acetobutylicum (Clsotridium acetobutyricum).
7. the method for claim 1, it is characterised in that:
Described formation contaminant thing, including fracturing fluid residue, the waxiness of crude oil heavy component, colloid or drip
At least one in blue or green matter and formation water fouling；
Described beneficial metabolic product includes that gas, biosurfactant, small molecule solvent, little molecule have
At least one in machine acid；
In described beneficial metabolic product,
Described gas, including CO2、H2、CH4、N2In at least one；
Described biosurfactant, including glycolipid class, lipopeptid class, lipoprotein, sugar-protein-lipid complex
At least one of apoplexy due to endogenous wind；
Described small molecule solvent, including ethanol, propanol, isopropanol, butanol, butanediol, isoamyl alcohol,
At least one in acetone；
Described small molecular organic acid, including in formic acid, acetic acid, propanoic acid, butanoic acid, lactic acid, oxalic acid extremely
Few one.
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| CN107558968B (en) * | 2016-07-01 | 2019-08-09 | 中国石油化工股份有限公司 | A kind of method that oil well microbial compound throughput recovers the oil |
| CN107165611B (en) * | 2017-06-27 | 2019-04-02 | 中国石油化工股份有限公司 | A kind of method that the inefficient oil well microbial single well stimulation of low yield recovers the oil |
| CN109653719B (en) * | 2018-12-17 | 2020-02-07 | 中国石油大学(北京) | Method for improving fracturing effect of dense thick oil by using in-situ microorganisms |
| CN110513073B (en) * | 2019-08-23 | 2020-07-28 | 北京润世能源技术有限公司 | Sectional type activator injection mode for activating microorganisms in oil reservoir to generate plugging effect |
| CN110821461B (en) * | 2019-10-28 | 2021-11-30 | 中国石油化工股份有限公司 | Composite water lock releasing process for low-permeability oil well |
| CN113586027A (en) * | 2021-09-14 | 2021-11-02 | 北京科技大学 | Method for enhancing fracturing-oil displacement effect by using functional microorganisms |
| CN113917116B (en) * | 2021-09-29 | 2024-01-02 | 中国海洋石油集团有限公司 | Method for determining liquid extraction capacity of emulsified thickened oil of oil well |
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