CN103614127A - Microorganism and lipopeptide combined low-temperature oil reservoir oil extraction and paraffin removal and inhibition technology - Google Patents
Microorganism and lipopeptide combined low-temperature oil reservoir oil extraction and paraffin removal and inhibition technology Download PDFInfo
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- CN103614127A CN103614127A CN201310476793.XA CN201310476793A CN103614127A CN 103614127 A CN103614127 A CN 103614127A CN 201310476793 A CN201310476793 A CN 201310476793A CN 103614127 A CN103614127 A CN 103614127A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
- C09K8/524—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning organic depositions, e.g. paraffins or asphaltenes
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
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Abstract
The present invention discloses a microorganism and lipopeptide combined low-temperature oil reservoir oil extraction and paraffin removal and inhibition technology, which is characterized in that the selected microorganism is Leuconostoc mesenteroides subsp.mesenteroides, the preservation number is CGMCC NO.1.0544, the surfactant matched with the microorganism is a lipopeptide, the oil extraction formula comprises 5 g/L of glucose, 2 g/L of peptone, 0.3 g/L of potassium dihydrogen phosphate, 1.4 g/L of disodium hydrogen phosphate dodecahydrate and 0.25 g/L of magnesium sulfate heptahydrate, and water with a pH value of 7-8 is injected. The technology can be applied for the low-temperature oil reservoir with the temperature of 10-37 DEG C, and provides good effects of emulsification, viscosity reduction, and paraffin removal and inhibition for crude oil, especially for highly waxy crude oil, wherein the optimal application temperature is 18-27 DEG C.
Description
Technical field
The present invention relates to a kind of microorganism and tensio-active agent and recover the oil and clear anti-wax technique, especially relate to a kind of Leuconostoc mesenteroides and the oil recovery of lipopeptid combination low-temperature reservoir and clear anti-wax technique.
Background technology
The sixties in 20th century, China started Microbial Enhanced Oil Recovery to study, but slower development.At the end of the eighties, Daqing oil field has taken the lead in carrying out the underground fermentation test of microorganism (30 ℃) of two mouthfuls of wells.The oil field liquid such as huge port, triumph, long celebrating, the Liaohe River, Xinjiang had carried out single well stimulation test afterwards.The end of the year 2000, Dongxing oil fields in Shengli has been introduced the high temperature resistant bacterial classification of U.S. NPC company, at Y-16 well group, carry out high temperature resistant microbial oil displacement and improved recovery ratio research and test in place, result shows, recovery ratio reaches 43.41%, and Shengli Oil Field sieve 801 block inoculating microbe technology of reservoir sweep test in places improve recovery ratio 2.66%.North China Oilfield at 2007～2012 Ah 36 of Nian Erlian company fault blocks, breathe out the whole microorganism of 22 fault blocks, Bao Lige oil field and drive, on average improve recovery ratio 5% left and right, obtained good oil recovery effect.Microorganism Wax Removal And Paraffin Inhibition is the new ideas of carrying in recent years, utilizes the surface effects of bacterial body and meta-bolites thereof.Before wax crystallization is separated out, try to be the first and coat layer of surface promoting agent material on wax core surface, so just can stop wax in the adhering to of solid matter surface, reach the object that stops wax deposition.Utilize bacterium to stop the cohesion of paraffin also can play to the dissemination of wax and stop wax deposition effect.It is the important means of tertiary oil recovery that tensio-active agent recovers the oil, and it can pass through the reducing viscosity by emulsifying to crude oil, and the cleanup action of rock surface is improved to oil recovery factor.
But microbe oil production is limited by the temperature of oil reservoir, general microorganisms for oil recovery can only be applicable to the oil reservoir of 30～70 ℃, and temperature is higher than 70 ℃, or temperature is lower than 25 ℃, and microbe oil production will be very restricted.Yet the former oil properties of geology is very complicated, especially, in low temperature and the high stratum of wax content, microbe oil production and tensio-active agent recover the oil and all can be very limited.Most tensio-active agents are very unobvious to the clear wax control effect of high wax content oil, cause oil pick-up tube and transport line to stop up.
Summary of the invention
In order to overcome the application limitation of microbe oil production in low-temperature reservoir, better in order to overcome surfactant flooding, the clear undesirable difficulty of wax control effect, the invention provides a kind of microorganism and lipopeptid combination low-temperature reservoir recovers the oil and clear anti-wax technique.This technology is lipopeptid and the coupling of low temperature amphimicrobe, has the advantage of the clear wax control of the displacement of reservoir oil at low-temperature reservoir especially temperature in lower than 30 ℃ of oil reservoirs.
The technical solution adopted for the present invention to solve the technical problems is: the microbial strains of screening is the bright film beading of intestines bacterium (Leuconostoc mesenteroides subsp.mesenteroides), and its preserving number is the Leuconostoc mesenteroides of CGMCC NO1.0544; The tensio-active agent of screening is lipopeptid; The oil recovery culture medium prescription of optimizing is (g/L): glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25, the water that injection pH is 7～8; Lipopeptid microorganism combining bacteria liquid consumption 0.5～1.5%.Oil recovery injection mode be water injection well lipopeptid microorganism combining bacteria liquid with recover the oil with inject together with substratum or recovers the oil with substratum, first inject a part after injection together.
Preserving number is that the Leuconostoc mesenteroides of CGMCC NO1.0544 is bought and obtained from Chinese microorganism strain preservation management committee's common micro-organisms center.Applicant buys and obtains in April, 2013 15Xiang China Committee for Culture Collection of Microorganisms common micro-organisms center.
The present invention includes and adopt intestines bright film beading bacterium (Leuconostoc mesenteroides subsp.mesenteroides), its preserving number is CGMCC NO1.0544, this bacterial classification is facultative anaerobe, can be at 5～35 ℃ of growth and breedings, 18～25 ℃ of best production temperature ranges, can utilize the organism such as glucose, peptone to produce organic acid, Organic Alcohol gas etc., crude oil is had to good reducing viscosity by emulsifying effect, secondly microbial bacteria somatocyte have individual little, quantity is many, surface adsorption property is strong, breed the features such as fast, at crude oil, has good wax control effect clearly; Lipopeptid is a kind of novel bio-surfactant, and the emulsifying effectiveness that viscous crude and the high content of wax are had is obvious; This Leuconostoc mesenteroides to lipopeptid without Degradation; Adopting substratum is in order to make the microorganism can better growth and breeding metabolism in stratum, emulsified crude oil, and play the effect of wax control clearly.This system composition used is nontoxic, harmless, environmental protection, easily degraded, at low-temperature reservoir, there is good application prospect.
The invention has the beneficial effects as follows, microorganism and the application of tensio-active agent coupling in low-temperature reservoir have been realized, having solved traditional microorganism can not improve the usefulness of lipopeptid surfactant flooding at low-temperature reservoir especially in a difficult problem for the oil recovery of oil reservoir below 30 ℃, clear wax control, improves oil recovery factor.
Embodiment
Example 1
A kind of microorganism and lipopeptid combination low-temperature reservoir recover the oil and clear anti-Wax composition, preserving number is that the Leuconostoc mesenteroides of CGMCC NO.1.0544 coordinates with 0.5% lipopeptid, and its mixing ratio is: the volume ratio of Leuconostoc mesenteroides fermented liquid and 0.5% lipopeptid solution is 1:1; Oil recovery culture medium prescription is (g/L): glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25.
Lipopeptid and microorganism combination liquid are to 20 ℃ of emulsifications of Yanchang Oilfield crude oil, clear wax control test.
High certain the well oil sample of Yanchang Oilfield, detecting wax content is 18%, 20 ℃ of left and right of formation temperature, belong to the high content of wax, low-temperature reservoir.Shop experiment is in 250ml Erlenmeyer flask, to add respectively substratum 100ml, (culture medium prescription is g/L: glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25), crude oil 10ml, lipopeptid and the microorganism mixed solution 1ml of inoculation preparation, plastic bag sealing, 20 ℃, water-bath vibration 48h.Result oil sample is mixed into lark, and microscopic examination growth is good, 20 ℃ of standing 24h, crude oil does not lump, little oil droplet dispersed, proves that this lipopeptid microorganism mixed bacteria liquid is at 20 ℃, and Yanchang Oilfield waxy crude oil is had to good emulsification, clear wax control effect.
Example 2
A kind of microorganism and lipopeptid combination low-temperature reservoir recover the oil and clear anti-Wax composition, preserving number is that the Leuconostoc mesenteroides of CGMCC NO.1.0544 coordinates with 0.5% lipopeptid, and its mixing ratio is: the volume ratio of Leuconostoc mesenteroides fermented liquid and 0.5% lipopeptid solution is 1:1; Oil recovery culture medium prescription is (g/L): glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25.
Lipopeptid and microorganism combination liquid are to 30 ℃ of emulsifications of Yanchang Oilfield crude oil, clear wax control test.
High certain the well oil sample of Yanchang Oilfield, detecting wax content is 18%, shop experiment is in 250ml Erlenmeyer flask, to add respectively substratum 100ml, (culture medium prescription is g/L: glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25), crude oil 10ml, lipopeptid and the microorganism mixed solution 0.5ml of inoculation preparation, plastic bag sealing, 30 ℃, water-bath vibration 48h.Result oil sample is mixed into lark, and microscopic examination growth is good, 20 ℃ of standing 24h, crude oil does not lump, little oil droplet dispersed, proves that this lipopeptid microorganism mixed bacteria liquid is at 30 ℃, and Yanchang Oilfield waxy crude oil is had to good emulsification, clear wax control effect.
Example 3
Lipopeptid and microorganism combination liquid are to 18 ℃ of emulsifications of Yanchang Oilfield crude oil, clear wax control test.
High certain the well oil sample of Yanchang Oilfield, detecting wax content is 18%.Shop experiment is in 250ml Erlenmeyer flask, to add respectively substratum 100ml, (culture medium prescription is g/L: glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25), crude oil 10ml, lipopeptid and the microorganism mixed solution 1.5ml of inoculation preparation, plastic bag sealing, 15 ℃, water-bath vibration 48h.Result oil sample is mixed into lark, and microscopic examination growth is good, 18 ℃ of standing 24h, crude oil does not lump, little oil droplet dispersed, proves that this lipopeptid microorganism mixed bacteria liquid is at 18 ℃, and Yanchang Oilfield waxy crude oil is had to good emulsification, clear wax control effect.
Claims (2)
1. a microorganism and lipopeptid combination low-temperature reservoir recover the oil and clear anti-Wax composition, it is characterized in that: preserving number is that the Leuconostoc mesenteroides of CGMCC NO.1.0544 coordinates with 0.5% lipopeptid, and its mixing ratio is: the volume ratio of Leuconostoc mesenteroides fermented liquid and 0.5% lipopeptid solution is 1:1; Oil recovery culture medium prescription is (g/L): glucose 5, peptone 2, potassium primary phosphate 0.3, disodium hydrogen phosphate 1.4, magnesium sulfate heptahydrate 0.25.
2. the application of composition as claimed in claim 1, is applicable to the oil reservoir of 10～50 ℃, preferably 18～25 ℃.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106833579A (en) * | 2017-02-06 | 2017-06-13 | 天津中油科远石油工程有限责任公司 | A kind of novel microbial Wax removing agent |
| CN108117866A (en) * | 2016-11-30 | 2018-06-05 | 中国石油天然气股份有限公司 | Well flushing fluid, preparation method, use method and determination method thereof |
| CN111363530A (en) * | 2020-01-13 | 2020-07-03 | 西安石油大学 | Mud cake dispersant for increasing production of oil and gas wells |
| CN114427406A (en) * | 2020-09-23 | 2022-05-03 | 中国石油化工股份有限公司 | High-coagulation thickened oil shaft lifting biological treatment method |
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| CN1558085A (en) * | 2004-01-13 | 2004-12-29 | 天津南开戈德集团有限公司 | Microorganism oil production method |
| CN101153267A (en) * | 2007-07-11 | 2008-04-02 | 华东理工大学 | Microorganism powder preparation for oil production and method of use thereof |
| CN102504789A (en) * | 2011-11-03 | 2012-06-20 | 大庆华理能源生物技术有限公司 | Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil |
-
2013
- 2013-10-12 CN CN201310476793.XA patent/CN103614127A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1558085A (en) * | 2004-01-13 | 2004-12-29 | 天津南开戈德集团有限公司 | Microorganism oil production method |
| CN101153267A (en) * | 2007-07-11 | 2008-04-02 | 华东理工大学 | Microorganism powder preparation for oil production and method of use thereof |
| CN102504789A (en) * | 2011-11-03 | 2012-06-20 | 大庆华理能源生物技术有限公司 | Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117866A (en) * | 2016-11-30 | 2018-06-05 | 中国石油天然气股份有限公司 | Well flushing fluid, preparation method, use method and determination method thereof |
| CN108117866B (en) * | 2016-11-30 | 2020-11-03 | 中国石油天然气股份有限公司 | Well flushing fluid, preparation method, use method and determination method thereof |
| CN106833579A (en) * | 2017-02-06 | 2017-06-13 | 天津中油科远石油工程有限责任公司 | A kind of novel microbial Wax removing agent |
| CN111363530A (en) * | 2020-01-13 | 2020-07-03 | 西安石油大学 | Mud cake dispersant for increasing production of oil and gas wells |
| CN114427406A (en) * | 2020-09-23 | 2022-05-03 | 中国石油化工股份有限公司 | High-coagulation thickened oil shaft lifting biological treatment method |
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