CN106085902B - One plant of microbe oil production bacterium W-Y1 and its application - Google Patents
One plant of microbe oil production bacterium W-Y1 and its application Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 43
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 28
- 241000589187 Rhizobium sp. Species 0.000 claims description 38
- 239000003208 petroleum Substances 0.000 claims description 33
- 230000015556 catabolic process Effects 0.000 claims description 19
- 238000006731 degradation reaction Methods 0.000 claims description 19
- 239000000725 suspension Substances 0.000 claims description 18
- 238000011084 recovery Methods 0.000 claims description 16
- 239000002068 microbial inoculum Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000470 constituent Substances 0.000 claims description 4
- 241000589180 Rhizobium Species 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 11
- 230000006872 improvement Effects 0.000 abstract description 4
- 239000003921 oil Substances 0.000 description 37
- 239000000243 solution Substances 0.000 description 18
- 230000001580 bacterial effect Effects 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 7
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 229930195733 hydrocarbon Natural products 0.000 description 5
- 150000002430 hydrocarbons Chemical class 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 239000004215 Carbon black (E152) Substances 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000007483 microbial process Effects 0.000 description 4
- 229910017677 NH4H2 Inorganic materials 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 239000003209 petroleum derivative Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000010779 crude oil Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 241001308669 Rhizobium lemnae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003129 oil well Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/41—Rhizobium
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
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Abstract
The invention discloses one plant of microbe oil production bacterium W-Y1 and its applications.Microbe oil production bacterium W-Y1 of the invention is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 23rd, 2016, and deposit number is CGMCC No.12492.Be experimentally confirmed: microbe oil production bacterium W-Y1 of the invention not only has the function of degraded oil, can be used for the improvement and reparation of oil pollution, and can be also used for microbe oil production, improves tar productivity, has a good application prospect.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to one plant of microbe oil production bacterium W-Y1 and its application.
Background technique
Oil pollution is widely distributed, and it is various to be related to the various industry such as oil recovery, oil refining, chemical industry, machinery and Oil spills etc.
Pollute environment.Petroleum hydrocarbon complicated components multiplicity, hydrophobicity is strong and is difficult to be contacted by common micro-organisms, degrades, utilizes.
Microbial Enhanced Oil Recovery is exactly to utilize the growth of microorganism in oil reservoir by injecting nutrition or microorganism into stratum
Metabolic activity improves crude output and recovery ratio.Traditional petroleum function stem is located away from water body and soil more.It is existing to grind
Study carefully and shows microorganism except in addition to water body and soil, also there is extensive distribution in petroleum oil phase respectively, therefore from petroleum oil phase
Separate that relevant function stem repairs petroleum hydrocarbon contaminated improvement and Microbial Enhanced Oil Recovery is of great significance.
Summary of the invention
It is an object of the present invention to provide one plant of rhizobium Rhizobium sp.W-Y1.
The deposit number of rhizobium Rhizobium sp.W-Y1 provided by the invention is CGMCC No.12492.
It is a further object to provide above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its cultures
The new application of liquid or its tunning or the microbial inoculum containing it.
The present invention provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspensions or its culture solution or its fermentation to produce
Object or application containing its microbial inoculum in oil degradation.
The present invention also provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its culture solution or its fermentations
Product or application containing its microbial inoculum in the product for preparing oil degradation.
The present invention also provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its culture solution or its fermentations
Product or application containing its microbial inoculum in petroleum pollution and/or reparation.
The present invention also provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its culture solution or its fermentations
Product or application containing its microbial inoculum in the product for preparing petroleum pollution and/or reparation.
The present invention also provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its culture solution or its fermentations
Product or application containing its microbial inoculum in microbe oil production.
The present invention also provides above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its culture solution or its fermentations
Product or application containing its microbial inoculum in the product for preparing microbe oil production.
It is a still further object of the present invention to provide a kind of products.
The active constituent of product provided by the invention be above-mentioned rhizobium Rhizobium sp.W-Y1 or its bacteria suspension or its
Culture solution or its tunning or the microbial inoculum containing it；
The product has following 1) -3) in any function:
1) oil degradation；
2) petroleum pollution and/or reparation；
3) microbe oil production.
It is a still further object of the present invention to provide a kind of petroleum pollution and/or the methods of reparation.
Petroleum pollution provided by the invention and/or the method for reparation include the following steps: with above-mentioned rhizobium
Rhizobium sp.W-Y1 or its bacteria suspension handle petroleum pollution.
Final object of the present invention is to provide a kind of method of microbe oil production.
The method of microbe oil production provided by the invention includes the following steps: during oil recovery, and above-mentioned root nodule is added
Bacterium Rhizobium sp.W-Y1 or its bacteria suspension.
The present invention provides a kind of microbe oil production bacterium W-Y1, it is micro- which has been preserved in China on May 23rd, 2016
Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.12492.It is experimentally confirmed: this hair
Bright microbe oil production bacterium W-Y1 not only has the function of degraded oil, can be used for the improvement and reparation of oil pollution, but also
It can be used for microbe oil production, improve tar productivity, have a good application prospect.
Detailed description of the invention
Fig. 1 is the phylogenetic tree based on W-Y1 bacterial strain 16S rDNA.
Fig. 2 is total hydrocarbon change of component before and after degraded oil.
Fig. 3 is object mould displacement of reservoir oil result curve.
Preservation explanation
Strain name: rhizobium
Latin name: Rhizobium sp.
Strain number: W-Y1
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on May 23rd, 2016
Collection is registered on the books number: CGMCC No.12492
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Quantitative test in following embodiments, is respectively provided with three repeated experiments, and results are averaged.
The separation and identification of embodiment 1, W-Y1 bacterial strain
1, the separation of W-Y1 bacterial strain
1g crude oil (in March, 2015 is derived from Kelamayi Xinjiang Oilfield oil well crude oil) is taken to be added to 121 DEG C of sterilizing 30min's
Liquid inorganic salt culture medium (5g/L NaCl, 1g/L NH4H2PO4、1g/L(NH4)2SO4、1g/L K2HPO4、3g/L KNO3, pH
In 7-8), 37 DEG C, after 150rpm shaken cultivation 30d, dilution spread is in LB culture medium (10g/L NaCl, 10g/L peptone, 5g/
L yeast powder, pH 7-8) on plate, after cultivating 7d, the scribing line of picking single colonie saves after purification, and will isolate and purify point of acquisition
W-Y1 bacterial strain is named as from object.
2, the Molecular Identification of W-Y1 bacterial strain
By W-Y1 strain inoculated in LB liquid medium (10g/L NaCl, 10g/L peptone, 5g/L yeast powder, pH 7-
8) in, 30 DEG C, 150rpm shaken cultivation for 24 hours.Take 4 DEG C of 1ml fresh cultured bacterium solution, 8000rpm centrifugation 5min, collect thallus in
In 2ml centrifuge tube, DNA is extracted using DNA extraction kit.After electrophoresis detection, PCR expansion is carried out using universal primer 8F/1492R
Increase, obtains the PCR product containing the conserved region W-Y1 bacterial strain 16S rDNA.Primer sequence is as follows:
8F:5 '-AGA GTTTGATCC TGGCTCAG-3 '；
1492R:5 '-GGTTACCTTGTTACGACTT-3 '.
Electrophoresis detection PCR product is simultaneously sequenced, and sequencing result shows: the nucleotide sequence such as sequence of W-Y1 bacterial strain 16S rDNA
Shown in 1.It is as shown in Figure 1 using software MEGA phylogenetic tree construction based on W-Y1 bacterial strain 16S rDNA sequence.Sequence 1 is mentioned
Hand over GenBank, according to blast search and homology analysis comparison result show W-Y1 bacterial strain 16S rDNA sequence with it is known
The highest similitude of strain Rhizobium lemnae is only 95.73%, shows that the bacterium is a kind of newfound new bacterium.
In summary qualification result, W-Y1 strain name are rhizobium, and classification naming is Rhizobium sp., the bacterium
Strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as on May 23rd, 2016
CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101), it protects
Hiding number is CGMCC No.12492.
The application of embodiment 2, W-Y1 bacterial strain in petroleum pollution reparation
1, the acquisition of W-Y1 bacterium solution
By W-Y1 strain inoculated in LB liquid medium (10g/L sodium chloride, 10g/L peptone, 5g/L yeast powder, pH 7-
8) in, 30 DEG C, 150rpm shaken cultivation for 24 hours, obtain W-Y1 bacterium solution (W-Y1 bacterial concentration be 108-109CFU/ml)。
2, W-Y1 bacterium solution degraded oil
The W-Y1 bacterium solution that step 1 obtains is inoculated in petroleum minimal medium according to the ratio that volume fraction is 5%
(1g/L petroleum, 5g/L NaCl, 1g/L NH4H2PO4、1g/L(NH4)2SO4、1g/L K2HPO4、3g/L KNO3, pH 7-8) in
(concentration of W-Y1 bacterium solution is 106-107CFU/ml), 30 DEG C, 150rpm shaken cultivation 7d, obtain W-Y1 catabolite.
3, by the stone in the method measurement W-Y1 catabolite in document " separation of oil degradation bacterium and its degradation property "
Oily residual volume, and calculate petroleum degradation rate.The formula of petroleum degradation rate: (petroleum additional amount-petroleum participation amount)/petroleum is added
Amount.Calculated result shows: the degradation rate of petroleum is 58.6%.
4, W-Y1 is measured by professional standard SY/T5779-2008 petroleum and depositional organic matter hydro carbons gas chromatography analysis method
The total hydrocarbon change of component of bacterium solution degradation front and back.
As a result as shown in Figure 2, wherein black map is petroleum the hydrocarbon component before degrading, and red map is petroleum hydrocarbon after degradation
Component.Figure it is seen that petroleum the hydrocarbon component significantly reduces after W-Y1 is acted on, illustrate that W-Y1 bacterial strain has degraded oil
Function, can be used for the improvement and reparation of oil pollution.
Embodiment 3, the application of W-Y1 bacterial strain in microbial oil extraction technology
The oil recovery effect of microorganism fungus kind is evaluated by indoor object mould oil displacement experiment, specific experimental method is as follows:
1, according to (600 μm of oil reservoir block permeability2), using High Pressure Model pipe (specificationIt purchases to Hai'an
Petroleum scientific research Instrument Ltd.) in load different meshes quartz sand be made as sandpack column pipe；
2, using permeability measuring apparatus measurement model pipe permeability, the model pipe met, model pipe back-up sand parameter are selected
As shown in table 1；
Table 1, model pipe back-up sand parameter
3, saturation water flooding is vacuumized, porosity is calculated；
4, oily expelling water establishes irreducible water, measures initial oil saturation；And aging 3 days under reservoir temperature (37 DEG C)；
5, the displacement of reservoir oil is carried out with injection water, moisture content (98%) stops water drive until outlet production fluid reaches capacity, and calculates water drive
Recovery ratio；
6, by W-Y1 strain inoculated in LB liquid medium (10g/L sodium chloride, 10g/L peptone, 5g/L yeast powder, pH
In 7-8), 30 DEG C, 150rpm shaken cultivation for 24 hours, obtain W-Y1 bacterium solution (concentration of W-Y1 bacterium solution be 108-109CFU/ml).It is infusing
Enter and NH is added in water4H2PO4、(NH4)2SO4、K2HPO4、KNO3, peptone, yeast powder and W-Y1 bacterium solution, mix, obtain containing W-Y1
Mixed liquor, making concentration of each component in the mixed liquor containing W-Y1 is respectively 1g/L NH4H2PO4、1g/L(NH4)2SO4、1g/L
K2HPO4、3g/L KNO3, 2g/L peptone, 1g/L yeast powder, 5% (volume fraction) W-Y1 bacterium solution.W- in the mixed liquor of W-Y1
The concentration of Y1 is 106-107CFU/ml。
It 7, will be in the above-mentioned mixed liquor injection model pipe containing W-Y1 by 0.5PV injection rate；It is trained under reservoir temperature (37 DEG C)
It supports 15 days；
8, secondary water drive, water drive to aqueous 98% or more, calculate separately note microorganism (mixed liquor containing W-Y1) during,
Microorganism (mixed liquor containing W-Y1) acts on the recovery ratio of subsequent waterflooding and W-Y1 bacterial strain improves recovery ratio.
In water drive, note microbial process, result such as 2 institute of table of the recovery ratio of different phases such as microbial action subsequent waterflooding
Show.To in water drive, note microbial process, the recovery ratio of the different phases such as microbial action subsequent waterflooding carried out comparison discovery:
It is recovered the oil using W-Y1 bacterial strain, recovery ratio improves 6.05%.Object mould displacement of reservoir oil result curve is as shown in Figure 3.
Table 2, water drive, in note microbial process, the recovery ratio of the different phases such as microbial action subsequent waterflooding
| Object mode step section | Recovery ratio (%) | It improves recovery ratio (%) |
| Water drive | 39,74 | / |
| Infuse microbial process | 41.45 | 1.71 |
| Microbial action subsequent waterflooding | 45.79 | 6.05 |
Claims (24)
1. one plant of rhizobium Rhizobium sp. W-Y1, deposit number is CGMCC No.12492.
2. the application of rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension in oil degradation.
3. application of the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 in oil degradation.
4. application of the microbial inoculum containing rhizobium Rhizobium sp. W-Y1 described in claim 1 in oil degradation.
5. rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension are in the product for preparing oil degradation
Application.
6. the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 is in the product for preparing oil degradation
Using.
7. the microbial inoculum containing rhizobium Rhizobium sp. W-Y1 described in claim 1 is in the product for preparing oil degradation
Application.
8. rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension in petroleum pollution and/or are repaired
Application in multiple.
9. the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 is in petroleum pollution and/or reparation
In application.
10. the microbial inoculum containing rhizobium Rhizobium sp. W-Y1 described in claim 1 in petroleum pollution and/or is repaired
Application in multiple.
11. rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension prepare petroleum pollution and/
Or the application in the product repaired.
12. the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 prepare petroleum pollution and/or
Application in the product of reparation.
13. containing rhizobium Rhizobium sp. W-Y1 described in claim 1 microbial inoculum prepare petroleum pollution and/
Or the application in the product repaired.
14. the application of rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension in microbe oil production.
15. application of the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 in microbe oil production.
16. application of the microbial inoculum containing rhizobium Rhizobium sp. W-Y1 described in claim 1 in microbe oil production.
17. rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacteria suspension are in the production for preparing microbe oil production
Application in product.
18. the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1 is in the product for preparing microbe oil production
In application.
19. the microbial inoculum containing rhizobium Rhizobium sp. W-Y1 described in claim 1 is in the production for preparing microbe oil production
Application in product.
20. a kind of product, active constituent is rhizobium Rhizobium sp. W-Y1 described in claim 1 or its bacterium is outstanding
Liquid；
The product has following 1) -3) in any function:
1) oil degradation；
2) petroleum pollution and/or reparation；
3) microbe oil production.
21. a kind of product, active constituent is the culture solution of rhizobium Rhizobium sp. W-Y1 described in claim 1；
The product has following 1) -3) in any function:
1) oil degradation；
2) petroleum pollution and/or reparation；
3) microbe oil production.
22. a kind of product, active constituent is the bacterium containing rhizobium Rhizobium sp. W-Y1 described in claim 1
Agent；
The product has following 1) -3) in any function:
1) oil degradation；
2) petroleum pollution and/or reparation；
3) microbe oil production.
23. a kind of petroleum pollution and/or the method for reparation include the following steps: with rhizobium described in claim 1
Rhizobium sp. W-Y1 or its bacteria suspension handle petroleum pollution.
24. a kind of method of microbe oil production, includes the following steps: during oil recovery, it is added described in claim 1
Tumor bacterium Rhizobium sp. W-Y1 or its bacteria suspension.
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| CN109306333B (en) * | 2018-10-25 | 2020-06-12 | 北京润世能源技术有限公司 | Microbial oil recovery bacterium W-Y8 and application thereof |
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| JP2008289445A (en) * | 2007-05-28 | 2008-12-04 | Tosoh Corp | Petroleum-decomposing microorganism, microorganism consortium, and method for cleaning petroleum-contaminated soil by using the same |
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