CN101131082A - Underground microbial oil production method - Google Patents

Underground microbial oil production method Download PDF

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Publication number
CN101131082A
CN101131082A CNA2006100304464A CN200610030446A CN101131082A CN 101131082 A CN101131082 A CN 101131082A CN A2006100304464 A CNA2006100304464 A CN A2006100304464A CN 200610030446 A CN200610030446 A CN 200610030446A CN 101131082 A CN101131082 A CN 101131082A
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China
Prior art keywords
oil
oil field
microbe
carry out
bacteria
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CNA2006100304464A
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Inventor
翁天波
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Shanghai CNPC Enterprise Group Co Ltd
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Shanghai CNPC Enterprise Group Co Ltd
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Priority to CNA2006100304464A priority Critical patent/CN101131082A/en
Publication of CN101131082A publication Critical patent/CN101131082A/en
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Abstract

The present invention relates to microbial petroleum producing technology, and is especially one underground microbial petroleum producing process. The process includes the following steps: sampling microbes from oil field sewage, oil field sludge, oil field silt and other oil field matter; enriching and separating in HUNGATE anaerobic mode to separate out single types of bacteria, culturing and anaerobic treating for further purification and domestication, physiological and biochemical index and metabolite analysis and testing, and analysis in the effect of bacteria on raw oil to determine the screened out bacteria; extruding the bacteria liquid into the stratum, compounding microbe diluting liquid with oil field sewage, and propagating microbe to complete microbe establishing process before producing from the oil well.

Description

A kind of ground laxative remedy microbe oil production method
Technical field
The present invention relates to a kind of microbe oil production method technical field.
Background technology
Microbe oil production is to utilize the useful activity of microorganism self such as degrading crude oil and/or metabolite such as biosurfactant, organic acid, alcohol to wait the oil recovery technique that improves oil recovery factor.
U.S. 1991 microbe oil production classify that heat is driven as, the 4th class after the chemical flooding, gas drive improves the oil recovery factor method, and in many oil fields, used, the former Soviet Union also classifies microbe oil production as new raising recovery ratio method that a kind of industrialness is used, states such as East Bloc, Australia, Canada also take much count of the research to MEOR, and achievement in research is used for mining site.Microbe oil production is with its considerable economic, the attention that unique advantage and vast potential for future development cause various countries' petroleum industrial circle.After the nineties in 20th century, especially in recent years, China also began microbe oil production Study on Technology and application work.Some institution of higher learning such as Shandong University, Nankai University etc. in succession with cooperation development such as Shengli Oil Field, Daqing oil field, Dagang Oilfields this technical research.Jilin Oil Field is being used the nutraceutical method of injection and is being carried out individual well microorganism huff and puff field test, Dagang Oilfield is carrying out the microbial oil displacement pilot test, Daqing oil field mainly carries out a large amount of fundamental research work at present, and Shengli Petroleum Administration Bureau has set up microbe oil production technical research centre of development specially, on the basis of laboratory test research, carrying out the application study of single well stimulation field test, simultaneously also in bacterial screening that carries out microbial oil displacement and relevant laboratory test.
No matter broad sense still is narrow sense is understood the microbe oil production technology, and the core of its understanding mainly is the difference of microbial fermentation processes and the difference in the field of enforcement, can be divided into ground method and ground laxative remedy.
The ground laxative remedy is that microorganism is injected the stratum, makes it produce various metabolites on the spot in oil reservoir.As long as supply with the enough nutriments of microorganism, the formation speed of metabolite will be greater than the speed of microbial degradation.Therefore, the ground laxative remedy has crude oil effect of increasing production longer duration and the low advantage of construction cost and being widely adopted.The indication microbe oil production mainly is meant the ground laxative remedy at present.
Summary of the invention
The purpose of this invention is to provide a kind of concrete microbe oil production method, the construction of this kind method is simple, and little to environmental change, compliance is strong.
For achieving the above object, the technical scheme taked of the present invention is:
A kind of ground laxative remedy microbe oil production method, the concrete steps of this method are: take a sample from oil field environment such as oilfield sewage, oil field anaerobically digested sludge, mud and silt, adopt HUNGATE anaerobism mode to carry out enrichment, separation respectively, the single cultivation of bacterium process, the Anaerobic Treatment of planting that is separated, be further purified, tame, carry out the analytical test of physiological and biochemical index and metabolite at last, and carry out the analysis of bacterium, determine the bacterial classification of screening the direct action effect of crude oil; Field by using fixed tubular column sleeve pipe squeeze note bacterium liquid utilizes oilfield treatments sewage preparing microorganism dilution to the stratum, guarantee the breeding time of microorganism and finish processor establishment before oil well drives a well.
At present, separate the natural oil recovery bacterium that obtains abroad and mainly contain pseudomonad, bacillus, micrococcus luteus, excellent bacillus, mycobacterium, arthrobacterium, clostridium, methagen, bacteroid, hot anaerobic bacteria etc.
Beneficial effect of the present invention: from comprehensive spot effect and laboratory inner evaluation result, the suitable using microbe oil recovery technique of shallow-layer common heavy oil reservoir, high pressure-temperature low-permeability sandstone oil reservoir then should not be used this technology.The injection that North China Oilfield selects 46 mouthfuls of wells the to carry out microbial inoculum experiment of handling up amounts to and injects 206 rounds, totally injects microorganism 28.8t.Wherein can't carry out the evaluation of result because of other measures and accident impact are arranged except that 16 mouthfuls of wells, 30 mouthfuls of actual accumulative totals of well increase oily 6765t, fall liquid 3181m3, average moisture decline 2.5%.Existing oil recovery factory, river of Shengli Oil Field 1996 and U.S. NPC microbial technique company carry out cooperation, and (existing He Zhuan, Hao Jia, Wang Gang, Shi Nan, Cao Qiao) carried out the compatibility test work of microbe oil production technology at 5 dissimilar oil reservoirs.The oil field is estimated by the feasibility of the suitable microorganism fungus kind of screening formation condition (temperature is at 90 ℃~100 ℃) the using microbe oil recovery technique special to this oil field and is affirmed in the literary composition, and has obtained microorganism fungus kind targetedly.
The specific embodiment
The present invention is further illustrated below in conjunction with the specific embodiment:
Bacterial classification is selected:
The selection of bacterial classification comprises screening of nature oil recovery bacterium and the cultivation of genetic engineering oil recovery bacterium.The existing natural oil recovery bacterium that at present abroad is applied to recover the oil also has genetic engineering oil recovery bacterium.Nature oil recovery bacterium is often taken a sample from oil field environment such as oilfield sewage, oil field anaerobically digested sludge, mud silt etc., adopt HUNGATE anaerobism mode to carry out enrichment, separation respectively, the single cultivation of bacterium process, the Anaerobic Treatment of planting that is separated, be further purified, tame, the analytical test of physiological and biochemical index and metabolite, and carry out the analysis of bacterium to the direct action effect of crude oil.At present, separate the natural oil recovery bacterium that obtains abroad and mainly contain pseudomonad, bacillus, micrococcus luteus, excellent bacillus, mycobacterium, arthrobacterium, clostridium, methagen, bacteroid, hot anaerobic bacteria etc.
The mechanism different according to microorganism, the application form of MEOR, oil field, Nanyang, Henan is improved recovery test with homemade Xanthan gun through having carried out the water injection well profile control with the cross-linking modified processing of trivalent chromic ion, the result shows, the intake profile of water injection well obtains adjusting, obtained tangible oil increasing precipitation effect, improved original recovery ratio 8%~12% with the comparable water drive oil of the Xanthan gun displacement of reservoir oil.1996~1997 years, the injection that North China Oilfield selects 46 mouthfuls of wells the to carry out microbial inoculum experiment of handling up amounted to and injects 206 rounds, totally injects microorganism 28.8t.Wherein can't carry out the evaluation of result because of other measures and accident impact are arranged except that 16 mouthfuls of wells, 30 mouthfuls of actual accumulative totals of well increase oily 6765t, fall liquid 3181m3, average moisture decline 2.5%.Existing oil recovery factory, river of Shengli Oil Field 1996 and U.S. NPC microbial technique company carry out cooperation, and (existing He Zhuan, Hao Jia, Wang Gang, Shi Nan, Cao Qiao) carried out the compatibility test work of microbe oil production technology at 5 dissimilar oil reservoirs.Adopt fixed tubular column sleeve pipe squeeze note bacterium liquid in the field trial to the stratum, utilize oilfield treatments sewage preparing microorganism dilution, so that microorganism reaches near the oil well well and outwards migration voluntarily, guarantee the breeding time of microorganism and before oil well drives a well, finish processor establishment.Result of the test shows, the integrated application of well piece of finishing experiment is effective, and from comprehensive spot effect and laboratory inner evaluation result, the suitable using microbe oil recovery technique of shallow-layer common heavy oil reservoir, high pressure-temperature low-permeability sandstone oil reservoir then should not be used this technology.
The oil field is estimated by the feasibility of the suitable microorganism fungus kind of screening formation condition (temperature is at 90 ℃~100 ℃) the using microbe oil recovery technique special to this oil field and is affirmed in the literary composition, and has obtained microorganism fungus kind targetedly.

Claims (1)

1. ground laxative remedy microbe oil production method, the concrete steps of this method are: take a sample from oil field environment such as oilfield sewage, oil field anaerobically digested sludge, mud and silt, adopt HUNGATE anaerobism mode to carry out enrichment, separation respectively, the single cultivation of bacterium process, the Anaerobic Treatment of planting that is separated, be further purified, tame, carry out the analytical test of physiological and biochemical index and metabolite at last, and carry out the analysis of bacterium, determine the bacterial classification of screening the direct action effect of crude oil; Field by using fixed tubular column sleeve pipe squeeze note bacterium liquid utilizes oilfield treatments sewage preparing microorganism dilution to the stratum, guarantee the breeding time of microorganism and finish processor establishment before oil well drives a well.
CNA2006100304464A 2006-08-25 2006-08-25 Underground microbial oil production method Pending CN101131082A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101818634A (en) * 2010-04-23 2010-09-01 中国石油化工股份有限公司 Method for regulating and controlling microbial community for oil extraction
CN102900411A (en) * 2012-10-29 2013-01-30 河南理工大学 Biological permeability-increasing method for coal reservoir
CN106479911A (en) * 2016-07-19 2017-03-08 桂林理工大学 Anaerobic degradation processes the cultural method of the Clostridium strain YB 6 of oil extraction waste water
CN112796720A (en) * 2020-12-30 2021-05-14 大庆油田有限责任公司 Method for improving recovery ratio of low-permeability reservoir by applying microorganisms

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101818634A (en) * 2010-04-23 2010-09-01 中国石油化工股份有限公司 Method for regulating and controlling microbial community for oil extraction
CN101818634B (en) * 2010-04-23 2013-04-03 中国石油化工股份有限公司 Method for regulating and controlling microbial community for oil extraction
CN102900411A (en) * 2012-10-29 2013-01-30 河南理工大学 Biological permeability-increasing method for coal reservoir
CN106479911A (en) * 2016-07-19 2017-03-08 桂林理工大学 Anaerobic degradation processes the cultural method of the Clostridium strain YB 6 of oil extraction waste water
CN112796720A (en) * 2020-12-30 2021-05-14 大庆油田有限责任公司 Method for improving recovery ratio of low-permeability reservoir by applying microorganisms

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Application publication date: 20080227