CN109234201A - One plant of microbe oil production bacterium W-Y10 and its application - Google Patents
One plant of microbe oil production bacterium W-Y10 and its application Download PDFInfo
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- CN109234201A CN109234201A CN201811252377.0A CN201811252377A CN109234201A CN 109234201 A CN109234201 A CN 109234201A CN 201811252377 A CN201811252377 A CN 201811252377A CN 109234201 A CN109234201 A CN 109234201A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 61
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 239000003921 oil Substances 0.000 claims abstract description 54
- 239000010779 crude oil Substances 0.000 claims abstract description 10
- 241000099088 Aquamicrobium sp. Species 0.000 claims description 39
- 239000003208 petroleum Substances 0.000 claims description 36
- 239000002068 microbial inoculum Substances 0.000 claims description 34
- 239000000725 suspension Substances 0.000 claims description 31
- 238000011084 recovery Methods 0.000 claims description 17
- 230000015556 catabolic process Effects 0.000 claims description 16
- 238000006731 degradation reaction Methods 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 12
- 238000004945 emulsification Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 2
- 239000003344 environmental pollutant Substances 0.000 claims description 2
- 231100000719 pollutant Toxicity 0.000 claims description 2
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- 230000006872 improvement Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 27
- 239000000243 solution Substances 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 238000006073 displacement reaction Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 241000067641 Aquamicrobium aestuarii Species 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
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- 238000002474 experimental method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
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- 235000019319 peptone Nutrition 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- CLWAXFZCVYJLLM-UHFFFAOYSA-N 1-chlorohexadecane Chemical compound CCCCCCCCCCCCCCCCCl CLWAXFZCVYJLLM-UHFFFAOYSA-N 0.000 description 2
- 241000982655 Aquamicrobium Species 0.000 description 2
- 241000982650 Aquamicrobium defluvii Species 0.000 description 2
- TXVHTIQJNYSSKO-UHFFFAOYSA-N BeP Natural products C1=CC=C2C3=CC=CC=C3C3=CC=CC4=CC=C1C2=C34 TXVHTIQJNYSSKO-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229910017677 NH4H2 Inorganic materials 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
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- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 241001625513 Alteromonas sp. P127 Species 0.000 description 1
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- 230000032683 aging Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003129 oil well Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
- 241000556533 uncultured marine bacterium Species 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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Abstract
The invention discloses one plant of microbe oil production bacterium W-Y10 and its applications.Microbe oil production bacterium W-Y10 of the invention was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 26th, 2018, and deposit number is CGMCC No.16533.Be experimentally confirmed: microbe oil production bacterium W-Y10 of the invention can emulsified crude oil, cannot be only used for microbe oil production, improve tar productivity, it may also be used for the improvement and reparation of oil pollution have a good application prospect.
Description
Technical field
The present invention relates to Microbial Enhanced Oil Recovery fields, and in particular to one plant of microbe oil production bacterium W-Y10 and its application.
Background technique
Oil pollution is widely distributed, and it is various to be related to the various industry and Oil spills etc. such as oil recovery, oil refining, chemical industry, machinery
Pollute environment.Petroleum hydrocarbon complicated components multiplicity, hydrophobicity is strong and is difficult to be contacted by common micro-organisms, degrades, utilizes.It is micro-
Biological oil recovery technique be exactly by injecting nutrition or microorganism into stratum, using the growth metabolism activity of microorganism in oil reservoir,
Improve crude output and recovery ratio.Traditional petroleum function stem separates water body, in soil more.It is existing studies have shown that micro- life
Object removes respectively in water, soil China and foreign countries, also has extensive distribution in petroleum oil phase, therefore separate relevant function from petroleum oil phase
Bacterial strain is of great significance in microbial oil extraction technology.
Hyun MiJin etc. is named as by concentration and separation to one plant at the seabeach of crude oil pollution
The new bacterium of Aquamicrobiumaestuarii, but it is not made whether the detection with degrading crude oil function
(Aquamicrobiumaestuarii sp.nov.,a marine bacterium isolated from a tidal
flat).Take benzo (a) pyrene degradation bacteria (the coal pollution for having separated one plant of entitled Aquamicrobiumlusatiense such as sparkling
The separation identification of benzo (a) pyrene degradation bacteria in area's soil).Wang Jianning etc. separated one plant it is entitled
Chloro-hexadecane degradation bacteria (the multiplicity of chloro-hexadecane degradation bacteria in arctic top layer seawater of Aquamicrobiumaerolatum
Property analysis and Alteromonas sp.P127 genome and degrading polycyclic aromatic hydrocarbons Mechanism Study).Andreoni etc. has separated one
Non-degradable bacterium (the Bacterial communities and enzyme of the entitled Aquamicrobiumdefluvium of strain
activities of PAHs polluted soils).Wang X etc. separated one plant it is entitled
Petroleum hydrocarbon (luxuriant and rich with fragrance and pyrene) degradation bacteria (Draft genome sequence of of Aquamicrobiumdefluvii
Aquamicrobiumdefluvii strain w13z1,a psychrotolerant halotolerant
Hydrocarbon-degrading bacterium and Draft genome sequence of halotolerant
polycyclic aromatic hydrocarbon-degrading Pseudomonas bauzanensis strain
W13Z2)。
Summary of the invention
It is an object of the present invention to provide one plant of Aquamicrobium sp.W-Y10.
The deposit number of Aquamicrobium sp.W-Y10 provided by the invention is CGMCC No.16533, classification life
It is common to be preserved in China Committee for Culture Collection of Microorganisms on September 26th, 2018 by entitled Aquamicrobium sp.
Microorganism center (abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101).
It is a further object to provide Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or
The new application of its tunning or the microbial inoculum containing it.
The present invention provides Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in petroleum recovering.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing petroleum recovering.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in microbe oil production.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing microbe oil production.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in emulsification of crude oil.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing emulsification of crude oil.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in oil degradation.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing oil degradation.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in petroleum pollution.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing petroleum pollution.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or containing its microbial inoculum oil pollution reparation in application.
The present invention also provides Aquamicrobium sp.W-Y10 or its bacteria suspensions or its culture solution or its tunning
Or containing its microbial inoculum preparation oil pollution reparation product in application.
It is a further object to provide a kind of products.
The active constituent of product provided by the invention be above-mentioned Aquamicrobium sp.W-Y10 or its bacteria suspension or its
Culture solution or its tunning or the microbial inoculum containing it；
The product has following 1) -6) in any function:
1) petroleum recovering；
2) microbe oil production；
3) emulsification of crude oil；
4) oil degradation；
5) petroleum pollution；
6) oil pollution reparation.
It is a still further object of the present invention to provide a kind of methods of microbe oil production.
The method of microbe oil production provided by the invention includes the following steps: during oil recovery, is added above-mentioned
Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or its tunning or the microbial inoculum containing it.
Final object of the present invention is to provide a kind of method of petroleum pollution and/or reparation.
Petroleum pollution provided by the invention and/or the method for reparation include the following steps: with above-mentioned
Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or its tunning or containing its microbial inoculum handle petroleum
Pollutant.
In above-mentioned application or product or method, the microbial inoculum the preparation method is as follows: by Aquamicrobium sp.W-
Y10 CGMCC No.16533, which is seeded in culture medium, to be cultivated, and the microbial inoculum is obtained.Bacteria concentration in the microbial inoculum is not less than
108cfu/mL。
The present invention provides a kind of microbe oil production bacterium W-Y10, classification naming is Aquamicrobium sp., the bacterium
Strain was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on September 26th, 2018
For CGMCC No.16533.Be experimentally confirmed: microbe oil production bacterium W-Y10 of the invention can emulsified crude oil, not only can be used
In microbe oil production, tar productivity is improved, it may also be used for the improvement and reparation of oil pollution have a good application prospect.
Detailed description of the invention
Fig. 1 is W-Y10 phylogenetic tree.
Fig. 2 is bacterial strain phylogenetic tree of the similitude 97% or more.
Fig. 3 is that W-Y10 emulsifies viscous crude.The left side is experimental group W-Y10, and the right is control group.
Fig. 4 is object mould displacement of reservoir oil result curve.
Preservation explanation
Latin name: Aquamicrobium sp.
Strain number: W-Y10
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on September 26th, 2018
Collection is registered on the books number: CGMCC No.16533
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Embodiment 1, the separation of W-Y10 bacterial strain, identification and preservation
One, the separation of W-Y10 bacterial strain
Oily phase petroleum, inventor are obtained from the oil well produced liquid of the North China Oilfield of Inner Mongol Erlianhaote in May, 2017
Therefrom isolated one plant of bacterium, is named as bacterial strain W-Y10.
Two, the identification of W-Y10 bacterial strain
1, the Morphological Identification of W-Y10 bacterial strain
The morphological feature of W-Y10 bacterial strain: the wet raised colour of loess of diameter 2mm, round rule is formed on LB solid medium
Color bacterium colony.
2, the Physiology and biochemistry identification of W-Y10 bacterial strain
The physiological and biochemical property of W-Y10 bacterial strain: it can be grown under 30 DEG C, 1% sodium chloride, but under 5% sodium chloride not
It can growth.K-IAO and PEARLITOL 25C cannot be utilized.
3, the Molecular Identification of W-Y10 bacterial strain
By the W-Y10 strain inoculated of activation, in LB liquid medium, (solvent is water, and solute and its concentration are respectively 10g/L
NaCl, 10g/L peptone, 5g/L yeast powder, pH7-8) in, 35 DEG C, 150rpm shaken cultivation for 24 hours.Take 1mL fresh cultured bacterium solution
4 DEG C, 8000rpm centrifugation 5min, collect thallus in 2mL centrifuge tube, extract DNA using DNA extraction kit.Electrophoresis detection
Afterwards, PCR amplification is carried out using universal primer 8F/1492R.After PCR product electrophoresis detection, 16S rDNA gene order, tool are measured
Body sequence is as shown in sequence 1 in sequence table.Sequence 1 is found into W-Y10 bacterial strain category by blast search in NCBI and after comparing
In Aquamicrobium bacterium, but the similitude with having bacterial strain is lower.Use software MEGA phylogenetic tree construction such as Fig. 1 institute
Show.By similitude, in 97% or more bacterial strain, to reuse software MEGA phylogenetic tree construction as shown in Figure 2.Wherein, W-Y10
The physiological and biochemical property comparison result of bacterial strain and the highest Aquamicrobium aestuarii bacterial strain of similitude is as shown in table 1,
Illustrate that W-Y10 bacterial strain is one plant of new strains.
The physiological and biochemical property comparison result of table 1, W-Y10 bacterial strain and Aquamicrobium aestuarii bacterial strain
| Number | Colony colour | K-IAO | PEARLITOL 25C |
| W-Y10 | Khaki | - | - |
| Aquamicrobium aestuarii | Ivory white | + | + |
Note :-indicate not grow ,+indicate growth
In summary qualification result shows that bacterial strain W-Y10 is a kind of newfound Aquamicrobium bacterium.
Three, the preservation of W-Y10 bacterial strain
The classification naming of W-Y10 bacterial strain is Aquamicrobium sp., which was preserved on September 26th, 2018
China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address: Chaoyang District, Beijing City North Star west
The institute 3 of road 1, Institute of Microorganism, Academia Sinica, postcode 100101), deposit number is CGMCC No.16533.
Embodiment 2, W-Y10 bacterial strain are to emulsified viscous oil
Petroleum in the present embodiment acquires the North China Oilfield from Inner Mongol Erlianhaote, 35 DEG C of petroleum in May, 2017
Viscosity is 570mPa.s.
1, the W-Y10 after activation is seeded in LB liquid medium, 35 DEG C, 150rpm shaken cultivation for 24 hours, obtain W-
Y10 bacterium solution (bacteria concentration >=108cfu/mL)。
2, test group: by 5 parts by volume bacterium solutions be seeded to 95 parts by volume petroleum-minimal medium (solvent is water, solute and
Its concentration is respectively 1g/L petroleum, 5g/L NaCl, 1g/L NH4H2PO4、1g/L(NH4)2SO4、1g/L K2HPO4、3g/L
KNO3, pH 7-8) in, 35 DEG C, 150rpm shaken cultivation 7d.
Control group: by 100 parts by volume petroleum-minimal medium, 35 DEG C, 150rpm shaken cultivation 7d.
3, after completing step 2, photographic result is shown in Fig. 3.In Fig. 3, the left side is experimental group, and the right is control group.Bacterial strain W-Y10
There is apparent emulsification to viscous crude at 35 DEG C.
4, it is completed in the system of step 2 according to the method measurement in document " separation of oil degradation bacterium and its degradation property "
Petroleum residual amount, calculate petroleum degradation rate.Petroleum degradation rate=(petroleum additional amount-petroleum residual amount)/petroleum additional amount.Meter
Calculate the result shows that: petroleum degradation rate 50.1%.
Embodiment 3, the application of W-Y10 bacterial strain in microbial oil extraction technology
Petroleum in the present embodiment acquires the North China Oilfield from Inner Mongol Erlianhaote, 35 DEG C of petroleum in May, 2017
Viscosity is 570mPa.s.
The present embodiment passes through the oil displacement efficiency that indoor object mould oil displacement experiment evaluates microorganism fungus kind.Specific experiment method is such as
Under:
1, using High Pressure Model pipe (specificationPurchase is to Hai'an petroleum scientific research Instrument Ltd.) in load
The quartz sand of different meshes is made as sandpack column pipe.Using permeability measuring apparatus measurement model pipe permeability, selection meets
Model pipe.Saturation water flooding is vacuumized, porosity is calculated；The parameter of the model pipe for subsequent step finally prepared is shown in Table
2。
Table 2, model pipe back-up sand parameter
2, petroleum is persistently injected from model tube inlet, until model pipe outlet detects that petroleum flows out, measures initial oil
Saturation degree；And aging 3 days under reservoir temperature (35 DEG C).
3, the displacement of reservoir oil is carried out according to the injection rate injection water of 1 model pipe pore volume volume, until outlet production fluid reaches pole
It limits moisture content (98%) and stops water drive, calculate recovery ratio (as waterflood recovery efficiency factor).
4, according to the injection rate injection infected water of 1 model pipe pore volume volume (after completing injection, calculate recovery ratio,
As infuse microbial process recovery ratio), it is cultivated 15 days under reservoir temperature (35 DEG C).
Infected water the preparation method is as follows: the W-Y10 of activation is seeded in LB liquid medium, 35 DEG C, 150rpm vibration
It swings culture for 24 hours, obtains W-Y10 bacterium solution (bacteria concentration >=108cfu/mL).W-Y10 bacterium solution, NH is added in injection water4H2PO4、
(NH4)2SO4、K2HPO4、KNO3, peptone and yeast powder, make it in the middle concentration of infected water be respectively 5% (volume fraction) W-
Y10 bacterium solution, 1g/L NH4H2PO4、1g/L(NH4)2SO4、1g/L K2HPO4、3g/L KNO3, 2g/L peptone and 1g/L yeast leaching
Powder.
5, the displacement of reservoir oil is carried out according to the injection rate injection water of 1 model pipe pore volume volume, until outlet production fluid reaches pole
It limits moisture content (98%) and stops water drive, calculate recovery ratio (as microbial action subsequent waterflooding recovery ratio).Object mould displacement of reservoir oil result
Curve is as shown in Figure 4.
To in water drive, note microbial process, the recovery ratio of the different phases such as microbial action subsequent waterflooding compared,
It the results are shown in Table 3.The indoor object mould displacement of reservoir oil shows that recovery ratio 8.38% can be improved using W-Y10 bacterial strain.
Table 3, recovery ratio
| Object mode step section | Recovery ratio (%) | It improves recovery ratio (%) |
| Water drive | 48.24 | / |
| Infuse microbial process | 52.65 | 4.41 |
| Microbial action subsequent waterflooding | 56.62 | 8.38 |
Sequence table
<110>Beijing Run Shi Enertech Co., Ltd.
<120>one plants of microbe oil production bacterium W-Y10 and its applications
<160>1
<170>PatentIn version 3.5
<210>1
<211>1334
<212>DNA
<213>artificial sequence (Artificial Sequence)
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ctacggaata acccagggaa acttggacta ataccgtata cgtccttcgg gagaaagatt 120
tatcggagtt ggatgagccc gcgtcggatt agctagttgg tgaggtaatg gctcaccaag 180
gcgacgatcc gtagctggtc tgagaggatg atcagccaca ctgggactga gacacggccc 240
agactcctac gggaggcagc agtggggaat attggacaat gggcgaaagc ctgatccagc 300
catgccgcgt gagtgatgaa ggccctaggg ttgtaaagct ctttcaccgg ggaagataat 360
gacggtaccc ggagaagaag ccccggctaa cttcgtgcca gcagccgcgg taatacgaag 420
ggggctagcg ttgttcggaa ttactgggcg taaagcgcac gtaggcggac tattaagtca 480
ggggtgaaat cccggggctc aaccccggaa ctgcctttga tactggtagt ctagagttcg 540
agagaggtga gtggaattcc gagtgtagag gtgaaattcg tagatattcg gaggaacacc 600
agtggcgaag gcggctcact ggctcgatac tgacgctgag gtgcgaaagc gtggggagca 660
aacaggatta gataccctgg tagtccacgc cgtaaacgat ggaagctagc cgttggcaag 720
tttacttgtc ggtggcgcag ctaacgcatt aagcttcccg cctggggagt acggtcgcaa 780
gattaaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt 840
cgaagcaacg cgcagaacct taccagccct tgacatcccg atcgcggtta ccggagacgg 900
tttccttcag ttcggctgga tcggtgacag gtgctgcatg gctgtcgtca gctcgtgtcg 960
tgagatgttg ggttaagtcc cgcaacgagc gcaaccctcg cccttagttg cccttattaa 1020
gttaggcact ctaaggggac tgccggtgat aagccgagag gaaggtgggg atgacgtcaa 1080
gtcctcatgg cccttacggg ctgggctaca cacgtgctac aatggtggtg acagtgggca 1140
gcgagaccgc gaggtcgagc taatctccaa aagccatctc agttcggatt gcactctgca 1200
actcgagtgc atgaagttgg aatcgctagt aatcgcggat cagcatgccg cggtgaatac 1260
gttcccgggc cttgtacaca ccgcccgtca caccatggga gttggcttta cccgaaggcg 1320
ctgcgctaac cgca 1334
Claims (10)
1. one plant of Aquamicrobium sp.W-Y10, deposit number is CGMCC No.16533.
2. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in petroleum recovering；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing petroleum recovering.
3. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in microbe oil production；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing microbe oil production.
4. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in emulsification of crude oil；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing emulsification of crude oil.
5. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in oil degradation；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing oil degradation.
6. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Application containing its microbial inoculum in petroleum pollution；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or the application containing its microbial inoculum in the product for preparing petroleum pollution.
7. Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning or
Containing its microbial inoculum oil pollution reparation in application；
Or, Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or its culture solution or its tunning
Or containing its microbial inoculum preparation oil pollution reparation product in application.
8. a kind of product, active constituent be Aquamicrobium sp.W-Y10 described in claim 1 or its bacteria suspension or
Its culture solution or its tunning or the microbial inoculum containing it；
The product has following 1) -6) in any function:
1) petroleum recovering；
2) microbe oil production；
3) emulsification of crude oil；
4) oil degradation；
5) petroleum pollution；
6) oil pollution reparation.
9. a kind of method of microbe oil production, includes the following steps: during oil recovery, it is added described in claim 1
Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or its tunning or the microbial inoculum containing it.
10. a kind of petroleum pollution and/or the method for reparation, include the following steps: with described in claim 1
Aquamicrobium sp.W-Y10 or its bacteria suspension or its culture solution or its tunning or containing its microbial inoculum handle petroleum
Pollutant.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961614A (en) * | 2020-08-04 | 2020-11-20 | 安徽科技学院 | Novel bacterial strain cd-1 for producing exopolysaccharides |
| CN115725432A (en) * | 2021-08-31 | 2023-03-03 | 中国石油天然气股份有限公司 | Acinetobacter capable of degrading heavy components of crude oil to produce surfactant and application of acinetobacter |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016122333A1 (en) * | 2015-01-28 | 2016-08-04 | Statoil Petroleum As | An automated method for selecting microbial strains which can degrade or emulsify oil |
| CN106635908A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent |
| KR101874956B1 (en) * | 2017-01-20 | 2018-07-05 | 신명옥 | Pseudomonas putida MZ3 bacteria for purifying oil-contaminated soil |
| CN108587987A (en) * | 2018-07-23 | 2018-09-28 | 北京润世能源技术有限公司 | One plant of microbe oil production bacterium W-Y6 and its application |
-
2018
- 2018-10-25 CN CN201811252377.0A patent/CN109234201B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016122333A1 (en) * | 2015-01-28 | 2016-08-04 | Statoil Petroleum As | An automated method for selecting microbial strains which can degrade or emulsify oil |
| CN106635908A (en) * | 2016-12-29 | 2017-05-10 | 长安大学 | Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent |
| KR101874956B1 (en) * | 2017-01-20 | 2018-07-05 | 신명옥 | Pseudomonas putida MZ3 bacteria for purifying oil-contaminated soil |
| CN108587987A (en) * | 2018-07-23 | 2018-09-28 | 北京润世能源技术有限公司 | One plant of microbe oil production bacterium W-Y6 and its application |
Non-Patent Citations (3)
| Title |
|---|
| MADDELA NAGA RAJU等: "Petroleum Deradation： Promising Biotechnological Tools for Bioremediation", 《RECENT INSIGHTS IN PETROLEUM SCIENCE AND ENGINEERING》 * |
| XINXIN WANG等: "Draft Genome Sequence of Aquamicrobium defluvii Strain W13Z1，a Psychrotolerant Halotolerant Hydrocarbon Degrading Bacterium", 《GENOME ANNOUNCEMENTS》 * |
| 郑宁来: "中科院发现微生物采油菌株", 《炼油技术与工程》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961614A (en) * | 2020-08-04 | 2020-11-20 | 安徽科技学院 | Novel bacterial strain cd-1 for producing exopolysaccharides |
| CN111961614B (en) * | 2020-08-04 | 2022-04-29 | 安徽科技学院 | Bacterium cd-1 for producing exopolysaccharide |
| CN115725432A (en) * | 2021-08-31 | 2023-03-03 | 中国石油天然气股份有限公司 | Acinetobacter capable of degrading heavy components of crude oil to produce surfactant and application of acinetobacter |
| CN115725432B (en) * | 2021-08-31 | 2023-11-28 | 中国石油天然气股份有限公司 | Acinetobacter capable of degrading crude oil heavy component to produce surfactant and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109234201B (en) | 2020-07-28 |
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