CN102391847A - Composite microbe oil displacement agent and use thereof - Google Patents
Composite microbe oil displacement agent and use thereof Download PDFInfo
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- CN102391847A CN102391847A CN2011102931610A CN201110293161A CN102391847A CN 102391847 A CN102391847 A CN 102391847A CN 2011102931610 A CN2011102931610 A CN 2011102931610A CN 201110293161 A CN201110293161 A CN 201110293161A CN 102391847 A CN102391847 A CN 102391847A
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Abstract
The invention discloses a composite microbe oil displacement agent and the use thereof. The composite microbe oil displacement agent comprises pseudomonas fermentation anaculture and geobacillus fermentation anaculture, and the volume ratio of the pseudomonas fermentation anaculture to the geobacillus fermentation anaculture ranges from 1:10 to 10:1. The composite microbe oil displacement agent is non-toxic and harmless, is easy to degrade, and is an environmental-friendly biological agent; the manufacturing cost is low, and the composite microbe oil displacement agent is easy to manufacture and apply on a large scale; the using temperature range is wide (from 10 to 100 DEG C), and high-viscosity crude oil emulsion can be processed through emulsion breaking effectively; deposits cannot be generated after the composite microbe oil displacement agent is mixed with high hardness groundwater, and the composite microbe oil displacement agent is suitable for oil displacement, plugging removal and emulsion breaking of a low permeability oilfield and an ultra-low permeability oilfield; and the composite microbe oil displacement agent achieves high efficiency of oil displacement and emulsion breaking.
Description
Technical field
The present invention relates to a kind of composite microorganism oil-displacing agent, be specifically related to contain Pseudomonas aeruginosa ferment full culture and thermophilic denitrifying ground bacillus the ferment composite microorganism oil-displacing agent and the application in the oil field displacement of reservoir oil, de-plugging of well and oil-field water oil-in extraction liquid and oil-in-water emulsion breakdown of emulsion thereof of full culture.
Background technology
Improving oil recovery is the key issue in the petroleum industry.Although the purpose that physics that uses at present and chemical means all can reach effective displacement of reservoir oil, high energy consumption high ambient are polluted the bottleneck that but becomes the development of restriction petroleum industry.In recent years, both at home and abroad to using fields such as microorganism oil-displacing agent displacement of reservoir oil, de-plugging, breakdown of emulsion and environment protection to carry out a large amount of research in the oil field, yet because of factors such as production cost is high, oil displacement efficiency is not good not seen widespread use still.
Summary of the invention
The object of the present invention is to provide that a kind of oil-displacement capacity is strong, production cost is lower, nontoxic, it is not quick-fried not fire, degradable, pollution-free to oil reservoir, construction safety reliable, be easy to the composite microorganism oil-displacing agent of scale operation and non-environmental-pollution; This composite microorganism oil-displacing agent reaches displacement of reservoir oil purpose through metabolism products such as the glycolipid that produces in the fermentation process and lipopeptids, and can be simultaneously through mikrobe on the high infiltration of oil reservoir ground carrying propagation realize the purpose of profile adjustment and oil displacement.
For achieving the above object, implementation procedure of the present invention is following:
The bacterial classification that is used to prepare composite microorganism oil-displacing agent disclosed by the invention is pseudomonas and ground bacillus, and wherein pseudomonas is preferably Pseudomonas aeruginosa, and ground bacillus is preferably the thermophilic denitrifying ground bacillus.
Pseudomonas aeruginosa and thermophilic denitrifying ground bacillus all derive from American type culture collection, and preserving number is respectively ATCC9027 and ATCC29492.
At first being to produce bacterial strain with the Pseudomonas aeruginosa, is that main carbon source is fermented with vegetables oil, obtains the false unit cell of the verdigris full culture that ferments; Serving as to produce bacterial strain with the thermophilic denitrifying ground bacillus then, is that main carbon source is fermented with sucrose and starch, the acquisition thermophilic denitrifying ground bacillus full culture that ferments; At last ferment full culture and thermophilic denitrifying ground bacillus of Pseudomonas aeruginosa fermented and promptly get composite microorganism oil-displacing agent after full culture mixes with certain proportion, concrete steps are:
Cultivation of Pseudomonas aeruginosa and fermentation: with the Pseudomonas aeruginosa is bacterial strain, and the selection vegetables oil is a carbon source, obtains efficient haemolysis bacterial strain through blood agar is streak culture with shake-flask culture; In seed culture fluid, 30 ℃, aerobic culture 12～24h obtains seed liquor with bacterial classification inoculation; Seed liquor is inoculated in the Pseudomonas aeruginosa fermention medium of 10 times of volumes the full culture of aerobic culture 24～144h results fermentation.
The cultivation of thermophilic denitrifying ground bacillus and fermentation: with the thermophilic denitrifying ground bacillus is bacterial strain, and selecting sucrose and starch is carbon source, obtains efficient haemolysis bacterial strain through blood agar is streak culture with shake-flask culture.In seed culture fluid, 60 ℃, aerobic culture 12～24h obtains seed liquor with bacterial classification inoculation; Seed liquor is inoculated in the fermention medium of 10 times of volumes the full culture of static cultivation 24～72h results fermentation.
Seed culture medium: contain fish meal 0～30.0g/L, yeast extract 1～20.0g/L and sodium-chlor 5～20.0g/L.
Pseudomonas aeruginosa fermention medium: contain urea 0.1～2.0g/L, K
2HPO
40.1～5.0g/L, MgSO
4.6H
2O 1.0～5.0mg/L, FeCl
3.6H
2O 0.01～0.50 mg/L, ZnSO
4.7H
2O 1.0～5.0 mg/L, CuSO
4.5H
2O 0.05～0.30mg/L, MnSO
4.4H
2O 0.5～5.0mg/L, H
3BO
30.01～0.05mg/L, fish meal 3.0～12.0g/L, vegetables oil 1～100mL/L.
Thermophilic denitrifying ground bacillus fermention medium: contain sucrose 1～20.0g/L, starch 1～10.0 g/L, NH
4Cl 0.1～10.0g/L, MgSO
4.7H
2O 0.1～0.5g/L, KH
2PO
41.0～10.0g/L, Na
2HPO
41.0～20.0g/L, EDTA 0.01～0.30g/L, CaCl
20.01～0.20g/L, fish meal 3.0～12.0g/L.
The culture temperature that Pseudomonas aeruginosa of the present invention is fit to is 20～40 ℃, and the pH value that is fit to is 6.0～8.0; The culture temperature that the thermophilic denitrifying ground bacillus is fit to is 50～65 ℃, and the pH value that is fit to is 6.0～8.0.
The configuration of composite microorganism oil-displacing agent: Pseudomonas aeruginosa full culture and the thermophilic denitrifying ground bacillus full culture that ferments that ferments is mixed with volume ratio 1:10～10:1 and promptly gets the compound bio oil-displacing agent.
The storage temperature of composite microorganism oil-displacing agent: 4～50 ℃.
It is better that composite microorganism oil-displacing agent of the present invention and chemical surfactant carry out composite back result of use, and said chemical surfactant is sulfonated petro-leum, OP-10,8390 etc.
Composite microorganism oil-displacing agent of the present invention can be used for the oil field displacement of reservoir oil or oil-field water oil-in extraction liquid or oil-in-water emulsion breakdown of emulsion to be handled.When the displacement of reservoir oil, with 1:1～1:200 dilution, utilizing the oil-field flooding station to inject behind the oilbearing stratum can effective displacement of reservoir oil, reaches the purpose that improves RF with composite microorganism oil-displacing agent and underground water.Also can the full culture of microbial fermentation be mixed the back with crude oil and realize breakdown of emulsion, utilize oil field and the existing dosing of other industries, stirring, sedimentation and oily water separation facility can realize effective breakdown of emulsion oil-in-water-type extraction liquid and other emulsion.
Composite microorganism oil-displacing agent of the present invention has the following advantages:
1) nontoxic, easy degraded is the environment friendly biotechnological formulation;
2) low production cost is easy to scale operation and application;
3) use temperature scope big (10～100 ℃) can be carried out effective breakdown of emulsion to the high viscosity oil emulsion;
4) with after high firmness underground water mixes do not produce deposition, be applicable to displacement of reservoir oil de-plugging and breakdown of emulsion hypotonic, special low-permeability oilfield;
5) displacement of reservoir oil breakdown of emulsion efficient is high;
6) interpolation concentration is low, and the maximum dilution coefficient is 1:500 during the displacement of reservoir oil, and the minimum quantity adding is merely emulsion volume 0.5% during breakdown of emulsion.
Embodiment
Below through embodiment the present invention is described further, the present invention does not receive the restriction of following concrete text description.The present invention can make various changes in the generalized scope of claim institute, these change all within protection domain of the present invention.Unreceipted actual conditions person among the embodiment is undertaken by the condition of routine or manufacturers's suggestion.
The ferment preparation of full culture of embodiment 1 Pseudomonas aeruginosa
Frozen Pseudomonas aeruginosa streak inoculation on the LB flat board that contains 10g/L Tryptones, 5g/L yeast extract, 10g/L NaCl and 15g/L agar, is placed incubated overnight under 37 ℃, saturated humidity condition.The dull and stereotyped bacterium colony of cultivating of picking LB is inoculated in the LB substratum that contains 10g/L Tryptones, 5g/L yeast extract and 10g/L NaCl 37 ℃, 220rpm incubated overnight.Overnight culture is inoculated in the 1:10 ratio in the LB substratum that contains the 5.0g/L fish meal, cultivates 12h under 37 ℃, air flow 1.0VVM condition, obtain the Pseudomonas aeruginosa seed liquor.Seed liquor is inoculated in the 1:10 ratio contains urea 0.5g/L, K
2HPO
42.0g/L, MgSO
4.6H
2O 5.0mg/L, FeCl
3.6H
2O 0.16mg/L, ZnSO
4.7H
2O 1.5mg/L, CuSO
4.5H
2O 0.15mg/L, MnSO
4.4H
2O 1.5mg/L, H
3BO
30.03mg/L vegetables oil 80mL/L in the fermentation culture of fish meal 5.0g/L, at 30 ℃, air flow 1.0VVM condition bottom fermentation 72h, obtains the Pseudomonas aeruginosa full culture that ferments, room temperature preservation is subsequent use after the packing.
Embodiment 2 Pseudomonas aeruginosas the composite of full culture and chemical surfactant of fermenting
In embodiment 1 Pseudomonas aeruginosa is fermented full culture, adding nonionogenic tenside OP-10 is 6% to final concentration, and room temperature preservation is subsequent use after the packing.
The ferment preparation of full culture of embodiment 3 ground bacillus
Frozen thermophilic denitrifying ground bacillus streak inoculation on the LB flat board that contains 10g/L Tryptones, 5g/L yeast extract, 10g/L NaCl and 15g/L agar, is placed incubated overnight under 60 ℃, saturated humidity condition.The dull and stereotyped thermophilic denitrifying ground bacillus of cultivating of picking LB is inoculated in the LB substratum that contains 10g/L Tryptones, 5g/L yeast extract and 10g/L NaCl 60 ℃, 220rpm incubated overnight.Thermophilic denitrifying ground bacillus overnight culture is inoculated in the LB substratum with the 1:10 ratio, cultivates 12h under 60 ℃, air flow 1.0VVM condition, obtain thermophilic denitrifying ground bacillus seed liquor.Thermophilic denitrifying ground bacillus seed liquor is inoculated in the 1:10 ratio contains sucrose 10.0g/L, starch 10.0g/L, NH
4Cl 6.0g/L, MgSO
4.7H
2O 0.2g/L, KH
2PO
44.0g/L, Na
2HPO
412.0g/L, EDTA 0.14g/L, CaCl
20.08g/L, in the fermention medium of fish meal 3.0g/L,, obtaining the thermophilic denitrifying ground bacillus full culture that ferments at 60 ℃ of static fermentation 48h, room temperature preservation is subsequent use after the packing.
Embodiment 4 thermophilic denitrifying ground bacillus the composite of full culture and chemical surfactant of fermenting
In the thermophilic denitrifying ground bacillus ferments full culture, adding AS 8390 to final concentration is 10%, and room temperature preservation is subsequent use after the packing.
The configuration of embodiment 5 composite microorganism oil-displacing agents
With the Pseudomonas aeruginosa of embodiment 1 ferment the thermophilic denitrifying ground bacillus of full culture and embodiment 3 ferment full culture with volume ratio 3:7 mixed after packing promptly get composite microorganism oil-displacing agent, room temperature preservation is subsequent use after the packing.
The configuration of embodiment 6 composite microorganism oil-displacing agents
The Pseudomonas aeruginosa that embodiment 2 is contained tensio-active agent ferment thermophilic denitrifying ground bacillus that full culture and embodiment 4 contain tensio-active agent ferment full culture with volume ratio 7:3 mixed after packing promptly get composite microorganism oil-displacing agent, room temperature preservation is subsequent use after the packing.
Embodiment 7 composite microorganism oil-displacing agents and chemical surfactant composite
With the Pseudomonas aeruginosa of embodiment 1 ferment the thermophilic denitrifying ground bacillus of full culture and embodiment 3 ferment full culture with volume ratio 5:5 mixed after packing promptly get composite microorganism oil-displacing agent; In composite microorganism oil-displacing agent, add nonionogenic tenside OP-10 to final concentration be 5%; Perhaps adding AS 8390 to final concentration is 10%, and room temperature preservation is subsequent use after the packing.
The indoor core oil displacement experiment of embodiment 8 composite microorganism oil-displacing agents
Use embodiment 5 composite microorganism oil-displacing agents to carry out oil displacement experiment at natural core, the result shows that complex microorganism drives that on average to improve RF than water drive about 24.6%, displaces the liquid water cut and significantly descends.The different artificial homogeneous core of use rate of permeation carries out and unites to annotate to divide and adopt oil displacement experiment; The result shows: when water drive finishes; High, in, the pervious course production fluid amount ratio that accounts for total liquid measure be respectively 75.7%, 23.4% and 0.9%; Inject liquid when showing water drive and mainly flow, and most permeable zone water drive recovery percent of reserves is high, is about 62.9% at most permeable zone.Middle low permeability layer recovery percent of reserves then is merely 32.6% and 4.9%, does not employ Chuck Steak.
Use separately embodiment 1 Pseudomonas aeruginosa to ferment the full culture displacement of reservoir oil when finishing, high, in, the pervious course production fluid amount ratio that accounts for total liquid measure be respectively 82.3%, 16.7% and 0.7%, recovery percent of reserves is respectively 68.2%, 15.5% and 3.2%.Be illustrated in when using Pseudomonas aeruginosa to ferment the full culture displacement of reservoir oil, inject liquid and mainly flow, and increase RF through improving most permeable zone crude oil recovery percent of reserves at most permeable zone.
Use separately embodiment 3 thermophilic denitrifying ground bacillus to ferment the full culture displacement of reservoir oil when finishing, high, in, the pervious course production fluid amount ratio that accounts for total liquid measure be respectively 63.5%, 32.7% and 3.8%, the crude oil recovery percent of reserves is respectively 58.7%, 32.6% and 9.3%.This result show the thermophilic denitrifying ground bacillus ferment full culture can be obviously through improve interlayer contradiction and improve in low permeability layer crude oil recovery percent of reserves.
When the complex microorganism displacement of reservoir oil finishes, high, in, the pervious course production fluid amount ratio that accounts for total liquid measure be respectively 58.1%, 32.5% and 9.4%, the crude oil recovery percent of reserves is respectively 69.1%, 37.1% and 17.8%, shows that complex microorganism drives can significantly improve interlayer contradiction.Place 60 ℃ of simulated formation environment to carry out water drive after 48 hours the parallelly connected core behind the microbial flooding; High, in, the pervious course production fluid amount ratio that accounts for total liquid measure be respectively 38.9%, 41.2% and 19.9%; Be illustrated under the simulation stratum condition complex microorganism and drive and more effectively to improve interlayer contradiction, thereby improve oil recovery factor through not employing oil.
In sum, can significantly increase crude oil fluidity, improve different rate of permeation ground interlayer contradiction by Pseudomonas aeruginosa full culture and the thermophilic denitrifying ground bacillus composite microorganism oil-displacing agent that full culture compatibility (embodiment 5-7) forms that ferments that ferments.With independent use Pseudomonas aeruginosa ferment full culture with use separately the thermophilic denitrifying ground bacillus full culture that ferments to compare; Composite microorganism oil-displacing agent provided by the invention can more effectively improve oil recovery factor, the crude oil recovery percent of reserves of low permeability layer in especially can significantly improving.
Claims (7)
1. composite microorganism oil-displacing agent is characterized in that comprising: pseudomonas full culture and the ground bacillus full culture that ferments that ferments, two kinds of bacterium are fermented full culture volume than being 1:10 ~ 10:1.
2. composite microorganism oil-displacing agent according to claim 1 is characterized in that: the pseudomonas full culture that ferments is containing urea 0.1～2.0g/L, K by Pseudomonas aeruginosa
2HPO
40.1～5.0g/L, MgSO
4.6H
2O 1.0～5.0mg/L, FeCl
3.6H
2O 0.01～0.50 mg/L, ZnSO
4.7H
2O 1.0～5.0 mg/L, CuSO
4.5H
2O 0.05～0.30mg/L, MnSO
4.4H
2O 0.5～5.0mg/L, H
3BO
30.01～0.05mg/L, fish meal 3.0～12.0g/L, with 30～40 ℃, air flow 0.5～5.0VVM condition fermentation 24～144h obtains in the substratum of vegetables oil 1～100mL/L.
3. composite microorganism oil-displacing agent according to claim 2 is characterized in that: the pseudomonas culture temperature that full culture is fit to of fermenting is 20～40 ℃, and the pH value that is fit to is 6.0～8.0.
4. composite microorganism oil-displacing agent according to claim 1 is characterized in that: the ground bacillus full culture that ferments is containing sucrose 1～20.0g/L by the thermophilic denitrifying ground bacillus, starch 1～10.0 g/L, NH
4Cl 0.1～10.0g/L, MgSO
4.7H
2O 0.1～0.5g/L, KH
2PO
41.0～10.0g/L, Na
2HPO
41.0～20.0g/L, EDTA 0.01～0.30g/L, CaCl
20.01～0.20g/L obtains with 50～65 ℃ of static fermentations or low ventilation fermentation 24～72h in the substratum of fish meal 3.0～12.0g/L.
5. composite microorganism oil-displacing agent according to claim 4 is characterized in that: the ground bacillus culture temperature that full culture is fit to of fermenting is 50～65 ℃, and the pH value that is fit to is 6.0～8.0.
6. composite microorganism oil-displacing agent according to claim 1 is characterized in that: be added with nonionogenic tenside OP-10 and/or AS 8390.
7. the application of the described composite microorganism oil-displacing agent of claim 1 in the oil field displacement of reservoir oil, de-plugging of well, oil-field water oil-in extraction liquid and oil-in-water emulsion breakdown of emulsion.
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| CN102852497A (en) * | 2012-08-07 | 2013-01-02 | 陕西延长石油(集团)有限责任公司研究院 | Compound microorganism oil extraction method for low permeability oilfield |
| CN103614131A (en) * | 2013-12-12 | 2014-03-05 | 大连奥普森生物工程有限公司 | Biochemical anti-salt anti-high temperature oil displacement viscosity reducer and preparation method thereof |
| CN104109516A (en) * | 2014-06-23 | 2014-10-22 | 中国石油化工股份有限公司 | Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof |
| CN104263343A (en) * | 2014-09-09 | 2015-01-07 | 中国石油化工股份有限公司 | Compound microorganism oil-displacing agent for oil production and compounding method thereof |
| CN104893700A (en) * | 2015-03-30 | 2015-09-09 | 北京世纪金道石油技术开发有限公司 | Composite microbial agent, preparation method therefor and application thereof |
| CN105694952A (en) * | 2016-03-15 | 2016-06-22 | 常州市蓝勖化工有限公司 | Preparation method of compound microorganism demulsifying agent |
| CN108559720A (en) * | 2018-04-26 | 2018-09-21 | 长江大学 | One kind is for oil well paraffin prevention corrosion-resistant microbial bacterial agent and preparation method thereof |
| CN110951472A (en) * | 2019-12-09 | 2020-04-03 | 李国斌 | Biochemical combined-effect blocking remover and preparation method thereof |
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| CN114507516A (en) * | 2022-04-18 | 2022-05-17 | 胜利油田新海兴达实业集团有限责任公司 | Biological enzyme composite blocking remover and preparation method thereof |
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| CN102660486A (en) * | 2012-01-21 | 2012-09-12 | 大地绿源环保科技(北京)有限公司 | Geobacillus sp. UTM01 and application thereof |
| CN102852497A (en) * | 2012-08-07 | 2013-01-02 | 陕西延长石油(集团)有限责任公司研究院 | Compound microorganism oil extraction method for low permeability oilfield |
| CN102852497B (en) * | 2012-08-07 | 2015-09-30 | 陕西延长石油(集团)有限责任公司研究院 | A kind of compound microorganism oil extraction method for low permeability oilfield |
| CN103614131A (en) * | 2013-12-12 | 2014-03-05 | 大连奥普森生物工程有限公司 | Biochemical anti-salt anti-high temperature oil displacement viscosity reducer and preparation method thereof |
| CN103614131B (en) * | 2013-12-12 | 2016-01-13 | 大连奥普森生物工程有限公司 | A kind of Biochemical anti-salt anti-high temperature oil displacement viscosity reducer and preparation method thereof |
| CN104109516B (en) * | 2014-06-23 | 2017-01-11 | 中国石油化工股份有限公司 | Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof |
| CN104109516A (en) * | 2014-06-23 | 2014-10-22 | 中国石油化工股份有限公司 | Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof |
| CN104263343A (en) * | 2014-09-09 | 2015-01-07 | 中国石油化工股份有限公司 | Compound microorganism oil-displacing agent for oil production and compounding method thereof |
| CN104893700A (en) * | 2015-03-30 | 2015-09-09 | 北京世纪金道石油技术开发有限公司 | Composite microbial agent, preparation method therefor and application thereof |
| CN105694952A (en) * | 2016-03-15 | 2016-06-22 | 常州市蓝勖化工有限公司 | Preparation method of compound microorganism demulsifying agent |
| CN108559720A (en) * | 2018-04-26 | 2018-09-21 | 长江大学 | One kind is for oil well paraffin prevention corrosion-resistant microbial bacterial agent and preparation method thereof |
| CN111022006A (en) * | 2019-12-06 | 2020-04-17 | 大庆金军石油科技开发有限公司 | Method for improving shale oil well recovery ratio through carbon dioxide composite huff and puff |
| CN110951472A (en) * | 2019-12-09 | 2020-04-03 | 李国斌 | Biochemical combined-effect blocking remover and preparation method thereof |
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| CN114507516A (en) * | 2022-04-18 | 2022-05-17 | 胜利油田新海兴达实业集团有限责任公司 | Biological enzyme composite blocking remover and preparation method thereof |
| CN114507516B (en) * | 2022-04-18 | 2022-07-05 | 胜利油田新海兴达实业集团有限责任公司 | Biological enzyme composite blocking remover and preparation method thereof |
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Application publication date: 20120328 |