CN101493003B - Microbe oil production method after polymer drive - Google Patents
Microbe oil production method after polymer drive Download PDFInfo
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- CN101493003B CN101493003B CN2008102391112A CN200810239111A CN101493003B CN 101493003 B CN101493003 B CN 101493003B CN 2008102391112 A CN2008102391112 A CN 2008102391112A CN 200810239111 A CN200810239111 A CN 200810239111A CN 101493003 B CN101493003 B CN 101493003B
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- General Life Sciences & Earth Sciences (AREA)
- Fluid Mechanics (AREA)
- Physics & Mathematics (AREA)
- Geochemistry & Mineralogy (AREA)
- Environmental & Geological Engineering (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a microbial oil production method, including a process of displacement of reservoir oil by using bacterial liquid after polymer flooding of oil layers; the bacterial liquid is a mixed liquid or zymotic fluid of bacillus fusiformis 6# CGMCC No.2439, Potsdam Bacillus brevis Po CGMCC No.2441 or Bacillus licheniformis U1-3 CGMCC No.2437 singly mixed with a culture medium, or compatible liquid of the three with the culture medium. The experimental results of model displacement of reservoir oil show that the bacterial liquid is used for displacement of reservoir oil after the polymer flooding, so that recovery ratio is increased by 3-5 percent (OOIP), the bacterial liquid, coupled with polymer protection slug flooding, is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio is increased by about 7-9 percent (OOIP), and a method with the bacterial liquid displacement and chemical displacement combined is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio can be increased by 13% (OOIP). The experimental results show that the method of the invention can be widely applied to the field of oil production engineering, especially the field of microbial intensified oil production, and is suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to the microbe oil production field, be specifically related to utilize the method for the remaining oil behind the microorganism extraction polymer flooding.
Background technology
Oil is a kind of non-renewable energy resources, and in order to effectively utilize petroleum resources, the high efficiency method of recovery ratio is being sought to improve in countries in the world, is stranded in the stratum to exploit those, the crude oil that is difficult to exploit with conventional oil production method.From the eighties in last century, a lot of countries have carried out with microbial process improves the oil recovery factor Study on Technology, and through the effort of two more than ten years, this technology has obtained very big progress.
The Daqing oil field polymer flooding is through country " eight or five ", " 95 " emphasis tackling of key scientific and technical problems; Obtained breakthrough progress; Enter into the industrialness application stage by pilot field test, recovery ratio improves about 10% than water drive, and annual production reaches more than 1,000 ten thousand tons.By the end of the year 2007,43 of polymer industry blocks are employed area and are reached 365.97Km
2, employ 5.95 hundred million tons of oils in place, become the great technical measures that Daqing oil field prolongs stable production period.However, still have nearly half the crude oil not by extraction behind the polymer flooding in the oil reservoir.
For addressing the above problem; The researcher has proposed to utilize behind the polymer flooding microorganism to combine the exploratory development that further improves recovery ratio with chemical flooding; So that the remaining oil behind the extraction polymer flooding to greatest extent, further increase the recoverable reserves in oil field, improve the ultimate recovery in oil field.As everyone knows, when polymer process reservoir, a part is adsorbed and is trapped on blowhole and the surface, and according to document announcement, the polymer of every cubic feet of reservoir delay reaches the 22.7-45.4 kilogram.
The microorganism of polymer capable of using is injected the oil reservoir that contains polymer, and microorganism is at underground metabolic exhaustion polymer, and in the microbiologic population of an external source of underground formation.This method has two advantages, and the one, the microorganism decomposable polymer is removed the polymer that stops up oil reservoir effectively; The 2nd, the microorganism of injecting can produce material such as organic acid, the active material etc. that help the displacement of reservoir oil in the process of utilizing polymer growth and metabolism, improves acid value for crude oil, discharges crude oil, increases output, finally reaches the purpose that improves recovery ratio.
Through update search with gather information, U.S. Pat 4450908 (" reduce polymer flooding biodegradable technology ") has been introduced and has been driven the back at biopolymer and inject various microorganisms, its defective is a mobility control difficulty of injecting microorganism.Another one is that Osage county North Burbank has carried out in the development zone the guiding test that microorganism is improved in-place permeability in the Oklahoma in the Phillips oil company, and this oil field bottom hole temperature (BHT) is 45 ℃.One of reason of selecting this oil field is that the eighties was once successfully implemented polyacrylamide/citric acid polymer treatment in this oil field, and its units increased in production is 9%.It is to utilize the interior indigenous microorganism of oil reservoir to inject nutritional agents continuously to make its activation that the microorganism of Pillips oil company is improved crude oil production patent technology; But its result is report (Slowing production decline andextending the economic life of an oil field:new MEOR technology.Brown L R, VadieA A.SPE59306) not.
Utilize the instance of microbial flooding field test to be merely during Dagang Oilfield year March October to 1999 nineteen ninety-five the pilot field test that well group after 7 polymer floodings adopted are annotated in Xisi, port district 3 has carried out microbial flooding behind the domestic polymer flooding; Homemade bacterium (Dagang Oilfield microbial oil displacement exploratory development development of projects reinjects behind i.e. elder generation's injection Micro-Bia bacterial classification BB; Liu Jinfeng; Liability company of Dagang Oilfield group, 1997.8).
Analyze investigation data both domestic and external; Discovery microbe oil production technology has been passed through the development of over half a century so far from nineteen twenty-six; But utilizing microorganism/chemical flooding further to improve the recovery ratio exploratory development after polymerization is driven is the most advanced and sophisticated cutting edge technology of comparison, no matter desk research or field trial; Only be to carry out preliminary exploration, also do not study rule and the method that can follow.And, still be faced with a lot of difficult problems how with microorganism/chemical flooding good combination and application.
Summary of the invention
The purpose of this invention is to provide the bacterium liquid that makes full use of preferred strain, with microorganism/chemical flooding good combination, with the microbe oil production method of oil recovery factor behind the raising polymer flooding.
Can be used for the preferred strain among the present invention; Include but not limited to fusiformis bacillus (Lysinibacillusfusiformis) 6#; This bacterial strain was preserved in the China Committee for Culture Collection of Microorganisms common micro-organisms center that is positioned at Da Tun road, Chaoyang District, BeiJing, China on 06 06th, 2008, and deposit number is CGMCC No.2439; Potsdam bacillus brevis strain is (Brevibacillus borstelensis;) Po; This bacterial strain was preserved in the China Committee for Culture Collection of Microorganisms common micro-organisms center that is positioned at Da Tun road, Chaoyang District, BeiJing, China on 06 06th, 2008, and deposit number is CGMCC No.2441; Lichem bacillus strain is (Bacilluslicheniformis) U1-3; This bacterial strain was preserved in the China Committee for Culture Collection of Microorganisms common micro-organisms center that is positioned at Da Tun road, Chaoyang District, BeiJing, China on 06 06th, 2008, and deposit number is CGMCC No.2437.
Among the present invention, said bacterium liquid is the mixed liquor or the zymotic fluid of above bacterial strain and nutrition base; The nutrition base can directly be the water of pH6.8-7.5, or presses the nutrient solution of following composition preparation: K
2HPO
40.1-0.5%, KH
2PO
40.1-0.5%, NH
4NO
30.1-0.2%, MgSO
47H
2O 0.01-0.1%, FeSO
40.001-0.01%, CaCl
20.001-0.01%, ZnSO
40.01-0.02%, CuSO
4, 0.0005-0.001%, urea 0.01-0.1%, crude oil 1-10%, polymer (molecular weight 1,400 ten thousand) 0.05-0.1%, all the other are water.Ratio of weight and number was 1-10:100 when bacterial strain and nutrition base mixed.
In the oil recovery process of the present invention, can use the single bacterium liquid of said bacterial strain, or use their compatibility bacterium liquid.When using compatibility bacterium liquid, can the single bacterium liquid of fusiformis bacillus, Potsdam bacillus brevis, bacillus licheniformis be pressed arbitrary proportion and mix, compound proportion is preferably 1:1:1 by weight.
Microbe oil production method scheme one detailed process of the present invention is: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, kept 7 days, last water drive.
Microbe oil production method scheme two detailed processes of the present invention are: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, and polymer injection protection slug kept 7 days again, last water drive.
Microbe oil production method scheme three detailed processes of the present invention are: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, annotate the ternary built oil displacement system again, polymer injection protection slug kept 7 days then, last water drive.
Wherein, and first polymer injection (1000mg/L) 0.57PV (molecular weight 1,400 ten thousand of polymer, solid content 0.88%, degree of hydrolysis 23mol%, viscosity is 50.2mPas); The bacteria concentration of the bacterium liquid that injects after the water drive is diluted to 10
6-7Individual/mL, inject bacterium liquid measure 0.2PV; Ternary built oil displacement system injection rate 0.3PV; Polymer injection 0.2PV (viscosity 50.2mPas) drives as the protection slug again; The prescription of ternary built system is: table agent sodium alkyl benzene sulfonate 0.1% alive, NaOH1.0%, polymer poly acrylamide 0.25%.
Among the present invention, fusiformis bacillus (Lysinibacillus fusiformis) 6#CGMCC No.2439 is a Gram-positive bacillus, and cell dia≤1 μ m forms gemma, and gemma expands, and non-circular; Its bacterium colony is creamy white, diameter 0.6-1.2 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 40-45 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=5-9, optimal pH=7.2; Its main biochemical characteristic is listed in table 1.
Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441 is a Gram-positive bacillus, the electron microscopic observation result, and cell dia≤1 μ m forms gemma, and non-circular; Its bacterium colony is creamy white, diameter 0.8-1.2 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 45-50 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=6.4-7.8, optimal pH=7.0.
Lichem bacillus strain (Bacillus licheniformis) U1-3 CGMCC No.2437 is a Gram-positive bacillus, the electron microscopic observation result, and cell dia≤1 μ m forms gemma, and gemma does not expand, and non-circular; Its bacterium colony is creamy white, diameter 0.6-1.5 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 45-50 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=5-9, optimal pH=5.7.
The present invention utilizes fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439, Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441, lichem bacillus strain (Bacillus licheniformis) U1-3 CGMCC No.2437 to carry out the biological displacement of reservoir oil.These bacterium are the samplings of polymer flooding piece from the grand celebration oil recovery factory, and through repeatedly shaking table experiment, plate streaking separates, and again bacterial classification is carried out performance evaluation, the polymer capable of using that therefrom optimizes and the bacterial classification of crude oil.These bacterium can be under the condition that polymer and crude oil exist growth and breeding; And be the energy and the carbon source of growth with crude oil and polymer; Utilize organic acid, the organic solvent of bacterium self-replacation and metabolism, the character of surfactant isoreactivity substance change crude oil simultaneously; It is exploited more easily, increase crude output.The model oil displacement experiment is the result show; Behind polymer flooding, improve the recovery ratio amplitude and reach 3-5% (OOIP) with these bacterium displacement of reservoir oils; Behind polymer flooding, drive the displacement of reservoir oil of addition polymerization compound protection slug and improve the about 7-9% of recovery ratio amplitude (OOIP) with this bacterium; Add ternary and drive combination and drive with this bacterium behind the polymer flooding, protect slug with polymer more afterwards, can improve the recovery ratio amplitude can reach about 13% (OOIP); Model experiment shows better repeatability, and the method for therefore utilizing microbial flooding and chemical flooding to combine has proposed a new way for probing polymer drives back raising recovery ratio; The expansion fermenting experiment result of bacterial classification proves that this bacterial classification can reach the production target of design, can guarantee to produce in batches.Above-mentioned experimental result shows that the present invention can be widely used in particularly microbial enhanced oil recovery field, petroleum production engineering field, suits large area to popularize and uses.
Below in conjunction with specific embodiment the present invention is explained further details.
Description of drawings
Figure 1A is the oil displacement efficiency figure of microorganism behind the polymer flooding of Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441
Figure 1B is the oil displacement efficiency figure of microorganism behind the polymer flooding of fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439
Fig. 1 C is the oil displacement efficiency figure of microorganism behind the polymer flooding of bacillus licheniformis (Bacillus licheniformis) U1-3 CGMCC No.2437
Fig. 1 D is the oil displacement efficiency figure of microorganism behind the polymer flooding of compatibility bacterium
Fig. 2 A is the rheological curve figure of the forward and backward crude oil of bacillus licheniformis (Bacillus licheniformis) U1-3 CGMCC No.2437 injection model
Fig. 2 B is the rheological curve figure of the forward and backward crude oil of fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439 injection model
Fig. 2 C is the rheological curve figure of the forward and backward crude oil of Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441 injection model
Fig. 2 D is the rheological curve figure of crude oil before and after the compatibility bacterium liquid injection model
The specific embodiment
Method therefor is conventional method if no special instructions among the following embodiment.
The screening of embodiment 1, bacterial strain, cultivation and preservation
Enriched medium: K
2HPO
40.1-0.5%, KH
2PO
40.1-0.5%, NH
4NO
30.1-0.2%, MgSO
47H
2O0.01-0.1%, FeSO
40.001-0.01%, CaCl
20.001-0.01%, ZnSO
40.01-0.02%, CuSO
4, 0.0005-0.001%, urea 0.01-0.1%, yeast soak powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 1,400 ten thousand) 0.05-0.1%, all the other are water, pH 6.8-7.5,121 ℃, sterilization 15-20min.45 ℃ of 120rpm shaking tables were cultivated 5-7 days.
Oil plating medium: K
2HPO
40.1-0.5%, KH
2PO
40.1-0.5%, NH
4NO
30.1-0.2%, MgSO
47H
2O0.01-0.1%, FeSO
40.001-0.01%, CaCl
20.001-0.01%, ZnSO
40.01-0.02%, CuSO
4, 0.0005-0.001%, urea 0.01-0.1%, yeast soak powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 1,400 ten thousand) 0.05-0.1%, agar 1.5-2%, all the other are water, pH6.8-7.5,121 ℃, sterilization 15-20min.
Slant medium: peptone 1%, beef extract 0.3%, sodium chloride 0.5%, all the other are water, pH7.0-7.2.
The bacterial classification that will screen not only must adapt to temperature, pressure, PH, the salinity of oil reservoir and detest ambient conditionss such as foster; Also must have outstanding degradation polymer and the ability of utilizing crude oil; With following method to satisfactory bacterial strain screen, cultivation and preservation, concrete grammar may further comprise the steps:
1, the screening of bacterial strain
From 256 of each oil recovery factory polymer flooding piece samplings of Daqing oil field, measured in ability that bacterial classification utilizes crude oil and polymer and the oil-reservoir water characteristics such as origin bacterium compatibility with method such as eliminate, optimize at last under the condition that oil reservoir, polymer solution and crude oil exist, to survive, growth and breeding; And but metabolism produces organic acid, active material, degrading crude oil to improve the bacterial strain of recovery ratio; Concrete grammar is: earlier carry out enrichment culture with enriched medium, cultivated 7 days down at 45 ℃, utilize the characteristic that microorganism can emulsified crude oil then; The enrichment culture thing is inoculated on the oily plating medium with the method that line separates; Under 45 ℃, place 400 type anaerobic box to cultivate 3-5 days, observe colonial morphology then, select the single bacterium colony that dissolves oil (having the oil extraction activity) and do further research; The result is through more than 300 shaking table experiment; Initial gross separation goes out 142 strain bacterium behind the plate streaking, and the single colony inoculation that will select from oily flat board then places Switzerland concept400 type anaerobism incubator to cultivate under 45 ℃ 3-5 days on slant medium; Filter out three strains of purpose bacterial strain, all belong to facultative anaerobe.Comprising:
The bacterial strain of called after 6#: through identifying that its 16S rDNA has the nucleotide sequence of sequence 1 in the sequence table, proves that this bacterial strain is fusiformis bacillus (Lysinnibacillus fusiformis); It is Gram-positive bacillus; The electron microscopic observation result, cell dia≤1 μ m forms gemma; Gemma expands, and non-circular; Its bacterium colony is creamy white, diameter 0.6-1.2 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 40-45 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=6-9, optimal pH=7.2; Main biochemical characteristic is seen table 1.
The bacterial strain of called after Po: through identifying; Its 16S rDNA has the nucleotide sequence of sequence 2 in the sequence table, proves that this bacterial strain is Potsdam bacillus brevis (Brevibacillus borstelensis), is Gram-positive bacillus; The electron microscopic observation result; Cell dia≤1 μ m forms gemma, and non-circular; Its bacterium colony is creamy white, diameter 0.8-1.2 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 45-50 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=6.4-7.8, optimal pH=7.0; Main biochemical characteristic is as shown in table 1.
The bacterial strain of called after U1-3: through identifying that its 16S rDNA has the nucleotide sequence of sequence 3 in the sequence table, proves that this bacterial strain is lichem bacillus strain (Bacillus licheniformis); It is Gram-positive bacillus; The electron microscopic observation result, cell dia≤1 μ m forms gemma; Gemma does not expand, and non-circular; Its bacterium colony is creamy white, diameter 0.6-1.5 μ m; This bacterium grows under the amphimicrobian condition, growth temperature range: 45-50 ℃, and optimum growth temperature: 45 ℃; Growth acid-base value scope: pH=5-9, optimal pH=5.7; Main biochemical characteristic is as shown in table 1.
The part biochemical characteristic of table 1 three strain bacterium
| Pilot project | Po result | 6# result | U1-3 result |
| Catalase | + | + | + |
| Oxidizing ferment | - | - | + |
| The VP test | - | - | + |
| VP<pH?6 | - | - | - |
| VP>pH?7 | - | - | - |
| The MR test | + | - | + |
| Utilize citrate | + | - | + |
| 7% NaCl growth | - | - | + |
| Utilize glucose to produce acid | - | + | + |
| Utilize wood sugar to produce acid | - | + | + |
| Utilize the L-arabinose to produce acid | - | + | + |
| Utilize sweet mellow wine to produce acid | - | - | + |
| Nitrate reduction | + | - | + |
| The starch hydrolysis | - | - | + |
| Gelatin liquefaction | + | - | + |
| Decompose casein | + | - | + |
Annotate: "+" expression reacting positive; "-" expression reaction negative.
2, the cultivation of bacterial strain and preservation
Cultivate: bacterial strain to shake bottle culture medium identical with enriched medium, condition of culture is under 45 ℃, 150rpm shaken cultivation 7-15 days., shaking table can adopt two kinds of methods in cultivating; With the triangular flask that culture medium is housed after aseptic preparation; A kind of is to observe the growing state of bacterium and the degree of emulsified crude oil in the shaking table shaken cultivation; Another kind is nutrient solution to be put into the anaerobism incubator regularly let bacterium fully contact with crude oil with the hand vibration every day, observes the change color situation of bacterial action crude oil and zymotic fluid simultaneously.This bacterial classification can be grown under above-mentioned two kinds of cultural methods as a result, proves that further the bacterial strain that is screened belongs to facultative anaerobe.Bacterial strain fermentation liquor is used for follow-up oil displacement experiment as bacterium liquid.
Preservation: this three bacterial strain was preserved in the China Committee for Culture Collection of Microorganisms common micro-organisms center that is positioned at Da Tun road, Chaoyang District, BeiJing, China on 06 06th, 2008; Deposit number is respectively fusiformis bacillus (Lysinibacillus fusiformis) 6# CGMCC No.2439; Potsdam bacillus brevis strain (Brevibacillus borstelensis;) Po CGMCC No.2441, lichem bacillus strain (Bacilluslicheniformis) U1-3 CGMCC No.2437.
3, bacterial classification performance
Have in the oil reservoir anaerobism under the condition of polymer and crude oil existence through simulation, these bacterial classifications have been carried out 18 assays experiments, experimental result shows:
1) bacterial classification can utilize polymer and crude oil to breed for carbon source for growth, and under reservoir condition well-grown;
2) through performances such as the interfacial tension of this strain fermentating liquid, zymotic fluid organic acid content are estimated; Confirm this bacterial classification ability oxidative degradation crude oil and polymer, its viscosity is descended, and have the ability of the acid of producing, surfactant; Variation has taken place in oil property after this bacterial classification effect; The chromatography result proves that light component increases, and heavy component reduces relatively, ∑ C
21/ ∑ C
22Ratio has increased by 27.0%, C
21+ C
22/ C
28+ C
29Ratio has increased by 35.1%; Interfacial tension between profit is reduced to 12.89mN/m by the 35.38mN/m of blank; The organic acid content of bacterial classification metabolism rises to 852mg/L by 300mg/L, and the acid number of crude oil is increased to 0.3381mg (KOH)/g by 0.015mg (KOH)/g after the bacterial classification effect, and acid number has improved 21.54 times; Paraffin content has reduced by 39.2%, gel content has descended 1.17%, freezing point has reduced by 34.15%, and Engler distillation just flow point has reduced by 80 ℃;
3) with newton's index, viscosity variable quantity, the characteristic of this bacterial classification that improved the recovery ratio index assessment; The result shows that this bacterial classification has changed oil property; Particularly changed the mobile performance of crude oil effectively, made after the bacterial classification effect character of crude oil shift to Newtonian fluid, viscosity variable quantity parameter is higher; Relative raising recovery ratio index is also higher, can think that this bacterial classification utilizes the better performances of crude oil;
4) be the characteristic that this bacterial classification of overall merit adapts to oil reservoir, also carried out compatibility, pH value and toxicity test with oil reservoir origin bacterium, this bacterial classification is under the condition of polymer existence as a result, and viable count reaches 10
9-11Individual/mL, and the viscosity of polymer reduced to 1.00mPas by 18.6mPas.
Experimental model: research vehicle is artificial inhomogeneous consolidated core, coefficient of variation Vk=0.72, the long cm=31.1-31.3 of model, sectional area cm
2=12.38-12.46.
Experimental water, oil, bacterium liquid:
The saturation water of experiment usefulness is that (prescription is: NaCl 0.3977% CaCl by the artificial salt solution of preparing of the average formation water of Daqing oil field
20.0028%, MgCl
26H2O 0.0046%, Na
2SO
40.0093%, NaHCO
30.2634%), salinity is 6778mg/L.
Experiment is to take from the recover the oil crude oil of the Bei2Qu of three factories-J5--P25 well of grand celebration with oil, and the viscosity of crude oil is 47.2mPas after dewatering.
Inject water and be the recover the oil injection water of three factory's polymer injection stations of grand celebration.
Bacterium liquid is used in experiment: the single bacterial strain fermentation liquor that is each bacterial strain described in the embodiment 1.Or the pH value of using bacterial strain and water directly to prepare is the 5.0-9.0 aqueous solution.Or the zymotic fluid of three kinds of bacterium is by the fusiformis bacillus: Potsdam bacillus brevis: bacillus licheniformis is the compatibility bacterium liquid that 1:1:1 mixes.
Polymer is used in experiment: be polyacrylamide.
Experiment one: this experiment is the rock core to water drive behind the polymer injection, directly annotates the microbial oil displacement experiment.
Experimental implementation is followed successively by: the rock core saturated formation water of finding time, inject saturated oils, and water drive is moisture to reach 100%; Polymer injection (1000mg/L) 0.57PV (molecular weight 1,400 ten thousand of polymer, solid content 0.88%, degree of hydrolysis 23mol%; Viscosity is 50.2mPas), water drive is moisture to reach 100%; The bacteria concentration of bacterium liquid is diluted to 10 respectively
6-7Individual/mL, annotate bacterium liquid 0.2PV then, 45 ℃ of constant temperature kept 7 days, and water drive is moisture again reaches 100%, finishes experiment.
The per two blocks of rock cores of oil displacement experiment are one group of parallel appearance, have done two group model oil displacement experiments altogether.In addition, will from the microbial oil displacement model, displace the crude oil centrifugal dehydration and the injection model crude oil before that come and do the rheological characteristic analysis, the viscosity of displacing between next crude oil in crude oil before the observation injection model and the model changes.
The microbial oil displacement effect is shown in Figure 1A～1D behind the polymer flooding, and the microbial oil displacement model data is as shown in table 2 behind the polymer flooding, and directly the water drive model data is as shown in table 2 behind the polymer.
Microbial oil displacement model data summary sheet behind table 2 polymer flooding
| Numbering | Bacterium liquid | Voids volume mL | Degree of porosity % | Permeability * 10-3 μ m 2 | Oil saturation % | Waterflood recovery efficiency factor % | Polymer EOR | MEOR％ | |
| 1 | Water drive | 95.05 | 24.56 | 1338 | 74.69 | 40.85 | 11.26 | 0.00 | |
| 1 | #6 | 90.57 | 23.46 | 1138 | 71.76 | 32.30 | 15.38 | 4.18 | |
| 2 | #6 | 95.71 | 24.79 | 1209 | 68.74 | 44.98 | 15.20 | 3.03 | |
| 1 | Po | 101.74 | 25.79 | 1256 | 66.98 | 40.56 | 14.99 | 4.09 | |
| 2 | Po | 96.09 | 24.07 | 1189 | 69.52 | 46.73 | 14.79 | 3.84 | |
| 1 | U1-3 | 92.56 | 27.89 | 1146 | 68.24 | 45.77 | 14.87 | 4.20 | |
| 2 | U1-3 | 99.07 | 27.33 | 1278 | 70.05 | 43.56 | 15.47 | 3.72 | |
| 1 | The compatibility bacterium | 90.18 | 23.36 | 1164 | 67.64 | 43.44 | 14.75 | 4.8 | |
| 2 | The compatibility bacterium | 90.01 | 23.32 | 1195 | 77.76 | 36.57 | 14.28 | 5.51 |
Can find out by table 2 model experiment data; The amplitude that two parallel appearance of fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439 are improved recovery ratio is 3.03-4.18%; The amplitude that two parallel appearance of Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441 are improved recovery ratio is 3.84-4.09%; The amplitude that two parallel appearance of bacillus licheniformis (Bacillus licheniformis) U1-3 CGMCC No.2437 are improved recovery ratio is 3.72-4.20%, and the amplitude that two parallel appearance of compatibility bacterium appearance are improved recovery ratio is 4.8-5.51%; Analyzing polymers drives and moisturely drops to 80% by 100% from model curve figure, and microbial flooding is moisture to be dropped to about 90% by 100%, shows after bacterium liquid injects to make moisture reduction that recovery ratio improves.
Experimental result is analyzed, and the pH value of injecting water is 8, and the pH value of output water is 5-6 from model; When the follow-up water drive of model, observed the growth conditions of three bacterial classifications in model: viable count is 10 when model outlet output liquid 10mL
8Individual/mL, viable count is 10 during output liquid 45mL (0.5PV)
9Individual/mL, viable count is 10 during the terminal output liquid 90mL of model (1PV)
8Individual/mL.Viable count explanation bacterial migration from rock core output liquid is to whole rock core, because model is annotated bacterium liquid 0.2PV, in fact bacterium liquid only gets into 1/5 place of model inlet, shifts to new food polymer carbon source behind near the nutrients bacterial classification consumes and carries out growth metabolism.
The rheological characteristic analysis result is shown in table 3 and Fig. 2 A～2D, and viscosity of crude descends along with the increase of shear rate after the effect of bacterium liquid, and viscosity break ratio reaches more than 50% basically; Show that bacterium liquid has oil displacement efficiency preferably.
The relation of forward and backward viscosity of crude of table 3 bacterium liquid injection model and shear rate
Experiment two: this experiment is the rock core to water drive behind the polymer injection, annotates microorganism earlier, again polymer injection protection slug oil displacement experiment.
(A) experimental implementation is followed successively by: the rock core saturated formation water of finding time, inject saturated oils, and water drive is moisture to reach 100%; Polymer injection (1000mg/L) 0.57PV (molecular weight 1,400 ten thousand of polymer, solid content 0.88%, degree of hydrolysis 23mol%; Viscosity is 50.2mPas), water drive is moisture to reach 100%; The bacteria concentration of bacterium liquid is diluted to 10 respectively
6-7Individual/mL, annotate bacterium liquid 0.2PV then, to annotate 0.2PV polymer viscosity (50.2mPas) protection slug again and drive, 45 ℃ of constant temperature kept 7 days, and water drive is moisture at last reaches 100%, finishes experiment.
(B) reduce the protection slug and drive polymer viscosity, experimental implementation is followed successively by: the rock core saturated formation water of finding time, inject saturated oils; Water drive is moisture to reach 100%; Polymer injection (1000mg/L) 0.57PV (molecular weight 1,400 ten thousand of polymer, solid content 0.88%, degree of hydrolysis 23mol%; Viscosity is 22.4mPas), water drive is moisture to reach 100%; The bacteria concentration of three kinds of bacterium liquid is diluted to 10
6-7Individual/mL, annotate bacterium liquid 0.2PV then, to annotate 0.2PV polymer viscosity (22.4mPas) protection slug again and drive, 45 ℃ of constant temperature kept 7 days, and water drive is moisture at last reaches 100%, finishes experiment.
Use (A) and respectively carried out the parallel oil displacement experiment of artificial core with (B), experimental result is as shown in table 4.
Microbial flooding behind table 4 polymer flooding-polymer protection slug oil displacement experiment data
| Bacterium liquid | Voids volume mL | Degree of porosity % | Permeability * 10 -3μm 2 | Oil saturation ratio % | Waterflood recovery efficiency factor % | Polymer EOR% | Slug % behind the MEOR+ |
| #6(A) | 91.02 | 23.10 | 1305 | 72.5 | 39.41 | 15.90 | 9.85 |
| #6(B) | 92.01 | 23.14 | 1281 | 73.6 | 38.97 | 11.02 | 7.35 |
| Po(A) | 93.86 | 23.90 | 1329 | 75.6 | 40.14 | 16.91 | 11.97 |
| Po(B) | 93.86 | 23.90 | 1329 | 75.6 | 38.21 | 14.31 | 10.08 |
| U1-3(A) | 97.09 | 24.16 | 1254 | 77.35 | 36.49 | 14.99 | 9.58 |
| U1-3(B) | 94.00 | 23.98 | 1326 | 73.4 | 41.30 | 10.14 | 6.95 |
| Compatibility (A) | 93.25 | 24.01 | 1346 | 71.25 | 40.69 | 12.35 | 13.51 |
| Compatibility (B) | 92.58 | 23.68 | 1295 | 73.41 | 41.23 | 14.10 | 12.03 |
Can find out that from the contrast of table 4 and table 2 data it is more obvious to test two recovery ratio increase rates, the oil displacement efficiency of experiment two is better than experiment one.Main cause is after annotating microorganism, to have added polymer protection slug, and the crude oil behind microbial action can evenly be advanced by polymer, and the crude oil behind the microbial action is displaced in the rich productive set, has improved swept volume, thereby has increased displacement of reservoir oil amplitude.
To test two (A) and experimental data (B) compares; (A) the oil recovery amplitude of EOR% and MEOR% all is higher than (B), and this mainly is because (A) the injection viscosity (50.6mPas) of polymer solution is higher than due to the injection viscosity (22.4mPas) of polymer solution of (B).
In the process of experiment, also detected the viscosity of the forward and backward polymer solution of injection model.The original viscosity that the result tests the preflood polymer solution of two (A) models is 50.2mPas; Slug is that in the process of injection model, to connect the viscosity that from model output liquid 0.5PV detects polymer with the 50mL graduated cylinder be 16.5mPas to 0.57PV; Polymer solution filters back viscosity through porous media and has descended more than 3 times; Viscosity when displacing 1PV is 2.0mPas, and the viscosity when continuing to displace 1.5-2PV is 0.0mPas, shows that polymer has been produced absorption and delay by rock and pore throat in oil reservoir.
Experiment three: this experiment is the rock core to water drive behind the polymer injection, annotates microorganism earlier, annotates chemical oil displacement agent again, then polymer injection protection slug oil displacement experiment.
Experimental implementation is followed successively by: the rock core saturated formation water of finding time, inject saturated oils, and water drive is moisture to reach 100%; Polymer injection (1000mg/L) 0.57PV (molecular weight 1,400 ten thousand of polymer, solid content 0.88%, degree of hydrolysis 23mol%; Viscosity is 50.2mPas), water drive is moisture to reach 100%; The bacteria concentration of bacterium liquid is diluted to 10
6-7Individual/mL, annotate bacterium liquid 0.2PV then, annotate the ternary system 0.3PV (prescription of ternary system: table agent sodium alkyl benzene sulfonate 0.1% alive again; NaOH1.0%; Polymer poly acrylamide 0.25%), annotate 0.2PV polymer viscosity (50.2mPas) protection slug then and drive, 45 ℃ of constant temperature kept 7 days; Water drive is moisture at last reaches 100%, finishes experiment.
For the amplitude of Microbial Model oil displacement experiment relatively, done to gather with artificial heterogeneous core and driven the back and directly annotate the 0.3PV ternary system and carry out the displacement of reservoir oil, after annotate the displacement of reservoir oil check experiment that the 0.2PV polymer is protected slug again, experimental result is as shown in table 5.
Behind table 19 polymer flooding+microorganism+chemical flooding+polymer protection slug model experiment data
| Bacterium liquid | Voids volume ml | Degree of porosity % | Permeability * 10 -3μm 2 | Oil saturation ratio % | Waterflood recovery efficiency factor % | Polymer EOR% | MEOR+ ternary+back |
| # | |||||||
| 6 | 75.30 | 20.26 | 1317 | 67.07 | 41.58 | 10.89 | 16.56 |
| Po | 78.28 | 18.69 | 1368 | 66.90 | 40.34 | 10.66 | 16.90 |
| U1-3 | 73.59 | 20.56 | 1325 | 67.89 | 42.12 | 9.85 | 15.19 |
| The compatibility bacterium | 75.10 | 21.84 | 1351 | 68.91 | 43.25 | 11.23 | 17.53 |
| Chemical flooding | 105.96 | 22.43 | 1345 | 77.38 | 41.46 | 10.36 | 3.66 |
See gathering and drive the back and has annotated the microorganism continued and annotate chemical flooding and deduct 3.66% of check experiment that raising recovery ratio amplitude is about 13% from above-mentioned experimental data.
Sequence table
<160>3
<210>1
<211>1446
<212>DNA
< 213>fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439
<400>1
<210>2
<211>1437
<212>DNA
< 213>Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441
<400>1
<210>3
<211>1459
<212>DNA
< 213>bacillus licheniformis (Bacillus licheniformis) U1-3 CGMCC No.2437
<400>1
Claims (8)
1. a microbe oil production method is included in after the oil reservoir polymer flooding, the process of using specified germ liquid to carry out the displacement of reservoir oil, and the bacteria concentration of injection bacterium liquid is 10
6-7Individual/mL, injection rate is 0.2PV; Said specified germ liquid is fusiformis bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439, Potsdam bacillus brevis (Brevibacillus borstelensis) Po CGMCC No.2441 or the single bacterial strain of bacillus licheniformis (Bacillus licheniformis) U1-3 CGMCC No.2437 and the mixed liquor or the zymotic fluid of nutrition base, or single bacterial strain mixes the compatibility bacterium liquid that forms with the mixed liquor or the zymotic fluid of nutrition base; Said nutrition base is the water of pH6.8-7.5, or presses the nutrient solution of following composition preparation: K
2HPO
40.1-0.5%, KH
2PO
40.1-0.5%, NH
4NO
30.1-0.2%, MgSO
47H
2O 0.01-0.1%, FeSO
40.001-0.01%, CaCl
20.001-0.01%, ZnSO
40.01-0.02%, CuSO
4,0.0005-0.001%, urea 0.01-0.1%, yeast soak powder 0.02-0.2%, crude oil 1-10%, the polymer 0.05-0.1% of molecular weight 1,400 ten thousand, all the other are formed for water; Said bacterial strain and nutrition basic weight amount portion rate are 1-10: 100.
2. microbe oil production method according to claim 1 is characterized in that, detailed process is: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, kept 7 days, last water drive.
3. microbe oil production method according to claim 1 is characterized in that, detailed process is: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, and polymer injection protection slug kept 7 days again, last water drive.
4. microbe oil production method according to claim 3 is characterized in that, the polymer viscosity of polymer protection slug is at 22.6～50.2mPas.
5. microbe oil production method according to claim 1 is characterized in that, detailed process is: behind the oil reservoir polymer flooding, first water drive is moisture to reach 100%; Bacterium liquid is directly injected oil reservoir, annotate the ternary built oil displacement system again, polymer injection protection slug kept 7 days then, last water drive.
6. microbe oil production method according to claim 5 is characterized in that, the prescription of said ternary built oil displacement system is: table agent sodium alkyl benzene sulfonate 0.1% alive, NaOH1.0%, polymer poly acrylamide 0.25%.
7. according to the arbitrary described microbe oil production method of claim 3 to 6, it is characterized in that said polymer protection slug injection rate 0.2PV.
8. according to claim 5 or 6 described microbe oil production methods, it is characterized in that said ternary built oil displacement system injection rate 0.3PV.
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