CN1540136A - Method for estimating capability of edge-water encroachment of microbe - Google Patents
Method for estimating capability of edge-water encroachment of microbe Download PDFInfo
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- CN1540136A CN1540136A CNA2003101082034A CN200310108203A CN1540136A CN 1540136 A CN1540136 A CN 1540136A CN A2003101082034 A CNA2003101082034 A CN A2003101082034A CN 200310108203 A CN200310108203 A CN 200310108203A CN 1540136 A CN1540136 A CN 1540136A
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Abstract
A method for evaluating the oil displacing power of microbes includes filling the saline rich in nutrients and microbes in the bore bearing the residual oil after water displacement, slealing the both ends of said bore for culturing microbes, displacing to output fluid, repeating previous two steps utile no crude oil can be recovered, and calculating the ampletude of the covered rate of crude oil displaced by microbes.
Description
Technical field
The present invention relates to a kind of evaluation method, be specifically related to microbial oil displacement ability assessment method in the oil extraction process.
Background technology
Microbial flooding is a kind of economy, effectively improves the oil recovery factor technology, and this technical research originates in the '20s in last century.Nineteen twenty-six, the graceful imagination that at first proposes " utilizing microorganism to improve oil recovery factor " of Buick at oil reservoirs; Assistant Bel in 1940 finds that microorganism can discharge from core oily, and obtained anaerobic bacteria is injected into the patent of oil reservoir in nineteen forty-six with American Petroleum Institute; 1954 the beginning, in Eastern Europe some the country as: the former Soviet Union, Poland, Hungary, Czechoslovakia have carried out field test in succession.Entered since the eighties, countries in the world are more and more paid attention to the microbe oil production technology, and deeply, the field test scale increases desk research year by year, and sees gratifying test effect, has shown that microbial oil displacement has the commercial Application promotional value gradually.
The dominant mechanism of microbial oil displacement is: 1. the heavy component in the microorganism energy cracking crude oil produces the lightweight component, thereby reduces viscosity of crude; 2. gases such as microbial metabolism carbon dioxide, methane can increase strata pressure, and under formation condition, this gas can reduce viscosity of crude after being dissolved in crude oil, increase crude oil fluidity; 3. microbial metabolism acidic materials solubilized carbonate rock increases the mobile duct of profit; 4. the microbial metabolism biosurfactant can reduce oil water interfacial tension, and emulsified crude oil improves displacement efficiency; 5. the microbial metabolism biopolymer can be adjusted intake profile expansion injection ripples and volume; 6. organic solvents such as microbial metabolism organic acid, alcohol improve oil displacement efficiency or the like.Through a large amount of research and field test, the mentioned microorganism mechanism of oil displacement has obtained comparatively general approval, and with the carrying out of desk research, understanding deepens continuously.
The research of microbial oil displacement generally comprises the research of the exploitation of oil extraction microbial bacterial classification, growth of microorganism and metabolism performance study, microbial degradation performance of crude oils, microorganism and changes rock wettability and can study etc., and the microbial oil displacement ability is the combined reaction of growth of microorganism metabolism, degrading crude oil, change rock wettability energy.
The microbial oil displacement ability assessment is that microorganism fungus kind exploitation and mining site are used the core link of (using for mining site provides key parameter such as inoculum density, microorganism consumption etc.).At present, the microbial oil displacement ability assessment is generally undertaken by rock core simulation with reference to the chemical agent oil removing evaluation method, and its key step is: 1. behind the core saturated oils water drive to residual oil; 2. microbial solution is injected core; 3. core sealing, cultivation (general about 7 days); 4. recover water drive, metering oil recovery factor improvement value, with the increase rate of oil recovery factor as microbial oil displacement ability assessment parameter.
The inventor finds through a large amount of experimental studies, because the microorganism concn that is inoculated in the rock core is lower by (general 10
6～10
7Cells/ml), growing environment changes, microorganism enter behind the rock core activity also a little less than.It is active that microorganism could breed gradually, recover after will tiding over certain laundering period.After microbial growth reached certain concentration, the effect of microbial oil displacement just can strengthen significantly, at this moment, improved the oil recovery factor effect and just showed gradually.Because microbial reproduction needs the regular hour to enough concentration, the process that microbial flooding starts crude oil is slowly, it is the process that progressively accumulates, therefore when utilizing the rock core simulated experiment to estimate the microbial oil displacement ability, showing that upward there is a time lag in microbial oil displacement ability (extraction crude oil, raising oil recovery factor amplitude).
This shows, the present microbial oil displacement ability assessment method that adopts, after rock core sealing, short time cultivation, carry out water drive and just finish experiment, this evaluation method exists a lot of drawbacks:, nonnutritive liquid short as incubation time replenishes, does not consider that effect, the microorganism concn of microbial oil displacement time lag is low can not reflect oil displacement efficiency after the microbial reproduction fermentation etc., thereby does not reflect the ability of microbial oil displacement fully.This has not only influenced the concentration to microbial inoculant, the apolegamy of nutrient solution, the accurate evaluation of microbial oil displacement ability, even causes very mistake on judgement microbial performance quality and bacterial screening; In the mining site application facet, the prediction and the early stage that have influenced definite and the optimization and the test effect of field test key parameter such as microorganism implantation concentration etc. are estimated.
Summary of the invention
The technical issues that need to address of the present invention are to disclose a kind of method of estimating the microbial oil displacement ability, to overcome above-mentioned a series of defectives that prior art exists, satisfy microorganism strains filtration, the needs of mining site application parameter are provided, final for the mining site application filters out the bacterial classification of high-quality and parameter accurately, satisfy the needs of oilfield exploitation.
Method of the present invention comprises the steps:
(1) rock core adopts conventional method saturation water, mother oil displacement to set up irreducible water saturation; The water drive rock core is set up residual oil saturation; In rock core, inject then contain microorganism and match with said microorganism contain nutraceutical salt solution, microorganism concn is 10
6～10
7Cells/ml.
Said microorganism is the 1. degrading crude oil of microorganism with following one or more performances; 2. metabolism surfactant; 3. metabolism gas; 4. metabolism polymer; 5. organic solvents such as fat metabolism acid or alcohol; 6. metabolism other help improving the material of oil recovery factor.Microorganism can be that a kind of microorganism also can be the combination of two or more microorganisms.Said microorganism comprises aerobic bacteria, anaerobic bacteria or facultative anaerobe.
Said microorganism comprises following microorganism, but is not limited to these microorganisms:
Document: Makkar R S, Cameotra S S.Production of biosurfactant at mesophilicand thermophilic conditions by a strain of Bacillus subtilis.J.Ind.Microbiol.Biotechnol., 20 (1), 48-52,1998 disclosed bacillus subtilises (B.subtilis), document: YakimovM M, Amro M M, Bock M, et al.The potential of Bacillus licheniformis strains forin situ enhanced oil recovery.J.Pet.Sci.Eng., 18 (1/2), 147-160,1997 disclosed bacillus licheniformis (B.licheniformis), document: Nazina T N, Ivanova A E, Mityushina L L, et al.Thermophilic hydrocarbon-oxidizing bacteria from oil fields.Mikrobiologiya, 62 (3), 583～592,1993 disclosed happiness heat are bitten oily bacillus (B.thermoleovorans), document: and in causing, Peng Qian, Ma Jun, Chen Junying. the research of Yunnan hot spring high temperature bacterium: the extreme thermophilic bud pole bacterium in the acid high temperature hot spring of VII. Tengchong. microorganism journal 29 (3), 161-165,1989 disclosed bacillus stearothermophiluses (B.stearothermophilus), document: Gurerra-Santos L, Kappeli O, Fiechter A.Pseudomonasaeruginosa biosurfactant production in continuous culture with glucose as carbonsource.Appl.Environ.Microbiol.48,301-305,1984 disclosed pseudomonas aeruginosas (P.aeruginosa), document: Behlulgil K, Mehmetoglu M T.Bacteria for improvement of oilrecovery:a laboratory study.Energy Sources, 24 (5), 413-421,2002 disclosed clostridium acetobutylicums (C.acetobutylicum), document: Lappan R E, Fogler H S.Leuconostocmesenteroides, growth kinetics with application to bacterial profile modification.Biotechnol.Bioeng., 43 (9), 865-73,1994 disclosed Leuconostoc mesenteroides (L.mesenteroides), document: Post F J, Al-Harjan F A.Surface activity of halobacteria andpotential use in microbially enhanced oil recovery.Syst.Appl.Microbiological, 11 (1), 97-101,1988 disclosed salt bacillus (H.distribution), document: Milekhina E I, Borzenkov I A, Zvyagintseva I S, et al.Characterization of a hydrocarbon-oxidizing Rhodococcus erythropolis strain isolated from an oil field.Microbiology, 67 (3), 271-274, a kind of in the 1998 disclosed Rhodococcus erythropolis bacterium such as (R.erythropolis), preferred bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa, happiness heat is bitten oily bacillus and bacillus stearothermophilus.
(2) rock core is sealed at both ends, carries out the microorganism cultivation.Cultivation temperature is microbial growth temperature of being tested or the reservoir temperature that is used for field test, is generally 30～90 ℃, and the incubation time of microorganism is determined according to the microbial growth characteristic, was generally 1～10 day.
(3) after rock core is cultivated, open the pressure release of rock core port, adopt and inject water drive, also can directly adopt and be rich in nutraceutical salt solution displacement for then with being rich in nutraceutical salt solution displacement.Displacement velocity can be determined according to test reservoir fluid seepage velocity, be generally 1～10m/d.
(4) repeat (2)～(3) step, till not having the crude oil extraction, generally repeat 10～30 times;
(5) with the identical but core experiment not microbe inoculation of operation as contrast, calculate microbial flooding and improve the oil recovery factor amplitude, the amplitude that improves oil recovery factor with microbial flooding is as the parameter index of estimating the microbial oil displacement ability.
This experimental procedure (4) is carried out " rock core closed at both ends, cultivate microorganism, water drive and contain nutraceutical salt water drive " process repeatedly, is key link.This link has been utilized following principle: microorganism is absorption in a large number in rock core, has only the slow output of a small amount of microorganism; Constantly inject nutrient solution, satisfy the requirement of microorganism, stimulate the growth of microorganism breeding nutrient solution; Utilize the absorption of microorganism in rock core, cultivate repeatedly and impel microorganism in rock core, to reach sufficiently high concentration, performance displacement of reservoir oil effect; Prolong microorganism and crude oil, rock action time, reduced the influence of microbial oil displacement time lag etc.; Displacement repeatedly, the constantly crude oil of extraction microorganism startup.By this process of carrying out repeatedly, fully reflect the ability of microbial oil displacement.
The present invention proposes microbial oil displacement ability assessment method by injecting processes such as nutrients, sealing cultivation, displacement repeatedly, overcome series of malpractice of the prior art, the microorganism of injecting in the rock core is bred to greatest extent, oil displacement efficiency finds full expression, thereby therefore the ability that reflects microbial oil displacement truly is a kind of method of estimating the microbial oil displacement ability accurately, reliably.
The specific embodiment
Embodiment 1
Adopt artificial inhomogeneous rock core, water phase permeability is 0.6 μ m
2, displacement water is certain oilfield injection water through sterilization treatment, nutrient solution is by displacement water configuration (composition: NH
4Cl:0.3%, K
2HPO
4: 0.1%, yeast extract 0.05%, wt%), displacement velocity is 5m/d.
The rock core saturation water, mother oil displacement is to irreducible water saturation, injecting water drive replaces to residual oil saturation, metaideophone bacillus subtilis (Makkar R S then, Cameotra S S.Production of biosurfactant atmesophilic and thermophilic conditions by a strain of Bacillus subtilis.J.Ind.Microbiol.Biotechnol., 20 (1), 48-52,1998, bacillus subtilis B.subtilis) zymotic fluid (being diluted to concentration 5%) 1PV (PV with nutrient solution, the voids volume of test core), 5d is left standstill in sealing rock core two ends under 37 ℃ of experimental temperatures.
Replace to moisture (displacement 2PV) end experiment more than 98% of output liquid with injecting water drive by art methods, microbial flooding improves oil recovery factor 6.4%, and (oil recovery factor refers to the percentage of original saturated former oil mass in former oil mass of extraction and the rock core, improving the oil recovery factor value is under identical appreciation condition, and microbial flooding oil recovery factor value is poor with complete water drive oil recovery factor value).
Adopt the inventive method, carry out repeatedly " rock core closed at both ends, cultivate microorganism, water drive and contain nutraceutical salt water drive ", when not having the crude oil extraction, carried out 21 times altogether and handled, microbial flooding raising oil recovery factor 32.8%.
This shows that prior art does not reflect the ability of the microbial oil displacement of being tested fully.Adopt evaluation method of the present invention, the bacillus subtilis oil-displacement capacity that shows test is very high, improves the oil recovery factor amplitude and reaches 32.8%.
Claims (11)
1. a method of estimating the microbial oil displacement ability is characterized in that, comprises the steps:
(1) rock core adopts conventional method saturation water, mother oil displacement to set up irreducible water saturation; The water drive rock core is set up residual oil saturation; Inject the nutraceutical salt solution that contains microorganism and match then in the rock core with said microorganism;
(2) rock core is sealed at both ends, carries out the microorganism cultivation;
(3) after rock core is cultivated, open the pressure release of rock core port, then with injecting water and nutrient solution displacement rock core;
(4) repeat (2)～(3) step, repeat 10～30 times.
2. method according to claim 1 is characterized in that said microorganism comprises aerobic bacteria, anaerobic bacteria or facultative anaerobe.
3. method according to claim 2, it is characterized in that said microorganism comprises that bacillus subtilis (B.subtilis), bacillus licheniformis (B.licheniformis), happiness heat bites a kind of in oily bacillus (B.thermoleovorans), bacillus stearothermophilus (B.stearothermophilus), pseudomonas aeruginosa (P.aeruginosa), clostridium acetobutylicum (C.acetobutylicum), Leuconostoc mesenteroides (L.mesenteroides), salt bacillus (H.distribution), the Rhodococcus erythropolis (P.erythropolis).
4. method according to claim 1 is characterized in that, said microorganism is that preferred bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa, happiness heat bite oily bacillus and bacillus stearothermophilus.
5. method according to claim 1 is characterized in that, cultivation temperature is microbial growth temperature of being tested or the reservoir temperature that is used for field test.
6. method according to claim 5 is characterized in that, cultivation temperature is 30～90 ℃, and incubation time is 1～10 day.
7. method according to claim 1 is characterized in that, microorganism concn is 10
6～10
7Cells/ml.
8. method according to claim 1 is characterized in that, displacement velocity is 1～10m/d.
9. method according to claim 8 is characterized in that, replaces then with being rich in nutraceutical salt solution displacement with injecting water drive.
10. method according to claim 8 is characterized in that, with being rich in nutraceutical salt solution displacement.
11. according to each described method of claim 1～10, it is characterized in that, (2)～(3) step repeats 10～30 times, then with the identical but core experiment not microbe inoculation of operation as contrast, calculate microbial flooding and improve the oil recovery factor amplitude, with the size of the amplitude that improves oil recovery factor as the parameter index of estimating the microbial oil displacement ability.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102852497A (en) * | 2012-08-07 | 2013-01-02 | 陕西延长石油(集团)有限责任公司研究院 | Compound microorganism oil extraction method for low permeability oilfield |
| CN103104238A (en) * | 2013-01-16 | 2013-05-15 | 中国石油大学(华东) | Microorganism oil displacement numerical simulation method |
| CN104948152A (en) * | 2015-05-29 | 2015-09-30 | 苏州泽方新能源技术有限公司 | Oil field construction technology by means of biological surfactant |
| CN105350947A (en) * | 2015-09-28 | 2016-02-24 | 中国石油化工股份有限公司 | Method for evaluating microorganism oil-displacement field test effects |
| CN111671767A (en) * | 2013-06-05 | 2020-09-18 | 特里赛达公司 | Proton-binding polymers for oral administration |
| CN114427403A (en) * | 2020-09-23 | 2022-05-03 | 中国石油化工股份有限公司 | Thermal recovery assisted microorganism composite huff and puff method and application |
-
2003
- 2003-10-27 CN CNA2003101082034A patent/CN1540136A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102852497A (en) * | 2012-08-07 | 2013-01-02 | 陕西延长石油(集团)有限责任公司研究院 | Compound microorganism oil extraction method for low permeability oilfield |
| CN102852497B (en) * | 2012-08-07 | 2015-09-30 | 陕西延长石油(集团)有限责任公司研究院 | A kind of compound microorganism oil extraction method for low permeability oilfield |
| CN103104238A (en) * | 2013-01-16 | 2013-05-15 | 中国石油大学(华东) | Microorganism oil displacement numerical simulation method |
| CN103104238B (en) * | 2013-01-16 | 2015-08-05 | 中国石油大学(华东) | A kind of microorganism oil displacement numerical simulation method |
| CN111671767A (en) * | 2013-06-05 | 2020-09-18 | 特里赛达公司 | Proton-binding polymers for oral administration |
| CN111671767B (en) * | 2013-06-05 | 2024-03-08 | 特里赛达公司 | Proton-binding polymers for oral administration |
| CN104948152A (en) * | 2015-05-29 | 2015-09-30 | 苏州泽方新能源技术有限公司 | Oil field construction technology by means of biological surfactant |
| CN105350947A (en) * | 2015-09-28 | 2016-02-24 | 中国石油化工股份有限公司 | Method for evaluating microorganism oil-displacement field test effects |
| CN105350947B (en) * | 2015-09-28 | 2017-10-03 | 中国石油化工股份有限公司 | A kind of evaluation method of microbial oil displacement field test results |
| CN114427403A (en) * | 2020-09-23 | 2022-05-03 | 中国石油化工股份有限公司 | Thermal recovery assisted microorganism composite huff and puff method and application |
| CN114427403B (en) * | 2020-09-23 | 2024-04-16 | 中国石油化工股份有限公司 | Method for compositely huff and puff by thermal recovery auxiliary microorganism and application |
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