CN118146779A - Composite yield increasing agent for oil extraction - Google Patents
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- CN118146779A CN118146779A CN202410551627.XA CN202410551627A CN118146779A CN 118146779 A CN118146779 A CN 118146779A CN 202410551627 A CN202410551627 A CN 202410551627A CN 118146779 A CN118146779 A CN 118146779A
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- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims abstract description 9
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 10
- 238000011084 recovery Methods 0.000 claims description 10
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- 235000019270 ammonium chloride Nutrition 0.000 claims description 7
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- 239000012138 yeast extract Substances 0.000 claims description 7
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- DZSVIVLGBJKQAP-UHFFFAOYSA-N 1-(2-methyl-5-propan-2-ylcyclohex-2-en-1-yl)propan-1-one Chemical compound CCC(=O)C1CC(C(C)C)CC=C1C DZSVIVLGBJKQAP-UHFFFAOYSA-N 0.000 claims description 5
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 5
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 4
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 4
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 4
- -1 polyoxyethylene nonylphenol Polymers 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000136 polysorbate Polymers 0.000 claims description 3
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- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 claims description 2
- HWCHICTXVOMIIF-UHFFFAOYSA-M sodium;3-(dodecylamino)propanoate Chemical compound [Na+].CCCCCCCCCCCCNCCC([O-])=O HWCHICTXVOMIIF-UHFFFAOYSA-M 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 claims 1
- 239000003921 oil Substances 0.000 abstract description 42
- 239000010779 crude oil Substances 0.000 abstract description 18
- 238000006073 displacement reaction Methods 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 5
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
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- IGFHQQFPSIBGKE-UHFFFAOYSA-N Nonylphenol Natural products CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 description 1
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- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 description 1
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- 238000012353 t test Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
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- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a compound yield increasing agent for oil extraction. The composite material comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 20-30 parts of a composite microbial inoculum and 30-50 parts of a nutritional agent; the component B consists of the following components in parts by weight: 25-55 parts of biological surfactant, 5-10 parts of organic solvent, 10-20 parts of chemical surfactant and 12-22 parts of emulsifier. The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A is microbial oil extraction, the component B is chemical oil displacement oil extraction, and the two means are combined for use, so that the yield increasing efficiency is higher. The component A is selected from rhodococcus equi and Brevibacillus borsteense composite bacteria, so that the interfacial tension of crude oil is reduced, and the component B adopts sophorolipid and rhamnolipid composite biosurfactant, so that the harvesting efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of oil extraction in an oil field, and particularly relates to a compound yield increasing agent for oil extraction.
Background
Petroleum is an important energy source and chemical raw material, and along with the high-speed development of economy in China, the supply and demand contradiction of petroleum is continuously aggravated due to the relatively poor petroleum resources. At present, most of oil fields in China are positioned in areas with high soil salinization and formation water mineralization, and the application of general chemical oil displacement is greatly limited due to the high mineralization.
With the exploitation of oil reservoirs, a large number of oil wells enter a high water-containing period after being driven by water, an oil-water channel is relatively stable, and a large amount of residual oil is difficult to drive, so that the difficulty of stable production and yield increase of the oil wells is high, and further improvement of the recovery ratio faces serious challenges. Therefore, the development of innovative petroleum yield increasing and recovery ratio improving methods has very important strategic significance.
Microbial oil recovery (MEOR) is a tertiary oil recovery method that utilizes activities such as growth and metabolism of microorganisms in petroleum reservoirs to change reservoir conditions and crude oil properties to enhance crude oil recovery. Microbial oil recovery involves a complex series of changes in biology, physics, chemistry, etc., and can increase crude oil recovery by changing the physical properties of the crude oil or changing the oil displacement environment. The growth and metabolism of the oil extraction microorganism can have certain influence on the components, properties and oil displacement environment of the crude oil, and the metabolic products of the microorganism can emulsify the crude oil, increase the fluidity of the crude oil, increase the formation pressure and change the porosity of the rock, so that the recovery ratio of the crude oil is improved. The oil displacement effect of the existing microbial oil extraction technology is not obvious, and strain improvement or combination with other means is needed.
Disclosure of Invention
The invention aims to provide a compound yield increasing agent for oil extraction.
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 20-30 parts of a composite microbial inoculum and 30-50 parts of a nutritional agent; the component B consists of the following components in parts by weight: 25-55 parts of biological surfactant, 5-10 parts of organic solvent, 10-20 parts of chemical surfactant and 12-22 parts of emulsifier.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus bordetention, and the content of the composite microbial inoculum is 10 7-109 cfu/g.
The preservation number of rhodococcus equi: CGMCC No.3885 purchased from China general microbiological culture Collection center (China Committee for culture Collection of microorganisms); deposit number of the Brevibacillus borstele: CGMCC No.2441, purchased from China general microbiological culture Collection center (China Committee for culture Collection of microorganisms).
The biosurfactant is sophorolipid and rhamnolipid according to the mass ratio of 1:1.
The sophorolipid and rhamnolipid can be obtained by fermentation, can be fermentation liquor extract or concentrate, can be pure products obtained by separation and purification of organic solvents, and can be liquid, semisolid, solid paste or powder. Sophorolipids are also commercially available, CAS 148409-20-5; rhamnolipids are also commercially available, CAS 869062-42-0.
The organic solvent is one or more of propanol, butanol, propylene glycol and acetone.
The nutritional agent comprises the following components in parts by weight: 15-25 parts of yeast extract, 3-8 parts of ammonium chloride and 12-17 parts of dipotassium hydrogen phosphate.
The chemical surfactant is one or more of 2-acrylamide-2-methylpropanesulfonic acid, N-dodecyl dimethyl betaine and N-dodecyl-beta-sodium aminopropionate.
The emulsifier is one or more of Tween 20, tween 40, tween 80 and polyoxyethylene nonylphenol ether.
The component A and the component B are stored separately, and when the composition A is used, 30-50 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 20-40 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
The invention has the beneficial effects that: the compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A is microbial oil extraction, the component B is chemical oil displacement oil extraction, and the two means are combined for use, so that the yield increasing efficiency is higher. The component A is selected from rhodococcus equi and Brevibacillus borsteense composite bacteria, so that the interfacial tension of crude oil is reduced, and the component B adopts sophorolipid and rhamnolipid composite biosurfactant, so that the harvesting efficiency is improved.
Detailed Description
The present invention will be described more fully hereinafter in order to facilitate an understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 25 parts of a composite microbial inoculum and 40 parts of a nutritional agent; the component B consists of the following components in parts by weight: 40 parts of biosurfactant, 8 parts of propanol, 15 parts of 2-acrylamide-2-methylpropanesulfonic acid and 18 parts of tween 20.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus borstevensis, wherein the content of rhodococcus equi in the microbial inoculum is 5X10 7 cfu/g, and the content of Brevibacillus borstevensis in the microbial inoculum is 1X10 8 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
The biosurfactant is sophorolipid and rhamnolipid according to the mass ratio of 1:1.
The nutritional agent comprises the following components in parts by weight: 20 parts of yeast extract, 6 parts of ammonium chloride and 14 parts of dipotassium hydrogen phosphate.
The component A and the component B are stored separately, and when the composition A is used, 40 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 30 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
Example 2
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 20 parts of a composite microbial inoculum and 30 parts of a nutritional agent; the component B consists of the following components in parts by weight: 25 parts of biosurfactant, 5 parts of butanol, 10 parts of N-dodecyl-beta-aminopropionic acid sodium salt and 12 parts of tween 80.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus borstevensis, wherein the content of rhodococcus equi in the microbial inoculum is 2X10 7 cfu/g, and the content of Brevibacillus borstevensis in the microbial inoculum is 6X10 7 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
The biosurfactant is sophorolipid and rhamnolipid according to the mass ratio of 1:1.
The nutritional agent comprises the following components in parts by weight: 15 parts of yeast extract, 3 parts of ammonium chloride and 12 parts of dipotassium hydrogen phosphate.
The component A and the component B are stored separately, and when the composition A is used, 30 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 20 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
Example 3
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 30 parts of a composite microbial inoculum and 50 parts of a nutritional agent; the component B consists of the following components in parts by weight: 55 parts of biosurfactant, 10 parts of acetone, 20 parts of N-dodecyl dimethyl betaine and 22 parts of nonylphenol polyoxyethylene ether.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus bordetention, wherein the content of rhodococcus equi in the microbial inoculum is 8X10 7 cfu/g, and the content of Brevibacillus bordetention in the microbial inoculum is 2X10 8 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
The biosurfactant is sophorolipid and rhamnolipid according to the mass ratio of 1:1.
The nutritional agent comprises the following components in parts by weight: 25 parts of yeast extract, 8 parts of ammonium chloride and 17 parts of dipotassium hydrogen phosphate.
The component A and the component B are stored separately, and when the composition A is used, 50 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 40 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
Comparative example 1
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 25 parts of a composite microbial inoculum and 40 parts of a nutritional agent; the component B consists of the following components in parts by weight: 40 parts of sophorolipid, 8 parts of propanol, 15 parts of 2-acrylamide-2-methylpropanesulfonic acid and 18 parts of tween.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus borstevensis, wherein the content of rhodococcus equi in the microbial inoculum is 5X10 7 cfu/g, and the content of Brevibacillus borstevensis in the microbial inoculum is 1X10 8 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
The nutritional agent comprises the following components in parts by weight: 20 parts of yeast extract, 6 parts of ammonium chloride and 14 parts of dipotassium hydrogen phosphate.
The component A and the component B are stored separately, and when the composition A is used, 40 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 30 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
Comparative example 2
The compound yield increasing agent for oil extraction comprises a component A and a component B, wherein the component A consists of the following components in parts by weight: 25 parts of a composite microbial inoculum and 40 parts of a nutritional agent; the component B consists of the following components in parts by weight: 40 parts of rhamnolipid, 8 parts of propanol, 15 parts of 2-acrylamide-2-methylpropanesulfonic acid and 18 parts of tween 20.
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus borstevensis, wherein the content of rhodococcus equi in the microbial inoculum is 5X10 7 cfu/g, and the content of Brevibacillus borstevensis in the microbial inoculum is 1X10 8 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
The nutritional agent comprises the following components in parts by weight: 20 parts of yeast extract, 6 parts of ammonium chloride and 14 parts of dipotassium hydrogen phosphate.
The component A and the component B are stored separately, and when the composition A is used, 40 times of water in parts by weight is added into the composition A, and the composition A and the composition B are uniformly mixed; adding 30 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
Experimental example:
Preparation of rhodococcus equi crude oil fermentation liquor: rhodococcus equi (viable bacteria content 1X10 8 cfu/g) was inoculated at 2% of the mass of the medium, which was formulated as follows: NH 4Cl 3g/L,K2HPO4 2g/L,KH2PO4 3g/L,CaCl2 0.3.3 g/L and crude oil 2.5g/L. The culture temperature is 42 ℃, the culture time is 5 days, and the rotation speed of a shaking table is 180rpm, so that fermentation broth is obtained.
Preparation of Brevibacillus borstele crude oil fermentation broth: brevibacillus bordetensis (viable bacteria content 1X10 8 cfu/g) was inoculated at 2% of the mass of the medium, the medium formulation was: NH 4Cl 3g/L,K2HPO4 2g/L,KH2PO4 3g/L,CaCl2 0.3.3 g/L and crude oil 2.5g/L. The culture temperature is 42 ℃, the culture time is 5 days, and the rotation speed of a shaking table is 180rpm, so that fermentation broth is obtained.
Preparation of rhodococcus equi, brevibacillus borstele crude oil fermentation broth: rhodococcus equi (live bacteria content 1X10 8 cfu/g) and Brevibacillus borstele (live bacteria content 1X10 8 cfu/g) are respectively inoculated according to 1% of the mass of a culture medium, wherein the formula of the culture medium is as follows: NH 4Cl 3g/L,K2HPO4 2g/L,KH2PO4 3g/L,CaCl2 0.3.3 g/L and crude oil 2.5g/L. The culture temperature is 42 ℃, the culture time is 5 days, and the rotation speed of a shaking table is 180rpm, so that fermentation broth is obtained.
Adopting LAUDA TVT liquid drop volume tensiometer to measure interfacial tension of liquid before and after fermentation, calculating reduction rate of interfacial tension, and the calculation formula is: (Medium interfacial tension-fermentation broth interfacial tension)/Medium interfacial tension X100%
Each group of experiments was averaged 3 times and statistically analyzed using SPSS 24.0 software with the data results measuredThe (mean ± standard deviation) represents that the data normalization test is carried out by adopting a Kolmogorov-Smirnov test method, and the comparison of the mean difference between two groups adopts a t test for data conforming to the normal distribution, and has statistical significance by taking P <0.05 as the difference.
The measurement results are shown in Table 1:
TABLE 1
Note that: * Represents P <0.05 compared to Rhodococcus equi and Brevibacillus bordetention fermentation groups.
Selecting a crude oil-containing porous medium with 20% of porosity and 110 mD% of water permeability, diluting the B component in examples 1-3 and comparative examples 1-2 by 30 times, injecting the diluted B component into the oil-containing porous medium at a flow rate of 0.04 cm/min for displacement, wherein the total injection amount is 1.5 times of pore volume, and the oil flows out of the porous medium along with the B component diluent in the displacement process; collecting effluent liquid, recovering oil phase after oil-water delamination, measuring recovery ratio, and calculating the formula: the volume of the recovered oil phase/volume of crude oil X100%.
The measurement results are shown in Table 2:
TABLE 2
Note that: * Representing a comparison of P <0.05 with example 1 group.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (7)
1. The compound yield increasing agent for oil extraction is characterized by comprising a component A and a component B, wherein the component A consists of the following components in parts by weight: 20-30 parts of a composite microbial inoculum and 30-50 parts of a nutritional agent; the component B consists of the following components in parts by weight: 25-55 parts of biological surfactant, 5-10 parts of organic solvent, 10-20 parts of chemical surfactant and 12-22 parts of emulsifier;
The composite microbial inoculum comprises rhodococcus equi and Brevibacillus bordetention, wherein the content of the composite microbial inoculum is 10 7-109 cfu/g; the preservation number of rhodococcus equi: CGMCC No.3885; deposit number of the Brevibacillus borstele: CGMCC No.2441.
2. The compound yield increasing agent for oil extraction according to claim 1, wherein the biosurfactant is sophorolipid and rhamnolipid according to a mass ratio of 1: 1.
3. The compound stimulation agent for oil recovery according to claim 1, wherein the organic solvent is one or more of propanol, butanol, propylene glycol and acetone.
4. The compound yield increasing agent for oil extraction according to claim 1, wherein the nutritional agent comprises the following components in parts by weight: 15-25 parts of yeast extract, 3-8 parts of ammonium chloride and 12-17 parts of dipotassium hydrogen phosphate.
5. The compound yield increasing agent for oil extraction according to claim 1, wherein the chemical surfactant is one or more of 2-acrylamide-2-methylpropanesulfonic acid, N-dodecyl dimethyl betaine and sodium N-dodecyl-beta-aminopropionate.
6. The compound yield increasing agent for oil extraction according to claim 1, wherein the emulsifier is one or more of tween 20, tween 40, tween 80 and polyoxyethylene nonylphenol ether.
7. The compound yield increasing agent for oil extraction according to claim 1, wherein the component A and the component B are stored separately, and when in use, the component A is added with 30-50 times of water by weight and uniformly mixed; adding 20-40 times of water in parts by weight into the component B, and uniformly mixing; and respectively injecting oil layers.
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