CN102732424A - Compounded oil extraction microbe and its application in thickened oil and extra thickened oil exploitation - Google Patents
Compounded oil extraction microbe and its application in thickened oil and extra thickened oil exploitation Download PDFInfo
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Abstract
The invention relates to a new method to increase the recovery efficiency of thickened oil and extra thickened oil by using compounded oil extraction microbe, specifically speaking, the exploitation objects are the thickened oil with ground viscosity of greater than or equal to 5000 mPa.s and the extra thickened oil with ground viscosity of greater than or equal to 50000 mPa.s. The microbe with the compounded function is the mixed strain with special functions which is capable of substantially reducing the viscosity of the thickened oil and extra thickened oil and is selected from a laboratory. The compounded microbe, and an abundant carbon source-containing nutrient solution and an optimization agent are injected to an oil reservoir by a specific technology, the crude oil viscosity can be reduced by 50-95% by the integration effect of various active substances generated in the microbe metabolism process, thereby the fluidity of the crude oil can be increased to reach the purpose of raising the recovery efficiency of thickened oil and extra thickened oil.
Description
Technical field
The invention belongs to microbial enhanced oil recovery technical field (MEOR; Microbial Enhanced Oil Recovery), relates generally to a kind of method of utilizing mikrobe to exploit special viscous crude, more specifically say so to viscous crude and the special heavy crude reservoir of viscosity of crude >=5000mPas; Oil reservoir is injected in specified microorganisms, nutritive medium and optimization agent; Utilize the metabolic processes of bacterial strain to make viscosity of crude reduce, improve crude oil fluidity, thereby increase the technology of output.
Background technology
Viscous crude is the valuable source of development of world economy, and its reserves have 4000～6000 billion cubic meters approximately.Also there is abundant viscous crude resource in China, according to incompletely statistics, verifies with controlled reserve and has reached 1,600,000,000 tons, and emphasis is distributed in oil fields such as triumph, the Liaohe River, Henan, Xinjiang.In the about 10,000,000,000,000 barrels of residual petroleum resources in the whole world, more than 70% the viscous crude resource.The Chinese government clearly proposes to carry out energetically the exploratory development of unconventional petroleum resourceses such as resinous shale, oil-sand, gas hydrate in " 12 " development program, increase spending on science and technology, and reduces cost of winning, increases the degree of protection of China's hydrocarbon resources.
China just has the research and development to viscous crude in mind the eighties in last century, presses the characteristics of heavy crude reservoir, and also each is different to some extent for its mining type, but is viscosity reduction basically and the direction that molecule is diminished lighten.At present, improve the most successful reoovery method of RF and divide two big types: the one, inject fluid thermal recovery or displacement type method, like hot waterflooding, steam stimulation, steam flood, fireflood etc.; Another kind of is the Yield increasing type mining type, comprises horizontal well, compound Multilateral Wells, waterfrac treatment, electrically heated, chemical viscosity reduction etc., and being used in combination of these two types of technology become the main means of current heavy oil development.Wherein, Shengli Oil Field adopts RF that thermal recovery, steam treatment, electricity heating, chemical viscosity reduction (polymer injection drives) technology of etc.ing improve viscous crude with maintenance output.
Got into since the nineties, accelerated the research steps of microbial enhanced oil recovery technology (MEOR), the bacterial classification Study on compatibility that Daqing oil field is carried out, progress makes a breakthrough.In recent years, successively introduced microniological proudcts, accelerated the development of China MEOR from many aspects from the U.S., Canada.In Xinjiang, oil fields such as grand celebration, Fuyu County, huge port, triumph, Ji Dong, the Liaohe River, Jiang-Han Area have carried out applying of microbe oil production technology, guiding test is about 2000 wells altogether.Field test by individual well to the block integral developing test; Develop to mid-deep strata by shallow-layer; Have high seepage well in, the development of low seepage well; Low hot-well is arranged to hot hole development (top temperature is 102 ℃); Low water cut well is arranged to high water-cut well development (the highest moisture 80%); Develop (the longest C by long-chain in the crude oil N-alkanes to long-chain
60); Develop (minimum only have 5%) by the higher volatile component of crude oil to low volatile component; Wax content is the highest by 51.4%; Bituminous matter, gum level are the highest by 31.6%; Ordinary test well viscosity of crude is many at 9.33～9.87mPas, and zero pour is many more than 46～47 ℃.Index that guiding test oil field reaches and effect are that crude oil water content generally descends more than 5%, and 60%～70% processing well obtains oil increasing effect (being up to 84%); Oil well output generally increases by 46%～68% (being up to 104%); CO
2Content increases by 21%, obvious economic, 1: 4～1: 6 (being up to 1: 9) of input-output ratio.8～12 months (being up to 18 months) of best validity period of raising the output.The joint study pattern has had the new beginning; The Chinese Academy of Sciences, Nankai University and how tame scientific research institution of Kelamayi Petroleum Chemical Engineering Institute have participated in the joint study work relevant with the oil field, for shop experiment changes the process that field test provides solid theory and advanced the achievement industrialization as early as possible over to.
Summary of the invention
The present invention provides a kind of composite oil extraction microbial and is used to develop the new technology of viscous crude and special heavy crude reservoir.
Its technical scheme is:
A kind of composite oil extraction microbial is composite viscosity reduction mikrobe, and said mikrobe is composite microbiobacterial agent, presses dry weight basis, fills a prescription to be: Rhodococcus equi BS001,30%～40%; Subtilis BS002,60%～70%.The BS001 preserving number is CGMCC No.3885, preservation date be on June 1st, 2010 (Mei Xiaodan, Fu Bufei, Liu Ying etc., a kind of composite fungus agent and processing return row fracturing liquid be the biological method of displacement of reservoir oil active water, number of patent application: 201010215973.9); The BIT09S1 preserving number is CGMCC No.2947, and preservation date is that on March 11st, 2009, (preparation method of subtilis and lipopeptid type biological surfactant, number of patent application was: 200910300898.3).Depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center.
Utilize composite oil extraction microbial to come the method for the special viscous crude of heavy crude producing: utilize composite oil extraction microbial fermented liquid, nutritive medium and optimize agent, with the technology mode injection oil well or the well of single well stimulation or water filling, closing well recovers normal exploitation after 3～30 days;
Said nutritive medium is: contain potassium primary phosphate in every cubic metre of water, 2.5～4.5kg; Sodium phosphate, dibasic, 0.7～1.4kg; Ammonium chloride, 0.5～0.9kg; Configuration nutritive medium institute water is that field joint stations is handled back re-injection water, and temperature is between 20～65 ℃, and the pH value is between 6.0～9.0.
Said optimization agent is that Institute of Micro-biology produces glycolipid class tensio-active agent, makes after concentrating through purifying.Concrete grammar: the centrifugal 20min of fermented liquid 8000rpm is removed thalline, and the supernatant lyophilize gets powdery substance, uses dissolve with methanol, the centrifugal decon of 10000rpm, and supernatant steams in 50 ℃ of underspins, gets soup compound, with the NaHCO of 0.05M
3Dissolve it, transfer pH to 2.0 with concentrated hydrochloric acid, 4 ℃ are spent the night, and the 10000rpm centrifugal collecting precipitation is the optimization agent.
The acquisition process of said microbial fermentation solution is that aerobic three grade fermemtation is adopted in the fermentation of said mikrobe:
1. one grade fermemtation: the configuration minimal medium, its prescription is: glucose, 6～10g/L; Ammonium chloride, 0.1～0.3g/L; Potassium primary phosphate, 2.5～4.0g/L; Sodium phosphate, dibasic, 1.0～1.8g/L, surplus is a water, adjusts its pH in 7.0～7.5 scopes with hydrochloric acid or sodium hydroxide; Insert composite microbial inoculum by 5% volume ratio, 35～38 ℃, shaking table is cultivated 18～24h under the 150rpm rotating speed; Above material purity is analytical pure.
2. second order fermentation: in the 250L seeding tank, add the 200L fermentation raw material, its prescription is: molasses, 2～5kg; Potassium primary phosphate, 0.5～0.8kg; Sodium phosphate, dibasic, 0.25～0.45kg; Ammonium chloride, 0.4～0.6kg; Deionized water, 200L; Behind 125 ℃ of sterilization 20min by volume inoculum size 5% insert step 1. in one grade fermemtation liquid; 5～40 ℃, 40～60rpm rotating speed bottom fermentation, 18～20h; Wherein potassium primary phosphate, Sodium phosphate, dibasic and purity of ammonia chloride are technical grade, and the molasses sugar degree is more than 45%.
3. three grade fermemtation: in the 1000L fermentor tank, add the 800L fermentation raw material, its prescription is: molasses, 10～20kg; Potassium primary phosphate, 2.0～4.0kg; Sodium phosphate, dibasic, 1.0～2.0kg; Ammonium chloride, 2.0～3.0kg; Deionized water, 1000L; Behind 125 ℃ of sterilization 20min by volume inoculum size 5% insert step 2. in second order fermentation liquid; 35～40 ℃, 30～50rpm rotating speed bottom fermentation, 20～24h; Get microbial fermentation solution, in the 200kg plastic tank, deposit.Wherein potassium primary phosphate, Sodium phosphate, dibasic and purity of ammonia chloride are technical grade, and the molasses sugar degree is more than 45%.
The configuration of construction raw material:
1. the configuration of nutritive medium: site operation for ease, nutritive medium directly disposes in tank truck.Adopt add earlier add water behind the raw material way so that the fully dissolving in water of various raw material.Per car adds: potassium primary phosphate, 30～50kg; Sodium phosphate, dibasic, 15～25kg; Ammonium chloride, 20～25kg.Wherein potassium primary phosphate, Sodium phosphate, dibasic and purity of ammonia chloride are technical grade.Go to joint station water intaking 10～15m after tank truck is reinforced
3After directly go to the working-yard.
2. the configuration of molasses water solution: site operation for ease, molasses water solution also directly disposes in tank truck.Per car adds molasses 1～1.5t.Molasses hammer degree >=45%.Go to joint station water intaking 10～15m after tank truck is reinforced
3After directly go to the working-yard.
3. optimize the agent configuration:, must will optimize agent and add in the entry owing to optimize the low surface tension of agent.Tank truck goes to joint station water intaking 10～15m
3After directly go to the working-yard.Per car adds optimizes agent 10～30kg.
The on-the-spot injection, the construction idiographic flow is following:
1. inject the microbial fermentation solution diluent: the tank truck in that nutritive medium is housed adds microbial fermentation solution, and per car adds 1.0～1.3t, adds the back and injects oil well.
2. implantation quality concentration is 10% molasses water solution: directly inject;
3. inject the microbial fermentation solution diluent; The 1. middle microbial fermentation solution of implantation step;
4. inject nutritive medium: directly inject;
5. inject and optimize dilution agent liquid, directly inject;
6. inject 5～10m
3Water;
7. closing well is 3～10 days.
Microbial fermentation solution dilutes with nutritive medium, and mass concentration is 5%～9%; Optimizing the agent mass concentration is 0.1%～0.3%.Injection mode is the oil well casing annular space and injects.
Said single well stimulation technological process is that mikrobe, nutritive medium and optimization agent alternately mix injects; Concrete injection is in proper order:
Microbial fermentation solution diluent → molasses diluent → microbial fermentation solution diluent → nutritive medium → optimization dilution agent liquid → displacing liquid → closing well
Individual-well injection rate according to the oil reservoir characteristic at 50～1000m
3Scope: with the then corresponding adjustment of lower volume
1. inject the microbial fermentation solution diluent, volume is at 10～200m
3In the scope;
2. inject molasses water solution, volume is at 10～200m
3In the scope;
3. inject the microbial fermentation solution diluent, volume is at 10～200m
3Between;
4. inject nutritive medium, volume is at 10～200m
3Between;
5. inject and optimize dilution agent liquid, volume is at 5～100m
3Between;
6. inject displacing liquid 5～100m
3Water;
7. closing well is 3～30 days.
Microbial fermentation solution dilutes with nutritive medium, and mass concentration is 5%～9%; Optimize agent and molasses with the dilution of re-injection water, optimizing the agent mass concentration is 0.1%～0.3%, and the molasses mass concentration is 10%; Injection mode is the oil well casing annular space and injects.
This technology has that zymotechnique is simple, with low cost, the quality guaranteed period length, raw material convenient transportation, site operation is flexible, use range is extensive, simple to operate, effect of increasing production obviously, the input-output ratio advantages of higher.Be particularly suitable for large-scale industrial production.
Description of drawings
Day output graphic representation before and after 1 construction of Fig. 1 test pit;
Day output graphic representation before and after 2 constructions of Fig. 2 test pit.
Embodiment
Embodiment 1
The composite viscosity reduction mikrobe BS001 and the preparation of BIT09S1 mixed fermentation liquid realize in the following manner among the present invention:
1. one grade fermemtation: use deionized water configuration minimal medium, its prescription is: glucose, 10g/L; Ammonium chloride, 0.3g/L; Potassium primary phosphate, 4.0g/L; Sodium phosphate, dibasic, 1.8g/L is with hydrochloric acid or its pH to 7.2 of sodium hydroxide adjustment.Insert composite microbial inoculum by 5% volume ratio, 38 ℃, shaking table is cultivated 18h under the 150rpm rotating speed.
2. second order fermentation: in the 250L seeding tank, add the 200L fermentation raw material, its prescription is: molasses, 5kg; Potassium primary phosphate, 0.8kg; Sodium phosphate, dibasic, 0.45kg; Ammonium chloride, 0.5kg; Deionized water, 200L.Insert one grade fermemtation liquid in the step 1 by inoculum size 5% behind 125 ℃ of sterilization 20min.40 ℃, 50rpm rotating speed bottom fermentation 20h.
3. three grade fermemtation: in the 1000L fermentor tank, add the 800L fermentation raw material, its prescription is: molasses, 20kg; Potassium primary phosphate, 4.0kg; Sodium phosphate, dibasic, 2.0kg; Ammonium chloride, 3.0kg; Deionized water, 1000L.Insert second order fermentation liquid in the step 2 by inoculum size 5% behind 125 ℃ of sterilization 20min.40 ℃, 30rpm rotating speed bottom fermentation 24h gets microbial fermentation solution, in the 200kg plastic tank, deposits.
Wherein the various raw materials of one grade fermemtation use are analytical pure, and the various material purities that second order fermentation, three grade fermemtation use are technical grade.
The preparation of various construction raw materials realizes in the following manner among the present invention:
1. the configuration of nutritive medium adds in every tank truck: potassium primary phosphate, 50kg; Sodium phosphate, dibasic, 25kg; Ammonium chloride, 25kg.Wherein potassium primary phosphate, Sodium phosphate, dibasic and purity of ammonia chloride are technical grade.Go to joint station water intaking 15m after tank truck is reinforced
3
2. the configuration of molasses water solution adds molasses 1～1.5t in every tank truck.The molasses sugar degree is more than 45%.Go to joint station water intaking 15m after tank truck is reinforced
3
3. optimize the configuration of dilution agent liquid, tank truck goes to joint station water intaking 15m
3Back per car adds optimizes agent 20kg.
Improving special heavy oil block RF among the present invention realizes in the following manner: test pit 1 is positioned at the special heavy oil block in Liaohe Oil Field, more than 800 meter of reservoir buried depth, and rate of permeation is 0.7-2.6 μ m
2, porosity is 25-30%, viscosity of crude 200-100000mpas, oil density average out to 0.9-1.0g/cm
3, gum asphaltic content reaches more than 30%.
The dilution of microbial fermentation solution: in the tank truck that nutritive medium 13.7t is housed, add microbial fermentation solution, per car adds 1.3t, and the appearance of vehicle amount is 15t.Injection technology:
1, inject the microbial fermentation solution diluent: casing annulus injects, and IR is 3 cars;
2, implantation quality concentration is 10% molasses water solution: casing annulus injects, and IR is 1 car;
3, inject the microbial fermentation solution diluent: casing annulus injects, and IR is 1 car;
4, inject nutritive medium: casing annulus injects, and IR is 1 car;
5, inject optimization dilution agent liquid, casing annulus injects, and IR is 1 car;
6, inject 5m
3Water;
7, closing well is 10 days.
Its construction results is as shown in Figure 1.
Use six rotating speed viscometer determinings construction front and back viscosity of crude, the result is following:
Shown by above-mentioned single well stimulation experimental data: about the average 0.4t of daily oil production, construction back day output on average reaches 2.5t before this well construction.Back second monthly output of wherein constructing reaches peak value, and day produce oil is at 4～5t.This well stable yields 3 months, accumulative total increases oil and reaches 148.3t.The double of output shows that this invention oil increasing effect is very obvious.
Improving the special heavy oil block RF of viscous crude among the present invention realizes in the following manner: test pit 2 is positioned at the special heavy oil block in Liaohe Oil Field, more than 800 meter of reservoir buried depth, and rate of permeation is 0.7-2.6 μ m
2, porosity is 25-30%, viscosity of crude 200-100000mpas, oil density average out to 0.9-1.0g/cm
3, gum asphaltic content reaches more than 30%.
The dilution of microbial fermentation solution: in the tank truck that nutritive medium 13.7t is housed, add microbial fermentation solution, per car adds 1.3t, and the appearance of vehicle amount is 15t.Injection technology:
1, inject the microbial fermentation solution diluent: casing annulus injects, and IR is 3 cars;
2, implantation quality concentration is 10% molasses water solution: casing annulus injects, and IR is 1 car;
3, inject the microbial fermentation solution diluent: casing annulus injects, and IR is 1 car;
4, inject nutritive medium: casing annulus injects, and IR is 1 car;
5, inject optimization dilution agent liquid, casing annulus injects, and IR is 1 car;
6, inject 5m
3Water;
7, closing well is 10 days.
Its construction results is as shown in Figure 2.
Use six rotating speed viscometer determinings construction front and back viscosity of crude, the result is following:
Shown by above-mentioned single well stimulation experimental data: about the average 0.6t of daily oil production, construction back day output on average reaches 3.1t before this well construction.Wherein construction the 3rd monthly output in back reaches peak value, day produce oil 5～6t.This well stable yields 4 months, accumulative total increases oil and reaches 158.3t.The double of output shows that this invention oil increasing effect is very obvious.
This composite functional microorganism is to the specific function hybrid bacterial strain that can significantly reduce viscous crude and special viscosity of thickened oil in laboratory screening.With composite mikrobe and contain the nutritive medium that enriches carbon source and optimize agent and inject oil reservoir through special process; Comprehensive action through the various active material that produces in the mikrobe metabolic processes can make viscosity of crude reduce 50-95%, and the flowability that increases crude oil is to reach the purpose that improves viscous crude and special heavy oil block RF.
Claims (6)
1. composite oil extraction microbial, it is characterized in that: said mikrobe is composite microbiobacterial agent, presses dry weight basis, filling a prescription is: Rhodococcus equi BS001,30% ~ 40%; Subtilis BIT09S1,60% ~ 70%;
The BS001 preserving number is CGMCC No.3885, and preservation date is on June 1st, 2010; The BIT09S1 preserving number is CGMCC No.2947, and preservation date is on March 11st, 2009; Depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center.
2. one kind is utilized the application of the composite oil extraction microbial of claim 1 in viscous crude and special viscous oil recovery; It is characterized in that: utilize composite oil extraction microbial fermented liquid, nutritive medium and optimize agent; Technology mode with single well stimulation or water filling injects oil well or well, and closing well recovers normal exploitation after 3 ~ 30 days;
Said nutritive medium is: contain potassium primary phosphate in every cubic metre of water, 2.5 ~ 4.5 kg; Sodium phosphate, dibasic, 0.7 ~ 1.4 kg; Ammonium chloride, 0.5 ~ 0.9 kg;
Said optimization agent is produced glycolipid class table agent bullion alive by bacterial strain BS001.
3. application according to claim 2 is characterized in that: configuration nutritive medium institute water is that field joint stations is handled back re-injection water, and temperature is between 20 ~ 65 ℃, and the pH value is between 6.0 ~ 9.0.
4. application according to claim 2 is characterized in that: said optimization agent is that Institute of Micro-biology produces glycolipid class tensio-active agent, makes after concentrating through purifying; Specific practice is: centrifugal 20 min of bacterial strain BS001 fermented liquid 8000 rpm are removed thalline; The supernatant lyophilize gets powdery substance, uses dissolve with methanol, the centrifugal decon of 10000 rpm; Supernatant steams in 50 ℃ of underspins, gets soup compound, with the NaHCO of 0.05M
3Dissolve it; Transfer pH to 2.0 with concentrated hydrochloric acid, 4 ℃ are spent the night, and 10000 rpm centrifugal collecting precipitations are the optimization agent.
5. application according to claim 2 is characterized in that: the acquisition process of said microbial fermentation solution is aerobic three grade fermemtation:
1. one grade fermemtation: the configuration minimal medium, its prescription is: glucose, 6 ~ 10 g/L; Ammonium chloride, 0.1 ~ 0.3 g/L; Potassium primary phosphate, 2.5 ~ 4.0 g/L; Sodium phosphate, dibasic, 1.0 ~ 1.8 g/L, surplus is a water, adjusts its pH in 7.0 ~ 7.5 scopes with hydrochloric acid or sodium hydroxide; Insert composite microbial inoculum by 5% volume ratio, 35 ~ 38 ℃, shaking table is cultivated 18 ~ 24 h under the 150 rpm rotating speeds;
2. second order fermentation: in 250 L seeding tanks, add 200 L fermentation raw materials, its prescription is: molasses, 2 ~ 5 kg; Potassium primary phosphate, 0.5 ~ 0.8 kg; Sodium phosphate, dibasic, 0.25 ~ 0.45 kg; Ammonium chloride, 0.4 ~ 0.6 kg; Deionized water, 200 L; Behind 125 ℃ of sterilization 20 min by volume inoculum size 5% insert step 1. in one grade fermemtation liquid; 5 ~ 40 ℃, 40 ~ 60 rpm rotating speed bottom fermentations, 18 ~ 20 h;
3. three grade fermemtation: in 1000 L fermentor tanks, add 800 L fermentation raw materials, its prescription is: molasses, 10 ~ 20 kg; Potassium primary phosphate, 2.0 ~ 4.0 kg; Sodium phosphate, dibasic, 1.0 ~ 2.0 kg; Ammonium chloride, 2.0 ~ 3.0 kg; Deionized water, 1000 L; Behind 125 ℃ of sterilization 20 min by volume inoculum size 5% insert step 2. in second order fermentation liquid; 35 ~ 40 ℃, 30 ~ 50 rpm rotating speed bottom fermentations, 20 ~ 24 h; Get microbial fermentation solution.
6. application according to claim 2 is characterized in that:
Said single well stimulation technological process is that mikrobe, nutritive medium and optimization agent alternately mix injects; Concrete injection is in proper order:
Individual-well injection rate according to the oil reservoir characteristic at 50 ~ 1000 m
3Scope: with the then corresponding adjustment of lower volume
1. inject the microbial fermentation solution diluent, volume is at 10 ~ 200 m
3In the scope;
2. inject molasses water solution, volume is at 10 ~ 200 m
3In the scope;
3. inject the microbial fermentation solution diluent, volume is at 10 ~ 200 m
3Between;
4. inject nutritive medium, volume is at 10 ~ 200 m
3Between;
5. inject and optimize dilution agent liquid, volume is at 5 ~ 100 m
3Between;
6. inject displacing liquid 5 ~ 100 m
3Water;
7. closing well is 3 ~ 30 days;
Microbial fermentation solution dilutes with nutritive medium, and mass concentration is 5% ~ 9%; Optimize agent and molasses with the dilution of re-injection water, optimizing the agent mass concentration is 0.1% ~ 0.3%, and the molasses mass concentration is 10%; Injection mode is the oil well casing annular space and injects.
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| CN103613635A (en) * | 2013-10-12 | 2014-03-05 | 北京市捷博特能源技术有限公司 | Preparation and application of lipopeptide solution for low porosity and low permeability oil field displacement |
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| CN104329066B (en) * | 2014-09-09 | 2016-09-14 | 中国石油化工股份有限公司 | A kind of microorganism for heavy oil wells and CO2compound single well stimulation oil production method |
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| CN109653719A (en) * | 2018-12-17 | 2019-04-19 | 中国石油大学(北京) | A method of fine and close viscous crude fracturing effect is improved using microorganism in situ |
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| CN103912249A (en) * | 2013-01-09 | 2014-07-09 | 中国石油化工股份有限公司 | Method for conveying microorganisms to stratum |
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