CN108373912B - Salt-resistant high-temperature-resistant foam scrubbing agent and preparation method thereof - Google Patents

Salt-resistant high-temperature-resistant foam scrubbing agent and preparation method thereof Download PDF

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CN108373912B
CN108373912B CN201810108125.4A CN201810108125A CN108373912B CN 108373912 B CN108373912 B CN 108373912B CN 201810108125 A CN201810108125 A CN 201810108125A CN 108373912 B CN108373912 B CN 108373912B
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salt
temperature
culture medium
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CN108373912A (en
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刘岳龙
周舰
王锦昌
张凌筱
赵静
周瑞立
赵润冬
梅洁
袁胥
谭苗
张立强
李璇
陈旭
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Petroleum Engineering Technology Research Institute China Petrochemical Corp North China Oil & Gas Co
China Petroleum and Chemical Corp
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Petroleum Engineering Technology Research Institute China Petrochemical Corp North China Oil & Gas Co
China Petroleum and Chemical Corp
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    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
    • C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
    • C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
    • C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
    • C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants

Abstract

The invention relates to a salt-resistant high-temperature-resistant foam scrubbing agent and a preparation method thereof, belonging to the technical field of oil and gas field development. The salt-resistant high-temperature-resistant foam scrubbing agent disclosed by the invention comprises the following components in percentage by mass: 0.2-0.5% of an anionic-nonionic surfactant composite system, 0.02-0.08% of microbial fermentation liquor and the balance of water; the microbial fermentation liquid is microbial strain fermentation liquid for producing glycolipid biosurfactant or lipopeptide biosurfactant. The salt-resistant high-temperature-resistant foam scrubbing agent disclosed by the invention has the capabilities of salt resistance, high-temperature resistance, foaming stabilization and liquid carrying, and simultaneously has good condensate oil resistance and methanol resistance, and is beneficial to environmental protection and sustainable development of oil and gas fields.

Description

Salt-resistant high-temperature-resistant foam scrubbing agent and preparation method thereof
Technical Field
The invention relates to a salt-resistant high-temperature-resistant foam scrubbing agent and a preparation method thereof, belonging to the technical field of oil and gas field development.
Background
A large number of liquid-gas producing wells in China have the problems of high liquid-gas ratio and difficult liquid drainage, and in order to prevent gas wells from generating hydrates, an underground throttling process is mostly adopted, so that the production pressure is reduced, and the liquid drainage difficulty is further increased.
By using the foam liquid discharge process, various physical-chemical effects such as foam, dispersion, resistance reduction, washing and the like can be generated in the gas-liquid two-phase mixing vertical flow process, the slippage loss in a shaft is reduced, the gas flow vertical liquid lifting capacity is improved, and the aim of improving the drainage and gas production efficiency is fulfilled; in the drainage process technology series, the foam drainage process is convenient to construct, simple in equipment, convenient to construct, low in cost, large in applicable well depth range, free of influence on normal production of gas wells, low in cost and high in efficiency, so that the foam drainage process is generally concerned at home and abroad.
Generally speaking, whether foam drainage is successfully implemented or not and whether drainage and gas recovery efficiency can be effectively improved or not depends on the performance of the used foam drainage agent to a great extent, so that the optimal selection and the standard improvement modification of the foam drainage agent are important in the research of foam drainage technology.
For improving the temperature resistance and salt tolerance, methanol resistance and gas condensate resistance of the foam-scrubbing agent, the general idea is to preferably select a foam-scrubbing agent main agent (such as an amphoteric surfactant) with better performance, assist a plurality of surfactant auxiliary agents (such as an anionic surfactant, a nonionic surfactant, a fluorocarbon surfactant and the like) with a synergistic effect, and simultaneously, add some foam-assisting agents and auxiliary agents (such as amine oxide, triethanolamine, ethylene glycol and the like) to improve various performances of the whole system (such as CN 102212348A, CN 105154053A, CN 105199702A). From the aspect of field practical application effects, the foam scrubbing agent system prepared by taking the natural surfactant, namely the soapberry extract, as a main agent and assisting with a plurality of surfactant single agents obtains better effects (such as CN 103937479A, CN 102660239A) in some gas fields, and the soapberry extract has certain environmental protection properties and can improve the environmental protection property of the system to a certain extent.
With the rapid development of national economy, especially the problems of extensive and inefficient petrochemical industry, the refinement and greening of resource application gradually become more and more critical problems in the production activities of various industries recently. The biosurfactant is a substance with surface activity secreted in the metabolic process of microorganisms when the microorganisms are cultured under certain conditions, such as glycolipid, lipopeptide or neutral lipid derivative. Compared with chemically synthesized surfactants, the biosurfactants, especially glycolipid biosurfactants, have huge and complex molecular structures, have the advantages of efficiently reducing surface/interface tension, stabilizing emulsion, increasing foam, having good biocompatibility, being nontoxic, biodegradable, having no pollution, specificity, good selectivity and the like, and certain glycolipid biosurfactants also have good antibacterial and bacteriostatic properties, good chemical stability, strong alkali resistance and strong acid resistance, which are difficult to achieve by common chemically synthesized surfactants.
The Chinese patent with the publication number of CN 103555309B discloses a biosurfactant-based water-lock injury treating agent, which comprises the following components in percentage by mass: 30-70% of microbial fermentation liquor, 1-5% of nonionic surfactant, 0.5-1% of fluorocarbon surfactant, 5-8% of cationic surfactant and the balance of distilled water, wherein the microbial fermentation liquor is fermentation liquor of pseudomonas aeruginosa; the water-lock injury treating agent disclosed in the patent can effectively prevent and relieve water-lock injury, but the microbial fermentation liquid and the surfactant are large in dosage, high in cost and easy to affect the environment to a certain extent.
The invention patent with the publication number of CN 103614131B discloses a biochemical salt-resistant high-temperature-resistant oil displacement viscosity reducer and a preparation method thereof, and the oil displacement viscosity reducer disclosed by the patent comprises the following components in percentage by mass: 30-85% of microbial fermentation liquor, 0.5-5% of anionic surfactant, 0.5-10% of nonionic surfactant and the balance of distilled water; the microorganism fermentation broth is a fermentation broth of pseudomonas aeruginosa or bacillus subtilis; the oil displacement viscosity reducer disclosed by the patent has higher high temperature resistance and mineralization resistance, but the dosage of the surfactant and the microbial fermentation liquor is too large, the cost is high, and the environment is damaged.
Disclosure of Invention
The invention aims to provide a salt-resistant high-temperature-resistant foam scrubbing agent which has good methanol resistance and condensate oil resistance.
The second purpose of the invention is to provide a preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.2-0.5% of an anionic-nonionic surfactant composite system, 0.02-0.08% of microbial fermentation liquor and the balance of water; the microbial fermentation liquid is microbial strain fermentation liquid for producing glycolipid biosurfactant or lipopeptide biosurfactant.
The salt-resistant high-temperature-resistant foam scrubbing agent disclosed by the invention takes an anionic-nonionic surfactant complex system as a foam scrubbing agent main agent, and takes microbial fermentation liquor capable of efficiently producing a biosurfactant as a foam promoter, so as to prepare an environment-friendly biochemical composite temperature-resistant salt-resistant foam scrubbing agent; the salt-resistant high-temperature resistant foam scrubbing agent has extremely low contents of a microorganism fermentation liquor and an anionic-nonionic surfactant composite system, but improves the foaming performance and the foam stability of the foam scrubbing agent on one hand, improves the environmental protection property of the foam scrubbing agent on the other hand, can obviously improve the drainage and gas production efficiency of a water-producing gas well, is beneficial to the high and stable yield of a high-liquid-containing gas well, can meet the working conditions of high temperature, high salt, high condensate oil and high methanol content, and has strong adaptability.
The anionic-nonionic surfactant composite system is formed by compounding an anionic surfactant and a nonionic surfactant according to the mass ratio of 30-70: 30-70. According to the invention, after the anionic surfactant and the nonionic surfactant are compounded, a mixed micelle can be formed, and a nonionic molecule is inserted between ion molecules, so that the electric repulsion between head groups of the original ion molecules is weakened, the charge density of micelles is reduced, the cmc (critical micelle concentration) is reduced, when the solution reaches the critical micelle concentration, the surface tension of the solution is reduced to the lowest value, the surface activity is higher, and the expected purpose can be achieved at lower concentration by compounding the anionic surfactant and the nonionic surfactant; in addition, under the electrostatic interaction, the surfactants are staggered in the micelle to form a stable mosaic structure, and macroscopically show the salt resistance.
The anionic surfactant is any one or more of alkyl benzene sulfonate, alpha-olefin sulfonate and higher fatty amide sulfonate.
The above-mentioned alpha-olefin sulfonate is preferably sodium alpha-olefin sulfonate.
The alkylbenzene sulfonate is preferably sodium dodecylbenzenesulfonate.
The above-mentioned higher fatty acid amide sulfonate is preferably sodium N, N-oleoyl methyltaurate.
The nonionic surfactant is one or more of fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene ether, polyoxyethylene amide and sorbitol fatty acid ester.
The sorbitol fatty acid ester is preferably Tween-40.
The alkylphenol polyoxyethylene is preferably nonylphenol polyoxyethylene TX-40.
The polyoxyethylene amide is preferably polyoxyethylene amide 6503.
The microbial fermentation liquid is prepared by fermenting microbial strains which produce glycolipid biosurfactant or lipopeptide biosurfactant.
The microbial strain for producing the glycolipid biosurfactant is pseudomonas aeruginosa. The preservation number of the pseudomonas aeruginosa is CGMCC No. 1.2464.
The microbial strain for producing the lipopeptide biosurfactant is bacillus amyloliquefaciens. The preservation number of the bacillus amyloliquefaciens is CGMCC No. 11950.
The salt-resistant high-temperature-resistant foam scrubbing agent provided by the invention is used as a surfactant system, and can play a role in changing the wettability of a stratum when entering the stratum from a shaft so as to remove/prevent water lock damage, thereby improving the exploitation efficiency of a gas well.
For oil-gas-containing wells, when the foam discharging agent provided by the invention enters a stratum from a shaft, microorganism fermentation liquor is combined with adjacent oil drops through interstitial water and then transferred, microorganisms are adsorbed on the boundaries of the oil drops to convert hydrocarbon molecules into organic solvents, surfactants, acids, alcohols, gases and the like, and the organic solvents are dissolved in petroleum to reduce the viscosity of the oil drops; the surfactant can reduce the interfacial tension between oil and water and between oil and rock, improve the oil displacement efficiency, change the wettability and improve the oil phase flow capacity; the acid can reduce the carbonate content in the oil and pore throats, erode quartz and carbonate surfaces to improve effective permeability; the gas is dissolved in the oil, the viscosity is reduced, and the oil drops are caused to expand to play a physical oil displacement role, so that the exploitation efficiency of the oil-gas-containing well is improved.
The foam scrubbing agent contains an anion-nonionic surfactant composite system, generally speaking, the nonionic surfactant is influenced by the structural characteristics of the nonionic surfactant, and the temperature resistance is limited; the anionic surfactant has poor salt tolerance; the anionic surfactant and the nonionic surfactant are compounded, so that on one hand, the system can obtain better temperature resistance and salt tolerance comprehensive performance, and also can obtain lower cmc and other application performance; the foam discharging agent component also contains microbial fermentation liquor for producing glycolipid or lipopeptide biosurfactant, and the produced surfactant generally takes a rhamnose structure, a polypeptide structure and carboxyl as hydrophilic groups and has the structural characteristics of natural anionic and nonionic surfactants. Therefore, the biosurfactant generated by the microbial fermentation liquor can enhance the synergistic interaction and compatibility of the anion and the nonionic surfactant, and further improve the overall foaming, foam stabilizing and other performances of the system.
The foam scrubbing agent provided by the invention takes fermentation liquor of a strain which can be metabolized to generate a biosurfactant which has strong high temperature resistance and salt resistance and can foam efficiently as a foam promoter, and is compounded with a negative-non-surfactant by an in-situ compounding method to prepare the environment-friendly biochemical composite salt-resistant high-temperature foam scrubbing agent which has good compatibility with formation liquid, is suitable for a gas well drainage and gas production process with the problems of single high temperature, high mineralization degree, high condensate oil content, high methanol content and the like, and is also suitable for a gas well drainage and gas production process with the complex problems of high temperature, high mineralization degree, condensate oil content, high methanol content and the like.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating microbial strains producing glycolipid biosurfactant or lipopeptide biosurfactant on a culture medium, and culturing at 30-42 ℃ for 1-3 days to form colonies;
2) inoculating the bacterial colony obtained in the step 1) into a seed culture medium, and performing shake culture at 35-42 ℃ for 1-4 days to obtain a seed solution;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 30-42 ℃ for 2-5 days, and centrifuging to obtain a microorganism fermentation liquid, wherein the volume ratio of the seed liquid to the fermentation culture medium is 1: 20-50;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the microbial fermentation liquor and water to obtain the microbial fertilizer.
The culture medium in the step 1) is a solid culture medium, wherein the mass ratio of agar powder, peptone, yeast powder and NaCl in the solid culture medium is 15-20: 8-12: 3-5: 8 to 12. The pH value of the solid culture medium is 7-8.
Preferably, the concentration of the agar powder in the solid culture medium is 15-20 g/L, the concentration of the peptone is 8-12 g/L, and the concentration of the yeast powder is 3-5 g/L, NaCl and is 8-12 g/L. The pH value of the solid culture medium is 7-8.
Culturing at 30-42 ℃ for 1-3 days in the step 1), preferably culturing at 30-37 ℃ for 1-2 days.
Inoculating the bacterial colony obtained in the step 1) into a seed culture medium in the step 2), wherein the bacterial colony is a single selected bacterial colony.
The concentration of glucose in the seed culture medium in the step 2) is 8-15 g/L, and NH4The concentration of Cl is 1-3 g/L, K2HPO4·12H2The concentration of O is 2-4 g/L, KH2PO4The concentration of (b) is 0.1-1 g/L, MgSO4·7H2The concentration of O is 0.1-0.5 g/L, FeSO4·7H2The concentration of O is 0.1-0.3 g/L. The pH value of the seed culture medium in the step 2) is 7-8.
In the step 2), the culture is performed for 1-4 days at 35-42 ℃, preferably for 1-2 days at 30-37 ℃ and at the speed of 150-200 rpm.
The concentration of glucose in the fermentation medium in the step 3) is 10-12 g/L, the concentration of L-sodium glutamate is 2-3 g/L, the concentration of yeast powder is 0.5-1 g/L, and K is2HPO4The concentration of (A) is 3-4 g/L, KH2PO4The concentration of (A) is 1.5-2 g/L, FeSO4The concentration of (A) is 0.005-0.01 g/L, MnSO4The concentration of (b) is 0.005-0.01 g/L, MgSO4The concentration of the catalyst is 0.01-0.02 g/L, CaCl2The concentration of (b) is 0.01-0.02 g/L. The pH value of the fermentation medium in the step 3) is 7-8.
And 3) carrying out shake culture at 30-42 ℃ for 2-5 days, preferably at 30-37 ℃ and at the speed of 100-180 rpm for 2-5 days.
The anionic-nonionic surfactant composite system is formed by compounding an anionic surfactant and a nonionic surfactant according to the mass ratio of 30-70: 30-70.
The salt-resistant and high-temperature-resistant foam scrubbing agent has the characteristics of environmental protection, adopts an anionic-nonionic surfactant complex system as a main agent, reduces the critical micelle concentration, reduces the surface tension of a solution, and promotes the expected purpose at a lower concentration, and is compounded with microbial fermentation liquor to obtain the salt-resistant and high-temperature-resistant foam scrubbing agent with salt resistance, high-temperature-resistant foam scrubbing, foam stabilization and liquid carrying capacity, good condensate oil resistance and methanol resistance, and contribution to environmental protection and continuous development of oil and gas fields.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the steps of firstly preparing the microbial fermentation liquor, mixing the microbial fermentation liquor and the anion-nonionic surfactant composite system by using the microbial fermentation liquor in-situ compounding process, avoiding the processes of concentration, extraction and redissolution of the fermentation liquor which are necessary when the biosurfactant is generally applied, and being simple in preparation method, improving the production efficiency and reducing the operation cost.
Drawings
FIG. 1 is an infrared test chart of glycolipid biosurfactant in the Pseudomonas aeruginosa broth of example 1;
FIG. 2 is an infrared test chart of the lipopeptide biosurfactant in the Bacillus amyloliquefaciens fermentation broth of example 5.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings.
Example 1
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.2 percent of anion-nonionic surfactant composite system, 0.02 percent of pseudomonas aeruginosa fermentation liquor for producing glycolipid biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant sodium dodecyl benzene sulfonate and nonionic surfactant fatty alcohol-polyoxyethylene ether AEO 9 according to the weight ratio of 30: 70 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 on LB solid culture medium, and culturing at 35 ℃ for 3 days to form a colony; the LB solid culture medium is: 15g/L of agar powder, 12g/L of peptone, 3g/L of yeast powder, 12g/L of NaCl, 1L of water supplement and 7 of pH;
2) selecting the single colony obtained in the step 1) to inoculate in a seed culture medium, and carrying out shake culture at 35 ℃ for 4 days to obtain a seed solution, wherein the seed culture medium is as follows: glucose 8g/L, NH4Cl 3g/L，K2HPO4·12H2O 2g/L，KH2PO41g/L，MgSO4·7H2O 0.1g/L，FeSO4·7H2O0.3 g/L, adding water to 1L, and the pH value is 7; the rate of the above-mentioned oscillation is 100 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 35 ℃ for 5 days, and centrifuging to obtain a microorganism fermentation liquid, wherein the fermentation culture is carried outThe nutrient base is: 10g/L glucose, 3g/L sodium L-glutamate, 0.5g/L yeast powder, K2HPO44g/L，KH2PO41.5g/L，FeSO40.01g/L，MnSO40.005g/L，MgSO40.02g/L，CaCl20.01g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 100 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 50; the infrared test chart of the glycolipid biosurfactant in the pseudomonas aeruginosa fermentation liquor is shown in figure 1;
4) and uniformly mixing the anion-nonionic surfactant composite system, the pseudomonas aeruginosa fermentation liquor for producing the glycolipid biosurfactant and water according to the proportion to obtain the microbial oil.
Example 2
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.3 percent of anion-nonionic surfactant composite system, 0.04 percent of pseudomonas aeruginosa fermentation liquor for producing glycolipid biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant alpha-olefin sodium sulfonate and nonionic surfactant alkylphenol polyoxyethylene TX-40 according to the weight ratio of 50: 50 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 on LB solid culture medium, and culturing at 37 ℃ for 2d to form a colony; the LB solid culture medium is: 20g/L of agar powder, 8g/L of peptone, 5g/L of yeast powder, 8g/L of NaCl, 1L of water supplement and 8 of pH;
2) selecting the single colony obtained in the step 1) to be inoculated in a seed culture medium, and carrying out shake culture at 42 ℃ for 2d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 15g/L, NH4Cl 1g/L，K2HPO4·12H2O 3g/L，KH2PO42g/L，MgSO4·7H2O0.5g/L，FeSO4·7H2O0.1 g/L, adding water to 1L, and the pH value is 8; the rate of the above-mentioned oscillation is 150 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium at 30 DEG CCarrying out shake culture for 5d, and centrifuging to obtain pseudomonas aeruginosa fermentation liquor, wherein the fermentation medium is as follows: 12g/L glucose, 2g/L sodium L-glutamate, 1g/L yeast powder, K2HPO43g/L，KH2PO42g/L，FeSO40.005g/L，MnSO40.01g/L，MgSO40.01g/L，CaCl20.02g/L, adding water to 1L, and adjusting the pH value to 7; the rate of the above-mentioned oscillation is 150 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 20;
4) and (3) uniformly mixing the anion-nonionic surfactant composite system and the pseudomonas aeruginosa fermentation liquor obtained in the step 3) with water according to the mixture ratio to obtain the pseudomonas aeruginosa fermentation liquor.
Example 3
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.4 percent of anion-nonionic surfactant composite system, 0.06 percent of pseudomonas aeruginosa fermentation liquor for producing glycolipid biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant N, N-oleoyl methyl sodium taurate and nonionic surfactant polyoxyethylene amide 6503 according to the weight ratio of 70:30 and water are mixed evenly.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 on LB solid culture medium, and culturing at 42 ℃ for 1d to form a colony; the LB solid culture medium is: 18g/L of agar powder, 10g/L of peptone, 4g/L of yeast powder and 10g/L of NaCl, supplementing water to 1L and keeping the pH value at 7;
2) selecting the single colony obtained in the step 1) to inoculate in a seed culture medium, and carrying out shake culture at 38 ℃ for 3d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 10g/L, NH4Cl 2g/L，K2HPO4·12H2O 4g/L，KH2PO40.5g/L，MgSO4·7H2O 0.2g/L，FeSO4·7H2O0.2 g/L, adding water to 1L, and the pH value is 7; the rate of the above-mentioned oscillation is 200 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 35 ℃ for 5 days, and centrifuging to obtain the verdigrisA pseudomonas fermentation broth, wherein the fermentation medium is: 10g/L glucose, 3g/L sodium L-glutamate, 0.5g/L yeast powder, K2HPO44g/L，KH2PO41.5g/L，FeSO40.01g/L，MnSO40.005g/L，MgSO40.02g/L，CaCl20.01g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 100 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 50;
4) and (3) uniformly mixing the anion-nonionic surfactant composite system and the pseudomonas aeruginosa fermentation liquor obtained in the step 3) with water according to the mixture ratio to obtain the pseudomonas aeruginosa fermentation liquor.
Example 4
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.5 percent of anion-nonionic surfactant composite system, 0.08 percent of pseudomonas aeruginosa fermentation liquor for producing glycolipid biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant sodium dodecyl benzene sulfonate and nonionic surfactant polyol fatty acid ester Tween-40 according to the weight ratio of 40: 60 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 on LB solid culture medium, and culturing at 30 ℃ for 3d to form a colony; the LB solid culture medium is: 15g/L of agar powder, 12g/L of peptone, 3g/L of yeast powder, 12g/L of NaCl, 1L of water supplement and 7 of pH;
2) selecting the single colony obtained in the step 1) to be inoculated in a seed culture medium, and carrying out shake culture at 35 ℃ for 4d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 8g/L, NH4Cl 3g/L，K2HPO4·12H2O 2g/L，KH2PO41g/L，MgSO4·7H2O0.1g/L，FeSO4·7H2O0.3 g/L, adding water to 1L, and the pH value is 7; the rate of the above-mentioned oscillation is 100 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 35 ℃ for 5 days, and centrifuging to obtain pseudomonas aeruginosa fermentation liquid, wherein the fermentation is carried outThe culture medium is as follows: 10g/L glucose, 3g/L sodium L-glutamate, 0.5g/L yeast powder, K2HPO44g/L，KH2PO41.5g/L，FeSO40.01g/L，MnSO40.005g/L，MgSO40.02g/L，CaCl20.01g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 100 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 50;
4) and (3) uniformly mixing the anion-nonionic surfactant composite system and the pseudomonas aeruginosa fermentation liquor obtained in the step 3) with water according to the mixture ratio to obtain the pseudomonas aeruginosa fermentation liquor.
Example 5
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.2 percent of anion-nonionic surfactant composite system, 0.02 percent of bacillus amyloliquefaciens fermentation liquor for producing lipopeptide biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared by mixing anionic surfactant alpha-olefin sodium sulfonate and nonionic surfactant fatty alcohol-polyoxyethylene ether AEO 9 according to the weight ratio of 50: 50 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Bacillus amyloliquefaciens (CGMCC No.11950) on LB solid culture medium, and culturing at 35 deg.C for 3d to form colony; the LB solid culture medium is: 15g/L of agar powder, 12g/L of peptone, 3g/L of yeast powder, 12g/L of NaCl, 1L of water supplement and 7 of pH;
2) selecting the single colony obtained in the step 1) to be inoculated in a seed culture medium, and carrying out shake culture at 35 ℃ for 4d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 8g/L, NH4Cl 3g/L，K2HPO4·12H2O 2g/L，KH2PO41g/L，MgSO4·7H2O0.1g/L，FeSO4·7H2O0.3 g/L, adding water to 1L, and the pH value is 7; the rate of the above-mentioned oscillation is 200 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, performing shaking culture at 37 ℃ for 3d, and centrifuging to obtain a bacillus amyloliquefaciens fermentation liquid, wherein the fermentation culture medium is as follows: glucose 10g/L, L-Glu3g/L of sodium, 0.5g/L of yeast powder and K2HPO44g/L，KH2PO41.5g/L，FeSO40.01g/L，MnSO40.005g/L，MgSO40.02g/L，CaCl20.01g/L, adding water to 1L, and adjusting the pH value to 7; the rate of the above-mentioned oscillation is 100 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 50; the infrared test chart of the lipopeptide biosurfactant in the obtained bacillus amyloliquefaciens fermentation liquid is shown in a figure 2;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the bacillus amyloliquefaciens fermentation liquid obtained in the step 3) and water according to the proportion to obtain the bacillus amyloliquefaciens.
Example 6
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.3 percent of anion-nonionic surfactant composite system, 0.04 percent of bacillus amyloliquefaciens fermentation liquor for producing lipopeptide biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant N, N-oleoyl methyl sodium taurate and nonionic surfactant alkylphenol polyoxyethylene TX-40 according to the weight ratio of 60: 40 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Bacillus amyloliquefaciens (CGMCC No.11950) on an LB solid culture medium, and culturing for 1d at 42 ℃, wherein the LB solid culture medium is as follows: 20g/L of agar powder, 8g/L of peptone, 5g/L of yeast powder, 8g/L of NaCl, 1L of water supplement and 8 of pH;
2) selecting a single colony of the LB solid culture medium obtained in the step 1) to be inoculated in a seed culture medium, and carrying out shaking culture at 42 ℃ for 2d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 15g/L, NH4Cl 1g/L，K2HPO4·12H2O 4g/L，KH2PO40.1g/L，MgSO4·7H2O 0.5g/L，FeSO4·7H2O0.1 g/L, adding water to 1L, and the pH value is 8; the rate of the above-mentioned oscillation is 180 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 42 ℃ for 2d, and centrifuging to obtain the precipitateThe bacillus subtilis fermentation liquid comprises a fermentation medium: 12g/L glucose, 2g/L sodium L-glutamate, 1g/L yeast powder, K2HPO43g/L，KH2PO42g/L，FeSO40.005g/L，MnSO40.01g/L，MgSO40.01g/L，CaCl20.02g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 180 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 20;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the bacillus amyloliquefaciens fermentation liquid obtained in the step 3) and water according to the proportion to obtain the bacillus amyloliquefaciens.
Example 7
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.4 percent of anion-nonionic surfactant composite system, 0.06 percent of bacillus amyloliquefaciens fermentation liquor for producing lipopeptide biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared from anionic surfactant sodium dodecyl benzene sulfonate and nonionic surfactant polyoxyethylene amide 6503 according to the weight ratio of 50: 50 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Bacillus amyloliquefaciens (CGMCC No.11950) on LB solid culture medium, and culturing at 42 deg.C for 1d to form colony; the LB solid culture medium is: 20g/L of agar powder, 8g/L of peptone, 5g/L of yeast powder, 8g/L of NaCl, 1L of water supplement and 8 of pH;
2) selecting the single colony obtained in the step 1) to be inoculated in a seed culture medium, and carrying out shake culture at 42 ℃ for 2d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 15g/L, NH4Cl 1g/L，K2HPO4·12H2O 4g/L，KH2PO40.1g/L，MgSO4·7H2O0.5g/L，FeSO4·7H2O0.1 g/L, adding water to 1L, and the pH value is 8; the rate of the above-mentioned oscillation is 180 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 40 ℃ for 2d, centrifuging to obtain a bacillus amyloliquefaciens fermentation liquid, and carrying out fermentationThe culture medium is as follows: 11g/L glucose, 2g/L sodium L-glutamate, 1g/L yeast powder, K2HPO43g/L，KH2PO42g/L，FeSO40.005g/L，MnSO40.01g/L，MgSO40.01g/L，CaCl20.02g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 180 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 30;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the bacillus amyloliquefaciens fermentation liquid obtained in the step 3) and water according to the proportion to obtain the bacillus amyloliquefaciens.
Example 8
The salt-resistant high-temperature-resistant foam scrubbing agent comprises the following components in percentage by mass: 0.5 percent of anion-nonionic surfactant composite system, 0.08 percent of bacillus amyloliquefaciens fermentation liquor for producing lipopeptide biosurfactant and the balance of water; wherein the anionic-nonionic surfactant composite system is prepared by mixing anionic surfactant alpha-olefin sodium sulfonate and nonionic surfactant polyol fatty acid ester Tween-40 according to the weight ratio of 50: 50 by mass ratio.
The preparation method of the salt-resistant high-temperature-resistant foam scrubbing agent comprises the following steps:
1) inoculating Bacillus amyloliquefaciens (CGMCC No.11950) on an LB solid culture medium, and culturing for 1d at 42 ℃, wherein the LB solid culture medium is as follows: 20g/L of agar powder, 8g/L of peptone, 5g/L of yeast powder, 8g/L of NaCl, 1L of water supplement and 8 of pH;
2) inoculating the single colony obtained in the step 1) into a seed culture medium, and performing shaking culture at 42 ℃ for 1d to obtain a seed solution, wherein the seed culture medium is as follows: glucose 15g/L, NH4Cl 1g/L，K2HPO4·12H2O 4g/L，KH2PO40.1g/L，MgSO4·7H2O0.5g/L，FeSO4·7H2O0.1 g/L, adding water to 1L, and the pH value is 8; the rate of the above-mentioned oscillation is 180 rpm;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 42 ℃ for 2d, and centrifuging to obtain a bacillus amyloliquefaciens fermentation liquid, wherein the fermentation culture medium is as follows: glucose 12g/L, sodium L-glutamate 2g/L, fermenting1g/L of mother powder, K2HPO43g/L，KH2PO42g/L，FeSO40.005g/L，MnSO40.01g/L，MgSO40.01g/L，CaCl20.02g/L, adding water to 1L, and adjusting the pH value to 8; the rate of the above-mentioned oscillation is 180 rpm; the volume ratio of the seed liquid to the fermentation medium is 1: 20;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the bacillus amyloliquefaciens fermentation liquid obtained in the step 3) and water according to the proportion to obtain the bacillus amyloliquefaciens.
Experimental example 1
The salt-resistant high-temperature resistant foam displacement agents in examples 1 to 8 were subjected to foaming and liquid carrying capacity tests:
the prepared foam scrubbing agent was tested for foaming performance, foam stability and foam carrying capacity at different concentrations and use temperatures using a Roche foam tester according to the test methods in the trade Standard SY/T6465-2000 foamer evaluation method for foam drainage and gas recovery, and the results are shown in Table 1-2.
TABLE 1 foaming, liquid-carrying capacity of the foam-drainage agents prepared in examples 1-8 (70 ℃; degree of mineralization 10X 10)4mg/L)
Figure BDA0001568384250000121
TABLE 2 foaming, liquid-carrying capacity of the foam-drainage agents prepared in examples 1-8 (100 ℃; degree of mineralization 10X 10)4mg/L)
Figure BDA0001568384250000122
Figure BDA0001568384250000131
Experimental example 2
The foam discharging agent in the examples 1 to 8 is subjected to anti-condensate oil and anti-methanol performance tests:
methanol compatibility test: the concentration of each foam scrubbing agent is 1.0 percent; and (3) testing temperature: 70 ℃; degree of mineralization 10X 104mg/L, test time 12h, results are shown in Table 3.
And (3) testing the compatibility of the anti-condensation oil: the concentration of each foam scrubbing agent is 1.0 percent; temperature: 70 ℃; degree of mineralization 10X 104mg/L, test time 12h, and test results are shown in Table 4.
Compatibility test of methanol and condensate: the concentration of each foam scrubbing agent is 1.0 percent; temperature: 70 ℃; degree of mineralization 10X 104mg/L, test time 12h, and test results are shown in Table 5.
The methanol resistance of the foam scrubbing agent is as follows: 70 ℃; degree of mineralization 10X 104mg/L, the results are shown in Table 6.
The anti-condensation performance of the foam scrubbing agent is 70 ℃; degree of mineralization 10X 104mg/L, the results are shown in Table 7.
Table 3 foam remover compatibilities of foam removers in examples 1-8 at different methanol contents
Figure BDA0001568384250000132
Figure BDA0001568384250000141
Table 4 foam-drainage agent compatibility of foam-drainage agents of examples 1-8 at different condensate contents
Figure BDA0001568384250000142
TABLE 5 foam-remover compatibilities of foam-removers from examples 1-8 at different methanol, condensate contents
Figure BDA0001568384250000143
As shown in the compatibility experiments of tables 3-5, the foam scrubbing agent has good compatibility with methanol and mineralization water, and no precipitation phenomenon occurs.
TABLE 6 methanol resistance of the foam scrubbing agents of examples 1-8
Figure BDA0001568384250000151
TABLE 7 anti-gas condensate performance of the foam-drainage agents of examples 1-8
Figure BDA0001568384250000152
According to the results of the methanol resistance and gas condensate coagulation resistance experiments, the salt-resistant and high-temperature resistant foam scrubbing agent can meet the requirements of gas fields with the methanol content of less than or equal to 40 percent and the gas condensate content of less than or equal to 20 percent.

Claims (8)

1. The salt-resistant high-temperature-resistant foam scrubbing agent is characterized by comprising the following components in percentage by mass: 0.2-0.5% of an anionic-nonionic surfactant composite system, 0.02-0.08% of microbial fermentation liquor and the balance of water; the microbial fermentation broth is microbial strain fermentation broth for producing glycolipid biosurfactant or lipopeptide biosurfactant; the microbial strain for producing the glycolipid biosurfactant is pseudomonas aeruginosa, and the preservation number of the pseudomonas aeruginosa is CGMCC No. 1.2464; the microbial strain for producing the lipopeptide biosurfactant is bacillus amyloliquefaciens, and the preservation number of the bacillus amyloliquefaciens is CGMCC No. 11950.
2. The salt-resistant high-temperature-resistant foam scrubbing agent as claimed in claim 1, wherein the anionic-nonionic surfactant complex system is prepared by compounding an anionic surfactant and a nonionic surfactant according to a mass ratio of 30-70: 30-70.
3. The salt-resistant high-temperature resistant foam scrubbing agent as claimed in claim 2, wherein said anionic surfactant is any one or more of alkyl benzene sulfonate, alpha-olefin sulfonate and higher fatty amide sulfonate.
4. The salt-resistant high-temperature-resistant foam-scrubbing agent as claimed in claim 2, wherein said nonionic surfactant is any one or more of fatty alcohol-polyoxyethylene ether, alkylphenol ethoxylate, polyoxyethylene amide, and sorbitol fatty acid ester.
5. The preparation method of the salt-resistant high-temperature resistant foam-elimination agent as claimed in claim 1, which comprises the following steps:
1) inoculating microbial strains producing glycolipid biosurfactant or lipopeptide biosurfactant on a culture medium, and culturing at 30-42 ℃ for 1-3 days to form colonies;
2) inoculating the bacterial colony obtained in the step 1) into a seed culture medium, and performing shake culture at 35-42 ℃ for 1-4 days to obtain a seed solution;
3) inoculating the seed liquid obtained in the step 2) into a fermentation culture medium, carrying out shaking culture at 30-42 ℃ for 2-5 days, and centrifuging to obtain a microorganism fermentation liquid, wherein the volume ratio of the seed liquid to the fermentation culture medium is 1: 20-50;
4) and (3) uniformly mixing the anionic-nonionic surfactant composite system, the microbial fermentation liquor and water to obtain the microbial fertilizer.
6. The preparation method of the salt-resistant high-temperature-resistant foam-elimination agent according to claim 5, wherein the culture medium in the step 1) is a solid culture medium, and the mass ratio of agar powder, peptone, yeast powder and NaCl in the solid culture medium is 15-20: 8-12: 3-5: 8 to 12.
7. The method for preparing the salt-resistant and high-temperature-resistant foam-elimination agent as claimed in claim 5, wherein the concentration of glucose in the seed culture medium in the step 2) is 8-15 g/L, NH4The concentration of Cl is 1-3 g/L, K2HPO4·12H2The concentration of O is 2-4 g/L, KH2PO4The concentration of (b) is 0.1-1 g/L, MgSO4·7H2The concentration of O is 0.1-0.5 g/L, FeSO4·7H2The concentration of O is 0.1-0.3 g/L.
8. The method for preparing the salt-resistant high-temperature resistant foam discharging agent according to claim 5, wherein the concentration of glucose in the fermentation medium in the step 3) is 10-12 g/L, the concentration of L-sodium glutamate is 2-3 g/L, the concentration of yeast powder is 0.5-1 g/L, and K is2HPO4The concentration of (A) is 3-4 g/L, KH2PO4The concentration of (A) is 1.5-2 g/L, FeSO4The concentration of (A) is 0.005-0.01 g/L, MnSO4The concentration of (b) is 0.005-0.01 g/L, MgSO4The concentration of the catalyst is 0.01-0.02 g/L, CaCl2The concentration of (b) is 0.01-0.02 g/L.
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