CN107312515B - A kind of multi-element biologic compound oil displacement agent system and its injection technology - Google Patents
A kind of multi-element biologic compound oil displacement agent system and its injection technology Download PDFInfo
- Publication number
- CN107312515B CN107312515B CN201710405104.4A CN201710405104A CN107312515B CN 107312515 B CN107312515 B CN 107312515B CN 201710405104 A CN201710405104 A CN 201710405104A CN 107312515 B CN107312515 B CN 107312515B
- Authority
- CN
- China
- Prior art keywords
- culture
- liquid
- fermentation
- bacterium
- oil displacement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2208/00—Aspects relating to compositions of drilling or well treatment fluids
- C09K2208/12—Swell inhibition, i.e. using additives to drilling or well treatment fluids for inhibiting clay or shale swelling or disintegrating
Abstract
Of the invention a kind of multi-element biologic compound oil displacement agent system and its injection technology, it is related to oil reservoir oil displacement agent and process for producing same and application method, its oil displacement system is made of petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent, its injection technology operating procedure are as follows: by block well injection station, using injection well and extraction well as defined area, biological compound oil displacement agent 0.2pv is injected by with injection allocation flow velocity and with fluence requirement, then note petroleum hydrocarbon degradation microbial inoculum 0.1pv is taken over, take over the biological compound oil displacement agent 0.2pv of injection, continue to inject petroleum hydrocarbon degradation microbial inoculum 0.1pv, finally inject biological compound oil displacement agent 0.2pv, finally use water drive instead i.e..The present invention after water drive hypotonic heavy crude reservoir, it is at low cost, pollution-free, do not injure oil reservoir；Atmospheric pressure is produced using this system and injection technology, after 76 hours up to 1.0MP or more, system is diluted to concentration 5~10%, and interfacial tension reaches 5.2 × 10‑3~8.2 × 10‑3MN/m, to viscosity in the viscous crude of 10000 mpas, viscosity break ratio reaches 85.7%, and physical analogy improves recovery ratio and averagely reaches 18.0% or more.
Description
Technical field
The present invention relates to a kind of production method of oil reservoir oil displacement agent and application method more particularly to a kind of multi-element biologic are compound
Oil displacement agent system and its injection technology.
Background technique
Petroleum Industry is one of pillar industry of Chinese national economy, has great shadow to the development of Chinese national economy
It rings.Oilfield exploitation experienced primary oil recovery, secondary oil recovery and tertiary oil recovery, tertiary oil recovery include heating power, chemistry, the miscible displacement of reservoir,
Four class technology of microbe oil production, wherein Microbial Enhanced Oil Recovery is at low cost, does not pollute stratum and environment, is one very potential
Raising harvest efficiency technology.Just currently, chemical ternary composite driving technology is both at home and abroad at one of tertiary phase exploitation
Recovery efficiency technique is efficiently improved, but there are high concentration alkali corrosion and scaling, demulsification difficulty, dosage of surfactant are big, at high cost, poly-
The problems such as closing object difficult to degrade and its waste water treatmentntrol difficult.Life is carried out in irrational extraction of resources for chemical ternary composite driving to oil reservoir
Polymer, alkali and chemical table in chemical ternary composite displacement system are replaced in the research of object multiple elements design oil displacement system with biological product
Face activating agent forms degradable, pollution-free and efficient biological oil displacement system, forms efficient green in the following oil field development and adopt
Oil tech is of great significance.
Summary of the invention
The present invention provides a kind of multi-element biologic compound oil displacement agent system and its injection for above-mentioned the deficiencies in the prior art
Technique.
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes, the petroleum hydrocarbon degradation microbial inoculum is mixed by 1~5 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15~20 portions of nutrient solutions
It closes and is uniformly made；
The biological compound oil displacement agent is made of by weight following ingredients: biopolymer bacterium fermenation raw liquid 5~
10 parts, biological 10~20 parts, 1~5 part of glycine betaine of surfactant of lipopeptid, control pH8~9 by 60~80 parts of water；
The nutrient solution is 0.5~5 part of mixed liquor, the phosphoric acid hydrogen two by corn syrup and sucrose by 3:1 quality proportioning
0.1~0.5 part of ammonium, 0.1~0.5 part of sodium nitrate, 0.02~0.05 part of humic acid sylvite, 60~80 parts of water；
It is above mass fraction.
As a further improvement of the present invention, the petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, from
The heart removes confluent monolayer cells, washs cell, is resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25
DEG C overnight incubation, dilutes coated plate, and selection can utilize hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
For single carbon source for growth, be target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18~24 hours, activation bacterium solution is made；
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, potassium dihydrogen phosphate
1.5~2 parts, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control 60
~80%, it is above mass fraction；
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24~30 hours, obtains seed bacterium solution；
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control cultivates 24~36 7.0~8.0,25 ± 2 DEG C of temperature
After hour, primary fermentation liquid is made；
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control cultivate 30 7.0~8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
After~48 hours, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, di(2-ethylhexyl)phosphate
1.5~2 parts of hydrogen potassium, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control
60~80%；
It is above mass fraction, mass percent and mass ratio.
As a further improvement of the present invention, the biopolymer bacterium fermenation raw liquid is obtained by the following method
:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min,
Confluent monolayer cells under centrifuging and taking wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml,
25 DEG C of overnight incubations dilute coated plate, and selection can be that carbon source generates Huang using carbohydrate at a temperature of 20~50 DEG C after screening domestication
The strain of virgin rubber is target strain；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, activation bacterium solution is made；
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, obtains seed bacterium solution；
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.0~8.0, temperature
After 25 ± 2 DEG C, culture 8~13 hours, one grade fermemtation bacterium solution is made；
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control 0.5~0.6vvm of ventilation quantity, 100 revs/min of speed of agitator, pH value control 7.0~8.0,
After 25 ± 2 DEG C of temperature, culture 8~13 hours, second order fermentation bacterium solution is obtained；
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, 0.7~0.8vvm of ventilation quantity, process pH value control are stirred 7.0~8.5,25 ± 2 DEG C of temperature
80 revs/min of speed, culture 7~10 hours after, will fermentation liquid import same volume fermentor in press same process parameter, respectively after
Biopolymer bacterium fermenation raw liquid is made after fermentation 15~20 hours in continuous fermenting and producing；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
10~20 parts, 5~7 parts of yeast extract, 0.2~0.3 part of peptone, 1.5~2 parts of potassium dihydrogen phosphate, epsom salt 0.3~0.5
At 25 ± 2 DEG C of 20~50%, cultivation temperature, pH value 7~8, mixing speed is controlled for part, the control of 0.1~0.2 part of calcium chloride, liquid amount
It is above mass fraction in 80~150rpm；
It is above mass fraction, mass percent and mass ratio.
A kind of injection technology of multi-element biologic compound oil displacement agent system, specific steps are as follows: being injected by block well
It stands, using injection well and extraction well as defined area, injects biological compound oil displacement agent 0.2pv by injection allocation flow velocity and with fluence requirement,
Then note petroleum hydrocarbon degradation microbial inoculum 0.1pv is taken over, the biological compound oil displacement agent 0.2pv of injection is taken over, continues to inject petroleum hydrocarbon degradation
Microbial inoculum 0.1pv finally injects biological compound oil displacement agent 0.2pv, and finally using water drive instead is to complete a full set of injection technology.
Wherein, the preparation method of the biological lipopeptid surfactant in biological compound oil displacement agent be public technology, by
A kind of patent of invention " industrialized process for preparing of the lipopeptide biosurfactant " institute of Patent No. 201110343413.6 is public
It opens.
A kind of multi-element biologic compound oil displacement agent system of the invention, has the advantage that
1) its pH is neutral or alkalescent, after water drive hypotonic heavy crude reservoir, have it is at low cost, pollution-free,
Not the characteristics of not injuring oil reservoir, sustainable oil recovery；
2) it is with endogenous and inoculating microbe oil recovery technique performance, while having both chemical ternary composite driving mechanism,
While driving crude oil, using gas effect, washing oil effect and swept volume effect is produced, promote the extraction of crude oil；
3) petroleum hydrocarbon degradation bacterium is tamed by screening, generates biological polyoses polymer, yield is up to 2.5~3.5%；
4) biological compound oil displacement agent not only can be used as the nutriment of petroleum hydrocarbon degradation bacterium, but also has and increase swept volume,
Liquid mobility is reduced to be compared to use；
5) humic acid sylvite had both been used as microbial nutrition agent, and the effect for promoting viscosity of crude to reduce, which again has system, to be prevented
Clay swell effect；
6) this system and injection technology are utilized, atmospheric pressure is produced after 76 hours up to 1.0MP or more, system is diluted to concentration 5
~10%, interfacial tension reaches 5.2 × 10-3~8.2 × 10-3MN/m, to viscosity in the viscous crude of 10000 mpas, viscosity break ratio reaches
To 85.7%, physical analogy improves recovery ratio and averagely reaches 18.0% or more.
Specific embodiment
Embodiment 1
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 1 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15 portions of nutrient solutions, and mixing is equal
It is even to be made；
The biological compound oil displacement agent is made of by weight following ingredients: 5 parts of biopolymer bacterium fermenation raw liquid,
10 parts of biological lipopeptid surfactant, 1 part of glycine betaine, control pH8, system viscosity 47mpas by 60~80 parts of water；
The nutrient solution is 0.5 part of mixed liquor, the diammonium hydrogen phosphate by corn syrup and sucrose by 3:1 quality proportioning
0.1 part, 0.1 part of sodium nitrate, 0.02 part of humic acid sylvite, 60 parts of water；
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, in LB(Luria-Bertani) be resuspended in culture medium, its cell concentration is 1.0 × 108~2.0 ×
108A/ml, 25 DEG C of overnight incubations, dilute coated plate, selection can under the conditions of 10 DEG C~25 DEG C of temperature, using hexadecane,
Benzene, phenanthrene and hexamethylene are single carbon source for growth, are target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18 hours, activation bacterium solution is made；
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2 parts of ammonium chloride, potassium dihydrogen phosphate 1.5
Part, the control of 0.5 part of epsom salt, 0.1 part of manganese chloride, 0.2 part of calcium chloride, liquid amount are mass fraction 60% above；
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24 hours, obtains seed bacterium solution；
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.0,25 ± 2 DEG C of temperature, culture 24 hours, system
Obtain primary fermentation liquid；
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 30 hours 7.0,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more；
It is above mass fraction, mass percent and mass ratio；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5 part of liquid paraffin, 2 parts of ammonium chloride, potassium dihydrogen phosphate
1.5 parts, 0.5 part of epsom salt, 0.1 part of manganese chloride, 0.2 part of calcium chloride, liquid amount control in 40 DEG C of 80%, cultivation temperature, no
Blowing air, mixing speed control are mass fraction in 50pm above.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 600 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Remove confluent monolayer cells, cell washed, in LB(Luria-Bertani) be resuspended in culture medium, its cell concentration is 1.0 × 108~2.0
×108A/ml, 25 DEG C of overnight incubations dilute coated plate, and selection can be carbon using carbohydrate at a temperature of 20 DEG C after screening domestication
The strain that source generates xanthan gum is target strain；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10 hours, activation bacterium solution is made；
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10 hours, obtains seed bacterium solution；
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.0, temperature 25 ± 2
DEG C, after culture 8 hours, one grade fermemtation bacterium solution is made；
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 100 revs/min of speed of agitator, pH value control is 7.0, temperature 25 ± 2
DEG C, after culture 8 hours, obtain second order fermentation bacterium solution；
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.7vvm, process pH value control 7.0,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 7 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermenting and producing respectively, sent out
After ferment 15 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide accounts in microbial fermentation liquid product
0.8%~1.1%；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
10 parts, 5 parts of yeast extract, 0.2 part of peptone, 1.5 parts of potassium dihydrogen phosphate, 0.3 part of epsom salt, 0.1 part of calcium chloride, liquid amount
At 25 ± 2 DEG C of 20%, cultivation temperature, pH value 7, mixing speed is controlled in 80rpm for control；
It is above mass fraction, mass percent and mass ratio.
Embodiment 2
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 5 parts of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 20 portions of nutrient solutions, and mixing is equal
It is even to be made；
The biological compound oil displacement agent is made of by weight following ingredients: biopolymer bacterium fermenation raw liquid 10
Part, biological 20 parts, 5 parts of glycine betaine of surfactant of lipopeptid, control pH9, system viscosity 78mpas by 60~80 parts of water；
The nutrient solution is 5 parts of mixed liquor, the diammonium hydrogen phosphate 0.5 by corn syrup and sucrose by 3:1 quality proportioning
Part, 0.5 part of sodium nitrate, 0.05 part of humic acid sylvite, 80 parts of water；
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, from
The heart removes confluent monolayer cells, washs cell, is resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25
DEG C overnight incubation, dilutes coated plate, and selection can utilize hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
For single carbon source for growth, be target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24 hours, activation bacterium solution is made；
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 3 parts of ammonium chloride, potassium dihydrogen phosphate 2
Part, the control of 1 part of epsom salt, 0.5 part of manganese chloride, 0.5 part of calcium chloride, liquid amount are mass fraction 80% above；
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 30 hours, obtains seed bacterium solution；
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is cultivated 24~36 hours 8.0,25 ± 2 DEG C of temperature
Afterwards, primary fermentation liquid is made；
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 48 hours 8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 1 part of liquid paraffin, 3 parts of ammonium chloride, 2 parts of potassium dihydrogen phosphate,
1 part of epsom salt, 0.5 part of manganese chloride, 0.5 part of calcium chloride, liquid amount control 45 DEG C of 80%, cultivation temperature, not blowing air,
Mixing speed is controlled in 80rpm；
It is above mass fraction, mass percent and mass ratio.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be at 50 °C the bacterium that carbon source generates xanthan gum using carbohydrate after screening domestication
Kind is target strain；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 115 hours, activation bacterium solution is made；
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 15 hours, obtains seed bacterium solution；
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 8.0, temperature 25 ± 2
DEG C, after culture 13 hours, one grade fermemtation bacterium solution is made；
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.6vvm, and 100 revs/min of speed of agitator, pH value control is 8.0, temperature 25 ± 2
DEG C, after culture 13 hours, obtain second order fermentation bacterium solution；
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.8vvm, process pH value control 8.5,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 10 hour, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermenting and producing respectively,
After fermentation 20 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide is in microbial fermentation liquid product
Account for 0.8%~1.1%；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
20 parts, 7 parts of yeast extract, 0.3 part of peptone, 2 parts of potassium dihydrogen phosphate, 0.5 part of epsom salt, 0.2 part of calcium chloride, liquid amount control
At 25 ± 2 DEG C of 50%, cultivation temperature, pH value 8, mixing speed is controlled in 150rpm system；
It is above mass fraction, mass percent and mass ratio.
Embodiment 3
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 3 parts of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 18 portions of nutrient solutions, and mixing is equal
It is even to be made；
The biological compound oil displacement agent is made of by weight following ingredients: 8 parts of biopolymer bacterium fermenation raw liquid,
15 parts of biological lipopeptid surfactant, 3 parts of glycine betaine, control pH8.5, system viscosity 65mpas by 60~80 parts of water；
The nutrient solution is 3 parts of mixed liquor, the diammonium hydrogen phosphate 0.3 by corn syrup and sucrose by 3:1 quality proportioning
Part, 0.3 part of sodium nitrate, 0.05 part of humic acid sylvite, 70 parts of water；
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 700 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C of trainings
It supports overnight, dilutes coated plate, selection can be single using hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
One carbon source for growth is target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 20 hours, activation bacterium solution is made；
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2.5 parts of ammonium chloride, potassium dihydrogen phosphate
1.8 parts, 0.8 part of epsom salt, 0.3 part of manganese chloride, 0.3 part of calcium chloride, liquid amount control 70%, be above mass parts
Number；
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 20 hours, obtains seed bacterium solution；
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.5,25 ± 2 DEG C of temperature, culture 30 hours, system
Obtain primary fermentation liquid；
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 40 hours 7.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.8 part of liquid paraffin, 2.8 parts of ammonium chloride, potassium dihydrogen phosphate
1.8 parts, 0.8 part of epsom salt, 0.3 part of manganese chloride, 0.4 part of calcium chloride, liquid amount control in 80%, cultivation temperature 40~45
DEG C, blowing air, mixing speed control in 780rpm；
It is above mass fraction, mass percent and mass ratio.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 700 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be that carbon source generates xanthan gum using carbohydrate at a temperature of 20~50 DEG C after screening domestication
Strain be target strain；
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 12 hours, activation bacterium solution is made；
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 12 hours, obtains seed bacterium solution；
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.5, temperature 25 ± 2
DEG C, after culture 10 hours, one grade fermemtation bacterium solution is made；
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.55vvm, and 100 revs/min of speed of agitator, pH value control is 7.8, temperature 25 ± 2
DEG C, after culture 10 hours, obtain second order fermentation bacterium solution；
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.75vvm, process pH value control is 7. 5,25 ± 2 DEG C of temperature, mixing speed 80
Rev/min, after culture 8 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermentation life respectively
It produces, after fermentation 18 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide is in microbial fermentation liquid product
In account for 0.8%~1.1%；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
15 parts, 6 parts of yeast extract, 0.25 part of peptone, 1.8 parts of potassium dihydrogen phosphate, 0.4 part of epsom salt, 0.15 part of calcium chloride, dress liquid
At 25 ± 2 DEG C of 30%, cultivation temperature, pH value 7.5, mixing speed is controlled in 100rpm for amount control；
It is above mass fraction, mass percent and mass ratio.
In the application, the one grade fermemtation tank is the tank body that capacity is 100L or 200L；Second order fermentation tank is that capacity is
1m3Tank body；Three grade fermemtation tank is that capacity is 10m3Tank body.
Using petroleum hydrocarbon degradation microbial inoculum obtained by embodiment 1, embodiment 2 or embodiment 3 and biological compound oil displacement agent,
Specific injection technology operating procedure is as follows: by block well injection station, using injection well and extraction well as defined area, by with beam
It speed and requires to inject biological compound oil displacement agent 0.2pv with fluence, then takes over note petroleum hydrocarbon degradation microbial inoculum 0.1pv, take over injection
Biological compound oil displacement agent 0.2pv, continues to inject petroleum hydrocarbon degradation microbial inoculum 0.1pv, finally injects biological compound oil displacement agent 0.2pv,
Finally using water drive instead is to complete a full set of injection technology.
Oil displacement experiment is carried out to a variety of rock cores using injection technology of the invention, experimental result is as follows:
1, oil displacement experiment is carried out to artificial core:
It chooses artificial core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 1:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 21.4% in artificial core.
2, oil displacement experiment is carried out to Berea core:
It chooses Berea core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 2:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 20.9% on Berea core.
3, oil displacement experiment is carried out to natural core:
It chooses natural core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 3:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 18.1% on natural core.
Claims (3)
1. a kind of multi-element biologic compound oil displacement agent system is made of petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent:
The petroleum hydrocarbon degradation microbial inoculum is by 1~5 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15~20 portions of nutrient solutions, mixing
Uniformly it is made；
The biological compound oil displacement agent is made by weight of following component: 5~10 parts of biopolymer bacterium fermenation raw liquid,
10~20 parts of biological lipopeptid surfactant, 1~5 part of glycine betaine, 60~80 parts of water；
The nutrient solution is 0.5~5 part of mixed liquor, the diammonium hydrogen phosphate 0.1 by corn syrup and sucrose by 3:1 quality proportioning
~0.5 part, 0.1~0.5 part of sodium nitrate, 0.02~0.05 part of humic acid sylvite, 60~80 parts of water；
It is above mass fraction.
2. a kind of multi-element biologic compound oil displacement agent system as described in claim 1, it is characterised in that the petroleum hydrocarbon degradation
Bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation being incubated overnight, fermentation liquid to be mutagenic is made, and then will
Fermentation liquid to be mutagenic carries out 600~750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C of trainings
It supports overnight, dilutes coated plate, selection can be single using hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
One carbon source for growth is target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability；
B, prepared by fermentation liquid:
A, target actication of culture: inclined-plane target strain chooses 2 rings and activates training into the 500ml shaking flask of the fluid nutrient medium containing 300ml
It supports, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18~24 hours, activation bacterium solution is made；
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%, inoculation
Amount 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24~30 hours, obtains seed bacterium solution；
C, primary fermentation culture: seed bacterium solution made from b step being imported in first order seed fermentor, seeding tank liquid amount 80%,
Inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.0~8.0,25 ± 2 DEG C of temperature, culture 24~36 hours, system
Obtain primary fermentation liquid；
D, fermented and cultured: primary fermentation liquid made from step c is imported into three grade fermemtation tank, three grade fermemtation tank is pressed 60% liquid amount, connect
Kind amount 5%, process pH value control is after 7.0~8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed, culture 30~48 hours, system
Obtain petroleum hydrocarbon degradation bacterium fermenation raw liquid；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, potassium dihydrogen phosphate
1.5~2 parts, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control 60
~80%；
It is above mass fraction, mass percent and mass ratio.
3. a kind of multi-element biologic compound oil displacement agent system as described in claim 1, it is characterised in that the biopolymer
Bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, then
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing, is mixed, 25 DEG C of heat preservation 30min, is centrifuged according to 1:1
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be that carbon source generates xanthan gum using carbohydrate at a temperature of 20~50 DEG C after screening domestication
Strain be target strain；
B, prepared by fermentation liquid:
A, target actication of culture: inclined-plane target strain chooses 2 rings and activates training into the 500ml shaking flask of the fluid nutrient medium containing 100ml
It supports, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, activation bacterium solution is made；
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%, inoculation
Amount 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, obtains seed bacterium solution；
C, one grade fermemtation culture: seed bacterium solution made from b step being imported in first order seed fermentor, seeding tank liquid amount 50%,
Inoculum concentration 10% controls ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is trained 7.0~8.0,25 ± 2 DEG C of temperature
After supporting 8~13 hours, one grade fermemtation bacterium solution is made；
D, second order fermentation culture: one grade fermemtation bacterium solution made from step c is imported into secondary seed tank, the canned liquid measure 50% of second level connects
Kind of amount 10%, controls 0.5~0.6vvm of ventilation quantity, 100 revs/min of speed of agitator, pH value control is 7.0~8.0, temperature 25 ± 2
DEG C, after culture 8~13 hours, obtain second order fermentation bacterium solution；
E, fermented and cultured: importing three grade fermemtation tank for second order fermentation bacterium solution made from Step d, and three grade fermemtation tank presses 50% liquid amount,
Inoculum concentration 10%, 0.7~0.8vvm of ventilation quantity, process pH value control 7.0~8.5,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 7~10 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermentation life respectively
It produces, after fermentation 15~20 hours, biopolymer bacterium fermenation raw liquid is made；
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup and sucrose by 3:1 quality proportioning mixed liquor 10~
20 parts, 5~7 parts of yeast extract, 0.2~0.3 part of peptone, 1.5~2 parts of potassium dihydrogen phosphate, 0.3~0.5 part of epsom salt, chlorine
Change 0.1~0.2 part of calcium, liquid amount control at 25 ± 2 DEG C of 20~50%, cultivation temperature, pH value 7~8, mixing speed is controlled 80
~150rpm；
It is above mass fraction, mass percent and mass ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710405104.4A CN107312515B (en) | 2017-06-01 | 2017-06-01 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710405104.4A CN107312515B (en) | 2017-06-01 | 2017-06-01 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107312515A CN107312515A (en) | 2017-11-03 |
| CN107312515B true CN107312515B (en) | 2019-09-27 |
Family
ID=60181615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710405104.4A Active CN107312515B (en) | 2017-06-01 | 2017-06-01 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107312515B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110939419A (en) * | 2018-09-25 | 2020-03-31 | 中国石油化工股份有限公司 | Method for improving recovery ratio by efficiently mutagenizing endogenous microorganisms of oil reservoir |
| CN109897621A (en) * | 2019-03-25 | 2019-06-18 | 大庆华理生物技术有限公司 | A kind of binary biology oil displacement agent and its application |
| CN110452866A (en) * | 2019-08-26 | 2019-11-15 | 北京大学 | A kind of activator that orientation activates core microorganism and its application in microbe oil production |
| CN114426834A (en) * | 2020-10-14 | 2022-05-03 | 中国石油化工股份有限公司 | Biological aerosol system and preparation method and application thereof |
| CN114836186B (en) * | 2022-04-21 | 2023-12-12 | 大庆华理生物技术股份有限公司 | Biological thickened oil viscosity reducing agent and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1540137A (en) * | 2003-10-28 | 2004-10-27 | 华东理工大学 | Combined typed method of edge-water encroachment of microbe |
| CN1558085A (en) * | 2004-01-13 | 2004-12-29 | 天津南开戈德集团有限公司 | Microorganism oil production method |
| CN101130754A (en) * | 2007-08-01 | 2008-02-27 | 中国石油化工股份有限公司 | High-density ferment method for petroleum hydrocarbon degradation bacterium |
| CN101130684A (en) * | 2006-08-25 | 2008-02-27 | 上海中油企业集团有限公司 | Complex microorganism preparations for oil production |
| CN101839126A (en) * | 2010-03-25 | 2010-09-22 | 西安石油大学 | Method for increasing production by compounding microorganism and biologically active agent |
| CN101975051A (en) * | 2010-10-19 | 2011-02-16 | 大庆油田有限责任公司 | Chemical flooding alternate injection method |
| CN102213088A (en) * | 2010-04-12 | 2011-10-12 | 北京大学 | Microbial oil recovery method |
| CN102562012A (en) * | 2010-12-27 | 2012-07-11 | 中国石油天然气股份有限公司 | Method for improving recovery ratio of normal heavy oil reservoirs in water-flooding development |
| CN103146677A (en) * | 2013-01-27 | 2013-06-12 | 盐城师范学院 | Improvement method for hydrocarbon degrading bacteria capable of producing surfactant |
| CN103160452A (en) * | 2013-01-27 | 2013-06-19 | 盐城师范学院 | Method capable of selecting and screening surfactant hydrocarbon degradation bacteria on surface of forming material |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2531963C (en) * | 2003-07-14 | 2013-01-08 | The Energy Research Institute | A process for enhanced recovery of crude oil from oil wells using novel microbial consortium |
| US20090082227A1 (en) * | 2007-08-24 | 2009-03-26 | Hnatow Linda L | Application of anaerobic denitrifying bacteria utilizing petroleum components as sole carbon source for oil |
| US7740063B2 (en) * | 2008-08-20 | 2010-06-22 | E.I. Du Pont De Nemours And Company | Method for identification of novel anaerobic denitrifying bacteria utilizing petroleum components as sole carbon source |
| US7708065B2 (en) * | 2008-09-29 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Identification, characterization, and application of Thauera sp. AL9:8 useful in microbially enhanced oil recovery |
-
2017
- 2017-06-01 CN CN201710405104.4A patent/CN107312515B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1540137A (en) * | 2003-10-28 | 2004-10-27 | 华东理工大学 | Combined typed method of edge-water encroachment of microbe |
| CN1558085A (en) * | 2004-01-13 | 2004-12-29 | 天津南开戈德集团有限公司 | Microorganism oil production method |
| CN101130684A (en) * | 2006-08-25 | 2008-02-27 | 上海中油企业集团有限公司 | Complex microorganism preparations for oil production |
| CN101130754A (en) * | 2007-08-01 | 2008-02-27 | 中国石油化工股份有限公司 | High-density ferment method for petroleum hydrocarbon degradation bacterium |
| CN101839126A (en) * | 2010-03-25 | 2010-09-22 | 西安石油大学 | Method for increasing production by compounding microorganism and biologically active agent |
| CN102213088A (en) * | 2010-04-12 | 2011-10-12 | 北京大学 | Microbial oil recovery method |
| CN101975051A (en) * | 2010-10-19 | 2011-02-16 | 大庆油田有限责任公司 | Chemical flooding alternate injection method |
| CN102562012A (en) * | 2010-12-27 | 2012-07-11 | 中国石油天然气股份有限公司 | Method for improving recovery ratio of normal heavy oil reservoirs in water-flooding development |
| CN103146677A (en) * | 2013-01-27 | 2013-06-12 | 盐城师范学院 | Improvement method for hydrocarbon degrading bacteria capable of producing surfactant |
| CN103160452A (en) * | 2013-01-27 | 2013-06-19 | 盐城师范学院 | Method capable of selecting and screening surfactant hydrocarbon degradation bacteria on surface of forming material |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
Non-Patent Citations (1)
| Title |
|---|
| 一株重烃降解菌的分离和生长特性及驱油性能评价;林怀刚等;《化学与生物工程》;20101231;第27卷(第5期);第69-71页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107312515A (en) | 2017-11-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107312515B (en) | A kind of multi-element biologic compound oil displacement agent system and its injection technology | |
| CN107159690A (en) | The preparation method and implementing process of a kind of oil-polluted soils renovation agent system | |
| CN101285047B (en) | D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof | |
| CN102911902B (en) | Anoxybacillussp and application thereof | |
| CN102229920B (en) | Method for improving submerged fermentation level of trichoderma reesei cellulase liquid | |
| CN104373094B (en) | A kind of hyposmosis oil pool microbial oil recovery compound formulation and its application method | |
| CN107558968B (en) | A kind of method that oil well microbial compound throughput recovers the oil | |
| CN102852497B (en) | A kind of compound microorganism oil extraction method for low permeability oilfield | |
| CN108102995A (en) | A kind of D-Psicose 3- epimerases production bacterial strain and its process for fixation | |
| CN104232722A (en) | Method for producing 9-alpha-hydroxyandrostenedione by microbial fermentation | |
| CN102587875B (en) | A kind of use improves the method for crude output containing the composite bacterial solution synergy of phosphorus decomposing and nitrogen-fixing bacteria | |
| CN102492740A (en) | Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans | |
| CN102213087B (en) | Throughout and displacement combined microbial oil recovery method | |
| CN111205842A (en) | Microbial oil production process technology for improving oil recovery ratio | |
| CN105567204B (en) | A method of dolostone reservoirs Central Plains oil recovery is improved using microbial flora | |
| CN103232966A (en) | Middle and high temperature resisting exopoly water insoluble polysaccharide protoplast fusion engineering bacterium and application thereof | |
| CN104498422A (en) | Habituation culture method of psychrophilic methanogens | |
| CN109576191A (en) | A kind of complex micro organism fungicide of heavy oil development and its preparation method and application | |
| CN103114063B (en) | Strain for producing fucosan sulfatase and application thereof | |
| CN102605017A (en) | Method for preparing gellan gum by fermenting | |
| CN112725372B (en) | Multi-enzyme complex vector for improving expression of welan gum and recombinant bacterium for high yield of welan gum | |
| CN103602648B (en) | A kind of zymotechnique improving cellulase production level | |
| CN107794285A (en) | A kind of preparation method and application of displacement of reservoir oil biopolymer | |
| CN106906163A (en) | A kind of Sphingol single-cell WG produces the zymotechnique of Weilan gum | |
| CN104804717B (en) | A kind of foam blocking agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 163000 No. 328, Nansan Road, Honggang District, Daqing City, Heilongjiang Province Patentee after: Daqing Huali Biotechnology Co.,Ltd. Address before: 163000 No.21, Nansan Road, Honggang District, Daqing City, Heilongjiang Province Patentee before: DAQING HUALI BIOTECHNOLOGY Co.,Ltd. |