CN109576191A - A kind of complex micro organism fungicide of heavy oil development and its preparation method and application - Google Patents
A kind of complex micro organism fungicide of heavy oil development and its preparation method and application Download PDFInfo
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- CN109576191A CN109576191A CN201910019197.6A CN201910019197A CN109576191A CN 109576191 A CN109576191 A CN 109576191A CN 201910019197 A CN201910019197 A CN 201910019197A CN 109576191 A CN109576191 A CN 109576191A
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- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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Abstract
The invention discloses complex micro organism fungicides of a kind of heavy oil development and its preparation method and application, are related to Microbial Enhanced Oil Recovery field, are the problem of existing Microbial Enhanced Oil Recovery preparation complexity, single culture are intolerant to environmental impact and propose.Complex micro organism fungicide of the invention after bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus fermentation by being mixed.The beneficial effects of the present invention are: it avoids not tolerating the defect of environmental impact because of single function strain, the functional expression between three kinds of bacterial strains is not suppressed, and preparation method is simple, at low cost.
Description
Technical field
The present invention relates to Microbial Enhanced Oil Recovery fields, and in particular to a kind of complex micro organism fungicide of heavy oil development and its
Preparation method and application.
Background technique
With China's rapid economic development, the demand to petroleum increasingly increases, and oil shortage is also increasingly severe.However it is existing
There are heavy oil reserves to already exceed thin oil reserves, but the Technology of Heavy Oil Recovery lacks very much, is substantially adopted so that thermal recovery is gentle
Based on, energy consumption is high, and investment is high, and validity period short disadvantage be widely applied these technologies can not, how heavy crude producing resource
The problem of being people's strong interest.The principal contradiction problem of thickened oil recovery is viscosity height, and aromatic hydrocarbon content is high.
Microbial Enhanced Oil Recovery (MEOR) is to utilize vital movement and its metabolism of the microorganism in oil reservoir
Product improves a kind of biotechnology of crude output, has and has a wide range of application, implementation cost is low, the spies such as remarkable in economical benefits
Point, increasingly attracts widespread attention.
Mechanism needed for microbial oil displacement is used for heavy oil development generates gas during specifically including that microbial growth and metabolism
Body increases strata pressure, reduces viscosity of crude；Generating biosurfactant and organic solvent reduces oil water interfacial tension, improves
Displacement efficiency；Degrading crude oil heavy component is to reduce viscosity of crude, improve crude oil fluidity etc..
Its metabolic characteristic of different microorganism fungus kinds is different, and the metabolite of generation is different, and mechanism of oil displacement is also different, by
This also forms different microbe oil production technique, and existing microbe oil production microbial inoculum preparation is complicated, using single culture into
When row recovers the oil, single culture does not tolerate environmental impact, reduces oil production rate.
Summary of the invention
Present invention solves the technical problem that being existing Microbial Enhanced Oil Recovery preparation complexity, single culture intolerant to environment
Impact.
The present invention adopts the following technical solutions solves above-mentioned technical problem:
Bacterial strain explanation: bacillus pallidus ATCC 51176 (Bacillus pallidus) used in the present invention, general bacterium
Belong to ATCC11540 (Pantoea) and is purchased from U.S.'s typical strain collection；Thermophilic anaerobic bacillus DSM10170
(Thermoanaerobacterium aotearoense) is purchased from Germany Microbiological Culture Collection Center.
A kind of complex micro organism fungicide of heavy oil development, the complex micro organism fungicide is by bacillus pallidus
(Bacillus pallidus), general Pseudomonas (Pantoea), thermophilic anaerobic bacillus (Thermoanaerobacterium
Aotearoense it is mixed after) fermenting.
Preferably, the preparation method of the complex micro organism fungicide, comprising the following steps:
Bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus are inoculated in fluid nutrient medium cultivate respectively, then
By containing above-mentioned strain culture medium according to strain quantitative proportion be bacillus pallidus: general Pseudomonas: thermophilic anaerobic bacillus=(1-
4): (1-4): the ratio mixing of (1-4).
The present invention also provides a kind of preparation methods of the complex micro organism fungicide of heavy oil development, comprising the following steps:
Bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus are inoculated in fluid nutrient medium cultivate respectively, then
It is proportionally bacillus pallidus by the culture medium containing above-mentioned strain: general Pseudomonas: thermophilic anaerobic bacillus=(1-4): (1-
4): the ratio mixing of (1-4).
Preferably, the fluid nutrient medium is made of the raw material of following weight percent: the corn syrup of 0.5-2.5%,
The KH of the vitamin of 0.5-2.5%, 0.1-0.5%2PO4, 0.01-0.1% NaNO3, 0.1-0.2% yeast powder, 0.01-
0.1% benzene, the xanthan gum of 0.1-0.2%, surplus are water.
Preferably, the pH of the fluid nutrient medium is 7-8.
Preferably, the condition of culture is in 30-40 DEG C of culture 36-50h.
Preferably, the bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus inoculum concentration be respectively Liquid Culture base weight
Measure 1-5%, 1-10%, 1-5% of score.
Preferably, the bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus inoculum concentration be respectively Liquid Culture base weight
Measure 1%, 1%, the 3% of score.
The present invention also provides a kind of application of the complex micro organism fungicide of heavy oil development in oil recovery.
Preferably, complex micro organism fungicide disposably or is in batches injected in oil well.
The beneficial effects of the present invention are:
(1) oil recovery recovery ratio can be improved using complex micro organism fungicide prepared by the present invention；
(2) avoid not tolerating the defect of environmental impact because of single function strain, the functional expression between three kinds of bacterial strains not by
To inhibition；
(3) preparation method of the present invention is simple, at low cost, simplifies the preparation of the complex micro organism fungicide with oil recovery function
Technique effectively raises oil recovery effect.
Specific embodiment
The effect of to make to structure feature of the invention and being reached, has a better understanding and awareness, to preferable
The present invention will be described in detail for embodiment.
Embodiment 1
A kind of preparation method of the complex micro organism fungicide of heavy oil development, comprising the following steps:
(1) fluid nutrient medium is prepared, is wherein made in the culture medium of the raw material of following weight percent: 0.5% jade
Rice syrup, 0.5% vitamin, 0.1% KH2PO4, 0.01% NaNO3, 0.1% yeast powder, 0.01% benzene, 0.1%
Xanthan gum, surplus be water；The pH value for adjusting the culture medium is 7, is sterilized；Wherein benzene both can be used as carbon source and can also make
For solubilizer；Wherein vitamin can be one of vitamin A, vitamin B, vitamin C, vitamin D, vitamin E；
It (2) is respectively fluid nutrient medium weight point according to the inoculum concentration of bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus
1% several ratios carries out inoculated and cultured, incubation time 36h respectively, and cultivation temperature is 30 DEG C；
(3) cultured bacterial strain is mixed according to 1:1:1, obtains complex micro organism fungicide.
Embodiment 2
A kind of preparation method of the complex micro organism fungicide of heavy oil development, comprising the following steps:
(1) fluid nutrient medium is prepared, is wherein made in the culture medium of the raw material of following weight percent: 1.5% jade
Rice syrup, 1.5% vitamin, 0.2% KH2PO4, 0.05% NaNO3, 0.15% yeast powder, 0.05% benzene,
0.15% xanthan gum, surplus are water；The pH value for adjusting the culture medium is 7.5, is sterilized；Wherein benzene both can be used as carbon source
It can also be used as solubilizer；
It (2) is respectively fluid nutrient medium weight point according to the inoculum concentration of bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus
2% several ratios carries out inoculated and cultured, incubation time 45h respectively, and cultivation temperature is 35 DEG C；
(3) cultured bacterial strain is mixed according to 2:1:1, obtains complex micro organism fungicide.
Embodiment 3
A kind of preparation method of the complex micro organism fungicide of heavy oil development, comprising the following steps:
(1) fluid nutrient medium is prepared, is wherein made in the culture medium of the raw material of following weight percent: 2.5% jade
Rice syrup, 2.5% vitamin, 0.5% KH2PO4, 0.1% NaNO3, 0.2% yeast powder, 0.1% benzene, 0.2%
Xanthan gum, surplus are water；The pH value for adjusting the culture medium is 8, is sterilized；Wherein benzene both can be used as carbon source and can also be used as
Solubilizer；
It (2) is respectively fluid nutrient medium weight point according to the inoculum concentration of bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus
5% several ratios carries out inoculated and cultured, incubation time 50h respectively, and cultivation temperature is 40 DEG C；
(3) cultured bacterial strain is mixed according to 1:1:3, obtains complex micro organism fungicide.
Experimental result: complex micro organism fungicide disposably or is in batches injected in low yield heavy oil wells according to test block oil
Aqueous nature, geological reservoir feature determine injection rate, and being suitable for underground viscosity is 50000 centipoises viscous crude below, inject bacterial strain
Inoculum density is 1-5%, if inject in batches, injection number is 1 year 4-12 times；Low yield is had chosen at huge port and Xinjiang Oilfield
Heavy oil wells have carried out microbial single well stimulation test, and using oil pipe addition method, complex micro organism fungicide is added, disposable to inject,
Closing well drives a well after 20 days and resumes production, day oil-producing risen to from 0.2-1.5t/ days 3.4-8.9t/ days, oil increasing effect is obvious.
Table 1 is the experiment knot containing bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus culture medium under different proportion
Fruit:
Viscous crude can reduce its viscosity after emulsifying, from table 1 it follows that when containing bacillus pallidus, general Pseudomonas,
When the volume ratio of thermophilic anaerobic bacillus culture medium is 1:3:1, gas production and emulsifying effectiveness are preferable.
Table 2 is the experimental result comparison of single culture and complex micro organism fungicide:
As can be seen from Table 2, Mixed Microbes be applicable in underground crude oil viscosity be higher than single bacterium treatment effect, while Mixed Microbes have compared with
High-salt tolerance, pH value is applied widely, and activity is preferable within the scope of pH value 4-8, and can make under 20-60 DEG C of temperature range
With, therefore the complex micro organism fungicide prepared is resistant to the impact of environment, avoids because single function strain is intolerant to environmental impact
Defect.
The above is only the preferred embodiment of the present invention, protection scope of the present invention is not limited in above-described embodiment, with
The various process programs of present inventive concept indifference are within the scope of the invention.
Claims (10)
1. a kind of complex micro organism fungicide of heavy oil development, it is characterised in that: the complex micro organism fungicide is by pale gemma bar
Bacterium (Bacillus pallidus), general Pseudomonas (Pantoea), thermophilic anaerobic bacillus (Thermoanaerobacterium
Aotearoense it is mixed after) fermenting.
2. complex micro organism fungicide according to claim 1, it is characterised in that: the preparation side of the complex micro organism fungicide
Method, comprising the following steps:
Bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus are inoculated in fluid nutrient medium cultivate respectively, then will be contained
Have the culture medium of above-mentioned strain according to strain quantitative proportion be bacillus pallidus: general Pseudomonas: thermophilic anaerobic bacillus=(1-4):
(1-4): the ratio mixing of (1-4).
3. a kind of preparation method of the complex micro organism fungicide of heavy oil development, comprising the following steps:
Bacillus pallidus, general Pseudomonas, thermophilic anaerobic bacillus are inoculated in fluid nutrient medium cultivate respectively, then will be contained
The culture medium for having above-mentioned strain is proportionally bacillus pallidus: general Pseudomonas: thermophilic anaerobic bacillus=(1-4): (1-4): (1-
4) ratio mixing.
4. the preparation method of the complex micro organism fungicide of heavy oil development according to claim 3, it is characterised in that: the liquid
Body culture medium is made of the raw material of following weight percent: the corn syrup of 0.5-2.5%, the vitamin of 0.5-2.5%, 0.1-
0.5% KH2PO4, 0.01-0.1% NaNO3, 0.1-0.2% yeast powder, the benzene of 0.01-0.1%, 0.1-0.2% xanthan
Glue, surplus are water.
5. the preparation method of the complex micro organism fungicide of heavy oil development according to claim 3, it is characterised in that: the liquid
The pH of body culture medium is 7-8.
6. the preparation method of the complex micro organism fungicide of heavy oil development according to claim 3, it is characterised in that: the training
The condition of supporting is in 30-40 DEG C of culture 36-50h.
7. the preparation method of the complex micro organism fungicide of heavy oil development according to claim 3, it is characterised in that: described grey
White bacillus, general Pseudomonas, thermophilic anaerobic bacillus inoculum concentration be respectively 1-5%, 1-10% of fluid nutrient medium weight fraction,
1-5%.
8. the preparation method of the complex micro organism fungicide of heavy oil development according to claim 7, it is characterised in that: described grey
White bacillus, general Pseudomonas, thermophilic anaerobic bacillus inoculum concentration be respectively 1%, 1%, the 3% of fluid nutrient medium weight fraction.
9. application of the complex micro organism fungicide as described in claim 1 in oil recovery.
10. application of the complex micro organism fungicide of heavy oil development according to claim 9 in oil recovery, it is characterised in that:
The complex micro organism fungicide disposably or is in batches injected in oil well.
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Cited By (2)
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| CN111608623A (en) * | 2020-04-27 | 2020-09-01 | 夏文杰 | Biological nano preparation applied to oil and gas resource exploitation |
| CN113736678A (en) * | 2020-05-28 | 2021-12-03 | 中国石油天然气股份有限公司 | Compound microbial agent, preparation method and application thereof |
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| CN111608623A (en) * | 2020-04-27 | 2020-09-01 | 夏文杰 | Biological nano preparation applied to oil and gas resource exploitation |
| CN113736678A (en) * | 2020-05-28 | 2021-12-03 | 中国石油天然气股份有限公司 | Compound microbial agent, preparation method and application thereof |
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Effective date of registration: 20220317 Address after: 230000 8rb18, 2f, building 8, Internet Industrial Park, No. 17, Huayuan Avenue, Baohe Economic Development Zone, Hefei, Anhui Province Patentee after: Anhui chaoming Technology Co.,Ltd. Address before: 236000 No. 582 Huayuan Avenue, Baohe Economic Development Zone, Hefei, Anhui Patentee before: ANHUI LINDE BIOTECHNOLOGY Co.,Ltd. |