CN103060245A - Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof - Google Patents
Compound microorganism bacterium agent of biological coalbed methane prepared by coal bed organic impurities and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of preparing biological coalbed methane by microorganisms, and discloses a compound microorganism bacterium agent of the biological coalbed methane prepared by coal bed organic impurities and an application of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises fermentation broths of four bacteria namely Phanerochaetechrysosporium, Penicilliumchrysogenum, Pseudomonasecpacia and Clostridiumacetobutylium. The compound microorganism bacterium agent disclosed by the invention has strong digestive ability to various celluloses, humus and various carbon-containing macromolecules in the coal bed. A coal added with the compound microorganism bacterium agent can accelerate the enrichment of methanogenus, shorten anaerobic digestion time and improve the output of the biological coalbed methane; and in addition, the synergy effects of the four microorganism strains are superior to the effect of any one, two or three microorganisms.
Description
Technical field
The invention belongs to microorganism and produce biological coal-seam gas field, relate to a kind of complex micro organism fungicide and application thereof that utilizes the coal seam organic impurity to produce biological coal-seam gas.
Background technology
Biological coal-seam gas, be commonly called as gas, that the biological organic impurity that contains in the coal seam is under suitable envrionment conditions, hydrolysis, acidifying and methanation through serial microorganism, and the gas take methane as main component of the physics chemical action of High Temperature High Pressure generation, other has a small amount of ethane, propane and butane, and hydrogen sulfide, carbonic acid gas, nitrogen and aqueous vapor etc., the overwhelming majority comes from biological action, therefore also claims biological coal-seam gas.When biological coal-seam gas concentration between 5%-16%, meet fire and namely will blast, therefore brought grave danger to the colliery safety in production, restricted the coal production force level, threaten miner's life security.But biological coal-seam gas also is a kind of resource simultaneously, is a kind of high-quality, the clean energy, if only the biological coal-seam gas extraction in colliery or wind are discharged among the atmosphere, has then not only wasted resource but also affected environment; If it be can be used as for civilian use or industrial application of fuel, perhaps generating has not only increased the energy, has purified air, has reduced topsoil, can also obtain considerable economic benefit simultaneously.National Development and Reform Committee also clearly proposes " gas is resource and clean energy " and " makes up the Coal Industrial new order of safety, efficient, energy-saving and environmental protection " in " control of coalmine gas with utilize overall plan ".
The biological organic impurity of complicated coal class need to pass through the biotransformations such as hydrolysis, acidifying, methanation and could generate methane, and wherein hydrolysis stage is the rate-limiting step of whole process.Only have complicated organic matter to be hydrolyzed into as could being utilized by the methanogen of original work in the coal seam behind the small molecules of acetic acid, formic acid and so on.Think that in theory as long as satisfy the movable needed environment of methanogen and two conditions of nutritive substance, biological coal-seam gas just can produce in a large number.Produce the coal elemental composition of methane for easily being degraded by microorganisms in the coal seam, colliery, filter out the functional microorganism with efficient degradation ability, utilize microbial process quickly these conversions to be methane gas.Can collect a large amount of methane gass on the one hand, become a kind of energy of high-quality for the productive life needs; On the other hand, effectively solved in the coal seam " source " of gas, for follow-up exploitation work provides safety control.In addition, the present technique volatile and loss composition in the coal of mainly degrading does not reduce or seldom reduces coal quality, does not affect the further use of processing coal.
The U.S. just began to introduce microorganism in the cbm development Practical Project and obtain preferably effect in year in last century 90, and the state such as Canadian, Australian begins to follow up subsequently.China explores, exploits mainly for existing bed gas reservoir at present, the colleges and universities such as China Mining University, the Chinese Academy of Sciences, University of Science ﹠ Technology, Beijing and institute have carried out the research that preliminary microorganism is produced coal-seam gas, being 201210035682.0 and disclosing in " a kind of inoculating microbe volume increase coal-seam gas methods of utilizing " a kind of such as number of patent application utilizes the external source bacterium to activate that the origin bacterium produces methane gas in the coal seam, main anaerobic sludge carries out enrichment culture and obtains compound function yeast, bacterium liquid is added in the coal seam to produce methane again; And for example number of patent application is to disclose a kind of method of utilizing Phanerochaete chrysosporium and substratum degraded coal producing coalbed gas in 201110420196.6 " a kind of microbiological deterioration coal that utilizes is to produce the method for biogas ".Number of patent application is " microbiological degradation technology for coal mine gas " employing methane-oxidizing bacteria injection of 200510069190.3 or is applied to each position, coal seam, degraded gas, and it is dangerous to reduce seam mining.All without the bacterial classification of clear and definite strain number, gained, deficient in stability are tamed to the related microorganism of above-mentioned patent by microorganism used therefor system; Proportioning and act synergistically not quite clear between bacterial strain; Also not to coal-seam gas production process operation intervention.
Summary of the invention
The objective of the invention is the above-mentioned deficiency for prior art, a kind of complex micro organism fungicide is provided.
Another object of the present invention provides this complex micro organism fungicide and transforms the application that organic impurity is produced biological coal-seam gas in the coal seam.
A kind of complex micro organism fungicide, mainly formed by Phanerochaete chrysosporium (Phanerochaete chrysosporium) fermented liquid, penicillium chrysogenum (Penicillium chrysogenum) fermented liquid, pseudomonas cepacia (Pseudomonas cepacia) fermented liquid and acetone-butanol clostridium (Clostridium acetobutylicum) fermented liquid, described 4 kinds of microorganisms are adopted the normal fermentation preparation, more than in 4 kinds of fermented liquids living bacteria count respectively all 10
10Individual/more than the milliliter.Phanerochaete chrysosporium of the present invention, penicillium chrysogenum, pseudomonas cepacia and acetone-butanol clostridium can be commercially available conventional bacterial classification, also can be the bacterial strains according to this area ordinary method separation and purification.The contriver used the above-mentioned bacterial strains preparation complex micro organism fungicide in multiple source in R﹠D process, all can realize purpose of the present invention, realized technique effect of the present invention.
The fermented liquid optimum ratio of 4 kinds of microorganisms is as follows in the described complex micro organism fungicide: Phanerochaete chrysosporium fermented liquid 1 ~ 25 weight part, Penicllium chrysogenum fermented liquid 1 ~ 17 weight part, pseudomonas cepacia fermented liquid 3 ~ 20 weight parts, acetone-butanol clostridia fermentation liquid 1～20 weight part; More than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter; Further optimum ratio is as follows: Phanerochaete chrysosporium fermented liquid 1 ~ 15 weight part, Penicllium chrysogenum fermented liquid 3 ~ 7 weight parts, pseudomonas cepacia fermented liquid 8 ~ 12 weight parts, acetone-butanol clostridia fermentation liquid 5 ~ 14 weight parts, more than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter; Particularly preferably proportioning is as follows: Phanerochaete chrysosporium fermented liquid 6 ~ 11 weight parts, Penicllium chrysogenum fermented liquid 4 ~ 6 weight parts, pseudomonas cepacia fermented liquid 8 ~ 12 weight parts, acetone-butanol clostridia fermentation liquid 7 ~ 12 weight parts, more than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter.
The organic impurity of complex micro organism fungicide of the present invention in the degraded coal seam produces the application in the thing coal-seam gas.Organic impurity in the complex micro organism fungicide degradable of the present invention coal seam, making it becomes the small-molecule substance that can supply methanobacteria utilization in the coal seam, thereby produces the biological coal-seam gas take methane as main component, and this process does not reduce the coal quality.
Utilize complex micro organism fungicide degraded of the present invention coal seam organic impurity to produce the method for biological coal-seam gas, implementing ground, location, coal seam probing microbial inoculum Injection Well and gas recovery well, coal seam geological condition is arranged under the base area, drilling well position, need satisfy microbial inoculum diffusion and the suction of biological coal-seam gas.
Described complex micro organism fungicide degraded coal seam organic impurity is produced the method for biological coal-seam gas, complex micro organism fungicide of the present invention is directly injected the coal seam by the microbial inoculum Injection Well, generation time is 30～45 days, between proliferation period per 3 days with bacterium liquid by Injection Well and gas recovery well circulation primary.By the sampling analysis of bacterium liquid circulation time, can clearly understand methane and carbon dioxide content in flora propagation situation, substrate variation, culture environment variation, the biological coal-seam gas.
Described complex micro organism fungicide degraded coal seam organic impurity is produced the method for biological coal-seam gas, when pH is lower than 6.0, adds NaHCO
3Regulate pH to 7.0; When methane content is higher than 85%, begin from the biological coal-seam gas of gas recovery well extraction.
Described complex micro organism fungicide degraded coal seam organic impurity is produced the method for biological coal-seam gas, after biological coal-seam gas extraction finishes, bacterium liquid is detached the coal seam, does not affect the follow-up exploitation in coal seam.
Application in the organic impurity generation thing coal-seam gas of complex micro organism fungicide of the present invention in the degraded coal seam is not limited to the complex micro organism fungicide degraded of the present invention coal seam organic impurity that utilizes of putting down in writing in the specification sheets and produces the method for biological coal-seam gas, selects to get final product by this area routine techniques.
Beneficial effect:
The present invention filters out the microorganism strains of 4 kinds of excellent performances from have in a large number the microorganism that produces the methane ability, it is carried out organic assembling according to specific proportioning, make complex micro organism fungicide, this complex micro organism fungicide has very strong digestion ability to all kinds of Mierocrystalline celluloses, soil ulmin and all kinds of carbon containing macromole in the coal seam.
Complex micro organism fungicide of the present invention is processed coal, can accelerate the enrichment of methanogen, shorten anaerobic digestion time, a plurality of experimental results show, it is respond well to adopt this complex micro organism fungicide biology degraded coal to produce biological coal-seam gas, and the initial aerogenesis time does not more add complex micro organism fungicide and shortens 50%-68%.
Complex micro organism fungicide of the present invention can significantly improve gas production rate and gas production rate, and gas production rate improves 98%-190% than the blank group, and gas production rate is 7～12 times of blank group.
Composite microbial bacteria of the present invention uses and presents synergistic effect, and the aerogenesis effect was much better than the use of any strain, two strains or three strain bacterium when namely four strain bacterium were used simultaneously.
Complex micro organism fungicide use of the present invention is simple, and only need direct notes to add the coal seam that needs to process and get final product, and long action time.
Embodiment
Below in conjunction with embodiment the present invention is elaborated, among the embodiment for describe in detail all by the preparation of state of the art embodiment 1 fermented liquid
Present embodiment only is described in detail the particular content of invention as an example of Phanerochaete chrysosporium (ATCC20696), penicillium chrysogenum (ATCC10108), pseudomonas cepacia (ATCC21808) and acetone-butanol clostridium (ATCC824) example, does not limit four kinds of microbe-derived selections in the complex micro organism fungicide of the present invention.
(1) above Phanerochaete chrysosporium (ATCC20696), penicillium chrysogenum (ATCC10108), pseudomonas cepacia (ATCC21808) and acetone-butanol clostridium (ATCC824) are activated to respectively separately preservation slant medium, cultivated 16-72 hour for 26-37 ℃.
The preservation of four kinds of microorganisms is as follows with the slant culture based formulas:
Phanerochaete chrysosporium is adopted substratum: potato 100 grams, glucose 5 grams, yeast extract paste 5 grams, peptone 2 grams, agar 15 grams, 1000 milliliters in tap water, pH nature;
Penicillium chrysogenum adopts substratum: glucose 20 grams, peptone 5 grams, wort 100mL, agar 15 grams, 1000 milliliters in tap water, pH nature;
Pseudomonas cepacia is adopted substratum: glucose 10 grams, extractum carnis 2 grams, peptone 3 grams, Sodium Glutamate 5 grams, potassium primary phosphate 1 gram, sal epsom 0.2 gram, Repone K 0.1g, agar 15 grams, 1000 milliliters in tap water, pH=6.5;
Acetone-butanol clostridium bacterial classification adopts substratum: Semen Maydis powder 100 grams, yeast extract paste 3 grams, agar 15 grams, 1000 milliliters in tap water, pH nature.
(2) will activate good bacterial classification is inoculated in respectively separately in the liquid nutrient medium, 26-37 ℃, cultivate 16-24 hour to logarithmic phase except all the other equal 120 rev/mins of acetone-butanol clostridium, 36 ℃ of anaerobism of acetone-butanol clostridium leave standstill to cultivate and obtained seed liquor in 2 days.
The liquid culture based formulas of four kinds of microorganisms is identical with the slant culture based formulas, need not add agar in the process for preparation.
(3) seeding tank is 200 liters, 150 liters of charge amounts, feed intake by above-mentioned 4 kinds of microbial liquid culture medium prescriptions respectively, then 121 ℃ of sterilizations are 20 minutes, add respectively 1.5 after the cooling and go up and state the seed liquor that is cultured to logarithmic phase, culture temperature 28-37 ℃, 120 rev/mins of mixing speed, air flow 1:1.2(and fermentating liquid volume ratio), cultivate 14-18 hour to logarithmic phase, get fermentation seed liquid.36 ℃ of anaerobism of acetone-butanol clostridium leave standstill cultivated 2 days to get fermentation seed liquid.
(4) produce 2000 liters of fermentor tanks, 1500 liters of charge amounts are produced the tank culture medium prescription with the seeding tank substratum, and then 121 ℃ of sterilizations are 20 minutes, add respectively 150 after the cooling and go up and state fermentation seed liquid.The fermentation condition of each bacterial strain is respectively: Phanerochaete chrysosporium, 35 ℃ of fermentation culture temperature, 200 rev/mins of rotating speeds, air flow 1:1.3(and fermentating liquid volume ratio), incubation time 50 hours; Penicillium chrysogenum, 28 ℃ of fermentation culture temperature, 150 rev/mins of rotating speeds, air flow 1:1.2(and fermentating liquid volume ratio), incubation time 48 hours; Pseudomonas cepacia, 30 ℃ of fermentation culture temperature, 150 rev/mins of rotating speeds, air flow 1:1(and fermentating liquid volume ratio), incubation time 24 hours; Acetone-butanol clostridium, 36 ℃ of fermentation culture temperature, anaerobism was cultivated 72 hours.
During fermentation ends, more than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter.
Embodiment 2 utilizes complex micro organism fungicide to process the biological coal-seam gas of Inner Mongol coal anaerobic fermentation
1, four kinds of microbial fermentation solutions of embodiment 1 preparation mix by following weight part and become complex micro organism fungicide: Phanerochaete chrysosporium fermented liquid 8 weight parts, Penicllium chrysogenum fermented liquid 5 weight parts, pseudomonas cepacia fermented liquid 10 weight parts, acetone-butanol clostridia fermentation liquid 7 weight parts;
2, select colliery, Inner Mongol coal 50kg (rapidly sealing behind the on-site sampling), be divided into 5 parts, add respectively in 5 15L anaerobic reaction-pots;
3, add respectively following microbial fermentation solution or each 80ml:a of microbial fermentation liquid mixture in 4 anaerobic reaction-pots: the Phanerochaete chrysosporium fermented liquid, b: Phanerochaete chrysosporium fermented liquid and Penicllium chrysogenum fermented liquid, c: Phanerochaete chrysosporium fermented liquid, Penicllium chrysogenum fermented liquid and pseudomonas cepacia fermented liquid, d: the complex micro organism fungicide of step 1 preparation, remain a water that adds equivalent for the e blank, mix, sealing, keep 35 ℃ of constant temperature anaerobically fermentings, utilized external peristaltic pump circulation stirring once in per 72 hours.
4, extract fermented liquid in per 7 days and measure pH and concentration of substrate.When pH is lower than 6.0, add NaHCO
3Regulate pH to 7.0.
5, time recording gas production rate.5 anaerobic reaction-pot aerogenesis situations are as shown in table 1:
Table 1 complex micro organism fungicide is processed coal and is produced the thing coal-seam gas
Embodiment 3 utilizes complex micro organism fungicide to process the biological coal-seam gas of Gansu coal anaerobic fermentation
1, microorganism among 4 among the embodiment 1 is replaced with following source: Phanerochaete chrysosporium (CICC40719), Penicllium chrysogenum (ACCC32009), pseudomonas cepacia (CICC20699), acetone-butanol clostridium (CGMCC1.135); Method according to embodiment 1 prepares four kinds of microbial fermentation solutions, mixing by following weight part becomes the complex micro organism fungicide I: Phanerochaete chrysosporium fermented liquid 11 weight parts, Penicllium chrysogenum fermented liquid 6 weight parts, pseudomonas cepacia fermented liquid 8 weight parts, acetone-butanol clostridia fermentation liquid 14 weight parts; Mixing by following weight part becomes the complex micro organism fungicide II: Phanerochaete chrysosporium fermented liquid 26 weight parts, Penicllium chrysogenum fermented liquid 20 weight parts, pseudomonas cepacia fermented liquid 25 weight parts, acetone-butanol clostridia fermentation liquid 22 weight parts.
2, select from colliery, Gansu 60kg (rapidly sealing behind the on-site sampling), be divided into 4 parts, add respectively in 4 20L anaerobic reaction-pots;
3, add respectively following microbial fermentation solution or each 120ml:a of microbial fermentation liquid mixture in 4 anaerobic reaction-pots: the complex micro organism fungicide I, b: complex micro organism fungicide II, c: Phanerochaete chrysosporium fermented liquid, remain a water that adds equivalent for the d blank, mix, sealing keeps 35 ℃ of constant temperature anaerobically fermentings, utilizes external peristaltic pump circulation stirring once in per 72 hours.
4, extract fermented liquid in per 7 days and measure pH and concentration of substrate.When pH is lower than 6.0, add NaHCO
3Regulate pH to 7.0.
5, time recording gas production rate.The time recording gas production rate.4 anaerobic reaction-pot aerogenesis situations are as shown in table 2:
Table 2 complex micro organism fungicide is processed coal and is produced the thing coal-seam gas
Embodiment 4
1, the Phanerochaete chrysosporium among the embodiment 1 (ATCC20696) is replaced with Phanerochaete chrysosporium (CICC40719), acetone-butanol clostridium (ATCC824) replaces with acetone-butanol clostridium (CGMCC1.135), method according to embodiment 1 prepares four kinds of microbial fermentation solutions, mixing in following ratio becomes the complex micro organism fungicide I: Phanerochaete chrysosporium fermented liquid 6 weight parts, Penicllium chrysogenum fermented liquid 4 weight parts, pseudomonas cepacia fermented liquid 12 weight parts, acetone-butanol clostridia fermentation liquid 12 weight parts; Mixing by following weight part becomes the complex micro organism fungicide II: Phanerochaete chrysosporium fermented liquid 7 weight parts, Penicllium chrysogenum fermented liquid 6 weight parts, pseudomonas cepacia fermented liquid 2 weight parts, acetone-butanol clostridia fermentation liquid 23 weight parts.
2, select coal 60kg from the colliery, Shanxi (rapidly sealing behind the on-site sampling), be divided into 4 parts, add respectively in 4 20L anaerobic reaction-pots;
3, add respectively following microbial fermentation solution or each 120ml:a of microbial fermentation liquid mixture in 4 anaerobic reaction-pots: the complex micro organism fungicide I, b: complex micro organism fungicide II, c: Phanerochaete chrysosporium fermented liquid, remain a water that adds equivalent for the d blank, mix, sealing keeps 35 ℃ of constant temperature anaerobically fermentings, utilizes external peristaltic pump circulation stirring once in per 72 hours.
4, extract fermented liquid in per 7 days and measure pH and concentration of substrate.When pH is lower than 6.0, add NaHCO
3Regulate pH to 7.0.
5, time recording gas production rate.The time recording gas production rate.4 anaerobic reaction-pot aerogenesis situations are as shown in table 3:
Table 3 complex micro organism fungicide is processed coal and is produced the thing coal-seam gas
Embodiment 5
1, according to selected certain colliery coal seam geological condition, avoid the coal seam zone of fracture, bore Injection Well and gas recovery well in the coal seam continuous band, two well spacing distances are set as 30 meters according to coal seam permeability;
2, four kinds of microbial fermentation solutions of embodiment 1 preparation mix in proportion and become complex micro organism fungicide, inject the coal seam by high pressure from Injection Well, bred 40 days, between proliferation period per 3 days with bacterium liquid by Injection Well and gas recovery well circulation primary.Each circulation time sampling and measuring pH value when pH is lower than 6.0, is added an amount of NaHCO
3Regulate pH to 7.0;
3, after proliferation period finishes, extract the gas sample every day and measure methane content, when methane content is higher than 85%, begin from the biological coal-seam gas of gas production hole extraction.
Claims (8)
1. a complex micro organism fungicide is characterized in that this complex micro organism fungicide mainly is comprised of Phanerochaete chrysosporium (Phanerochaete chrysosporium) fermented liquid, penicillium chrysogenum (Penicillium chrysogenum) fermented liquid, pseudomonas cepacia (Pseudomonas cepacia) fermented liquid and acetone-butanol clostridium (Clostridium acetobutylicum) fermented liquid.
2. complex micro organism fungicide according to claim 1, the fermented liquid proportioning that it is characterized in that 4 kinds of bacteriums in the described complex micro organism fungicide is as follows: Phanerochaete chrysosporium fermented liquid 1 ~ 25 weight part, Penicllium chrysogenum fermented liquid 1 ~ 17 weight part, pseudomonas cepacia fermented liquid 3 ~ 20 weight parts, acetone-butanol clostridia fermentation liquid 1 ~ 20 weight part, more than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter.
3. complex micro organism fungicide according to claim 1, the fermented liquid proportioning that it is characterized in that 4 kinds of bacteriums in the described complex micro organism fungicide is as follows: Phanerochaete chrysosporium fermented liquid 1 ~ 15 weight part, Penicllium chrysogenum fermented liquid 3 ~ 7 weight parts, pseudomonas cepacia fermented liquid 8 ~ 12 weight parts, acetone-butanol clostridia fermentation liquid 5 ~ 14 weight parts, more than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all 10
10Individual/more than the milliliter.
4. the organic impurity of complex micro organism fungicide claimed in claim 1 in the degraded coal seam produces the application in the thing coal-seam gas; Wherein, described biological coal-seam gas is take methane as main component.
5. utilize complex micro organism fungicide degraded claimed in claim 1 coal seam organic impurity to produce the method for biological coal-seam gas, it is characterized in that implementing ground, location, coal seam probing microbial inoculum Injection Well and gas recovery well, coal seam geological condition is arranged under the base area, drilling well position, satisfies respectively the requirement of microbial inoculum diffusion and the suction of biological coal-seam gas.
6. method according to claim 5, it is characterized in that the described complex micro organism fungicide of claim 1 is directly injected the coal seam by the microbial inoculum Injection Well, generation time is 30～45 days, between proliferation period per 3 days with bacterium liquid by microbial inoculum Injection Well and gas recovery well circulation primary.
7. according to claim 5 or 6 described methods, it is characterized in that when pH is lower than 6.0, adding NaHCO
3Regulate pH to 7.0; When methane content is higher than 85%, begin from the biological coal-seam gas of gas recovery well extraction.
8. method according to claim 7 is characterized in that after biological coal-seam gas extraction finishes bacterium liquid being detached the coal seam, does not affect the follow-up exploitation in coal seam.
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| CN105462908A (en) * | 2014-08-29 | 2016-04-06 | 肖栋 | Technology and method for rapid degradation of organic matters by using microbial community in coal seam |
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| CN106948797A (en) * | 2017-04-07 | 2017-07-14 | 中国地质大学（北京） | A kind of method for increasing production coal bed gas |
| CN109458212A (en) * | 2018-11-29 | 2019-03-12 | 中国矿业大学(北京) | A method of it is gathered using methane-oxidizing bacteria processing driving face local gas |
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| CN106948797B (en) * | 2017-04-07 | 2020-02-11 | 中国地质大学（北京） | Method for increasing production of coal bed gas |
| CN109458212A (en) * | 2018-11-29 | 2019-03-12 | 中国矿业大学(北京) | A method of it is gathered using methane-oxidizing bacteria processing driving face local gas |
| CN109458212B (en) * | 2018-11-29 | 2020-06-09 | 中国矿业大学(北京) | Method for treating local gas accumulation of tunneling working face by using methane-oxidizing bacteria |
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