CN105063093A - Method for preparation of coalbed methane from microorganisms - Google Patents
Method for preparation of coalbed methane from microorganisms Download PDFInfo
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- CN105063093A CN105063093A CN201510479848.1A CN201510479848A CN105063093A CN 105063093 A CN105063093 A CN 105063093A CN 201510479848 A CN201510479848 A CN 201510479848A CN 105063093 A CN105063093 A CN 105063093A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a method for preparation of coalbed methane from microorganisms. The method includes the steps of: (1) screening hydrogenogens and acidophilic methanogens; (2) domesticating related bacteria; (3) conducting bacteria enrichment to make a liquid A; (4) adding the liquid A successively according to coalbed methane well on-site parameters; and (5) selecting a main directional drilled hole and two subsidiary directional drilled holes from directional drilled holes of the coalbed methane well to conduct data monitoring. The mixed bacteria fermented biological fracturing fluid has certain sand-carrying ability, wherein the bacteria have hydrogen and methane producing ability, not only can increase the permeability of coal reservoir, increase coalbed gas resources, but also can realize automatic viscosity reduction, and is in favor of flowback. After flowback, the residual hydrogen and methane producing bacteria can continue transformation to the coal reservoir, and can produce endless streams of biogenic coal-bed methane. The method provided by the invention enhances the success rate of dead well resurrection to a large extent, and also the method for preparation of coalbed methane can also be used for biological exploitation of goaf residual coal.
Description
Technical field
The invention belongs to coal bed gas well production technique field, be specifically related to a kind of method utilizing microorganism to produce coal-seam gas.
Background technology
Coal-seam gas is commonly called as " gas ", and its main component is CH4(methane), with coal association, the Unconventional forage be stored in adsorbed state in coal seam, calorific value be the 2-5 of general coal doubly, main component is methane.The calorific value of 1 cubic metre of pure coal-seam gas is equivalent to 1.13kg gasoline, 1.21kg standard coal, its calorific value is suitable with Sweet natural gas, can mix defeated mixed with Sweet natural gas, and very clean after burning, producing any waste gas hardly, is first-class industry, chemical industry, generating and resident living fuel.When coal-seam gas air concentration reaches 5%-16%, meeting naked light will explode, and this is the root of Gas Explosion Accidents in Coal Mines.Coal-seam gas is directly discharged in air, and its Greenhouse effect are about 21 times of carbonic acid gas, extremely strong to environmental destruction.If first exploiting coal bed methane before coal mining, coal mine gas from explosion rate is by reduction by 70% to 85%.The exploitation of coal-seam gas have the effect of achieving many things at one stroke: clear energy sources, and commercialization can produce huge economic benefit.
Generally, the coal-seam gas primary reserves in certain limit and workable reserve are fixing, along with constantly carrying out of mining, and the closing gradually of porous dehiscence gap, coal bed gas well gas production rate can reduce increasingly, until do not have exploitation economic worth even gas production rate be zero, become dried-up well.Dried-up well is brought back to life, economic aspect or in environmental protection all tool be of great significance.
Research proves, coal is a kind of larger molecular organics, and its side chain and functional group can be utilized fermentation finally to generate hydrogen and methane by microorganism, and the rate of permeation of coal seam reservoirs is improved simultaneously.Microbial growth breeding can improve the viscosity of fermented liquid, and along with the decline phase that grows into of bacterium, its viscosity can decline again.
Summary of the invention
The present invention is in order to solve weak point of the prior art, a kind of method utilizing microorganism to produce coal-seam gas is provided, the method can carry out secondary transformation to coal seam reservoirs, and while increase reservoir permeability, improve Coal bed gas project, dried-up well is brought back to life, coal bed gas well is fully utilized, obtain more coal bed gas resource.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: a kind of method utilizing microorganism to produce coal-seam gas, comprises the following steps,
(1), screening hydrogenogens and addicted to sour methanogen: use laboratory culture base HPU-1 and HPU-2, filter out high-biohydrogen bacteria and addicted to sour methanogen;
(2), Related Bacteria is tamed: according to the reservoir environment of well to be rebuilt, enrichment, separation, cultivation, purifying screen hydrogenogens and respectively addicted to sour methanogen, make it the product producing hydrogen and methane bacterium becoming efficient and well reservoir environment to be rebuilt can be adapted to, record its growth curve, cryopreservation;
(3), enrichment of bacterial makes A liquid: the hydrogenogens CQ of laboratory separation and Extraction is added into HPU-1 substratum enrichment 3-6 days, regulates pH to 6.5; And addicted to sour methanogen SSCJW with HPU-2 substratum enrichment 20-30 days, pH to 6.5 will be regulated, two liquid mixing are as A liquid;
(4), successively A liquid is added according to coal bed gas well on-site parameters: entered by A hydraulic pressure in the directional drilling of coal bed gas well after reacting for some time, if gas production rate declines, again using A liquid as adding liquid and pH adjusting agent is together pressed in coal bed gas well, ensure that reservoir liquid pH is between 6-7;
(5), a main directional drilling and two secondary directional drillings are selected in the directional drilling of coal bed gas well, main directional drilling is for carrying microorganism, add culture material, manufacture the living environment that microorganism is applicable to, monitoring according to the later stage constantly carries culture material, ensure effective environment, a secondary directional drilling is for extracting the coal-seam gas produced, the system of accomplishing, take out separately, ensure efficient extraction coal-seam gas, the secondary directional drilling of another one is used for laying monitor, temperature in monitoring coal seam, pH value, monitor the pH value in coal seam constantly, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
Utilize the main directional drilling of means effect of fixed point explosion to make it crack, coal seam in step (5) to increase, for the press-in of microorganism provides precondition.
For ensureing the adaptability of hydrogenogens and methanogen and ground environment in step (1), utilizing mud to carry out acidproof microorganism and screening accordingly.
Screening hydrogenogens and addicted to sour methanogen in step (1), take off because the acid that hydrogenogens produces being divided addicted to sour methanogen, pH is stablized ensure that the activity of hydrogenogens, screening hydrogenogens and act synergistically on coal seam addicted to sour methanogen simultaneously and produce hydrogen and methane, thus improve functioning efficiency.
Selecting with the stage residing for bacterium fluid viscosity and bacterial growth breeding for foundation of the middle A liquid enrichment incubation time of step (3), bacterium fluid viscosity is minimum remains on 27mPas, and growth phase is in logarithmic phase or be about to enter logarithmic phase.
Step (4) if in coal bed gas well be dried-up well, remaining coal bed gas content is little, after press-in A liquid carries out product producing hydrogen and methane for some time, if gas production rate decline continuously and be down to mean level (ML) 50% or following time, A liquid is added by main directional drilling, and temperature pH value etc. in the monitor monitoring coal seam of laying according to secondary directional drilling, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
In step (1), the composition of A liquid is according to peptone-4g; Extractum carnis-3g; Yeast extract paste-1g; NaCl-3g; KH
2pO
4-1.5g; MgSO
47H
2o-0.05g; FeSO
47H
2o-0.05g; Glucose-15g; Cys-0.5g; Hexavitamin (VC, VA, VD2, VB1, VB2, VB6)-2.5g; Ammonium chloride-1g; Three water dipotassium hydrogen phosphate--0.4g; 0.1% resazurin-1ml; Pancreas casein-1g; The proportional arrangement of distilled water-1000ml forms; The pH=6.5 of A liquid, at 120 DEG C of sterilizing 30min after raw material mixing, and adds microbiotic.
In step (5), continuously increase the nutritive substance of microorganism, ensure the living environment of microorganism.
In step (5), produce and extract and separately carry out, ensure that coal-seam gas extracts efficiently.
Step (5) makes in implementation process, the pH value in Real-Time Monitoring coal seam, ensures that the living environment of microorganism reaches optimum.
Adopt technique scheme, the present invention utilizes microbial decomposition coal body, produce hydrogen, methane, the more important thing is the living environment utilizing and provide microorganism suitable, reach and produce efficiently for a long time, the object of extraction coal-seam gas, be a kind of pollution-free, efficiency of the practice is high, the method for coal-seam gas that what the output value was large prepare, waste coal layer is served to the effect of recycling, get a good eye value.
Hydrogenogens and acting synergistically addicted to sour methanogen, can make coal seam and substratum chemical reaction more fully and completely.Composition and the preparation technology of substratum are unique, are added with microbiotic simultaneously, and by culturing sludge, make produce good chemical reaction between microorganism and coal.Adopting fixed point explosion when waterfrac treatment, is 3Mp to 4Mp the pressure that substratum is compressed into.Detection well is set especially, can chemical reaction be wherein detected at any time, thus control subsurface environment.In addition, down-hole extra-nutrition base at any time can be carried out.
In sum, the biological fracturing liquid of the mixed fermentation that the present invention prepares has certain prop-carrying capacity, and bacterium wherein has product producing hydrogen and methane ability, not only can increase coal reservoir fracture system, improve Coal bed gas project and automatic viscosity reduction can be realized, being beneficial to the row of returning.Product producing hydrogen and methane bacterium residual after the row of returning can continue to transform coal seam reservoirs, and produces biogenesis coal-seam gas endlessly.The present invention improves the success ratio that dried-up well brings back to life to a great extent, and this method producing coal-seam gas also can be used for the biology exploitation of the residual coal in worked-out section simultaneously.
Accompanying drawing explanation
Fig. 1 is schema of the present invention;
Fig. 2 is the structural representation of the present invention in borehole operation;
Fig. 3 is chemical reaction schematic diagram of the present invention;
In figure: the main directional drilling of 4-, the secondary directional drilling of 5-, the secondary directional drilling of 6-, 7-monitor.
Embodiment
As depicted in figs. 1 and 2, a kind of method utilizing microorganism to produce coal-seam gas of the present invention, comprises the following steps:
(1), screening hydrogenogens and addicted to sour methanogen: use laboratory culture base HPU-1 and HPU-2, filter out high-biohydrogen bacteria and addicted to sour methanogen;
(2), Related Bacteria is tamed: according to the reservoir environment of well to be rebuilt, enrichment, separation, cultivation, purifying screen hydrogenogens and respectively addicted to sour methanogen, make it the product producing hydrogen and methane bacterium becoming efficient and well reservoir environment to be rebuilt can be adapted to, record its growth curve, cryopreservation;
(3), enrichment of bacterial makes A liquid: the hydrogenogens CQ of laboratory separation and Extraction is added into HPU-1 substratum enrichment 3-6 days, regulates pH to 6.5; And addicted to sour methanogen SSCJW with HPU-2 substratum enrichment 20-30 days, pH to 6.5 will be regulated, two liquid mixing are as A liquid;
(4), successively A liquid is added according to coal bed gas well on-site parameters: entered by A hydraulic pressure in the directional drilling of coal bed gas well after reacting for some time, if gas production rate declines, again using A liquid as adding liquid and pH adjusting agent is together pressed in coal bed gas well, ensure that reservoir liquid pH is between 6-7;
(5), a main directional drilling 4 is selected in the directional drilling of coal bed gas well, secondary directional drilling 5 and secondary directional drilling 6, , main directional drilling 4 is for carrying microorganism, add culture material, manufacture the living environment that microorganism is applicable to, monitoring according to the later stage constantly carries culture material, ensure effective environment, a secondary directional drilling 5 is for extracting the coal-seam gas produced, the system of accomplishing, take out separately, ensure efficient extraction coal-seam gas, the secondary directional drilling 6 of another one is for laying monitor 7, temperature in monitoring coal seam, pH value, monitor the pH value in coal seam constantly, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
Utilize the main directional drilling 4 of means effect of fixed point explosion to make it crack, coal seam in step (5) to increase, for the press-in of microorganism provides precondition.
For ensureing the adaptability of hydrogenogens and methanogen and ground environment in step (1), utilizing mud to carry out acidproof microorganism and screening accordingly.
Screening hydrogenogens and addicted to sour methanogen in step (1), take off because the acid that hydrogenogens produces being divided addicted to sour methanogen, pH is stablized ensure that the activity of hydrogenogens, screening hydrogenogens and act synergistically on coal seam addicted to sour methanogen simultaneously and produce hydrogen and methane, thus improve functioning efficiency.
Selecting with the stage residing for bacterium fluid viscosity and bacterial growth breeding for foundation of the middle A liquid enrichment incubation time of step (3), bacterium fluid viscosity is minimum remains on 27mPas, and growth phase is in logarithmic phase or be about to enter logarithmic phase.
Step (4) if in coal bed gas well be dried-up well, remaining coal bed gas content is little, after press-in A liquid carries out product producing hydrogen and methane for some time, if gas production rate decline continuously and be down to mean level (ML) 50% or following time, A liquid is added by main directional drilling 4, and according to the monitor 7 that secondary directional drilling is laid, temperature pH value etc. in monitoring coal seam, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
In step (1), the composition of A liquid is according to peptone-4g; Extractum carnis-3g; Yeast extract paste-1g; NaCl-3g; KH
2pO
4-1.5g; MgSO
47H
2o-0.05g; FeSO
47H
2o-0.05g; Glucose-15g; Cys-0.5g; Hexavitamin (VC, VA, VD2, VB1, VB2, VB6)-2.5g; Ammonium chloride-1g; Three water dipotassium hydrogen phosphate--0.4g; 0.1% resazurin-1ml; Pancreas casein-1g; The proportional arrangement of distilled water-1000ml forms; The pH=6.5 of A liquid, at 120 DEG C of sterilizing 30min after raw material mixing, and adds microbiotic.
In step (5), continuously increase the nutritive substance of microorganism, ensure the living environment of microorganism.
In step (5), produce and extract and separately carry out, ensure that coal-seam gas extracts efficiently.
Step (5) makes in implementation process, the pH value in Real-Time Monitoring coal seam, ensures that the living environment of microorganism reaches optimum.
Embodiment in the present invention not does any pro forma restriction to shape of the present invention, material, structure etc.; every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (10)
1. utilize microorganism to produce a method for coal-seam gas, it is characterized in that: comprise the following steps,
(1), screening hydrogenogens and addicted to sour methanogen: use laboratory culture base HPU-1 and HPU-2, filter out high-biohydrogen bacteria and addicted to sour methanogen;
(2), Related Bacteria is tamed: according to the reservoir environment of well to be rebuilt, enrichment, separation, cultivation, purifying screen hydrogenogens and respectively addicted to sour methanogen, make it the product producing hydrogen and methane bacterium becoming efficient and well reservoir environment to be rebuilt can be adapted to, record its growth curve, cryopreservation;
(3) enrichment of bacterial makes A liquid: the hydrogenogens CQ of laboratory separation and Extraction is added into HPU-1 substratum enrichment 3-6 days, regulates pH to 6.5; And addicted to sour methanogen SSCJW with HPU-2 substratum enrichment 20-30 days, pH to 6.5 will be regulated, two liquid mixing are as A liquid;
(4), successively A liquid is added according to coal bed gas well on-site parameters: entered by A hydraulic pressure in the directional drilling of coal bed gas well after reacting for some time, if gas production rate declines, again using A liquid as adding liquid and pH adjusting agent is together pressed in coal bed gas well, ensure that reservoir liquid pH is between 6-7;
(5), a main directional drilling and two secondary directional drillings are selected in the directional drilling of coal bed gas well, main directional drilling is for carrying microorganism, add culture material, manufacture the living environment that microorganism is applicable to, monitoring according to the later stage constantly carries culture material, ensure effective environment, a secondary directional drilling is for extracting the coal-seam gas produced, the system of accomplishing, take out separately, ensure efficient extraction coal-seam gas, the secondary directional drilling of another one is used for laying monitor, temperature in monitoring coal seam, pH value, monitor the pH value in coal seam constantly, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
2. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, is characterized in that: utilize the main directional drilling of means effect of fixed point explosion to make it crack, coal seam in step (5) and increase, for the press-in of microorganism provides precondition.
3. a kind of method utilizing microorganism to produce coal-seam gas according to claim 2, is characterized in that: for ensureing the adaptability of hydrogenogens and methanogen and ground environment in step (1), utilizing mud to carry out acidproof microorganism and screening accordingly.
4. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, it is characterized in that: screening hydrogenogens and addicted to sour methanogen in step (1), take off because the acid that hydrogenogens produces being divided addicted to sour methanogen, pH is stablized ensure that the activity of hydrogenogens, screen hydrogenogens and act synergistically on coal seam addicted to sour methanogen simultaneously and produce hydrogen and methane, thus improve functioning efficiency.
5. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, it is characterized in that: in step (3), A liquid enrichment incubation time selectes with the stage residing for bacterium fluid viscosity and bacterial growth breeding as foundation, bacterium fluid viscosity is minimum remains on 27mPas, and growth phase is in logarithmic phase or be about to enter logarithmic phase.
6. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, it is characterized in that: step (4) if in coal bed gas well be dried-up well, remaining coal bed gas content is little, after press-in A liquid carries out product producing hydrogen and methane for some time, if gas production rate decline continuously and be down to mean level (ML) 50% or following time, A liquid is added by main directional drilling, and temperature pH value etc. in the monitor monitoring coal seam of laying according to secondary directional drilling, to supplement the nutritive substance needed for microorganism in time, ensure the efficient living environment of microorganism.
7. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, is characterized in that: in step (1), the composition of A liquid is according to peptone-4g; Extractum carnis-3g; Yeast extract paste-1g; NaCl-3g; KH
2pO
4-1.5g; MgSO
47H
2o-0.05g; FeSO
47H
2o-0.05g; Glucose-15g; Cys-0.5g; Hexavitamin (VC, VA, VD2, VB1, VB2, VB6)-2.5g; Ammonium chloride-1g; Three water dipotassium hydrogen phosphate--0.4g; 0.1% resazurin-1ml; Pancreas casein-1g; The proportional arrangement of distilled water-1000ml forms; The pH=6.5 of A liquid, at 120 DEG C of sterilizing 30min after raw material mixing, and adds microbiotic.
8. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, is characterized in that: the nutritive substance continuously increasing microorganism in step (5), ensures the living environment of microorganism.
9. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, is characterized in that: in step (5), produce and extract and separately carry out, and ensures that coal-seam gas extracts efficiently.
10. a kind of method utilizing microorganism to produce coal-seam gas according to claim 1, is characterized in that: step (5) makes in implementation process, the pH value in Real-Time Monitoring coal seam, ensures that the living environment of microorganism reaches optimum.
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Cited By (10)
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| CN107358858A (en) * | 2017-07-18 | 2017-11-17 | 山西蓝焰煤层气集团有限责任公司 | Coal seam Biogenetic Gas Reservoir process simulation device and experimental method |
| CN107387044A (en) * | 2017-08-22 | 2017-11-24 | 太原理工大学 | A kind of method that biological methane output is improved using coal seam origin fungi |
| CN107387043A (en) * | 2017-08-14 | 2017-11-24 | 西南石油大学 | A kind of method that spontaneous multicomponent gas displacement in coal seam improves coal bed gas recovery ratio |
| WO2018170830A1 (en) * | 2017-03-23 | 2018-09-27 | 陈信平 | Method for increasing production of coal bed gas by injecting high temperature air |
| CN108775240A (en) * | 2018-04-20 | 2018-11-09 | 中国矿业大学(北京) | A kind of method of half-edge coal seam fluidization exploitation |
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| CN112922599A (en) * | 2021-04-08 | 2021-06-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
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| CN114607319A (en) * | 2022-03-28 | 2022-06-10 | 贵州省油气勘查开发工程研究院 | Method for improving coal bed gas yield of multi-branch butt well through intermittent circulating liquid injection |
| CN115124990A (en) * | 2022-06-22 | 2022-09-30 | 重庆大学 | Clean nutrient medium working solution and method for exploiting coal bed gas by using same |
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| CN107358858B (en) * | 2017-07-18 | 2023-05-09 | 山西蓝焰煤层气集团有限责任公司 | Coal bed biological gas reservoir process simulation device and experimental method |
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| CN107387043A (en) * | 2017-08-14 | 2017-11-24 | 西南石油大学 | A kind of method that spontaneous multicomponent gas displacement in coal seam improves coal bed gas recovery ratio |
| CN107387043B (en) * | 2017-08-14 | 2019-10-01 | 西南石油大学 | A kind of method that the spontaneous multicomponent gas displacement in coal seam improves coal bed gas recovery ratio |
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| CN108775240A (en) * | 2018-04-20 | 2018-11-09 | 中国矿业大学(北京) | A kind of method of half-edge coal seam fluidization exploitation |
| CN112922599A (en) * | 2021-04-08 | 2021-06-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
| CN112922599B (en) * | 2021-04-08 | 2022-02-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
| CN113236220A (en) * | 2021-05-31 | 2021-08-10 | 中国矿业大学 | Method for capturing and sealing carbon by utilizing coal bed and producing low-carbon renewable natural gas |
| CN114607319A (en) * | 2022-03-28 | 2022-06-10 | 贵州省油气勘查开发工程研究院 | Method for improving coal bed gas yield of multi-branch butt well through intermittent circulating liquid injection |
| CN114607319B (en) * | 2022-03-28 | 2024-04-05 | 贵州省油气勘查开发工程研究院 | Method for improving coalbed methane yield of multi-branch butt joint well by intermittent circulating liquid injection |
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| CN115124990B (en) * | 2022-06-22 | 2023-05-23 | 重庆大学 | Nutrient medium cleaning working solution and method for mining coalbed methane by using same |
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