CN105533742B - 一种青海三刺多糖组合物及泡腾片剂和用途 - Google Patents
一种青海三刺多糖组合物及泡腾片剂和用途 Download PDFInfo
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Abstract
本发明提供了一种青海三刺多糖组合物，其原料由如下重量配比的组份组成：黄刺多糖10～85份、沙棘多糖8～85份、唐古特白刺多糖3～25份。本发明还提供了基于该组合物的片剂。本发明研究发现，黄刺多糖、沙棘多糖、和白刺多糖单独在体外抗氧化实验中均具有良好的抗氧化活性，但将三者联合使用后，在相同浓度下即可显著提高抗羟自由基、超氧阴离子自由基，以及ABTS自由基的能力，其抗氧化活性明显增强，说明将三种多糖联合使用发挥了协同增效作用。
Description
技术领域
本发明属于保健品领域，具体涉及以黄刺、沙棘、白刺果粉制备所得多糖为原料的片剂， 及其用途。
技术背景
黄刺、唐古特白刺、沙棘(黑刺)这三刺果实均为蒙、藏、维吾尔等少数民族药用与食 用的传统野生浆果，又因其为低矮灌木，且具刺，因此统称为“青海三刺”。上世纪八十年代， 有研究对这三种果实成分进行分析，随之也有青海三刺饮料和面霜问世。目前，对于唐古特 白刺和黑刺，研究和应用十分透彻，而对于三刺果实，其复合产品的成分及功能作用应用仍 较为空白。因此，我们采用青藏高原特色浆果-“青海三刺”为主要原料，开发了三种浆果资 源的同时，又解决了浆果资源的销路，具有一定的经济价值。
目前还未见对青海三刺混合多糖活性及应用的报道。
发明内容
本发明的目的在于提供一种基于青海三刺即黄刺、沙棘和白刺三种浆果制备所得多糖的 泡腾片剂。本发明的另一目的在于提供该泡腾片剂的制备方法和用途。
具体地，本发明提供了一种青海三刺多糖组合物，其原料由如下重量配比的组份组成：
黄刺多糖10～85份、沙棘多糖8～85份、唐古特白刺多糖3～25份。
进一步地，其原料由如下重量配比的组份组成：
黄刺多糖10～13份、沙棘多糖8～15份、唐古特白刺多糖3～10份。
本发明一个具体实施方式中，使用的配比为：黄刺多糖∶沙棘多糖∶白刺多糖＝1∶1∶1。
其中，所述黄刺多糖、沙棘多糖或唐古特白刺多糖，分别是以黄刺、沙棘或唐古特白刺为 原料，经过水提或榨汁后，醇沉制备得到；进一步地，醇沉时乙醇浓度达到60～85％v/v。
本发明还提供了一种青海三刺多糖片剂，它包含上述青海三刺多糖组合物，还包含药学 上常用的辅料。
进一步地，青海三刺多糖组合物的含量为20～85％w/w，优选为20～30％w/w。
更进一步地，所述片剂由含有如下重量配比的原辅料制备而成：
黄刺多糖10～85份、沙棘多糖8～85份、唐古特白刺多糖3～25份；柠檬酸20～30份，碳 酸氢钠20～30份，微晶纤维素8～25份，淀粉1～3份。
进一步地，所述片剂还包括1～6重量份蔗糖，PEG6000 1～4份。
本发明所述片剂，可以为泡腾片。
本发明还提供了上述组合物或上述片剂在制备抗氧化的药物、保健品或食品中的用途。
进一步地，所述药物、保健品或食品是清除ABTS自由基、超氧阴离子自由基或羟自由基
的药物、保健品或食品。
优选地，所述药物、保健品或食品是清除超氧阴离子自由基的药物、保健品或食品。
其中，所述黄刺为小檗科植物直穗小檗Berberis dasystachya Maxim.，沙棘为胡颓子科沙 棘属Hippophae rhamnoides Linn.；唐古特白刺为蒺藜科白刺属Nitrariatangutorum Bobr.。
本发明研究发现，黄刺多糖、沙棘多糖、和白刺多糖单独在体外抗氧化实验中均具有良 好的抗氧化活性，但将三者联合使用后，在相同浓度下即可显著提高抗羟自由基、超氧阴离 子自由基，以及ABTS自由基的能力，其抗氧化活性明显增强(尤其是抗超氧阴离子自由基)， 说明将三种多糖联合使用发挥了协同增效作用。
本发明将青海三刺多糖制成片剂，即便于产品的贮存、运输和包装，同时也提升了口感， 便于各类人群使用。
具体实施方式
实施例1 本发明多糖组合物的制备
取黄刺多糖10份、沙棘多糖15份、唐古特白刺多糖5份，混匀后，即得本发明组合物。
实施例2 本发明多糖组合物的制备
取黄刺多糖12份、沙棘多糖8份、唐古特白刺多糖7份，混匀后，即得本发明组合物。
实施例3 本发明多糖组合物的制备
取黄刺多糖13份、沙棘多糖10份、唐古特白刺多糖3份，混匀后，即得本发明组合物。
本发明中，各多糖可以按照目前已有的技术进行制备，也可以通过购买市售产品获得。 本发明中多糖参照现有技术制备，方法如下：分别取黄刺、沙棘或唐古特白刺，经过水提或 榨汁后，加入乙醇至60～85％v/v，沉淀制备得到。
实施例4 本发明片剂的制备
表1
将青海三刺浆果多糖、与碳源(碳酸氢钠)干混，后加入乙醇湿混，而后制粒；将酸源与微晶纤维素干混，后加入乙醇湿混，并制粒；常温下鼓风挥干乙醇；再加入PEG6000 和淀粉，混匀后压片，即得本发明泡腾片。
体外抗氧化活性实验
(1)材料：青海三多糖：取黄刺、沙棘、白刺果实(干果或鲜果)任意一种，干燥后，粉碎，经过采用水提醇沉法制备得到，由本发明实施例制备而成，所得三种多糖以重量比黄刺多糖∶沙棘多糖∶白刺多糖＝1∶1∶1的比例进行一系列浓度的配制。
(2)实验仪器及试剂：TGL-16G-A型管式离心机，上海安亭科学仪器厂；UV-722N可见紫外分光光度计，上海尼柯有限公司；HD型恒温水浴锅，辽阳市恒温仪器厂。DPPH、 ABTS购于上海晶纯生化科技股份有限公司(阿拉丁)；过硫酸钾、磷酸二氢钠、磷酸氢二钠、 氯化钠、三氯乙酸等试剂均购于山东禹王实业有限公司；超氧阴离子试剂盒、羟自由基试剂 盒均购于南京建成生物科技有限公司。
(3)ABTS清除能力：将ABTS溶解(+H2O)至终浓度为7mmol/L，过硫酸钾配制成2.45mmol/L，将二者溶液混合，放置于黑暗，室温条件下搅拌16h；而后将ABTS混合溶液 用PBS溶液(PH＝7.0)稀释到其吸光值(于734nm处测)为0.70±0.02；配制好的青海三刺 浆果多糖用蒸馏水溶解后稀释至不同浓度；最后将上述不同浓度的样品加入到ABTS混合溶 液中，其样品与ABTS混合溶液比例为1∶20，混合后于室温下孵育6min，于734nm处测定 吸光值。
计算公式：ABTS清除率(％)＝(1-Asample/Acontrol)×100％
其中Acontrol为ABTS混合溶液的吸光值；Asample为ABTS混合溶液中加入样品后测定的 吸光值。
(4)超氧阴离子自由基清除能力和羟自由基均采用南京建成试剂盒检测。配制好的青海 三刺浆果多糖用蒸馏水溶解后稀释至不同浓度。结果见表2～4.
表2 青海三刺多糖与Vc对ABTS的清除能力
| 浓度 | Vc | 沙棘多糖 | 白刺多糖 | 黄刺多糖 | 青海三刺多糖 |
| 8mg/ml | 99.85％ | 88.35％ | 88.91％ | 87.08％ | 90.03％ |
| 16mg/ml | 99.87％ | 90.03％ | 90.45％ | 89.61％ | 91.72％ |
表3 青海三刺多糖和Vc超氧阴离子自由基清除能力
| 浓度 | Vc | 沙棘多糖 | 白刺多糖 | 黄刺多糖 | 青海三刺多糖 |
| 8mg/ml | 99.81％ | 60.51％ | 59.94％ | 54.70％ | 64.14％ |
| 16mg/ml | 99.87％ | 66.65％ | 61.15％ | 59.81％ | 69.64％ |
表4 青海三刺多糖和Vc对羟自由基的清除能力
| 浓度 | Vc | 沙棘多糖 | 白刺多糖 | 黄刺多糖 | 青海三刺多糖 |
| 8mg/ml | 98.45％ | 69.58％ | 88.09％ | 82.10％ | 90.28％ |
| 16mg/ml | 98.54％ | 72.37％ | 89.42％ | 92.70％ | 94.32％ |
上述实验结果表明，黄刺多糖、沙棘多糖、和白刺多糖单独在体外抗氧化实验中均具有 良好的抗氧化活性，但将三者联合使用后，在相同浓度下即可显著提高抗羟自由基、超氧阴 离子自由基，以及ABTS自由基的能力，其抗氧化活性明显增强(尤其是抗超氧阴离子自由 基)，说明将三种多糖联合使用发挥了协同增效作用。
Claims (12)
1.一种青海三刺多糖组合物，其特征在于：其原料由如下重量配比的组份组成：
黄刺多糖10～85份、沙棘多糖8～85份、唐古特白刺多糖3～25份。
2.根据权利要求1所述的青海三刺多糖组合物，其特征在于：其原料由如下重量配比的组份组成：
黄刺多糖10～13份、沙棘多糖8～15份、唐古特白刺多糖3～10份。
3.根据权利要求2所述的青海三刺多糖组合物，其特征在于：黄刺多糖∶沙棘多糖∶唐古特白刺多糖＝1∶1∶1。
4.根据权利要求1或2所述的青海三刺多糖组合物，其特征在于：所述黄刺多糖、沙棘多糖或唐古特白刺多糖，分别是以黄刺、沙棘或唐古特白刺为原料，经过水提或榨汁后，醇沉制备得到。
5.根据权利要求4所述的青海三刺多糖组合物，其特征在于：醇沉时乙醇浓度达到60～85％v/v。
6.一种青海三刺多糖片剂，其特征在于：它包含权利要求1～3任意一项所述的青海三刺多糖组合物，还包含药学上常用的辅料。
7.根据权利要求6所述的青海三刺多糖片剂，其特征在于：所述片剂中，青海三刺多糖组合物的含量为20～85％w/w。
8.根据权利要求7所述的青海三刺多糖片剂，其特征在于：青海三刺多糖组合物的含量为20～30％w/w。
9.根据权利要求6或7所述的青海三刺多糖片剂，其特征在于：所述片剂含有如下重量配比的辅料：
柠檬酸20～30份，碳酸氢钠20～30份，微晶纤维素8～25份，淀粉1～3份。
10.根据权利要求9所述的青海三刺多糖片剂，其特征在于：所述片剂还包括1～6重量份蔗糖，1～4重量份PEG6000。
11.权利要求1～3任意一项所述组合物或权利要求6～10任意一项所述片剂在制备抗氧化的药物、保健品或食品中的用途。
12.根据权利要求11所述的用途，其特征在于：所述药物、保健品或食品是清除ABTS自由基、超氧阴离子自由基或羟自由基的药物、保健品或食品。
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