CN105533682A - Maca active essence with preserved natural activity, preparation method and preparation thereof - Google Patents
Maca active essence with preserved natural activity, preparation method and preparation thereof Download PDFInfo
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- CN105533682A CN105533682A CN201510879241.2A CN201510879241A CN105533682A CN 105533682 A CN105533682 A CN 105533682A CN 201510879241 A CN201510879241 A CN 201510879241A CN 105533682 A CN105533682 A CN 105533682A
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- agate card
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- 230000000694 effects Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 235000000421 Lepidium meyenii Nutrition 0.000 title abstract 9
- 240000000759 Lepidium meyenii Species 0.000 title abstract 9
- 235000012902 lepidium meyenii Nutrition 0.000 title abstract 9
- SUSQOBVLVYHIEX-UHFFFAOYSA-N phenylacetonitrile Chemical compound N#CCC1=CC=CC=C1 SUSQOBVLVYHIEX-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000000605 extraction Methods 0.000 claims abstract description 23
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 238000000926 separation method Methods 0.000 claims abstract description 17
- 239000000341 volatile oil Substances 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 11
- 239000003826 tablet Substances 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 239000002775 capsule Substances 0.000 claims abstract description 5
- 239000006187 pill Substances 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 93
- 239000012141 concentrate Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000008367 deionised water Substances 0.000 claims description 17
- 229910021641 deionized water Inorganic materials 0.000 claims description 17
- 238000004108 freeze drying Methods 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 14
- 238000002203 pretreatment Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 11
- 238000005352 clarification Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 6
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- -1 isothiocyanic acid lipid Chemical class 0.000 claims description 5
- 230000029087 digestion Effects 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 4
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 210000002784 stomach Anatomy 0.000 claims description 3
- 239000005515 coenzyme Substances 0.000 claims description 2
- 230000001079 digestive effect Effects 0.000 claims description 2
- 238000000859 sublimation Methods 0.000 claims description 2
- 230000008022 sublimation Effects 0.000 claims description 2
- 150000002540 isothiocyanates Chemical class 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract description 2
- 239000002552 dosage form Substances 0.000 abstract 1
- MDKCFLQDBWCQCV-UHFFFAOYSA-N benzyl isothiocyanate Chemical compound S=C=NCC1=CC=CC=C1 MDKCFLQDBWCQCV-UHFFFAOYSA-N 0.000 description 10
- 125000004383 glucosinolate group Chemical group 0.000 description 10
- 239000004615 ingredient Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- QMVMMODZGPCSQF-UHFFFAOYSA-N (4-methoxyphenyl)methyl thiocyanate Chemical class COC1=CC=C(CSC#N)C=C1 QMVMMODZGPCSQF-UHFFFAOYSA-N 0.000 description 5
- QAADZYUXQLUXFX-UHFFFAOYSA-N N-phenylmethylthioformamide Natural products S=CNCC1=CC=CC=C1 QAADZYUXQLUXFX-UHFFFAOYSA-N 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 125000004425 isosulfocyanate group Chemical group 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000019633 pungent taste Nutrition 0.000 description 2
- 241000554155 Andes Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000121220 Tricholoma matsutake Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100000734 genotoxic potential Toxicity 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- 239000002893 slag Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a maca active essence with preserved natural activity, a preparation method and preparation thereof. The maca active essence is prepared from fresh maca, frozen maca, and freeze-dried maca. The raw materials are subjected to pretreatment, bionic extraction, separation, concentration, sterilization, and drying to prepare the maca active essence. The maca active essence comprises active components namely isothiocyanate and benzyl cyanide, wherein isothiocyanate accounts for more than 60% or of volatile oil, and benzyl cyanide accounts for less than 30% of volatile oil. The preparation method comprises pretreatment, bionic extraction, separation, concentration, sterilization, and drying. Pharmaceutically acceptable auxiliary materials are added into the maca active essence to prepare preparations in various dosage forms: powder, tablets, capsules, and pills. The whole technology is performed under a cold chain condition, a natural bionic technology based on cold chain is adopted, and the effective components of the product can be naturally and bionically degraded in vitro.
Description
Technical field
The invention belongs to technical field of food deep processing, belong to vegetable active elite processing technique field further, be specifically related to a kind of active elite of agate card retaining natural activity and preparation method thereof and preparation.
Background technology
At present, the product form of agate card is general all dryly plays powder compressing tablet or extract compressing tablet or pulvis, also has the freeze-drying agate card compressing tablet keeping activity, but there is following problem:
1, to dry or to dry the drug activity of agate card poor, and effect activity that there is traditional extract beats the problem of powder compressing tablet not as simple agate card.Further, Chinese medicine such as the interpolation of agate card ginseng or matsutake etc. is just made compound preparation, to strengthen experience effect when taking by businessman.
2, the situation that agate card formulation products ubiquity benzene acetonitrile content is high, benzene acetonitrile and isosulfocyanate are the volatile products in agate card after glucosinolate decomposition, benzene acetonitrile is classified as toxic substance in detecting by edible oil, and U.S. FDA has also pointed out the genotoxic potential of benzene acetonitrile in agate card product specially.Glucosinolate is the principal character effective ingredient in agate card, and the volatile component of agate card is formed primarily of the analyte of glucosinolate.Agate card product on the market, it detects and finds that benzene acetonitrile accounts for more than 60% of Constituents of The Essential Oil, and effective ingredient isothiocyanates only accounts for less than 40%, that is the feature effective ingredient of agate card creates the effective active components of most negative nutrition and small part in decomposable process, and benzene acetonitrile is foods supervision mechanism thinks poisonous or have the composition of negative interaction.
The stimulation nature and flavor of preference agate card when the locals in Andes eats agate card, many people are that making beating is eaten something rare, but the pungent taste of domestic people to agate card is more responsive, is difficult to swallow.Someone by the myrosin deactivation in agate card, allows glucosinolate not easily decompose by the method for sterilization.The agate card product made can be allowed to stimulate as greatly reducing, but also produce isothiocyanates when glucosinolate decomposes simultaneously, this is effective ingredient.If glucosinolate not easily decomposes, so also not easily can generate effective ingredient, its effect activity also can reduce greatly.Therefore, develop a kind of glucosinolate forward and decompose the most of isothiocyanates effective ingredient of generation, and the product of product sense nonirritant acid is very important.
Summary of the invention
The first object of the present invention is to provide a kind of active elite of agate card retaining natural activity; Second object is to provide the preparation method of the active elite of agate card of described reservation natural activity; 3rd object is to provide the preparation of the active elite of agate card of described reservation natural activity.
The first object of the present invention realizes like this, for raw material with fresh agate card, concentrate through pre-treatment, bionic extraction, separation, sterilizing-drying step prepares the active elite of the agate card retaining natural activity, in the active elite of agate card of described reservation natural activity, active ingredient isothiocyanic acid lipid accounts for more than 60% of volatile oil, and benzene acetonitrile is lower than volatile oil 30%.
The second object of the present invention is achieved in that and comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: for subsequent use after defibrination by adding the deionized water of weight ratio 1 ~ 8 times after raw material agate card fresh goods or the cleaning of quick-frozen agate card, when raw material is freeze-drying agate card, then the deionized water adding weight ratio 3 ~ 8 times to soak after 30 minutes defibrination again;
B, bionic extraction: the raw material agate card fresh goods after pre-treatment is inserted in membrane bioreactor dynamic circulation extractor, adds the deionized water of raw material agate card weight ratio 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 45 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of agate card of object reservation natural activity through freeze drying.
The third object of the present invention is achieved in that adding pharmaceutically acceptable auxiliary material in the active elite of the agate card of described reservation natural activity is prepared into powder, tablet, granule, capsule and pill.
Inventor is through animal experiment study, find that fresh agate was stuck in mouse stomach and intestine digested 1 ~ 2 as a child, its decomposition product is optimal, the benzene acetonitrile of small part (< 20%) is only had in the analyte Constituents of The Essential Oil of glucosinolate, and major part is the effective ingredient of isosulfocyanate, illustrate that the natural decomposition process under biomimetic environment is optimal, and illustrate that the natural decomposition of glucosinolate need have the participation of fresh agate card myrosin.
Advantage of the present invention:
1, whole technique completes under cold chain condition, and maximum temperature is no more than 45 DEG C.
2, technique is the natural bionic technique based on cold chain, studying from nature.
3, the product produced is the active elite of agate card, there is no pungent pungent taste, there are sweet taste and the feature taste of agate card polysaccharide, most importantly the analyte of its glucosinolate is that in the volatile oil of main formation, effective ingredient isothiocyanic acid lipid account for more than 60%, only less than 30% benzene acetonitrile, achieve effective ingredient natural bionic forward in vitro and decompose.This feature is just in time contrary with agate card essence sheet on the market and agate card extract.
Accompanying drawing explanation
Fig. 1 is traditional agate card extract Constituents of The Essential Oil chromatogram (embodiment 1);
Fig. 2 is the chromatogram (embodiment 2) of the active elite Constituents of The Essential Oil of agate card of present invention process.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
The active elite of agate card of reservation natural activity of the present invention, for raw material with fresh agate card, concentrate through pre-treatment, bionic extraction, separation, sterilizing-drying step prepares the active elite of the agate card retaining natural activity, in the active elite of agate card of described reservation natural activity, active ingredient isothiocyanic acid lipid accounts for more than 60% of volatile oil, and benzene acetonitrile is lower than 30% of volatile oil.
The preparation method of the active elite of the agate card of reservation natural activity of the present invention, comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: for subsequent use after defibrination by adding the deionized water of weight ratio 1 ~ 8 times after raw material agate card fresh goods or the cleaning of quick-frozen agate card, when raw material is freeze-drying agate card, then the deionized water adding weight ratio 3 ~ 8 times to soak after 30 minutes defibrination again;
B, bionic extraction: the raw material agate card fresh goods after pre-treatment is inserted in membrane bioreactor, adds the deionized water of raw material agate card weight ratio 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 45 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of agate card of object reservation natural activity through freeze drying.
The temperature of defibrination described in step A controls below 20 DEG C.
Membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
Described bionic extraction be by pre-treatment after raw material agate card slurries insert in membrane bioreactor, add the deionized water of raw material agate card slurry weight than 3 ~ 8 times again, adjust ph to 6.5, add digestive ferment, coenzyme and/or aid digestion preparation again, carry out physics bionic extraction 60 ~ 120min in less than 45 DEG C and obtain extract.
Clarification described in step C and separation, impurity removal be adopt filter, the combination of one or more methods of UF membrane or centrifugation.
The temperature that dehydration described in step C concentrates controls below 40 DEG C.
Sublimation temperature < in freeze drying described in D step-18 DEG C.
The preparation of the active elite of the agate card of reservation natural activity of the present invention is add pharmaceutically acceptable auxiliary material in the active elite (pure pulvis) of agate card of described reservation natural activity to be prepared into powder, tablet, granule, capsule and pill.
With concrete case study on implementation, the invention will be further described below:
Embodiment 1(comparative example): (traditional agate card extraction process and detection)
5kg is dried agate card, add water 30 kilograms and be placed in circumfluence distillation tank lixiviate 80 minutes, then obtain extract after filtering clarification, extract is obtained concentrate after vacuum and low temperature reduced pressure concentration, and concentrate obtains 1.2kg dried extract through vacuum and low temperature microwave drying.
Checkout equipment and condition identical with embodiment 2, the testing result of traditional agate card extract is as follows, and as shown in Figure 1, sample total volatile oil content is >=0.074%, and the relative amount of benzene acetonitrile is 75.3662%; The relative amount of benzyl isothiocyanate is 8.7688%; The content of 3-p-methoxybenzeneacetonitrile is 7.5637%; The content of 4-methoxy-benzyl thiocyanates is 1.2017%.
Embodiment 2
With the fresh agate card of 10kg, add 10kg deionized water under cleaning rear less than 20 DEG C cryogenic conditions, then use paste mill grinding, then enter membrane bioreactor, add 40kg deionized water and carry out the biochemical bionic extraction of primary physical.Regulate PH to 6.5 with acetic acid, then add each 0.4 gram of two kinds of Bifidobacterium freeze-dried powders, start to reflect, the reaction time is 75 minutes, and reaction temperature is 38 DEG C.Then clarification, separation, impurity removal and thickening, obtains fresh agate card active elite concentrate 8.6kg.Concentrate is with after low-temperature ultrahigh-pressure sterilizing, and by concentrate quick-frozen immediately to less than-40 DEG C, then freeze drying, obtains 800g dried powder.Directly zero interpolation under high temperature drying condition, makes active agate card elite solid beverage by 1 gram/bag of packaging.
The active elite inspection or testing equipment of agate card and chromatographiccondition
GC:HP5890IIGC. instrument: HP5890IIGC
Column:HP-5,25m × 0.20mm pillar: HP-5,25m × 0.20mm
Carrier:N
2carrier gas: N
2
Oven:100℃to220℃(15min)at4℃/min
Column temperature: 100 DEG C
220 DEG C (15min)
Injection:Split heating rate: 3 DEG C/min
Splitration:50:1. split ratio: 50:1
Detector:FID detector: FID
Injectionvolume:0.2 μ l. sample size: 0.2 μ l.
The testing result of the active elite of the agate card that the present embodiment prepares is as follows, as shown in Figure 2, sample volatile oil content >=0.112%, the content of benzene acetonitrile is 2.6134%, the content of benzyl isothiocyanate is the content of 75.1456%, 4-methoxy-benzyl thiocyanates is 4.8733%.
Embodiment 3 (comparative example)
Pure agate card essence sheet (not adding other Chinese medicinal ingredients) of certain brand purchased from commercial type, with the analytical method same analysis Constituents of The Essential Oil with example 2.
Result is: sample volatile oil content >=0.167%, and wherein benzene acetonitrile accounts for 77.6927%, and benzyl isothiocyanate accounts for 15.5481%, 3-p-methoxybenzeneacetonitrile and accounts for 5.1551%, 4-methoxy-benzyl thiocyanates and account for 0.8435%.
Embodiment 4
With 10kg freeze-drying agate card, the 50kg deionized water that adds water soaks 30 minutes, fiberizer is entered after the water suction of freeze-drying sheet is saturated, again grinding slurry and slag are put into membrane bioreactor simultaneously, add 200kg deionized water, carry out reaction extraction 80 minutes, extract centrifugation after filtering obtains clarifying extract.The quick-frozen immediately after less than 40 DEG C low temperature are concentrated of clarification extract, temporarily to store, concentrate ice cube obtains xeraphium after freeze drying.Xeraphium adds a small amount of compressing tablet desired additives and makes active agate card elite tablet.
Identical with the equipment of embodiment 2 and testing conditions, the testing result of the active elite of the agate card that the present embodiment prepares is as follows: the content of benzene acetonitrile is 12.667%, the content of benzyl isothiocyanate is the content of 61.5421%, 4-methoxy-benzyl thiocyanates is 1.9812%.
Embodiment 5
Thaw with the quick-frozen of 10kg quick-frozen agate card, automatically after cleaning with agate card cleaning machine, Xia High Temperature Refrigeratory Huan Jing, ﹙ < 8 DEG C ﹚ cuts into slices, add 10kg deionized water defibrination, slurry after defibrination is put into membrane bioreactor, add 70kg deionized water again, at 40 DEG C of temperature, carry out reaction extract, fiber hydrolase and the proteolytic enzyme of weight 0.2 gram is added again in leaching process, reaction extraction time is 45 minutes, after reaction extraction terminates, extract obtains 10kg concentrate through concentrated, concentrate is quick-frozen immediately, 880g agate card elite freeze-dried powder is obtained again through freeze drying.Freeze-dried powder adds the appropriate appropriate additive promoting mouthfeel and makes active agate card elite alternative tea by 1 gram/bag of packaging.
Identical with the checkout equipment of embodiment 2 and testing conditions, the testing result of the active elite of the agate card that the present embodiment prepares is as follows: the content of benzene acetonitrile is 9.6311%, the content of benzyl isothiocyanate is the content of 63.8422%, 4-methoxy-benzyl thiocyanates is 3.7674%.
Embodiment 6
The active elite of the agate card that Example 1 prepares adds pharmaceutically acceptable auxiliary material and is prepared into powder.
Embodiment 7
The active elite of the agate card that Example 2 prepares adds pharmaceutically acceptable auxiliary material and is prepared into tablet.
Embodiment 8
The active elite of the agate card that Example 3 prepares adds pharmaceutically acceptable auxiliary material and is prepared into granule.
Embodiment 9
The active elite of the agate card that Example 4 prepares adds pharmaceutically acceptable auxiliary material and is prepared into capsule.
Embodiment 10
The active elite of the agate card that Example 5 prepares adds pharmaceutically acceptable auxiliary material and is prepared into pill.
Claims (9)
1. one kind retains the active elite of agate card of natural activity, it is characterized in that with fresh agate card or freeze-drying agate card for raw material, concentrate through pre-treatment, bionic extraction, separation, sterilizing-drying step prepares the active elite of the agate card retaining natural activity, in the active elite of agate card of described reservation natural activity, active ingredient isothiocyanic acid lipid accounts for volatile oil more than 60%, and benzene acetonitrile is lower than 30% of volatile oil.
2. a preparation method for the active elite of the agate card of reservation natural activity according to claim 1, is characterized in that comprising that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: for subsequent use after defibrination by adding the deionized water of weight ratio 1 ~ 8 times after raw material agate card fresh goods or the cleaning of quick-frozen agate card, when raw material is freeze-drying agate card, then the deionized water adding weight ratio 3 ~ 8 times to soak after 30 minutes defibrination again;
B, bionic extraction: the raw material agate card fresh goods after pre-treatment is inserted in membrane bioreactor dynamic circulation extractor, adds the deionized water of raw material agate card weight ratio 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 45 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of agate card of object reservation natural activity through freeze drying.
3. preparation method according to claim 2, is characterized in that the temperature of defibrination described in step A controls below 20 DEG C.
4. preparation method according to claim 2, is characterized in that the membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
5. preparation method according to claim 2, it is characterized in that described bionic extraction be by pre-treatment after raw material agate card slurries insert in the dynamic circulation extractor of membrane bioreactor, add the deionized water of raw material agate card slurry weight than 3 ~ 10 times again, adjust ph to 6.5, add digestive ferment, coenzyme and/or aid digestion preparation again, carry out bionic extraction 60 ~ 120min in less than 45 DEG C and obtain extract.
6. preparation method according to claim 2, it is characterized in that the clarification described in step C and separation, impurity removal be adopt filter, the combination of one or more methods of UF membrane or centrifugation.
7. preparation method according to claim 2, is characterized in that the temperature that the dehydration described in step C concentrates controls below 40 DEG C.
8. preparation method according to claim 2, is characterized in that the sublimation temperature <-18 DEG C in the freeze drying described in D step.
9. a preparation for the active elite of the agate card of reservation natural activity according to claim 1, is characterized in that adding pharmaceutically acceptable auxiliary material in the active elite of the agate card of described reservation natural activity is prepared into powder, tablet, granule, capsule and pill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510879241.2A CN105533682B (en) | 2015-12-05 | 2015-12-05 | A kind of Maca activity essence and preparation method thereof retaining natural activity and preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510879241.2A CN105533682B (en) | 2015-12-05 | 2015-12-05 | A kind of Maca activity essence and preparation method thereof retaining natural activity and preparation |
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| CN103040896A (en) * | 2013-01-06 | 2013-04-17 | 云南圣草峰生物科技有限公司 | Preparation method of maca extractive |
| CN104055183A (en) * | 2014-06-24 | 2014-09-24 | 方国胜 | Health beverage containing Lepidium peruvianum and production method thereof |
| CN104382975A (en) * | 2014-10-24 | 2015-03-04 | 昆明朗盛生物科技有限公司 | Preparation method and application of Maca extractive |
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| CN103040896A (en) * | 2013-01-06 | 2013-04-17 | 云南圣草峰生物科技有限公司 | Preparation method of maca extractive |
| CN104055183A (en) * | 2014-06-24 | 2014-09-24 | 方国胜 | Health beverage containing Lepidium peruvianum and production method thereof |
| CN104382975A (en) * | 2014-10-24 | 2015-03-04 | 昆明朗盛生物科技有限公司 | Preparation method and application of Maca extractive |
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| Title |
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| 杨义芳等: "《中药提取分离新技术》", 31 December 2009, 化学工业出版社 * |
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