CN105533682B - A kind of Maca activity essence and preparation method thereof retaining natural activity and preparation - Google Patents
A kind of Maca activity essence and preparation method thereof retaining natural activity and preparation Download PDFInfo
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- CN105533682B CN105533682B CN201510879241.2A CN201510879241A CN105533682B CN 105533682 B CN105533682 B CN 105533682B CN 201510879241 A CN201510879241 A CN 201510879241A CN 105533682 B CN105533682 B CN 105533682B
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- 240000000759 Lepidium meyenii Species 0.000 title claims abstract description 95
- 235000000421 Lepidium meyenii Nutrition 0.000 title claims abstract description 95
- 235000012902 lepidium meyenii Nutrition 0.000 title claims abstract description 95
- 230000000694 effects Effects 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- SUSQOBVLVYHIEX-UHFFFAOYSA-N phenylacetonitrile Chemical compound N#CCC1=CC=CC=C1 SUSQOBVLVYHIEX-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 239000000341 volatile oil Substances 0.000 claims abstract description 17
- 238000000926 separation method Methods 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000002203 pretreatment Methods 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 11
- 238000004108 freeze drying Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- -1 isothiocyanic acid lipid Chemical class 0.000 claims abstract description 7
- 239000003826 tablet Substances 0.000 claims abstract description 7
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002775 capsule Substances 0.000 claims abstract description 5
- 239000008187 granular material Substances 0.000 claims abstract description 5
- 239000006187 pill Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
- 239000012141 concentrate Substances 0.000 claims description 14
- 239000012528 membrane Substances 0.000 claims description 11
- 239000012535 impurity Substances 0.000 claims description 6
- 238000005352 clarification Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 230000018044 dehydration Effects 0.000 claims description 4
- 238000006297 dehydration reaction Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 3
- 238000000859 sublimation Methods 0.000 claims description 2
- 230000008022 sublimation Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 11
- 238000000338 in vitro Methods 0.000 abstract description 2
- MDKCFLQDBWCQCV-UHFFFAOYSA-N benzyl isothiocyanate Chemical compound S=C=NCC1=CC=CC=C1 MDKCFLQDBWCQCV-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 125000004383 glucosinolate group Chemical group 0.000 description 9
- 238000000354 decomposition reaction Methods 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 239000002002 slurry Substances 0.000 description 6
- QAADZYUXQLUXFX-UHFFFAOYSA-N N-phenylmethylthioformamide Natural products S=CNCC1=CC=CC=C1 QAADZYUXQLUXFX-UHFFFAOYSA-N 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 238000000227 grinding Methods 0.000 description 4
- QMVMMODZGPCSQF-UHFFFAOYSA-N (4-methoxyphenyl)methyl thiocyanate Chemical class COC1=CC=C(CSC#N)C=C1 QMVMMODZGPCSQF-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 150000002540 isothiocyanates Chemical class 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000019633 pungent taste Nutrition 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 125000004217 4-methoxybenzyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1OC([H])([H])[H])C([H])([H])* 0.000 description 1
- 241000554155 Andes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- JFJXNLPVZZSOMD-UHFFFAOYSA-N COC1=CC=C(C[S])C=C1 Chemical compound COC1=CC=C(C[S])C=C1 JFJXNLPVZZSOMD-UHFFFAOYSA-N 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- NMOJAXCSURVGEY-UHFFFAOYSA-N N#CC#N.[S] Chemical compound N#CC#N.[S] NMOJAXCSURVGEY-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000121220 Tricholoma matsutake Species 0.000 description 1
- 239000011805 ball Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- XLJMAIOERFSOGZ-UHFFFAOYSA-M cyanate Chemical compound [O-]C#N XLJMAIOERFSOGZ-UHFFFAOYSA-M 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100000734 genotoxic potential Toxicity 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 125000004425 isosulfocyanate group Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000008164 mustard oil Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 150000003567 thiocyanates Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of Maca activity essence for retaining natural activity and preparation method thereof and preparations.The Maca activity essence of the reservation natural activity is using fresh Maca, quick-frozen Maca or freeze-drying Maca as raw material, the Maca activity essence for retaining natural activity is prepared through pre-treatment, bionic extraction, separation concentration, sterilizing-drying step, effective component isothiocyanic acid lipid accounts for 60% of volatile oil or more in the Maca activity essence of the reservation natural activity, and benzene acetonitrile is lower than volatile oil 30%.Method includes pre-treatment, bionic extraction, separation concentration, sterilizing-drying step.Preparation is prepared into powder, tablet, granule, capsule and pill for pharmaceutically acceptable auxiliary material is added in the Maca activity essence of the reservation natural activity.The entire technique of the present invention is completed under the conditions of cold chain, the natural bionic technique based on cold chain, studying from nature.The product produced realizes the natural bionic of effective ingredient in vitro and decomposes.
Description
Technical field
The invention belongs to technical field of food deep processing, further belong to vegetable active essence processing technique field, specifically
It is related to Maca activity essence of a kind of reservation natural activity and preparation method thereof and preparation.
Background technique
Currently, generally all powder tabletting or extract tabletting or pulvis are played in drying to the product form of Maca, also there is holding activity
The tabletting of freeze-drying Maca, but there are the following problems:
1, the drug activity for drying or drying Maca is poor, and there is the effect of traditional extract activity and be not so good as simple Ma
The problem of card plays powder tabletting.In addition, just compound preparation is made in the Maca addition Chinese medicines such as ginseng or matsutake by businessman, taken with enhancing
When experience effect.
2, the high situation of the generally existing benzene acetonitrile content of Maca formulation products, benzene acetonitrile and isosulfocyanate are in Maca
Glucosinolate decompose after volatile products, benzene acetonitrile is classified as toxic substance in edible oil detection, U.S. FDA also specially prompts
The genotoxic potential of benzene acetonitrile in Maca product.Glucosinolate is the main feature effective ingredient in Maca, the volatility of Maca
Composition is mainly made of the decomposition product of glucosinolate.Maca product on the market, detection discovery benzene acetonitrile account for Constituents of The Essential Oil
60% or more, and effective ingredient isothiocyanates is only accounted for less than 40%, that is to say, that the feature effective ingredient of Maca was being decomposed
Most negative nutrition and least a portion of effective active components are produced in journey, and benzene acetonitrile is that foods supervision mechanism thinks
Composition that is toxic or having negative interaction.
The stimulation nature and flavor of preference Maca when the locals in Andes eats Maca, many people are that mashing is eaten it raw, but domestic people
It is more sensitive to the pungent taste of Maca, it is difficult to swallow.The method of someone's sterilization inactivates the myrosin in Maca, allows mustard oil
Glycosides is not easily decomposed.The stimulation of Maca product made of can allowing also generates different sulphur cyanogen when glucosinolate decomposition to greatly reduce simultaneously
Acid esters, this is effective ingredient.If glucosinolate is not easily decomposed, also it is not easy that effective ingredient, efficacy activity can be generated
It will be greatly reduced.Therefore, a kind of most of isothiocyanates effective ingredient of glucosinolate forward direction decomposition generation, and product sense are developed
The product of nonirritant acid is very important.
Summary of the invention
The first object of the present invention is to provide a kind of Maca activity essence for retaining natural activity；Second purpose is to mention
For the preparation method of the Maca activity essence of the reservation natural activity；Third is designed to provide the reservation and naturally lives
The preparation of the Maca activity essence of property.
The first object of the present invention is achieved in that using fresh Maca as raw material, through pre-treatment, bionic extraction, is divided
The Maca activity essence for retaining natural activity, the Ma of the reservation natural activity are prepared from concentration, sterilizing-drying step
Block effective component isothiocyanic acid lipid in active essence and account for 60% of volatile oil or more, benzene acetonitrile is lower than volatile oil 30%.
The second object of the present invention is achieved in that including pre-treatment, bionic extraction, separation concentration, sterilizing-drying step
Suddenly, it specifically includes:
A, pre-treatment: 1 ~ 8 times of weight ratio of deionized water will be added through grinding after raw material Maca fresh goods or the cleaning of quick-frozen Maca
It is spare after slurry, when raw material is freeze-drying Maca, then defibrination again is added behind deionized water immersion 30 minutes of 3 ~ 8 times of weight ratio；
B, bionic extraction: the raw material Maca fresh goods after pre-treatment is placed in membrane bioreactor dynamic circulation extractor,
The deionized water of 3 ~ 10 times of raw material Maca weight ratio is added, obtains extracting solution in 45 DEG C or less 60 ~ 120min of progress bionic extraction；
C, separation concentration: extracting solution is clarified, dehydration is concentrated to get concentrate after separation, impurity removal；
D, sterilizing-drying: by concentrate after low temperature sterilization quick-frozen to -20 DEG C hereinafter, freeze-dried again obtain object
Retain the Maca activity essence of natural activity.
The third object of the present invention, which is achieved in that in the Maca activity essence of the reservation natural activity, is added medicine
Acceptable auxiliary material is prepared into powder, tablet, granule, capsule and pill on.
Inventor has found that fresh Maca digested 1 ~ 2 as a child in Mouse Stomach enteral, decomposition product through animal experiment study
It is optimal, the only benzene acetonitrile of small part (< 20%) in the decomposition product Constituents of The Essential Oil of glucosinolate, and most of is different
The effective ingredient of thiocyanate ester illustrates that the natural decomposition process under biomimetic environment is optimal, and illustrates glucosinolate
Natural decomposition need to have the participation of fresh Maca myrosin.
Advantages of the present invention:
1, entire technique is completed under the conditions of cold chain, and maximum temperature is no more than 45 DEG C.
2, technique is the natural bionic technique based on cold chain, studying from nature.
3, the product produced is the active essence of Maca, without pungent pungent taste, there is the sweet taste and feature of Maca polysaccharide
Taste, effective ingredient isothiocyanic acid lipid accounts for 60% in the volatile oil most importantly constituted based on the decomposition product of its glucosinolate
More than, only less than 30% benzene acetonitrile, realizes the natural bionic forward direction of effective ingredient in vitro and decompose.This feature is just
With on the market Maca essence piece and Maca extract it is opposite.
Detailed description of the invention
Fig. 1 is traditional Maca extract Constituents of The Essential Oil chromatogram (embodiment 1)；
Fig. 2 is the chromatogram (embodiment 2) of the Maca activity essence Constituents of The Essential Oil of present invention process.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention is further illustrated, but is not subject in any way to the present invention
Limitation, based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
The Maca activity essence of the present invention for retaining natural activity through pre-treatment, is imitated using fresh Maca as raw material
The Maca activity essence for retaining natural activity is prepared in raw extracting and developing concentration, sterilizing-drying step, and the reservation is natural
Effective component isothiocyanic acid lipid accounts for 60% of volatile oil or more in active Maca activity essence, and benzene acetonitrile is lower than volatile oil
30%。
It is of the present invention retain natural activity Maca activity essence preparation method, including pre-treatment, bionic extraction,
Separation concentration, sterilizing-drying step, specifically include:
A, pre-treatment: 1 ~ 8 times of weight ratio of deionized water will be added through grinding after raw material Maca fresh goods or the cleaning of quick-frozen Maca
It is spare after slurry, when raw material is freeze-drying Maca, then defibrination again is added behind deionized water immersion 30 minutes of 3 ~ 8 times of weight ratio；
B, by the raw material Maca fresh goods merging membrane bioreactor after pre-treatment, raw material Maca weight bionic extraction: is added
Amount compares 3 ~ 10 times of deionized water, obtains extracting solution in 45 DEG C or less 60 ~ 120min of progress bionic extraction；
C, separation concentration: extracting solution is clarified, dehydration is concentrated to get concentrate after separation, impurity removal；
D, sterilizing-drying: by concentrate after low temperature sterilization quick-frozen to -20 DEG C hereinafter, freeze-dried again obtain object
Retain the Maca activity essence of natural activity.
The temperature of defibrination described in step A is controlled at 20 DEG C or less.
Membrane bioreactor described in step B includes pot type and channel-type membrane bioreactor, to simulate stomach and enteron aisle
Structural form and digestion mode.
The bionic extraction is to add raw material in the raw material Maca slurries merging membrane bioreactor after pre-treatment
Deionized water of the Maca slurry weight than 3 ~ 8 times, adjusting pH value to 6.5, adds digestive ferment, coenzyme and/or aid digestion preparation,
Extracting solution is obtained in 45 DEG C or less 60 ~ 120min of progress physics bionic extraction.
Clarification described in step C and separation, impurity removal be use filtering, UF membrane or centrifuge separation it is one or two kinds of with
The combination of upper method.
The temperature of dehydration concentration described in step C is controlled at 40 DEG C or less.
< -18 DEG C of sublimation temperature in freeze-drying described in D step.
The preparation of the Maca activity essence of the present invention for retaining natural activity is the Ma of the reservation natural activity
Block the middle pharmaceutically acceptable auxiliary material that is added of active essence (pure pulvis) and is prepared into powder, tablet, granule, capsule and ball
Agent.
Case is embodied, the invention will be further described below:
Embodiment 1(comparative example): (traditional Maca extraction process and detection)
5kg is dried into Maca, adds 30 kilograms of water to be placed in circumfluence distillation tank and extracts 80 minutes, then after filtering clarification
To extracting solution, extracting solution is obtained into concentrate after vacuum and low temperature is concentrated under reduced pressure, concentrate is obtained through vacuum and low temperature microwave drying
1.2kg dried extract.
Detection device and condition are same as Example 2, and the testing result of traditional Maca extract is as follows, as shown in Fig. 1,
Sample total volatile oil content is >=0.074%, and the relative amount of benzene acetonitrile is 75.3662%；The relative amount of benzyl isothiocyanate
It is 8.7688%；The content of 3- p-methoxybenzeneacetonitrile is 7.5637%；The content of 4- methoxy-benzyl thiocyanates is 1.2017%.
Embodiment 2
With the fresh Maca 10kg, after cleaning under 20 DEG C or less cryogenic conditions plus 10kg deionized water, then with paste mill grinding, so
Enter membrane bioreactor afterwards, 40kg deionized water is added to carry out primary physical biochemistry bionic extraction.PH to 6.5 is adjusted with acetic acid,
Again plus each 0.4 gram of two kinds of Bifidobacterium freeze-dried powders, start to be reflected, the reaction time is 75 minutes, and reaction temperature is 38 DEG C.So
Clarification, separation, impurity removal and thickening afterwards, obtain fresh Maca activity essence concentrate 8.6kg.Concentrate is gone out with low-temperature ultrahigh-pressure
After bacterium, by quick-frozen immediately to -40 DEG C of concentrate hereinafter, being then freeze-dried, 800g dried powder is obtained.Directly in high temperature drying
Under the conditions of zero addition, active Maca essence solid beverage is made by 1 gram/bag of packaging.
Maca activity essence inspection or testing equipment and chromatographiccondition
GC:HP 5890II GC. instrument: HP 5890II GC
Column:HP-5,25m × 0.20mm pillar: HP-5,25m × 0.20mm
Carrier: N2Carrier gas: N2
Oven:100℃ to 220℃ (15min) at 4℃/ min
Column temperature: 100 DEG C220℃ (15min)
Injection:Split heating rate: 3 DEG C/min
Split ration:50:1. split ratio: 50:1
Detector:FID detector: FID
Injection volume:0.2 μ l. sample volume: 0.2 μ l.
The testing result for the Maca activity essence that the present embodiment is prepared is as follows, as shown in Fig. 2, sample volatile oil
Content >=0.112%, the content of benzene acetonitrile are 2.6134%, and the content of benzyl isothiocyanate is 75.1456%, 4- methoxy-benzyl
The content of thiocyanates is 4.8733%.
Embodiment 3 (comparative example)
The pure Maca essence piece of certain brand (being not added with other Chinese medicinal ingredients) purchased from commercial type, with the analysis with example 2
Method same analysis Constituents of The Essential Oil.
As a result are as follows: sample volatile oil content >=0.167%, wherein benzene acetonitrile accounts for 77.6927%, and benzyl isothiocyanate accounts for
15.5481%, 3- p-methoxybenzeneacetonitrile account for 5.1551%, 4- methoxy-benzyl thiocyanates and account for 0.8435%.
Embodiment 4
Maca piece is lyophilized with 10kg, water 50kg deionized water is added to impregnate 30 minutes, enters mill after freeze-drying tablet water suction saturation
Pulp grinder, then grinding slurry and slag are put into membrane bioreactor simultaneously, add 200kg deionized water, carries out reaction and extract 80 minutes, mention
Liquid is taken to be centrifugally separating to obtain clarification extracting solution after filtering.It is quick-frozen immediately after 40 DEG C or less low temperature are concentrated to clarify extracting solution, with
Just temporarily storage, obtains xeraphium after concentrate ice cube is freeze-dried.Additive needed for xeraphium adds a small amount of tabletting is made
Active Maca essence tablet.
It is identical as the equipment of embodiment 2 and testing conditions, the detection knot for the Maca activity essence that the present embodiment is prepared
Fruit is as follows: the content of benzene acetonitrile is 12.667%, and the content of benzyl isothiocyanate is 61.5421%, 4- methoxy-benzyl thiocyanic acid
The content of ester is 1.9812%.
Embodiment 5
With the quick-frozen defrosting in the quick-frozen Maca 10kg, after being cleaned automatically with Maca cleaning machine, the 8 DEG C ﹚ of ﹙ < at High Temperature Refrigeratory Huan Jing
Slice, adds 10kg deionized water defibrination, and the slurry after defibrination is put into membrane bioreactor, then plus 70kg deionized water, 40
Reaction extraction is carried out at a temperature of DEG C, 0.2 gram of weight of fiber hydrolase and proteolytic enzyme are added in extraction process, and reaction mentions
Taking the time is 45 minutes, and after reaction is extracted, extracting solution is concentrated to obtain 10kg concentrate, and concentrate is quick-frozen immediately, then passes through
Freeze-drying obtains the Maca 880g essence freeze-dried powder.The appropriate appropriate additive for promoting mouthfeel of freeze-dried powder addition is by 1 gram/bag of packaging
Active Maca essence alternative tea is made.
It is identical as the detection device of embodiment 2 and testing conditions, the inspection for the Maca activity essence that the present embodiment is prepared
It is as follows to survey result: the content of benzene acetonitrile is 9.6311%, and the content of benzyl isothiocyanate is 63.8422%, 4- methoxy-benzyl sulphur
The content of cyanate is 3.7674%.
Embodiment 6
The Maca activity essence that Example 1 is prepared is added pharmaceutically acceptable auxiliary material and is prepared into powder.
Embodiment 7
The Maca activity essence that Example 2 is prepared is added pharmaceutically acceptable auxiliary material and is prepared into tablet.
Embodiment 8
The Maca activity essence that Example 3 is prepared is added pharmaceutically acceptable auxiliary material and is prepared into granule.
Embodiment 9
The Maca activity essence that Example 4 is prepared is added pharmaceutically acceptable auxiliary material and is prepared into capsule.
Embodiment 10
The Maca activity essence that Example 5 is prepared is added pharmaceutically acceptable auxiliary material and is prepared into pill.
Claims (3)
1. it is a kind of retain natural activity Maca activity essence, it is characterised in that be using fresh Maca or freeze-drying Maca as raw material,
It is prepared through pre-treatment, bionic extraction, separation concentration, sterilizing-drying step, entire technique is completed under the conditions of cold chain, highest
Temperature is no more than 45 DEG C:
A, pre-treatment: by raw material Maca fresh goods or freeze-drying Maca cleaning after be added 1 ~ 8 times of weight ratio deionized water 20 DEG C with
It is lower spare after defibrination, when raw material is freeze-drying Maca, then it is added behind deionized water immersion 30 minutes of 3 ~ 8 times of weight ratio again 20
DEG C or less defibrination；
B, by the raw material merging membrane bioreactor dynamic circulation extractor after pre-treatment, raw material Maca bionic extraction: is added
The deionized water that 3 ~ 10 times of weight ratio obtains extracting solution in 45 DEG C or less 60 ~ 120min of progress bionic extraction；
C, separation concentration: extracting solution is clarified, dehydration is concentrated to get concentrate after separation, impurity removal, and the temperature control for being dehydrated concentration exists
40 DEG C or less；
D, sterilizing-drying: by concentrate after low temperature sterilization quick-frozen immediately to -20 DEG C hereinafter, again < -18 DEG C of sublimation temperature freezing
It is dried to obtain the Maca activity essence that object retains natural activity, effective component isothiocyanic acid lipid accounts in the activity essence of Maca
60% or more volatile oil, benzene acetonitrile are lower than the 30% of volatile oil.
2. according to claim 1 retain natural activity Maca activity essence, it is characterised in that described in step C clarification and
Separation, impurity removal is the combination using one or more kinds of methods of filtering, UF membrane or centrifuge separation.
3. retaining the preparation of the Maca activity essence of natural activity described in a kind of claim 1, it is characterised in that the reservation day
Be added in right active Maca activity essence pharmaceutically acceptable auxiliary material be prepared into powder, tablet, granule, capsule and
Pill.
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| CN103040896A (en) * | 2013-01-06 | 2013-04-17 | 云南圣草峰生物科技有限公司 | Preparation method of maca extractive |
| CN104055183A (en) * | 2014-06-24 | 2014-09-24 | 方国胜 | Health beverage containing Lepidium peruvianum and production method thereof |
| CN104382975A (en) * | 2014-10-24 | 2015-03-04 | 昆明朗盛生物科技有限公司 | Preparation method and application of Maca extractive |
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| CN103040896A (en) * | 2013-01-06 | 2013-04-17 | 云南圣草峰生物科技有限公司 | Preparation method of maca extractive |
| CN104055183A (en) * | 2014-06-24 | 2014-09-24 | 方国胜 | Health beverage containing Lepidium peruvianum and production method thereof |
| CN104382975A (en) * | 2014-10-24 | 2015-03-04 | 昆明朗盛生物科技有限公司 | Preparation method and application of Maca extractive |
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