CN105532686A

CN105532686A - Compound fungicide

Info

Publication number: CN105532686A
Application number: CN201510983382.9A
Authority: CN
Inventors: 杨会营; 王�华; 刘晓娇; 马嵩岳
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Priority date: 2015-12-24
Filing date: 2015-12-24
Publication date: 2016-05-04

Abstract

The invention belongs to the technical field of pesticide, relating to a fungicidal composition. The compound fungicide is characterized by containing two active ingredients of iprovalicarb and phenyl kresoxim-methyl, and is used for preventing and treating cucumber downy mildew, the fungicidal composition can be prepared into a suspending agent, an emulsion in water and a water dispersible granule, has obvious synergistic effect, is long in lasting period and friendly to the environment.

Description

A kind of composite bactericide

Technical field

The invention belongs to technical field of pesticide, contain the composite bactericide of iprovalicarb and ZJ0712 specifically.

Background technology

Iprovalicarb (iprovalicarb) is amino-acid ester analog derivative, has unique brand-new biomimetic features its mechanism of action is different from bactericide that other prevent and treat Oomycete.Its mechanism of action is the synthesis acting on fungal cell wall and protein, can suppress infecting and sprouting of spore, can suppress mycelial growth simultaneously, causes its distortion, death.For Peronosporaceae and the fungus-caused disease of Phytophthora, there is well treatment and eradicant action.For grape, potato, tomato, cucumber, oranges and tangerines, tobacco etc.Prevent and treat downy mildew, epidemic disease etc.Structural formula is:

ZJ0712 (ZJ0712) is the strobilurin fungicide that Zhejiang Provincial Chemical Engineering Research Inst is developed for lead compound with natural antibiotics Strobilurins.ZJ0712 fungicidal spectrum is wide, and bactericidal activity is high, has good protection, treatment, osmosis.Its mechanism of action is by suppressing mitochondrial respiration, destroys the energy synthesis of germ.Therefore, disease is infected in early days there is fabulous protective effect.Bioactivity research result shows: ZJ0712 all has very high bactericidal activity to the plant disease caused by sac fungi, mastigomycetes and imperfect fungus.Excellent control efficiency is all shown to the powdery mildew of various crop, rust, downy mildew, anthracnose, achieves and spray the multiple diseases that a kind of bactericide can control crop mixes generation simultaneously.ZJ0712 is the bactericide of a double treatment of prevention, and after medicament sprays, the photosynthesis of plant strengthens simultaneously, crop can be made to keep verdure in a long time, thus improve the yield and quality of crop.

Cucumber downy mildew is the more serious disease of cucumber, and seedling stage, strain all can be injured, and mainly cause harm blade and stem, tendril and bennet are injured less.Strain is susceptible many after flowering and fruit bearing, nascent water stain shape light green spot on blade, is subject to vein restriction in polygonal yellow green or faint yellow scab, finally becomes brown after expanding.Time serious, scab is merged into large scab, and blade is withered.In wet condition, raw lavender mustiness thing (sporangium and sporangiophore) in the scab back side.If dry, high temperature, mustiness thing disappear or not easily produce.Germ is live body obligatory parasitism fungi, and seed does not carry disease germs, and germ is mainly propagated by air-flow, invades from Stoma of Leaves.Temp. and humidity environmental concerns around the generation of downy mildew and plant is very close, and it is about 16 DEG C that initial temperature occurs, and popular thermophilic is 20 ~ 24 DEG C, and requires that relative moisture is more than 85%.Seedling Stage is fallen ill, and irregular chlorisis yellowish-brown spot occurs in cotyledon front, and strain is fallen ill, and how from forward position, greenhouse, water stain shape, light green fleck appear in morbidity strain before this middle and lower part blade reverse side, and front does not show, and rear scab expands gradually, front exposed.The output of this disease on cucumber has larger impact, market is badly in need of the medicament of the special efficacy to cucumber downy mildew, lasting period length.

Summary of the invention

The object of this invention is to provide and a kind ofly there is synergistic effect and the bactericidal composition of safety and environmental protection, for preventing and treating cucumber downy mildew, compared with existing pesticide control, realizing improving drug effect, decreasing pollution, the object that reduces costs.Be specifically related to the composite bactericide of iprovalicarb and ZJ0712.

Utilizing above-mentioned composite bactericide, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, is suspending agent, aqueous emulsion, water dispersible granules etc.

In above-mentioned composite bactericide, the weight ratio of iprovalicarb and ZJ0712 is 1:60-60:1.

Above-mentioned composite bactericide has following characteristics: the 1. advanced environmental protection of formulation, and environmental pollution is little, does not add organic principle in preparation; 2. bactericidal action is strong, not easily develops immunity to drugs; 3. two kinds of compositions are composite, have synergistic effect.4. the lasting period is long.

Embodiment

For determining iprovalicarb and the composite synergistic effect of ZJ0712 two kinds of active ingredients, first applicant uses these two kinds of compositions to carry out indoor biometrics experiment, experimental technique and result as follows:

First, after analyzing the action character of two kinds of active ingredients, the raw object surveyed is decided to be Cuba artificial downy mildew (Pseudoperonosporacubensis) by us, and this Pseudomonas Mastigomycotina fungi, can cause the downy mildew of cucumber.

The test method of indoor biometrics:

Plate blade method is adopted to measure medicament to the biologically active of bacterium of downy mildew of cucumber.Select cucumber susceptible variety potted plant, from up to down 4 leaf positions, leaf position ~ 6, clip same area, growing way be consistent, with the blade of 1cm ~ 2cm petiole, wrap up petiole with wet cotton balls and be placed in culture dish, moisturizing is for subsequent use.Select incidence of leaf, with 4 DEG C of distillation washing lower blade back side downy mildew sporangiums, (concentration controls every milliliter 1 × 10 to be made into suspension ⁵individual ~ 1 × 10 ⁷individual sporangium), deposit for subsequent use at 4 DEG C.The former medicine of 95% flumorph and the former medicine of 96% ZJ0712 are dissolved with methyl alcohol, with the Tween 80 aqueous solution dilution of 0.05%.According to agent activity, arrange 5 ~ 7 series mass concentration, the final content of organic solvent is no more than 1%.Then liquid is evenly sprayed in vacuum side of blade, after liquid natural air drying, each process blade and blade is gone up dorsad, is emitted in moisture preservation box by after marks for treatment.Test set not containing the process of medicament as blank.Be inoculated in vacuum side of blade, every blade inoculation 4 with ready Fresh spores capsule suspension drop 10 microlitre, often process is no less than 5 leaves.24h inoculation after chemicals treatment, ware lid on inoculation bonnet, is placed in climatic cabinate, and alternately, temperature is 17 DEG C ~ 22 DEG C to the continuous illumination in every day/dark 12h, cultivates under the condition of relative moisture more than 90%.Depending on blank incidence surveying record lesion diameter, and calculate control efficiency.Calculate the EC of each medicament do regression analysis according to the preventive effect probit value of each drug concentration logarithm value and correspondence ₅₀.

The co-toxicity coefficient method representation that the co-toxicity of mixture adopts Sun Yun abundant:

The theoretical toxicity index TTI=∑ of mixture (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).

CTC≤80 are antagonism, and 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect.

Indoor biometrics experimental data sees the following form:

Table 1 iprovalicarb, ZJ0712 and different ratio thereof are to the measurement result of cucumber downy mildew co-toxicity coefficient

Analyze above-mentioned data, obviously can find out that, when iprovalicarb and ZJ0712 are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of cucumber downy mildew is had to the synergistic effect of different proportion.Visible, the composite favourable agents becoming control cucumber downy mildew of iprovalicarb and ZJ0712.

Tested by indoor biometrics, checking iprovalicarb and ZJ0712 two kinds of active ingredients are composite can good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 30.5% iprovalicarb ZJ0712 water dispersible granules

Example two, 31% iprovalicarb ZJ0712 aqueous emulsion

Example three, 22% iprovalicarb ZJ0712 suspending agent

Example four, 18% iprovalicarb ZJ0712 suspending agent

Example five, 10% iprovalicarb ZJ0712 suspending agent

Example six, 12% iprovalicarb ZJ0712 suspending agent

Example seven, 33% iprovalicarb ZJ0712 suspending agent

Example eight, 15.5% iprovalicarb ZJ0712 suspending agent

Example nine, 36.6% iprovalicarb ZJ0712 water dispersible granules

Select above example to carry out preventing and treating the field trial of cucumber downy mildew, determine to fill a prescription and prevent and treat the effect of cucumber downy mildew; Simultaneously by the Contrast on effect with iprovalicarb and ZJ0712 single dose, the control efficiency of the checking embodiment of the present invention.Test site is the Ba Li village, Xianyang, Shanxi province city, and the time is on May 10th, 2013, tests each process four repetition, and community processes the group arrangement of random district, and each plot area is 30m ², adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, and the record dispenser synoptic data of a week is specific as follows:

Table 2 duration of test meteorological data

Investigation method is: every community gets 4 investigation at random, and look into two strains at often, whole blade (in units of blade) is investigated in every strain, and after dispenser, investigation in the 7th day, 14 days, investigates each community disease and refer to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for less than 5% of whole leaf area;

3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;

5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;

7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;

9 grades: lesion area accounts for more than 50% of whole leaf area.

Table 3 iprovalicarb ZJ0712 suspending agent and different agents are to the preventive effect of cucumber downy mildew

As can be seen from above-mentioned data, iprovalicarb and ZJ0712 complex preparation after medicine 7 days preventive effects all more than 70%, and iprovalicarb single dose 7 days preventive effects after medicine are 46.71%, the preventive effect of ZJ0712 single dose is 49.50%, compare iprovalicarb and ZJ0712 single dose, there is the preventive effect of pole significant difference between complex preparation and single dose, after visible two kinds of active ingredients are composite, have good synergistic effect.And after medicine 14 days, all complex preparations have all exceeded 74% to the preventive effect of cucumber downy mildew, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of cucumber downy mildew.Complex preparation can well improve drug effect, extends the lasting period, alleviates the amount of labour of peasant, have very high practicality.

Claims

1. a composite bactericide, is characterized in that: active component is made up of iprovalicarb and ZJ0712, and the weight ratio of described iprovalicarb and ZJ0712 is 1:60 ~ 60:1.

2. composite bactericide according to claim 1, is characterized in that: make suspending agent, aqueous emulsion, water dispersible granules by active component and auxiliary material.