CN105532659A - Method for promoting growth of aquilaria sinensis leaves - Google Patents

Method for promoting growth of aquilaria sinensis leaves Download PDF

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Publication number
CN105532659A
CN105532659A CN201511027437.5A CN201511027437A CN105532659A CN 105532659 A CN105532659 A CN 105532659A CN 201511027437 A CN201511027437 A CN 201511027437A CN 105532659 A CN105532659 A CN 105532659A
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CN
China
Prior art keywords
aquilaria sinensis
leaf
suspension culture
spray
triacontanol
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Pending
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CN201511027437.5A
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Chinese (zh)
Inventor
郑科
谷丽萍
郭永清
陈鹏
张立新
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Yunnan Academy of Forestry
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Yunnan Academy of Forestry
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Priority to CN201511027437.5A priority Critical patent/CN105532659A/en
Publication of CN105532659A publication Critical patent/CN105532659A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18—Nitro compounds
    • A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
    • A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a method for promoting the growth of aquilaria sinensis leaves. According to the method, an aquilaria sinensis leaf growth promoter is applied to aquilaria sinensis at four years old or older on November in the first year and March, April and May in the second year in one cycle, and the promoter is continuously applied to leaf surfaces for 2-3 cycles. The aquilaria sinensis leaf growth promoter is prepared by mixing 2% triacontanol milky powder, 98% compound sodium nitrophenolate technical material and water, wherein the concentration of triacontanol in the aquilaria sinensis leaf growth promoter prepared in a mixed mode is 0.5 ppm, and the concentration of compound sodium nitrophenolate is 12 ppm. By means of the method, before heartwood formation of aquilaria sinensis, the growth of aquilaria sinensis leaves can be effectively promoted, the average number of new leaves growing in each plant is 450, the area of each new leaf is 33 cm<2>, the area of each individual leaf is 70 cm<2> or more, the problem of early-period economic benefits obtained before heartwood formation of aquilaria sinensis at eight years old or older is effectively solved, and effective measures are taken for achieving the purpose of raising aquilaria sinensis with low economic benefits obtained before heartwood formation.

Description

A kind of method promoting suspension culture of Aquilaria sinensis tree leaf growth
Technical field
The invention belongs to Forest Cultivation technical field, be specifically related to the technical method promoting suspension culture of Aquilaria sinensis leaf growth.
Background technology
Suspension culture of Aquilaria sinensis (Aquilariasinensis) is national secondary endangered plant species, and China natural perfume material agalloch eaglewood comes from this seeds." heavy wingceltis dragon musk deer ", agalloch eaglewood is first of many perfume (or spice), and agalloch eaglewood secretes the tissue that grease retains naturally after suspension culture of Aquilaria sinensis seeds trunk suffers damage, agalloch eaglewood is very precious, can be used for Chinese medicine, spices and handicraft, is the active component of 160 kinds of Chinese medicines on market, has temperature to stop the effect received.The natural fragrant storage of current natural world is rare, and artificial Edgeworthia chrysantha technology is still immature, and the suspension culture of Aquilaria sinensis quality obtained, quantity are all very general.But current plantation family among the people is at ground implant mass suspension culture of Aquilaria sinensis such as Yunnan Province of China, Guangxi, Guangdong and Fujian.Due to suspension culture of Aquilaria sinensis tree Edgeworthia chrysantha need a very long time, generally want the diameter of a cross-section of a tree trunk 1.3 meters above the ground to be greater than 15cm, the age of tree will more than 8 ages ability Edgeworthia chrysantha.Before Edgeworthia chrysantha early stage economic benefit solution, how to raise short-long is the key point of industry sustainable development.In recent years, plantation enterprise has carried out the utilization of branch, leaf, such as whitewood spiceleaf tea, suspension culture of Aquilaria sinensis branches and leaves are used for the making of cosmetics, before Edgeworthia chrysantha, suspension culture of Aquilaria sinensis target product is branch, leaf, therefore, promote the growth of suspension culture of Aquilaria sinensis leaf, become the effective measures solving suspension culture of Aquilaria sinensis economic benefit before Edgeworthia chrysantha and raise short-long.
At present, suspension culture of Aquilaria sinensis leaf growth promoting effects foliage fertilizer is executed technology and be there is no precedent both at home and abroad, and this technological invention is pioneering.
Summary of the invention
The object of the invention is to provide and a kind ofly promotes that suspension culture of Aquilaria sinensis sets the method for leaf growth, and for suspension culture of Aquilaria sinensis blade utilizes when not increasing kind of tree planting quantity, furnish ample material supply, increases the income of planting enterprise or self-employed tree cultivator.
Technical scheme of the present invention is:
1. one kind promotes suspension culture of Aquilaria sinensis leaf sheet growth stimulator, by 2% triacontanol milk powder, the former medicine of 98% compound sodium nitrophenolate and water are mixed, mixed by the described promotion suspension culture of Aquilaria sinensis leaf sheet growth stimulator made the concentration of triacontanol be 0.5ppm, the concentration of compound sodium nitrophenolate is 12ppm.
2. promote that suspension culture of Aquilaria sinensis sets a method for leaf growth, comprise the following steps:
(1) spray medicine object: 4 age above suspension culture of Aquilaria sinensis tree;
(2) spraying time: March from First Year November, next year, April and dispenser in May as a samsara, a continuous dispenser 2-3 samsara;
(3) application method: carry out foliage-spray with the promotion suspension culture of Aquilaria sinensis leaf sheet growth stimulator described in technical scheme 1.
The method of the promotion suspension culture of Aquilaria sinensis tree leaf growth 3, according to technical scheme 2, step (3) described foliage-spray is completed before 9 o'clock of every morning, spray repetition more than 3 times at every turn, need guarantee to spray in latter 4 hours at every turn and not rain, as rained, need fill spray, repeat spraying 3 times when at every turn filling spray.
Beneficial effect of the present invention:
Whitewood spiceleaf is at present for leaf tea, and fragrant moxibustion and cosmetics raw material, the enforcement of medicament of the present invention and method, before suspension culture of Aquilaria sinensis Edgeworthia chrysantha, effectively can promote that suspension culture of Aquilaria sinensis sets leaf growth, the 450/strain of average production young leaves, new leaf area 33cm 2/ blade, indivedual new blade area reaches 70cm 2above, achieve unforeseeable technique effect, efficiently solving more than the age of tree 8 age could economical efficiency in early stage before Edgeworthia chrysantha, becomes the effective measures solving suspension culture of Aquilaria sinensis economic benefit before Edgeworthia chrysantha and raise short-long.
Embodiment
In mass fraction, 2% triacontanol milk powder, the former medicine of 98% compound sodium nitrophenolate, 75% gibberellin crystal powder, etc. material be commercially available, the former medicine of 98% compound sodium nitrophenolate containing 5-nitroguaiacol 16.3%, sodium onitrophenol 32.7%, Sodium p-nitrophenoxide 49%.Embodiment is conventional method without specified otherwise.
Embodiment 1 the present invention promotes formula and the preparation method of suspension culture of Aquilaria sinensis leaf sheet growth stimulator
The present invention promotes suspension culture of Aquilaria sinensis leaf sheet growth stimulator formula: be mixed by 2% triacontanol milk powder and the former medicine of 98% compound sodium nitrophenolate and water, and the concentration of triacontanol is 0.5ppm in the promotion suspension culture of Aquilaria sinensis leaf sheet growth stimulator be mixed, the concentration of compound sodium nitrophenolate is 12ppm.
The present invention promotes the preparation method of suspension culture of Aquilaria sinensis leaf sheet growth stimulator: take 2% triacontanol milk powder 0.375g, the former medicine 0.185g of 98% compound sodium nitrophenolate, be watered 15000g namely to make the present invention and promote that suspension culture of Aquilaria sinensis leaf sheet growth stimulator is not (if having trace to take instrument, a large amount of method of dissolving doubling dilution can be adopted, ensure that concentration unanimously).
Embodiment 2
One, experimental scheme
1, take water as solvent, the process of following 9 concentration proportionings that 2% triacontanol milk powder and the former medicine of 98% compound sodium nitrophenolate combine:
Process 1: triacontanol 0.5ppm+ compound sodium nitrophenolate 4ppm.
Process 2: triacontanol 0.5ppm+ compound sodium nitrophenolate 6ppm.
Process 3 (invention formulation): triacontanol 0.5ppm+ compound sodium nitrophenolate 12ppm.
Process 4: triacontanol 0.8ppm+ compound sodium nitrophenolate 4ppm.
Process 5: triacontanol 0.8ppm+ compound sodium nitrophenolate 6ppm.
Process 6: triacontanol 0.8ppm+ compound sodium nitrophenolate 12ppm.
Process 7: triacontanol 1.2ppm+ compound sodium nitrophenolate 4ppm.
Process 8: triacontanol 1.2ppm+ compound sodium nitrophenolate 6ppm.
Process 9: triacontanol 1.2ppm+ compound sodium nitrophenolate 12ppm.
Process 3 is consistent with preparation method described in embodiment 1, the preparation method of all the other 8 process and the present invention described in embodiment 1 promote that the preparation method of suspension culture of Aquilaria sinensis leaf sheet growth stimulator is similar, just concentration is different, weighs medicament grams different, is all dissolved in 15 premium on currency.
2, contrast
Contrast 1 solution: be 75% gibberellin crystal powder 0.5g, first with mass fraction be 95% alcohol dissolve, be then dissolved in 15L water.
Contrast 2 solution: be 75% gibberellin crystal powder 1g, first with mass fraction be 95% alcohol dissolve, be then dissolved in 15L water.
Contrast 3 solution: be 75% gibberellin crystal powder 2g, first with mass fraction be 95% alcohol dissolve, be then dissolved in 15L water.
Contrast 4 solution: for 25g6-benzyladenine is dissolved in 15L water.
Contrast 5 solution: for 37.5g6-benzyladenine is dissolved in 15L water.
Contrast 6: clear water.
Two, method
1, experimental field and seeds: Da Zhai, Jing Ne township, Xishuangbanna Prefecture, Yunnan Province Jinghong City, 4 years raw suspension culture of Aquilaria sinensis tree (Aquilariasinensis), seeding row spacing 2m × 3m.
2, method:
Carry out foliage-spray by above-mentioned each treatment agent and contrast medicament, 9 o'clock of every morning completed and sprayed in the past, sprayed repetition 3 times at every turn, sprayed at every turn and can not rain in 4 hours, as rained, filled spray, repeat spraying 3 times when filling spray.Each process and contrast spray the time above:
First samsara: on November 10th, 2013, on March 20th, 2014, on April 10th, 2014, on May 15th, 2014;
Second samsara: on November 9th, 2014, on March 18th, 2015, on April 12nd, 2015, on May 18th, 2015.
Each treatment agent and each contrast medicament spray 10 strains.Control time is on September 10th, 2014, on September 15th, 2015.
3, observation index: young leaves quantity, young leaves size, young leaves size leaf area is determined, because blade face is oval substantially, calculates with reference to ellipse area, and ellipse long and short shaft value is leaf length and the wide maximum of leaf.
4, interpretation of result
(1) young leaves quantity
Table 12014 year young leaves quantity (spray medicine repeats 10 strains)
Table 22015 year young leaves quantity (spray medicine repeats 10 strains)
By using above-mentioned each reagent treatment, young leaves quantity two annual mean is respectively:
Process 1:343 sheet/strain, process 2:339 sheet/strain, process 3:450 sheet/strain, process 4::350 sheet/strain, process 5:436 sheet/strain, process 6:407 sheet/strain, process 7:373 sheet/strain, process 8:371 sheet/strain, process 9:351 sheet/strain, contrast 1:320 sheet/strain, contrast 2:341 sheet/strain, contrast 3:332 sheet/strain, contrast 4:343 sheet/strain, contrast 5:351 sheet/strain, the average contrasting 6 two years is 268/strain, wherein especially with triacontanol and compound sodium nitrophenolate process 3, process 5, process 6 Be very effective.
According to variance analysis, it is discrepant that various combination uses young leaves sprouting, according to the data of 2015, to interior 10 data of group (i.e. each process, each contrast are to the data of 10 strain tree dispensers), 14 groups carry out analyzing (i.e. 9 process and contrast 1 to contrast 5, due to the clear water process not executing any medicament be starkly lower than 9 process and other contrast, therefore be not counted in analyze)).
p = 1 n ( &Sigma; i = 1 k &Sigma; j = 1 n i x i j ) 2 ; Q = &Sigma; i = 1 k 1 n i ( &Sigma; j = 1 n i x i j ) 2 ; R = &Sigma; i = 1 k &Sigma; j = 1 n i x i j 2
According to formula, result of calculation P=19569178.31, Q=19825699, R=20052850.
S T＝R-p＝483671.7；S A＝Q-P＝256520.7；S E＝R-Q＝227151
Table 3 variance analysis table
(2) young leaves size
Table 42014 year young leaves size average (repeating 10 strains)
Table 52015 year young leaves size average (repeating 10 strains)
After using each medicament, young leaves size two annual mean is respectively:
Process 1:29cm 2/ blade, process 2:33cm 2/ blade, process 3:33cm 2/ blade, process 4:32cm 2/ blade, process 5:29cm 2/ blade, process 6:29cm 2/ blade, process 7:32cm 2/ blade, process 8:32cm 2/ blade, process 9:32cm 2/ blade, contrast 1:25cm 2/ blade, contrast 2:25cm 2/ blade, contrast 3:29cm 2/ blade, contrast 4:25cm 2/ blade, contrast 5:24cm 2/ blade, contrast 6:18cm 2/ blade.
Leaf size there occurs obvious change, and effect is extremely remarkable, and what behave oneself best is the combination of triacontanol and compound sodium nitrophenolate, but combination is inner, and because of the change of concentration, leaf area does not change to some extent.Gibberellin and benzyl aminoadenine aqua take second place, both basic indistinctions.Find in investigation, the process 3 (the present invention) of using triacontanol and compound sodium nitrophenolate and combining, its indivedual new blade area reaches 70cm 2above, average new leaf area 33cm 2/ blade, young leaves 450/strain, effect is extremely remarkable, achieves unforeseeable technique effect.

Claims (3)

1. one kind promotes suspension culture of Aquilaria sinensis leaf sheet growth stimulator, by 2% triacontanol milk powder, the former medicine of 98% compound sodium nitrophenolate and water are mixed, and in the described promotion suspension culture of Aquilaria sinensis leaf sheet growth stimulator be mixed, the concentration of triacontanol is 0.5ppm, and the concentration of compound sodium nitrophenolate is 12ppm.
2. promote that suspension culture of Aquilaria sinensis sets a method for leaf growth, it is characterized in that comprising the following steps:
(1) spray medicine object: 4 age above suspension culture of Aquilaria sinensis tree;
(2) spraying time: March from First Year November, next year, April and dispenser in May as a samsara, a continuous dispenser 2-3 samsara;
(3) application method: carry out foliage-spray with promotion suspension culture of Aquilaria sinensis leaf sheet growth stimulator according to claim 1.
3. the method for promotion suspension culture of Aquilaria sinensis tree leaf growth according to claim 2, it is characterized in that: step (3) described foliage-spray is completed before 9 o'clock of every morning, spray repetition more than 3 times at every turn, need guarantee to spray in latter 4 hours at every turn and not rain, as rained, need fill spray, repeat spraying 3 times when at every turn filling spray.
CN201511027437.5A 2015-12-31 2015-12-31 Method for promoting growth of aquilaria sinensis leaves Pending CN105532659A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1079609A (en) * 1993-03-16 1993-12-22 李俊明 Ginger treasured and production method thereof
CN1317239A (en) * 2001-04-20 2001-10-17 王学虎 Composite plant growth regulator and its preparing process
CN1695454A (en) * 2004-05-13 2005-11-16 青岛科技大学 Nuisance free yield increase element for anti disease and preparation method
CN104692900A (en) * 2013-12-09 2015-06-10 冯祥茂 Walnut foliar fertilizer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1079609A (en) * 1993-03-16 1993-12-22 李俊明 Ginger treasured and production method thereof
CN1317239A (en) * 2001-04-20 2001-10-17 王学虎 Composite plant growth regulator and its preparing process
CN1695454A (en) * 2004-05-13 2005-11-16 青岛科技大学 Nuisance free yield increase element for anti disease and preparation method
CN104692900A (en) * 2013-12-09 2015-06-10 冯祥茂 Walnut foliar fertilizer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张玉臣 等: "不同植物生长调节剂对白木香扦插生根的影响", 《林业科学研究》 *

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