A kind of vitamin D
3soft capsule and preparation method thereof
Technical field
The invention belongs to field of health care food, particularly relate to a kind of vitamin D
3soft capsule and preparation method thereof.
Background technology
Vitamin D
3be a kind of to the mankind and growth of animal, growth, breed, sustain life and ensure healthy indispensable liposoluble vitamin.Its Main Function regulates Ca,P metabolism, promotes absorption and the sclerotin calcification of calcium, phosphorus in field, maintain the balance of blood calcium and serium inorganic phosphorus.Vitamin D
3shortage can cause that rickets, osteomalacia, osteoporosis, thyroid gland technical ability go down etc. disease.Calendar year 2001 Du Xueqin, to the popularity investigation display of the vitamin D deficiency that 1248, Chinese Beijing area, 12 ~ 14 years old Pubertal girls carries out, has the people of 45.2% to be in the clinical deficiency state of vitamin D in the winter that sunshine is more weak.The ability of skin synthesis of vitamin d precursor declines gradually along with the stingy of age on the other hand, adds that the elderly decreases outdoor activity because mobility is limited, can infer that the shortage of vitamin D is generally deposited in healthy elderly.As can be seen here, vitamin d insufficiency is still common Problems existing.
Chinese patent application 201010032463.8 provides a kind of dimension D calcium soft capsule and preparation method thereof, and comprising: Paris white, vitamine D3, soybean salad oil, soybean lecithin, beeswax, this dimension D calcium soft capsule can supplement a certain amount of vitamin D
3and calcium, but the vitamin D of this dimension D calcium soft capsule
3content is few, poor stability, easy photodissociation, and its nutritional labeling is more single.
Therefore, be necessary to provide a kind of and have good stability, the vitamin D of not oxidizable, nutritional labeling diversification
3soft capsule.
Summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides a kind of nutritional labeling diversification, for human body supplement enough vitamin Ds while promote the absorption of calcium phosphorus, this vitamin D
3soft capsule has good stability, not oxidizable and photodissociation.Inventor, by the checking repeatedly of clinical practice, adjusts amount and the kind of each component, has finally invented the vitamin D of a nutritional labeling diversification
3soft capsule.
To achieve these goals, technical scheme of the present invention is as follows:
A kind of vitamin D
3soft capsule, comprises content and softgel shell, and described content comprises the component of following parts by weight: vitamin D
310 ~ 30 parts, vitamin D
21 ~ 6 part, walnut oil 3 ~ 10 parts, eucommia ulmoides seed oil 2 ~ 6 parts, granada seed oil 2 ~ 6 parts, 6 ~ 15 parts, amino acid, calcium citrate 0.5 ~ 3 part, antioxidant 3 ~ 6 parts and stabilizing agent 0.2 ~ 3 part; Described softgel shell comprises the component of following parts by weight: 25 ~ 50 parts, gelatin, glycerine 15 ~ 20 parts, titanium dioxide 2 ~ 3 parts and purified water 10 ~ 20 parts.
Preferably, described content comprises the component of following parts by weight: vitamin D
310 parts, vitamin D
21 part, walnut oil 3 parts, eucommia ulmoides seed oil 2 parts, granada seed oil 2 parts, 6 parts, amino acid, calcium citrate 1 part, antioxidant 3 parts and stabilizing agent 1 part.
Preferably, described content comprises the component of following parts by weight: vitamin D
320 parts, vitamin D
24 parts, walnut oil 6 parts, eucommia ulmoides seed oil 4 parts, granada seed oil 4 parts, 9 parts, amino acid, calcium citrate 2 parts, antioxidant 4.5 parts and stabilizing agent 2 parts.
Preferably, described content comprises the component of following parts by weight: vitamin D
330 parts, vitamin D
26 parts, walnut oil 10 parts, eucommia ulmoides seed oil 6 parts, granada seed oil 6 parts, amino acid/11 2 parts, calcium citrate 3 parts, antioxidant 6 parts and stabilizing agent 3 parts.
Preferably, described antioxidant is made up of by the weight ratio of 2 ~ 4:1 ~ 2 vitamin C and vitamin E.
Preferably, described stabilizing agent is selected from beeswax, suet and soybean lecithin.
Preferably, described amino acid is at least one in lysine, threonine, glutamic acid and isoleucine.
Preferably, described softgel shell also comprises iron oxide 1 ~ 3 part and anticorrisive agent 0.1 ~ 1 part.
Correspondingly, present invention also offers described vitamin D
3the preparation method of soft capsule, it comprises the following steps:
S1: take purified water and glycerine mixing and stirring in stainless-steel pan respectively, add described gelatin, temperature controls decompression at 30 ~ 40 DEG C and stirs, and is warming up to 50 ~ 80 DEG C, stirs and above-mentioned gelatin particle is dissolved completely after described gelatin expands; Add described titanium dioxide, decompression stirring makes it to dissolve completely evenly, filters, and filtrate 50 ~ 60 DEG C insulation leaves standstill, and obtains softgel shell glue, stand-by;
S2: take described stabilizing agent, antioxidant, vitamin D respectively
3and vitamin D
2, mixing and stirring; Add described walnut oil, eucommia ulmoides seed oil, granada seed oil, amino acid and calcium citrate, stir, cross colloid mill homogeneous 3 ~ 5 times, each homogeneous 15 ~ 30min, the i.e. suspension of obtained appearance uniform, is placed in material-compound tank and stirs 60 ~ 100min by suspension, i.e. obtained described content;
S3: control temperature is at 20 ~ 25 DEG C, by above-mentioned softgel shell glue and content automatic rotation pellet processing machine pill, it is 0.8 ~ 1.0mm that shell thickness controls, and washes away capsule and pill surface oil stain with ethanol, at 30 ~ 35 DEG C, drying 10 ~ 20 hours, packs and obtains described vitamine D3 soft capsule.
Preferably, in described S1 step, anticorrisive agent and iron oxide is added.
Vitamin D described in the present invention
3the absorption of human body to calcium, phosphorus can be improved, the level of plasma calcium and blood plasma phosphorus is reached capacity degree, growth promoting effects and bone calcification, promote that tooth perfects; Increased the absorption of phosphorus by intestines wall, and increase the absorption of phosphorus by renal tubule; Maintain the normal level of citrate in blood: prevent amino acid from being lost by kidney.For the control of rickets, malacosteon and infantile tetany.
Described walnut oil is rich in the multiple unsaturated acids up to about 90%, wherein linoleic acid content is more, for 3 to 4 times of common rapeseed oil content, especially eat with the crowd of applicable pregnant woman, infant, student, white collar and overwork of requiring mental skill, be called it is " melatonin " in food by numerous personages.
In described eucommia ulmoides seed oil, unsaturated fatty acid content is 91.18%, and aliphatic acid consists of leukotrienes 67.38%, linoleic acid 9.97%, oleic acid 15.81%, stearic acid 2.15%.Palmitic acid 4.68%, leukotrienes is main in vivo to be existed with the form of DHA and EPA.There is hypotensive, reducing blood lipid, raising memory and vision, promote that infant brain is grown, antiallergy, the function such as anticancer, antitumor, all have positive effect to enhancing memory function, analgesia, antifatigue, anti-ageing, antitumor, immunity moderation function etc., especially unique bidirectional modulation immunologic function is to safeguarding that the health of human body plays vital effect.
Described granada seed oil is the how unsaturated conjugated aliphatic acid of unique plant source, and its main component is punicic acid, and content can reach 70-80%.Because of the structure of punicic acid and CLA (CLA) the most close to and be referred to as " super CLA ", its functional CLA exceeded in general sense.Granada seed oil is also rich in Antioxidative Factors; the ability that there is very strong removing human free radical and delay senility; granada seed oil can protect human immune system; prevention of arterial is atherosis; reduce canceration, help human body opposing cancer, obesity, diabetes and heart disease, the ability that there is very strong removing human free radical He delay senility; also contain the plant female hormone similar to the female hormone structure that human body natural produces in granada seed oil simultaneously, contribute to alleviating women's climacteric syndrome.
Described calcium citrate is compared with calcium carbonate, calcium citrate does not need hydrochloric acid in gastric juice activated absorption, absorptivity is good, gaseous distention can not be caused in vivo as calcium carbonate produces carbon dioxide, harmless to alimentary canal mucous membrane, calcium citrate is to the even stronger complexing of calcium simultaneously, when the concentration of calcium citrate in vivo increases, it can in conjunction with free calcium ion, and the calcium of replacing in such as calcium oxalate, calcium phosphate, form stable complex compound soluble in water, discharge in body, inhibit calcium salt (calcium oxalate etc.) due to over-saturation state the lithogenous process of crystallize out shape.
Compared with prior art, the present invention has following technical advantage:
1, vitamin D provided by the invention
3soft capsule contains multivitamin and various plants oil, promote the absorption of calcium phosphorus while for human body vitamin D, and multiple nutritional components can be provided for human body, have and strengthen cerebral function, enhancing immune function of human body, promote brain and nervous system development, remove interior free yl, prevent the effects such as multiple chronic disease generation.
2, vitamin D provided by the invention
3soft capsule, as the outer used time, can also play whitening, moisturizing, crease-resistant effect.
3, vitamin D provided by the invention
3stability of Soft Capsules is good, has not easily photodissociation, not oxidizable advantage.
4, vitamin D of the present invention
3soft capsule preparation technology is simple, is convenient to industrial mass production.
Detailed description of the invention
It will be understood by those skilled in the art that technology disclosed in following examples represents the technology playing good action in the practice of the invention of the present inventor's discovery.But, many changes can be made in disclosed specific embodiments, and still obtain same or analogous result, and not depart from the spirit and scope of the present invention.
Vitamin D described in the embodiment of the present invention
3in soft capsule, the information such as producer and article No. of constituent part is as follows:
Embodiment 1: vitamin D
3soft capsule.
The vitamin D of the embodiment of the present invention 1
3soft capsule comprises content and softgel shell, and described content comprises the component of following parts by weight: vitamin D
310 parts, vitamin D
21 part, walnut oil 3 parts, eucommia ulmoides seed oil 2 parts, granada seed oil 2 parts, 6 parts, amino acid, calcium citrate 1 part, antioxidant 3 parts and stabilizing agent 1 part; Described softgel shell comprises the component of following parts by weight: 25 parts, gelatin, glycerine 15 parts, titanium dioxide 2 parts and purified water 10 parts.
Wherein, described antioxidant is made up of Catergen part and vitamin e1 part, and described stabilizing agent is made up of 0.2 part, beeswax, suet 0.3 part and soybean lecithin 0.5 part.
Preparation method:
S1: get purified water and glycerine mixing and stirring in stainless-steel pan, add gelatin, temperature controls decompression at 30 DEG C and stirs, and is warming up to 60 DEG C, stirs and gelatin particle is dissolved completely after described gelatin expands; Add titanium dioxide, decompression stirring makes it to dissolve completely evenly, filters, and filtrate 50 DEG C insulation leaves standstill, and obtains softgel shell glue, stand-by;
S2: get beeswax, suet, soybean lecithin, vitamin C, vitamin E, vitamin D
3and vitamin D
2, mixing and stirring; Add described walnut oil, eucommia ulmoides seed oil, granada seed oil, amino acid and calcium citrate, stir, cross colloid mill homogeneous 3 times, each homogeneous 20min, namely the suspension of obtained appearance uniform, is placed in material-compound tank by suspension and stirs 60min, i.e. obtained described content;
S3: control temperature is at 20 DEG C, and by above-mentioned softgel shell glue and content automatic rotation pellet processing machine pill, shell thickness controls as 0.8mm, and wash away capsule and pill surface oil stain with ethanol, drying 15 hours, packs and obtain described vitamin D at 35 DEG C
3soft capsule.
Embodiment 2: vitamin D
3soft capsule.
The vitamin D of the embodiment of the present invention 2
3soft capsule comprises content and softgel shell, and described content comprises the component of following parts by weight: vitamin D
320 parts, vitamin D
24 parts, walnut oil 6 parts, eucommia ulmoides seed oil 4 parts, granada seed oil 4 parts, 9 parts, amino acid, calcium citrate 2 parts, antioxidant 4.5 parts and stabilizing agent 2 parts; Described softgel shell comprises the component of following parts by weight: 35 parts, gelatin, glycerine 15 parts, titanium dioxide 2 parts, iron oxide 2 parts, anticorrisive agent 0.5 part and purified water 15 parts.
Wherein, described antioxidant is made up of vitamin C 3 parts and vitamin e1 .5 part, and described stabilizing agent is made up of 0.5 part, beeswax, suet 0.5 part and soybean lecithin 1 part.
Preparation method:
S1: get above-mentioned purified water, glycerine, anticorrisive agent mixing and stirring in stainless-steel pan, add above-mentioned gelatin, temperature controls decompression at 36 DEG C and stirs, and is warming up to 60 DEG C, stirs and gelatin particle is dissolved completely after described gelatin expands; Add titanium dioxide and iron oxide, decompression stirring makes it to dissolve completely evenly, filters, and filtrate 55 DEG C insulation leaves standstill, and obtains softgel shell glue, stand-by;
S2: get above-mentioned beeswax, suet, soybean lecithin, vitamin C, vitamin E, vitamin D
3and vitamin D
2, mixing, stirs; Add described walnut oil, eucommia ulmoides seed oil, granada seed oil, amino acid and calcium citrate, stir, cross colloid mill homogeneous 4 times, each homogeneous 20min, namely the suspension of obtained appearance uniform, is then placed in material-compound tank by suspension and stirs 80min, i.e. obtained described content;
S3: control temperature is at 22 DEG C, and by above-mentioned softgel shell glue and content automatic rotation pellet processing machine pill, shell thickness controls as 0.9mm, washes away capsule and pill surface oil stain with ethanol, and drying 15 hours at 35 DEG C, packaging makes the vitamin D of 300mg/ grain
3soft capsule.
Embodiment 3: vitamin D
3soft capsule.
The vitamin D of the embodiment of the present invention 3
3soft capsule comprises content and softgel shell, and described content comprises the component of following parts by weight: vitamin D
330 parts, vitamin D
26 parts, walnut oil 10 parts, eucommia ulmoides seed oil 6 parts, granada seed oil 6 parts, amino acid/11 2 parts, calcium citrate 3 parts, antioxidant 6 parts and stabilizing agent 3 parts; Described softgel shell comprises the component of following parts by weight: 50 parts, gelatin, glycerine 20 parts, titanium dioxide 3 parts, iron oxide 3 parts, anticorrisive agent 1 part and purified water 20 parts.
Wherein, described antioxidant is made up of vitamin C 4 parts and vitamin E2 part, and described stabilizing agent is made up of 0.5 part, beeswax, suet 0.5 part and soybean lecithin 2 parts.
Preparation method:
S1: get above-mentioned purified water, glycerine, anticorrisive agent mixing and stirring in stainless-steel pan, add above-mentioned gelatin, temperature controls decompression at 40 DEG C and stirs, and is warming up to 80 DEG C, stirs and gelatin particle is dissolved completely after described gelatin expands; Add titanium dioxide and iron oxide, decompression stirring makes it to dissolve completely evenly, filters, and filtrate 60 DEG C insulation leaves standstill, and obtains softgel shell glue, stand-by;
S2: get above-mentioned beeswax, suet, soybean lecithin, vitamin C, vitamin E, vitamin D
3and vitamin D
2, mixing, stirs; Add described walnut oil, eucommia ulmoides seed oil, granada seed oil, amino acid and calcium citrate, stir, cross colloid mill homogeneous 5 times, each homogeneous 30min, namely the suspension of obtained appearance uniform, is then placed in material-compound tank by suspension and stirs 100min, i.e. obtained described content;
S3: control temperature is at 24 DEG C, and by above-mentioned softgel shell glue and content automatic rotation pellet processing machine pill, shell thickness controls as 1.0mm, washes away capsule and pill surface oil stain with ethanol, and drying 20 hours at 35 DEG C, packaging makes the vitamin D of 300mg/ grain
3soft capsule.
Comparative example 1: vitamin D
3soft capsule.
Described antioxidant is made up of vitamin C 1 part and vitamin E 3 parts, and all the other compositions are as embodiment 2.
Preparation method's reference example 2.
Comparative example 2: vitamin D
3soft capsule.
Described stabilizing agent is made up of 0.5 part, beeswax and soybean lecithin 1.5 parts, and all the other compositions are as embodiment 2.
Preparation method's reference example 2.
Stability test
1, trial drug and grouping
Embodiment 1 group: the embodiment of the present invention 1 vitamin D
3soft capsule;
Embodiment 2 groups: the embodiment of the present invention 2 vitamin D
3soft capsule;
Embodiment 3 groups: the embodiment of the present invention 3 vitamin D
3soft capsule;
Comparative example 1 group: comparative example 1 vitamin D of the present invention
3soft capsule;
Comparative example 2 groups: comparative example 2 vitamin D of the present invention
3soft capsule;
Control group: gummy shark vitamin D soft capsule.
2, test method
After above-described embodiment 1 ~ 3 group, comparative example 1 ~ 2 group and control group trial drug are placed 200 hours under the fluorescent lamp of 3000 luxs, use high performance liquid chromatograph, under following condition determination, obtain vitamin D in each group
3survival rate, utilize the postradiation soft capsule of visual relatively light with without the tone of light-struck soft capsule wafer, evaluate the stability of tone.
Survival rate condition determination
Device: Shimadzu Seisakusho Ltd. high performance liquid chromatograph (Autoinjector-SIL-10A, liquor charging unit LC-10AD, system controller SCL-10A, detector SPD-10A, post baking oven (columnoven) CTD-10A/10AC, ChromatopackC-R7Apuls); Post: Waters SymmetryC183.5um4.6 × 150mm; Sample size: 200 microlitres (sample solution concentration is about 125ng/ml); Column temperature: 25 DEG C; Mobile phase: acetonitrile: water: oxolane: acetic acid=1350:400:250:1(V/V); Flow velocity: 1ml/min; Detector: UV detector 265nm.
3, measurement result is as shown in table 1.
Table 1 survival rate measurement result
Group | Vitamin D
3Survival rate (%)
|
Control group | 74.2 |
Embodiment 1 group | 96.2 |
Embodiment 2 groups | 98.1 |
Embodiment 3 groups | 95.5 |
Comparative example 1 group | 75.5 |
Comparative example 2 groups | 70.1 |
4, conclusion.
As seen from the above table, the present embodiment 1 ~ 3 group of vitamin D
3the fluorescent lamp of soft capsule through 3000 luxs be after 200 hours, its survival rate all more than 90%, all far away higher than control group, wherein, with embodiment 2 groups of vitamin Ds
3the survival rate of soft capsule is the highest, reaches 98.1%, therefore embodiment 2 groups is preferred embodiment; Comparative example 1 group changes the amount ratio of vitamin C and vitamin E in antioxidant, and its survival rate is only 83.5%, is starkly lower than embodiment 1 ~ 3 group, therefore can learns, the amount comparison vitamin D of the present invention of vitamin C and vitamin E
3there is impact in the antioxygenic property of soft capsule; Comparative example 2 groups changes the kind composition of stabilizing agent, and its survival rate is also starkly lower than embodiment 1 ~ 3 group, as can be seen here, and vitamin D of the present invention
3the kind of soft capsule vitamin C and vitamin E, stabilizing agent is through lot of experiments and screens the optimised quantity ratio and kind that draw, therefore, and vitamin D of the present invention
3soft capsule has good stability, and it is photodissociation and oxidation not easily, and retention cycle is long.
In following test, because the stability of comparative example 1,2 groups is not good, therefore not it can be used as test target.
Test example one vitamin D of the present invention
3soft capsule is on the impact of mouse brain function
1, experimental animal and grouping
Choose healthy mice 50, male and female half and half, 19 ~ 25g, be divided into 5 groups at random, often organize 10, be respectively vitamin D of the present invention
3the high, medium and low dosage group of soft capsule, control group and model group.
2, medication
Embodiment 2 vitamin D
3soft capsule high dose group, gavage gives 45mg/kg; Every day gavage 1 time, continuous gavage 15 days;
Embodiment 2 vitamin D
3dosage group in soft capsule, gavage gives 22.5mg/kg; Every day gavage 1 time, continuous gavage 15 days;
Embodiment 2 vitamin D
3soft capsule low dose group, gavage gives 11.5mg/kg; , every day gavage 1 time, continuous gavage 15 days;
Control group: Kang man of great strength's linseed oil soft capsule, gavage gives 75mg/kg; Every day gavage 1 time, continuous gavage 15 days;
Model group: gavage gives isopyknic physiological saline (10ml.kg
-1); Every day gavage 1 time, continuous gavage 15 days.
3, test method
From administration the 16th day, after each administration 2 hours, mouse is put into reaction chamber endoadaptation environment 3 minutes, then be energized immediately, mouse is subject to galvanism, its normal reaction be rebound platform to hide noxious stimulation, each training 5 minutes, and record the number of times that every mouse shocked by electricity, in this, as school grade.Repeat above-mentioned test after 24 hours, namely this remember and keep test, jumps off the number of times (mistake sum) of irriate in incubation period and 3 minutes that record first time jumps off platform.
4, statistical analysis
Each group of test data adopts the process of SPSS17.0 software statistics, and compare employing variance analysis between many groups, P < 0.05 represents that difference has statistically significant meaning.
5, result of the test is as shown in table 2.
Table 2 test result (± S, n=10)
Note:
▲compared with model group, p<0.05;
▲ ▲compared with model group, p<0.01; * compared with control group, p<0.05.
6, conclusion
As shown in Table 2, successive administration is after 15 days, control group and the embodiment of the present invention 2 vitamin D
3the high, medium and low dosage group of soft capsule all can improve the learning and memory ability of mouse significantly, and has significant difference compared with model group; Wherein with the embodiment of the present invention 2 vitamin D
3the learning and memory ability of soft capsule high dose group mouse is best; Control group is Kang man of great strength's linseed oil soft capsule, and its main component comprises linseed oil, fish oil and wheat-germ oil, and it has raising cerebral function, strengthens brain activity, the effects such as develop immunitypty, the embodiment of the present invention 2 vitamin D
3soft capsule high dose group, compared with control group, all has significant difference p<0.05 in learning ability of mice and memory capability; As can be seen here, vitamin D of the present invention
3the effect of soft capsule in enhancing cerebral function is better than Kang man of great strength's linseed oil soft capsule, i.e. vitamin D of the present invention
3soft capsule has the effect strengthening cerebral function significantly, improve memory.
Test two vitamin Ds of the present invention
3soft capsule is on the impact of immune function of mice
1, experimental animal and grouping
Choose healthy mice 40, male and female half and half, 19 ~ 25g, be divided into 4 groups at random, often organize 10, be respectively vitamin D of the present invention
3the high, medium and low dosage group of soft capsule and control group.
2, medication
Embodiment 2 vitamin D
3soft capsule high dose group, gavage gives 45mg/kg; Every day gavage 1 time, continuous gavage 15 days;
Embodiment 2 vitamin D
3dosage group in soft capsule, gavage gives 22.5mg/kg; Every day gavage 1 time, continuous gavage 15 days;
Embodiment 2 vitamin D
3soft capsule low dose group, gavage gives 11.5mg/kg; , every day gavage 1 time, continuous gavage 15 days;
Model group: gavage gives isopyknic physiological saline (10ml.kg
-1); Every day gavage 1 time, continuous gavage 15 days.
3, test method
From administration the 16th day, after administration, 6 hours post-tensioning necks put to death mouse, take the weight (representing by organ weight/10g body weight) often organizing mouse thymus and spleen respectively.
4, statistical analysis
Each group of test data adopts the process of SPSS17.0 software statistics, and compare employing variance analysis between many groups, P < 0.05 represents that difference has statistically significant meaning.
5, result of the test is as shown in table 3.
Table 3 embodiment of the present invention 2 vitamin D
3soft capsule is on the impact (± S, n=10) of immune function of mice
Group | N(only) | Dosage (mg/kg) | Thymic weight (mg/10g body weight) | Spleen gland weight (mg/10g body weight) |
Model group | 10 | - | 27.52±10.36 | 57.34±22.65 |
High dose group | 10 | 45 | 35.28±14.07
▲▲ | 63.12±25.09
▲▲ |
Middle dosage group | 10 | 22.5 | 32.17±12.51
▲▲ | 61.29±24.39
▲▲ |
Low dose group | 10 | 11.5 | 28.39±11.35 | 58.30±23.18 |
Note:
▲compared with model group, p<0.05;
▲ ▲compared with model group, p<0.01.
6, conclusion.
As shown in Table 3, successive administration is after 15 days, compared with model group, and the embodiment of the present invention 2 vitamin D
3high, middle group of soft capsule can improve the weight of immune organ significantly, and has significant difference compared with model group, wherein best with the effect of high dose group, with as can be seen here, and the embodiment of the present invention 2 vitamin D
3soft capsule has the effect significantly strengthening immune function of human body.
Owing to describing the present invention by above preferred embodiment, in spirit of the present invention and/or scope, any for replacement/of the present invention or combination implement the present invention, be all apparent for a person skilled in the art, and be included among the present invention.