CN105476014A - Method for preparing biological zinc from oysters - Google Patents
Method for preparing biological zinc from oysters Download PDFInfo
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- CN105476014A CN105476014A CN201511018396.3A CN201511018396A CN105476014A CN 105476014 A CN105476014 A CN 105476014A CN 201511018396 A CN201511018396 A CN 201511018396A CN 105476014 A CN105476014 A CN 105476014A
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Abstract
The invention discloses a method for preparing biological zinc from oysters. The method comprises the following steps: removing shells of living oysters, grinding fresh oyster meat by a colloid mill to obtain meat pulp, adding cellulase, fungal proteinase and lipase for enzymolysis, performing fine filtering, separating to obtain filter liquor rich in biological zinc, separating the filter liquor with a molecular sieve, performing vacuum concentration, and performing spray drying to obtain 100-150-mesh biological zinc dry powder. The biological zinc drying powder has the characteristics of being high in water solubility, uniform in molecular weight, high in biological activity, free from activation, direct and fast in absorption, free of fishy smell of the oysters, and the like, and is a good functional factor in healthy products having high additional values.
Description
Technical field
The present invention relates to one utilizes oyster to prepare biological zinc method, is specifically prepared the method for biological zinc by enzymolysis, molecular sieve separation, concentration, spraying dry with fresh and alive oyster.
Background technology
Oyster (Oyster), is commonly called as oyster, oyster, oyster is yellow, and be first cultivated shellfish in the world, Ye Shi China important marine shellfish, belong to Ostreidae, bivalve, is distributed in each ocean in temperate zone and the torrid zone stretch of coastal water.Oyster meat is nutritious, have the laudatory title of " seabed milk ", and west is called " god grant evil spirit food ", Japan's reputation be " extra large profound rice, the source of root ".According to scientific research analysis, the protein content in dry oyster meat can reach 45-47w/w%, and according to the World Food Programme's evaluation, in oyster meat, essential amino acid mass ratio and completeness are better than human milk and cow's milk wherein oyster amount of iodine is higher than cow's milk 200 times.Containing abundant glycogen in oyster, account for the 20-40w/w% of dry weight.In oyster, fat content (dry weight) is 7-11w/w%, mostly be the polyunsaturated fatty acid with physiologically active, as: phosphoinositide, composite phospholipid, DHA DHA, eicosapentaenoic acid EPA etc., these active matters have good curative effect on treatment cardiovascular and cerebrovascular disease.In addition; also containing vitamin A, B, C, D, E; taurine and trace element are particularly rich in zinc, selenium and other trace elements; the content of biological zinc is that in current human foods, content is the highest; just containing zinc 47mg in 100 grams of oyster meats; for equivalent weight thin pork 30 times, 76 times of sea cucumber, 500 times of apple.
Oyster not only has very high nutritive value, also has important medical value.Its biologically active and medical care effect unique, wherein some biologically active and drug effect are that general tonic is incomparable.In China's medicine historical book treatise on Febrile Diseases, Thousand Golden Prescriptions, Compendium of Material Medica, the medical value of oyster is all on the books.On the Pharmacopoeia of the People's Republic of China, then record oyster " softening and resolving hard mass, restrains astringent or styptic treatment for spontaneous sweating for tranquilizing and allaying excitement, nourishment for vitality, and for insomnia of palpitating with fear, dizzy tinnitus, the sick productive cough of scrofula, certainly, spontaneous sweating, seminal emission collapses band, hydrochloric acid in gastric juice pantothenic acid to disease addiction ruffian ".In view of abundant nutrition and the function curative effect of oyster, oyster is classified as first and is food and the food of medicinal material by the Ministry of Public Health of China approved.
Zinc is one of 59 kinds of trace elements of needed by human, is about 2g ~ 3g at adult body intensive amount, and be present in all tissues, liver, kidney, pancreas, brain etc. organize zinc content more.Zn content needed for human body every day is 15mg, takes in mainly through ordinary meal.Zinc has very important physiological function, with more than 300 kind of enzymatic activity or relevant with enzymatic structure in body, to promoting acid-base balance in body development and bone growth, regeneration, adjustment cell, promote the normal formation of sense of taste sense of smell, the eubolism of promotion, maintain skin health, strengthen immunity function, prevent the aspect such as aging from having very important significance.
Biological zinc also claims zinc albuminate, zinc albumen, zinc-binding protein, is the bond of protein and zinc, the compound form that the connected mode between them combines with covalent bond, or the chelate based on coordinate bond, is the bion zinc source that the third generation supplements zinc product.For obtaining biological zinc, scientific researcher makes by adding in organic zinc or inorganic zinc to culture medium the high Zn-contained yeast that biological zinc is rich in the acquisition of nutrients cultivation microbial method, obtains bird height zinc egg etc. by feeding containing zinc feed.By biological zinc, there is important physiologically active, oyster itself has high level natural biological zinc simultaneously, how complete for natural production zinc and keep its physiologically active, effectively extract, make the product played an important role to human health, a lot of scholar of recent domestic conducts in-depth research and gets certain effect.One as open in Chinese patent CN201010234452.8 synchronously extracts taurine from oyster, the method of biological zinc and polypeptide, pass through ultrasonic disruption, poach, purifying resin, concentrated grade obtains biological zinc preparation method disclosed in biological zinc and " food development and research " scientific paper the extraction preparation method research of biological zinc " in the Crassostrea rivularis " (volume the 14th phase July the 36th in 2015) and prepares biological zinc method by ultrasonic disruption, it is owing to producing bubble when ultrasonic fragmentation is and utilizes high frequency mechanical wave to act on solution that the method exists shortcoming, there is cavitation phenomenon in bubble parameters and fragmentation, and the shock wave that cavitation phenomenon causes and shearing force make cell rupture.Ultrasonic wave itself causes shock wave and shearing force is non-directional and molecular cleavage ability, therefore free biological zinc can only be discharged, cannot discharge with the biological zinc of zinc protein structure for major part, and free biological zinc molecular weight differs, cause bio-absorbable utilization rate low, absorb the shortcomings such as slow.
Summary of the invention
The object of the present invention is to provide one to utilize oyster to prepare biological zinc method, this biological zinc has water-quick-soluble, molecular weight uniform, high bioactivity, do not need activation, directly to absorb and fast, without features such as oyster fishy smell, is high added value health-oriented products.
The present invention is achieved through the following technical solutions above-mentioned purpose: one utilizes oyster to prepare biological zinc method, comprises the steps:
(1) oyster that lives is shelled through cleaning, and obtains fresh oyster fresh meat;
(2) doubly add the purified water of 3-5 DEG C by the 1-2 of fresh oyster fresh meat weight, add 0.05-0.15w/w%, vigor is the lysozyme of 1.5-1.8U/g source egg white, wears into fineness at 0.15-0.25mm slurry with colloid mill;
(3) slurry of step (2) gained is placed in bioreactor, add cellulase that 0.3-0.5w/w% vigor is 20-25 ten thousand U/g, 1.5-2.5w/w% vigor be 3-4 ten thousand U/g fungal proteolytic enzyme and 0.5-0.8w/w% vigor is 8-10U/g lipase, at temperature 35-42 DEG C, speed of agitator 150r/min conditioned response 10-20 hour, obtains enzymolysis liquid;
(4) by step (3) gained enzymolysis liquid through centrifugation 15min, isolate precipitum and centrifugate, precipitum for the preparation of oyster nutrition powder, centrifugate accurate filter filter obtain filtrate;
(5) obtaining molecular weight uniform concentration after step (4) gained filtrate being separated by molecular sieve separation equipment is that 15-20 ° of BX is rich in oyster biological zinc solution;
(6) be that 15-20 ° of BX is rich in oyster biological zinc solution at temperature 50-65 DEG C, Vacuum Concentration under vacuum absolute value 0.08-0.09 condition by step (5) concentration, obtain concentration be 35-40 ° of BX be rich in oyster biological zinc solution;
(7) be that 35-40 ° of BX is rich in oyster biological zinc solution and obtains 100-150 object oyster biological zinc dry powder by spray drying device by step (6) concentration.
The described colloid mill rotating speed of step (2) is 1500-2200r/min.
Centrifugation described in step (4) is 800mm at centrifugal radius, and rotating speed is carry out under the condition of 2500-3500r/min.
The splitter blade diameter length ratio of step (5) described molecular sieve equipment is 1:20-40, and linear flow speed is 10-25L/min, and molecular sieve particle diameter is 0.15-0.2mm, and molecular screen material is chromatographic isolation front resin transition.
Step (7) described spray drying device is atomizer drier, and EAT is 135-145 DEG C, air outlet temperature 95-105 DEG C.
Unless otherwise indicated, percentage of the present invention is mass percent, and each constituent content percentage sum is 100%.
Outstanding advantages of the present invention is:
1, adding bio-preservative lysozyme in oyster fresh meat prevents oyster meat Middle nutrition active matter, biological zinc, glycogen from being resolved into invalid thing by other microorganism or enzyme;
2, add cellulase, lipase complex enzyme makes nutrition discharge more completely more thoroughly muscle fibre, lipolysis in oyster meat, make the more efficient enzymolysis of Fungal Protein hydrolase become more Small molecular biological zinc protein molecular to be more suitable for human body in conjunction with zinc protein or complexing zinc albumen and directly inhale biological zinc molecule.
3, utilize molecular sieve to be separated, ensure that gained biological zinc molecular weight is homogeneous and be applicable to, ensure biological zinc high-performance bio absorptivity.
Detailed description of the invention
By the following examples technical scheme of the present invention is described further.
Embodiment 1
The example utilizing oyster to prepare biological zinc method of the present invention, comprises the steps:
The oyster that lived by 35kg is shelled through cleaning, add the purified water of 2 times 3 DEG C, adding 0.05w/w% vigor is the lysozyme of 1.5 ten thousand/g from egg white, the colloid mill being 1500r/min at rotating speed wears into fineness at 0.15mm, be placed in bioreactor, adding 0.3w/w% vigor is 200,000 U/g cellulases, 1.5w/w% vigor is 30,000 U/g fungal proteolytic enzyme and 0.5w/w% vigor is 80,000 U/g lipase, temperature 35 DEG C, rotating speed 150r/min conditioned response was 800mm at centrifugal radius after 10 hours, rotating speed is 2500r/min centrifugation 15min, precipitum is for the preparation of oyster nutrition powder, centrifugate accurate filter filters, filtrate is 1:20 by blade diameter length ratio, linear flow speed is 10L/min, molecular sieve filled material is chromatographic isolation front resin transition, particle diameter is that after the molecular sieve of 0.15mm is separated to obtain molecular weight uniform concentration be that 15 ° of BX are rich in oyster biological zinc solution, at temperature 50 C, under vacuum absolute value 0.08 condition, Vacuum Concentration obtains the oyster biological zinc solution that is rich in of 35 ° of BX is 135 DEG C by EAT, and the atomizer drier of air outlet temperature 95 DEG C obtains obtaining 100 object oyster biological zinc dry powder 0.65kg.
Embodiment 2
Another example utilizing oyster to prepare biological zinc method of the present invention, comprises the steps:
The oyster that lived by 40kg is shelled through cleaning, add the purified water of 1 times 5 DEG C, adding 0.15w/w% vigor is the lysozymes of 1.8 ten thousand U/g from egg white, the colloid mill being 2500r/min at rotating speed wears into fineness at 0.20mm, be placed in bioreactor, adding 0.5w/w% vigor is 250,000 U/g cellulases, the lipase of 2.5w/w% vigor to be 40,000 U/g fungal proteolytic enzyme and 0.8w/w% vigor be 100,000 U/g, temperature 42 DEG C, rotating speed 150r/min conditioned response was 800mm at centrifugal radius after 20 hours, rotating speed is 3500r/min centrifugation 15min, precipitum is for the preparation of oyster nutrition powder, centrifugate accurate filter filters, filtrate is 1:40 by blade diameter length ratio, linear flow speed is 25L/min, molecular sieve filled material is chromatographic isolation front resin transition, particle diameter is that after the molecular sieve of 0.20mm is separated to obtain molecular weight uniform concentration be that 20 ° of BX are rich in oyster biological zinc solution, temperature 65 DEG C, under vacuum absolute value 0.09 condition, Vacuum Concentration obtains the oyster biological zinc solution that is rich in of 40 ° of BX is 145 DEG C by EAT, and the atomizer drier of air outlet temperature 105 DEG C obtains obtaining 150 object oyster biological zinc dry powder 0.81kg.
Claims (5)
1. utilize oyster to prepare a biological zinc method, it is characterized in that, comprise the steps:
(1) oyster that lives is shelled through cleaning, and obtains fresh oyster fresh meat;
(2) doubly add the purified water of 3-5 DEG C by the 1-2 of fresh oyster fresh meat weight, add 0.05-0.15w/w%, vigor is the lysozyme of 1.5-1.8U/g source egg white, and wearing into fineness with colloid mill is 0.15-0.25mm slurry;
(3) slurry of step (2) gained is placed in bioreactor, add cellulase that 0.3-0.5w/w% vigor is 20-25 ten thousand U/g, 1.5-2.5w/w% vigor be 3-4 ten thousand U/g fungal proteolytic enzyme and 0.5-0.8w/w% vigor is 8-10U/g lipase, at temperature 35-42 DEG C, speed of agitator 150r/min conditioned response 10-20 hour, obtains enzymolysis liquid;
(4) by step (3) gained enzymolysis liquid through centrifugation 15min, obtain precipitum and centrifugate, precipitum for the preparation of oyster nutrition powder, centrifugate accurate filter filter obtain filtrate;
(5) obtaining molecular weight uniform concentration after step (4) gained filtrate being separated by molecular sieve separation equipment is that 15-20 ° of BX is rich in oyster biological zinc solution;
(6) be that 15-20 ° of BX is rich in oyster biological zinc solution at temperature 50-65 DEG C, Vacuum Concentration under vacuum absolute value 0.08-0.09 condition by step (5) concentration, obtain concentration be 35-40 ° of BX be rich in oyster biological zinc solution;
(7) be that 35-40 ° of BX is rich in oyster biological zinc solution and obtains 100-150 object oyster biological zinc dry powder by spray drying device by step (6) concentration.
2. according to claim 1ly utilize oyster to prepare biological zinc method, it is characterized in that, the described colloid mill rotating speed of step (2) is 1500-2200r/min.
3. according to claim 1ly utilize oyster to prepare biological zinc method, it is characterized in that, the centrifugation described in step (4) is 800mm at centrifugal radius, and rotating speed is carry out under the condition of 2500-3500r/min.
4. according to claim 1ly utilize oyster to prepare biological zinc method, it is characterized in that, the splitter blade diameter length ratio of step (5) molecular sieve equipment is 1:20-40, linear flow speed is 10-25L/min, molecular sieve particle diameter is 0.15-0.2mm, and molecular screen material is chromatographic isolation front resin transition.
5. according to claim 1ly utilize oyster to prepare biological zinc method, it is characterized in that, step (7) described spray drying device is atomizer drier, and EAT is 135-145 DEG C, air outlet temperature 95-105 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490572A (en) * | 2016-10-24 | 2017-03-15 | 泉州喜博生物保健食品有限公司 | The manufacture method of one seed oyster natural juice flavoring agent |
| CN106666536A (en) * | 2017-01-04 | 2017-05-17 | 广州聚澜健康产业研究院有限公司 | Biological zinc supplement |
| CN110467669A (en) * | 2019-07-22 | 2019-11-19 | 福建福瑞康信息技术有限公司 | The extracting method of Zinc metal sulfur protein zinc in one seed oyster |
| CN112280814A (en) * | 2020-10-29 | 2021-01-29 | 武汉芘芘薇莎生物科技有限公司 | High-content medium-low molecular weight konjac glucomannan, preparation method thereof and functional composition thereof |
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| CN1505983A (en) * | 2002-12-11 | 2004-06-23 | 张之中 | Biological enzymic hydrolysis process of oyster to prepare nutritious liquid and nutritious powder |
| CN101347460A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Oyster extract and method of preparing the same |
| CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
| CN102475266A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Preparation method of natural zinc-rich oyster powder |
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2015
- 2015-12-29 CN CN201511018396.3A patent/CN105476014B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1505983A (en) * | 2002-12-11 | 2004-06-23 | 张之中 | Biological enzymic hydrolysis process of oyster to prepare nutritious liquid and nutritious powder |
| CN101347460A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Oyster extract and method of preparing the same |
| CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
| CN102475266A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Preparation method of natural zinc-rich oyster powder |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490572A (en) * | 2016-10-24 | 2017-03-15 | 泉州喜博生物保健食品有限公司 | The manufacture method of one seed oyster natural juice flavoring agent |
| CN106666536A (en) * | 2017-01-04 | 2017-05-17 | 广州聚澜健康产业研究院有限公司 | Biological zinc supplement |
| CN110467669A (en) * | 2019-07-22 | 2019-11-19 | 福建福瑞康信息技术有限公司 | The extracting method of Zinc metal sulfur protein zinc in one seed oyster |
| CN112280814A (en) * | 2020-10-29 | 2021-01-29 | 武汉芘芘薇莎生物科技有限公司 | High-content medium-low molecular weight konjac glucomannan, preparation method thereof and functional composition thereof |
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