CN101347460A - Oyster extract and method of preparing the same - Google Patents
Oyster extract and method of preparing the same Download PDFInfo
- Publication number
- CN101347460A CN101347460A CNA2007101306809A CN200710130680A CN101347460A CN 101347460 A CN101347460 A CN 101347460A CN A2007101306809 A CNA2007101306809 A CN A2007101306809A CN 200710130680 A CN200710130680 A CN 200710130680A CN 101347460 A CN101347460 A CN 101347460A
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- oyster extract
- taurine
- oyster
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- preparation
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- 239000000284 extract Substances 0.000 title claims abstract description 58
- 241000237502 Ostreidae Species 0.000 title claims abstract description 48
- 235000020636 oyster Nutrition 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title description 8
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 64
- 229960003080 taurine Drugs 0.000 claims abstract description 32
- 150000001413 amino acids Chemical class 0.000 claims abstract description 28
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011701 zinc Substances 0.000 claims abstract description 24
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 21
- 238000002360 preparation method Methods 0.000 claims abstract description 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 19
- 229920001184 polypeptide Polymers 0.000 claims abstract description 14
- 235000013405 beer Nutrition 0.000 claims abstract description 13
- 235000013361 beverage Nutrition 0.000 claims abstract description 12
- 239000000654 additive Substances 0.000 claims abstract description 10
- 230000000996 additive effect Effects 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 7
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 3
- 235000001014 amino acid Nutrition 0.000 claims description 25
- 238000000703 high-speed centrifugation Methods 0.000 claims description 13
- 238000002604 ultrasonography Methods 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003456 ion exchange resin Substances 0.000 claims description 5
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 241000195474 Sargassum Species 0.000 claims description 4
- 230000000721 bacterilogical effect Effects 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229920005654 Sephadex Polymers 0.000 claims description 2
- 239000012507 Sephadex™ Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000013543 active substance Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000000047 product Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005714 functional activity Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000008521 reorganization Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- 229920002527 Glycogen Polymers 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000011436 enzymatic extraction method Methods 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention provides an oyster extract and a preparation method thereof. The extract contains biological zinc, taurine, amino acid and polypeptide; the content of amino acid and polypeptide is 5 to 23.4 percent, the content of biological zinc is 0.1 to 0.82 percent and the content of taurine is 0.5 to 8.4 percent. The preparation method combines enzymolysis and ultrasonic extraction and improves the preparation technology of oyster extract so as to obtain the oyster extract abundant in amino acid (polypeptide), biological zinc, taurine and other small-molecular functional active substances, which is suitable for the additive of beverage, particularly beer. The invention can not only effectively increase the extraction rate of the oyster extract, shorten the extraction time and save the cost, but also greatly improve the product quality and output.
Description
Technical field
The invention belongs to the marine organisms processing technique field, more particularly relate to extracting solution that extracts in the seed oyster and preparation method thereof.
Background technology
Concha Ostreae is a kind of precious shellfish, is rich in nutritional labelings such as glycogen, protein, taurine, aminoacid, vitamin, trace element, and particularly functional active components such as taurine wherein, aminoacid, biological zinc have been given very high value to it.Concha Ostreae can be improved immune function of human body, enhances metabolism, and has nutrition widely and medical value.It is the health care curative effect product that medical material can be used as food again that China's Ministry of Public Health approved is classified Concha Ostreae as first.In China, a lot of research work have been carried out in the application and development of Concha Ostreae, have wide commercial promise.
At present, Concha Ostreae extract research work and product are only paid close attention to the full trophic function aspect of Concha Ostreae mostly, and be less to the Study on Preparation of Concha Ostreae extract.And what adopt mostly in process of production is numerous and diverse isolation technics and process of tradition, makes the active component content in the Concha Ostreae extract relatively low, and contains a large amount of macromole foreign proteins, is not suitable for adding in beverage.
Summary of the invention
Purpose of the present invention; just be to overcome above-mentioned shortcoming and defect; adopt enzymolysis and supersonic extracting method to combine; improve the preparation technology of oyster extract; obtain being rich in the oyster extract of micromolecule functional activity materials such as aminoacid (polypeptide), biological zinc and taurine, suitable beverage is the interpolation of medicated beer particularly.
Oyster extract of the present invention contains biological zinc, taurine and aminoacid, polypeptide, wherein aminoacid and content of peptides be 5~23.4%, biological zinc content be 0.1~0.82% and content of taurine be 0.5~8.4%.
The preparation method of oyster extract of the present invention comprises the steps:
(1) Concha Ostreae is shelled cleanly, through ultrasonic extraction, behind the high speed centrifugation, gets supernatant, must be rich in the oyster extract of free amino acid and biological zinc;
(2) Concha Ostreae after step (1) is handled blends, adding distil water, and homogenate, through ultrasonic Treatment, behind protease hydrolysis, high speed centrifugation is got supernatant again.Must be rich in the oyster extract of aminoacid (comprising polypeptide) and taurine.Preferably ultrasound wave 60-80W extracted 10-20 minute, passed through high speed centrifugation 12000r/min again, and 10-15 minute, the protease hydrolysis through 0.5%;
(3) step (2) is rich in the oyster extract of taurine, through ion exchange resin, high-purity taurine;
(4) Concha Ostreae of above-mentioned steps (1), (2) and (3) resulting biological zinc, taurine and aminoacid (polypeptide) is extracted concentrated solution and reconfigure by a certain percentage, must can be applicable to the particularly oyster extract of beer additive of beverage;
The preparation method of oyster extract of the present invention can also comprise the steps:
(5) oyster extract after the reorganization is adopted kieselguhr and/or active carbon eliminating sargassum smell, carry out degerming, must be more suitable for being applied to the particularly oyster extract of beer additive of beverage by high speed centrifugation and/or bacteriological filtration film.
Ultrasound wave is a kind of high frequency mechanical wave, has molten, energy-conservation, the characteristics of high efficiency of expanding, and using ultrasound is strengthened extraction can extract small-molecule substance such as aminoacid and biological zinc in the Concha Ostreae efficiently.
Adopting protease that Concha Ostreae is carried out enzymolysis, can make the further enzymolysis of macro-molecular protein in the extract be low peptide and aminoacid, mainly is low peptide pattern, helps absorption by human body.
By ion exchange resin, because of containing sulfydryl on the taurine molecule, can carry out ion exchange, thereby obtain the high-purity natural taurine.
To the aminoacid that from Concha Ostreae, extracts; biological zinc and taurine carry out composite as required; promptly reconfigure by a certain percentage; making aminoacid and content of peptides is 5~23.4%; biological zinc content be 0.1~0.82% and content of taurine be 0.5~8.4%; then adopt kieselguhr or active carbon to carry out eliminating sargassum smell, carry out degerming in conjunction with high speed centrifugation or bacteriological filtration film at last, must can be applicable to the particularly oyster extract of beer additive of beverage.
Oyster extract of the present invention and preparation method thereof; adopt ultrasound wave and enzymatic extraction method to combine; improve the preparation technology of oyster extract; make oyster extract be rich in micromolecule functional activity materials such as aminoacid (polypeptide), biological zinc and taurine; the Concha Ostreae extract protein macromolecule that has solved traditional diamond-making technique production accounts for the overwhelming majority; and the necessary amino acid content of functional micromolecule biological zinc and taurine and human body is extremely low; as beverage additive, cause labile states such as precipitation, muddiness easily.
The oyster extract of using the present invention's preparation can be used as particularly beer additive use of beverage, and taurine and biological zinc content are very high in the extracting solution, and physiologically active is remarkable.In a word, the present invention not only can improve the extraction ratio of Concha Ostreae extract effectively, shortens extraction time, saves cost, also improves the quality and the output of product greatly.
The specific embodiment
The preparation process of the oyster extract of embodiment 1 is as follows:
(1) Concha Ostreae is shelled cleanly, extracts 10 minutes with ultrasound wave 60W then, passes through high speed centrifugation 12000r/min again, and 10 minutes, get supernatant, must be rich in the oyster extract of free amino acid and biological zinc;
(2) Concha Ostreae after step 1, after blending, adding distil water, homogenate, after ultrasound wave 60W handled, the protease hydrolysis through 0.5% passed through high speed centrifugation 12000r/min more again, 10 minutes, get supernatant, must be rich in the oyster extract of aminoacid (comprising polypeptide) and taurine;
(3),, get high-purity taurine through specific model resin Sephadex A25 ion exchange resin with the oyster extract that is rich in taurine in the step (2);
(4) Concha Ostreae of step (1), (2) and (3) resulting biological zinc, taurine and aminoacid (polypeptide) is extracted concentrated solution and reconfigure by a certain percentage, must can be applicable to the particularly oyster extract of beer additive of beverage according to different needs;
(5) to the oyster extract active carbon eliminating sargassum smell after the reorganization, carry out degerming, must be more suitable for being applied to the particularly oyster extract of beer additive of beverage by high speed centrifugation and bacteriological filtration film.
After the preparation process of above-mentioned oyster extract, the oyster extract that obtains contains biological zinc, taurine and aminoacid, polypeptide, aminoacid and content of peptides are 5~23.4%, biological zinc content be 0.1~0.82% and content of taurine be 0.5~8.4%.
In the oyster extract according to method preparation of the present invention, functional active components all is a small-molecule substance, does not have high molecular weight protein, helps the direct absorption of human body.Oyster extract is added in the medicated beer, not only increase its trophic function, can also make beer taste mellow, salubriouser.Because it does not contain macro-molecular protein, do not influence its stability and foaming properties after the interpolation in addition.Therefore, oyster extract is fit to add in medicated beer and other drink with function, and market prospect is wide.
The present invention describes with reference to embodiment, but protection domain is not limited thereto, and has those of ordinary skill and can pass through simple conversion in the technology of the present invention scope, and obtain the same technique effect of the present invention, equally in protection scope of the present invention.
Claims (7)
1. an oyster extract is characterized in that containing biological zinc and polypeptide, taurine and aminoacid.
2. oyster extract as claimed in claim 1 is characterized in that described aminoacid and content of peptides are 5~23.4%, biological zinc content be 0.1~0.82% and content of taurine be 0.5~8.4%.
3. the preparation method of oyster extract as claimed in claim 1 is characterized in that comprising the steps:
(1) Concha Ostreae is shelled cleanly, through ultrasonic extraction, behind the high speed centrifugation, gets supernatant, must be rich in the oyster extract of free amino acid and biological zinc;
(2) Concha Ostreae after step (1) is handled blends, adding distil water, and homogenate, through ultrasonic Treatment, behind protease hydrolysis, high speed centrifugation is got supernatant again, must be rich in the oyster extract of aminoacid (comprising polypeptide) and taurine;
(3) step (2) is rich in taurine oyster extract, through ion exchange resin, the high-purity taurine extracting solution;
(4) above-mentioned steps (1), (2) and (3) resulting Concha Ostreae of being rich in biological zinc, taurine and aminoacid (comprising polypeptide) are extracted concentrated solution and reconfigure by a certain percentage; making aminoacid and content of peptides is 5~23.4%; biological zinc content be 0.1~0.82% and content of taurine be 0.5~8.4%, must can be applicable to the particularly oyster extract of beer additive of beverage.
4. the preparation method of a kind of oyster extract as claimed in claim 3, it is characterized in that also comprising that (5) are to oyster extract employing kieselguhr and/or active carbon eliminating sargassum smell after recombinating, carry out degerming by high speed centrifugation and/or bacteriological filtration film, must be more suitable for the particularly oyster extract of beer additive of beverage.
5. as the preparation method of claim 3 or 4 described a kind of oyster extracts, it is characterized in that the ultrasound wave in the described step (1) is 60-80W, extraction time is 10-20 minute, high speed centrifugation 12000r/min, 10-15 minute.
6. as the preparation method of claim 3 or 4 described a kind of oyster extracts, it is characterized in that the ultrasound wave in the described step (2) is 60-80W, protease concentration is 0.5%, high speed centrifugation 12000r/min, 10-20 minute.
7. as the preparation method of claim 3 or 4 described a kind of oyster extracts, it is characterized in that described step (3) ion exchange resin is Sephadex A25.
Priority Applications (1)
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CNA2007101306809A CN101347460A (en) | 2007-07-16 | 2007-07-16 | Oyster extract and method of preparing the same |
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CNA2007101306809A CN101347460A (en) | 2007-07-16 | 2007-07-16 | Oyster extract and method of preparing the same |
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Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101870940A (en) * | 2010-05-28 | 2010-10-27 | 国家海洋局第一海洋研究所 | Method for preparing oyster active substance-containing beer |
CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
CN101982113A (en) * | 2010-09-26 | 2011-03-02 | 广东海洋大学 | Preparation method of crassostrea gigas meat antifatigue nutrient solution |
CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
CN102273633A (en) * | 2011-09-02 | 2011-12-14 | 唐作安 | Method for preparing oyster shell peptide oral liquid |
CN101755998B (en) * | 2009-10-30 | 2012-05-30 | 嵊泗海洋之星生物科技发展有限公司 | Preparation method of mussel active polypeptide |
CN102477004A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Method for extracting natural taurine from oysters by using ultrafiltration technology |
CN101601487B (en) * | 2009-07-09 | 2012-07-18 | 山东好当家海洋发展股份有限公司 | Shellfish protein beverage and preparation method thereof |
CN102660436A (en) * | 2012-05-24 | 2012-09-12 | 中国海洋大学 | Oyster yellow wine |
CN103932267A (en) * | 2014-04-30 | 2014-07-23 | 徐海菊 | Processing technique for preparing oyster meat nutritious powder by enzymolysis with assistance of ultrasound |
CN104544287A (en) * | 2013-10-12 | 2015-04-29 | 蔡庭守 | A method of preparing seafood active nutrition lyophilized powder |
CN105124522A (en) * | 2015-08-10 | 2015-12-09 | 中盐榆林盐化有限公司 | Taurine edible salt and preparation method thereof |
CN105433181A (en) * | 2015-12-31 | 2016-03-30 | 尹佳妮 | Instant beautiful millettia root drink and preparation method thereof |
CN105476014A (en) * | 2015-12-29 | 2016-04-13 | 广西潘达生物科技开发有限公司 | Method for preparing biological zinc from oysters |
RU2592407C1 (en) * | 2015-08-13 | 2016-07-20 | Олег Иванович Квасенков | Method for producing bread kvass |
RU2592409C1 (en) * | 2015-08-19 | 2016-07-20 | Олег Иванович Квасенков | Method for producing bread kvass |
CN106107900A (en) * | 2016-06-21 | 2016-11-16 | 中盐国本盐业有限公司 | Sal rich in taurine and preparation method thereof |
CN107815482A (en) * | 2017-11-02 | 2018-03-20 | 金华市艾力生物科技有限公司 | A kind of oyster peptide extracting method |
CN109486887A (en) * | 2018-10-25 | 2019-03-19 | 大连先先食品有限公司 | It is a kind of ultrasound and enzymatic hydrolysis coordinate system for oyster polypeptide method |
CN110467669A (en) * | 2019-07-22 | 2019-11-19 | 福建福瑞康信息技术有限公司 | The extracting method of Zinc metal sulfur protein zinc in one seed oyster |
CN111548873A (en) * | 2020-06-09 | 2020-08-18 | 中国海洋大学 | Preparation method of functional oyster beer |
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2007
- 2007-07-16 CN CNA2007101306809A patent/CN101347460A/en active Pending
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CN101601487B (en) * | 2009-07-09 | 2012-07-18 | 山东好当家海洋发展股份有限公司 | Shellfish protein beverage and preparation method thereof |
CN101755998B (en) * | 2009-10-30 | 2012-05-30 | 嵊泗海洋之星生物科技发展有限公司 | Preparation method of mussel active polypeptide |
CN101870940A (en) * | 2010-05-28 | 2010-10-27 | 国家海洋局第一海洋研究所 | Method for preparing oyster active substance-containing beer |
CN101870940B (en) * | 2010-05-28 | 2012-09-12 | 国家海洋局第一海洋研究所 | Method for preparing oyster active substance-containing beer |
CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
CN101914043B (en) * | 2010-07-23 | 2013-03-06 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
CN101982113A (en) * | 2010-09-26 | 2011-03-02 | 广东海洋大学 | Preparation method of crassostrea gigas meat antifatigue nutrient solution |
CN102477004A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Method for extracting natural taurine from oysters by using ultrafiltration technology |
CN102273633A (en) * | 2011-09-02 | 2011-12-14 | 唐作安 | Method for preparing oyster shell peptide oral liquid |
CN102660436A (en) * | 2012-05-24 | 2012-09-12 | 中国海洋大学 | Oyster yellow wine |
CN104544287A (en) * | 2013-10-12 | 2015-04-29 | 蔡庭守 | A method of preparing seafood active nutrition lyophilized powder |
CN103932267A (en) * | 2014-04-30 | 2014-07-23 | 徐海菊 | Processing technique for preparing oyster meat nutritious powder by enzymolysis with assistance of ultrasound |
CN105124522A (en) * | 2015-08-10 | 2015-12-09 | 中盐榆林盐化有限公司 | Taurine edible salt and preparation method thereof |
RU2592407C1 (en) * | 2015-08-13 | 2016-07-20 | Олег Иванович Квасенков | Method for producing bread kvass |
RU2592409C1 (en) * | 2015-08-19 | 2016-07-20 | Олег Иванович Квасенков | Method for producing bread kvass |
CN105476014B (en) * | 2015-12-29 | 2018-06-26 | 广西潘达生物科技开发股份有限公司 | A kind of method that biological zinc is prepared using oyster |
CN105476014A (en) * | 2015-12-29 | 2016-04-13 | 广西潘达生物科技开发有限公司 | Method for preparing biological zinc from oysters |
CN105433181A (en) * | 2015-12-31 | 2016-03-30 | 尹佳妮 | Instant beautiful millettia root drink and preparation method thereof |
CN106107900A (en) * | 2016-06-21 | 2016-11-16 | 中盐国本盐业有限公司 | Sal rich in taurine and preparation method thereof |
CN107815482A (en) * | 2017-11-02 | 2018-03-20 | 金华市艾力生物科技有限公司 | A kind of oyster peptide extracting method |
CN109486887A (en) * | 2018-10-25 | 2019-03-19 | 大连先先食品有限公司 | It is a kind of ultrasound and enzymatic hydrolysis coordinate system for oyster polypeptide method |
CN110467669A (en) * | 2019-07-22 | 2019-11-19 | 福建福瑞康信息技术有限公司 | The extracting method of Zinc metal sulfur protein zinc in one seed oyster |
CN111548873A (en) * | 2020-06-09 | 2020-08-18 | 中国海洋大学 | Preparation method of functional oyster beer |
CN111548873B (en) * | 2020-06-09 | 2021-06-15 | 中国海洋大学 | Preparation method of functional oyster beer |
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