CN104544287A - A method of preparing seafood active nutrition lyophilized powder - Google Patents
A method of preparing seafood active nutrition lyophilized powder Download PDFInfo
- Publication number
- CN104544287A CN104544287A CN201310476208.6A CN201310476208A CN104544287A CN 104544287 A CN104544287 A CN 104544287A CN 201310476208 A CN201310476208 A CN 201310476208A CN 104544287 A CN104544287 A CN 104544287A
- Authority
- CN
- China
- Prior art keywords
- filter residue
- marine product
- freeze
- supernatant
- carrying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 34
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 32
- 230000035764 nutrition Effects 0.000 title abstract description 8
- 235000014102 seafood Nutrition 0.000 title abstract 6
- 239000008176 lyophilized powder Substances 0.000 title abstract 4
- 239000006228 supernatant Substances 0.000 claims abstract description 33
- 230000001954 sterilising effect Effects 0.000 claims abstract description 22
- 238000005119 centrifugation Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 14
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 13
- 229960002675 xylitol Drugs 0.000 claims abstract description 13
- 235000010447 xylitol Nutrition 0.000 claims abstract description 13
- 239000000811 xylitol Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000000047 product Substances 0.000 claims description 54
- 239000012141 concentrate Substances 0.000 claims description 32
- 239000000843 powder Substances 0.000 claims description 23
- 230000008569 process Effects 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 21
- 241000251511 Holothuroidea Species 0.000 claims description 14
- 238000013467 fragmentation Methods 0.000 claims description 13
- 238000006062 fragmentation reaction Methods 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 11
- 150000001875 compounds Chemical class 0.000 claims description 10
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 102000057297 Pepsin A Human genes 0.000 claims description 5
- 108090000284 Pepsin A Proteins 0.000 claims description 5
- 229940111202 pepsin Drugs 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 235000015170 shellfish Nutrition 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 claims description 2
- 241000237502 Ostreidae Species 0.000 claims description 2
- 235000020636 oyster Nutrition 0.000 claims description 2
- 238000003757 reverse transcription PCR Methods 0.000 claims description 2
- 238000002390 rotary evaporation Methods 0.000 claims description 2
- 241000143060 Americamysis bahia Species 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 238000003672 processing method Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract 3
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 238000000227 grinding Methods 0.000 abstract 1
- 238000009413 insulation Methods 0.000 abstract 1
- 239000003755 preservative agent Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 210000002816 gill Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 1
- 240000001008 Dimocarpus longan Species 0.000 description 1
- 235000000235 Euphoria longan Nutrition 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 229930188089 Holothurin Natural products 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- MAWWITJOQDJRJF-ADBICINLSA-M holothurin Chemical compound [Na+].O[C@@H]1[C@@H](OC)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H](OC[C@H]([C@@H]3O)OS([O-])(=O)=O)O[C@@H]3C([C@H]4[C@](C=5[C@H]([C@@]6(CC[C@]7(O)[C@](C)([C@H]8OC(C)(C)CC8)OC(=O)[C@@]76[C@@H](O)C=5)C)CC4)(C)CC3)(C)C)O[C@@H]2C)O)O[C@H](C)[C@H]1O MAWWITJOQDJRJF-ADBICINLSA-M 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 241000238565 lobster Species 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000004768 organ dysfunction Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a method of preparing seafood nutrition lyophilized powder. The method includes the following steps: 1,mincing the seafood, carrying out uniform pulp grinding and crushing; separating the uniform pulp and obtaining a first supernatant, carrying out concentration and obtaining a concentrated solution 1; 2, adding water to the first residue and carrying out thermal insulation, carrying out centrifugal separation and obtaining a second supernatant, carrying out concentration and obtaining a concentrated solution 2; 3, adding water to a second residue and carrying out complex enzymatic treatment; 4, carrying out sterilization on the production of the step 3, adding xylitol into the production and carrying out complex reaction, carrying out sterilization and centrifugation and obtaining a third supernatant, carrying out concentration and obtaining a concentrated solution 3; 5, mixing the concentrated solution l, 2 and 3 and obtaining a mixed concentrated solution, carrying out sterilization, freeze-drying and obtaining a seafood active nutrition lyophilized powder. According to the method, the prepared seafood active nutrition lyophilized powder can be more easily absorbed by human body; no preservatives, hormones or other harmful substances are added in the processing. The method is a more advanced seafood processing method.
Description
Technical field
The present invention relates to marine product deep process technology field, particularly relate to a kind of preparation method of marine product active nutritional freeze-dried powder, as sea cucumber active nutritional freeze-dried powder.
Background technology
Traditional marine products processing goods mainly can be preserved for a long time, but can not directly use, need long-time bubble to send out and desalination during use, in making and use procedure, a large amount of marine product nutriment biologically active is scattered and disappeared, human body only absorbs a very little well-behaved active nutritional, causes serious waste of resources; Marine product dry powder in the market mainly contains two kinds of process technologies: 1, physical dryness breaking method, and the method fundamentally can not change the feature of protein macromolecule, and nutritional activities is low, and human absorptivity is low.2, biologic enzymolysis method, current technology, because the temperature of enzymolysis is too high, causes a large amount of inactivation of active nutritional; One step enzymatic isolation method, is only extracted the sub-fraction nutrition in marine product, a large amount of nutrition leak, and waste is serious.The present inventor, through testing for many years, works out a kind of marine products processing technology.
Summary of the invention
The object of the invention is the preparation method proposing a kind of marine product active nutritional freeze-dried powder, to overcome physical dryness comminuting method and biological enzymolysis sends out had inherent shortcoming.
In order to solve the problems of the technologies described above, present invention employs following technical scheme: a kind of preparation method of marine product active nutritional freeze-dried powder, comprises the following steps:
Step 1, will gill and homogenate is worn in the marine product cleaned rubbing, utilizes ultrasonic wave to carry out fragmentation to described homogenate; Centrifugation obtains the first supernatant and the first filter residue through the homogenate of fragmentation; Concentrated first supernatant obtains concentrate 1;
Step 2, adds the water of the first filter residue weight 2 ~ 4 times in the first filter residue, then at 40 ~ 60 DEG C of temperature, keeps 1 ~ 3h, and obtain the mixed liquor containing the first filter residue, centrifugation contains the mixed liquor of the first filter residue, obtains the second supernatant and the second filter residue; Concentrated second supernatant obtains concentrate 2;
Step 3, adds the water of the second filter residue weight 2 ~ 4 times in the second filter residue, and obtain the mixed liquor containing the second filter residue, then complex enzyme hydrolysis process contains the mixed liquor of the second filter residue; Particularly, meeting ferment treatment process described in is: adjustment contains pH to 7.8 ~ 8.5 of the mixed liquor of the second filter residue, and temperature is adjusted to 10 ~ 15 DEG C; The trypsase of the second filter residue weight 0.5% ~ 0.8%, enzymolysis 2 ~ 6h is added to containing in the mixed liquor of the second filter residue; Then regulate pH to 1 ~ 3, then add the pepsin of the second filter residue weight 0.8% ~ 0.9%, enzymolysis 2 ~ 6h.In above-mentioned steps, trypsase and pepsic addition all obtain through lot of experiments, and addition, in this scope, while obtaining better hydrolysis result, can not cause the enzyme owing to adding too many and cause cost to increase.
Step 4, carries out sterilizing to the mixed liquor containing the second filter residue after complex enzyme process, then adds xylitol wherein, make the mass concentration of xylitol be 8 ~ 12%; Subsequently reaction vessel pressure is adjusted to 500 ~ 800Mpa, pH value to 8.5 ~ 9.0, under the temperature conditions of 25 ~ 30 DEG C, react 30 ~ 90min obtain compound liquid, compound liquid described in centrifugation obtains the 3rd supernatant and the 3rd filter residue; Concentrated obtained concentrate 3 after 3rd supernatant pH value is adjusted to 7.0;
Step 5, mixes acquisition mixed concentrated liquid by concentrate 1, concentrate 2 and concentrate 3, the process of described mixed concentrated liquid sterilizing postlyophilization, obtained marine product active nutritional freeze-dried powder.
Preferably, when utilizing ultrasonic wave to carry out fragmentation to described homogenate in step 1, ultrasonic power is 60 ~ 90w, and the ultrasonic disruption time is 10 ~ 30min.Due to the particularity of marine product tissue, the power of ultrasonic disruption and selection of time need to carry out lot of experiments and just can draw.Ultrasonic power is greater than 90w can cause the too small of historrhexis, is unfavorable for follow-up centrifugation, and power be less than 60w can due to fragmentation thoroughly do not cause nutriment extract insufficient.Select the ultrasonic power of 60 ~ 90w, the broken time in conjunction with 10 ~ 30min is an optimum organization.
Preferably, the rotary evaporation that is evaporated to described in step 1, step 2 and step 3 concentrates.
Preferably, the sterilising conditions in step 4 and step 5 is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.
Preferably, the freeze drying in step 5 is carried out at-40 ~-20 DEG C.
Marine product of the present invention is fish marine product, shellfish by nest RT-PCR or shrimps marine product.Preferably, described marine product is sea cucumber, oyster or marine rainbow.
Compared with prior art, beneficial effect of the present invention is:
The invention provides a kind of step by step arithmetic, low temperature enzymolysis, low-temperature ultrahigh-pressure sterilizing, the active full nutrition freeze-dried powder of ultralow temperature freeze drying technology processing marine product, the nutrition of abundant reservation marine product and activity thereof, exist with Small molecular form, and the destruction of digestive juice to its activity can be hidden, make it by human body efficient absorption, be more conducive to giving full play to of marine product value, simultaneously, do not add the harmful materials such as any anticorrisive agent, hormone in processing, propose a kind of more advanced marine products processing method.
The marine product freeze-dried powder that the inventive method obtains, remain the whole nutritional labelings in marine product, people's physical efficiency efficient absorption is made to have bioactive Small molecular nutriment powder, in this way marine product is made medicine materical crude slice, capsule or electuary eat as health care product, maintain the peptide in marine product, the biologically active of the many kinds of substances such as acidic polysaccharose, solve the problem that tradition processing causes marine product nutriment inactivation and absorbs poor efficiency, edible, easy to carry, that there is alleviating physical fatigue, develop immunitypty, improve elasticity of skin and moisture content, beauty treatment is preserved youthful looks, the sea cucumber nutrient activated freeze dried powder of delaying senility function, be applicable to all kinds of crowd to eat.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, but not as a limitation of the invention.
Embodiment 1
Marine product used in embodiment 1 is fresh sea cucumber.
Step 1, gills 10kg marine product and cleans, and is rubbed by marine product and wears into homogenate, utilize ultrasonic wave (ultrasonic power is 60w, and the ultrasonic disruption time is 30min) to carry out fragmentation to described homogenate after adding the pure water of 3 times of weight; Centrifugation obtains the first supernatant and the first filter residue through the homogenate of fragmentation; Concentrated first supernatant obtains concentrate 1.
Step 2, adds the water of the first filter residue weight 2 times, then at 40 DEG C of temperature, keeps 3h in the first filter residue, and obtain the mixed liquor containing the first filter residue, centrifugation contains the mixed liquor of the first filter residue, obtains the second supernatant and the second filter residue; Concentrated second supernatant obtains concentrate 2;
Step 3, adds the water of the second filter residue weight 3 times in the second filter residue, and obtain the mixed liquor containing the second filter residue, then complex enzyme hydrolysis process contains the mixed liquor of the second filter residue; Concrete enzymolysis process is: the pH to 7.8 regulating the mixed liquor containing the second filter residue, and temperature is adjusted to 15 DEG C; The trypsase of the second filter residue weight 0.5%, enzymolysis 2 ~ 6h is added to containing in the mixed liquor of the second filter residue; Then regulate pH to 1, then add the pepsin of the second filter residue weight 0.9%, enzymolysis 2 ~ 6h.
Step 4, carry out sterilizing to the mixed liquor containing the second filter residue after complex enzyme process, sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then add xylitol wherein, make the mass concentration of xylitol be 8 ~ 12%; Subsequently reaction vessel pressure is adjusted to 500 ~ 800Mpa, pH value to 8.5, under the temperature conditions of 25 DEG C, react 90min obtain compound liquid and (utilize the principle of xylitol not easily digested liquid digestion containing sea cucumber mucopolysaccharide, holothurin, sea cucumber molecular peptide, xylitol, make most of active material be carried into intestinal absorption smoothly and enter blood, active nutritional material absorbing efficiency is improved greatly.), compound liquid described in centrifugation obtains the 3rd supernatant and the 3rd filter residue; Concentrated obtained concentrate 3 after 3rd supernatant pH value is adjusted to 7.0.
Step 5, mixes acquisition mixed concentrated liquid by concentrate 1, concentrate 2 and concentrate 3, described mixed concentrated liquid sterilizing, and sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then freeze drying process is carried out at-30 DEG C, obtained marine product active nutritional freeze-dried powder.
Sea cucumber, people have found the precious nutritive value of sea cucumber from ancient times, as the food materials of integration of drinking and medicinal herbs, are described as first of " ocean eight delicacies ", nutritious and balanced and reasonable, and the kind of nutritional labeling is up to kind more than 50.Supplementary Amplifications of the Compendium of Materia Medica is recorded: sea cucumber, sweet-salty, kidney tonifying, and beneficial marrow, takes the photograph urine, and impotence is treated in establishing-Yang, its benefit warm in nature, sufficient enemy's ginseng, therefore named sea cucumber." book on Chinese herbal medicine asks former ": sea cucumber moisturizing viscera, grow essence profit products, nourishing yin and supplementing blood, strong positive, moisturize, menstruation regulating, nourishing the fetus, diuresis.Traditional Chinese Medicine thinks that sea cucumber is the high-grade invigorant of rare tonifying kidney and benefiting sperm, nourishing yin and nourishing blood, tonifying both YIN and YANG.It can kidney-replenishing, again can nourishing kidney-yin, has tonifying both YIN and YANG, the effect of reinforcing vital energy.Modern medicine thinks that sea cucumber nutrient has powerful reparative regeneration function, effectively can repair the cell of human body damaged, recovers and improves organ dysfunction.
Embodiment 2
Marine product used in embodiment 2 is fresh longan shellfish.
Step 1, gills 10kg marine product and cleans, and is rubbed by marine product and wears into homogenate, utilize ultrasonic wave (ultrasonic power is 90w, and the ultrasonic disruption time is 10min) to carry out fragmentation to described homogenate after adding the pure water of 3 times of weight; Centrifugation obtains the first supernatant and the first filter residue through the homogenate of fragmentation; Concentrated first supernatant obtains concentrate 1.
Step 2, adds the water of the first filter residue weight 4 times, then at 60 DEG C of temperature, keeps 1h in the first filter residue, and obtain the mixed liquor containing the first filter residue, centrifugation contains the mixed liquor of the first filter residue, obtains the second supernatant and the second filter residue; Concentrated second supernatant obtains concentrate 2;
Step 3, adds the water of the second filter residue weight 4 times in the second filter residue, and obtain the mixed liquor containing the second filter residue, then complex enzyme hydrolysis process contains the mixed liquor of the second filter residue; Concrete enzymolysis process is: the pH to 8.5 regulating the mixed liquor containing the second filter residue, and temperature is adjusted to 12 DEG C; The trypsase of the second filter residue weight 0.8%, enzymolysis 2 ~ 6h is added to containing in the mixed liquor of the second filter residue; Then regulate pH to 3, then add the pepsin of the second filter residue weight 0.8%, enzymolysis 2 ~ 6h.
Step 4, carry out sterilizing to the mixed liquor containing the second filter residue after complex enzyme process, sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then add xylitol wherein, make the mass concentration of xylitol be 8 ~ 12%; Subsequently reaction vessel pressure is adjusted to 500 ~ 800Mpa, pH value to 9.0, reacts 60min and obtain compound liquid under the temperature conditions of 30 DEG C, compound liquid described in centrifugation obtains the 3rd supernatant and the 3rd filter residue; Concentrated obtained concentrate 3 after 3rd supernatant pH value is adjusted to 7.0.
Step 5, mixes acquisition mixed concentrated liquid by concentrate 1, concentrate 2 and concentrate 3, described mixed concentrated liquid sterilizing, and sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then freeze drying process is carried out at-40 DEG C, obtained marine product active nutritional freeze-dried powder.
Embodiment 3
Marine product used in embodiment 3 is fresh lobster.
Step 1, gills 10kg marine product and cleans, and is rubbed by marine product and wears into homogenate, utilize ultrasonic wave (ultrasonic power is 80w, and the ultrasonic disruption time is 15min) to carry out fragmentation to described homogenate after adding the pure water of 3 times of weight; Centrifugation obtains the first supernatant and the first filter residue through the homogenate of fragmentation; Concentrated first supernatant obtains concentrate 1.
Step 2, adds the water of the first filter residue weight 3 times, then at 50 DEG C of temperature, keeps 2h in the first filter residue, and obtain the mixed liquor containing the first filter residue, centrifugation contains the mixed liquor of the first filter residue, obtains the second supernatant and the second filter residue; Concentrated second supernatant obtains concentrate 2;
Step 3, adds the water of the second filter residue weight 2 times in the second filter residue, and obtain the mixed liquor containing the second filter residue, then complex enzyme hydrolysis process contains the mixed liquor of the second filter residue; Concrete enzymolysis process is: the pH to 8 regulating the mixed liquor containing the second filter residue, and temperature is adjusted to 10 DEG C; The trypsase of the second filter residue weight 0.9%, enzymolysis 2 ~ 6h is added to containing in the mixed liquor of the second filter residue; Then regulate pH to 2, then add the pepsin of the second filter residue weight 0.9%, enzymolysis 2 ~ 6h.
Step 4, carry out sterilizing to the mixed liquor containing the second filter residue after complex enzyme process, sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then add xylitol wherein, make the mass concentration of xylitol be 8 ~ 12%; Subsequently reaction vessel pressure is adjusted to 500 ~ 800Mpa, pH value to 8.5 ~ 9.0, reacts 40min and obtain compound liquid under the temperature conditions of 28 DEG C, compound liquid described in centrifugation obtains the 3rd supernatant and the 3rd filter residue; Concentrated obtained concentrate 3 after 3rd supernatant pH value is adjusted to 7.0.
Step 5, mixes acquisition mixed concentrated liquid by concentrate 1, concentrate 2 and concentrate 3, described mixed concentrated liquid sterilizing, and sterilising conditions is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.Then freeze drying process is carried out at-20 DEG C, obtained marine product active nutritional freeze-dried powder.
Above embodiment is only exemplary embodiment of the present invention, and be not used in restriction the present invention, protection scope of the present invention is defined by the claims.Those skilled in the art can in essence of the present invention and protection domain, and make various amendment or equivalent replacement to the present invention, this amendment or equivalent replacement also should be considered as dropping in protection scope of the present invention.
Claims (8)
1. a preparation method for marine product active nutritional freeze-dried powder, said method comprising the steps of:
Step 1, will gill and homogenate is worn in the marine product cleaned rubbing, utilizes ultrasonic wave to carry out fragmentation to described homogenate; Centrifugation obtains the first supernatant and the first filter residue through the homogenate of fragmentation; Concentrated first supernatant obtains concentrate 1;
Step 2, adds the water of the first filter residue weight 2 ~ 4 times in the first filter residue, then at 40 ~ 60 DEG C of temperature, keeps 1 ~ 3h, and obtain the mixed liquor containing the first filter residue, centrifugation contains the mixed liquor of the first filter residue, obtains the second supernatant and the second filter residue; Concentrated second supernatant obtains concentrate 2;
Step 3, adds the water of the second filter residue weight 2 ~ 4 times in the second filter residue, and obtain the mixed liquor containing the second filter residue, then complex enzyme hydrolysis process contains the mixed liquor of the second filter residue;
Step 4, carries out sterilizing to the mixed liquor containing the second filter residue after complex enzyme process, then adds xylitol wherein, make the mass concentration of xylitol be 8 ~ 12%; Subsequently reaction vessel pressure is adjusted to 500 ~ 800Mpa, pH value to 8.5 ~ 9.0, under the temperature conditions of 25 ~ 30 DEG C, react 30 ~ 90min obtain compound liquid, compound liquid described in centrifugation obtains the 3rd supernatant and the 3rd filter residue; Concentrated obtained concentrate 3 after 3rd supernatant pH value is adjusted to 7.0;
Step 5, mixes acquisition mixed concentrated liquid by concentrate 1, concentrate 2 and concentrate 3, the process of described mixed concentrated liquid sterilizing postlyophilization, obtained marine product active nutritional freeze-dried powder.
2. the preparation method of marine product active nutritional freeze-dried powder according to claim 1, is characterized in that, meets ferment treatment process and be described in step 3: pH to 7.8 ~ 8.5 regulating the mixed liquor containing the second filter residue, and temperature is adjusted to 10 ~ 15 DEG C; The trypsase of the second filter residue weight 0.5% ~ 0.8%, enzymolysis 2 ~ 6h is added to containing in the mixed liquor of the second filter residue; Then regulate pH to 1 ~ 3, then add the pepsin of the second filter residue weight 0.8% ~ 0.9%, enzymolysis 2 ~ 6h.
3. the preparation method of marine product active nutritional freeze-dried powder according to claim 1, is characterized in that, when utilizing ultrasonic wave to carry out fragmentation to described homogenate in step 1, ultrasonic power is 60 ~ 90w, and the ultrasonic disruption time is 10 ~ 30min.
4. the preparation method of marine product active nutritional freeze-dried powder according to claim 1, is characterized in that, the rotary evaporation that is evaporated to described in step 1, step 2 and step 3 concentrates.
5. the preparation method of marine product active nutritional freeze-dried powder according to claim 1, is characterized in that, the sterilising conditions in step 4 and step 5 is: reaction vessel pressure is adjusted to 800 ~ 1000MPa, and temperature is adjusted to 15 ~ 20 DEG C, keeps 20 ~ 40min.
6. the preparation method of marine product active nutritional freeze-dried powder according to claim 1, it is characterized in that, the freeze drying in step 5 is carried out at-40 ~-20 DEG C.
7. the preparation method of the marine product active nutritional freeze-dried powder according to any one of claim 1-6, is characterized in that, described marine product is fish marine product, shellfish by nest RT-PCR or shrimps marine product.
8. the preparation method of the marine product active nutritional freeze-dried powder according to any one of claim 1-6, is characterized in that, described marine product is sea cucumber, oyster or marine rainbow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310476208.6A CN104544287A (en) | 2013-10-12 | 2013-10-12 | A method of preparing seafood active nutrition lyophilized powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310476208.6A CN104544287A (en) | 2013-10-12 | 2013-10-12 | A method of preparing seafood active nutrition lyophilized powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104544287A true CN104544287A (en) | 2015-04-29 |
Family
ID=53062208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310476208.6A Pending CN104544287A (en) | 2013-10-12 | 2013-10-12 | A method of preparing seafood active nutrition lyophilized powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104544287A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839553A (en) * | 2015-05-22 | 2015-08-19 | 遵义市杨老大米粉厂 | longyan shell rice flour and preparation method thereof |
| CN106191187A (en) * | 2016-08-03 | 2016-12-07 | 北海明杰科技有限公司 | A kind of method utilizing lobster shell to prepare anti-oxidation peptide |
| CN106666478A (en) * | 2016-12-29 | 2017-05-17 | 徐静 | Preparation method of zymolytic lyophilized powder |
| CN115553417A (en) * | 2022-09-28 | 2023-01-03 | 河北尚谷食品科技有限公司 | Preparation process of high-quality dry rice flour |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478421A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Seacucumbus whole powder food and its preparation method |
| CN1871945A (en) * | 2005-06-01 | 2006-12-06 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN101347460A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Oyster extract and method of preparing the same |
| CN101356985A (en) * | 2008-09-04 | 2009-02-04 | 中国海洋大学 | Production method of direct-edible raw sea-slug |
| CN101411523A (en) * | 2008-11-14 | 2009-04-22 | 钱武刚 | Method for extracting original liquid of trepang by ultrasonic wave |
-
2013
- 2013-10-12 CN CN201310476208.6A patent/CN104544287A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478421A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Seacucumbus whole powder food and its preparation method |
| CN1871945A (en) * | 2005-06-01 | 2006-12-06 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
| CN101347460A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Oyster extract and method of preparing the same |
| CN101356985A (en) * | 2008-09-04 | 2009-02-04 | 中国海洋大学 | Production method of direct-edible raw sea-slug |
| CN101411523A (en) * | 2008-11-14 | 2009-04-22 | 钱武刚 | Method for extracting original liquid of trepang by ultrasonic wave |
Non-Patent Citations (3)
| Title |
|---|
| 余杰等: "酶法制备牡蛎抗氧化肽研究", 《中国海洋药物》 * |
| 夏远景: "超高压食品处理工艺基础研究", 《中国博士学位论文全文数据库 工程科技I辑》 * |
| 赵伟等: "超高压处理对牡蛎超微结构、组分及蛋白质变性的影响", 《食品与发酵工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839553A (en) * | 2015-05-22 | 2015-08-19 | 遵义市杨老大米粉厂 | longyan shell rice flour and preparation method thereof |
| CN106191187A (en) * | 2016-08-03 | 2016-12-07 | 北海明杰科技有限公司 | A kind of method utilizing lobster shell to prepare anti-oxidation peptide |
| CN106666478A (en) * | 2016-12-29 | 2017-05-17 | 徐静 | Preparation method of zymolytic lyophilized powder |
| CN115553417A (en) * | 2022-09-28 | 2023-01-03 | 河北尚谷食品科技有限公司 | Preparation process of high-quality dry rice flour |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106551391B (en) | A kind of pilose antler deep working method | |
| CN104206641B (en) | A kind of giant salamander polypeptide powder and preparation method thereof | |
| CN107653290A (en) | A kind of preparation method of sturgeon protein peptides | |
| CN105200105A (en) | Preparation method of oyster protein liver protecting peptide preparation | |
| CN103750419B (en) | Extraction method for natural instant oyster powder | |
| CN104082725B (en) | A kind of snake gall honey freeze-dried powder and preparation method thereof | |
| CN104544287A (en) | A method of preparing seafood active nutrition lyophilized powder | |
| CN102224949A (en) | Preparation technology of total nutrient powder (peptide) of oysters | |
| KR100840156B1 (en) | Making method of sam-gae-thang juice and sam-gae-thang using them | |
| CN103099248A (en) | Method for preparing oyster active component | |
| CN106913857A (en) | A kind of extra large stichopus japonicus active peptide and preparation method thereof | |
| CN107574215A (en) | A kind of preparation method of pollen pini active peptide | |
| CN103988912A (en) | Making method of selenium-rich black tea with milk | |
| CN106901114A (en) | A kind of celery juice beverage and preparation method thereof | |
| CN104082728B (en) | A kind of edible fly larva honey freeze-dried powder and preparation method thereof | |
| CN110357954A (en) | Easily absorb the collagen and its preparation process for improving skin | |
| CN109662277A (en) | A method of the instant lyophilized powder of red bayberry is prepared using myrica ruba marc | |
| CN105395444A (en) | Method for preparing oyster facial mask | |
| KR101126379B1 (en) | Manufacturing method of the honey ginger tea granule honey and ginger with the main material | |
| CN103652898A (en) | Solid composition containing holothurian and chlorella pyrenoidosa and preparation method thereof | |
| CN113817791A (en) | Formula and preparation method of sea cucumber and oyster extract micromolecular peptide | |
| CN106235071A (en) | The processing technique of former dry Stichopus japonicus | |
| CN105901128A (en) | Composite selenium-rich rice milk slice with effects of tonifying spleen and boosting essence | |
| CN102512351B (en) | Sea cucumber extract used for skin care cosmetics and processing method | |
| CN105851745A (en) | Hami melon vegetable protein beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20151106 Address after: Hangzhou City, Zhejiang province 310051 Binjiang District West Street No. 567 Jiangling Road, building 2, room 1212 Applicant after: Hangzhou Shanhai Tong health Polytron Technologies Inc Address before: 100039 Beijing city Haidian District West Hill Road No. 5 home 11-2102 Applicant before: Cai Tingshou Applicant before: Qian Renkan |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20161020 Address after: 3502, room 3, building 310000, Qianjiang international Times Plaza, Jianggan District, Hangzhou, Zhejiang Applicant after: Shanhai Tong Holdings Ltd Address before: Hangzhou City, Zhejiang province 310051 Binjiang District West Street No. 567 Jiangling Road, building 2, room 1212 Applicant before: Hangzhou Shanhai Tong health Polytron Technologies Inc |
|
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150429 |
|
| RJ01 | Rejection of invention patent application after publication |