CN105454314A - Hatching egg cleaning disinfectant and preparation method thereof - Google Patents
Hatching egg cleaning disinfectant and preparation method thereof Download PDFInfo
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- CN105454314A CN105454314A CN201510724504.2A CN201510724504A CN105454314A CN 105454314 A CN105454314 A CN 105454314A CN 201510724504 A CN201510724504 A CN 201510724504A CN 105454314 A CN105454314 A CN 105454314A
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- 238000004140 cleaning Methods 0.000 title claims abstract description 33
- 230000012447 hatching Effects 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000000645 desinfectant Substances 0.000 title abstract description 9
- 235000013601 eggs Nutrition 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000008367 deionised water Substances 0.000 claims abstract description 9
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000006184 cosolvent Substances 0.000 claims abstract description 7
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 23
- 235000014103 egg white Nutrition 0.000 claims description 23
- 210000000969 egg white Anatomy 0.000 claims description 23
- 231100000167 toxic agent Toxicity 0.000 claims description 22
- 239000003440 toxic substance Substances 0.000 claims description 22
- 238000005202 decontamination Methods 0.000 claims description 21
- 230000003588 decontaminative effect Effects 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 17
- 230000000844 anti-bacterial effect Effects 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 12
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 9
- 244000223014 Syzygium aromaticum Species 0.000 claims description 9
- 241000205585 Aquilegia canadensis Species 0.000 claims description 8
- 235000001405 Artemisia annua Nutrition 0.000 claims description 8
- 240000000011 Artemisia annua Species 0.000 claims description 8
- 241000050051 Chelone glabra Species 0.000 claims description 8
- 241000758769 Houttuynia Species 0.000 claims description 8
- 235000013717 Houttuynia Nutrition 0.000 claims description 8
- 239000010231 banlangen Substances 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 8
- 239000004094 surface-active agent Substances 0.000 claims description 8
- 241000218691 Cupressaceae Species 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 4
- 239000006286 aqueous extract Substances 0.000 claims description 3
- 239000000469 ethanolic extract Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 3
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical class CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 claims description 2
- ILRSCQWREDREME-UHFFFAOYSA-N dodecanamide Chemical compound CCCCCCCCCCCC(N)=O ILRSCQWREDREME-UHFFFAOYSA-N 0.000 claims description 2
- 239000003651 drinking water Substances 0.000 claims description 2
- 235000020188 drinking water Nutrition 0.000 claims description 2
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 2
- 239000008213 purified water Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 14
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 230000000249 desinfective effect Effects 0.000 abstract description 2
- 239000006260 foam Substances 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 238000007654 immersion Methods 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 241000607764 Shigella dysenteriae Species 0.000 description 5
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 229940007046 shigella dysenteriae Drugs 0.000 description 5
- 239000003206 sterilizing agent Substances 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 239000012459 cleaning agent Substances 0.000 description 4
- 238000002791 soaking Methods 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 238000003958 fumigation Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000712461 unidentified influenza virus Species 0.000 description 3
- 241000193738 Bacillus anthracis Species 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000191963 Staphylococcus epidermidis Species 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 208000037386 Typhoid Diseases 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 229940065181 bacillus anthracis Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 206010013023 diphtheria Diseases 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 239000012286 potassium permanganate Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 201000008297 typhoid fever Diseases 0.000 description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical class CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000001716 anti-fugal effect Effects 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- BLUAFEHZUWYNDE-NNWCWBAJSA-N artemisinin Chemical compound C([C@](OO1)(C)O2)C[C@H]3[C@H](C)CC[C@@H]4[C@@]31[C@@H]2OC(=O)[C@@H]4C BLUAFEHZUWYNDE-NNWCWBAJSA-N 0.000 description 1
- 229930101531 artemisinin Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002233 benzalkonium bromide Drugs 0.000 description 1
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003559 chemosterilizing effect Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- -1 decocts Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 229930183339 qinghaosu Natural products 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K43/00—Testing, sorting or cleaning eggs ; Conveying devices ; Pick-up devices
- A01K43/005—Cleaning, washing of eggs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a hatching egg cleaning disinfectant and a preparation method thereof. The hatching egg cleaning disinfectant comprises a natural antibiotic traditional Chinese medicinal material, a nonionic surfactant, a cosolvent and deionized water. The hatching egg cleaning disinfectant has no irritation, toxic or side effects, has strong cleaning and disinfecting effects, and can effectively prevent vertical propagation of various diseases of breeding birds, the preparation method has the advantages of simplicity and easy operation, and the hatching egg cleaning disinfectant is suitable for immersion disinfection and foam disinfection of hatching eggs and cleaning of the hatching eggs, and can effectively inhibit vertical propagation of the diseases.
Description
Technical field
The present invention relates to a kind of cleaning and sterilizing agent and preparation method thereof, particularly kind egg white decontamination toxic agent and preparation method thereof.
Background technology
Plant egg and answer cleaning and sterilizing after collection, be attached to the various bacteriums on the wet eggshell just given birth to, fungi and virus breeding very fast, invade in egg and affect hatching effect, and may by transmission to chick, the generation of cleaning and sterilizing work to the various disease of prevention chick carrying out kind of egg is significant.
Current kind of egg disinfection way is mainly fumigation and soaking disinfection, fumigation mainly formalin and potassium permanganate with the use of, will ventilate after fumigation 1h 24h, and waste time and energy, residual toxicity is not easily removed, and in operating process, danger is very large.Formalin and potassium permanganate react acutely in addition, and corrosivity is strong, has very large excitant, have certain limitation age to the embryo of equipment and kind egg.The kind egg of soaking disinfection to cleaning of benzalkonium bromide and Operand can reach good Disinfection Effect, but there is no cleaning function, to the kind egg easily residual germ being stained with the dirts such as ight soil, bloodstain, bedding and padding, sterilization is not thorough, and long soaking time also can destroy kind of an egg collagen layer.In addition, these chemosterilants have certain stimulation and toxicity at present, and use procedure drug residue has certain harm to kind of an egg, human body and environment.The pure middle pharmacopoeia egg disinfectant property of medicine is gentle and do not have clean dirt effect, is subject to the impacts such as the organic matter such as ight soil, bloodstain.
Summary of the invention
For above deficiency, the invention provides a kind of excitant little, have no side effect, cleaning, disinfecting power is strong, to the cleaning and sterilizing agent without harm of human body and environment, be issued to the effect of Rapid Cleaning sterilization at general operation environment, ensure human body and environment without harm simultaneously.
A described kind egg white decontamination toxic agent, comprises the component of following parts by weight:
Described natural antibacterial medicinal material is selected from one or more combinations in cordate houttuynia, Radix Isatidis, the root of large-flowered skullcap, sweet wormwood, cloves, golden cypress, honeysuckle.
Described natural antibacterial medicinal material parts by weight are: cordate houttuynia 10-12 part, Radix Isatidis 5-7 part, root of large-flowered skullcap 6-7 part, sweet wormwood 9-15 part, cloves 8-12 part, golden cypress 4-7 part, honeysuckle 5-8 part.
Described natural antibacterial medicinal material parts by weight are: cordate houttuynia 10 parts, Radix Isatidis 5 parts, the root of large-flowered skullcap 7 parts, sweet wormwood 12 parts, cloves 8 parts, golden cypress 7 parts, honeysuckle 6 parts.
Described non-ionic surface active agent is one or more combinations in Octyl glucoside, ethoxylated dodecyl alcohol, lauric acid amide of ethanol.
Described cosolvent is one or both in propane diols, polyethylene glycol.
Described water is drinking water, purified water or deionized water.
Its preparation method realizes as follows:
(1) accurately take natural antibacterial traditional Chinese medicine in mass ratio, remove impurity, co-grinding, adds 4-8 times of ethanol, soak extraction 2-5h under 20-40 DEG C of condition, and isolated by filtration residue obtains solution A (ethanol extract);
(2) by ethanol evaporate to dryness in residue, soak 3-5h under adding 5-10 times of deionized water normal temperature, decoct 1-2.5h, isolated by filtration residue, obtains B solution (aqueous extract);
(3) solution A mixed with B solution, remove impurity with 0.2-0.5 μm of membrane filtration, being concentrated into density is 1.02-1.11g/cm
3obtain natural antibacterial Chinese medical extract.
(4) natural antibacterial Chinese medical extract, non-ionic surface active agent, cosolvent are mixed in deionized water and make finished product.
Described kind of egg white decontamination toxic agent is used for the cleaning of kind of egg;
Described kind of egg white decontamination toxic agent is used for the sterilization of kind of egg.
The invention has the beneficial effects as follows:
The present invention is led to prescription and is selected natural antibacterial traditional Chinese medicine, non-ionic surface active agent, cosolvent and deionized water, occurring in nature is biodegradable, pollution-free, nonirritant, to kind of an egg, operating personnel and environment without any harm, to equipment without particular/special requirement, safe, healthy, simple to operate.
Containing compositions such as decanoy acetaldehydes in cordate houttuynia, all there is certain inhibitory action to staphylococcus aureus, staphylococcus albus, shigella dysenteriae and influenza virus etc.
Radix Isatidis has effect to various bacteria, and its extract is to hay bacillus, staphylococcus aureus, and sarcine, Escherichia coli, typhoid bacillus, paratyphoid A bacillus, shigella dysenteriae, bacillus enteritidis and courageous and upright streptococcus etc. have inhibitory action.
The root of large-flowered skullcap all has inhibitory action to multiple gram-positive bacteria, Gram-negative bacteria, fungi and influenza virus.
Sweet wormwood has stronger bacteriostasis to staphylococcus, micrococcus catarrhalis, bacillus anthracis, diphtheria bacillus, also certain inhibitory action is had, in addition the effect of qinghaosu antimycotic, resisiting influenza virus in addition to staphylococcus aureus, Pseudomonas aeruginosa, shigella dysenteriae, tuberculosis stalk bacterium etc.
Honeysuckle has very strong resisting pathogenic microbes effect, to various pathogens as staphylococcus aureus, hemolytic streptococcus, Escherichia coli, shigella dysenteriae, comma bacillus, typhoid bacillus, paratyphosum Bacterium etc. all have certain inhibitory action.
The alcohol extract of cloves all has inhibitory action to diphtheria bacillus, bacillus anthracis, paratyphosum Bacterium, shigella dysenteriae, staphylococcus aureus, staphylococcus albus, comma bacillus.The water of cloves, alcohol and ether leachate and its volatile oil, all have obvious antifungic action to pathogenic fungus.
Several kinds of Chinese medicinal materials combination supplements mutually, makes it all have very strong killing action to common various bacteriums, fungi and virus.Traditional Chinese medicine first first uses alcohol extract at normal temperatures in extraction, then residue is soaked in water, decocts, extracts, active ingredient can be made to extract fully, and non-ionic surface active agent has certain bactericidal action and stronger cleaning function, makes sterilization more thoroughly more complete.Preparation method's processing method of the present invention is simple, do not need special equipment, utilization rate of raw materials is high, all has stronger killing effect to various bacterium, fungi and virus, be applicable to the cleaning of the soaking disinfection of kind of egg, foam disinfectant and kind egg, effectively can suppress the vertical transmission of disease.
Embodiment
Embodiment 1
Kind egg white decontamination toxic agent and preparation method thereof, prescription and the preparation method of available feature embodiments are below illustrated.Component is as shown in the table:
Accurately take each component according to above-mentioned prescription, adopt following preparation method to obtain:
(1) cordate houttuynia, Radix Isatidis, the root of large-flowered skullcap, sweet wormwood, cloves, golden cypress, honeysuckle are removed impurity, co-grinding;
(2) under normal temperature condition, first use 6 times of alcohol extract 5h, be separated residue, filter, remove impurity and obtain solution A (ethanol extract);
(3) by ethanol evaporate to dryness in residue, under adding 5 times of deionized water normal temperature, soak 3h, decoct 1.5h, be separated residue, filter, remove impurity and obtain B solution (aqueous extract);
(4) solution A mixed with B solution, remove impurity with 0.5 μm of membrane filtration, being concentrated into density is 1.05g/cm
3obtain natural antibacterial Chinese medical extract;
(5) natural antibacterial Chinese medical extract, non-ionic surface active agent and cosolvent are mixed, add water and mend to recipe quantity.
Embodiment 2 cleaning and sterilizing is tested
1 material
Experimental group 1: the present invention plants egg white decontamination toxic agent, prepares by above-mentioned prescription 1;
Experimental group 2: the present invention plants egg white decontamination toxic agent, prepares by above-mentioned prescription 2;
Experimental group 3: the present invention plants egg white decontamination toxic agent, prepares by above-mentioned prescription 3;
Experimental group 4: the present invention plants egg white decontamination toxic agent, prepares by above-mentioned prescription 4;
Control group 1: prepare by a kind of patent " Chinese medicinal extract disinfectant " method;
Control group 2: running water.
2 methods
2.1 grouping
Test is divided into 4 experimental group, 2 control groups totally six groups, often organizes 20 kind eggs.Experimental group adopts the present invention to plant egg white and washes toxic agent embodiment 1-4 prescription, by cleaning and sterilizing agent dilute with water 40 times, rinses, take out after immersing hatching eggs 15min with soak.Control group 1 adopts the pure middle pharmacopoeia egg disinfectant of certain brand kind commercially available, by description of product dilution, rinses, take out after immersing hatching eggs 15min with soak.Rinse with soak after control group 2 picks up from water immersing hatching eggs 15min, take out.
2.2 sampling
Kind of an egg extending shaft is divided into a district, Liang Ge region, b district, wipes with sterile cotton and pick physiological saline at kind of an egg surface a district 2x2cm
23 times are repeatedly embrocated, as sample before cleaning and sterilizing in areal extent; Sample after as cleaning and sterilizing is sampled in b region by same method after cleaning and sterilizing.
2.3 observation on cleaning
Observe according to kind of egg surface smut remaining quantity, the degree of adhesion of dirt and wiping effect judge cleaning performance.Cleaning performance is marked with A, B, C, D, and A represents degree of cleaning minimum (dirt remaining quantity without visible change, strong adhesion, wiping is without significant change); B (dirt remaining quantity reduces or adhesive force reduces, and wiping can obviously reduce dirt remaining quantity); C (dirt remaining quantity reduces, and adhesive force is low, and wiping can remove dirt): D represents degree of cleaning the highest (thoroughly, egg clean surface, without wiping in dirt cleaning).
2.4 sterilizing effect observing
Cotton after sampling is wiped in the 10ml sterile saline dropped into containing 10% sodium thiosulfate, jolting 80 times, get washing lotion plain agar method and pour into cultivation and carry out count plate (37 DEG C, 24h), often organize sterilization rate and get its mean, calculating sterilizing rate.
3 results
Each group of table 1 is to kind of an egg white wash disinfection effect
As can be seen from the results: cleaning and sterilizing agent of the present invention has very strong cleaning, Disinfection Effect.Prescription 1-4 sample is compared with control group, and cleaning performance is obvious, remarkable to the elimination effect of ight soil, bloodstain and bedding and padding; The present invention's each prescription sample sterilizing rate is all higher than control group, and wherein prescription 2 Disinfection Effect is best, and sterilizing rate reaches 99.9%.Control group 1 selects pure Chinese medicine antiseptic, and owing to not having infiltration and the stripping effect of non-ionic surface active agent, sterilizing rate is starkly lower than experimental group.
Above embodiments of the invention have been described in detail, but described content is only preferred embodiment of the present invention, not in order to limit the present invention.All make in application range of the present invention any amendment, equivalent to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. a kind egg white decontamination toxic agent, comprises the component of following parts by weight:
2. a kind egg white decontamination toxic agent according to claim 1, is characterized in that described natural antibacterial medicinal material is selected from one or more combinations in cordate houttuynia, Radix Isatidis, the root of large-flowered skullcap, sweet wormwood, cloves, golden cypress, honeysuckle.
3. a kind egg white decontamination toxic agent according to claim 2, is characterized in that described natural antibacterial medicinal material parts by weight are: cordate houttuynia 10-12 part, Radix Isatidis 5-7 part, root of large-flowered skullcap 6-7 part, sweet wormwood 9-15 part, cloves 8-12 part, golden cypress 4-7 part, honeysuckle 5-8 part.
4. a kind egg white decontamination toxic agent according to claim 3, is characterized in that described natural antibacterial medicinal material parts by weight are: cordate houttuynia 10 parts, Radix Isatidis 5 parts, the root of large-flowered skullcap 7 parts, sweet wormwood 12 parts, cloves 8 parts, golden cypress 7 parts, honeysuckle 6 parts.
5. a kind egg white decontamination toxic agent according to claim 1, is characterized in that described non-ionic surface active agent is one or more combinations in Octyl glucoside, ethoxylated dodecyl alcohol, lauric acid amide of ethanol.
6. a kind egg white decontamination toxic agent according to claim 1, is characterized in that described cosolvent is one or both in propane diols, polyethylene glycol.
7. a kind egg white decontamination toxic agent according to claim 1, is characterized in that described water is one in drinking water, purified water, deionized water or combination.
8. a kind egg white decontamination toxic agent according to claim 1, is characterized in that its preparation method realizes as follows:
(1) accurately take natural antibacterial traditional Chinese medicine in mass ratio, remove impurity, co-grinding, adds 4-8 times of ethanol, soak extraction 2-5h under 20-40 DEG C of condition, and isolated by filtration residue obtains solution A (ethanol extract);
(2) by ethanol evaporate to dryness in residue, soak 3-5h under adding 5-10 times of deionized water normal temperature, decoct 1-2.5h, isolated by filtration residue, obtains B solution (aqueous extract);
(3) solution A mixed with B solution, remove impurity with 0.2-0.5 μm of membrane filtration, being concentrated into density is 1.02-1.11g/cm
3obtain natural antibacterial Chinese medical extract;
(4) natural antibacterial Chinese medical extract, non-ionic surface active agent, cosolvent are mixed in deionized water and make finished product.
9. according to claim a kind of egg white decontamination toxic agent, it is characterized in that the cleaning and sterilizing of described kind of egg white decontamination toxic agent for kind of egg, its using method is by described kind of egg white decontamination toxic agent dilute with water 40 times, rinses, take out after immersing hatching eggs 15min with soak.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510724504.2A CN105454314A (en) | 2015-10-29 | 2015-10-29 | Hatching egg cleaning disinfectant and preparation method thereof |
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| CN105850792A (en) * | 2016-04-07 | 2016-08-17 | 五河县顺鹏农副产品有限公司 | Incubation method of Chinese soft-shell turtle eggs |
| CN106135131A (en) * | 2016-07-31 | 2016-11-23 | 普定县远山林下养鸡场 | A kind of Gallus Domesticus sylvan life cultural method |
| CN106386668A (en) * | 2016-08-31 | 2017-02-15 | 李宏强 | Efficient method for breeding and freely raising high-quality Jing Yuan chickens |
| CN106473021A (en) * | 2016-09-30 | 2017-03-08 | 界首市英琪养殖专业合作社 | A kind of sweet-smelling health care tempering egg |
| CN106472406A (en) * | 2016-09-30 | 2017-03-08 | 界首市英琪养殖专业合作社 | A kind of efficient hatching method of goose |
| CN106852344A (en) * | 2016-11-22 | 2017-06-16 | 戴琪 | A kind of preparation method of safty of drinking water disinfectant |
| CN107646881A (en) * | 2017-10-10 | 2018-02-02 | 广州市澳米环保科技有限公司 | A kind of disinfectant and preparation method thereof |
| CN111387135A (en) * | 2020-03-13 | 2020-07-10 | 北京家禽育种有限公司 | Method for systematically monitoring and purifying pseudomonas aeruginosa in hatching process |
| CN112205403A (en) * | 2020-10-20 | 2021-01-12 | 华心生物科技(深圳)有限公司 | Chicken embryo egg disinfectant based on vaccine production and method |
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| CN105850792A (en) * | 2016-04-07 | 2016-08-17 | 五河县顺鹏农副产品有限公司 | Incubation method of Chinese soft-shell turtle eggs |
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| CN106852344A (en) * | 2016-11-22 | 2017-06-16 | 戴琪 | A kind of preparation method of safty of drinking water disinfectant |
| CN107646881A (en) * | 2017-10-10 | 2018-02-02 | 广州市澳米环保科技有限公司 | A kind of disinfectant and preparation method thereof |
| CN111387135A (en) * | 2020-03-13 | 2020-07-10 | 北京家禽育种有限公司 | Method for systematically monitoring and purifying pseudomonas aeruginosa in hatching process |
| CN111387135B (en) * | 2020-03-13 | 2021-10-29 | 北京家禽育种有限公司 | Method for systematically monitoring and purifying pseudomonas aeruginosa in hatching process |
| CN112205403A (en) * | 2020-10-20 | 2021-01-12 | 华心生物科技(深圳)有限公司 | Chicken embryo egg disinfectant based on vaccine production and method |
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