CN105452450B - 培养癌干细胞的方法 - Google Patents

培养癌干细胞的方法 Download PDF

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CN105452450B
CN105452450B CN201480045085.3A CN201480045085A CN105452450B CN 105452450 B CN105452450 B CN 105452450B CN 201480045085 A CN201480045085 A CN 201480045085A CN 105452450 B CN105452450 B CN 105452450B
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cancer stem
stem cells
cells
cell culture
gel
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CN105452450A (zh
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栗泽元一
山下敦
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Agency for Science Technology and Research Singapore
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
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  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201480045085.3A 2013-06-18 2014-06-18 培养癌干细胞的方法 Expired - Fee Related CN105452450B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SG201304704-8 2013-06-18
SG2013047048 2013-06-18
PCT/SG2014/000290 WO2014204406A1 (en) 2013-06-18 2014-06-18 Method of culturing cancer stem cells

Publications (2)

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CN105452450A CN105452450A (zh) 2016-03-30
CN105452450B true CN105452450B (zh) 2020-07-07

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CN201480045085.3A Expired - Fee Related CN105452450B (zh) 2013-06-18 2014-06-18 培养癌干细胞的方法

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US (1) US20160145580A1 (enExample)
EP (1) EP3011016B1 (enExample)
JP (1) JP6552487B2 (enExample)
CN (1) CN105452450B (enExample)
SG (1) SG11201510462QA (enExample)
WO (1) WO2014204406A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11834671B2 (en) 2017-02-20 2023-12-05 National University Corporation Hokkaido University Method of producing cancer stem cells
WO2018168955A1 (ja) * 2017-03-15 2018-09-20 メカノジェニック株式会社 細胞のｉｎ ｖｉｖｏでの特性を反映した細胞の反応の評価
WO2019226120A1 (en) * 2018-05-23 2019-11-28 Agency For Science, Technology And Research A tumour cell culture system and a method of preparing a tumour cell culture system

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2010138082A1 (en) * 2009-05-29 2010-12-02 Agency For Science, Technology And Research Flavonoid hydrogel

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WO2006010066A2 (en) * 2004-07-09 2006-01-26 The Cleveland Clinic Foundation Hydroxyphenyl cross-linked macromolecular network and applications thereof
JP2007297360A (ja) * 2006-05-08 2007-11-15 Ihara Suisan Kk ハイドロゲル、その製造方法およびその用途
CN101855337B (zh) * 2007-06-29 2017-10-31 船木真理 用于进行干细胞调节的软凝胶系统
WO2009116951A2 (en) * 2008-03-17 2009-09-24 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8691206B2 (en) * 2008-05-06 2014-04-08 Agency For Science, Technology And Research Formation of hydrogel in the presence of peroxidase and low concentration of hydrogen peroxide
WO2009148405A1 (en) * 2008-06-05 2009-12-10 Agency For Science, Technology And Research Formation of hydrogel in the presence of peroxidase and low concentration of hydrogen peroxide
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
SG168430A1 (en) * 2009-07-22 2011-02-28 Agency Science Tech & Res Molecular signature of human lung cancer initiating cells
EP2498830B1 (en) * 2009-11-11 2016-09-21 University of Twente, Institute for Biomedical Technology and Technical Medicine (MIRA) Dextran-hyaluronic acid based hydrogels
WO2011059326A2 (en) * 2009-11-11 2011-05-19 University Of Twente, Institute For Biomedical Technology And Technical Medicine (Mira) Hydrogels based on polymers of dextran tyramine and tyramine conjugates of natural polymers
US8853162B2 (en) * 2011-05-11 2014-10-07 Agency For Science, Technology And Research Interpenetrating polymer network comprising fibrin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010138082A1 (en) * 2009-05-29 2010-12-02 Agency For Science, Technology And Research Flavonoid hydrogel

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Hyaluronan regulates cell behavior: a potential niche matrix for stem cell;Solis MA et al;《Biochem Res Int》;20120212;第2012卷;1-11 *
Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics;Guvendiren M et al;《Nat Commun》;20120424;第3卷;1-9 *

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Publication number Publication date
WO2014204406A1 (en) 2014-12-24
JP2016523079A (ja) 2016-08-08
SG11201510462QA (en) 2016-01-28
CN105452450A (zh) 2016-03-30
EP3011016B1 (en) 2019-08-07
EP3011016A4 (en) 2016-11-30
EP3011016A1 (en) 2016-04-27
US20160145580A1 (en) 2016-05-26
JP6552487B2 (ja) 2019-07-31

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