JP6552487B2 - 癌幹細胞を培養する方法 - Google Patents

癌幹細胞を培養する方法 Download PDF

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JP6552487B2
JP6552487B2 JP2016521251A JP2016521251A JP6552487B2 JP 6552487 B2 JP6552487 B2 JP 6552487B2 JP 2016521251 A JP2016521251 A JP 2016521251A JP 2016521251 A JP2016521251 A JP 2016521251A JP 6552487 B2 JP6552487 B2 JP 6552487B2
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glycosaminoglycan
cancer stem
cells
kpa
gel
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JP2016523079A5 (enExample
JP2016523079A (ja
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元一 栗沢
元一 栗沢
山下　敦
敦 山下
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Agency for Science Technology and Research Singapore
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2016521251A 2013-06-18 2014-06-18 癌幹細胞を培養する方法 Expired - Fee Related JP6552487B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SG2013047048 2013-06-18
SG201304704-8 2013-06-18
PCT/SG2014/000290 WO2014204406A1 (en) 2013-06-18 2014-06-18 Method of culturing cancer stem cells

Publications (3)

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JP2016523079A JP2016523079A (ja) 2016-08-08
JP2016523079A5 JP2016523079A5 (enExample) 2017-06-15
JP6552487B2 true JP6552487B2 (ja) 2019-07-31

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JP2016521251A Expired - Fee Related JP6552487B2 (ja) 2013-06-18 2014-06-18 癌幹細胞を培養する方法

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US (1) US20160145580A1 (enExample)
EP (1) EP3011016B1 (enExample)
JP (1) JP6552487B2 (enExample)
CN (1) CN105452450B (enExample)
SG (1) SG11201510462QA (enExample)
WO (1) WO2014204406A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11834671B2 (en) 2017-02-20 2023-12-05 National University Corporation Hokkaido University Method of producing cancer stem cells
JPWO2018168955A1 (ja) * 2017-03-15 2020-01-16 メカノジェニック株式会社 細胞のｉｎ ｖｉｖｏでの特性を反映した細胞の反応の評価
WO2019226120A1 (en) * 2018-05-23 2019-11-28 Agency For Science, Technology And Research A tumour cell culture system and a method of preparing a tumour cell culture system

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102382308B (zh) * 2004-07-09 2014-04-16 克利夫兰临床基金会 羟基苯交联大分子网络及其应用
JP2007297360A (ja) * 2006-05-08 2007-11-15 Ihara Suisan Kk ハイドロゲル、その製造方法およびその用途
CN101855337B (zh) * 2007-06-29 2017-10-31 船木真理 用于进行干细胞调节的软凝胶系统
EP2479260B1 (en) * 2008-03-17 2016-01-06 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8691206B2 (en) * 2008-05-06 2014-04-08 Agency For Science, Technology And Research Formation of hydrogel in the presence of peroxidase and low concentration of hydrogen peroxide
JP5563563B2 (ja) * 2008-06-05 2014-07-30 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ ペルオキシダーゼおよび低濃度の過酸化水素の存在下でのヒドロゲルの形成方法
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
SG168430A1 (en) * 2009-07-22 2011-02-28 Agency Science Tech & Res Molecular signature of human lung cancer initiating cells
SG176286A1 (en) * 2009-05-29 2012-01-30 Agency Science Tech & Res Flavonoid hydrogel
WO2011059326A2 (en) * 2009-11-11 2011-05-19 University Of Twente, Institute For Biomedical Technology And Technical Medicine (Mira) Hydrogels based on polymers of dextran tyramine and tyramine conjugates of natural polymers
WO2011059325A2 (en) * 2009-11-11 2011-05-19 University Of Twente, Institute For Biomedical Technology And Technical Medicine (Mira) Dextran-hyaluronic acid based hydrogels
US8853162B2 (en) * 2011-05-11 2014-10-07 Agency For Science, Technology And Research Interpenetrating polymer network comprising fibrin

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Publication number Publication date
EP3011016A4 (en) 2016-11-30
CN105452450A (zh) 2016-03-30
EP3011016B1 (en) 2019-08-07
US20160145580A1 (en) 2016-05-26
CN105452450B (zh) 2020-07-07
EP3011016A1 (en) 2016-04-27
SG11201510462QA (en) 2016-01-28
WO2014204406A1 (en) 2014-12-24
JP2016523079A (ja) 2016-08-08

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