CN105433049A - Preparation method of live bait for replacing artemia larva - Google Patents

Preparation method of live bait for replacing artemia larva Download PDF

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Publication number
CN105433049A
CN105433049A CN201510926774.1A CN201510926774A CN105433049A CN 105433049 A CN105433049 A CN 105433049A CN 201510926774 A CN201510926774 A CN 201510926774A CN 105433049 A CN105433049 A CN 105433049A
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CN
China
Prior art keywords
artemia
preparation
adults
fry
biological feed
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Pending
Application number
CN201510926774.1A
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Chinese (zh)
Inventor
王秀华
杨冰
刘莉
黄倢
张庆利
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Application filed by Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences filed Critical Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority to CN201510926774.1A priority Critical patent/CN105433049A/en
Publication of CN105433049A publication Critical patent/CN105433049A/en
Pending legal-status Critical Current

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Abstract

The invention relates to a preparation method of a live bait for replacing an artemia larva. The preparation method comprises taking artemia adults in a halogen pool or a salt field, washing the artemia adults by clear fresh water to remove silt and other impurities, carrying out drainage, heating the artemia adults to a temperature of 60-90 DEG C, keeping the temperature for 20-50min for disinfection, fast cooling the artemia adults to a temperature of 4-5 DEG C, according to demands, crushing the disinfected artemia adults through a beating machine to obtain microparticles with different sizes and directly feeding the microparticles to fry or carrying out vacuum-pumping split charging and then refrigeration storage at a temperature of -20 DEG C or selling. The live bait for replacing the artemia larva can be widely used in fish fry and young shrimp breeding and can be used as feed of fish fry and young shrimp and replace artemia larvas so that a fry breeding cost is greatly reduced and fry breeding production benefits are improved. The preparation method has simple processes.

Description

A kind of preparation method of biological feed of alternative artemia larvae
Technical field
The present invention relates to marine organisms bait technology of preparing, specifically a kind of preparation method of biological feed of alternative artemia larvae.
Background technology
China is aquaculture big country, and aquaculture annual production occupies the first place of countries in the world aquaculture production, and aquaculture has become the important component part of China's fishery.Developing rapidly of culture fishery, has benefited from the abundance supply of aquatic product sprout, the seed enormous amount such as the fish shrimp crab that annual China cultivates.In the Seedling production process of fish shrimp crab, need a large amount of artemia larvaes as the feed in seedling stage, to meet the demand of growth of seedling, therefore slag oxygenation substantial appreciation of prices is caused, increase breeding enterprise cost, slag oxygenation price 50-60 per ton ten thousand yuans at present for hatching artemia larvae, the feedstuff for worm of high price brings great financial burden to aquatic fry growing enterprise.Development can reduce product price for this reason, can meet again the bait of cultivated animals larval growth demand, become the active demand of industry development.
In view of northern China coastal area and inland brine lake have large-area high halogen pond for evaporating brine or salt pan, wherein growth has a large amount of adult ' Luchung ' insects.Because halogen worm is nutritious, fish for and be often used as multiple marine cultured animal cultivation phase feed afterwards, improve the speed of growth of cultivated animals, strengthen premunition.This adult ' Luchung ' insect low price, about 5000 yuan per ton is one of percentage of slag oxygenation price, therefore research and development utilize this adult ' Luchung ' insect, substitute artemia larvae, namely substitute artemia larvae with a kind of biological feed, greatly will alleviate the production cost of aquatic fry growing and enterprise thereof, increase economic benefit.
Summary of the invention
The object of this invention is to provide a kind of preparation method of biological feed of alternative artemia larvae, to make up the deficiencies in the prior art.
The present invention is to solve the problem that aquiculture animal seedling stage is in short supply with halogen worm, price is high, therefore a large amount of adult ' Luchung ' insect is obtained from halogen lake salt Tanaka, through cleaning, physical sterilization detoxification, then broken adult ' Luchung ' insect, make it to become meet different growing stages needs size, make finally by packing the series of processes such as freezing.
Therefore, preparation method of the present invention: the adult ' Luchung ' insect collecting halogen pond or salt Tanaka, eluriate with clear water and remove silt and other impurity, drain away the water, 60 ?90 DEG C that heats maintains 20 ?50min, is cooled to 4 ?5 DEG C afterwards.According to the demand of the different breeding animal young, pulverize the particle diameter preparing different size with beater respectively, comprise 0.05-0.1mm, 0.1mm-0.5mm, 0.5mm-1mm, 1mm-2mm, 2mm-3mm, again through vacuumizing packaging after packing, depositing or selling for-20 DEG C.
Biological feed prepared by the present invention is applied to feed in prawn, the nursery of the fish young in seedling stage with alternative artemia larvae.
During use, according to the age in days of the different breeding animal young, choose the alternative feed of goods as artemia larvae of different-grain diameter halogen worm, feeding volume carries out determining or reference product operation instruction according to putting seedling density.
Therefore, the present invention be directly by the wild adult ' Luchung ' insect that obtains from halogen pond and salt Tanaka through cleaning, physical sterilization detoxification, the biological feed product being finally broken into different breeding animal demand in seedling stage yardstick carrys out alternative artemia larvae feed.Obviously, the present invention compares the preparation of artemia larvae, its method is reasonable, technique simple, and abundance is gathered materials on the spot, and cost is low, use simple, this is because the preparation of traditional artemia larvae needs by artemia ovum hatching, in artemia ovum hatching process, needs certain equipment and maintains certain incubation temperature, also need to use seawater simultaneously, great inconvenience is brought to the nursery of non-coastal area.Field grown halogen worm in addition, often carries some pathogenic microorganisms.Product of the present invention, through sterilization, can directly be thrown something and fed, use very safe, convenient, be of high nutritive value, adopt low temperature sterilization again, the nutriment be rich in is not destroyed, comprehensive nutrition, the demand in each growth period of aquatic livestock can be met completely, produce for nursery and save substantial contribution.
Detailed description of the invention
The invention will be further described by the following examples.
Embodiment 1
Apply the goods that particle diameter of the present invention is 0.05-0.1mm and 0.1-0.5mm, substitute artemia larvae and cultivate Penaeus Vannmei.
1 material
1.1 test the shrimp young and seedling managements
Environment of Litopenaeus vannamei Low (Litopenaeusvannamei) zoea 1 phase (Z1) 3,000,000 tail.Cultivation temperature 31 DEG C, natural seawater salinity 28, adopts 6, conventional seedbed system pond, effective water body 10m in every pond 3, continuous charge in cultivating process, throws something and feeds 6 every day, day feeding volume be 2g/ ten thousand tail.Particle diameter of the present invention of throwing something and feeding during flea shape is that the feed of 0.05-0.1mm substitutes halogen worm, particle diameter of the present invention of throwing something and feeding between acanthosoma is that the feed of 0.1-0.5-mm substitutes halogen worm, control group is is all thrown something and fed artemia larvae, test group with all add the black prawn slice of identical amount in control group breeding water body as auxiliary material, every day every 4 grams, ten thousand tail.
2 test methods
2.1 test groupings: the broken group (test group) of adult ' Luchung ' insect, artemia larvae group (control group) are established in test, often group establish three parallel, often organize 500,000 tails.
2.2 Environment of Litopenaeus vannamei Low larvae survival rates measure
Test first 3 days, add up each group of prawn by zoea to the survival number after mysis metamorphosis, calculate prawn larvae survival rate.Test latter 3 days, add up each group of prawn by mysis to the survival number after young shrimp young transformation, calculating prawn larvae survival rate.Survival rate (%)=whole end number/initial mantissa × 100.
3 result of the tests
3.1 flea shapes are to survival rate during oppossum shrimp
Prawn is cultivated after 3d, and each group prawn to oppossum shrimp 1 phase (M1), adds up the survival number of each group of mysis, calculating prawn survival rate (table 1) through development by metamorphosis, known test group prawn survival rate and control group survival rate difference little.Test group prawn larvae survival rate is 94.3%, and control group juvenile prawn survival rate is 95.3%, and statistical analysis shows two group differences not significantly (p>0.05).
Table 1 cultivates and terminates each test group prawn survival rate
Fig.1Survivalrateofexperimentalshrimpindifferentgroups
Survival rate during 3.2 oppossum shrimp 1 phases to young 1 phase of shrimp
Prawn is cultivated after 6d, and each group prawn to young shrimp 1 phase (P1), adds up the survival number of each group of young shrimp, calculating prawn survival rate (table 2) through development by metamorphosis, known test group prawn survival rate and control group survival rate difference little.The young shrimp survival rate of test group is 75.3%, and the young shrimp survival rate of control group is 73%, and statistical analysis shows two group differences not significantly (p>0.05).
Table 2 cultivates and terminates each test group prawn survival rate
Fig.1Survivalrateofexperimentalshrimpindifferentgroups
Embodiment 2
Apply the goods that particle diameter of the present invention is 1mm-2mm, substitute artemia larvae and cultivate japonicus seedling.
1 material
1.1 test the shrimp young and seedling managements
Young shrimp 5 phase (P5) of japonicus (penaeusjapanicus), long (0.56 ± 0.11) cm of body, totally 180 ten thousand tails are evenly distributed in 6 conventional ponds.Effective water body 50m in every pond 3, continuous charge in cultivating process, cultivation temperature 25 DEG C, natural seawater salinity 28, throws something and feeds 3 every day, day feeding volume be 50g/ ten thousand tail.Control group is is all thrown something and fed artemia larvae, and other management parameters are completely the same.
2 test methods
2.1 test groupings: the broken group (test group) of adult ' Luchung ' insect, artemia larvae group (control group) are established in test, often group establish three parallel, often group puts seedling 300,000 tail.Culture experiment totally 10 days.
2.2 Penaeus japonicus post-larvae survival rates measure
Cultivate after 10 days, add up each group of prawn by survival rate during young shrimp 5 phase to young 15 phase of shrimp and the long growth rate of prawn body.Survival rate (%)=whole end number/initial mantissa × 100, during the long growth rate of body (%)=(during off-test, during body length-on-test, body is long)/on-test, body grows × 100.
3 result of the tests
3.1 Penaeus japonicus post-larvae survival rates
After prawn culturing 10d, each group of prawn grows into P15 by p5, add up the survival number of each group of young shrimp, calculate prawn survival rate (table 1), known test group prawn larvae survival rate is 99.0%, control group juvenile prawn survival rate is 98.7%, and test group prawn survival rate slightly improves than contrast composition motility rate.Statistical analysis shows two group differences not significantly (p>0.05).Show to use large halogen worm goods of the present invention to substitute artemia larvae.
Table 1 cultivates and terminates each test group prawn survival rate
Fig.1Survivalrateofexperimentalshrimpindifferentgroups
The long growth rate of 3.2 Penaeus japonicus post-larvae body
After shrimp seed rearing 10d, the long growth rate of each group prawn body is in table 2, and the long growth rate of known test group prawn body is a little more than the long growth rate of control group body.The long growth rate of the young shrimp body of test group is 164.8%, and the long growth rate of the young shrimp body of control group is 164.3%, and statistical analysis shows two group differences not significantly (p>0.05).Show to use large halogen worm goods of the present invention to substitute artemia larvae.
The long rate of Penaeus japonicus post-larvae body after table 2 cultivates 10 days
Fig.1Survivalrateofexperimentalshrimpindifferentgroups

Claims (5)

1. the preparation method of the biological feed of an alternative artemia larvae, it is characterized in that, collect the adult ' Luchung ' insect of halogen pond or salt Tanaka, silt and other impurity is removed after eluriating with clean fresh water, drain away the water, sterilization of heating, is cooled fast to 4-5 DEG C afterwards, the timely particle diameter cooled halogen worm being crushed to different size with pulverizer according to demand again, has pulverized and has directly been used as bait feeding afterwards.
2. the preparation method of biological feed as claimed in claim 1, it is characterized in that, the sterilising temp of heating of above-mentioned adult ' Luchung ' insect is 60-90 DEG C, holds time at 20-50min.
3. the preparation method of biological feed as claimed in claim 1, it is characterized in that, the cooled adult ' Luchung ' insect of above-mentioned sterilizing is broken into particle diameter and is respectively 0.05-0.1mm, 0.1mm-0.5mm, 0.5mm-1mm, 1mm-2mm and 2mm-3mm.
4. the preparation method of biological feed as claimed in claim 1, is characterized in that, the halogen worm after above-mentioned pulverizing through vacuumizing packaging ,-20 DEG C of freezen protective.
5. the biological feed that described in claim 1 prepared by the preparation method of biological feed is applied to feed in prawn, the nursery of the fish young in seedling stage with alternative artemia larvae.
CN201510926774.1A 2015-12-14 2015-12-14 Preparation method of live bait for replacing artemia larva Pending CN105433049A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109619367A (en) * 2018-12-04 2019-04-16 天津海友佳音生物科技股份有限公司 A kind of method that artemia nauplii saves

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Publication number Priority date Publication date Assignee Title
CN1073836A (en) * 1992-01-04 1993-07-07 南京市粮食科学研究设计所 The halogen worm does (powder) production Technology
CN1200881A (en) * 1997-05-29 1998-12-09 刘新嘉 Fine processing technology for dried whole fairy-shrimps
CN1246301A (en) * 1999-09-03 2000-03-08 新疆精河县艾比湖生物制品有限责任公司 Process for preparing dewatered bittern worm
CN101779604A (en) * 2009-12-25 2010-07-21 广东海洋大学 Method for culturing seedlings of Portunus tritubereulatus in north and breeding Portunus tritubereulatus in south
CN102893927A (en) * 2011-07-26 2013-01-30 上海市水产研究所 Feeding method of controllable bait for fries of Takifugu flavidus
CN103141426A (en) * 2013-03-28 2013-06-12 中国水产科学研究院黄海水产研究所 Method for efficiently breeding blue crabs
CN104431506A (en) * 2014-11-28 2015-03-25 中国科学院南海海洋研究所 Method for preparing frozen sea horse feed

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109619367A (en) * 2018-12-04 2019-04-16 天津海友佳音生物科技股份有限公司 A kind of method that artemia nauplii saves

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