CN105378107A - 多重甲基化-特异性扩增系统和方法 - Google Patents

多重甲基化-特异性扩增系统和方法 Download PDF

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Publication number
CN105378107A
CN105378107A CN201480027133.6A CN201480027133A CN105378107A CN 105378107 A CN105378107 A CN 105378107A CN 201480027133 A CN201480027133 A CN 201480027133A CN 105378107 A CN105378107 A CN 105378107A
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China
Prior art keywords
nucleic acid
amplification
methylation
amplicon
dna
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CN201480027133.6A
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Chinese (zh)
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E.格拉纳多斯
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Abbott Laboratories
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Abbott Laboratories
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201480027133.6A 2013-03-14 2014-03-13 多重甲基化-特异性扩增系统和方法 Pending CN105378107A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361782370P 2013-03-14 2013-03-14
US61/782370 2013-03-14
PCT/US2014/025854 WO2014160117A1 (en) 2013-03-14 2014-03-13 Multiplex methylation-specific amplification systems and methods

Publications (1)

Publication Number Publication Date
CN105378107A true CN105378107A (zh) 2016-03-02

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CN201480027133.6A Pending CN105378107A (zh) 2013-03-14 2014-03-13 多重甲基化-特异性扩增系统和方法

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US (2) US9701999B2 (enExample)
EP (1) EP2971171A4 (enExample)
JP (2) JP2016512437A (enExample)
CN (1) CN105378107A (enExample)
WO (1) WO2014160117A1 (enExample)

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US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
EP3274440B1 (en) 2015-03-27 2026-03-04 Exact Sciences Corporation Detecting esophageal disorders
AU2016277943B2 (en) * 2015-06-15 2022-09-01 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
KR102811214B1 (ko) * 2015-10-30 2025-05-27 이그잭트 사이언시즈 코포레이션 복합 증폭 검출 분석 및 혈장으로부터 dna의 분리 및 검출 방법
CA3022911A1 (en) 2016-05-05 2017-11-09 Exact Sciences Development Company, Llc Detection of lung neoplasia by analysis of methylated dna
CN110267744A (zh) 2016-12-12 2019-09-20 塞弗德公司 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量
JP7289264B2 (ja) 2017-01-27 2023-06-09 エグザクト サイエンシーズ コーポレーション メチル化ｄｎａの分析による結腸腫瘍の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN114651070A (zh) * 2019-09-16 2022-06-21 精密科学公司 结构和温度依赖性瓣状核酸内切酶底物

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Also Published As

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US20140274735A1 (en) 2014-09-18
US9701999B2 (en) 2017-07-11
JP2016512437A (ja) 2016-04-28
US20170306395A1 (en) 2017-10-26
EP2971171A1 (en) 2016-01-20
EP2971171A4 (en) 2016-11-02
WO2014160117A1 (en) 2014-10-02
JP2019076100A (ja) 2019-05-23

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