CN105378096A - 寡糖组合物、糖蛋白以及在原核生物中产生它们的方法 - Google Patents

寡糖组合物、糖蛋白以及在原核生物中产生它们的方法 Download PDF

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Publication number
CN105378096A
CN105378096A CN201480028431.7A CN201480028431A CN105378096A CN 105378096 A CN105378096 A CN 105378096A CN 201480028431 A CN201480028431 A CN 201480028431A CN 105378096 A CN105378096 A CN 105378096A
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host cell
glcnac
protein
oligosaccharide composition
mannose
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Chinese (zh)
Inventor
亚当·C·费舍尔
朱迪思·H·梅利特
布莱恩·S·汉密尔顿
胡安·D·瓦尔德拉马-林科
马修·P·德里萨
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Glycobia Inc
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Glycobia Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0258—Escherichia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201480028431.7A 2013-03-14 2014-03-14 寡糖组合物、糖蛋白以及在原核生物中产生它们的方法 Pending CN105378096A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361785586P 2013-03-14 2013-03-14
US61/785,586 2013-03-14
PCT/US2014/026990 WO2014152137A2 (en) 2013-03-14 2014-03-14 Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes

Publications (1)

Publication Number Publication Date
CN105378096A true CN105378096A (zh) 2016-03-02

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CN201480028431.7A Pending CN105378096A (zh) 2013-03-14 2014-03-14 寡糖组合物、糖蛋白以及在原核生物中产生它们的方法

Country Status (8)

Country Link
US (2) US20140273163A1 (https=)
EP (1) EP2971030B8 (https=)
JP (1) JP2016518114A (https=)
KR (1) KR20160021075A (https=)
CN (1) CN105378096A (https=)
CA (1) CA2906671A1 (https=)
SG (2) SG11201508347YA (https=)
WO (1) WO2014152137A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110294810A (zh) * 2019-06-26 2019-10-01 中国疾病预防控制中心传染病预防控制所 一种含有人IgG1Fc和甘露聚糖结合凝集素C端的重组蛋白
WO2021227930A1 (zh) * 2020-05-14 2021-11-18 江南大学 一种提高重组n-乙酰葡糖胺转移酶ii可溶性表达的方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388372B (zh) * 2014-12-04 2017-07-21 江南大学 一种产软骨素的重组枯草芽孢杆菌及其应用
JP6762027B2 (ja) * 2016-08-25 2020-09-30 株式会社雅嘉貿易 シアロオリゴ糖の製造方法とその利用
BR112019015425A2 (pt) 2017-01-27 2020-05-26 University Of Florida Research Foundation, Incorporated Uma vacina de segurança alimentar para controlar salmonella enterica e reduzir campylobacter em aves domésticas
EP3844271A4 (en) 2018-08-29 2022-06-01 Council of Scientific and Industrial Research Recombinant microbial system for directed evolution of glycocins and a method of preparation thereof
CA3188909A1 (en) 2020-08-10 2022-02-17 Sofie AESAERT Production of an oligosaccharide mixture by a cell
ES2966260T3 (es) 2020-08-10 2024-04-19 Inbiose Nv Producción de una mezcla de oligosacáridos neutros no fucosilados por una célula
WO2022162704A1 (en) * 2021-02-01 2022-08-04 Dr. Reddy's Laboratories Limited Compositions comprising fusion protein and analytical attributes thereof
WO2023122641A1 (en) * 2021-12-22 2023-06-29 Zymergen Inc. Compositions and methods for producing acetic acid and polysaccharides
EP4265730A1 (en) * 2022-04-19 2023-10-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Cell-free enzymatic method for preparation of n-glycans
JP2025513499A (ja) 2022-04-19 2025-04-24 マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー Ｎ－グリカンの調製のための無細胞酵素法
WO2024218743A1 (en) * 2023-04-21 2024-10-24 Glycoera Ag Multi-functional molecules comprising glycans and uses thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020001831A1 (en) * 1998-11-18 2002-01-03 Defrees Shawn Low cost manufacture of oligosaccharides
US20040063911A1 (en) * 2001-10-10 2004-04-01 Neose Technologies, Inc. Protein remodeling methods and proteins/peptides produced by the methods
US20090311744A1 (en) * 2006-04-19 2009-12-17 Defrees Shawn Expression of o-glycosylated therapeutic proteins in prokaryotic microorganisms
CN101960017A (zh) * 2008-01-03 2011-01-26 康乃尔研究基金会有限公司 原核生物中的糖基化蛋白表达

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834251A (en) * 1994-12-30 1998-11-10 Alko Group Ltd. Methods of modifying carbohydrate moieties
DE19754622A1 (de) * 1997-12-09 1999-06-10 Antje Von Dr Schaewen Pflanzliche GntI-Sequenzen und Verwendung derselben zur Gewinnung von Pflanzen mit verminderter oder fehlender N-Acetylglucosaminyltransferase I (GnTI)-Aktivität
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
AU2002307037B2 (en) * 2001-04-02 2008-08-07 Biogen Idec Inc. Recombinant antibodies coexpressed with GnTIII
AU2003234445B2 (en) * 2002-05-02 2009-11-26 Vanderbilt University Mammalian genes involved in viral infection and tumor suppression
CA2591992A1 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins
CA2600391A1 (en) * 2005-03-07 2006-09-14 Plant Research International B.V. Glycoengineering in mushrooms
US20100143969A1 (en) * 2005-03-24 2010-06-10 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
WO2006112025A1 (ja) * 2005-04-15 2006-10-26 Japan Tobacco Inc. 新規なβ－ガラクトシド－α２，３－シアル酸転移酵素、それをコードする遺伝子およびその製造方法
US9927440B2 (en) * 2009-11-25 2018-03-27 Duke University Protein engineering
US9051593B2 (en) * 2009-12-21 2015-06-09 Trustees Of Dartmouth College Recombinant prokaryotes and use thereof for production of O-glycosylated proteins
WO2012050175A1 (ja) * 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 糖鎖の非還元末端がマンノース残基である糖蛋白質の製造方法
EP2643456B1 (en) * 2010-11-24 2016-05-11 Novartis International Pharmaceutical Ltd. Fusion enzymes having n-acetylglucosaminyltransferase activity
KR101906623B1 (ko) * 2011-03-04 2018-10-10 가부시키가이샤 도우사 고가쿠 겐큐쇼 시알산 함유 당쇄의 제조방법
EP2741772A1 (en) * 2011-08-08 2014-06-18 ETH Zurich Pasteurellaceae vaccines
WO2013067523A1 (en) * 2011-11-04 2013-05-10 Cornell University A prokaryote-based cell-free system for the synthesis of glycoproteins
RU2645252C2 (ru) * 2012-01-05 2018-02-19 Глюкос Финланд Ой Клетка нитчатых грибов с дефицитом протеаз и способы ее применения
WO2014048439A1 (en) * 2012-09-25 2014-04-03 Glycom A/S Glycoconjugate synthesis
ES2905107T3 (es) * 2012-10-12 2022-04-07 Glaxosmithkline Biologicals Sa Procedimientos de modificación de células hospedadoras

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020001831A1 (en) * 1998-11-18 2002-01-03 Defrees Shawn Low cost manufacture of oligosaccharides
US20040063911A1 (en) * 2001-10-10 2004-04-01 Neose Technologies, Inc. Protein remodeling methods and proteins/peptides produced by the methods
US20090311744A1 (en) * 2006-04-19 2009-12-17 Defrees Shawn Expression of o-glycosylated therapeutic proteins in prokaryotic microorganisms
CN101960017A (zh) * 2008-01-03 2011-01-26 康乃尔研究基金会有限公司 原核生物中的糖基化蛋白表达

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110294810A (zh) * 2019-06-26 2019-10-01 中国疾病预防控制中心传染病预防控制所 一种含有人IgG1Fc和甘露聚糖结合凝集素C端的重组蛋白
WO2021227930A1 (zh) * 2020-05-14 2021-11-18 江南大学 一种提高重组n-乙酰葡糖胺转移酶ii可溶性表达的方法

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US20160177355A1 (en) 2016-06-23
CA2906671A1 (en) 2014-09-25
JP2016518114A (ja) 2016-06-23
SG11201508347YA (en) 2015-11-27
EP2971030A2 (en) 2016-01-20
WO2014152137A8 (en) 2015-12-30
WO2014152137A3 (en) 2014-12-11
EP2971030B1 (en) 2018-10-17
US20140273163A1 (en) 2014-09-18
WO2014152137A2 (en) 2014-09-25
SG10201707565WA (en) 2017-10-30
EP2971030A4 (en) 2017-03-15
KR20160021075A (ko) 2016-02-24
EP2971030B8 (en) 2018-12-05

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Application publication date: 20160302