CN105363065A - Antibacterial and anti-inflammatory membrane - Google Patents
Antibacterial and anti-inflammatory membrane Download PDFInfo
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- CN105363065A CN105363065A CN201510773078.1A CN201510773078A CN105363065A CN 105363065 A CN105363065 A CN 105363065A CN 201510773078 A CN201510773078 A CN 201510773078A CN 105363065 A CN105363065 A CN 105363065A
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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Abstract
The invention discloses an antibacterial and anti-inflammatory membrane, which is mainly prepared from the following raw materials in parts by mass: 7-11 parts of fructus gardeniae, 6-10 parts of coix seeds, 11-15 parts of radix rubiae, 13-17 parts of rhizoma bletillae, 9-17 parts of concha arcae, 8-12 parts of dried orange peel, 7-10 parts of fistular onion stalk, 9-13 parts of herba violae, 6-12 parts of rhizoma cimicifugae, 13-16 parts of longstalk condorvine herb, 11-14 parts of cortex moutan, 40-60 parts of carboxymethyl chitosan, 10-20 parts of hyaluronic acid, 4-8 parts of lysozyme, 1-5 parts of polyvinyl alcohol, 1-4 parts of polyhexamethylene biguanidine hydrochloride and 2-5 parts of menthol. The membrane prepared by the invention has good absorptivity, and the membrane is well bonded to a wound surface and non-adhesive to wound; and the membrane is loaded with active ingredients of traditional Chinese medicines, so that the membrane, besides excellent antibacterial and anti-inflammatory effects, also has functions of stopping bleeding and removing stasis, cooling blood and relieving swelling, and the like; and the membrane is good in biocompatibility to the wound surface, conducive to wound healing and is capable of inhibiting hyperplastic scars.
Description
Technical field
The present invention relates to field of medical products, particularly relate to a kind of anti-inflammation thin film.
Background technology
In daily life and work, our skin often can the external world cause injury the factor (as surgical operation, external force, heat, electric current, chemical substance, low temperature) and body intrinsic factor (as local blood supply obstacle etc.) effect under suffer damage.Normal with the destruction of skin integrity and the loss of a certain amount of normal structure, meanwhile, the normal function of skin is impaired.After causing tissue to lack, local organization, by regeneration, reparation, reconstruction, is repaired.In the process of wound healing, there will be the hypertrophy of granulation tissue and the formation of scar tissue, and also have the existence of pathogenic microorganism in process, make skin occur infecting, wound surface is difficult to healing, once the bad meeting of wound healing causes larger injury to patient.
Publication No. CN103341203A(application number 201310253525.1) Chinese patent literature disclose the manufacture method of a kind of medical dressing, medical dressing manufacture method and dressing paste, this medical dressing comprises spirulina and extract thereof and Chinese herbal medicine, and wherein spirulina extract comprises phycocyanin, chlorophyll, superoxide dismutase, polysaccharide, ion and vitamin.Publication No. CN103690989A(application number 201310662052.0) Chinese patent literature disclose a kind of medical chitosan bleeding-stopping dressing, comprise the raw material of following weight portion: chitosan 25 ~ 40 parts, succinyl-chitosan 10 ~ 25 parts, hyaluronate sodium 5 ~ 15 parts, antibacterial 1 ~ 5 part, easing pain and diminishing inflammation agent 2 ~ 8 parts, starch 5 ~ 20 parts, plasticizer 2 ~ 10 parts.Publication No. CN104689365A(application number 201510118165.3) Chinese patent literature disclose compound bio dressing of a kind of short wound healing and preparation method thereof, this compound bio dressing is mixed by the poor medical gelatin of hydrophilic and the stronger hyaluronic acid of hydrophilic according to certain ratio and add cross-linking agent to make.
Existing Wound dressing respectively has pluses and minuses, and its performance still has much room for improvement, and therefore can develop one and have good antibacterial and anti-inflammation functions, the thin film of protecting from infection, to meet the needs of Present clinical treatment.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide simple, the degradable anti-inflammation thin film of a kind of technique.
Technical scheme of the present invention is:
A kind of anti-inflammation thin film, is prepared from primarily of following raw materials according, is mass parts: Fructus Gardeniae 7-11, Semen Coicis 6-10, Radix Rubiae 11-15, Pseudobulbus Bletillae (Rhizoma Bletillae) 13-17, Concha Arcae 9-17, Pericarpium Citri Reticulatae 8-12, Bulbus Allii Fistulosi 7-10, Herba Violae 9-13, Rhizoma Cimicifugae 6-12, Radix Marsdeniae Longipedis 13-16, Cortex Moutan 11-14, carboxymethyl chitosan 40-60, hyaluronic acid 10-20, lysozyme 4-8, polyvinyl alcohol 1-5, poly hexamethylene biguanide 1-4, Mentholum 2-5.
In preferred scheme, Fructus Gardeniae 8-10, Semen Coicis 7-9, Radix Rubiae 12-14, Pseudobulbus Bletillae (Rhizoma Bletillae) 14-16, Concha Arcae 11-15, Pericarpium Citri Reticulatae 9-11, Bulbus Allii Fistulosi 8-9, Herba Violae 10-12, Rhizoma Cimicifugae 8-10, Radix Marsdeniae Longipedis 14-15, Cortex Moutan 12-13, carboxymethyl chitosan 45-55, hyaluronic acid 12-18, lysozyme 5-7, polyvinyl alcohol 2-4, poly hexamethylene biguanide 2-3, Mentholum 3-4.
Be more preferably, Fructus Gardeniae 9, Semen Coicis 8, Radix Rubiae 13, Pseudobulbus Bletillae (Rhizoma Bletillae) 15, Concha Arcae 13, Pericarpium Citri Reticulatae 10, Bulbus Allii Fistulosi 8.5, Herba Violae 11, Rhizoma Cimicifugae 9, Radix Marsdeniae Longipedis 14.5, Cortex Moutan 12.5, carboxymethyl chitosan 50, hyaluronic acid 15, lysozyme 6, polyvinyl alcohol 3, poly hexamethylene biguanide 2.5, Mentholum 3.5.
The preparation method of described a kind of anti-inflammation thin film, step is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, after Steam by water bath, take out, dry;
(2) Concha Arcae washing water of rice is flooded, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa saline with boiling, little fire boils 10-16 minute (preferred, little fire boils 13 minutes), and take out, sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 20-28 minute (preferably, heating boils 24 minutes), then add decocting in water, big fire is boiled rear little fire and is boiled, filter, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, big fire is boiled rear little fire and is boiled 25 minutes, filter, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill, film forming, sterilizing, obtains thin film.
The preparation method of described a kind of anti-inflammation thin film, step (1) Steam by water bath 12-18 minute (preferred, Steam by water bath 14-16 minute; Be more preferably, Steam by water bath 15 minutes).
The preparation method of described a kind of anti-inflammation thin film, step (2) washing water of rice dipping 2-3 hour (preferred, to flood 2.5 hours with washing water of rice).
The preparation method of described a kind of anti-inflammation thin film, the concentration of step (3) saline is that 13%-21%(is preferred, and the concentration of saline is 15%-19%; Be more preferably, the concentration of saline is 17%).
The preparation method of described a kind of anti-inflammation thin film, step (4) adds in decocting in water again, and the 3-5 that amount of water is boiled raw materials quality is (preferred, 4 times that amount of water is boiled raw materials quality) doubly.
The preparation method of described a kind of anti-inflammation thin film, step (4) big fire is boiled rear little fire and is boiled 28-40 minute (preferred, little fire boils 32-36 minute; Be more preferably, little fire boils 34 minutes).
The preparation method of described a kind of anti-inflammation thin film, step (6) 20 DEG C (preferably, leaves standstill 5-7 hour in standing 4-8 hour; Be more preferably, leave standstill 6 hours).
A kind of anti-inflammation method for manufacturing thin film provided by the invention is simple, non-stimulated to skin.In addition, excellent results of the present invention also shows:
1, absorbability of the present invention is good, is combined well, adhesion wound with wound surface;
2, institute of the present invention made membrane load has Effective Component of Chinese Medicine, except having good antibacterial and anti-inflammation functions, and the effects such as the blood stasis dispelling that stops blooding in addition, removing heat from blood detumescence;
3, the present invention and wound surface good biocompatibility, be beneficial to wound healing, suppresses scar hyperplasia.
Detailed description of the invention
Describe technical scheme of the present invention in detail below in conjunction with embodiment and experimental example, but protection domain is not limited thereto.
embodiment 1a kind of anti-inflammation thin film, is prepared from (every part of 10g) primarily of following raw materials according: Fructus Gardeniae 7 parts, Semen Coicis 6 parts, 11 parts, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae) 13 parts, Concha Arcae 9 parts, Pericarpium Citri Reticulatae 8 parts, Bulbus Allii Fistulosi 7 parts, Herba Violae 9 parts, Rhizoma Cimicifugae 6 parts, Radix Marsdeniae Longipedis 13 parts, Cortex Moutan 11 parts, carboxymethyl chitosan 40 parts, hyaluronic acid 10 parts, lysozyme 4 parts, polyvinyl alcohol 1 part, poly hexamethylene biguanide 1 part, Mentholum 2 parts.
The preparation method of above-mentioned a kind of anti-inflammation thin film is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, Steam by water bath, after 12 minutes, takes out, dries;
(2) Concha Arcae washing water of rice is flooded 2 hours, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa concentration be the saline of 13% with boiling, little fire boils 10 minutes, takes out, and sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 20 minutes, add the decocting in water of boiled raw materials quality 3 times again, big fire is boiled rear little fire and is boiled 28 minutes, filters, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, and big fire is boiled rear little fire and boiled 25 minutes, filters, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill 4 hours, film forming, sterilizing, obtains thin film.
embodiment 2a kind of anti-inflammation thin film, is prepared from (every part of 10g) primarily of following raw materials according: Fructus Gardeniae 11 parts, Semen Coicis 10 parts, 15 parts, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae) 17 parts, Concha Arcae 17 parts, Pericarpium Citri Reticulatae 12 parts, Bulbus Allii Fistulosi 10 parts, Herba Violae 13 parts, Rhizoma Cimicifugae 12 parts, Radix Marsdeniae Longipedis 16 parts, Cortex Moutan 14 parts, carboxymethyl chitosan 60 parts, hyaluronic acid 20 parts, lysozyme 8 parts, polyvinyl alcohol 5 parts, poly hexamethylene biguanide 4 parts, Mentholum 5 parts.
A kind of preparation method of anti-inflammation thin film is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, Steam by water bath, after 18 minutes, takes out, dries;
(2) Concha Arcae washing water of rice is flooded 3 hours, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa concentration be the saline of 21% with boiling, little fire boils 16 minutes, takes out, and sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 28 minutes, add the decocting in water of boiled raw materials quality 5 times again, big fire is boiled rear little fire and is boiled 40 minutes, filters, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, and big fire is boiled rear little fire and boiled 25 minutes, filters, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill 8 hours, film forming, sterilizing, obtains thin film.
Typical case: xxx, female, 46 years old.Main suit: shank is because of carelessness by scalds of motorcycle exhaust pipe, shallow two degree of scalds, adopt hydrogen peroxide cleaning wound surface, alcohol disinfecting surrounding skin, MEIBAO is used to be applied on wound surface, and it is also fixing on wound surface with a kind of anti-inflammation plastic film covering of the present embodiment gained, within every 6 hours, change dressings once, thin film does not change, wound surface incrustation two days later, without sepage oozing of blood, wound surface is clean, pain is not obvious, after four days, skin has granulation tissue to grow, after one week, wound reduces in week, there is partial necrosis tissue loss, after 10 days, wound surface is dry, all be coated with newborn epithelial tissue, thinner, after 12 days, wound surface heals completely, without clear scar.
embodiment 3a kind of anti-inflammation thin film, is prepared from (every part of 10g) primarily of following raw materials according: Fructus Gardeniae 8 parts, Semen Coicis 7 parts, 12 parts, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae) 14 parts, Concha Arcae 11 parts, Pericarpium Citri Reticulatae 9 parts, Bulbus Allii Fistulosi 8 parts, Herba Violae 10 parts, Rhizoma Cimicifugae 8 parts, Radix Marsdeniae Longipedis 14 parts, Cortex Moutan 12 parts, carboxymethyl chitosan 45 parts, hyaluronic acid 12 parts, lysozyme 5 parts, polyvinyl alcohol 2 parts, poly hexamethylene biguanide 2 parts, Mentholum 3 parts.
A kind of preparation method of anti-inflammation thin film is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, Steam by water bath, after 14 minutes, takes out, dries;
(2) Concha Arcae washing water of rice is flooded 2 hours, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa concentration be the saline of 15% with boiling, little fire boils 10 minutes, takes out, and sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 20 minutes, add the decocting in water of boiled raw materials quality 3 times again, big fire is boiled rear little fire and is boiled 32 minutes, filters, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, and big fire is boiled rear little fire and boiled 25 minutes, filters, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill 5 hours, film forming, sterilizing, obtains thin film.
Typical case: xxx, man, 25 years old.When riding electric motor car working accidentally and private car collide, knocked down on ground and towed several meters, wound is rubbed in many places with it, after carrying out local skin sterilization, with povidone iodine wiping wound surface, and with a kind of anti-inflammation plastic film covering of the present embodiment gained on wound surface, change dressings every day once, thin film does not change, after 1 day, wound surface has a small amount of sepage to ooze out, wound surface has mild redness, thin film and wound surface are without adhesion, after 2 days, wound surface is without sepage, totally, incrustation is had to generate, redness disappears substantially, without infecting, the visible newborn epithelial tissue in wound surface edge after 5 days, after 8 days, wound surface incrustation comes off, wound healing, cicatrix is not obvious.
embodiment 4a kind of anti-inflammation thin film, is prepared from (every part of 10g) primarily of following raw materials according: Fructus Gardeniae 10 parts, Semen Coicis 9 parts, 14 parts, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae) 16 parts, Concha Arcae 15 parts, Pericarpium Citri Reticulatae 11 parts, Bulbus Allii Fistulosi 9 parts, Herba Violae 12 parts, Rhizoma Cimicifugae 10 parts, Radix Marsdeniae Longipedis 15 parts, Cortex Moutan 13 parts, carboxymethyl chitosan 55 parts, hyaluronic acid 18 parts, lysozyme 7 parts, polyvinyl alcohol 4 parts, poly hexamethylene biguanide 3 parts, Mentholum 4 parts.
A kind of preparation method of anti-inflammation thin film is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, Steam by water bath, after 16 minutes, takes out, dries;
(2) Concha Arcae washing water of rice is flooded 3 hours, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa concentration be the saline of 19% with boiling, little fire boils 16 minutes, takes out, and sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 28 minutes, add the decocting in water of boiled raw materials quality 5 times again, big fire is boiled rear little fire and is boiled 36 minutes, filters, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, and big fire is boiled rear little fire and boiled 25 minutes, filters, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill 7 hours, film forming, sterilizing, obtains thin film.
embodiment 5a kind of anti-inflammation thin film, is prepared from (every part of 10g) primarily of following raw materials according: Fructus Gardeniae 9, Semen Coicis 8 parts, 13 parts, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae) 15 parts, Concha Arcae 13 parts, Pericarpium Citri Reticulatae 10 parts, Bulbus Allii Fistulosi 8.5 parts, Herba Violae 11 parts, Rhizoma Cimicifugae 9 parts, Radix Marsdeniae Longipedis 14.5 parts, Cortex Moutan 12.5 parts, carboxymethyl chitosan 50 parts, hyaluronic acid 15 parts, lysozyme 6 parts, polyvinyl alcohol 3 parts, poly hexamethylene biguanide 2.5 parts, Mentholum 3.5 parts.
A kind of preparation method of anti-inflammation thin film is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, Steam by water bath, after 15 minutes, takes out, dries;
(2) Concha Arcae washing water of rice is flooded 2.5 hours, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa concentration be the saline of 17% with boiling, little fire boils 13 minutes, takes out, and sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 24 minutes, add as the decocting in water of boiled raw materials quality 4 times again, big fire is boiled rear little fire and is boiled 34 minutes, filters, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, and big fire is boiled rear little fire and boiled 25 minutes, filters, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan, hyaluronic acid and polyvinyl alcohol respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill 6 hours, film forming, sterilizing, obtains thin film.
Pharmacology and the application of the present invention's primary raw material used are as follows:
Fructus Gardeniae: base is former: be the fruit of Maguireothamnus speciosus Fructus Gardeniae.Nature and flavor: bitter, cold.Gui Jing: GUIXIN, lung, tri-jiao channel.Function cures mainly: fruit: pathogenic fire purging relieving restlessness, clearing away heat and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body.Vexed for calentura, jaundice dark coloured urine, blood strangury and dry pain, heat in blood tells nosebleed, conjunctival congestion and swelling pain, pathogenic fire,toxin and furuncles; External treatment bruise pain.
Semen Coicis: base is former: be the kernel of grass Semen Coicis.Nature and flavor: sweet, light, cool.Gui Jing: return spleen, stomach, lung meridian.Function cures mainly: spleen invigorating eliminating dampness by diuresis, eliminating impediment antidiarrheal, and row is dense in heat clearing away.For edema, beriberi, dysuria, arthralgia chiefly caused by damp pathogen contracture, diarrhea due to hypofunction of the spleen, lung abscess, intestinal carbuncle; Verruca plana.
Radix Rubiae: base is former: be the root welding technology of Maguireothamnus speciosus Radix Rubiae.Nature and flavor: bitter, cold.Gui Jing: return Liver Channel.Function cures mainly: removing heat from blood, and hemostasis, blood stasis dispelling, stimulates the menstrual flow.For spitting blood, epistaxis, metrorrhagia, traumatic hemorrhage, amenorrhea stasis blocking, arthralgia, tumbling and swelling.
Pseudobulbus Bletillae (Rhizoma Bletillae): base is former: be the tuber of orchid Pseudobulbus Bletillae (Rhizoma Bletillae).Nature and flavor: bitter, sweet, puckery, are slightly cold.Gui Jing: return lung, liver, stomach warp.Function cures mainly: astringing to arrest bleeding, detumescence and promoting granulation.Spit blood for hemoptysis, traumatic hemorrhage, sore swollen toxin, chapped skin; Pulmonary tuberculosis hemoptysis, ulcer haemorrhage.
Concha Arcae: base is former: be the shell of Carnis Arca inflata section animal Scapharca broughtonii, Tegillarca granosa or Scapharca subcrenata.Nature and flavor: salty are flat.Gui Jing: return lung, stomach, Liver Channel.Function cures mainly: expectorant blood stasis dispelling, hard masses softening and resolving, relieving gastric hyperacidity to alleviate stomachache.For the long-pending knot of pertinacious phlegm, sticky difficulty is coughed up, goiter, scrofula, lump in the abdomen mass in the abdomen, stomachache pantothenic acid.
Pericarpium Citri Reticulatae: base is former: be the dry mature skin of rutaceae orange and variety thereof.Nature and flavor: bitter, pungent, temperature.Gui Jing: return lung, spleen channel.Function cures mainly: regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm.For fullness in the epigastrium and chest, lack of appetite and vomiting, cough with copious phlegm.
Bulbus Allii Fistulosi: base is former: be the bulb of liliaceous plant Herba Alii fistulosi.Nature and flavor: pungent, temperature.Gui Jing: enter lung, stomach warp.Function cures mainly: relieving the exterior syndrome by diaphoresis, activates yang, diuresis.For headache due to common cold, nasal obstruction; Dysuria is controlled in external, carbuncle furuncle and phyma poison.
Herba Violae: base is former: be the herb of Violaceae Chinese violet.Nature and flavor: bitter, acrid, cold.Gui Jing: GUIXIN, Liver Channel.Function cures mainly: heat-clearing and toxic substances removing, and removing heat from blood is subsided a swelling.For furuncle swelling toxin, carbuncle carbuncle on the back, erysipelas, venom.
Rhizoma Cimicifugae: base is former: be the root stock of ranunculaceae plant Rhizoma Cimicifugae, Cimicifuga Dahurica and C.heracleifolia.Nature and flavor: pungent, micro-sweet, are slightly cold.Gui Jing: return lung, spleen, stomach, large intestine channel.Function cures mainly: deliver rash, heat-clearing and toxic substances removing, elevate a turnable ladder yang-energy.For headache due to pathogenic wind-heat, toothache, aphtha, laryngopharynx swelling and pain, measles without adequate eruption, YANG toxin syndrome sends out speckle; Proctoptosis, uterine prolapse.
Radix Marsdeniae Longipedis: base is former: be the Herb of asclepiadaceae plant Radix Marsdeniae Longipedis.Nature and flavor: sweet, micro-hardship, puckery, temperature.Poisonous.Function cures mainly: promoting blood circulation and hemostasis, relieving cough and asthma.For traumatic injury, rheumatalgia, internal hemorrhage, bronchitis, asthma; Fracture is controlled in external, traumatic hemorrhage.
Cortex Moutan: base is former: be the root bark of ranunculaceae peony.Nature and flavor: bitter, pungent, are slightly cold.Gui Jing: GUIXIN, liver, kidney channel.Function cures mainly: clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling.For maculae caused by violent heat pathogen, hematemesis and epistaxis, night fever abating at dawn, lossless hectic fever due to YIN-deficiency, amenorrhea dysmenorrhea, carbuncle sore tumefacting virus, falls and flutters the pain of injury.
Carboxymethyl chitosan: a kind of water-solubility chitosan derivative, safety non-toxic also has antibacterial, antibacterial, stable emulsifying effect, non-immunogenicity, biodegradable, the excellent compatibility with biological tissue, is easily absorbed by body, also can promote the reparation of bone, promoting growth of cell, arrhythmia, anticoagulant active.Important purposes is had in daily use chemicals, food, papermaking, pharmacy etc.
Hyaluronic acid: the large-scale polysaccharide that basic structure is made up of two dissacharide units D-Glucose aldehydic acid and N-acetyl-glucosamine.It can improve skin-nourishing metabolism, and make that skin is tender, smooth, wrinkle removing, increase elasticity, prevent aging, be again good Percutaneous absorption enhancer while moisturizing.With other nutritional labelings with the use of, can play and promote the better effect of alimentation.Can be used for treatment of arthritis and accelerating wound healing.Use it in cosmetics, can play unique protection skin effect, skin moisturizing can be kept smooth, fine and smooth tender, high resilience, has the effect of wrinkle resistant, crease-resistant, aesthetic health care and recovery skin physiology function.
Lysozyme: also known as muramidase or N-acetylmuramide lycanohydrlase is a kind of alkaline enzyme that can be hydrolyzed mucopolysaccharide in pathogenic bacterium.White or micro white lyophilized powder, can be used as a kind of natural infection material with bactericidal action.There are antibacterial, antiviral, hemostasis, reducing swelling and alleviating pain and accelerate the effects such as organized renewing function.
Polyvinyl alcohol: off-white powder, for the manufacture of Pioloform, polyvinyl acetal, resistance to gasoline pipeline and vinylon synthetic fibers, fabric-treating agent, emulsifying agent, paper coating, binding agent, glue etc.Pharmaceutical grade Polyethylene alcohol, be different from chemical industry rank polyvinyl alcohol, it is a kind of extremely safe macromolecule organic, to human non-toxic, have no side effect, there is good biocompatibility, especially in medical treatment as its aqueous gel being widely used in ophthalmology, wound dressing and artificial joint, simultaneously at polyvinyl alcohol film at medicinal film, also there is use the aspects such as artificial kidney film.
Poly hexamethylene biguanide: be a kind of high molecular polymer, there is the ability of extremely strong kill bacteria and virus, the bacteriostasis time is long, to the corrosion-free effect of various metal material, degradable, does not pollute environment, and toxicity is low, the features such as use safety, are widely used in fields such as medicine, culture fishery, leather processing, work domestics use of water.Can be used for the aspects such as textile industry, paper-making industry, wood-processing industry, rubber industry, agricultural, also can as the flocculating agent of sewage disposal.
Mentholum: molecular formula C
10h
20o, being pharmaceutically used as excitants, acting on skin or mucosa, having refrigerant itching-relieving action.
test example 1a kind of anti-inflammation thin film (embodiment 5) of gained of the present invention is to union of wounded skin effect and bacteriostatic activity:
1, to union of wounded skin Experiment on Function object: new zealand white rabbit.Test method: rabbit back is lost hair or feathers, at the other 2cm place, both sides of spinal column, excision full thickness skin is organized to superficial fascia, cuts out the circular wound surface that a diameter is about 1.5cm respectively, by prepare staphylococcus aureus, escherichia coli and Pseudomonas aeruginosa suspension (1 × 10
9cFU/mL) drop in 0.5ml on wound surface with syringe, after 10 minutes, be attached to wound surface with common medical sponge dressing (matched group) and the made film dressing of the present invention (experimental group), binder is fixed, and raises separately.Respectively at postoperative 1 day, 3 days, 5 days, 10 days, 15 days, within 20 days, observe wound surface sepage and ooze out situation, healing and healing time.
2, bacteriostatic activity test strain: staphylococcus aureus (ATCC25923) and escherichia coli (ATCC25922).
Test method: it is 1 × 10 that strain culture dilution is mixed with concentration
8~ 5 × 10
8the bacterial suspension of CFU/mL is as inoculation liquid, the inoculation liquid of staphylococcus aureus and the Candida albicans prepared is dipped by swab stick, spread upon on nutrient agar panel, uniform application 3 this, institute's made membrane of the present invention and common medical sponge dressing are cut into the disk that diameter is 10mm, be placed on scribble bacterium liquid flat board on, observe the size of inhibition zone cultivate 24h at 37 DEG C after.
Result of the test: postoperative 1 day of (1) wound healing, experimental group wound has a small amount of sepage to ooze out, the visible a small amount of purulent secretion of matched group, postoperative 3 days, two groups of wounds all have contraction, and matched group wound surface has sepage, and part wound surface has crust to be formed, experimental group sepage disappearance wound surface is dry, and all have crust to generate, and 5 days after operation, two groups of wounds all obviously shrink, experimental group wound surface has a large amount of granulation to grow, and matched group wound surface edge has granulation to grow.Postoperative 10 days, experimental group wound surface had newborn epithelial tissue to cover, and matched group edge of wound has newborn epithelial tissue, postoperative 12 days, experimental group wound healed substantially, and matched group part heals, 15 days after operation, two groups of wounds all heal, and experimental group wound granulation is solid, healed surface compared with normal skin is lower slightly, cicatrix is thin and soft, becomes wire, matched group cicatrix compared with normal cutaneous protuberance, and wider comparatively hard, comparatively matched group is good for visible experimental group wound surface healing state.
(2) bacteriostatic activity institute of the present invention made membrane is respectively 11.7mm and 13.2mm to staphylococcus aureus and colibacillary inhibition zone size, common medical sponge dressing is respectively 2.7mm and 4.2mm to staphylococcus aureus and colibacillary inhibition zone size, and the bacteriostatic activity of visible institute of the present invention made membrane is better than common medical sponge dressing.
In sum, the made thin film of the present invention has antibacterial and wound healing effect.
Chinese crude drug Fructus Gardeniae used in the present invention, Semen Coicis, Radix Rubiae, Pseudobulbus Bletillae (Rhizoma Bletillae), Concha Arcae, Pericarpium Citri Reticulatae, Bulbus Allii Fistulosi, Herba Violae, Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and Cortex Moutan can be worked in coordination with and be played effect such as hemostasis blood stasis dispelling, removing heat from blood detumescence etc., prepare degradable, the good biological dressing of biocompatibility in conjunction with carboxymethyl chitosan and hyaluronic acid.
It should be pointed out that detailed description of the invention is the more representational example of the present invention, obvious technical scheme of the present invention is not limited to above-described embodiment, can also have a lot of distortion.Those of ordinary skill in the art, with the present invention clear and definite disclosed or according to undoubted the obtaining of the written description of file, the claimed scope of this patent all should be thought.
Claims (10)
1. an anti-inflammation thin film, is characterized in that, is prepared from primarily of following raw materials according, is mass parts: Fructus Gardeniae 7-11, Semen Coicis 6-10, Radix Rubiae 11-15, Pseudobulbus Bletillae (Rhizoma Bletillae) 13-17, Concha Arcae 9-17, Pericarpium Citri Reticulatae 8-12, Bulbus Allii Fistulosi 7-10, Herba Violae 9-13, Rhizoma Cimicifugae 6-12, Radix Marsdeniae Longipedis 13-16, Cortex Moutan 11-14, carboxymethyl chitosan 40-60, hyaluronic acid 10-20, lysozyme 4-8, polyvinyl alcohol 1-5, poly hexamethylene biguanide 1-4, Mentholum 2-5.
2. a kind of anti-inflammation thin film as claimed in claim 1, is characterized in that, Fructus Gardeniae 8-10, Semen Coicis 7-9, Radix Rubiae 12-14, Pseudobulbus Bletillae (Rhizoma Bletillae) 14-16, Concha Arcae 11-15, Pericarpium Citri Reticulatae 9-11, Bulbus Allii Fistulosi 8-9, Herba Violae 10-12, Rhizoma Cimicifugae 8-10, Radix Marsdeniae Longipedis 14-15, Cortex Moutan 12-13, carboxymethyl chitosan 45-55, hyaluronic acid 12-18, lysozyme 5-7, polyvinyl alcohol 2-4, poly hexamethylene biguanide 2-3, Mentholum 3-4.
3. a kind of anti-inflammation thin film as claimed in claim 1, is characterized in that, Fructus Gardeniae 9, Semen Coicis 8, Radix Rubiae 13, Pseudobulbus Bletillae (Rhizoma Bletillae) 15, Concha Arcae 13, Pericarpium Citri Reticulatae 10, Bulbus Allii Fistulosi 8.5, Herba Violae 11, Rhizoma Cimicifugae 9, Radix Marsdeniae Longipedis 14.5, Cortex Moutan 12.5, carboxymethyl chitosan 50, hyaluronic acid 15, lysozyme 6, polyvinyl alcohol 3, poly hexamethylene biguanide 2.5, Mentholum 3.5.
4. the preparation method of a kind of anti-inflammation thin film as described in as arbitrary in claim 1-3, it is characterized in that, step is:
(1) Radix Rubiae and Cortex Moutan are placed in hot water leaching 30 minutes, take out, section or shredding, then Fructus Gardeniae and Herba Violae are pulverized, together be placed in frying pan by four kinds of raw materials, intense fire is heated to coke black and takes out, and mixes thoroughly, be placed in steamer with Sucus Zingberis and hydromel, after Steam by water bath, take out, dry;
(2) Concha Arcae washing water of rice is flooded, take out and be placed in heating in crucible, be calcined to and be popular in, take out, pulverize after cooling completely;
(3) by Pericarpium Citri Reticulatae and Bulbus Allii Fistulosi and Oryza glutinosa saline with boiling, little fire boils 10-16 minute (preferred, little fire boils 13 minutes), and take out, sieve removes Oryza glutinosa, dries;
(4) Semen Coicis, Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cimicifugae, Radix Marsdeniae Longipedis and step (1) (2) (3) made raw material are placed in non iron container, add Chinese liquor and do not have raw material, heating boils 20-28 minute (preferably, heating boils 24 minutes), then add decocting in water, big fire is boiled rear little fire and is boiled, filter, collect filtrate, filtering residue adds water and did not have raw material 2cm to boil again, big fire is boiled rear little fire and is boiled 25 minutes, filter, merge twice filtrate, be concentrated into 1/5 of cumulative volume, centrifugal remove impurity, obtained concentrated solution;
(5) prepare carboxymethyl chitosan solution, hyaluronic acid solution and poly-vinyl alcohol solution respectively with distilled water, Mentholum dissolve with ethanol is mixed with solution;
(6) carboxymethyl chitosan solution is mixed with Mentholum solution and step (4) made concentrated solution, add lysozyme and poly hexamethylene biguanide stirring, add hyaluronic acid solution to be again uniformly mixed, finally add poly-vinyl alcohol solution mixing, add triethanolamine solution and regulate pH to 7.0, evacuation and centrifugal degassing, in casting mold, 20 DEG C leave standstill, film forming, sterilizing, obtains thin film.
5. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, step (1) Steam by water bath 12-18 minute (preferred, Steam by water bath 14-16 minute; Be more preferably, Steam by water bath 15 minutes).
6. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, step (2) washing water of rice dipping 2-3 hour (preferred, to flood 2.5 hours with washing water of rice).
7. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, the concentration of step (3) saline is that 13%-21%(is preferred, and the concentration of saline is 15%-19%; Be more preferably, the concentration of saline is 17%).
8. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, step (4) adds in decocting in water again, and the 3-5 that amount of water is boiled raw materials quality is (preferred, 4 times that amount of water is boiled raw materials quality) doubly.
9. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, step (4) big fire is boiled rear little fire and boiled 28-40 minute (preferred, little fire boils 32-36 minute; Be more preferably, little fire boils 34 minutes).
10. the preparation method of a kind of anti-inflammation thin film as claimed in claim 4, is characterized in that, step (6) 20 DEG C (preferably, leaves standstill 5-7 hour in standing 4-8 hour; Be more preferably, leave standstill 6 hours).
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| CN201510773078.1A CN105363065A (en) | 2015-11-13 | 2015-11-13 | Antibacterial and anti-inflammatory membrane |
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| CN201510773078.1A CN105363065A (en) | 2015-11-13 | 2015-11-13 | Antibacterial and anti-inflammatory membrane |
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| CN112336909A (en) * | 2020-08-25 | 2021-02-09 | 江苏奥普莱医疗用品有限公司 | Medical collagen dressing and preparation method thereof |
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