CN105341530A - Clean type special compound feed for stichopus japonicus and processing method of clean type special compound feed - Google Patents

Clean type special compound feed for stichopus japonicus and processing method of clean type special compound feed Download PDF

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Publication number
CN105341530A
CN105341530A CN201510847878.3A CN201510847878A CN105341530A CN 105341530 A CN105341530 A CN 105341530A CN 201510847878 A CN201510847878 A CN 201510847878A CN 105341530 A CN105341530 A CN 105341530A
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enzymolysis
parts
beans
meal
clean type
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黄炳山
宋志东
李培玉
王际英
张利民
王世信
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Shandong Marine Resource and Environment Research Institute
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Shandong Marine Resource and Environment Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention relates to a clean type special compound feed for stichopus japonicus and a processing method of the clean type special compound feed, and belongs to the technical field of processing of aquaculture feeds. The clean type special compound feed is prepared from the following raw materials in parts by weight: 10-40 parts of dried marine algae powder, 3-80 parts of a reaction mixture of enzymolysis soybean meal and enzymolysis alfalfa powder, 5-15 parts of microcrystalline cellulose, 3-5 parts of fish meal, 3-5 parts of wheat flour, 2-5 parts of yeast powder, 4-7 parts of starch, 4-8 parts of sodium alginate, 20-30 parts of sea mud, 0.7-2 parts of complex vitamins and 1-3 parts of composite mineral substances. The clean type special compound feed is prepared through the following technological steps of (1) material preparation; (2) crushing; (3) secondary mixing; (4) extrusion and granulation; (5) drying; and (6) cooling and packaging. According to the clean type special compound feed and the processing method thereof disclosed by the invention, biological enzymolysis treatment is performed on plant protein raw materials by utilizing compound protease, and then plant protein zymolyte and the dried marine algae powder are in an appropriate compounding ratio, so that the whole nutrient value of the feed is increased, and the digestibility and the absorptivity of the feed are improved. According to a making technology, a corpuscule technique in nutrition loss dynamics is utilized, so that the pollution-free easily-clean type special high-efficiency compound feed for the stichopus japonicus is prepared.

Description

A kind of clean type stichopus japonicus special compound feed and processing method
Technical field
The present invention relates to a kind of clean type stichopus japonicus special compound feed and processing method, be specifically adopt nutrition slow-release combine with technique to add enzymolysis of plants protein production clean type stichopus japonicus special compound feed, belong to aquaculture feed processing technique field.
Background technology
In recent years, apostichopus japonicus culture started to enter intensive high-density breeding, consumes the marine algaes such as sargassum thunbergii, sargassum, sea-tangle in a large number, both caused the shortage of marine algae resource, and turn improved the price of marine alga in cultivation.Although adopt at present terrestrial plant albumen to substitute algae powder alleviate pressure to algae powder to a certain extent, usually cause stichopus japonicus grazing rate to reduce, grow the situations such as slack-off after substituting, terrestrial plant substitutes algae powder and can only maintain in lower ratio.Trace it to its cause, compare with fish and shrimp, the digestion zymogram of stichopus japonicus self is comparatively simple, and stichopus japonicus digests and assimilates low shortcoming by adopting to ingest in a large number to take in more bait to make up, and simple substituting then causes its abilities of digestive and absorption to reduce further.In addition, in apostichopus japonicus Selenka feed, adopt mash feed, powder-material scatters and disappears large in water, and especially some soluble nutrients are very easily lost in water, relatively reduce stichopus japonicus and effectively absorb it more.Meanwhile, the nutritional labeling of scattering and disappearing usually causes the bottom, pond portion severe contamination, is bonded at the mixed feed not easy to clean bottom pond, more increases the weight of bottom and pollutes, and causes stichopus japonicus significantly " avlidance behavior ", increases the death of stichopus japonicus, bring loss to apostichopus japonicus culture.Although sheet feed stuff has certain anti-property of scattering and disappearing, but it is different from mash feed, sheet feed stuff often sinks to bottom one pocket, owing to lacking strong attractant material in feed formula, the sense organ of stichopus japonicus is difficult to the position detecting feed at short notice, so also relatively add the holdup time of sheet feed stuff in water, it is the increase in fact scattering and disappearing of nutriment.One of effective method addressed these problems, it is exactly the lost dynamic law for the various nutriments in formula, design a kind of stiff stability mixed feed containing enriching Attractant, make its disintegration layer by layer in water, slow trace release Attractant, lure stichopus japonicus initiatively to ingest, need to have property material easy to digest simultaneously in mixed feed, auxiliary raising stichopus japonicus is to the digestion of nutriment.
Summary of the invention
The object of the invention is to solve the shortcoming that stichopus japonicus is low to the constitutive material digestibility in feed, absorb available nutrient deficiency, a kind of clean type stichopus japonicus special efficient mixed feed and processing method are provided, to improve the overall nutritive value of feed, simultaneously in conjunction with slowly-releasing food calling technology, improve stichopus japonicus initiatively ingestion ability, promote that it grows.
The present invention is by a kind of simple material treatment process efficiently, compound protease is utilized to carry out biological enzymolysis process to plant protein material, again vegetable protein enzymolysis thing and seawood meal are carried out suitable proportioning, thus the overall nutritive value of fortified feed, improve the absorbability easy to digest of feed.In processing, utilize nutrition in the early stage dynamics research that scatters and disappears to set up particulate technology, form a kind of pollution-free easy clean type stichopus japonicus special efficient mixed feed.
The present invention is achieved through the following technical solutions:
A kind of clean type stichopus japonicus special compound feed, its special feature is to be obtained by following raw material components, is respectively by the weight portion of each component: seawood meal 10-40 part, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 3-80 part, microcrystalline cellulose 5-15 part, fish meal 3-5 part, wheat flour 3-5 part, dusty yeast 1-5 part, starch 4-7 part, sodium alginate 4-8 part, ooze 20-30 part, B B-complex 0.7-2 part, composite mineral matter 1-3 part.
The preparation method of described enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture is:
1) complex enzyme reactant liquor is prepared:
With total consumption of dregs of beans and alfalfa meal for reference to substrate, get alkali protease, non-starch polysaccharide enzyme and food flavor enzyme, taken amount is respectively according to 0.08 ~ 0.22%, 0.1 ~ 0.4%, 0.06 ~ 0.3% with dregs of beans and alfalfa meal gross weight ratio, allotment mixing, after the stirring that adds water makes it fully dissolve, put into the container with agitating function and temp. control function, be warming up to 45 ~ 55 DEG C;
2) enzymolysis dregs of beans and enzymolysis alfalfa meal:
Dregs of beans and Alfalfa are carried out ultramicro grinding, cross 120 ~ 150 eye mesh screens, add in the complex enzyme reactant liquor prepared in proportion, adding proportion with bean cake powder and alfalfa meal weight/complex enzyme reactant liquor volume for 1:5 ~ 1:2 (w/v, g/mL), put into enzyme digestion reaction tank and carry out enzyme digestion reaction, speed of agitator is set to 30-60r/min, the enzymolysis time is make enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture after 4-6 hour, 4-6 hour.
In enzymolysis process, i.e. in the preparation method of described enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture, the weight ratio of dregs of beans and alfalfa meal requires as 2:1 ~ 3:1.
Described alkali protease is commercially available prod, requires activity >=50000U/g.
Described non-starch polysaccharide enzyme is commercially available aquatic products special product, requires containing zytase >=8000U/g, 1,4 beta-glucanase >=2000U/g, 'beta '-mannase >=150U/g, cellulase >=300U/g.
Described food flavor enzyme is commercially available prod, requires activity >=30000U/g.
Preferably, described clean type stichopus japonicus special compound feed, preferentially selects and is obtained by following raw material components, be respectively by the weight portion of each component: seawood meal 30 parts, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 55 parts, microcrystalline cellulose 10 parts, fish meal 4 parts, wheat flour 4 parts, dusty yeast 3 parts, starch 5 parts, sodium alginate 5 parts, ooze 25 parts, B B-complex 1 part, composite mineral matter 2 parts;
In this enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture, dregs of beans and alfalfa meal part by weight are 3:1, and bean cake powder and alfalfa meal weight/complex enzyme reactant liquor volume are 1:5 (g/mL).
The processing method of above-mentioned clean type stichopus japonicus special compound feed, comprises the following steps:
1) get the raw materials ready: take various raw material by the weight portion of each component;
2) pulverize: by step 1) in load weighted seawood meal, microcrystalline cellulose, fish meal, wheat flour, dusty yeast, starch, sodium alginate and ooze carry out first time and mix, incorporation time is 60-120 second, carries out meal after mixing, and meal fineness is passed through screen cloth; Then adopt micronizer to carry out second time to pulverize, more than smashing fineness to 120 order, standard screen oversize is less than 5%.
3) secondary mixing: by step 1) load weighted B B-complex and composite mineral matter add in the material after separating twice, the enzymolysis dregs of beans and enzymolysis alfalfa meal reactant liquor that make is added again according to proportioning, enzymolysis dregs of beans and the mixing of enzymolysis alfalfa meal reactant liquor must be ensured before adding, carry out second time mix and blend, incorporation time is 90-120 second.
4) extruding is granulated: granulation pore size of template is granulation length requirement is 0.6-1.0 times of grain diameter.
5) dry: bake out temperature controls at 60-70 DEG C, and the material moisture after oven dry must not more than 12%.
6) cooler cooling and packaging: temperature of charge is cooled in the scope not higher than ambient temperature 8 DEG C, carries out finished product packing.
The present invention is by a kind of simple material treatment process efficiently, compound protease is utilized to carry out biological enzymolysis process to plant protein material, again vegetable protein enzymolysis thing and seawood meal are carried out suitable proportioning, thus the overall nutritive value of fortified feed, improve the absorbability easy to digest of feed.In processing, utilize nutrition in the early stage dynamics research that scatters and disappears to set up particulate technology, form a kind of pollution-free easy clean type stichopus japonicus special efficient mixed feed.Itself and prior art contrast has following characteristics:
1. rationally have employed enzymolysis dregs of beans and enzymolysis alfalfa meal in formula, both reduced the ratio of seawood meal, its plant polypeptide produced enters in stichopus japonicus body can very fast being absorbed and used, and improves the nutritive value of whole formula.
2. Appropriate application sodium alginate is as main binder, and adopts ooze as the carrier of soluble nutrients, thus makes feed produce slow release effect, disintegration releasing nutrients material layer by layer, play the effect of lasting food calling, stichopus japonicus is produced and initiatively concentrates effect of ingesting, form good feeding habits.
3. adopted microparticle processing technology is based upon nutrition and scatters and disappears on the basis of dynamic (dynamical) system research, and compared with sheet feed stuff, after its disintegration, area coverage is larger, and its nutrition preservation effect is far above powder-material.In a word, this particulate material avoids the use drawback of sheet and powdery, collects the advantage such as characteristic easy to digest and nutrition keeping quality.
4. present invention obviates the freeze drying process to enzyme digestion reaction liquid, by scientific algorithm enzyme digestion reaction liquid proportioning, directly enzyme digestion reaction liquid prepared burden, high degree reduce cost, save and use water.
The applicable object of feed of the present invention is the stichopus japonicus of monomer weight more than 10 grams, and compared with the stichopus japonicus special feed of conventional formulation manufacture, attractant is better, and absorption more easy to digest, grows faster.
Detailed description of the invention
Below provide the specific embodiment of the present invention, be used for being further described formation of the present invention.
Embodiment 1
The clean type stichopus japonicus special compound feed of the present embodiment, selects and is obtained by following raw material components, be respectively by the weight portion of each component: seawood meal 30 parts, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 55 parts, microcrystalline cellulose 10 parts, fish meal 4 parts, wheat flour 4 parts, dusty yeast 3 parts, starch 5 parts, sodium alginate 5 parts, ooze 25 parts, B B-complex 1 part, composite mineral matter 2 parts.
Wherein the preparation method of enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture is:
1) complex enzyme reactant liquor is prepared: alkali protease is (commercially available, activity is 50000U/g), (commercially available aquatic products is special for non-starch polysaccharide enzyme, containing zytase 8000U/g, 1,4 beta-glucanase 2000U/g, 'beta '-mannase 150U/g, cellulase 300U/g) and food flavor enzyme (commercially available, active 30000U/g) add up to 0.1% of weight ratio according to dregs of beans and alfalfa meal, the ratio of 0.2% and 0.2% prepares (use amount first should estimating dregs of beans and alfalfa meal according to consumption), the stirring that adds water makes it fully dissolve, put into the container with agitating function and temp. control function, be warming up to 50 DEG C.
2) enzymolysis dregs of beans and enzymolysis alfalfa meal: dregs of beans and Alfalfa (by weight 2:1) are carried out ultramicro grinding, cross 120 ~ 150 eye mesh screens, according to 1:5 (w/v, g/mL) ratio adds in the complex enzyme reactant liquor prepared, enzyme digestion reaction is carried out in reaction vessel, speed of agitator is set to 30r/min, and the enzymolysis time is 6 hours, makes enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture after 6 hours.
The processing method of clean type stichopus japonicus special compound feed in the present embodiment:
1) get the raw materials ready: take various raw material by the weight portion of each component;
2) pulverize: by step 1) in load weighted seawood meal, microcrystalline cellulose, fish meal, wheat flour, dusty yeast, starch, sodium alginate and ooze carry out first time and mix, incorporation time is 60 seconds, carries out meal after mixing, and meal fineness is passed through screen cloth; Then adopt micronizer to carry out second time to pulverize, more than smashing fineness to 120 order, standard screen oversize is less than 5%.
3) secondary mixing: by step 1) load weighted B B-complex and composite mineral matter add in the material after separating twice, the enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture that make is added again according to proportioning, enzymolysis dregs of beans and the mixing of enzymolysis alfalfa meal reaction mixture must be ensured before adding, carry out second time mix and blend, incorporation time is 100 seconds.
4) extruding is granulated: granulation pore size of template is granulation length requirement is 0.8 times of grain diameter.
5) dry: bake out temperature controls at 60 DEG C, and the material moisture after oven dry must not more than 12%.
6) cooler cooling and packaging: temperature of charge is cooled in the scope not higher than ambient temperature 8 DEG C, carries out finished product packing.
Embodiment 2
The clean type stichopus japonicus special compound feed of the present embodiment, adopts following raw material components to obtain, is respectively by the weight portion of each component: seawood meal 20 parts, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 50 parts, microcrystalline cellulose 15 parts, fish meal 5 parts, wheat flour 3 parts, dusty yeast 2 parts, starch 4 parts, sodium alginate 6 parts, ooze 28.3 parts, B B-complex 0.7 part, composite mineral matter 1 part.
Wherein the preparation method of enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture is:
1) complex enzyme reactant liquor is prepared: alkali protease is (commercially available, active 80000U/g), (commercially available aquatic products is special for non-starch polysaccharide enzyme, containing zytase 10000U/g, 1,4 beta-glucanase 4000U/g, 'beta '-mannase 180U/g, cellulase 500U/g) and food flavor enzyme (commercially available, active 50000U/g) add up to weight ratio to be 0.15% according to dregs of beans and alfalfa meal respectively, the ratio of 0.08% and 0.1% prepares, the stirring that adds water makes it fully dissolve, put into the container with agitating function and temp. control function, be warming up to 55 DEG C;
2) enzymolysis dregs of beans and enzymolysis alfalfa meal: dregs of beans and Alfalfa are carried out ultramicro grinding for 3:1 mixes by weight proportion, cross 120 eye mesh screens, according to 3:10 (w/v, g/mL) ratio adds the complex enzyme reactant liquor prepared, enzyme digestion reaction is carried out in reaction vessel, speed of agitator is set to 30r/min, and the enzymolysis time is 5 hours, makes enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture after 5 hours.
The processing method of clean type stichopus japonicus special compound feed in the present embodiment:
1) get the raw materials ready: take various raw material by the weight portion of each component;
2) pulverize: by step 1) in load weighted seawood meal, microcrystalline cellulose, fish meal, wheat flour, dusty yeast, starch, sodium alginate and ooze carry out first time and mix, incorporation time is 120 seconds, carries out meal after mixing, and meal fineness is passed through screen cloth; Then adopt micronizer to carry out second time to pulverize, more than smashing fineness to 120 order, standard screen oversize is less than 5%.
3) secondary mixing: by step 1) load weighted B B-complex and composite mineral matter add in the material after separating twice, the enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture that make is added again according to proportioning, enzymolysis dregs of beans and the mixing of enzymolysis alfalfa meal reaction mixture must be ensured before adding, carry out second time mix and blend, incorporation time is 120 seconds.
4) extruding is granulated: granulation pore size of template is granulation length requirement is 0.8 times of grain diameter.
5) dry: bake out temperature controls at 65 DEG C, and the material moisture after oven dry must not more than 12%.
6) cooler cooling and packaging: temperature of charge is cooled in the scope not higher than ambient temperature 8 DEG C, carries out finished product packing.
Embodiment 3
The clean type stichopus japonicus special compound feed of the present embodiment, is obtained by following raw material components, is respectively by the weight portion of each component: seawood meal 40 parts, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 42.5part, microcrystalline cellulose 5 parts, fish meal 3 parts, wheat flour 3 parts, dusty yeast 2 parts, starch 4 parts, sodium alginate 4 parts, ooze 20 parts, B B-complex 1 part, composite mineral matter 1 part.
Wherein the preparation method of enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture is:
1) complex enzyme reactant liquor is prepared: by alkali protease, non-starch polysaccharide enzyme and food flavor enzyme respectively according to the weight ratio 0.1% with dregs of beans and alfalfa meal, the ratio of 0.2% and 0.2% prepares, the stirring that adds water makes it fully dissolve, put into the container with agitating function and temp. control function, be warming up to 50 DEG C.Wherein alkali protease 60000U/g; Non-starch polysaccharide enzyme is containing zytase 10000U/g, 1,4 beta-glucanase 2000U/g, 'beta '-mannase 200U/g, cellulase 300U/g; Food flavor enzyme 30000U/g.
2) enzymolysis dregs of beans and enzymolysis alfalfa meal: dregs of beans and Alfalfa (by weight 2:1) are carried out ultramicro grinding, cross 150 eye mesh screens, according to 2:5 (w/v, g/mL) ratio adds in the complex enzyme reactant liquor prepared, enzyme digestion reaction is carried out in reaction vessel, speed of agitator is set to 40r/min, and the enzymolysis time is 6 hours, makes enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture after 6 hours.
The processing method of clean type stichopus japonicus special compound feed in the present embodiment:
1) get the raw materials ready: take various raw material by the weight portion of each component;
2) pulverize: by step 1) in load weighted seawood meal, microcrystalline cellulose, fish meal, wheat flour, dusty yeast, starch, sodium alginate and ooze carry out first time and mix, incorporation time is 120 seconds, carries out meal after mixing, and meal fineness is passed through screen cloth; Then adopt micronizer to carry out second time to pulverize, more than smashing fineness to 120 order, standard screen oversize is less than 5%.
3) secondary mixing: by step 1) load weighted B B-complex and composite mineral matter add in the material after separating twice, the enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture that make is added again according to proportioning, enzymolysis dregs of beans and the mixing of enzymolysis alfalfa meal reaction mixture must be ensured before adding, carry out second time mix and blend, incorporation time is 110 seconds.
4) extruding is granulated: granulation pore size of template is granulation length requirement is identical with grain diameter.
5) dry: bake out temperature controls at 65 DEG C, and the material moisture after oven dry must not more than 12%.
6) cooler cooling and packaging: temperature of charge is cooled in the scope not higher than ambient temperature 8 DEG C, carries out finished product packing.
For showing actual effect of the present invention, spy provides the instantiation adopting clean type stichopus japonicus compound feed culturing stichopus japonicus of the present invention:
1.2014 on May 3, in, stichopus japonicus 480 bull that monomer weighs about 20 grams is bought from From Shandong Rizhao in certain cultivation base, adopts closed circulating water system cultivation, is divided into 2 groups, often organize 3 ponds, two groups a kind of commodity mash feed of omnidistance use and a kind of feed of the present invention respectively, on July 2nd, 2014, in 2 months periods, feed is fabulous to stichopus japonicus attractant, feeding behaviour rule, stichopus japonicus growth rapidly, the many and formed stools of ight soil amount.The sea cucumber rate of body weight gain of feed of the present invention of feeding reaches 100-120%, survival rate about 93%, and the sea cucumber rate of body weight gain of full algae powder feed group of feeding is only 50-70%, survival rate about 80%, the culture efficiency of feed to stichopus japonicus adding a certain amount of enzymolysis of plants albumen has exceeded full algae powder feed.
Contrast discovery by experiment, almost can't see stichopus japonicus bottom full algae mash feed and get over vestige, ight soil is less, illustrates that stichopus japonicus ingests poor, and sticky end is serious, easily rots at the smelly end, is difficult to clean.And feed of the present invention is relative to common powdery commodity material, there is clean not polluted water, non-sticky end, attractant is good, promote to concentrate and ingest, improve the advantage of the speed of growth, the low-carbon (LC) of stichopus japonicus, environmental protection, innoxious cultivation can be realized.
2. Peng Lai plant, cultivation substance on April 10th, 2014 30 grams of stichopus japonicus 50,000 tails, cement pit is utilized to cultivate, wherein 30,000 anal spines join formula forage of the present invention of feeding, another 20,000 anal spines join seawood meal of feeding, the conditions such as breeding density, water temperature, quantity of exchanged water are identical, feed 6 months, i.e. October 10 the same year, the stichopus japonicus of feed of the present invention fed all increases weight and reaches about 190%, and the stichopus japonicus that seawood meal is fed all increases weight, and is about 150%, and greatly reduces and change water frequency, odorless water phenomenon occurs, and conclusion shows feed product advantage of the present invention and gives prominence to.
Listed embodiment is only the present invention's preferably embodiment above, and protection scope of the present invention is not limited to above given embodiment, and every all technical schemes achieved by thinking of the present invention, all belong to protection scope of the present invention.

Claims (8)

1. a clean type stichopus japonicus special compound feed, is characterized in that being obtained by following raw material components, is respectively by the weight portion of each component: seawood meal 10-40 part, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 3-80 part, microcrystalline cellulose 5-15 part, fish meal 3-5 part, wheat flour 3-5 part, dusty yeast 1-5 part, starch 4-7 part, sodium alginate 4-8 part, ooze 20-30 part, B B-complex 0.7-2 part, composite mineral matter 1-3 part.
2., according to clean type stichopus japonicus special compound feed a kind of described in claim 1, it is characterized in that the preparation method of described enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture is:
1) complex enzyme reactant liquor is prepared:
With total consumption of dregs of beans and alfalfa meal for reference to substrate, get alkali protease, non-starch polysaccharide enzyme and food flavor enzyme, taken amount is respectively according to 0.08 ~ 0.22%, 0.1 ~ 0.4%, 0.06 ~ 0.3% with dregs of beans and alfalfa meal gross weight ratio, allotment mixing, after the stirring that adds water makes it fully dissolve, put into the container with agitating function and temp. control function, be warming up to 45 ~ 55 DEG C;
2) enzymolysis dregs of beans and enzymolysis alfalfa meal:
Dregs of beans and Alfalfa are carried out ultramicro grinding, cross 120 ~ 150 eye mesh screens, add in the complex enzyme reactant liquor prepared in proportion, adding proportion with bean cake powder and alfalfa meal weight/complex enzyme reactant liquor volume for 1:5 ~ 1:2 (w/v, g/mL), put into enzyme digestion reaction tank and carry out enzyme digestion reaction, speed of agitator is set to 30-60r/min, the enzymolysis time is make enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture after 4-6 hour, 4-6 hour.
3., according to clean type stichopus japonicus special compound feed a kind of described in claim 2, it is characterized in that the weight ratio of dregs of beans and alfalfa meal in the preparation method of described enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture requires as 2:1 ~ 3:1.
4., according to clean type stichopus japonicus special compound feed a kind of described in claim 2, it is characterized in that described alkali protease is commercially available prod, require activity >=50000U/g.
5. according to clean type stichopus japonicus special compound feed a kind of described in claim 2, it is characterized in that described non-starch polysaccharide enzyme is commercially available aquatic products special product, require containing zytase >=8000U/g, 1,4 beta-glucanase >=2000U/g, 'beta '-mannase >=150U/g, cellulase >=300U/g.
6., according to clean type stichopus japonicus special compound feed a kind of described in claim 2, it is characterized in that described food flavor enzyme is commercially available prod, require activity >=30000U/g.
7. according to clean type stichopus japonicus special compound feed a kind of described in claim 1 or 2, it is characterized in that selecting and obtained by following raw material components, be respectively by the weight portion of each component: seawood meal 30 parts, enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture 55 parts, microcrystalline cellulose 10 parts, fish meal 4 parts, wheat flour 4 parts, dusty yeast 3 parts, starch 5 parts, sodium alginate 5 parts, ooze 25 parts, B B-complex 1 part, composite mineral matter 2 parts;
In its enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture, dregs of beans and alfalfa meal part by weight are 3:1, and bean cake powder and alfalfa meal weight/complex enzyme reactant liquor volume are 1:5 (g/mL).
8., according to clean type stichopus japonicus special compound feed a kind of described in claim 1 or 2, it is characterized in that the processing method of clean type stichopus japonicus special compound feed comprises the following steps:
1) get the raw materials ready: take various raw material by the weight portion of each component;
2) pulverize: by step 1) in load weighted seawood meal, microcrystalline cellulose, fish meal, wheat flour, dusty yeast, starch, sodium alginate and ooze carry out first time and mix, incorporation time is 60-120 second, carries out meal after mixing, and meal fineness is passed through screen cloth; Then adopt micronizer to carry out second time to pulverize, more than smashing fineness to 120 order, standard screen oversize is less than 5%;
3) secondary mixing: by step 1) load weighted B B-complex and composite mineral matter add in the material after separating twice, the enzymolysis dregs of beans and enzymolysis alfalfa meal reaction mixture that make is added again according to proportioning, enzymolysis dregs of beans and the mixing of enzymolysis alfalfa meal reaction mixture must be ensured before adding, carry out second time mix and blend, incorporation time is 90-120 second;
4) extruding is granulated: granulation pore size of template is granulation length requirement is 0.6-1.0 times of grain diameter;
5) dry: bake out temperature controls at 60-70 DEG C, and the material moisture after oven dry must not more than 12%;
6) cooler cooling and packaging: temperature of charge is cooled in the scope not higher than ambient temperature 8 DEG C, carries out finished product packing.
CN201510847878.3A 2015-11-27 2015-11-27 Clean type special compound feed for stichopus japonicus and processing method of clean type special compound feed Pending CN105341530A (en)

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CN106666247A (en) * 2017-03-23 2017-05-17 威海金牌生物科技股份有限公司 Foraging feed of special compound feed for breeding stichopus japonicus and preparation method of foraging feed
CN106819617A (en) * 2016-12-05 2017-06-13 浙江海洋大学 A kind of rheum officinale fish meal of growth promotion diseases prevention and its application
CN109619269A (en) * 2018-11-21 2019-04-16 浙江海洋大学 Special feed for sea cucumber cultured in net cage
CN111466500A (en) * 2020-05-09 2020-07-31 山东省海洋资源与环境研究院 Low-fish-meal turbot feed and preparation method thereof
CN111728104A (en) * 2020-07-24 2020-10-02 山东省海洋资源与环境研究院 Micro-ecological type special feed for stichopus japonicus and preparation method

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CN106666224A (en) * 2016-12-05 2017-05-17 浙江海洋大学 Marine fish health-care feed and application thereof
CN106819617A (en) * 2016-12-05 2017-06-13 浙江海洋大学 A kind of rheum officinale fish meal of growth promotion diseases prevention and its application
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CN109619269A (en) * 2018-11-21 2019-04-16 浙江海洋大学 Special feed for sea cucumber cultured in net cage
CN111466500A (en) * 2020-05-09 2020-07-31 山东省海洋资源与环境研究院 Low-fish-meal turbot feed and preparation method thereof
CN111728104A (en) * 2020-07-24 2020-10-02 山东省海洋资源与环境研究院 Micro-ecological type special feed for stichopus japonicus and preparation method

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Application publication date: 20160224