CN105325459A

CN105325459A - Preparation method of rice germ inhibitor coming from xylose

Info

Publication number: CN105325459A
Application number: CN201510928822.0A
Authority: CN
Inventors: 王忠文; 杨平; 浦相君; 时婷婷; 曾东强; 张君成
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2015-12-14
Filing date: 2015-12-14
Publication date: 2016-02-17

Abstract

The invention discloses a preparation method of rice germ inhibitor coming from xylose. The method includes the following steps of firstly, preparing compound xylose into a xylose solution; secondly, heating the xylose solution in a high-pressure and high-temperature heating kettle to 126 DEG C, and keeping the temperature for 30 minutes; thirdly, taking the xylose solution out of the heating kettle, naturally cooling the xylose solution at a room temperature, and forming the rice germ inhibitor; fourthly, conducting antibacterial effect detection on the inhibitor, wherein the obtained inhibitor is prepared into a culture medium, ustilaginoidea virens is planted in and cultured under the appropriate temperature conditions, and it can be seen that the growth and development of ustilaginoidea virens can be strongly restrained through the product obtained by conducting high-temperature treatment on xylose. The method has the advantages that through the simple high-temperature treatment technology, restraining substances for restraining the growth and development of ustilaginoidea virens efficiently can be obtained from compound xylose.

Description

A kind of preparation method coming from the rice false smut mortifier of wood sugar

Technical field

The present invention relates to plant pathology to learn a skill and Pesticide Science technology.Specifically a kind of preparation method coming from the rice false smut mortifier of wood sugar.

Technical background

Infect by rice false smut (Ustilaginoideavirens (Cooke) Takahashi) the false smut whole world caused to occur, belong to the Major Diseases of China's Rice Production, in some rice districts, false smut even exceedes rice blast and becomes the great disease of Rice Production.Be limited to and enough understanding lacked to the plague law of plant disease, current and from now on quite over a long time in, the control of plant disease still depends on medicine (agricultural chemicals) control, and the control of false smut is also like this.Therefore, the research and development of false smut protective agents just become the important technology work solving false smut problem.

In history, false smut one is directly subordinate to lighter Minor diseases of causing harm, and just the fast development of nearly more than ten years, just rises to the great disease of Rice Production.Owing to failing to obtain enough attention in history, the related basic study of false smut control is still weak, and the medicament research and development for false smut control is delayed especially.In fact, be limited to the biological shallow understanding of rice false smut, current medicament research and development of preventing and treating false smut is almost confined to directly using of market agricultural chemicals or existing medicine composite usage, and the fermentation culture method of the technology of preparing of current agriculture the effective elements of the medicine mainly chemical synthesis process and microbial pesticide.Inventor's latest find wood sugar itself is harmless to rice false smut, has found the rice false smut mortifier deriving from wood sugar, growing of this mortifier strong inhibition rice false smut simultaneously, has potentiality and the application prospect of exploitation.But the acquisition of this mortifier is not by chemosynthesis approach, neither biofermentation ways for training be passed through, but be obtained by high temperature treatment approach.Inventor finds that this high-temperature processing method is simply efficient, there is great difference with the preparation method of existing protective agents.

Summary of the invention

The object of the invention is to provide a kind of preparation method coming from the rice false smut mortifier of wood sugar.

The technical scheme that the present invention solves the problems of the technologies described above is as follows:

Coming from a preparation method for the rice false smut mortifier of wood sugar, is that the compound wood sugar harmless to false smut is carried out high temperature treatment, and to have grown inhibiting inhibiting substances until generate to rice false smut, the step of preparation method is as follows:

1. solution preparation:

20g wood sugar is dissolved in 1000ml water, is made into xylose solution.

2. high temperature treatment:

Xylose solution high-voltage high-temperature steam step 1 be made into is heated to 126 DEG C and maintains 30 minutes.

3. cool:

Stop the heating operation of step 2, after slow steam discharge decompression, take out the xylose solution in autoclave, after room temperature cools naturally, namely become the inhibiting substances that rice false smut can be suppressed to grow.

4. the inspection of bacteriostasis:

Cooled for step 3 Xylose is made medium, and implant rice false smut, cultivate under appropriate temperature conditions, growing of visible rice false smut is strongly inhibited.Obviously, to the harmless wood sugar of rice false smut after step 1 to the technical finesse of step 3, decompose and generate inhibiting substances rice false smut being played to strong inhibition effect.

Advantage of the present invention

The present invention utilizes high temperature treatment wood sugar to obtain the efficient inhibiting substances suppressing rice false smut to grow, and technology is simple.

Accompanying drawing explanation

Fig. 1 is that the present invention contrasts CK to rice false smut suppression power test figure.

Fig. 2 is that the efficient mortifier of the present invention suppresses power test figure to rice false smut.

Embodiment

Below in conjunction with accompanying drawing and embodiment, the invention will be further described.

Inventor finds in false smut research, and compound wood sugar itself is harmless to rice false smut, and on xylose media, false smut energy normal growth is grown, as shown in Figure 1; But high temperature treatment can cause the change of the biological effectiveness generation matter of wood sugar, originally harmless wood sugar is after high temperature treatment, the normal growth of energy strong inhibition rice false smut is grown, with the xylose media that high temperature treatment is crossed, cultivate rice false smut, growing of rice false smut is subject to obvious suppression, as shown in Figure 2.Result has not only found a kind of mortifier suppressing rice false smut to grow, also form a kind of preparation method preparing rice false smut inhibiting substances, namely by high temperature treatment technology, the efficient inhibiting substances suppressing rice false smut growth can be obtained from simplification compound wood sugar.

Embodiment

Coming from a preparation method for the rice false smut mortifier of wood sugar, is that xylose solution is carried out high temperature treatment, operates as follows:

1. solution preparation:

20g wood sugar is dissolved in 1000ml water, is made into xylose solution.

2. high temperature treatment:

3. cool:

4. the inspection of bacteriostasis: cooled for step 3 xylose solution is made medium, and concrete operations are as follows:

First prepare potato agar basal medium, proportioning is: potato 200g, agar 40g, water 1000ml, conventional high temperature sterilizing; Then this basal medium and the cooled xylose solution of step 3 are carried out mixed in equal amounts, forming proportioning is: the mortifier medium of potato 100g, wood sugar 10g, agar 20g, distilled water 1000ml, becomes mortifier culture plate after being down flat plate.

Contrast CK is, xylose solution filtration sterilization step 1 is made into biofilter, then by the xylose solution of this filtration sterilization, with the potato agar basal medium of above-mentioned preparation, mixed in equal amounts is carried out under 60 DEG C of states, form same proportioning: the CK medium of potato 100g, wood sugar 10g, agar 20g, distilled water 1000ml, after being down flat plate, become contrast CK culture plate.The difference of contrast CK and aforementioned mortifier medium is, the wood sugar of contrast CK is without high temperature treatment.

Culture plate implants rice false smut, and every culture plate implants 9 spore liquid, is placed in 28 DEG C of thermostatic and cultivates, take out observation after 5 days.

On contrast CK (wood sugar without high temperature treatment) culture plate, grow 9 rice false smut bacterium colonies clearly, as shown in Figure 1.

And on mortifier culture plate, 9 spores are implanted point and are showed no growth sign, as shown in Figure 2.Obviously, the xylose solution through 126 DEG C of high temperature treatment creates the inhibiting substances suppressing rice false smut growth.

Utilize technology of the present invention, in the safe range of firing equipment, the temperature of high temperature treatment is higher, the duration of high temperature treatment is longer, the suppression usefulness of wood sugar high temperature treatment product is higher.

Claims

1. come from a preparation method for the rice false smut mortifier of wood sugar, it is characterized in that, compound wood sugar is carried out high temperature treatment, to have grown inhibiting inhibiting substances until generate to rice false smut, the step of method is as follows:

1) xylose solution preparation:

20g wood sugar is dissolved in 1000ml water, is made into xylose solution;

2) high temperature treatment:

By step 1) the xylose solution high-voltage high-temperature steam that is made into is heated to 126 DEG C and maintains 30 minutes;

3) cool:

Stop step 2) heating operation, and slowly after steam discharge decompression, take out the xylose solution in autoclave, after room temperature cools naturally, namely become the inhibiting substances that rice false smut can be suppressed to grow;

4) inspection of bacteriostasis:

By step 3) cooled Xylose makes medium, and implant rice false smut, cultivate under appropriate temperature conditions, growing of visible rice false smut is strongly inhibited; Obviously, to the harmless wood sugar of rice false smut through step 1) to step 3) technical finesse after, decompose and generate inhibiting substances rice false smut being played to strong inhibition effect.