CN105309478A - Ustilaginoidea virens inhibitor from xylose - Google Patents
Ustilaginoidea virens inhibitor from xylose Download PDFInfo
- Publication number
- CN105309478A CN105309478A CN201510928942.0A CN201510928942A CN105309478A CN 105309478 A CN105309478 A CN 105309478A CN 201510928942 A CN201510928942 A CN 201510928942A CN 105309478 A CN105309478 A CN 105309478A
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- CN
- China
- Prior art keywords
- false smut
- xylose
- wood sugar
- mortifier
- rice
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses an ustilaginoidea virens inhibitor from xylose. The ustilaginoidea virens inhibitor is from a xylose compound and is generated by carrying out high-temperature treatment on xylose. The ustilaginoidea virens inhibitor from xylose has the beneficial effects that the ustilaginoidea virens inhibitor from xylose has a strong effect of inhibiting growth and development of ustilaginoidea virens; the ustilaginoidea virens inhibitor from xylose can provide efficient and effective technical beginning and an efficient and effective technological approach for research and development of ustilaginoidea virens pesticide.
Description
Technical field
The present invention relates to plant pathology to learn a skill and Pesticide Science technology.Specifically a kind of rice false smut mortifier coming from wood sugar.
Technical background
Infect by rice false smut (Ustilaginoideavirens (Cooke) Takahashi) the false smut whole world caused to occur, belong to the Major Diseases of China's Rice Production, in some rice districts, false smut even exceedes rice blast and becomes the great disease of Rice Production.Be limited to and enough understanding lacked to the plague law of plant disease, current and from now on quite over a long time in, the control of plant disease still depends on medicine (agricultural chemicals) control, and the control of false smut is also like this.Therefore, the research and development of false smut protective agents just become the important technology work solving false smut problem.
In history, false smut one is directly subordinate to lighter Minor diseases of causing harm, and just the fast development of nearly more than ten years, just rises to the great disease of Rice Production.Owing to failing to obtain enough attention in history, the related basic study of false smut control is still weak, and the medicament research and development for false smut control is delayed especially.In fact, be limited to the biological shallow understanding of rice false smut, current medicament research and development of preventing and treating false smut is almost confined to directly using of market agricultural chemicals or existing medicine composite usage, and the research and development of the source medicine of relevant false smut control are very rarely seen.In view of false smut is to the significant impact of rice grain safety, the protective agents research and development strengthening false smut are obviously very necessary.Technically, fully excavate and find the inhibiting substances that rice false smut grows, be not only conducive to the lead drug finding to prevent and treat false smut, also will widen the approach of false smut protective agents field research and development.
Summary of the invention
The present invention seeks to, a kind of rice false smut mortifier coming from wood sugar is provided.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
The rice false smut mortifier deriving from wood sugar derives from a compound wood sugar, is generated after high temperature treatment by wood sugar.This mortifier has effect that strong inhibition rice false smut grows.This mortifier can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Benefit effect of the present invention:
A kind of rice false smut mortifier deriving from wood sugar of the present invention can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Accompanying drawing explanation
Fig. 1 is that the present invention contrasts CK to rice false smut suppression power test figure.
Fig. 2 is that the efficient mortifier of the present invention suppresses power test figure to rice false smut.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the invention will be further described.
Inventor finds in false smut research for many years, in the conventional medium containing wood sugar (1%), the various plants pathogen energy normal growths such as Pyricularia oryzae are grown, but rice false smut can not be grown by normal growth, is once once thinking that compound wood sugar can suppress growing of rice false smut by mistake.Up-to-date test finds, be conducive to rice false smut growth potato agar be in the medium of background, add wood sugar (content is 1%), when the wood sugar added is not through high temperature treatment, rice false smut can be grown by normal growth on this medium, as shown in Figure 1; And when same wood sugar is through high temperature treatment, rice false smut but can not be grown by normal growth on this medium, as shown in Figure 2.Obviously, compound wood sugar itself is harmless to rice false smut, but the biological effectiveness of the high temperature treatment product of wood sugar there occurs the change of matter, become and suppress the normal growth of rice false smut to be grown, namely wood sugar is after high temperature treatment, create material rice false smut being played to strong inhibition effect, such result of study yet there are no open precedent.This inhibiting substances is to the strong bacteriostasis efficacy of rice false smut, there is potentiality and the application prospect of exploitation, on the one hand, the protective agents of this mortifier as false smut directly can be researched and developed, or verify the structure of this mortifier and as guide's material, develop similar and the stronger agent of inhibitory action; And on the other hand, by studying this mortifier to the action target of rice false smut and the mechanism of action, and then excavate and explore the more effective agent for these targets; Thus the mortifier deriving from compound wood sugar disclosed by the invention, can provide effective and feasible technology beginning and technological approaches for the research and development of false smut protective agents.
Embodiment
In the potato agar medium background that suitable rice false smut grows, add the rice false smut mortifier deriving from wood sugar, can detect that the inhibiting substances deriving from wood sugar is to the bacteriostasis of rice false smut easily.Concrete implementation and operation is as follows:
1. prepare the prewired medium P of potato agar
The potato agar medium adopting suitable rice false smut to grow makes background medium, and the prewired ratio of background medium is: potato 200g, agar 40g, distilled water 1000ml.The prewired medium P of potato agar is obtained after conventional high temperature sterilizing.
2. prepare xylose solution A and high temperature treatment product B thereof
In proportion: wood sugar 20g, distilled water 1000ml, be made into xylose solution, at normal temperatures with biofilter by this xylose solution filtration sterilization, obtain without high temperature treatment but be in the xylose solution A of sterilizing state.This xylose solution A is placed in ordinary high pressure high-temperature sterilization pot, 126 DEG C of high temperature treatment 30 minutes, after cooling, obtains the high temperature treatment product B of wood sugar.
3. detect the bacteriostasis of mortifier
Under aseptic condition, prewired for potato agar medium P is carried out mixed in equal amounts with the high temperature treatment product B of xylose solution A, wood sugar respectively, change into culture plate, the identical 2 kinds of medium of formation background: the high temperature treatment product free medium of A xylose media, B wood sugar, and the proportioning of these 2 kinds of medium the same (potato 100g, wood sugar 10g, agar 20g, water 1000ml).
Rice false smut is implanted into culture plate, and every dull and stereotyped implantation spore liquid 9 point, is then placed in 28 DEG C of constant incubators and cultivates, take out observation after 5 days.
On A xylose media, grow 9 rice false smut bacterium colonies clearly, as shown in Figure 1, obviously on this medium, rice false smut energy normal growth is grown.
And on the high temperature treatment product free medium of B wood sugar, 9 spores are implanted point and are showed no any growth sign, as shown in Figure 2, obviously this medium efficiently suppresses growing of rice false smut.Very clear and definite, there is the mortifier of rice false smut in this medium, this mortifier efficiently suppresses growing of rice false smut.
Comparison diagram 1 and Fig. 2, visual compounds wood sugar itself is harmless, and inhibitory action is from the high temperature treatment product of wood sugar, and namely wood sugar decomposes the material generating efficient suppression rice false smut and grow after high temperature treatment.
The rice false smut mortifier deriving from wood sugar disclosed by the invention; its inhibiting object finds to suppress rice false smut at present; according to general Biological Principles; probably this mortifier can also suppress some other growth of pathogenic bacteria to be grown; therefore, the inhibitory action of this mortifier to other pathogen also belongs to protection scope of the present invention.
Claims (1)
1. come from a rice false smut mortifier for wood sugar, it is characterized in that, this mortifier derives from compound wood sugar, is generated after high temperature treatment by wood sugar; This mortifier has effect that strong inhibition rice false smut grows; This mortifier can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510928942.0A CN105309478A (en) | 2015-12-14 | 2015-12-14 | Ustilaginoidea virens inhibitor from xylose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510928942.0A CN105309478A (en) | 2015-12-14 | 2015-12-14 | Ustilaginoidea virens inhibitor from xylose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105309478A true CN105309478A (en) | 2016-02-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510928942.0A Pending CN105309478A (en) | 2015-12-14 | 2015-12-14 | Ustilaginoidea virens inhibitor from xylose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105309478A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779374A (en) * | 2016-05-24 | 2016-07-20 | 广西大学 | Xylose medium suitable for ustilaginoidea virens spore production and application method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594596A (en) * | 2003-09-08 | 2005-03-16 | 余伟 | Production method for xylose |
| CN1867251A (en) * | 2003-09-09 | 2006-11-22 | 3M创新有限公司 | Antimicrobial compositions and methods |
| CN101029060A (en) * | 2007-04-04 | 2007-09-05 | 牛继星 | Hydrolysis for producing high-purity crystallized xylose from corn skin |
-
2015
- 2015-12-14 CN CN201510928942.0A patent/CN105309478A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594596A (en) * | 2003-09-08 | 2005-03-16 | 余伟 | Production method for xylose |
| CN1867251A (en) * | 2003-09-09 | 2006-11-22 | 3M创新有限公司 | Antimicrobial compositions and methods |
| CN101029060A (en) * | 2007-04-04 | 2007-09-05 | 牛继星 | Hydrolysis for producing high-purity crystallized xylose from corn skin |
Non-Patent Citations (3)
| Title |
|---|
| 付建龙 等: "《有机化学》", 30 September 2009 * |
| 张君成 等: "糖对稻曲病菌薄壁分生孢子萌发的影响", 《植物病理学报》 * |
| 荆琪 等: "高温液态水中木糖无催化分解反应动力学", 《化学反应工程与工艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779374A (en) * | 2016-05-24 | 2016-07-20 | 广西大学 | Xylose medium suitable for ustilaginoidea virens spore production and application method thereof |
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Application publication date: 20160210 |