CN102599235A - Volatile preservative for potato wedges - Google Patents

Volatile preservative for potato wedges Download PDF

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Publication number
CN102599235A
CN102599235A CN2012100667567A CN201210066756A CN102599235A CN 102599235 A CN102599235 A CN 102599235A CN 2012100667567 A CN2012100667567 A CN 2012100667567A CN 201210066756 A CN201210066756 A CN 201210066756A CN 102599235 A CN102599235 A CN 102599235A
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China
Prior art keywords
potato
preservative
anticorrisive agent
volatility
volatile
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CN2012100667567A
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Chinese (zh)
Inventor
李梅
田世龙
程建新
李守强
葛霞
张永茂
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Agricultural Products Storage & Processing Institute Gansu Academy Of Agricultural Sciences
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Agricultural Products Storage & Processing Institute Gansu Academy Of Agricultural Sciences
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Priority to CN2012100667567A priority Critical patent/CN102599235A/en
Publication of CN102599235A publication Critical patent/CN102599235A/en
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Abstract

The invention belongs to the field of commercial crops storage, and provides volatile preservative for potato wedges, which comprises, in mass percentage, 20-80% of volatile strong oxidant, 0-0.1% of sodium dodecyl sulfate and the balance of water. The strong oxidant in the preservative can volatilize to a certain concentration in a closed space so as to achieve corrosion prevention. Compared with soaking preservatives, the volatile preservative is washing-free, labor-reducing and more convenient to use. The volatile preservative has an evident inhibitory effect to potato dry rot and soft rot. The preservative respectively reaches 0.56ug/ml and 0.24ug/ml and has 100% of relative inhibitory rate for dry rot and soft rot. Compared with pesticide type preservatives, the volatile preservative is higher in safety.

Description

A kind of volatility potato piece anticorrisive agent
Technical field
The invention belongs to industrial crops storage field, be specifically related to a kind of volatility potato piece anticorrisive agent.
Background technology
In recent years, along with the continuous increase of potato reserves, cause rotten problem of storage of potato phase outstanding day by day, storage disease increases the harm of potato year by year.According to statistics; Because the annual average out to 6~10% of the loss that stem tuber dry rot and soft rot cause; Sometimes even up to 60%; But corresponding antisepsis and sterilization measure also differs greatly, and tracing it to its cause mainly contains the following aspects: 1) holding conditions (mainly referring to facility) is perfect inadequately, lacks the basis of control; 2) anticorrosion and bactericidal agent that uses in the production belongs to pesticide more, and from security, the scope of application is restricted; 3) anticorrisive agent of being researched and developed out belongs to soaked with liquid potato piece class more, because condition restriction lacks operability.Therefore, explore development a kind of safe and efficient, use easy, time saving and energy saving potato piece anticorrisive agent and become the task of top priority.
Summary of the invention
The purpose of this invention is to provide a kind of safe and efficient, use easy, time saving and energy saving volatility potato piece anticorrisive agent.
The present invention realizes that the technical scheme that above-mentioned purpose adopts is following:
A kind of volatility potato piece anticorrisive agent, by mass percentage, the consisting of of this anticorrisive agent: high volatility oxidant 20~80%, dodecyl sodium sulfate 0~0.1% and surplus are water.
Further, said high volatility oxidant is Peracetic acid and/or chlorine dioxide.
The method for preparing above-mentioned volatility potato piece anticorrisive agent adds the dodecyl sodium sulfate of proportional quantity in the entry and to dissolve, and adds the high volatility oxidant of proportional quantity then, mixes and promptly gets described volatility potato piece anticorrisive agent.
The method for using of above-mentioned volatility potato piece anticorrisive agent: the potato piece of gathering is placed confined space; Like the big account of constant temperature storage, storaing kiln or plastics; Put into this anticorrisive agent according to space size and potato piece memory space; Make that it volatilizees naturally, spray volatilization or make its volatilization by miscellaneous equipment behind the thin up, the rear venting ventilation in stifling 24 hours of general sealing and circulating gets final product.
Beneficial effect of the present invention:
(1) volatility potato piece anticorrisive agent of the present invention has the volatility characteristics, and the corrosion-resistant purpose can be realized in strong oxidizer volatilization back wherein after confined space reaches finite concentration; Compare with the anticorrisive agent that soaks class; Need not washing, reduced the amount of labour, use also more convenient.
(2) this volatile corrosion inhibitor all has significant inhibitory effect to dry rot of potato and soft rot; Concentration of preservatives reaches 0.56 μ g/ml and 0.24 μ g/ml respectively the relative inhibition of dry rot and soft rot is reached 100%; Compare with the pesticide anticorrisive agent, security is higher.
The specific embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1
With mass percent is 50% Peracetic acid, 0.1% dodecyl sodium sulfate, and surplus is a water, earlier that dodecyl sodium sulfate is soluble in water, and the back adds Peracetic acid, and mixing gets final product.
Embodiment 2
With mass percent is 50% Peracetic acid, 20% chlorine dioxide, 0.1% dodecyl sodium sulfate, and surplus is a water, earlier that dodecyl sodium sulfate is soluble in water, and the back adds Peracetic acid, and mixing gets final product.
Embodiment 3
With mass percent is 80% chlorine dioxide, 0.1% dodecyl sodium sulfate, and surplus is a water, earlier that dodecyl sodium sulfate is soluble in water, and the back adds chlorine dioxide, and mixing gets final product.
Embodiment 4 anticorrisive agents are to the dry rot of potato isolated test
1) preparation of confession examination bacterial classification
Supply examination dry rot of potato pathogen fusarium sulphureum in preceding 7 days of test, it is subsequent use that the switching activation places 25 ℃ of constant incubators to cultivate.
2) preparation of spore suspension
Get one in 7 days pathogen PDA culture dish of the growth of purifying; Add the about l0ml of sterilized water that contains 0.05% Tween80, the thalline on the culture dish is scraped gently, change over to then in the 50m1 triangular flask with the disinfection inoculation ring; Behind the vibration mixing; Filter with double gauze, filtrating is calculated the concentration of spore suspension with the blood counting chamber counting, be diluted to required concentration 1 * 10 at last 7The bacteria suspension of individual spore/mL is promptly processed required bacteria suspension.
3) the carry disease germs preparation and the assay method of culture medium
Adopt inhibition zone method.Autoclaved agar medium is taken advantage of liquid state pours in the diameter 9cm culture dish of dry sterilization, every ware 30ml, treat the culture medium condensation after, using micropipettor to add concentration is 1 * 10 7Spread plate behind the bacteria suspension 100 μ L of individual spore/mL; Use aseptic metal card punch (d=5mm) to break into the aperture of diameter 5mm then, remove agar in the hole, in the hole, add 1~2 1% agar solution closed hole bottom surface then with aseptic toothpick; Deng in each hole, adding the variable concentrations anticorrisive agent after the agar condensation; Seal with sealing film immediately, each handles 3 repetitions, and with sterilized water as contrast.More than operation is all carried out under aseptic condition.Place 25 ℃ of constant incubators to cultivate 5~7d in culture dish then, when treating that contrast is covered with, measure antibacterial circle diameter.
4) measurement of antibacterial circle diameter and inhibiting rate computational methods
With 2 diameters of right-angled intersection method measurement inhibition zone, average, and calculate the area of inhibition zone.
The computing formula of relative inhibition is following:
Relative inhibition (%)=processing inhibition zone area/contrast growth area * 100
The anticorrisive agent that the used anticorrisive agent of above-mentioned code name 2-1 to 2-6 makes for embodiment 3;
The anticorrisive agent that the used anticorrisive agent of code name 3-1 to 3-6 makes for embodiment 1.
The result shows: potato piece slow release anticorrisive agent has significant inhibitory effect to dry rot of potato, and there is notable difference in the variable concentrations anticorrisive agent to the inhibition of pathogen.In 6 concentration processing that anticorrisive agent supplies respectively to try; Increase along with concentration; Pathogen growth obviously is suppressed, and the germ growth is suppressed fully when liquor strength (high volatility oxidant in soup) reaches 0.56 μ g/ml and 0.69 μ g/ml respectively, and relative inhibition reaches 100%.
Embodiment 5 anticorrisive agents are to the bacterial soft rot of potato isolated test
1) preparation of confession examination bacterial classification
Supply the black shin subspecies of examination bacterial soft rot of potato pathogen carrot soft rot Erwinia potato in preceding 3 days of test, it is subsequent use that the switching activation places 28 ℃ of constant incubators to cultivate.
2) preparation of bacterium bacteria suspension
With the bacterium bacterium liquid of preserving (70 ℃) in the 15% aseptic glycerine; Take the plate streaking partition method, purifying obtains the single bacterium colony of bacterium on beef-protein medium, dips in oese and gets single bacterium colony; Shaking table is cultivated (150rpm in fluid nutrient medium; 37 ℃) 24h, calculate the concentration of bacteria suspension with the blood counting chamber counting, be diluted to required concentration 1 * 10 at last 7The bacteria suspension of individual/mL is promptly processed required bacteria suspension.
3) the carry disease germs preparation and the assay method of culture medium
Adopt inhibition zone method.Autoclaved agar medium is taken advantage of liquid state pours in the diameter 9cm culture dish of dry sterilization, every ware 30ml, treat the culture medium condensation after, using micropipettor to add concentration is 1 * 10 7Spread plate behind the bacteria suspension 100 μ L of individual/mL; Use aseptic metal card punch (d=5mm) to break into the aperture of diameter 5mm then, remove agar in the hole, in the hole, add 1~2 1% agar solution closed hole bottom surface then with aseptic toothpick; Deng in each hole, adding the variable concentrations anticorrisive agent after the agar condensation; Seal with sealing film immediately, each handles 3 repetitions, and with sterilized water as contrast.More than operation is all carried out under aseptic condition.Place 28 ℃ of constant incubators to cultivate 2~3d in culture dish then, when treating that contrast is covered with, measure antibacterial circle diameter.
4) measurement of antibacterial circle diameter and inhibiting rate computational methods
With 2 diameters of right-angled intersection method measurement inhibition zone, average, and calculate the area of inhibition zone.
The computing formula of relative inhibition is following:
Relative inhibition (%)=processing inhibition zone area/contrast growth area * 100
Figure 700237DEST_PATH_IMAGE002
The anticorrisive agent that the used anticorrisive agent of above-mentioned code name 2-1 to 2-6 makes for embodiment 3;
The anticorrisive agent that the used anticorrisive agent of code name 3-1 to 3-6 makes for embodiment 1.
The result shows: potato piece slow release anticorrisive agent has significant inhibitory effect to bacterial soft rot of potato, and there is notable difference in the variable concentrations anticorrisive agent to the inhibition of pathogen.In 6 concentration processing that anticorrisive agent supplies respectively to try; Increase along with concentration; Pathogen growth obviously is suppressed, and the germ growth is suppressed fully when liquor strength (high volatility oxidant in soup) reaches 0.52 μ g/ml and 0.24 μ g/ml respectively, and relative inhibition reaches 100%.

Claims (3)

1. a volatility potato piece anticorrisive agent is characterized in that, by mass percentage, the consisting of of said volatility potato piece anticorrisive agent: high volatility oxidant 20~80%, dodecyl sodium sulfate 0~0.1% and surplus are water.
2. according to the said volatility potato of claim 1 piece anticorrisive agent, it is characterized in that: said high volatility oxidant is Peracetic acid and/or chlorine dioxide.
3. the method for preparing the said volatility potato of claim 1 piece anticorrisive agent; It is characterized in that: the dodecyl sodium sulfate of proportional quantity is added in the entry dissolve; The high volatility oxidant that adds proportional quantity then mixes and promptly gets described volatility potato piece anticorrisive agent.
CN2012100667567A 2012-03-14 2012-03-14 Volatile preservative for potato wedges Pending CN102599235A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103931753A (en) * 2014-05-05 2014-07-23 甘肃省农业科学院农产品贮藏加工研究所 Preservative special for tuber storage as well as preparation method and application method of preservative
CN104885941A (en) * 2015-05-26 2015-09-09 福建农林大学 Chemical disinfection tissue culture method for miscanthus giganteus
CN105410155A (en) * 2015-11-27 2016-03-23 湖北工业大学 Color-protecting and fresh-keeping method capable of restraining potatoes from sprouting and reducing solanine
CN106596492A (en) * 2016-12-28 2017-04-26 商丘市农林科学院 Method for identifying infection resistance of peanuts to aspergillus flavus and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1442062A (en) * 2003-03-08 2003-09-17 湘潭大学 Agricultural and sideline product mildew proof and antistaling agent
CN1640275A (en) * 2004-01-06 2005-07-20 新疆大学 Chlorine dioxide antistaling agent
CN101822286A (en) * 2010-06-02 2010-09-08 张俊 Preservation method of fresh-cut potatoes
CN101990932A (en) * 2009-08-14 2011-03-30 甘肃省农业科学院农产品贮藏加工研究所 Fresh-keeping processing technology of fast-food fresh-cutting potato

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1442062A (en) * 2003-03-08 2003-09-17 湘潭大学 Agricultural and sideline product mildew proof and antistaling agent
CN1640275A (en) * 2004-01-06 2005-07-20 新疆大学 Chlorine dioxide antistaling agent
CN101990932A (en) * 2009-08-14 2011-03-30 甘肃省农业科学院农产品贮藏加工研究所 Fresh-keeping processing technology of fast-food fresh-cutting potato
CN101822286A (en) * 2010-06-02 2010-09-08 张俊 Preservation method of fresh-cut potatoes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NORA L. OLSEN, ET AL.: "Efficacy of Chlorine Dioxide for Disease Control on Stored Potatoes", 《AMERICAN JOURNAL OF POTATO RESEARCH》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103931753A (en) * 2014-05-05 2014-07-23 甘肃省农业科学院农产品贮藏加工研究所 Preservative special for tuber storage as well as preparation method and application method of preservative
CN104885941A (en) * 2015-05-26 2015-09-09 福建农林大学 Chemical disinfection tissue culture method for miscanthus giganteus
CN105410155A (en) * 2015-11-27 2016-03-23 湖北工业大学 Color-protecting and fresh-keeping method capable of restraining potatoes from sprouting and reducing solanine
CN106596492A (en) * 2016-12-28 2017-04-26 商丘市农林科学院 Method for identifying infection resistance of peanuts to aspergillus flavus and application thereof

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Application publication date: 20120725