CN103141470A - Use of chitosan quaternary ammonium salt as specimen anti-mildew agent - Google Patents
Use of chitosan quaternary ammonium salt as specimen anti-mildew agent Download PDFInfo
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- CN103141470A CN103141470A CN2013100854731A CN201310085473A CN103141470A CN 103141470 A CN103141470 A CN 103141470A CN 2013100854731 A CN2013100854731 A CN 2013100854731A CN 201310085473 A CN201310085473 A CN 201310085473A CN 103141470 A CN103141470 A CN 103141470A
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Abstract
The invention relates to use of chitosan quaternary ammonium salt as a specimen anti-mildew agent, and belongs to the technical field of specimen mildew preventives. A mildew preventive storage liquid is a solution with total molar concentration of chitosan quaternary ammonium salt of 0.010-100.0 mol/L. The application method comprises the following step of: coating/soaking/or spraying a specimen by use of the solution to prevent mildew for the specimen. The chitosan quaternary ammonium salt is a completely safe/environmental-friendly specimen mildew preventing and treating method. The storage liquid does not contain ethanol and formaldehyde and is free from toxic and side effects to a human body and environment, and can be used within minus 40-40 DEG C, and the specimen is maintained originally. The specimen anti-mildew agent can better inhibit propagation of bacteria and mildews in a neutral condition and has good storing, fixing, sterilizing and mildew preventing effects to specimens. The raw materials of the storage liquid are low-toxic environmental-friendly chemicals, so that the storage liquid is less in damage to a human body and environment and is an ideal substitute product for formalin.
Description
Technical field
The present invention relates to a kind of sample mould inhibitor and application method thereof, be specifically related to chitosan quaternary ammonium salt as the purposes of sample anti-mildew agent, belong to sample mould inhibitor technical field.
Background technology
The samples such as miscellaneous animal, plant, historical relic are subject to the most serious infringement because of the harm of mould, and sample is shortened dramatically service life, also cause the tremendous economic loss simultaneously.Zoological specimens can grow white mould after oozing out grease very soon.White mould is a kind of fungi, and it is attached on the surface of sample, makes sample skin become broken, rot.Present sample dissipation mould method is only used the chemicals method, mainly contains formalin-potassium permanganate fumigation, O-SyL thymol, N-phenylsalicylamide etc.In a single day sample goes mouldy, then with chemicals method sterilizations such as formaldehyde, poor effect illustrates that mould has produced drug resistance to chemicals.Not only can not accomplish to control fully the effect of sample mould. simultaneously the healthy of staff and visitor caused serious injury.Therefore seek safety non-toxic, non-secondary pollution, the sample mould inhibitor of safety and environmental protection has been extremely urgent.
At present mouldy with the sample of animal infuse is the thorny problem that the mechanisms such as medical teaching, clinical pathology check, legal medical expert, zooscopy, museum run into always.The animal humid preparation is usually after anticorrosion fixedly processing, be soaked in the preservation of wetting in 5% formalin (main component is formaldehyde) solution, though antiseptic effect is good, but the stimulation to the human body mucous membrane is large, and pollution surrounding environment, and in use the situation that the sample exposed parts goes mouldy can appear, the thin and transparent mould film of one deck also can appear in liquid level.The sample that oozes out grease can grow white mould very soon.White mould belongs to fungi, and it is attached on the surface of sample, makes sample skin become broken, rot.In a single day sample goes mouldy, then uses formaldehyde sterilization, especially mould, and poor effect illustrates that the mould PARA FORMALDEHYDE PRILLS(91,95) has produced drug resistance.Common mould mainly contains aspergillus candidus, black-koji mould and Aspergillus flavus etc.
Sample goes mouldy and has brought many impacts to exhibition, teaching, science popularization, scientific research, makes it lose use value, and the staff contacts for a long time, in a large number mould and also can endanger healthy.Although other preservatives also have some reports, as Sodium Benzoate, potassium sorbate, calcium propionate, sodium Diacetate and phenol etc., all can not reach the antiseptic effect of formalin at present.
The animal skinned-specimen problem of going rotten of damaging by worms has had a strong impact on the preservation of sample, but the research of Vermins-proof mildew-proof new material and new method is relative less, and the mould inhibitor of novel safety and environmental protection is also rarely found, so research and develop novel mould inhibitor, very important meaning is arranged.
Chitosan quaternary ammonium salt is a kind of environment-friendly type macromolecule polymer disinfection sanitizer, has the sterilization wide spectrum; Valid density is low; Speed of action is fast; Stable in properties; Premium properties soluble in water; Can use at normal temperatures; For a long time antibacterial, have no side effect; Non-corrosiveness; Colourless, odorless; Nontoxic; Do not fire, not quick-fried, use safety; Moderate; Convenient transportation can be described as best bactericide.Chitosan quaternary ammonium salt is the disinfecting, antimicrobial component, but the fission function of anti-bacteria, virus, and the respiration channel of obstruction microorganism reaches sterilization effect.After adding chitosan quaternary ammonium salt, the cytoplasm of bacterium is assembled obviously, and very significantly plasmolysis is arranged, and cell wall is fuzzy, excalation, and the defencive function of the cell wall of Escherichia coli and gold-coloured staphylococci obviously descends, and a large amount of desmoenzymes leak.This is because chitosan quaternary ammonium salt is typical cationic high polymer, is strong cation and antibacterial in solution.
Chitosan quaternary ammonium salt is a kind of environment-friendly multipurpose new polymers, in industrial, agriculture, medical and daily life, purposes is extremely widely arranged.But chitosan quaternary ammonium salt there is not yet report as the sample mould inhibitor.So research uses chitosan quaternary ammonium salt as the sample mould inhibitor, safety non-toxic, non-secondary pollution, environmental protection, to the protection sample, economic loss and to protect mankind health that the samples such as minimizing animal, plant, historical relic cause because go mouldy have important practical significance.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art; the purposes of chitosan quaternary ammonium salt as the sample anti-mildew agent is provided; this mould inhibitor is a kind of environment-friendly type sample mould inhibitor; this mould inhibitor safety non-toxic; non-secondary pollution; the economic loss that sample causes because of going mouldy can be reduced, the healthy of to protect mankind can be reached simultaneously.
To achieve these goals, the technical solution adopted in the present invention is:
1. chitosan quaternary ammonium salt is as the purposes of sample anti-mildew agent; Described mould inhibitor is comprised of chitosan quaternary ammonium salt; The concentration of described chitosan quaternary ammonium salt is 0.010～100.0 mol/L; Described purposes be with the chitosan quaternary ammonium salt that concentration is 0.010～100.0 mol/L smear soak or the spray sample, play the sample mildew-proof function.
The bacteriostatic test of mould inhibitor:
1. inoculation activation
First after the mould collection with the sample body surface, cultivate under 30 ℃ with PDA and cultivate, then first with mould inoculation activation, the test tube slant mycotic spore is washed with aqua sterilisa, draw bacteria suspension 200mL with liquid-transfering gun and add the PDA plating medium, spreading rod is coated with bacteria suspension evenly (spreading rod is burnt sterilization after washing with 95% ethanol or acetaldehyde in advance again on alcolhol burner), after placing 5min, with cultivating 24-48h in 30 ℃ of flat board storings, biochemical cultivation case, grow out without can be used as the seed seedling after miscellaneous bacteria;
2. inhibition experimental section
(1) chitosan quaternary ammonium salt presses down the experiment of sample mould
Chitosan quaternary ammonium salt is diluted to 0.05%(3 with sterile water), 0.1%(3), 0.2%(3), 0.3%(3), blank group (1), each concentration follpet plug test tube of packing into;
Some with scissors clip diameter 0.7cm filter paper, put 121 ℃, 30min sterilization in test tube.To plant daughter bacteria according to three steps again by flat board approximately 10, every kind concentration filter paper get 12 with the sterilization tweezers, inoculate respectively 3 flat boards, cultivate 48-72h for 30 ℃, observe inhibition zone big or small, turn over and use ruler measurement;
(2) formaldehyde (formalin) suppresses experiment
With doing check experiment with concentration 0.05%, 0.1%, 0.2%, 0.3% formalin, method is the same.
The invention has the beneficial effects as follows:
This be a kind of overall safety the sample control mould method of environmental protection;
2. this kind mould inhibitor does not contain ethanol and formaldehyde, and human body and environment are had no side effect, and sample is kept intact, and can use in-40 ℃～40 ℃ scopes;
3. this sample anti-mildew agent better growing of anti-bacteria and mould just under neutrallty condition has good preservation, fixing, sterilization, mildew-proof function to sample; And this preserves the liquid raw material and is nontoxic environmental-friendly natural active matter, and is harmless to human body and Environmental security, is the desirable substitute products of formalin.
Description of drawings
Fig. 1 is that chitosan quaternary ammonium salt suppresses sample mould fungistatic effect figure, antibacterial circle diameter: 1.5～1.8;
Fig. 2 is for being mould negative control figure;
Fig. 3 is that the 100PPM chitosan quaternary ammonium salt suppresses sample green mold fungistatic effect figure, antibacterial circle diameter: 1.9～2.1;
Fig. 4 is for being the mould negative control;
Fig. 5 is that 100PPM formaldehyde suppresses sample Penicillium notatum design sketch, antibacterial circle diameter: 0.5～0.8cm.
Embodiment
Below by embodiment, the present invention is described in further details, these embodiment only are used for illustrating the present invention, do not limit the scope of the invention.
Embodiment 1
Adopt the inventive method to follow these steps to carry out chitosan quaternary ammonium salt and suppress the experiment of sample Penicillium notatum, antibacterial circle diameter: 1.5～1.8;
1. inoculation activation
The test tube slant mycotic spore is washed with aqua sterilisa, draw bacteria suspension 200mL with liquid-transfering gun and add the PDA plating medium, spreading rod is coated with bacteria suspension evenly (spreading rod is burnt sterilization after washing with 95% ethanol or acetaldehyde in advance again on alcolhol burner).After placing 5min.To cultivate 24-48h in 30 ℃ of flat board storings, biochemical cultivation case.Grow out without can be used as the seed seedling after miscellaneous bacteria;
2. inhibition experimental section
(1) chitosan quaternary ammonium salt presses down the experiment of sample mould
Chitosan quaternary ammonium salt is diluted to 0.05%(3 with sterile water), 0.1%(3), 0.2%(3), 0.3%(3), blank group (1), each concentration follpet plug test tube of packing into;
Some with scissors clip diameter 0.7cm filter paper, put 121 ℃, 30min sterilization in test tube.To plant daughter bacteria according to three steps again by flat board approximately 10, every kind concentration filter paper get 12 with the sterilization tweezers, inoculate respectively 3 flat boards, cultivate 48-72h for 30 ℃, observe inhibition zone big or small, turn over and use ruler measurement;
(2) formaldehyde (formalin) suppresses experiment
With doing check experiment with concentration 0.05%, 0.1%, 0.2%, 0.3% formalin, method is the same.
Embodiment 2
Adopt the inventive method to follow these steps to carry out chitosan quaternary ammonium salt and suppress fish sample green mold:
1, inoculation activation
The test tube slant mycotic spore is washed with aqua sterilisa, draw bacteria suspension 200mL with liquid-transfering gun and add the PDA plating medium, spreading rod is coated with bacteria suspension evenly (spreading rod is burnt sterilization after washing with 95% ethanol or acetaldehyde in advance again on alcolhol burner), after placing 5min, will cultivate 24-48h in 30 ℃ of flat board storings, biochemical cultivation case.Grow out without can be used as the seed seedling after miscellaneous bacteria;
2. inhibition experimental section
(1) chitosan quaternary ammonium salt presses down the experiment of sample green mold mould
Chitosan quaternary ammonium salt is diluted to 0.05%(3 with sterile water), 0.1%(3), 0.2%(3), 0.3%(3), blank group (1), each concentration follpet plug test tube of packing into;
Some with scissors clip diameter 0.7cm filter paper.Put 121 ℃, 30min sterilization in test tube.To plant daughter bacteria according to three steps again by flat board approximately 10, every kind concentration filter paper get 12 with the sterilization tweezers, inoculate respectively 3 flat boards, cultivate 48-72h for 30 ℃, observe inhibition zone big or small, turn over and use ruler measurement;
(2) formaldehyde (formalin) suppresses experiment
With doing check experiment with concentration 0.05%, 0.1%, 0.2%, 0.3% formalin, method is the same.
Embodiment 3
Adopt the inventive method to follow these steps to carry out chitosan quaternary ammonium salt and suppress the experiment of fish sample aspergillus niger mould:
This process method step is:
First after the mould collection with the sample body surface, cultivate under 30 ℃ with PDA and cultivate, then first with mould inoculation activation, the test tube slant mycotic spore is washed with aqua sterilisa, draw bacteria suspension 200mL with liquid-transfering gun and add the PDA plating medium, spreading rod is coated with bacteria suspension evenly (spreading rod is burnt sterilization after washing with 95% ethanol or acetaldehyde in advance again on alcolhol burner), after placing 5min, with cultivating 24-48h in 30 ℃ of flat board storings, biochemical cultivation case, grow out without can be used as the seed seedling after miscellaneous bacteria;
Chitosan quaternary ammonium salt is diluted to 0.05%(3 with sterile water), 0.1%(3), 0.2%(3), 0.3%(3), blank group (1), each concentration follpet plug test tube of packing into;
Some with scissors clip diameter 0.7cm filter paper, put 121 ℃, 30min sterilization in test tube.To plant daughter bacteria according to three steps again by flat board approximately 10, every kind concentration filter paper get 12 with the sterilization tweezers, inoculate respectively 3 flat boards, cultivate 48-72h for 30 ℃, observe inhibition zone big or small, turn over and use ruler measurement; The chitosan quaternary ammonium salinity is: 0.010～100.0 mol/L;
Design simultaneously formaldehyde as a control group, experimental technique is the same.
Embodiment 4
Adopt the inventive method to follow these steps to carry out chitosan quaternary ammonium salt and suppress fish sample aspergillus albicans inhibitory action
First after the mould collection with the sample body surface, cultivate under 30 ℃ with PDA and cultivate, then first with mould inoculation activation, the test tube slant mycotic spore is washed with aqua sterilisa, draw bacteria suspension 200mL with liquid-transfering gun and add the PDA plating medium, spreading rod is coated with bacteria suspension evenly (spreading rod is burnt sterilization after washing with 95% ethanol or acetaldehyde in advance again on alcolhol burner), after placing 5min, with cultivating 24-48h in 30 ℃ of flat board storings, biochemical cultivation case, grow out without can be used as the seed seedling after miscellaneous bacteria;
Chitosan quaternary ammonium salt is diluted to 0.05%(3 with sterile water), 0.1%(3), 0.2%(3), 0.3%(3), blank group (1), each concentration follpet plug test tube of packing into;
Some with scissors clip diameter 0.7cm filter paper, put 121 ℃, 30min sterilization in test tube.To plant daughter bacteria according to three steps again by flat board approximately 10, every kind concentration filter paper get 12 with the sterilization tweezers, inoculate respectively 3 flat boards, cultivate 48-72h for 30 ℃, observe inhibition zone big or small, turn over and use ruler measurement; The chitosan quaternary ammonium salinity is: 0.010～100.0 mol/L;
Design simultaneously formaldehyde as a control group, experimental technique is the same.
The experimental result contrast
As shown in the figure, adopt chitosan quaternary ammonium salt to press down the zoological specimens mould, fungistatic effect is obvious, can effectively suppress the growth of the mould in zoological specimens, 100PPM formaldehyde suppress sample Penicillium notatum effect than the 100PPM chitosan quaternary ammonium salt suppress sample mould mould weak effect some, the intense stimulus smell is arranged simultaneously.
Embodiment 5
With the concentration chitosan quaternary ammonium salt that is 0.010 mol/L smear soak or the spray sample, play the sample mildew-proof function.
Embodiment 6
With the concentration chitosan quaternary ammonium salt that is 100.0 mol/L smear soak or the spray sample, play the sample mildew-proof function.
Embodiment 7
With the concentration chitosan quaternary ammonium salt that is 50.0 mol/L smear soak or the spray sample, play the sample mildew-proof function.
Claims (4)
1. chitosan quaternary ammonium salt as the purposes of sample anti-mildew agent, is characterized in that: chitosan quaternary ammonium salt is as the purposes of zoological specimens mould inhibitor.
2. chitosan quaternary ammonium salt according to claim 1 as the purposes of sample anti-mildew agent, is characterized in that: described mould inhibitor is comprised of chitosan quaternary ammonium salt.
3. chitosan quaternary ammonium salt according to claim 2 as the purposes of sample anti-mildew agent, is characterized in that: the concentration of described chitosan quaternary ammonium salt is 0.010～100.0 mol/L.
4. chitosan quaternary ammonium salt according to claim 1 is as the purposes of sample anti-mildew agent, it is characterized in that: described purposes be with the chitosan quaternary ammonium salt that concentration is 0.010～100.0 mol/L smear soak or the spray sample, play the sample mildew-proof function.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994370A (en) * | 2016-05-21 | 2016-10-12 | 杨勇 | Novel mould inhibitor |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994370A (en) * | 2016-05-21 | 2016-10-12 | 杨勇 | Novel mould inhibitor |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
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Application publication date: 20130612 |